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https://www.readbyqxmd.com/read/29440573/the-colm-family-polymorphic-toxins-breaching-the-bacterial-cell-wall
#1
Maarten G K Ghequire, Susan K Buchanan, René De Mot
Bacteria host an arsenal of antagonism-mediating molecules to combat for ecologic space. Bacteriocins represent a pivotal group of secreted antibacterial peptides and proteins assisting in this fight, mainly eliminating relatives. Colicin M, a model for peptidoglycan-interfering bacteriocins in Gram-negative bacteria, appears to be part of a set of polymorphic toxins equipped with such a catalytic domain (ColM) targeting lipid II. Diversifying recombination has enabled parasitism of different receptors and has also given rise to hybrid bacteriocins in which ColM is associated with another toxin module...
February 13, 2018: MBio
https://www.readbyqxmd.com/read/29414908/the-reaction-of-oxy-hemoglobin-with-nitrite-mechanism-antioxidant-modulated-effect-and-implications-for-blood-substitute-evaluation
#2
Denisa Hathazi, Florina Scurtu, Cristina Bischin, Augustin Mot, Amr A A Attia, Jacob Kongsted, Radu Silaghi-Dumitrescu
The autocatalytic reaction between nitrite and the oxy form of globins involves free radicals. For myoglobin (Mb), an initial binding of nitrite to the iron-coordinated oxygen molecule was proposed; the resulting ferrous-peroxynitrate species was not detected, but its decay product, the high-valent ferryl form, was demonstrated in stopped-flow experiments. Reported here are the stopped flow spectra recorded upon mixing oxy Hb (native, as well as chemically-derivatized in the form of several candidates of blood substitutes) with a supraphysiological concentration of nitrite...
February 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29401813/single-shot-molecular-orbital-tomography-with-orthogonal-two-color-fields
#3
Chunyang Zhai, Xiaofan Zhang, Xiaosong Zhu, Lixin He, Yinfu Zhang, Baoning Wang, Qingbin Zhang, Pengfei Lan, Peixiang Lu
Molecular orbital tomography (MOT) based on high-order-harmonic generation opens a way to track the molecular electron dynamics or even follow a chemical reaction. However, the real-time imaging of the evolution of electron orbitals is hampered by the multi-shot measurement of high-order harmonics. Here, we report a single-shot MOT scheme with orthogonal two-color (OTC) fields. This scheme enables the tomographic imaging of molecular orbital with single-shot measurement in experiment, owing to the two-dimensional manipulation of the electron motion in OTC fields...
February 5, 2018: Optics Express
https://www.readbyqxmd.com/read/29399315/evaluation-of-200-mm-365-mm-and-500-mm-fibers-of-ho-yag-laser-in-transurethral-lithotripsy-of-ureteral-a-randomize-control-trial
#4
Morteza Fallah Karkan, Saleh Ghiasy, Arash Ranjbar, Babak Javanmard
Introduction: Presently, different holmium: yttrium aluminum garnet (Ho:YAG) laser calibers are used for endoscopic stone treatment, which include 200, 365, 500 and 1000 Mm fibers. Currently, there are not enough studies to compare the performance of these fibers. In this retrospective investigation, we compared the outcome of 200, 365 and 500 Mm fibers of Ho:YAG laser in transurethral lithotripsy of ureteral stone. Methods: From January 2016 to June 2017, 74 subjects with mean age of 35.3 ± 5.6 were randomly allocated to 3 groups according to the caliber of laser, 200, 365 and 500 Mm for transurethral lithotripsy...
2018: Journal of Lasers in Medical Sciences
https://www.readbyqxmd.com/read/29383768/effects-of-the-supplementation-with-an-high-polyphenols-extra-virgin-olive-oil-on-kinetic-sperm-features-and-seminal-plasma-oxidative-status-in-healthy-dogs
#5
V Tufarelli, A Rizzo, G M Lacalandra, A C Guaricci, V Laudadio, L Valentini
The aim of this work was to evaluate the effects of the supplementation of two extra-virgin olive oils (EVOO) having different polyphenols content, on canine spermatozoa kinetic parameters and seminal plasma oxidative status. The study was conducted on 12 clinically healthy dogs of different breeds (2-7 years, 5-48 kg of body weight) divided into two groups: an experimental group supplemented with EVOO (Coratina cultivar) high in polyphenols (H-P) and a control group fed EVOO (Cima di Bitonto cultivar) low in polyphenols (L-P)...
