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https://www.readbyqxmd.com/read/29928975/the-high-affinity-of-small-molecule-antioxidants-for-hemoglobin
#1
Cristina Puscas, Luana Radu, Francisco Carrascoza, Augustin C Mot, Diana Amariei, Oana Lungu, Florina Scurtu, Paula Podea, Raluca Septelean, Alina Matei, Mihaela Mic, Amr A Attia, Radu Silaghi-Dumitrescu
Hemoglobin has previously been shown to display ascorbate peroxidase and urate peroxidase activity, with measurable Michaelis-Menten parameters that reveal a particularly low Km for ascorbate as well as for urate - lower than the respective in vivo concentrations of these antioxidants in blood. Also, direct detection of a hemoglobin-ascorbate interaction was possible by monitoring the 1H-NMR spectrum of ascorbate in the presence of hemoglobin. The relative difference in structures between ascorbate and urate may raise the question as to exactly what the defining structural features would be, for a substrate that binds to hemoglobin with high affinity...
June 18, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29923455/nature-of-motor-impairments-in-autism-spectrum-disorder-a-comparison-with-developmental-coordination-disorder
#2
Aude Paquet, Bertrand Olliac, Bernard Golse, Laurence Vaivre-Douret
INTRODUCTION: Several authors have suggested the existence of motor disorders associated with developmental coordination disorder (DCD) in individuals with autism spectrum disorder (ASD). However, there are few comparative studies of psychomotor profiles that include assessments of neurological soft signs in children with ASD or DCD. We used a neuropsychomotor assessment for children with ASD from a standardized neurodevelopmental examination to understand the nature of the difficulties these children encounter...
June 20, 2018: Journal of Clinical and Experimental Neuropsychology
https://www.readbyqxmd.com/read/29906254/broad-spectrum-antimicrobial-activity-by-burkholderia-cenocepacia-tatl-371-a-strain-isolated-from-the-tomato-rhizosphere
#3
Fernando Uriel Rojas-Rojas, Anuar Salazar-Gómez, María Elena Vargas-Díaz, María Soledad Vásquez-Murrieta, Ann M Hirsch, René De Mot, Maarten G K Ghequire, J Antonio Ibarra, Paulina Estrada-de Los Santos
The Burkholderia cepacia complex (Bcc) comprises a group of 24 species, many of which are opportunistic pathogens of immunocompromised patients and also are widely distributed in agricultural soils. Several Bcc strains synthesize strain-specific antagonistic compounds. In this study, the broad killing activity of B. cenocepacia TAtl-371, a Bcc strain isolated from the tomato rhizosphere, was characterized. This strain exhibits a remarkable antagonism against bacteria, yeast and fungi including other Bcc strains, multidrug-resistant human pathogens and plant pathogens...
June 15, 2018: Microbiology
https://www.readbyqxmd.com/read/29893109/births-in-norwegian-midwife-led-birth-units-2008-10-a-population-based-study
#4
Pål Øian, Olaug Margrete Askeland, Inger Elise Engelund, Brit Roland, Marta Ebbing
BAKGRUNN: Fødestuene utgjør en del av en differensiert og desentralisert fødselsomsorg i Norge. Hensikten med studien var å undersøke forekomst og karakteristika ved planlagte og ikke-planlagte fødestuefødsler og årsaker til overflytting samt resultater for mor og barn. MATERIALE OG METODE: I perioden 2008-10 ble et tilleggsskjema til rutinemeldingen til Medisinsk fødselsregister fortløpende utfylt av jordmor for 2 514 av i alt 2 556 (98,4 %) fødestuefødsler og for 220 fødsler som var planlagt i fødestue, men der fødselen foregikk andre steder...
June 12, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
https://www.readbyqxmd.com/read/29886458/mitochondrial-peptides-modulate-mitochondrial-function-during-cellular-senescence
#5
Su-Jeong Kim, Hemal H Mehta, Junxiang Wan, Chisaka Kuehnemann, Jingcheng Chen, Ji-Fan Hu, Andrew R Hoffman, Pinchas Cohen
Cellular senescence is a complex cell fate response that is thought to underlie several age-related pathologies. Despite a loss of proliferative potential, senescent cells are metabolically active and produce energy-consuming effectors, including senescence-associated secretory phenotypes (SASPs). Mitochondria play crucial roles in energy production and cellular signaling, but the key features of mitochondrial physiology and particularly of mitochondria-derived peptides (MDPs), remain underexplored in senescence responses...
