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https://www.readbyqxmd.com/read/29134651/heavy-metal-contamination-microbiological-spoilage-and-biogenic-amines-content-in-sushi-available-on-the-polish-market
#1
Piotr Kulawik, Dani Dordevic, Florian Gambuś, Katarzyna Szczurowska, Marzena Zając
BACKGROUND: The study determined the heavy metal contamination (mercury, cadmium, lead, arsenic and nickel) of nori, restaurant-served sushi and ready-to-eat sushi meals available through retail chains. Moreover the microbiological loads and biogenic amines content in ready-to-eat sushi meals were analysed. RESULTS: All the nori samples contained high levels of Cd (2.122 mg kg(-1) ), Ni (0.715 mg kg(-1) ), As (34.56 mg kg(-1) ) and Pb (0.659 mg kg(-1) ). The studied sushi samples contained high levels of Ni and Pb, reaching 0...
November 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29050898/sushi-cervice-and-gnathostomiasis-a-case-report-and-review-of-imported-infections
#2
REVIEW
Jordan Leroy, Marjorie Cornu, Anne Sophie Deleplancque, Séverine Loridant, Emmanuel Dutoit, Boualem Sendid
We describe a case of imported cutaneous gnathostomiasis in a Thai patient living in France. Gnathostomiasis is a zoonosis of food origin. The disease is endemic in Southeast Asia and Latin America. However, over the past 30 years, an increasing number of imported cases has been described in Europe and America. The disease is rare in Western Europe and the majority of cases described had a cutaneous clinical presentation. The disease may sometimes be confused with allergy, leading to a delay in diagnosis. Visceral symptoms are rare but may follow severe attacks...
October 16, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/28936667/rna-seq-data-analysis-from-raw-data-quality-control-to-differential-expression-analysis
#3
Weihong Qi, Ralph Schlapbach, Hubert Rehrauer
As a revolutionary technology for life sciences, RNA-seq has many applications and the computation pipeline has also many variations. Here, we describe a protocol to perform RNA-seq data analysis where the aim is to identify differentially expressed genes in comparisons of two conditions. The protocol follows the recently published RNA-seq data analysis best practice and applies quality checkpoints throughout the analysis to ensure reliable data interpretation. It is written to help new RNA-seq users to understand the basic steps necessary to analyze an RNA-seq dataset properly...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28936214/a-mucosal-and-cutaneous-chemokine-ligand-for-the-lymphocyte-chemoattractant-receptor-gpr15
#4
Borja Ocón, Junliang Pan, Theresa Thu Dinh, Wenjing Chen, Romain Ballet, Michael Bscheider, Aida Habtezion, Hua Tu, Brian A Zabel, Eugene C Butcher
Chemoattractants control lymphocyte recruitment from the blood, contributing to the systemic organization of the immune system. The G protein-linked receptor GPR15 mediates lymphocyte homing to the large intestines and skin. Here we show that the 9 kDa CC-motif containing cationic polypeptide AP57/colon-derived sushi containing domain-2 binding factor (CSBF), encoded by C10orf99 in the human and 2610528A11Rik in the mouse, functions as a chemokine ligand for GPR15 (GPR15L). GPR15L binds GPR15 and attracts GPR15-expressing T cells including lymphocytes in colon-draining lymph nodes and Vγ3(+) thymic precursors of dermal epithelial T cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28901431/isolated-chromosome-8p23-2%C3%A2-pter-deletion-novel-evidence-for-developmental-delay-intellectual-disability-microcephaly-and-neurobehavioral-disorders
#5
Shanshan Shi, Shaobin Lin, Baojiang Chen, Yi Zhou
The current study presents a patient carrying a de novo ~6 Mb deletion of the isolated chromosome 8p23.2‑pter that was identified with a single‑nucleotide polymorphism array. The patient was characterized by developmental delay (DD)/intellectual disability (ID), microcephaly, autism spectrum disorder, attention‑deficit/hyperactivity disorders and mildly dysmorphic features. The location, size and gene content of the deletion observed in this patient were compared with those in 7 patients with isolated 8p23...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28867754/sushi-repeat-containing-protein-x-linked-2-promotes-angiogenesis-through-the-urokinase-type-plasminogen-activator-receptor-dependent-integrin-%C3%AE-v%C3%AE-3-focal-adhesion-kinase-pathways
#6
Kuiliang Liu, Jianghao Fan, Jing Wu
Sushi repeat-containing protein X-linked 2 (SRPX2) is a newly identified chondroitin sulfate proteoglycan that is markedly elevated in multiple solid tumors. It is also suggested that SRPX2 is associated with angiogenesis. A conditioned medium of SRPX2 overexpressing colorectal cancer (CRC) cells and SRPX2 recombinant protein was used to evaluate the effect of secretory SRPX2 on the angiogenesis ability of human umbilical vein endothelial cells (HUVECs) and the involved molecular mechanisms. It was revealed that the activity of SRPX2 is dependent on the urokinase-type plasminogen activator receptor and cooperation of the integrin αvβ3 co-receptor...