January 30, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29382740/a-plastidial-pantoate-transporter-with-a-potential-role-in-pantothenate-synthesis
#6
Lili Huang, Michal Pyc, Saleh Alseekh, Donald R McCarty, Valérie de Crécy-Lagard, Jesse F Gregory, Christopher S Henry, Alisdair R Fernie, Robert T Mullen, Andrew D Hanson
The pantothenate (vitamin B5) synthesis pathway in plants is not fully defined because the sub-cell-ular site of its ketopantoate → pantoate reduction step is unclear. However, the pathway is known to be split between cytosol, mit-o-chondria, and potentially plastids, and inferred to involve mitochondrial or plast-idial transport of ketopantoate or pantoate. No proteins that mediate these transport steps have been identified. Compar-at-ive genomic and transcriptomic analyses identified Arabidopsis thal-iana BASS1 (At1g78560) and its maize ( Zea mays ) ortholog as candidates for such a transport role...
January 30, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29379547/persistence-of-primitive-reflexes-and-associated-motor-problems-in-healthy-preschool-children
#7
Ewa Z Gieysztor, Anna M Choińska, Małgorzata Paprocka-Borowicz
Introduction: Retained primitive reflexes can disturb natural development and involve difficulties in social and educational children's life. They can also impact on psychomotor development. Mature responses in a child's psychomotor progress can only occur if the central nervous system itself has reached maturity. The process consist the transition made from brain stem reflex response to cortically controlled response. This study define the occurrence of primitive reflexes in healthy 4-6 years old children and analyze the impact of survived primitive reflexes on psychomotor development...
January 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29358635/%C3%AE-enhanced-grey-molasses-on-the-d2-transition-of-rubidium-87-atoms
#8
Sara Rosi, Alessia Burchianti, Stefano Conclave, Devang S Naik, Giacomo Roati, Chiara Fort, Francesco Minardi
Laser cooling based on dark states, i.e. states decoupled from light, has proven to be effective to increase the phase-space density of cold trapped atoms. Dark-states cooling requires open atomic transitions, in contrast to the ordinary laser cooling used for example in magneto-optical traps (MOTs), which operate on closed atomic transitions. For alkali atoms, dark-states cooling is therefore commonly operated on the D1 transition nS1/2 → nP1/2. We show that, for 87Rb, thanks to the large hyperfine structure separations the use of this transition is not strictly necessary and that "quasi-dark state" cooling is efficient also on the D2 line, 5S1/2 → 5P3/2...
January 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29340644/proteasome-inhibition-increases-the-efficiency-of-lentiviral-vector-mediated-transduction-of-trabecular-meshwork
#9
Zeynep Aktas, Hongyu Rao, Sarah R Slauson, B'Ann T Gabelt, Inna V Larsen, Rachael T C Sheridan, Leonie Herrnberger, Ernst R Tamm, Paul L Kaufman, Curtis R Brandt
Purpose: To determine if proteasome inhibition using MG132 increased the efficiency of FIV vector-mediated transduction in human trabecular meshwork (TM)-1 cells and monkey organ-cultured anterior segments (MOCAS). Methods: TM-1 cells were pretreated for 1 hour with 0.5% dimethyl sulfoxide (DMSO; vehicle control) or 5 to 50 μM MG132 and transduced with FIV.GFP (green fluorescent protein)- or FIV.mCherry-expressing vector at a multiplicity of transduction (MOT) of 20...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29333611/cognitive-dysfunction-after-generalized-tonic-clonic-status-epilepticus-in-adults
#10
K N Power, A Gramstad, N E Gilhus, K O Hufthammer, B A Engelsen
OBJECTIVES: Generalized tonic-clonic status epilepticus (GTC-SE) is considered a risk for cognitive impairment. Research with standardized tools is scarce and non-conclusive. We systematically assessed short-term and long-term cognitive function after GTC-SE. MATERIALS AND METHODS: Thirty-three patients were tested after the clinical post-ictal phase of GTC-SE (timepoint 1) and again after 1 year (timepoint 2). Twenty controls were examined with the same tests...
