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María E Errasti, Claudia L Natalucci, Néstor O Caffini, Alejandra E Rotelli, Adriana Brullo, Bruno Maras, Sebastián A Trejo, Laura M I López
The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 61,685 M-1  cm-1 . The alignment of the sequence of macrodontain I with those cysteine peptidases from species belonging to the family Bromeliaceae showed the highest identity degree (87.74%) against fruit bromelain...
March 15, 2018: Applied Biochemistry and Biotechnology
Diego Vallés, Ana M B Cantera
Crude extract (CE) from pulp of Bromelia antiacantha Bertol. mature fruit, contains at least 3 cysteine proteases with proteolytic activity. By single step cation exchange chromatography (Hi-trap SP-HP) of partially purified CE, the protease with the lowest pI, Antiacanthain A (AntA), was isolated. It showed maximum activity at pH 9, and 75% of remaining activity was maintained over a wide pH range (pH 6-10). The AntA activity exhibits a constant increase up to 70 °C. Maintains almost 100% of its activity at 45 at pH 6 and 9...
March 6, 2018: International Journal of Biological Macromolecules
Sara H Mohamed, Mahmoud S M Mohamed, Mary S Khalil, Magda Azmy, Mona I Mabrouk
AIM: This study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae METHODS AND RESULTS: Effect of seven EOs and three enzymes was tested on formation and eradication of Klebsiella pneumoniae biofilm. Peppermint oil showed robust biofilm inhibitory effect, causing inhibition ranged from 69.2-98.2% at 5 μl ml-1 . Thyme oil was found to have the best biofilm-eradication ability, causing eradication ranged from 80...
March 8, 2018: Journal of Applied Microbiology
Jigna R Bhamore, Sanjay Jha, Hirakendu Basu, Rakesh Kumar Singhal, Z V P Murthy, Suresh Kumar Kailasa
Herein, fluorescent gold nanoclusters (Au NCs) were obtained by one-pot synthetic method using bovine serum albumin (BSA) and bromelain as templates. As-synthesized fluorescent Au NCs were stable and showed bright red fluorescence under UV lamp at 365 nm. The fluorescent Au NCs exhibit the emission intensity at 648 nm when excited at 498 nm. Various techniques were used such as spectroscopy (UV-visible, fluorescence, and Fourier-transform infrared), high-resolution transmission electron microscopy, and dynamic light scattering for the characterization of fluorescent Au NCs...
February 26, 2018: Analytical and Bioanalytical Chemistry
Yi Tan, Ping Li
The purpose of this study was to investigate the application of bromelain in the treatment of pain, swelling, and limited mouth opening in patients with hematologic tumor after extraction of the third molar during chemotherapy. We recruited 72 patients with hematologic tumor who were treated by oral and maxillofacial surgery in our hospital, and third molar extraction was performed under local anesthesia. After extraction, patients in the control group were treated with cold - hot compress and patients in the experimental group were treated by oral administration of bromelain...
March 2018: Oncology Letters
Thomas A Prohaska, Xuchu Que, Cody J Diehl, Sabrina Hendrikx, Max W Chang, Kristen Jepsen, Christopher K Glass, Christopher Benner, Joseph L Witztum
B-1 cells are a unique subset of B cells that are positively selected for expressing autoreactive BCRs. We isolated RNA from peritoneal (B-1a, B-1b, B-2) and splenic (B-1a, marginal zone, follicular) B cells from C57BL/6 mice and used 5'-RACE to amplify the IgH V region using massively parallel sequencing. By analyzing 379,000 functional transcripts, we demonstrate that B-1a cells use a distinct and restricted repertoire. All B-1 cell subsets, especially peritoneal B-1a cells, had a high proportion of sequences without N additions, suggesting predominantly prenatal development...
