Swetha Ananth, Ina Ambiel, Sandra Schifferdecker, Thorsten G Müller, Paul R Wratil, Ernesto Mejias-Perez, Hans-Georg Kräusslich, Barbara Müller, Oliver T Keppler, Oliver T Fackler
Resting CD4 T cells resist productive HIV-1 infection. The HIV-2/simian immunodeficiency virus protein viral accessory protein X (Vpx) renders these cells permissive to infection, presumably by alleviating blocks at cytoplasmic reverse transcription and subsequent nuclear import of reverse-transcription/pre-integration complexes (RTC/PICs). Here, spatial analyses using quantitative virus imaging techniques reveal that HIV-1 capsids containing RTC/PICs are readily imported into the nucleus, recruit the host dependency factor CPSF6, and translocate to nuclear speckles in resting CD4 T cells...
March 12, 2024: Cell Reports