2017: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/28841682/downregulation-of-endometrial-mesenchymal-marker-susd2-causes-cell-senescence-and-cell-death-in-endometrial-carcinoma-cells
#7
Shaqiu Zhang, Ni Zeng, Nour Alowayed, Yogesh Singh, Anchun Cheng, Florian Lang, Madhuri S Salker
The cause of death among the majority of endometrial cancer patients involves migration of cancer cells within the peritoneal cavity and subsequent implantation of cancer spheroids into neighbouring organs. It is, thereby, important to identify factors that mediate metastasis. Cell adhesion and migration are modified by the mesenchymal stem cell (MSC) marker Sushi domain containing 2 (SUSD2), a type I transmembrane protein that participates in the orchestration of cell adhesion and migration through interaction with its partner Galactosidase-binding soluble-1 (LGALS1)...
2017: PloS One
https://www.readbyqxmd.com/read/28724927/dual-functionality-nanobioconjugates-targeting-intracellular-bacteria-in-cancer-cells-with-enhanced-antimicrobial-activity
#8
Rohini Singh, Smita Patil, Neetu Singh, Shalini Gupta
Bacterial drug resistance has emerged as a serious global threat mandating the development of novel methodologies that allow facile modulation of antimicrobial action in a controlled fashion. Conjugating antibiotics to nanoparticles helps to meet this goal by increasing the drug's overall avidity, bioavailability and easier internalisation into mammalian cells, targeting bacteria that otherwise escape antibacterial action by host cell-localisation. We used polymyxin B sulfate (PMB) and sushi peptide as model drugs against Gram-negative bacteria and established their enhanced antimicrobial activity on Escherichia coli (E...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719914/the-significance-of-antibodies-against-domain-i-of-beta-2-glycoprotein-i-in-antiphospholipid-syndrome
#9
Hilde Kelchtermans, Walid Chayouâ, Bas de Laat
The antiphospholipid syndrome (APS) is characterized by vascular thrombosis and/or pregnancy morbidity with the persistent presence of antiphospholipid antibodies (aPLs). Progress is being made in understanding the pathogenesis of the syndrome, but difficulties persist in the identification of patients at risk for thrombosis and/or pregnancy morbidity. Beta-2 glycoprotein I (β2GPI), a plasma protein consisting of five sushi domains, is thought to be the main antigenic target of aPLs. Antibodies recognizing domain I of β2GPI are predominantly present in patients with an elevated risk of thrombosis, whereas antidomain IV/V antibodies are found in nonthrombotic autoimmune diseases...
