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#1
J S Schwartz, S Al-Mot, M F Endam, S Al-Romaih, J Madrenas, M Desrosiers
BACKGROUND: Staphylococcus aureus is a frequently implicated pathogen in chronic rhinosinusitis (CRS). S. aureus may promote commensalism by downregulating pro-inflammatory T cell host responses via an IL-10 mediated pathway. This finding, coupled with the observation that S. aureus and CD8+ T cell numbers are inversely correlated in CRS mucosa, suggests that S. aureus may evade immune destruction via IL-10 induction. To support this hypothesis, we evaluated i) whether IL-10 levels differ in CRS compared to controls (CTL) using microarray and immunohistochemistry and ii) whether IL-10 levels correlate with S...
March 19, 2017: Rhinology
https://www.readbyqxmd.com/read/28304366/conditional-random-field-crf-boosting-constructing-a-robust-online-hybrid-boosting-multiple-object-tracker-facilitated-by-crf-learning
#2
Ehwa Yang, Jeonghwan Gwak, Moongu Jeon
Due to the reasonably acceptable performance of state-of-the-art object detectors, tracking-by-detection is a standard strategy for visual multi-object tracking (MOT). In particular, online MOT is more demanding due to its diverse applications in time-critical situations. A main issue of realizing online MOT is how to associate noisy object detection results on a new frame with previously being tracked objects. In this work, we propose a multi-object tracker method called CRF-boosting which utilizes a hybrid data association method based on online hybrid boosting facilitated by a conditional random field (CRF) for establishing online MOT...
March 17, 2017: Sensors
https://www.readbyqxmd.com/read/28277608/real-time-detection-of-proteolysis1-prt1-mediated-ubiquitination-via-fluorescently-labeled-substrate-probes
#3
Augustin C Mot, Erik Prell, Maria Klecker, Christin Naumann, Frederik Faden, Bernhard Westermann, Nico Dissmeyer
The N-end rule pathway has emerged as a major system for regulating protein functions by controlling their turnover in medical, animal and plant sciences as well as agriculture. Although novel functions and enzymes of the pathway have been discovered, the ubiquitination mechanism and substrate specificity of N-end rule pathway E3 ubiquitin ligases have remained elusive. Taking the first discovered bona fide plant N-end rule E3 ligase PROTEOLYSIS1 (PRT1) as a model, we used a novel tool to molecularly characterize polyubiquitination live, in real time...
March 9, 2017: New Phytologist
https://www.readbyqxmd.com/read/28276844/an-acute-interprofessional-simulation-experience-for-occupational-and-physical-therapy-students-key-findings-from-a-survey-study
#4
Erin M Thomas, Melinda F Rybski, Tonya L Apke, Deb A Kegelmeyer, Anne D Kloos
Due to the fast pace and high complexity of managing patients in intensive and acute care units (ICUs), healthcare students often feel challenged and unprepared to practice in this environment. Simulations and standardised patients provide "hands-on" learning experiences that are realistic and help students to gain competence and confidence. This study examined the impact of an intensive case simulation laboratory using a patient simulator and standardised patients on students' perceptions of their confidence and preparedness to work in acute care settings...
February 28, 2017: Journal of Interprofessional Care
https://www.readbyqxmd.com/read/28274830/alternative-fluorimetric-based-method-to-detect-and-compare-total-s-nitrosothiols-in-plants
#5
Paulo Tamaso Mioto, Marta Rodríguez-Ruiz, Augustin Catalin Mot, Rafael Zuccarelli, Francisco J Corpas, Luciano Freschi, Helenice Mercier
Nitric oxide (NO) is an important signaling molecule occurring in virtually all organisms, whose mechanism of action relies mainly on its interaction with proteins or peptides by nitrosylation, forming compounds such as S-nitrosothiols (SNO). The Saville reaction and the ozone-based chemiluminescence method are the main techniques used for nitrosylated protein quantification. While the Saville assay is not very sensitive, the ozone-based chemiluminescence is expensive and time-consuming. Here we propose a method in which the protein-bound NO groups are exposed to UV light, cleaving the bond and allowing the quantification of the derived NO molecules by diamino-rhodamine (DAR) dyes...
