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Emma Popo, Sara Kenyon, Sophie-Anna Dann, Christine MacArthur, Jacqueline Blissett
BACKGROUND: The ELSIPS (Evaluation of Lay Support in Pregnant Women with Social Risk) RCT showed that lay support for women with social risk had a positive effect on maternal mental health and mother-infant bonding. This exploratory study examined whether these observed benefits would impact infant development at 1 year. METHODS: A sub-sample of women whose infants were under one year who had participated in the ELSIPS RCT which randomised women to receive either standard care or the services of a Pregnancy Outreach Worker (POW), and who were contactable, were eligible to participate in the follow up...
2017: PloS One
April Maschmann, Kristy L Kounovsky-Shafer
Optical mapping, a single DNA molecule genome analysis platform that can determine methylation profiles, uses fluorescently labeled DNA molecules that are elongated on the surface and digested with a restriction enzyme to produce a barcode of that molecule. Understanding how the cyanine fluorochromes affect enzyme activity can lead to other fluorochromes used in the optical mapping system. The effects of restriction digestion on fluorochrome labeled DNA (Ethidium Bromide, DAPI, H33258, EthD-1, TOTO-1) have been analyzed previously...
June 3, 2017: Nucleosides, Nucleotides & Nucleic Acids
Nonhlanhla R Dlamini, Popo Maja
During the past two decades, immunisation has saved millions of lives and prevented countless illnesses and disabilities in South Africa(SA). However, vaccine-preventable diseases are still a threat. A vaccine-preventable disease that might lead to a 1- or 2-week illness in anadult, could prove deadly for infants, children or elderly people. Vaccination protects oneself and one's family. For example, adults are themost common source of pertussis (whooping cough) infection in infants, which can be deadly for the latter...
June 17, 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
Andreas S Biebricher, Iddo Heller, Roel F H Roijmans, Tjalle P Hoekstra, Erwin J G Peterman, Gijs J L Wuite
DNA intercalators are widely used as fluorescent probes to visualize DNA and DNA transactions in vivo and in vitro. It is well known that they perturb DNA structure and stability, which can in turn influence DNA-processing by proteins. Here we elucidate this perturbation by combining single-dye fluorescence microscopy with force spectroscopy and measuring the kinetics of DNA intercalation by the mono- and bis-intercalating cyanine dyes SYTOX Orange, SYTOX Green, SYBR Gold, YO-PRO-1, YOYO-1 and POPO-3. We show that their DNA-binding affinity is mainly governed by a strongly tension-dependent dissociation rate...
2015: Nature Communications
T A Kouévi, B van Mierlo, C Leeuwis
This article discusses the extent to which a responsive evaluation (RE) approach contributed to learning by stakeholders in a case of high complexity. Fishery management in Grand-Popo, Benin is characterized by ambiguity, that is contrasting views among fishery stakeholders about what should be done, why, how, where, and when to resolve fishery problems like the depletion of fish-stock and absence of income alternatives. It was also characterized by great gaps (mismatches) between interventionists' plans and actions, despite generations of interventions and evaluations of their effectiveness...
December 2013: Evaluation and Program Planning
Christian F Kammerer, Jörg Fröbisch, Kenneth D Angielczyk
The large dicynodont Eubrachiosaurus browni from the Upper Triassic Popo Agie Formation of Wyoming is redescribed. Eubrachiosaurus is a valid taxon that differs from Placerias hesternus, with which it was previously synonymized, by greater anteroposterior expansion of the scapula dorsally and a very large, nearly rectangular humeral ectepicondyle with a broad supinator process. Inclusion of Eubrachiosaurus in a revised phylogenetic analysis of anomodont therapsids indicates that it is a stahleckeriid closely related to the South American genera Ischigualastia and Jachaleria...
2013: PloS One
Alexander Zarkov, Aleksey Vasilev, Todor Deligeorgiev, Stoyno Stoynov, Marina Nedelcheva-Veleva
To answer the demands of scientific and medical imaging issues, the family of nucleic acid fluorescent dyes is constantly enlarging. Most of the developed dyes reveal high qualities in bulk solution assays but are inefficient to produce a strong and sufficiently stable signal to enable the application of single-molecule techniques. Therefore, we tested 12 novel monomeric and homodimeric cyanine dyes for potential single DNA molecule imaging. Although their qualities in bulk solutions have already been described, nothing was known about their behavior on a single-molecule level...
March 2013: Molecular Imaging
S Winter, G Loeber
No abstract text is available yet for this article.
January 1997: Journal of Biomedical Optics
A T Kouévi, B Van Mierlo, C Leeuwis, S D Vodouhê
The main question addressed by this article is how to adapt the responsive evaluation (RE) approach to an intervention context characterized by repetition of ineffective interventions, ambiguous intervention action theories among stakeholders, and high complexity. The context is Grand-Popo, a fishing municipality located on Benin's southwest Atlantic coast. The fishery management interventionists and the fishing communities in the municipality all espoused concern for the sustainable improvement of fishing actors' livelihood conditions, but differed about the reasons for this livelihood impairment, and about what should be done, when, where, and by whom...
February 2013: Evaluation and Program Planning
Yi-Tui Chen, Chung-Chiang Chen
This paper attempts to compare the relative efficiency across MSW incineration facilities under the three operating modes including government-owned government-operated (GOO), government-owned private-operated (GOPO), and private-owned private-operated (POPO) by using the Data Envelopment Analysis (DEA), and to examine the factor in affecting the efficiency variation. The results show that technical efficiency scores are 0.7538, 0.9046 and 0.9917 for GOO, GOPO and POPO, respectively. An ANOVA is conducted and the analysis result confirms that the operating mode has significant impact on technical efficiencies...