July 18, 2017: Seminars in Thrombosis and Hemostasis
https://www.readbyqxmd.com/read/28675105/salt-microspheres-and-potassium-chloride-usage-for-sodium-reduction-case-study-with-sushi
#10
Đani Đorđević, Hana Buchtová, Blanka Macharáčková
The aim of the study was to estimate possibilities of salt substitutes usage in the preparation of two sushi types (nigiri and maki) prepared with different seafood (salmon: Salmo salar, tuna: Thunnus albacares, and shrimp: Pleoticus muelleri). Potassium chloride (Mary samples), Soda-Lo (hollowed microsphere of regular salt crystals), and regular salt (sodium chloride) were used in the experiment. Sushi samples (n = 1960) were evaluated by 40 trained panelists who noticed that maki shrimp samples prepared with Mary salt had higher bitterness (21...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28654796/srpx2-an-independent-prognostic-marker-promotes-cell-migration-and-invasion-in-hepatocellular-carcinoma
#11
Xiaobo Lin, Weiping Chang, Yuan Wang, Ming Tian, Zhaoxiang Yu
Sushi repeat-containing protein X-linked 2 (SRPX2), a novel chondroitin sulfate proteoglycan, is overexpressed in human cancer. Recent studies have reported that SRPX2 overexpression is observed in gastrointestinal cancer, and promotes migration and invasion of cancer cells. While, the clinical significance and biological function of SRPX2 remain rarely known in hepatocellular carcinoma (HCC). Here, we found that the levels of SRPX2 in HCC tissues were notably overexpressed compared to non-cancerous specimens...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28654203/effects-of-storage-temperature-on-bacterial-growth-rates-and-community-structure-in-fresh-retail-sushi
#12
S Hoel, A N Jakobsen, O Vadstein
AIMS: This study was conducted to assess the effects of different storage temperatures (4-20°C), on bacterial concentrations, growth rates and community structure in fresh retail sushi, a popular retail product with a claimed shelf life of 2-3 days. METHODS AND RESULTS: The maximum specific growth rate based on aerobic plate count (APC) at 4°C was 0·06 h(-1) and displayed a sixfold increase (0·37 h(-1) ) at 20°C. Refrigeration resulted in no growth of hydrogen sulphide (H2 S)-producing bacteria, but this group had the strongest temperature response...
June 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28619796/draft-genome-sequence-of-campylobacter-jejuni-cam970-and-c-%C3%A2-coli-cam962-associated-with-a-large-outbreak-of-foodborne-illness-in-fukuoka-japan-in-2016
#13
Hiroshi Asakura, Naoto Takahashi, Shiori Yamamoto, Hiroyuki Maruyama
Here, we report the draft genome sequences of Campylobacter jejuni CAM970 and C. coli CAM962, which were associated with a large outbreak of foodborne illness originating from undercooked chicken sushi in Fukuoka, Japan, in May 2016. Their genome sizes were 1,690,901 and 1,704,736 bp, with 22 and 23 rRNAs, 9 and 9 tRNAs, and 411× and 419× coverage for C. jejuni CAM970 and C. coli CAM962, respectively.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28599077/protective-efficacy-of-recombinant-cryptosporidium-parvum-cpprp1-sushi-domain-against-c-tyzzeri-infection-in-mice
#14
Y Huang, W Cao, K Shi, R Mi, K Lu, X Han, Z Chen
Until now, there are no completely effective parasite-specific pharmaceuticals or immunotherapies for treatment against the zoonotic cryptosporidiosis. Sushi domain (CpSushi) is an important functional domain in Cryptosporidium parvum putative rhoptry protein-1 (CpPRP1), which is the only reported C. parvum rhoptry protein and may play key role in the course of invasion. Here, a 708-bp fragment encoding the CpSushi domain was amplified and expressed in E. coli. Immunofluorescence detection showed that CpSushi was located on the surface of C...
September 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28596762/species-distribution-and-prevalence-of-putative-virulence-factors-in-mesophilic-aeromonas-spp-isolated-from-fresh-retail-sushi
#15
Sunniva Hoel, Olav Vadstein, Anita N Jakobsen
Aeromonas spp. are ubiquitous bacteria that have received increasing attention as human pathogens because of their widespread occurrence in food, especially seafood and vegetables. The aim of this work was to assess the species identity and phylogenetic relationship of 118 Aeromonas strains isolated from fresh retail sushi from three producers, and to characterize the isolates with respect to genetic and phenotypic virulence factors. We also evaluate the potential hazard associated with their presence in ready-to-eat seafood not subjected to heat treatment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28534981/loss-of-csmd1-expression-disrupts-mammary-duct-formation-while-enhancing-proliferation-migration-and-invasion
#16
Mohamed Kamal, Deborah L Holliday, Ewan E Morrison, Valerie Speirs, Carmel Toomes, Sandra M Bell
The CUB and sushi multiple domains 1 (CSMD1) gene maps to chromosome 8p23, a region deleted in many cancers. Loss of CSMD1 expression is associated with poor prognosis in breast cancer suggesting that it acts as a tumour suppressor in this cancer. However, the function of CSMD1 is largely unknown. Herein, we investigated CSMD1 functions in cell line models. CSMD1 expression was suppressed in MCF10A and LNCaP cells using short hairpin RNA. Functional assays were performed focusing on the 'normal' MCF10A cell line...