March 6, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28273796/image-based-multi-target-tracking-through-multi-bernoulli-filtering-with-interactive-likelihoods
#6
Anthony Hoak, Henry Medeiros, Richard J Povinelli
We develop an interactive likelihood (ILH) for sequential Monte Carlo (SMC) methods for image-based multiple target tracking applications. The purpose of the ILH is to improve tracking accuracy by reducing the need for data association. In addition, we integrate a recently developed deep neural network for pedestrian detection along with the ILH with a multi-Bernoulli filter. We evaluate the performance of the multi-Bernoulli filter with the ILH and the pedestrian detector in a number of publicly available datasets (2003 PETS INMOVE, Australian Rules Football League (AFL) and TUD-Stadtmitte) using standard, well-known multi-target tracking metrics (optimal sub-pattern assignment (OSPA) and classification of events, activities and relationships for multi-object trackers (CLEAR MOT))...
March 3, 2017: Sensors
https://www.readbyqxmd.com/read/28242309/does-motilin-peptide-regulate-gastrointestinal-motility-of-zebrafish-an-in-vitro-study-using-isolated-intestinal-strips
#7
Takio Kitazawa, Maria Yoshida, Hiroki Teraoka, Hiroyuki Kaiya
Motilin (MOT), a 22-amino-acid peptide hormone produced in the duodenal mucosa, stimulates gastrointestinal motility in mammals and birds, and it is a mediator of interdigestive motor complexes. Recently, expression of MOT-like peptide (MOTLP) and its receptor mRNAs was identified in zebrafish. The aim of the present study was to determine whether the zebrafish MOTLP (zfMOTLP, HIAFFSPKEMRELREKE) affects zebrafish gastrointestinal motility, with comparison to the effect of human MOT, in which five amino acids are identical to zfMOTLP at positions 5, 9, 15, 16, and 17...
February 24, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28241928/n%C3%A2-341-diagnostic-et-prise-en-charge-de-la-torsion-annexielle-chez-les-filles-les-adolescentes-et-les-femmes-adultes
#8
Sari Kives, Suzy Gascon, Élise Dubuc, Nancy Van Eyk
OBJECTIF: Passer en revue les connaissances scientifiques actuelles et formuler des recommandations relatives au diagnostic et à la prise en charge de la torsion annexielle chez les filles, les adolescentes et les femmes adultes. ISSUES: L'étude porte sur les facteurs de risque, la précision diagnostique, les options de prise en charge et les issues de la torsion annexielle. RéSULTATS: Nous avons examiné les études publiées en faisant des recherches dans MEDLINE, Embase, CINAHL et la Bibliothèque Cochrane à l'aide d'une terminologie contrôlée et de mots-clés appropriés (« adnexal torsion », « ovarian torsion »)...
February 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28241534/dual-hg-rb-magneto-optical-trap
#9
Marcin Witkowski, Bartłomiej Nagórny, Rodolfo Munoz-Rodriguez, Roman Ciuryło, Piotr Szymon Żuchowski, Sławomir Bilicki, Marcin Piotrowski, Piotr Morzyński, Michał Zawada
We present a two-species laser cooling apparatus capable of simultaneously collecting Rb and Hg atomic gases into a magneto-optical trap (MOT). The atomic sources, laser system, and vacuum set-up are described. While there is a loss of Rb atoms in the MOT due to photoionization by the Hg cooling laser, we show that it does not prevent simultaneous trapping of Rb and Hg. We also demonstrate interspecies collision-induced losses in the <sup>87</sup>Rb-<sup>202</sup>Hg system.
February 20, 2017: Optics Express
https://www.readbyqxmd.com/read/28223456/a-natural-chimeric-pseudomonas-bacteriocin-with-novel-pore-forming-activity-parasitizes-the-ferrichrome-transporter
#10
Maarten G K Ghequire, Lieselore Kemland, Ernesto Anoz-Carbonell, Susan K Buchanan, René De Mot
Modular bacteriocins represent a major group of secreted protein toxins with a narrow spectrum of activity, involved in interference competition between Gram-negative bacteria. These antibacterial proteins include a domain for binding to the target cell and a toxin module at the carboxy terminus. Self-inhibition of producers is provided by coexpression of linked immunity genes that transiently inhibit the toxin's activity through formation of bacteriocin-immunity complexes or by insertion in the inner membrane, depending on the type of toxin module...
