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https://www.readbyqxmd.com/read/28428259/rabl2-interacts-with-the-ift-b-complex-and-cep19-and-participates-in-ciliary-assembly
#1
Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, Kazuhisa Nakayama
Proteins localized to the basal body and the centrosome play crucial roles in ciliary assembly and functions. Although RABL2 and CEP19 are conserved in ciliated organisms and have been implicated in ciliary/flagellar functions, their roles were poorly understood. We here showed that RABL2 interacts with CEP19 and is recruited to the mother centriole and basal body in a CEP19-dependent manner, and that CEP19 is recruited to the centriole probably via its binding to the centrosomal protein FGFR1OP. Disruption of the RABL2 gene in Chlamydomonas reinhardtii resulted in the non-flagellated phenotype, suggesting a crucial role of RABL2 in ciliary/flagellar assembly...
April 20, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28423433/-myositis-ossificans-traumatica-in-the-craniocervical-junction-a-case-report-and-review-of-literature
#2
Andreas Reinke, Michael Kraus, Alexander Wild
Background Myosits ossificans (MO) is a rare but important differential diagnosis for a heterotrophic bony tumor in the muscles. It is often misdiagnosed as a malignant tumor. With a previous trauma the diagnosis is myositis ossificans traumatic (MOT). In most cases, it is benign and predominantly seen in the big muscles. But there can be malignant etiologies too. Case Description We report a rare case of MO in the muscle of the craniocervical junction. This 37-year-old woman had a riding accident years ago...
April 19, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28420194/multiple-objects-fusion-tracker-using-a-matching-network-for-adaptively-represented-instance-pairs
#3
Sang-Il Oh, Hang-Bong Kang
Multiple-object tracking is affected by various sources of distortion, such as occlusion, illumination variations and motion changes. Overcoming these distortions by tracking on RGB frames, such as shifting, has limitations because of material distortions caused by RGB frames. To overcome these distortions, we propose a multiple-object fusion tracker (MOFT), which uses a combination of 3D point clouds and corresponding RGB frames. The MOFT uses a matching function initialized on large-scale external sequences to determine which candidates in the current frame match with the target object in the previous frame...
April 18, 2017: Sensors
https://www.readbyqxmd.com/read/28417906/random-finite-set-based-bayesian-filtering-with-opencl-in-a-heterogeneous-platform
#4
Biao Hu, Uzair Sharif, Rajat Koner, Guang Chen, Kai Huang, Feihu Zhang, Walter Stechele, Alois Knoll
While most filtering approaches based on random finite sets have focused on improving performance, in this paper, we argue that computation times are very important in order to enable real-time applications such as pedestrian detection. Towards this goal, this paper investigates the use of OpenCL to accelerate the computation of random finite set-based Bayesian filtering in a heterogeneous system. In detail, we developed an efficient and fully-functional pedestrian-tracking system implementation, which can run under real-time constraints, meanwhile offering decent tracking accuracy...
April 12, 2017: Sensors
https://www.readbyqxmd.com/read/28395703/the-measurement-of-transitions-in-cancer-scale
#5
Dena Schulman-Green, Sangchoon Jeon, Ruth McCorkle, Jane Dixon
BACKGROUND AND PURPOSE: Health-illness transitions are changes in life phase, situation, or status related to shifts between health and illness. We report on the development and psychometric evaluation of the Measurement of Transitions in Cancer Scale (MOT-CA), a 7-item instrument that assesses extent and management of a range of transitions experienced by cancer patients. METHODS: We identified content domains, developed operational definitions, generated items, conducted expert review (n = 7) and cognitive interviews (n = 13), and tested MOT-CA with our target population (n = 105)...
April 1, 2017: Journal of Nursing Measurement
https://www.readbyqxmd.com/read/28387587/multiple-object-tracking-as-a-tool-for-parametrically-modulating-memory-reactivation
#6
Jordan Poppenk, Ken A Norman
Converging evidence supports the "nonmonotonic plasticity" hypothesis that, although complete retrieval may strengthen memories, partial retrieval weakens them. Yet, the classic experimental paradigms used to study effects of partial retrieval are not ideally suited to doing so, because they lack the parametric control needed to ensure that the memory is activated to the appropriate degree (i.e., that there is some retrieval but not enough to cause memory strengthening). Here, we present a novel procedure designed to accommodate this need...