January 14, 2018: Acta Neurologica Scandinavica
https://www.readbyqxmd.com/read/29316649/%C3%A2-cat-3-mots-nanosatellite-mission-for-optical-multispectral-and-gnss-r-earth-observation-concept-and-analysis
#11
Jordi Castellví, Adriano Camps, Jordi Corbera, Ramon Alamús
The ³Cat-3/MOTS (3: Cube, Cat: Catalunya, 3: 3rd CubeSat mission/Missió Observació Terra Satèl·lit) mission is a joint initiative between the Institut Cartogràfic i Geològic de Catalunya (ICGC) and the Universitat Politècnica de Catalunya-BarcelonaTech (UPC) to foster innovative Earth Observation (EO) techniques based on data fusion of Global Navigation Satellite Systems Reflectometry (GNSS-R) and optical payloads. It is based on a 6U CubeSat platform, roughly a 10 cm × 20 cm × 30 cm parallelepiped...
January 6, 2018: Sensors
https://www.readbyqxmd.com/read/29314727/molecular-processes-underlying-synergistic-linuron-mineralization-in-a-triple-species-bacterial-consortium-biofilm-revealed-by-differential-transcriptomics
#12
Pieter Albers, Bram Weytjens, René De Mot, Kathleen Marchal, Dirk Springael
The proteobacteria Variovorax sp. WDL1, Comamonas testosteroni WDL7, and Hyphomicrobium sulfonivorans WDL6 compose a triple-species consortium that synergistically degrades and grows on the phenylurea herbicide linuron. To acquire a better insight into the interactions between the consortium members and the underlying molecular mechanisms, we compared the transcriptomes of the key biodegrading strains WDL7 and WDL1 grown as biofilms in either isolation or consortium conditions by differential RNAseq analysis...
January 3, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29286768/steady-state-magneto-optical-trap-with-100-fold-improved-phase-space-density
#13
Shayne Bennetts, Chun-Chia Chen, Benjamin Pasquiou, Florian Schreck
We demonstrate a continuously loaded ^{88}Sr magneto-optical trap (MOT) with a steady-state phase-space density of 1.3(2)×10^{-3}. This is 2 orders of magnitude higher than reported in previous steady-state MOTs. Our approach is to flow atoms through a series of spatially separated laser cooling stages before capturing them in a MOT operated on the 7.4-kHz linewidth Sr intercombination line using a hybrid slower+MOT configuration. We also demonstrate producing a Bose-Einstein condensate at the MOT location, despite the presence of laser cooling light on resonance with the 30-MHz linewidth transition used to initially slow atoms in a separate chamber...
December 1, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29284999/high-burden-of-stroke-risk-factors-in-developing-country-the-case-study-of-bosnia-herzegovina
#14
Marija Bender, Edin Jusufovic, Vesna Railic, Sima Kelava, Selma Tinjak, Damir Dzevdetbegovic, Dario Mot, Mensuda Tresnjo, Sandra Lakicevic, Natasa Pejanovic-Skobic, Osman Sinanovic
Introduction: The burden of stroke has been increasing worldwide, especially in developing countries. Very few data regarding epidemiology of stroke are available in Bosnia and Herzegovina (BH). Patients and methods: We undertook a retrospective hospital-based study in all hospitals existing in five cantons and one district of BH. The patients were recruited between January 1st, 2014, and December 31st, 2014, and only first-ever-in-lifetime strokes (FES) were included for evaluation...
December 2017: Materia Socio-medica
https://www.readbyqxmd.com/read/29251597/activation-of-the-dopaminergic-pathway-from-vta-to-the-medial-olfactory-tubercle-generates-odor-preference-and-reward
#15
Zhijian Zhang, Qing Liu, Pengjie Wen, Jiaozhen Zhang, Xiaoping Rao, Ziming Zhou, Hongruo Zhang, Xiaobin He, Juan Li, Zheng Zhou, Xiaoran Xu, Xueyi Zhang, Rui Luo, Guanghui Lv, Haohong Li, Pei Cao, Liping Wang, Fuqiang Xu
Odor-preferences are usually influenced by life experiences. However, the neural circuit mechanisms remain unclear. The medial olfactory tubercle (mOT) is involved in both reward and olfaction, while the ventral tegmental area (VTA) dopaminergic (DAergic) neurons are considered to be engaged in reward and motivation. Here, we found that the VTA (DAergic)-mOT pathway could be activated by different types of naturalistic rewards as well as odors in DAT-cre mice. Optogenetic activation of the VTA-mOT DAergic fibers was able to elicit preferences for space, location and neutral odor, while pharmacological blockade of the dopamine receptors in the mOT fully prevented the odor-preference formation...