February 21, 2017: MBio
https://www.readbyqxmd.com/read/28194143/novel-immunity-proteins-associated-with-colicin-m-like-bacteriocins-exhibit-promiscuous-protection-in-pseudomonas
#11
Maarten G K Ghequire, Lieselore Kemland, René De Mot
Bacteriocins related to colicin M, acting via cleavage of the cell wall precursor lipid II, have been characterized in γ- and β-proteobacteria. Depending on the species, immunity is provided by either an inner membrane-anchored periplasmic protein or by an integral membrane protein. In Pseudomonas however, the immunity partner of colicin M-like bacteriocins remains unknown. Based on an in silico analysis in pseudomonad genomes, we here identify a gene encoding a putative immunity partner that represents a novel type of integral membrane protein (PmiA, Pseudomonas colicin M-like immunity type A)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28161171/technical-note-effects-of-attachment-of-hind-teats-before-cleaning-and-attachment-of-front-teats-on-milking-characteristics-in-automatic-milking-systems
#12
J Besier, G Schüpbach-Regula, O Wellnitz, R M Bruckmaier
Milking characteristics differ between the 4 quarters of a dairy cow udder. In particular, milking time is mostly prolonged in hind quarters compared with front quarters because of the usually higher amount of stored milk. The standard milking routine (STDMR) in both conventional and automatic milking systems (AMS) consists of teat preparation of all 4 quarters, followed by attachment of the 4 teat cups, regardless of the distribution of milk between quarters. In the current study, an alternative teat preparation and milking routine (ALTMR) in AMS was tested, which consisted of cleaning and starting the milking of hind teats before cleaning and attachment of front teats...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28139357/morphological-brain-changes-associated-with-negative-symptoms-in-patients-with-22q11-2-deletion-syndrome
#13
Angeline Mihailov, Maria Carmela Padula, Elisa Scariati, Marie Schaer, Maude Schneider, Stephan Eliez
Approximately 30% of individuals with 22q11.2 Deletion Syndrome (22q11DS) develop schizophrenia during adolescence/early adulthood, making this syndrome a model for the disorder. Furthermore, negative symptoms exist in up to 80% of patients diagnosed with 22q11DS. The present study aims to uncover morphological brain alterations associated with negative symptoms in a cohort of patients with 22q11DS who are at-risk for developing schizophrenia. A total of 71 patients with 22q11DS aged 12 to 35 (54% females) with no past or present diagnosis of a schizophrenia were included in the study...
January 27, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28130552/melatonin-micronutrients-osteopenia-treatment-study-mots-a-translational-study-assessing-melatonin-strontium-citrate-vitamin-d3-and-vitamin-k2-mk7-on-bone-density-bone-marker-turnover-and-health-related-quality-of-life-in-postmenopausal-osteopenic-women-following
#14
Sifat Maria, Mark H Swanson, Larry T Enderby, Frank D'Amico, Brianna Enderby, Rebekah M Samsonraj, Amel Dudakovic, Andre J van Wijnen, Paula A Witt-Enderby
This one-year double blind randomized control trial assessed the effects of nightly melatonin, strontium (citrate), vitamin D3 and vitamin K2 (MK7; MSDK) on bone mineral density (BMD) and quality of life (QOL) in postmenopausal osteopenic women (ages 49-75). Compared to placebo, MSDK treatment increased BMD in lumbar spine (4.3%) and left femoral neck (2.2%), with an upward trend for total left hip (p=0.069). MSDK increased serum P1NP levels and reduced bone turnover (CTx:P1NP). Psychometric analyses indicated that mood and sleep quality improved for the MSDK group...
January 26, 2017: Aging
https://www.readbyqxmd.com/read/28127511/high-angular-resolution-diffusion-imaging-tractography-of-cerebellar-pathways-from-newborns-to-young-adults
#15
Thomas J Re, Jacob Levman, Ashley R Lim, Andrea Righini, Patricia Ellen Grant, Emi Takahashi
INTRODUCTION: Many neurologic and psychiatric disorders are thought to be due to, or result in, developmental errors in neuronal cerebellar connectivity. In this connectivity analysis, we studied the developmental time-course of cerebellar peduncle pathways in pediatric and young adult subjects. METHODS: A cohort of 80 subjects, newborns to young adults, was studied on a 3T MR system with 30 diffusion-weighted measurements with high-angular resolution diffusion imaging (HARDI) tractography...
