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https://www.readbyqxmd.com/read/28433186/characterization-and-thermal-inactivation-kinetics-of-highly-thermostable-ramie-leaf-%C3%AE-amylase
#1
Liqin He, Sung-Hoon Park, Nguyen Dang Hai Dang, Hoa Xo Duong, Thi Phung Cac Duong, Phuong Lan Tran, Jong-Tae Park, Li Ni, Kwan-Hwa Park
We characterized ramie leaf β-amylase, and determined its thermostability and kinetic parameters. The enzyme was purified 53-fold using ammonium sulfate fractionation (40-60% saturation), anion exchange chromatography on DEAE-cellulose and gel permeation chromatography on Superdex-200. The purified enzyme was identified as β-amylase with molecular mass of 42kD. The enzyme displayed Km and kcat values for soluble potato starch of 1.1mg/mL and 7.8s(-1), respectively. The enzyme had a temperature optimum of 65°C, and its activity at 70°C was 92% of that at the optimal temperature after a 15-min incubation...
June 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28432667/fully-automated-analysis-of-perfusion-data-the-rise-of-the-machines
#2
EDITORIAL
Rupa M Sanghani, Rami Doukky
No abstract text is available yet for this article.
April 21, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/28432612/evaluation-of-the-analgesia-nociception-index-ani-in-healthy-awake-volunteers
#3
Rami Issa, Marco Julien, Elizabeth Décary, Olivier Verdonck, Louis-Philippe Fortier, Pierre Drolet, Philippe Richebé
BACKGROUND: Analgesia might be evaluated with simple changes in vital signs, a non-specific and non-sensitive method. Heart rate variability (HRV) correlates with autonomous nervous system activity and can be used to evaluate painful stimuli. Heart rate variability is then transformed into a numeric scale called the analgesia nociception index (ANI), where higher values represent predominant parasympathetic tone, thus low nociception. Under general anesthesia, the ANI decreases following painful stimuli and increases after administration of analgesia, but significant interindividual variability is present...
April 21, 2017: Canadian Journal of Anaesthesia, Journal Canadien D'anesthésie
https://www.readbyqxmd.com/read/28432603/immunoprophylaxis-of-multi-antigen-peptide-map-vaccine-for-human-lymphatic-filariasis
#4
Christiana Immanuel, Aparnaa Ramanathan, Malathi Balasubramaniyan, Vishal Kishor Khatri, Nitin Purushottam Amdare, Donthamsetty Nageswara Rao, Maryada Venkata Rami Reddy, Kaliraj Perumal
Human lymphatic filariasis, the parasitic disease caused by the filarial nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori, is ranked as the second most complex clinical condition leading to permanent and long-term disability. The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T and B cell epitopes as the constituents of a single immunogen. Here, we report on the design, chemical synthesis, and immunoprophylaxis of three epitopes that have been identified from promising vaccine candidates reported in our previous studies, constructed as MAP on an inert lysine core for human lymphatic filariasis in Jird model...
April 21, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28432192/evidence-that-avian-influenza-vaccination-induces-long-lived-immune-responses-in-zoo-birds
#5
H Fernández-Bellon, J Vergara-Alert, V Almagro, R Rivas, N Majó, N Busquets, A Ramis
No abstract text is available yet for this article.
April 21, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28429574/flip-angle-optimization-for-balanced-ssfp-cardiac-cine-imaging-following-the-application-of-standard-extracellular-contrast-agent-gadobutrol
#6
Daniel L R Kuetting, Darius Dabir, Julian Luetkens, Andreas Feisst, Rami Homsi, Daniel Thomas, Hans H Schild, Alois M Sprinkart
PURPOSE: To investigate the effect of the flip angle (FA) on the blood-myocardium contrast and to define the FA leading to highest image quality in contrast-enhanced balanced steady-state free precession (bSSFP) images. bSSFP images provide excellent contrast between myocardium and blood with high signal-to-noise and contrast-to-noise ratios (SNR, CNR). In clinical practice, bSSFP images are typically acquired following the injection of extracellular contrast agents (ECAs), although ECAs decrease the blood-myocardium contrast...
April 21, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28429200/achalasia-it-is-not-all-black-and-white
#7
REVIEW
Santosh Sanagapalli, Rami Sweis
PURPOSE OF REVIEW: This review aims to shed light on subtleties of achalasia diagnosis, including potential pitfalls that may lead to errors. Optimal methods for assessment of disease severity and the relationship between achalasia and other motility disorders will also be reviewed with an emphasis on recent findings from the literature. RECENT FINDINGS: Adjunctive testing with viscous substances or larger water volumes should be used routinely as it improves the accuracy of achalasia diagnosis...
