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https://www.readbyqxmd.com/read/28926627/tiger-sharks-can-connect-equatorial-habitats-and-fisheries-across-the-atlantic-ocean-basin
#1
André S Afonso, Ricardo Garla, Fábio H V Hazin
Increasing our knowledge about the spatial ecology of apex predators and their interactions with diverse habitats and fisheries is necessary for understanding the trophic mechanisms that underlie several aspects of marine ecosystem dynamics and for guiding informed management policies. A preliminary assessment of tiger shark (Galeocerdo cuvier) population structure off the oceanic insular system of Fernando de Noronha (FEN) and the large-scale movements performed by this species in the equatorial Atlantic Ocean was conducted using longline and handline fishing gear and satellite telemetry...
2017: PloS One
https://www.readbyqxmd.com/read/28923715/a-multiplex-bead-based-assay-for-immune-gene-expression-analysis-in-shrimp
#2
Sopacha Arayamethakorn, Nitsara Karoonuthaisiri, Wanilada Rungrassamee
Here, we developed a 9-plex bead-based array as a tool to evaluate molecular effects on transcription levels of immune-related genes in the black tiger shrimp (Penaeus monodon). The bead array technology allows simultaneous detection of multiple target genes in a single sample, reducing time, labor and cost. The oligonucleotide probes were designed to target eight immune-related genes that involve in antimicrobial activity, melanization, pathogen pattern recognition proteins, lysozyme and one housekeeping gene as an internal control...
September 15, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28919326/characterization-of-a-pkr-inhibitor-from-the-pathogenic-ranavirus-ambystoma-tigrinum-virus-using-a-heterologous-vaccinia-virus-system
#3
Trung P Huynh, James K Jancovich, Latha Tripuraneni, Michael C Heck, Jeffrey O Langland, Bertram L Jacobs
Ambystoma tigrinum virus (ATV) (family Iridoviridae, genus Ranavirus) was isolated from diseased tiger salamanders (Ambystoma tigrinum stebbinsi) from the San Rafael Valley in southern Arizona, USA in 1996. Genomic sequencing of ATV, as well as other members of the genus, identified an open reading frame that has homology to the eukaryotic translation initiation factor, eIF2α (ATV eIF2α homologue, vIF2αH). Therefore, we asked if the ATV vIF2αH could also inhibit PKR. To test this hypothesis, the ATV vIF2αH was cloned into vaccinia virus (VACV) in place of the well-characterized VACV PKR inhibitor, E3L...
September 14, 2017: Virology
https://www.readbyqxmd.com/read/28918322/time-for-tiger-to-roar-technology-informatics-guiding-education-reform
#4
EDITORIAL
Siobhan O'Connor, Ursula Hubner, Toria Shaw, Rachelle Blake, Marion Ball
Information Technology (IT) continues to evolve and develop with electronic devices and systems becoming integral to healthcare in every country. This has led to an urgent need for all professions working in healthcare to be knowledgeable and skilled in informatics. The Technology Informatics Guiding Education Reform (TIGER) Initiative was established in 2006 in the United States to develop key areas of informatics in nursing. One of these was to integrate informatics competencies into nursing curricula and life-long learning...
August 19, 2017: Nurse Education Today
https://www.readbyqxmd.com/read/28903996/crouching-tiger-hidden-dragon
#5
Daniel Hopkins, Murali Varma
No abstract text is available yet for this article.
September 13, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28899645/transcriptome-based-discovery-of-pathways-and-genes-related-to-reproduction-of-the-black-tiger-shrimp-penaeus-monodon
#6
Tanaporn Uengwetwanit, Pattareeya Ponza, Duangjai Sangsrakru, Duangdao Wichadakul, Supawadee Ingsriswang, Rungnapa Leelatanawit, Sirawut Klinbunga, Sithichoke Tangphatsornruang, Nitsara Karoonuthaisiri
The black tiger shrimp (Penaeus monodon) is an aquatic animal with considerable economic importance. Poor reproductive maturation in captivity impedes sustainable aquaculture production of this species. This study aims to provide transcriptomic information on reproductive organs using 454 pyrosequencing technology. The transcriptome analysis of ovaries and testes revealed 41,136 transcripts with 20,192 contigs. We found novel sets of transcripts completing several important reproductive pathways such as gonadotropin-releasing hormone (GnRH) signaling and progesterone-mediated oocyte maturation...
September 9, 2017: Marine Genomics
https://www.readbyqxmd.com/read/28882801/effect-of-immune-gene-silencing-in-wssv-infected-tiger-shrimp-penaeus-monodon
#7
M S Shekhar, A Gomathi, N K Dubey, K Vinaya Kumar, K K Vijayan
White spot syndrome virus, continues to cause huge economic loss to aquaculture industry. In the absence of effective therapeutics to control WSSV, it is important to understand the host pathogen interaction at the molecular level. Suppression subtractive hybridization (SSH) cDNA library was constructed which led to identification of several differentially expressed genes in response to WSSV infection in Penaeus monodon. The genes expressed in SSH cDNA library of shrimp gill and gut tissues belonged to a wide range of biological functions...