January 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28114965/synergistic-antibacterial-effect-of-co-administering-adipose-derived-mesenchymal-stromal-cells-and-ophiophagus-hannah-l-amino-acid-oxidase-in-a-mouse-model-of-methicillin-resistant-staphylococcus-aureus-infected-wounds
#16
Yee Yik Mot, Iekhsan Othman, Syed Hassan Sharifah
BACKGROUND: Mesenchymal stromal cells (MSCs) and Ophiophagus hannah L-amino acid oxidase (Oh-LAAO) have been reported to exhibit antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). Published data have indicated that synergistic antibacterial effects could be achieved by co-administration of two or more antimicrobial agents. However, this hypothesis has not been proven in a cell- and protein-based combination. In this study, we investigate if co-administration of adipose-derived MSCs and Oh-LAAO into a mouse model of MRSA-infected wounds would be able to result in a synergistic antibacterial effect...
January 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28112718/erratum-indexing-the-pseudomonas-specialized-metabolome-enabled-the-discovery-of-poaeamide-b-and-the-bananamides
#17
Don D Nguyen, Alexey V Melnik, Nobuhiro Koyama, Xiaowen Lu, Michelle Schorn, Jinshu Fang, Kristen Aguinaldo, Tommie L Lincecum, Maarten G K Ghequire, Victor J Carrion, Tina L Cheng, Brendan M Duggan, Jacob G Malone, Tim H Mauchline, Laura M Sanchez, A Marm Kilpatrick, Jos M Raaijmakers, René De Mot, Bradley S Moore, Marnix H Medema, Pieter C Dorrestein
No abstract text is available yet for this article.
January 23, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28111352/the-effect-of-negative-emotion-on-multiple-object-tracking-task-an-erp-study
#18
Jing Su, Dongyuan Duan, Xuemin Zhang, Huanyu Lei, Chundi Wang, Heng Guo, Xiaoqian Yan
Previous studies have revealed that negative emotion may influence participants' cognitive processing. However, the neural mechanism of the impact of negative emotion on dynamic task like Multiple Object Tracking (MOT) is still unknown. This present study used electrophysiological (Event-Related Potentials, ERP) measures to investigate the effect of negative emotion on MOT tasks. Participants were required to complete MOT tasks while detecting the probe dots that would appear on targets, distractors or the space between them during tracking...
January 19, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28111190/increased-sensitivity-to-age-related-differences-in-brain-functional-connectivity-during-continuous-multiple-object-tracking-compared-to-resting-state
#19
Erlend S Dørum, Tobias Kaufmann, Dag Alnæs, Ole A Andreassen, Geneviève Richard, Knut K Kolskår, Jan Egil Nordvik, Lars T Westlye
Age-related differences in cognitive agility vary greatly between individuals and cognitive functions. This heterogeneity is partly mirrored in individual differences in brain network connectivity as revealed using resting-state functional magnetic resonance imaging (fMRI), suggesting potential imaging biomarkers for age-related cognitive decline. However, although convenient in its simplicity, the resting state is essentially an unconstrained paradigm with minimal experimental control. Here, based on the conception that the magnitude and characteristics of age-related differences in brain connectivity is dependent on cognitive context and effort, we tested the hypothesis that experimentally increasing cognitive load boosts the sensitivity to age and changes the discriminative network configurations...
January 20, 2017: NeuroImage
https://www.readbyqxmd.com/read/28081584/-mini-open-approach-shows-good-results-in-treatment-of-femoroacetabular-impingement
#20
Stephanie Maria Kirschbaum, Frank Sitterlee, Carsten Perka, Michael Müller
Background Femoroacetabular impingement (FAI) describes a painful, abnormal (bony) contact between the femoral neck and acetabulum, which, if left untreated, contributes to early osteoarthritis (OA) of the hip. Despite its flat learning curve and long operation times, hip arthroscopy has been used increasingly often in recent years in the surgery of femoroacetabular impingement. The mini-open technique (MOT) offers a possible alternative that addresses the abnormal morphological prominence and allows minimally invasive clearance of hip motion...
January 12, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
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