April 7, 2017: Journal of Cognitive Neuroscience
https://www.readbyqxmd.com/read/28382027/potential-for-biocontrol-of-hairy-root-disease-by-a-paenibacillus-clade
#7
Lien Bosmans, Irene De Bruijn, Saskia Gerards, Rob Moerkens, Lore Van Looveren, Lieve Wittemans, Bart Van Calenberge, Anneleen Paeleman, Stefan Van Kerckhove, René De Mot, Jef Rozenski, Hans Rediers, Jos M Raaijmakers, Bart Lievens
Rhizogenic Agrobacterium biovar 1 is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato and cucumber causing significant losses in marketable yield. In order to prevent and control the disease chemical disinfectants such as hydrogen peroxide or hypochlorite are generally applied to sanitize the hydroponic system and/or hydroponic solution. However, effective control of HRD sometimes requires high disinfectant doses that may have phytotoxic effects. Moreover, several of these chemicals may be converted to unwanted by-products with human health hazards...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28378843/load-and-polysaccharide-dependent-activation-of-the-na-type-motps-stator-in-the-bacillus-subtilis-flagellar-motor
#8
Naoya Terahara, Yukina Noguchi, Shuichi Nakamura, Nobunori Kami-Ike, Masahiro Ito, Keiichi Namba, Tohru Minamino
The flagellar motor of Bacillus subtilis possesses two distinct H(+)-type MotAB and Na(+)-type MotPS stators. In contrast to the MotAB motor, the MotPS motor functions efficiently at elevated viscosity in the presence of 200 mM NaCl. Here, we analyzed the torque-speed relationship of the Bacillus MotAB and MotPS motors over a wide range of external loads. The stall torque of the MotAB and MotPS motors at high load was about 2,200 pN nm and 220 pN nm, respectively. The number of active stators in the MotAB and MotPS motors was estimated to be about ten and one, respectively...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28377707/cholinergic-potentiation-improves-perceptual-cognitive-training-of-healthy-young-adults-in-three-dimensional-multiple-object-tracking
#9
Mira Chamoun, Frédéric Huppé-Gourgues, Isabelle Legault, Pedro Rosa-Neto, Daniela Dumbrava, Jocelyn Faubert, Elvire Vaucher
A large body of literature supports cognitive enhancement as an effect of cholinergic potentiation. However, it remains elusive whether pharmacological manipulations of cholinergic neurotransmission enhance complex visual processing in healthy individuals. To test this hypothesis, we randomly administered either the cholinergic transmission enhancer donepezil (DPZ; 5 mg P.O.) or placebo (lactose) to young adults (n = 17) 3 h before each session of the three-dimensional (3D) multiple object tracking (3D-MOT) task...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28368640/collisional-cooling-of-light-ions-by-cotrapped-heavy-atoms
#10
Sourav Dutta, Rahul Sawant, S A Rangwala
We experimentally demonstrate cooling of trapped ions by collisions with cotrapped, higher-mass neutral atoms. It is shown that the lighter ^{39}K^{+} ions, created by ionizing ^{39}K atoms in a magneto-optical trap (MOT), when trapped in an ion trap and subsequently allowed to cool by collisions with ultracold, heavier ^{85}Rb atoms in a MOT, exhibit a longer trap lifetime than without the localized ^{85}Rb MOT atoms. A similar cooling of trapped ^{85}Rb^{+} ions by ultracold ^{133}Cs atoms in a MOT is also demonstrated in a different experimental configuration to validate this mechanism of ion cooling by localized and centered ultracold neutral atoms...