September 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28882278/urban-street-structure-and-traffic-safety
#8
Dinesh Mohan, Shrikant I Bangdiwala, Andres Villaveces
INTRODUCTION: This paper reports the influence of road type and junction density on road traffic fatality rates in U.S. cities. METHOD: The Fatality Analysis Reporting System (FARS) files were used to obtain fatality rates for all cities for the years 2005-2010. A stratified random sample of 16 U.S. cities was taken, and cities with high and low road traffic fatality rates were compared on their road layout details (TIGER maps were used). Statistical analysis was done to determine the effect of junction density and road type on road traffic fatality rates...
September 2017: Journal of Safety Research
https://www.readbyqxmd.com/read/28881514/-phenotypic-and-genotypic-features-of-twenty-children-with-classic-pantothenate-kinase-associated-neurodegeneration
#9
J Zhou, J He, L P Kou, H C Feng, Y H Deng, Z B Zhang, L Zhou, J M Wang, Y W Jiang, Y Wu
Objective: To explore the phenotypic and genotypic characteristics in Chinese children with classic pantothenate kinase-associated neurodegeneration (PKAN). Method: The clinical, radiographic and genetic data of all PKAN patients diagnosed at pediatric department of Peking University First Hospital from November 2006 to December 2016 were retrospectively collected and analyzed. Result: Twenty patients with classic PKAN were included in the study. The median age at onset was 3.5 years (ranging from 1.0 to 10...
September 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28877579/prevalence-of-centrocestus-formosanus-metacercariae-in-ornamental-fish-from-chiang-mai-thailand-with-molecular-approach-using-its2
#10
Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong-Yil Chai
The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C...
August 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28877511/severe-neurotoxicity-requiring-mechanical-ventilation-in-a-dog-envenomed-by-a-red-bellied-black-snake-pseudechis-porphyriacus-and-successful-treatment-with-an-experimental-bivalent-whole-equine-igg-antivenom
#11
A M Padula, E M Leister
Snakebite in dogs from Pseudechis porphyriacus (red-bellied black snake; RBBS) is a common envenomation treated by veterinarians in Australia where this snake occurs. This case report describes the successful treatment of a clinically severe RBBS envenomation in a dog with an experimental bivalent equine whole IgG antivenom and mechanical ventilation, following its presentation in a cyanotic state. The cause of the cyanosis and respiratory distress was considered due to paralysis from neurotoxins in RBBS venom...
September 4, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28873118/topical-timolol-may-improve-overall-scar-cosmesis-in-acute-surgical-wounds
#12
Ganary Dabiri, Jeffrey Tiger, Renato Goreshi, Allison Fischer, Satori Iwamoto
Acute wounds created by dermatologists following the removal of nonmelanoma skin cancers are closed either by primary or secondary intention, and the best cosmetic outcome is preferentially desired. One parameter that determines the overall cosmesis of the healed wound is its vascularity. Vascular tone results from a complex interplay of a variety of chemokines in the body and their interaction with receptors located on endothelial cell surfaces. In this study, our aim was to determine if topical timolol could improve the overall cosmesis of acute surgical wounds...
July 2017: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/28872900/enceladus-plume-structure-and-time-variability-comparison-of-cassini-observations
#13
Ben D Teolis, Mark E Perry, Candice J Hansen, J Hunter Waite, Carolyn C Porco, John R Spencer, Carly J A Howett
During three low-altitude (99, 66, 66 km) flybys through the Enceladus plume in 2010 and 2011, Cassini's ion neutral mass spectrometer (INMS) made its first high spatial resolution measurements of the plume's gas density and distribution, detecting in situ the individual gas jets within the broad plume. Since those flybys, more detailed Imaging Science Subsystem (ISS) imaging observations of the plume's icy component have been reported, which constrain the locations and orientations of the numerous gas/grain jets...
September 2017: Astrobiology
https://www.readbyqxmd.com/read/28867209/identification-and-molecular-profiling-of-dc-sign-like-from-big-belly-seahorse-hippocampus-abdominalis-inferring-its-potential-relevancy-in-host-immunity
#14
Eunyoung Jo, Don Anushka Sandaruwan Elvitigala, Qiang Wan, Minyoung Oh, Chulhong Oh, Jehee Lee
Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippocampus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52...