December 18, 2017: ELife
https://www.readbyqxmd.com/read/29244875/effect-of-age-and-stereopsis-on-a-multiple-object-tracking-task
#16
Marjolaine Plourde, Marie-Eve Corbeil, Jocelyn Faubert
3D vision develops during childhood and tends to diminish after 65 years of age. It is still relatively unknown how stereopsis is used in more complex/ecological contexts such as when walking about in crowds where objects are in motion and occlusions occur. One task that shares characteristics with the requirements for processing dynamic crowds is the multiple object-tracking task (MOT). In the present study we evaluated the impact of stereopsis on a MOT task as a function of age. A total of 60 observers consisting of three groups of 20 subjects (7-12 years old, 18-40 years old and 65 years and older) completed the task in both conditions (with and without stereoscopic effects)...
2017: PloS One
https://www.readbyqxmd.com/read/29242099/downregulation-of-circulating-mots-c-levels-in-patients-with-coronary-endothelial-dysfunction
#17
Qing Qin, Silvia Delrio, Junxiang Wan, R Jay Widmer, Pinchas Cohen, Lilach O Lerman, Amir Lerman
BACKGROUND: MOTS-c is one of the newly identified mitochondrial-derived peptides which play a role in regulating metabolic homeostasis. The current study aimed to investigate whether circulating MOTS-c levels are also associated with endothelial dysfunction(ED) in patients without significant structural coronary lesions. METHODS: Forty patients undergoing coronary angiography and endothelial function testing for clinical indications of recurrent angina with no structural coronary lesions were included in the study...
December 6, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/29238912/hue-distinctiveness-overrides-category-in-determining-performance-in-multiple-object-tracking
#18
Mengdan Sun, Xuemin Zhang, Lingxia Fan, Luming Hu
The visual distinctiveness between targets and distractors can significantly facilitate performance in multiple object tracking (MOT), in which color is a feature that has been commonly used. However, the processing of color can be more than "visual." Color is continuous in chromaticity, while it is commonly grouped into discrete categories (e.g., red, green). Evidence from color perception suggested that color categories may have a unique role in visual tasks independent of its chromatic appearance. Previous MOT studies have not examined the effect of chromatic and categorical distinctiveness on tracking separately...
December 13, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/29227799/cognitive-function-after-status-epilepticus-versus-after-multiple-generalized-tonic-clonic-seizures
#19
Kjersti N Power, Arne Gramstad, Nils Erik Gilhus, Karl Ove Hufthammer, Bernt A Engelsen
OBJECTIVES: Status epilepticus (SE) is considered a risk for cognitive impairment. Studies have indicated that SE cause more cognitive decline than multiple lifetime generalized tonic clonic (GTC) seizures. The aim of the study was to investigate whether patients suffering from SE or from multiple lifetime GTC seizures have cognitive dysfunction, and if the disabilities differ between these groups. MATERIALS AND METHODS: Patients suffering from SE were evaluated shortly after the clinical post-ictal phase and again after one year...
November 28, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/29212086/comparative-analyses-of-transport-proteins-encoded-within-the-genomes-of-bdellovibrio-bacteriovorus-hd100-and-bdellovibrio-exovorus-jss
#20
Fereshteh Heidari Tajabadi, Arturo Medrano-Soto, Masoud Ahmadzadeh, Gholamreza Salehi Jouzani, Milton H Saier
Bdellovibrio, δ-proteobacteria, including B. bacteriovorus (Bba) and B. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and drug efflux systems, and 3 times more lipid transporters...
December 7, 2017: Journal of Molecular Microbiology and Biotechnology
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