March 17, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28315920/-unknown-title
#11
J S Schwartz, S Al-Mot, M F Endam, S Al-Romaih, J Madrenas, M Desrosiers
BACKGROUND: Staphylococcus aureus is a frequently implicated pathogen in chronic rhinosinusitis (CRS). S. aureus may promote commensalism by downregulating pro-inflammatory T cell host responses via an IL-10 mediated pathway. This finding, coupled with the observation that S. aureus and CD8+ T cell numbers are inversely correlated in CRS mucosa, suggests that S. aureus may evade immune destruction via IL-10 induction. To support this hypothesis, we evaluated i) whether IL-10 levels differ in CRS compared to controls (CTL) using microarray and immunohistochemistry and ii) whether IL-10 levels correlate with S...
March 19, 2017: Rhinology
https://www.readbyqxmd.com/read/28304366/conditional-random-field-crf-boosting-constructing-a-robust-online-hybrid-boosting-multiple-object-tracker-facilitated-by-crf-learning
#12
Ehwa Yang, Jeonghwan Gwak, Moongu Jeon
Due to the reasonably acceptable performance of state-of-the-art object detectors, tracking-by-detection is a standard strategy for visual multi-object tracking (MOT). In particular, online MOT is more demanding due to its diverse applications in time-critical situations. A main issue of realizing online MOT is how to associate noisy object detection results on a new frame with previously being tracked objects. In this work, we propose a multi-object tracker method called CRF-boosting which utilizes a hybrid data association method based on online hybrid boosting facilitated by a conditional random field (CRF) for establishing online MOT...
March 17, 2017: Sensors
https://www.readbyqxmd.com/read/28277608/real-time-detection-of-proteolysis1-prt1-mediated-ubiquitination-via-fluorescently-labeled-substrate-probes
#13
Augustin C Mot, Erik Prell, Maria Klecker, Christin Naumann, Frederik Faden, Bernhard Westermann, Nico Dissmeyer
The N-end rule pathway has emerged as a major system for regulating protein functions by controlling their turnover in medical, animal and plant sciences as well as agriculture. Although novel functions and enzymes of the pathway have been discovered, the ubiquitination mechanism and substrate specificity of N-end rule pathway E3 ubiquitin ligases have remained elusive. Taking the first discovered bona fide plant N-end rule E3 ligase PROTEOLYSIS1 (PRT1) as a model, we used a novel tool to molecularly characterize polyubiquitination live, in real time...
March 9, 2017: New Phytologist
https://www.readbyqxmd.com/read/28276844/an-acute-interprofessional-simulation-experience-for-occupational-and-physical-therapy-students-key-findings-from-a-survey-study
#14
Erin M Thomas, Melinda F Rybski, Tonya L Apke, Deb A Kegelmeyer, Anne D Kloos
Due to the fast pace and high complexity of managing patients in intensive and acute care units (ICUs), healthcare students often feel challenged and unprepared to practice in this environment. Simulations and standardised patients provide "hands-on" learning experiences that are realistic and help students to gain competence and confidence. This study examined the impact of an intensive case simulation laboratory using a patient simulator and standardised patients on students' perceptions of their confidence and preparedness to work in acute care settings...
May 2017: Journal of Interprofessional Care
https://www.readbyqxmd.com/read/28274830/alternative-fluorimetric-based-method-to-detect-and-compare-total-s-nitrosothiols-in-plants
#15
Paulo Tamaso Mioto, Marta Rodríguez-Ruiz, Augustin Catalin Mot, Rafael Zuccarelli, Francisco J Corpas, Luciano Freschi, Helenice Mercier
Nitric oxide (NO) is an important signaling molecule occurring in virtually all organisms, whose mechanism of action relies mainly on its interaction with proteins or peptides by nitrosylation, forming compounds such as S-nitrosothiols (SNO). The Saville reaction and the ozone-based chemiluminescence method are the main techniques used for nitrosylated protein quantification. While the Saville assay is not very sensitive, the ozone-based chemiluminescence is expensive and time-consuming. Here we propose a method in which the protein-bound NO groups are exposed to UV light, cleaving the bond and allowing the quantification of the derived NO molecules by diamino-rhodamine (DAR) dyes...