September 1, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28864704/-it-is-merely-a-paper-tiger-battle-for-increased-tobacco-advertising-regulation-in-indonesia-content-analysis-of-news-articles
#15
Putu Ayu Swandewi Astuti, Becky Freeman
OBJECTIVE: At the end of 2012, the Indonesian government enacted tobacco control regulation (PP 109/2012) that included stricter tobacco advertising, promotion and sponsorship (TAPS) controls. The PP did not ban all forms of TAPS and generated a great deal of media interest from both supporters and detractors. This study aims to analyse stakeholder arguments regarding the adoption and implementation of the regulation as presented through news media converge. DESIGN: Content analysis of 213 news articles reporting on TAPS and the PP that were available from the Factiva database and the Google News search engine...
September 1, 2017: BMJ Open
https://www.readbyqxmd.com/read/28855292/extracellular-h-fluxes-from-tiger-salamander-m%C3%A3-ller-glial-cells-measured-using-self-referencing-h-selective-microelectrodes
#16
Matthew A Kreitzer, David Swygart, Meredith Osborn, Blair Skinner, Chad Heer, Ryan Kaufman, Bethany Williams, Lexi Shepherd, Hannah Caringal, Michael Gongwer, Boriana K Tchernookova, Robert Paul Malchow
Self-referencing H+-selective electrodes were used to measure extracellular H+ fluxes from Müller (glial) cells isolated from the tiger salamander retina. A novel chamber enabled stable recordings using H+-selective microelectrodes in a self-referencing format using bicarbonate-based buffer solutions. A small basal H+ flux was observed from the end-foot region of quiescent cells bathed in 24 mM bicarbonate-based solutions, and increasing extracellular potassium induced a dose-dependent increase in H+ flux...
August 30, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28852506/energy-metabolism-in-mobile-wild-sampled-sharks-inferred-by-plasma-lipids
#17
Austin J Gallagher, Rachel A Skubel, Heidi R Pethybridge, Neil Hammerschlag
Evaluating how predators metabolize energy is increasingly useful for conservation physiology, as it can provide information on their current nutritional condition. However, obtaining metabolic information from mobile marine predators is inherently challenging owing to their relative rarity, cryptic nature and often wide-ranging underwater movements. Here, we investigate aspects of energy metabolism in four free-ranging shark species (n = 281; blacktip, bull, nurse, and tiger) by measuring three metabolic parameters [plasma triglycerides (TAG), free fatty acids (FFA) and cholesterol (CHOL)] via non-lethal biopsy sampling...
2017: Conservation Physiology
https://www.readbyqxmd.com/read/28851952/conservation-priorities-for-endangered-indian-tigers-through-a-genomic-lens
#18
Meghana Natesh, Goutham Atla, Parag Nigam, Yadvendradev V Jhala, Arun Zachariah, Udayan Borthakur, Uma Ramakrishnan
Tigers have lost 93% of their historical range worldwide. India plays a vital role in the conservation of tigers since nearly 60% of all wild tigers are currently found here. However, as protected areas are small (<300 km(2) on average), with only a few individuals in each, many of them may not be independently viable. It is thus important to identify and conserve genetically connected populations, as well as to maintain connectivity within them. We collected samples from wild tigers (Panthera tigris tigris) across India and used genome-wide SNPs to infer genetic connectivity...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28845770/tiger-s-milk-medicinal-mushroom-lignosus-rhinocerotis-agaricomycetes-sclerotium-inhibits-nitric-oxide-production-in-lps-stimulated-bv2-microglia
#19
Syntyche Ling-Sing Seow, Murali Naidu, Vikineswary Sabaratnam, Sharmili Vidyadaran, Kah-Hui Wong
In Malaysia and China, the sclerotium of Lignosus rhinocerotis is used by local communities and traditional medicine practitioners as a general tonic and remedy to treat a variety of ailments, including inflammation-associated disorders. In this study, 10 samples from different preparations of L. rhinocerotis sclerotium, including a hot aqueous extract (HAE), an ethanol extract (EE), fractions from the HAE and EE, and crude polysaccharides, were tested for their in vitro cytotoxic and nitric oxide (NO) inhibitory activities in lipopolysaccharide (LPS)--stimulated BV2 microglia...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28844332/the-ambystoma-tigrinum-virus-atv-rnase-iii-gene-can-modulate-host-pkr-activation-and-interferon-production
#20
Alexander G Allen, Scott Morgans, Eric Smith, Mariah M Aron, James K Jancovich
The iridovirus RNase III gene is one of 26 conserved core genes among the family Iridoviridae. Initial studies suggest this viral protein functions to suppress RNA interference pathways that may attack viral RNA during infection. Therefore, to determine if the Ambystoma tigrinum virus (ATV) RNase III-like gene (ORF 25R) can modulate the host innate immune response fish and human cells ectopically expressing 25R were treated with polyI:C and monitored for interferon synthesis and phosphorylation of eIF2α and PKR...
August 22, 2017: Virology
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