January 29, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Giulia Maria Sancesario, Marzia Nuccetelli, Andrea Cerri, Joshua Zegeer, Cinzia Severini, Maria Teresa Ciotti, Massimo Pieri, Alessandro Martorana, Carlo Caltagirone, Robert Nistico, Sergio Bernardini
BACKGROUND: Therapeutic approaches targeting amyloid β-42 (Aβ42) oligomers may represent a promising neuroprotective strategy for the prevention and treatment of Alzheimer's disease (AD). OBJECTIVE: In this study we evaluated the ability of bromelain, a plant cysteine protease derived from pineapple stems, to interact with synthetic Aβ42 monomers and oligomers. We also examined the ability of bromelain to interfere in vitro with synthetic Aβ42 aggregates in the cerebrospinal fluid (CSF) of Alzheimer's disease as well as of control patients affected by other neurological diseases...
January 23, 2018: Current Alzheimer Research
Chanikan Sonklin, Natta Laohakunjit, Orapin Kerdchoechuen, Khanok Ratanakhanokchai
Enzymatic mungbean meal protein hydrolysate (eb-MPH) is a novel natural flavour/antioxidant source. A 15% bromelain (w/w) concentration with a hydrolysis time of 12 h was the optimum conditions to produce eb-MPH, which showed the greatest antioxidant activities and sensory characteristics. eb-MPH was composed of oligopeptides that had low molecular weight (< 10 kDa) as well as surface hydrophobicity and high content of hydrophobic amino acids. eb-MPH contributed to DPPH (80) and ABTS (95%) scavenging activities and to savoury/sweet flavour volatile compounds (3-methyl-butanal, furfural and benzaldehyde), bouillon odour, sweet odour, chicken odour, meaty odour, moderate bitter taste and umami...
January 2018: Journal of Food Science and Technology
Fatahiya Mohamed Tap, Fadzilah Adibah Abd Majid, Hassan Fahmi Ismail, Tet Soon Wong, Kamyar Shameli, Mikio Miyake, Nurul Bahiyah Ahmad Khairudin
Phospholipase A2 (Pla2) is an enzyme that induces inflammation, making Pla2 activity an effective approach to reduce inflammation. Therefore, investigating natural compounds for this Pla2 inhibitory activity has important therapeutic potential. The objective of this study was to investigate the potential in bromelain-phytochemical complex inhibitors via a combination of in silico and in vitro methods. Bromelain-amenthoflavone displays antagonistic effects on Pla2. Bromelian-asiaticoside and bromelain-diosgenin displayed synergistic effects at high concentrations of the combined compounds, with inhibition percentages of more than 70% and 90%, respectively, and antagonistic effects at low concentrations...
January 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Kavya Bhakuni, Pannuru Venkatesu
Proteins in vivo are under an extremely crowded environment because of the presence of bulky and large biological macromolecules (known as crowders). These crowders affect the proper functioning and structure of proteins in a cell. During in vitro studies, we often ignore the effect of macromolecular crowding on protein stability. However, if a large concentration of crowder is used to examine protein stability, its effects on the functioning of protein inside the cell in a confined environment, as stated, can be understood...
December 14, 2017: International Journal of Biological Macromolecules
Shehu Muhammad Auwal, Mohammad Zarei, Azizah Abdul-Hamid, Nazamid Saari
Protein hydrolysates produced from different food sources exhibit therapeutic potential and can be used in the management of chronic diseases. This study was targeted to optimise the conditions for the hydrolysis of stone fish protein to produce antioxidant hydrolysates using central composite design (CCD) by response surface methodology (RSM). The stone fish protein was hydrolysed under the optimum predicted conditions defined by pH (6.5), temperature (54°C), E/S ratio (1.5%), and hydrolysis time (360 min)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Shehu Muhammad Auwal, Mohammad Zarei, Chin Ping Tan, Mahiran Basri, Nazamid Saari
Recent biotechnological advances in the food industry have led to the enzymatic production of angiotensin I-converting enzyme (ACE)-inhibitory biopeptides with a strong blood pressure lowering effect from different food proteins. However, the safe oral administration of biopeptides is impeded by their enzymatic degradation due to gastrointestinal digestion. Consequently, nanoparticle (NP)-based delivery systems are used to overcome these gastrointestinal barriers to maintain the improved bioavailability and efficacy of the encapsulated biopeptides...