March 6, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28273796/image-based-multi-target-tracking-through-multi-bernoulli-filtering-with-interactive-likelihoods
#16
Anthony Hoak, Henry Medeiros, Richard J Povinelli
We develop an interactive likelihood (ILH) for sequential Monte Carlo (SMC) methods for image-based multiple target tracking applications. The purpose of the ILH is to improve tracking accuracy by reducing the need for data association. In addition, we integrate a recently developed deep neural network for pedestrian detection along with the ILH with a multi-Bernoulli filter. We evaluate the performance of the multi-Bernoulli filter with the ILH and the pedestrian detector in a number of publicly available datasets (2003 PETS INMOVE, Australian Rules Football League (AFL) and TUD-Stadtmitte) using standard, well-known multi-target tracking metrics (optimal sub-pattern assignment (OSPA) and classification of events, activities and relationships for multi-object trackers (CLEAR MOT))...
March 3, 2017: Sensors
https://www.readbyqxmd.com/read/28242309/does-motilin-peptide-regulate-gastrointestinal-motility-of-zebrafish-an-in-vitro-study-using-isolated-intestinal-strips
#17
Takio Kitazawa, Maria Yoshida, Hiroki Teraoka, Hiroyuki Kaiya
Motilin (MOT), a 22-amino-acid peptide hormone produced in the duodenal mucosa, stimulates gastrointestinal motility in mammals and birds, and it is a mediator of interdigestive motor complexes. Recently, expression of MOT-like peptide (MOTLP) and its receptor mRNAs was identified in zebrafish. The aim of the present study was to determine whether the zebrafish MOTLP (zfMOTLP, HIAFFSPKEMRELREKE) affects zebrafish gastrointestinal motility, with comparison to the effect of human MOT, in which five amino acids are identical to zfMOTLP at positions 5, 9, 15, 16, and 17...
February 24, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28241928/n%C3%A2-341-diagnostic-et-prise-en-charge-de-la-torsion-annexielle-chez-les-filles-les-adolescentes-et-les-femmes-adultes
#18
Sari Kives, Suzy Gascon, Élise Dubuc, Nancy Van Eyk
OBJECTIF: Passer en revue les connaissances scientifiques actuelles et formuler des recommandations relatives au diagnostic et à la prise en charge de la torsion annexielle chez les filles, les adolescentes et les femmes adultes. ISSUES: L'étude porte sur les facteurs de risque, la précision diagnostique, les options de prise en charge et les issues de la torsion annexielle. RéSULTATS: Nous avons examiné les études publiées en faisant des recherches dans MEDLINE, Embase, CINAHL et la Bibliothèque Cochrane à l'aide d'une terminologie contrôlée et de mots-clés appropriés (« adnexal torsion », « ovarian torsion »)...
February 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28241534/dual-hg-rb-magneto-optical-trap
#19
Marcin Witkowski, Bartłomiej Nagórny, Rodolfo Munoz-Rodriguez, Roman Ciuryło, Piotr Szymon Żuchowski, Sławomir Bilicki, Marcin Piotrowski, Piotr Morzyński, Michał Zawada
We present a two-species laser cooling apparatus capable of simultaneously collecting Rb and Hg atomic gases into a magneto-optical trap (MOT). The atomic sources, laser system, and vacuum set-up are described. While there is a loss of Rb atoms in the MOT due to photoionization by the Hg cooling laser, we show that it does not prevent simultaneous trapping of Rb and Hg. We also demonstrate interspecies collision-induced losses in the <sup>87</sup>Rb-<sup>202</sup>Hg system.
February 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28223456/a-natural-chimeric-pseudomonas-bacteriocin-with-novel-pore-forming-activity-parasitizes-the-ferrichrome-transporter
#20
Maarten G K Ghequire, Lieselore Kemland, Ernesto Anoz-Carbonell, Susan K Buchanan, René De Mot
Modular bacteriocins represent a major group of secreted protein toxins with a narrow spectrum of activity, involved in interference competition between Gram-negative bacteria. These antibacterial proteins include a domain for binding to the target cell and a toxin module at the carboxy terminus. Self-inhibition of producers is provided by coexpression of linked immunity genes that transiently inhibit the toxin's activity through formation of bacteriocin-immunity complexes or by insertion in the inner membrane, depending on the type of toxin module...
February 21, 2017: MBio
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