March 2012: Waste Management
Xiao Zhang, Pei Tao
OBJECTIVE: To test the effects of different surface treatments on the adhesive strength between POPO fiber post and resin cement. METHODS: 50 fiber posts were divided into 5 groups at random according to different surface treatments. Group A: Control group, no treatment. Group B: Silanization for 60 s. Group C: Etching for 30 s. Group D: Etching for 60 s. Group E: Etching for 120 s. Crowns of 45 fresh premolars were removed and assigned randomly to 5 test groups...
October 2011: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
A Van Orden, H Cai, P M Goodwin, R A Keller
This paper reports the demonstration of efficient single molecule detection in flow cytometry by two-photon fluorescence excitation. We have used two-photon excitation (TPE) to detect single DNA fragments as small as 383 base pairs (bp) labeled with the intercalating dye, POPO-1, at a dye:nucleotide ratio of 1:5. TPE of the dye-DNA complexes was accomplished using a mode-locked, 120 fs pulse width Ti:sapphire laser operating at 810 nm. POPO-1 labeled DNA fragments of 1.1 kilobase pairs (kbp) and larger were sequentially detected in our flow cytometry system with a detection efficiency of nearly 100%...
June 1, 1999: Analytical Chemistry
Xiao Zhang, Jin-Le Li, Liang Hao
OBJECTIVE: To investigate the correlation of morphological characteristics and failure modes of two types of glass-fiber posts luted with self-adhesive resin cement and etch-and-rinse adhesive system. METHODS: Thirty-six intact single-rooted premolars were collected and removed the crown. After root canal therapy, teeth were randomly divided into 4 groups. Group A: Self-adhesive with POPO fiber post. Group B: Etch-and-rinse with POPO fiber posts. Group C: Self-adhesive with Para Post...
April 2011: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Huichen Guo, Niagara M Idris, Yong Zhang
In this work, we demonstrate near-infrared-to-visible upconverting fluorescent nanoparticles as a promising platform for lanthanide-based or luminescence resonance energy transfer (LRET)-based biodetection of DNA release in live cells. Highly monodispersed, stable aqueous suspension of nanoparticles, surface-functionalized with amino groups for binding to DNA, were prepared and characterized. These amino-functionalized nanoparticles were able to electrostatically bind to DNA and protect it from DNaseI degradation as shown by gel electrophoresis...
March 15, 2011: Langmuir: the ACS Journal of Surfaces and Colloids
Bijan Kumar Dey, Xiao-Li Zhao, Emmanuel Popo-Ola, Ana Regina Campos
The Drosophila disconnected (disco) gene encodes a C(2)H(2)-type zinc finger transcription factor required for the development of the central and peripheral nervous systems. We report that disco participates in a positive feedback loop with the Dll gene, a master regulator of ventral appendage development. Dll function is not only required for proper disco expression in antenna and leg discs, but is also sufficient for ectopic expression of disco in the developing retina and wing imaginal discs. Conversely, disco gene function is required for the maintenance of Dll expression...
November 2009: Cell and Tissue Research
W D Keller
No abstract text is available yet for this article.
February 29, 1952: Science
(no author information available yet)
The photograph on page 1415 accompanying the book review of Luna B. Leopold's A View of the River by Vic Baker (25 Nov., p. 1414) showed meanders of the East Fork River near Boulder, Wyoming, not the Popo Agie River.
January 27, 1995: Science
K P Hennes, C A Suttle, A M Chan
Fluorescently stained viruses were used as probes to label, identify, and enumerate specific strains of bacteria and cyanobacteria in mixed microbial assemblages. Several marine virus isolates were fluorescently stained with YOYO-1 or POPO-1 (Molecular Probes, Inc.) and added to seawater samples that contained natural microbial communities. Cells to which the stained viruses adsorbed were easily distinguished from nonhost cells; typically, there was undetectable binding of stained viruses to natural microbial assemblages containing >10(sup6) bacteria ml(sup-1) but to which host cells were not added...
October 1995: Applied and Environmental Microbiology
Victor M Gómez-Muñoz, Miguel Angel Porta-Gándara, Jaime Cervantes de Gortari
A simple Bayesian method to estimate proportional payments for using or constructing a wastewater treatment plant is presented. The method addresses the usually unknown individual participation in global pollution, of municipal and industrial wastewaters that are normally discharged into the same collecting pond. The individual contributions are calculated from the discharged volume of each source and its pollution percentage in the wastewater volume previous to the disposal. According to the method presented here, the costs in building and/or running the plant can be covered by proportional individual payments based on the individual contributions, in a more even way...
January 2006: Water Research
José R Sotelo-Silveira, Aldo Calliari, Magdalena Cárdenas, Edward Koenig, José R Sotelo
Periaxoplasmic ribosomal plaques (PARPs) are discrete ribosome-containing domains distributed intermittently along the periphery of axoplasm in myelinated fibers. Thus, they are structural formations in which translational machinery is spatially organized to serve as centers of protein synthesis for local metabolic requirements and perhaps repair as well. Because of evidence that RNA is transported to putative PARP domains, involving both microtubule- and actin-based mechanisms, it was of interest to investigate whether cytoskeletal motor proteins exhibit a nonrandom localization within PARP domains...
August 2004: Journal of Neurobiology
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