June 10, 2018: Aging
https://www.readbyqxmd.com/read/29861341/nitric-oxide-in-frozen-thawed-equine-sperm-effects-on-motility-membrane-integrity-and-sperm-capacitation
#6
André F C de Andrade, Rubens P Arruda, Mariana A Torres, Naira C G Pieri, Ticiano G Leite, Eneiva Carla C Celeghini, Leticia Z Oliveira, Thayna P Gardés, Maria Clara C Bussiere, Daniela F Silva
Nitric oxide (NO) is a reactive nitrogen species (RSN) that, over the years, has been shown to be integrated with biological and physiological events, including reproductive processes. NO can affect the functionality of spermatozoa through free radical scavenging, deactivating and inhibiting the production of superoxide anions (O2 .- ). However, the role of NO in mammalian spermatozoa physiology seems paradoxical. The aim of this study was to investigate the effects of NO on motility, hyperactivation, membrane integrity, peroxidation, and capacitation in cryopreserved equine sperm...
May 22, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29808661/hospitalisations-during-the-final-three-years-of-life
#7
Jon Ivar Elstad, Ola Reiertsen
BAKGRUNN: Kunnskap om sykehusbruk i livets sluttfase er nyttig for å forstå behovet for sykehustjenester. MATERIALE OG METODE: Registeropplysninger fra Statistisk sentralbyrå og Norsk pasientregister er brukt for å analysere tallet på innleggelser ved somatiske sykehus de siste tre leveårene blant individer som døde i alderen 56-95 år. RESULTATER: Analyseutvalget besto av 35 954 individer som hadde 136 484 innleggelser i observasjonsperioden...
May 29, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
https://www.readbyqxmd.com/read/29806306/-decompression-by-mini-open-posterior-approach-assisted-with-microscope-for-thoracolumbar-burst-fracture-with-severe-spinal-canal-stenosis
#8
Bin Zhang, Hua Zou, Weijun Kong, Chong Wang, Fujun Wu, Sheng Ye, Xin Wang, Jun Ao
Objective: To investigate the effectiveness of posterior microscopic mini-open technique (MOT) decompression in patients with severe spinal canal stenosis resulting from thoracolumbar burst fractures. Methods: The clinical data of 28 patients with severe spinal canal stenosis caused by thoracolumbar burst fractures, who were treated by posterior microscopic MOT, which performed unilateral or bilateral laminectomy, poking reduction, intervertebral bone graft via spinal canal, and percutaneous pedicle screw fixation between January 2014 and January 2016 were retrospectively analyzed...
April 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29759910/genetic-parameters-predictions-and-rankings-for-semen-production-traits-in-a-thailand-multi-breed-dairy-population-using-genomic-polygenic-and-polygenic-models
#9
Mattaneeya Sarakul, Mauricio A Elzo, Skorn Koonawootrittriron, Thanathip Suwanasopee, Danai Jattawa
The objectives were to compare estimates of variance components, genetic parameters, prediction accuracies, and rankings of bulls for semen volume (VOL), number of sperm (NS), and motility (MOT) using genomic-polygenic (GPRM) and polygenic repeatability models (PRM). The dataset comprised 13,535 VOL, 12,773 NS, and 12,660 MOT from 131 bulls collected from 2001 to 2017 in the Semen Production and Dairy Genetic Evaluation Center of the Dairy Farming Promotion Organization of Thailand. Genotypic data encompassed 76,519 actual and imputed SNP from 72 animals...