June 2017: Current Gastroenterology Reports
https://www.readbyqxmd.com/read/28425244/genetic-association-analysis-of-erbb4-polymorphisms-with-the-risk-ofschizophrenia-susceptibility-in-a-jordanian-population-of-arab-descent
#8
Laith Al-Eitan, Sahar Al-Habahbeh, Rami Alkhatib
BACKGROUND/AIM: The ERBB4 gene encodes a transmembrane tyrosine kinase and is considered to be one of the risk genes of schizophrenia. Although there is evidence of the roles of genes and the environment in the etiology of schizophrenia, a comprehensive biological and genetic background of the disease is still lacking. The aim of this study is to assess whether genetic variation in the human ERBB4 gene is associated with vulnerability to schizophrenia in the Jordanian Arab population...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28420213/folate-decorated-nanomicelles-loaded-with-a-potent-curcumin-analogue-for-targeting-retinoblastoma
#9
Hashem Alsaab, Rami M Alzhrani, Prashant Kesharwani, Samaresh Sau, Sai Hs Boddu, Arun K Iyer
The aim of this study was to develop a novel folate receptor-targeted drug delivery system for retinoblastoma cells using a promising anticancer agent, curcumin-difluorinated (CDF), loaded in polymeric micelles. Folic acid was used as a targeting moiety to enhance the targeting and bioavailability of CDF. For this purpose, amphiphilic poly(styrene-co-maleic acid)-conjugated-folic acid (SMA-FA) was synthesized and utilized to improve the aqueous solubility of a highly hydrophobic, but very potent anticancer compound, CDF, and its targeted delivery to folate overexpressing cancers...
April 18, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/28419023/interrelations-of-epicardial-fat-volume-left-ventricular-t1-relaxation-times-and-myocardial-strain-in-hypertensive-patients-a-cardiac-magnetic-resonance-study
#10
Rami Homsi, Daniel Kuetting, Alois Sprinkart, Nina Steinfeld, Michael Meier-Schroers, Julian Luetkens, Jennifer Nadal, Darius Dabir, Stefan Fischer, Juergen Gieseke, Hans Schild, Daniel Thomas
PURPOSE: This cardiac magnetic resonance study was performed to assess myocardial fibrosis by evaluating T1-relaxation time (T1), to measure left ventricular (LV) strain, and to determine epicardial fat volume (EFV) in hypertensive patients with no history of cardiovascular (CV) events and to relate the results to the presence of coronary atherosclerotic artery disease (CAD) in these patients. MATERIALS AND METHODS: A total of 123 subjects were examined at 1.5 T...
May 2017: Journal of Thoracic Imaging
https://www.readbyqxmd.com/read/28414870/three-dimensional-skeletal-mandibular-changes-associated-with-herbst-appliance-treatment
#11
B Q Souki, P L C Vilefort, D D Oliveira, I Andrade, A C Ruellas, M S Yatabe, T Nguyen, L Franchi, J A McNamara, L H S Cevidanes
OBJECTIVES: Three-dimensional evaluation of skeletal mandibular changes following Herbst appliance treatment. SETTING AND SAMPLE POPULATION: Retrospective case-control study, based on a sample size calculation. Twenty-five pubertal patients treated with Herbst appliance (HAG), and 25 matched Class II patients who received other non-orthopaedic dental treatments (CG). MATERIAL AND METHODS: Three-dimensional models were generated from pre-treatment (T0) and post-treatment (T1) cone beam computed tomograms...
May 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28409337/surgical-considerations-with-the-operative-fixation-of-unstable-paediatric-pelvic-ring-injuries
#12
Mohamed Kenawey
INTRODUCTION: The operative treatment of unstable pelvic injuries in paediatrics is not frequently indicated. The detailed modes of pelvic ring failure, surgical techniques, fixation choices, and peri-operative difficulties are not well reported. METHODS: From September 2010 to March 2016, 62 paediatric patients were admitted to an academic level I trauma center with the diagnosis of pelvic ring injury. Of them, 29 (17 males and 12 females) had operative fixation of unstable pelvic injuries...
April 13, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28407696/runx1-a-new-regulator-of-emt-in-breast-cancer
#13
Saleh Khawaled, Rami I Aqeilan
No abstract text is available yet for this article.
March 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28406049/evaluation-of-reduced-dose-ct-for-acute-non-traumatic-abdominal-pain-evaluation-of-diagnostic-accuracy-in-comparison-to-standard-dose-ct
#14
Ahmed E Othman, Malte Niklas Bongers, Dominik Zinsser, Christoph Schabel, Julian L Wichmann, Rami Arshid, Mike Notohamiprodjo, Konstantin Nikolaou, Fabian Bamberg
Background Patients with acute non-traumatic abdominal pain often undergo abdominal computed tomography (CT). However, abdominal CT is associated with high radiation exposure. Purpose To evaluate diagnostic performance of a reduced-dose 100 kVp CT protocol with advanced modeled iterative reconstruction as compared to a linearly blended 120 kVp protocol for assessment of acute, non-traumatic abdominal pain. Material and Methods Two radiologists assessed 100 kVp and linearly blended 120 kVp series of 112 consecutive patients with acute non-traumatic pain (onset < 48 h) regarding image quality, noise, and artifacts on a five-point Likert scale...