May 22, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28475599/susd2-promotes-tumor-associated-macrophage-recruitment-by-increasing-levels-of-mcp-1-in-breast-cancer
#17
Elizabeth M Hultgren, Mitch E Patrick, Rick L Evans, Catherine T Stoos, Kristi A Egland
Tumor-associated macrophages (TAMs) play a role in tumor angiogenesis and are recruited into the tumor microenvironment (TME) by secreted chemokines, including Monocyte Chemoattractant Protein-1 (MCP-1/CCL2). Angiogenesis is required to sustain proliferation and enable metastasis of breast cancer (BCa) cells. Understanding the underlying mechanisms of TAM recruitment would allow for the identification of desperately needed novel drug targets. Sushi Domain Containing 2 (SUSD2), a transmembrane protein on BCa cells, was previously shown to promote tumor angiogenesis in a murine model...
2017: PloS One
https://www.readbyqxmd.com/read/28462733/epidemiology-and-whole-genome-sequencing-of-an-ongoing-point-source-salmonella-agona-outbreak-associated-with-sushi-consumption-in-western-sydney-australia-2015
#18
C K Thompson, Q Wang, S K Bag, N Franklin, C T Shadbolt, P Howard, E J Fearnley, H E Quinn, V Sintchenko, K G Hope
During May 2015, an increase in Salmonella Agona cases was reported from western Sydney, Australia. We examine the public health actions used to investigate and control this increase. A descriptive case-series investigation was conducted. Six outbreak cases were identified; all had consumed cooked tuna sushi rolls purchased within a western Sydney shopping complex. Onset of illness for outbreak cases occurred between 7 April and 24 May 2015. Salmonella was isolated from food samples collected from the implicated premise and a prohibition order issued...
July 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28437165/survey-for-listeria-monocytogenes-in-and-on-ready-to-eat-foods-from-retail-establishments-in-the-united-states-2010-through-2013-assessing-potential-changes-of-pathogen-prevalence-and-levels-in-a-decade
#19
John B Luchansky, Yuhuan Chen, Anna C S Porto-Fett, Régis Pouillot, Bradley A Shoyer, Rachel Johnson-DeRycke, Denise R Eblen, Karin Hoelzer, William K Shaw, Jane M van Doren, Michelle Catlin, Jeehyun Lee, Rohan Tikekar, Daniel Gallagher, James A Lindsay, Sherri Dennis
A multiyear interagency Listeria monocytogenes Market Basket Survey was undertaken for selected refrigerated ready-to-eat foods purchased at retail in four FoodNet sites in the United States. Food samples from 16 food categories in six broad groups (seafood, produce, dairy, meat, eggs, and combination foods) were collected weekly at large national chain supermarkets and independent grocery stores in California, Maryland, Connecticut, and Georgia for 100 weeks between December 2010 and March 2013. Of the 27,389 total samples, 116 samples tested positive by the BAX PCR system for L...
June 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28393817/efficacy-of-triphala-extract-and-chlorhexidine-mouth-rinse-against-plaque-accumulation-and-gingival-inflammation-among-female-undergraduates-a-randomized-controlled-trial
#20
Sahana Umesh Baratakke, Rekha Raju, Sushi Kadanakuppe, Nagashree R Savanur, Radha Gubbihal, Pallavi Swamy Kousalaya
AIM: To know the efficacy of Triphala extract and Chlorhexidine mouth rinse against plaque and gingival inflammation. MATERIALS AND METHODS: A double blinded parallel arm randomised control trial was done among 60 participants aged 18-24 years. Participants were randomly allotted to three groups with 20 participants in each group of 0.6% triphala, 0.12% chlorhexidine and control group. Study was done in 2 phases of 21 days duration. During the experimental period, participants rinsed with the allocated mouth rinse 10ml twice daily for 30 seconds without any supervision...
January 2017: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
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