May 12, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29755402/default-mode-network-oscillatory-coupling-is-increased-following-concussion
#10
Benjamin T Dunkley, Karolina Urban, Leodante Da Costa, Simeon M Wong, Elizabeth W Pang, Margot J Taylor
Concussion is a common form of mild traumatic brain injury. Despite the descriptor "mild," a single injury can leave long-lasting and sustained alterations to brain function, including changes to localized activity and large-scale interregional communication. Cognitive complaints are thought to arise from such functional deficits. We investigated the impact of injury on neurophysiological and functionally specialized resting networks, known as intrinsic connectivity networks (ICNs), using magnetoencephalography...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29725297/1-deoxynojirimycin-dnj-ameliorates-indomethacin-induced-gastric-ulcer-in-mice-by-affecting-nf-kappab-signaling-pathway
#11
Xuehua Piao, Shuangdi Li, Xiaodan Sui, Lianyi Guo, Xingmei Liu, Hongmei Li, Leming Gao, Shusheng Cai, Yanrong Li, Tingting Wang, Baohai Liu
Gastric ulcer (GU) is a main threat to public health. 1-Deoxynojirimycin (DNJ) has antioxidant and anti-inflammatory properties and may prevent GU but related mechanism remains unclear. DNJ was extracted from the supernatants of Bacillus subtilis by using ethanol and purified by using CM-Sepharose chromatography. A GU mouse model was induced by indomethacin. The functional role of DNJ in GU mice was explored by measuring the main molecules in the NF-KappaB pathway. After the model establishment, 40 GU mice were evenly assigned into five categories: IG (received vehicle control), LG (10 μg DNJ daily), MG (20 μg DNJ daily), HG (40 μg DNJ daily), and RG (0...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29724324/effect-of-counting-chamber-depth-on-the-accuracy-of-lensless-microscopy-for-the-assessment-of-boar-sperm-motility
#12
Carles Soler, José Á Picazo-Bueno, Vicente Micó, Anthony Valverde, Daznia Bompart, Francisco J Blasco, Juan G Álvarez, Almudena García-Molina
Sperm motility is one of the most significant parameters in the prediction of male fertility. Until now, both motility analysis using an optical microscope and computer-aided sperm analysis (CASA-Mot) entailed the use of counting chambers with a depth to 20µm. Chamber depth significantly affects the intrinsic sperm movement, leading to an artificial motility pattern. For the first time, laser microscopy offers the possibility of avoiding this interference with sperm movement. The aims of the present study were to determine the different motility patterns observed in chambers with depths of 10, 20 and 100µm using a new holographic approach and to compare the results obtained in the 20-µm chamber with those of the laser and optical CASA-Mot systems...
May 4, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29713849/subjective-and-objective-assessment-of-fish-sperm-motility-when-the-technique-and-technicians-matter
#13
V Gallego, J G Herranz-Jusdado, C Rozenfeld, L Pérez, Juan F Asturiano
Fish sperm motility is nowadays considered the best sperm quality biomarker in fish, and can be evaluated both by subjective and computerized methods. With the aim to compare the precision and accuracy of both techniques, fish sperm samples were assessed by subjective methods and by a computer-assisted sperm analysis (CASA-Mot) system, and simultaneously by three different technicians with different degrees of expertise on the sperm quality analysis. Statistical dispersion parameters (CV, coefficient of variation; and RG, range) were estimated in order to determine the precision and accuracy of the techniques and the influence of laboratory staff on sperm motion assessments...
April 30, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29707864/training-with-a-three-dimensional-multiple-object-tracking-3d-mot-paradigm-improves-attention-in-students-with-a-neurodevelopmental-condition-a-randomized-controlled-trial
#14
Domenico Tullo, Jacalyn Guy, Jocelyn Faubert, Armando Bertone
The efficacy of attention training paradigms is influenced by many factors, including the specificity of targeted cognitive processes, accuracy of outcome measures, accessibility to specialized populations, and adaptability to user capability. These issues are increasingly significant when working with children diagnosed with neurodevelopmental conditions that are characterized by attentional difficulties. This study investigated the efficacy of training attention in students with neurodevelopmental conditions using a novel three-dimensional Multiple Object-Tracking (3D-MOT) task...
April 30, 2018: Developmental Science
https://www.readbyqxmd.com/read/29691953/circulating-mots-c-levels-are-decreased-in-obese-male-children-and-adolescents-and-associated-with-insulin-resistance
#15
Caiqi Du, Cai Zhang, Wei Wu, Yan Liang, Anru Wang, Shimin Wu, Yue Zhao, Ling Hou, Qin Ning, Xiaoping Luo
BACKGROUND AND AIMS: A novel bioactive peptide, mitochondrial-derived peptide (MOTS-c), has recently attracted attention as a potential prevention or therapeutic option for obesity and type 2 diabetes mellitus (T2DM). MOTS-c profiles have not yet been reported in human obesity and T2DM. We aimed to determine circulating MOTS-c levels in obesity and explore the association between MOTS-c levels and various metabolic parameters. METHODS: In this case-control study, 40 obese children and adolescents (27 males) and 57 controls (40 males) were recruited in the Hubei Province of China in 2017...