January 1, 2017: Acta Radiologica
https://www.readbyqxmd.com/read/28403142/melatonin-protects-rats-from-radiotherapy-induced-small-intestine-toxicity
#15
Beatriz Fernández-Gil, Ahmed E Abdel Moneim, Francisco Ortiz, Ying-Qiang Shen, Viviana Soto-Mercado, Miguel Mendivil-Perez, Ana Guerra-Librero, Darío Acuña-Castroviejo, María M Molina-Navarro, José M García-Verdugo, Ramy K A Sayed, Javier Florido, Juan D Luna, Luis Carlos López, Germaine Escames
Radiotherapy-induced gut toxicity is among the most prevalent dose-limiting toxicities following radiotherapy. Prevention of radiation enteropathy requires protection of the small intestine. However, despite the prevalence and burden of this pathology, there are currently no effective treatments for radiotherapy-induced gut toxicity, and this pathology remains unclear. The present study aimed to investigate the changes induced in the rat small intestine after external irradiation of the tongue, and to explore the potential radio-protective effects of melatonin gel...
2017: PloS One
https://www.readbyqxmd.com/read/28400698/laparoscopic-rectopexy-with-urinary-bladder-xenograft-reinforcement
#16
Aradhana Mehta, Rami Afshar, David L Warner, Amy Gardner, Ellen Ackerman, Jared Brandt, Kent C Sasse
BACKGROUND AND OBJECTIVES: Rectal prolapse is often repaired laparoscopically, frequently with the use of reinforcement material. Both synthetic and biologically derived materials reduce recurrence rate compared to primary suture repair. Synthetic mesh introduces potential complications such as mesh erosion, fibrosis, and infection. Urinary bladder matrix (UBM) represents a biologically derived material for reinforcement of rectal prolapse repair with the potential to improve durability without risks of synthetic materials...
January 2017: JSLS: Journal of the Society of Laparoendoscopic Surgeons
https://www.readbyqxmd.com/read/28399867/the-protracted-waste-crisis-and-physical-health-of-workers-in-beirut-a-comparative-cross-sectional-study
#17
Rami Z Morsi, Rawan Safa, Serge F Baroud, Cherine N Fawaz, Jad I Farha, Fadi El-Jardali, Monique Chaaya
BACKGROUND: Since July 2015, Lebanon has been experiencing a waste management crisis. Dumpsites in inhabited areas and waste burning have emerged due to the waste accumulation, further adding to the gravity of the situation. However, the association between the crisis and health of the population has not been scientifically reported. METHODS: A comparative cross-sectional study was conducted to assess whether exposure to open dumpsites and waste burning is associated with acute health symptoms...
April 11, 2017: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/28396472/characterizing-risk-of-ebola-transmission-based-on-frequency-and-type-of-case-contact-exposures
#18
Laura A Skrip, Mosoka P Fallah, Stephen G Gaffney, Rami Yaari, Dan Yamin, Amit Huppert, Luke Bawo, Tolbert Nyenswah, Alison P Galvani
During the initial months of the 2013-2016 Ebola epidemic, rapid geographical dissemination and intense transmission challenged response efforts across West Africa. Contextual behaviours associated with increased risk of exposure included travel to high-transmission settings, caring for sick and preparing the deceased for traditional funerals. Although such behaviours are widespread in West Africa, high-transmission pockets were observed. Superspreading and clustering are typical phenomena in infectious disease outbreaks, as a relatively small number of transmission chains are often responsible for the majority of events...
May 26, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28394151/school-related-experiences-of-adolescents-in-foster-care-a-comparison-with-their-high-school-peers
#19
Rami Benbenishty, Alana Siegel, Ron Avi Astor
Although adolescents in foster care are known to be more at risk for school-related academic challenges, there is a paucity of research on their school-related experiences, such as victimization and relationships with teachers, compared with their same-age peers not in care. The aim of this article is to compare foster-care adolescents and their schoolmates on data that was drawn from the statewide representative California Healthy Kids survey and includes 165,815 nonfoster youth and 706 foster youth in 9th and 11th grades...
April 10, 2017: American Journal of Orthopsychiatry
https://www.readbyqxmd.com/read/28393023/mathematical-analysis-of-corneal-remodelling-after-intracorneal-ring-surgery-in-keratoconus
#20
Elias F Jarade, Elise Slim, Carole Cherfan, Hala El Rami, Toufic Hassan, Elias Chelala
AIM: To represent mathematically the intersection between the ectatic corneal geometry and the plane of intracorneal ring implants (ICRS) in order to determine the corneal response to ICRS surgery in keratoconus (KC). Thereafter, to present the concept and early results of a newly derived topography-guided nomogram for ICRS surgery for the treatment of keratectasia. METHODS: The corneal rings plane intersection was modelled to a conic section. Ring effect was the result of: the ring size, position (steep vs flat), location (distance from the geometric centre of the cornea), and the discrepancy between the ring's curvature and the tunnel's curvature...
2017: International Journal of Ophthalmology
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