April 25, 2018: Pediatric Diabetes
https://www.readbyqxmd.com/read/29678467/effect-of-achillea-millefolium-loaded-nanophytosome-in-the-post-thawing-sperm-quality-and-oxidative-status-of-rooster-semen
#16
Davood Najafi, Ramezan Ali Taheri, Abouzar Najafi, Abbas Abbas Rouhollahi, Manuel Alvarez-Rodriguez
The aim of this study was to compare the effectiveness of antioxidants including Achillea millefolium extract (AmE) (n0t1.5: 1.5, n0t3: 3 and n0t4.5: 4.5 mg/L) and AmE loaded in nano phytosome (n1t1.5: 1.5, n1t3: 3 and n1t4.5: 4.5 mg/L) in the freezing of Ross 308 rooster semen. Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality and enzymatic parameters (SOD, CAT and GPx) were assessed after thawing. AmE-loaded nano phytosome at a concentration of 3 mg/l resulted in significantly (P < 0...
June 2018: Cryobiology
https://www.readbyqxmd.com/read/29674605/brain-training-games-enhance-cognitive-function-in-healthy-subjects
#17
Abdulrahman Al-Thaqib, Fahad Al-Sultan, Abdullah Al-Zahrani, Fahad Al-Kahtani, Khalid Al-Regaiey, Muhammad Iqbal, Shahid Bashir
BACKGROUND Brain training games (BTG) are believed to play a major role in improving cognitive functions. The current study evaluated if BTG showed positive impact on attention and memory functions compared with baseline visit in healthy subjects. MATERIAL AND METHODS The study was carried out from October 2015 until April 2016 in the Department of Physiology, College of Medicine, King Saud University and in King Khalid University Hospital (KKUH), Riyadh, Saudi Arabia. We enrolled 51 normal healthy subjects to use a computerized cognitive training game (Lumosity) for exercises that target a range of cognitive functions, including attention, processing speed, visual memory, and executive functions for about 15 min per day, at least 7 days per week, for 3 weeks...
April 20, 2018: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/29666513/bioactive-compounds-and-in-vitro-antioxidant-activity-of-some-traditional-and-non-traditional-cold-pressed-edible-oils-from-macedonia
#18
Sanja Kostadinović Veličkovska, Augustin Catalin Moţ, Saša Mitrev, Rubin Gulaboski, Ludger Brühl, Hamed Mirhosseini, Radu Silaghi-Dumitrescu, Bertrand Matthäus
The bioactive compounds and "in vitro" antioxidant activity measured by three antioxidant assays of some traditional and non-traditional cold-pressed edible oils from Macedonia were object of this study. The fatty acid composition showed dominance of monounsaturated oleic acid in "sweet" and "bitter" apricot kernel oils with percentages of 66.7 ± 0.5 and 57.8 ± 0.3%, respectively. The most dominant fatty acid in paprika seed oil was polyunsaturated linoleic acid with abundance of 69...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29663763/catheter-based-left-atrial-appendage-closure
#19
Ketil Lunde, Ahmed Al-Ani, Reidar Bjørnerheim, Anders Hervold, Anders Opdahl, Jan Otto Beitnes
BAKGRUNN: Atrieflimmer er en vanlig tilstand i befolkningen og gir økt risiko for hjerneslag. Antikoagulasjonsbehandling er effektivt for å forebygge tromboembolisme ved atrieflimmer, men av ulike grunner blir mange atrieflimmerpasienter med indikasjon for antikoagulasjonsbehandling ikke behandlet. Kateterbasert lukking av venstre atriums aurikkel er en ny metode for å forebygge hjerneslag ved atrieflimmer. MATERIALE OG METODE: I perioden september 2014-april 2016 gjennomgikk 27 pasienter med atrieflimmer og høy risiko for hjerneslag forsøk på kateterbasert lukking av venstre atriums aurikkel ved Oslo universitetssykehus...
April 17, 2018: Tidsskrift for Den Norske Lægeforening: Tidsskrift for Praktisk Medicin, Ny Række
https://www.readbyqxmd.com/read/29628020/casa-in-invertebrates
#20
Gerhard van der Horst, Monique Bennett, John D D Bishop
Sperm movement has been described in several phyla of invertebrates. Yet, sperm motility has only been quantified using computer-aided sperm analysis (CASA-Mot) in externally fertilising species (broadcast spawners) of two phyla, molluscs and echinoderms. In the present study we quantified in detail the nature of the sperm tracks, percentage motility groupings and detailed kinematics of rapid-, medium- and slow-swimming spermatozoa in the oyster Crassostrea gigas and four species never previously studied by CASA-Mot, namely the molluscs Choromytilus meridionalis, Donax serra and Haliotis midae and the echinoderm Parechinus angulosus...
April 9, 2018: Reproduction, Fertility, and Development
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