December 2, 2017: Nanomaterials
Zijuan Zhou, Liang Wang, Panpan Feng, Lianhong Yin, Chen Wang, Shengxu Zhi, Jianyi Dong, Jingyu Wang, Yuan Lin, Dapeng Chen, Yongjian Xiong, Jinyong Peng
Activation of the TNF-α receptor (TNFR) leads to an inflammatory response, and anti-TNF therapy has been administered to reduce inflammation symptoms and heal mucosal ulcers in inflammatory bowel disease (IBD). Bromelain, a complex natural mixture of proteolytic enzymes, has been shown to exert anti-inflammatory effects. This study aimed to investigate the effect of purified fruit bromelain (PFB)-induced inhibition of epithelial TNFR in a rat colitis model. Colitis was established by intracolonic administration of 2, 4, 6-trinitrobenzene sulfonic acid...
2017: Frontiers in Immunology
Hsin-Chieh Lin, Adeola M Alashi, Rotimi E Aluko, Bonnie Sun Pan, Yu-Wei Chang
Proteins from tilapia frame and skin can potentially be precursors of antihypertensive peptides according to the result of BIOPEP analyses. The aim was to generate peptides with inhibitory effects against angiotensin-converting enzyme (ACE) and renin from tilapia frame and skin protein isolates (FPI and SPI). The most active hydrolysate was then tested for blood pressure-lowering ability in spontaneously hypertensive rats (SHRs). Tilapia frame and skin protein hydrolysates (FPHs and SPHs) were respectively produced from FPI and SPI hydrolysis using pepsin, papain, or bromelain...
2017: Food & Nutrition Research
Kholoud Awad Al-Shamsi, Priti Mudgil, Hassan Mohamed Hassan, Sajid Maqsood
Camel milk protein hydrolysates (CMPH) were generated using proteolytic enzymes, such as alcalase, bromelain, and papain, to explore the effect on the technofunctional properties and antioxidant potential under in vitro and in real food model systems. Characterization of the CMPH via degree of hydrolysis, sodium dodecyl sulfate-PAGE, and HPLC revealed that different proteins in camel milk underwent degradation at different degrees after enzymatic hydrolysis using 3 different enzymes for 2, 4, and 6 h, with papain displaying the highest degradation...
November 8, 2017: Journal of Dairy Science
Khalid El-Bahrawy, Sherif Rateb, Marwa Khalifa, Davide Monaco, Giovanni Lacalandra
This investigation aimed to determine the influence of using different techniques for liquefaction of semen on post-thaw physical and dynamic characteristics of camel spermatozoa. A total of 144 ejaculates were collected from 3 adult camels, Camelus dromedarius, twice-weekly over 3 consecutive breeding seasons. A raw aliquot of each ejaculate was evaluated for physical and morphological properties, whereas the remaining portion was diluted (1:3) with glycerolated Tris lactose egg yolk extender, and was further subjected to one of the following liquefaction treatments: control (untreated), 5μl/ml α-amylase, 0...
October 31, 2017: Animal Reproduction Science
Desiderio Passali, Luisa Maria Bellussi, Codrut Sarafoleanu, Michele Loglisci, Claudiu Manea, Cristina Iosif, Francesco Maria Passali
No abstract text is available yet for this article.
July 2017: Journal of Pharmacology & Pharmacotherapeutics
Nuri Ari Efiana, Thi Nhu Quynh Phan, Arko Jatmiko Wicaksono, Andreas Bernkop-Schnürch
This study aimed to improve the mucus permeating properties of self-emulsifying drug delivery systems (SEDDS) by anchoring lipidized bromelain, papain and trypsin using palmitoyl chloride. SEDDS containing enzyme-palmitate conjugates were characterized regarding droplet size and zeta potential. Their mucus permeating properties were evaluated by Transwell diffusion and rotating tube method using fluorescein diacetate (FDA) as marker. Degree of substitution of modified enzymes was 35.3%, 47.8% and 38.5% for bromelain-palmitate, papain-palmitate and trypsin-palmitate, respectively...
October 23, 2017: Colloids and Surfaces. B, Biointerfaces
Claudia Menzel, Andreas Bernkop-Schnürch
The use of mucus permeating drug carrier systems being able to overcome the mucus barrier can lead to a remarkable enhancement in bioavailability. One promising approach is the design of mucolytic enzyme decorated carrier systems (MECS). These systems include micro- and nanoparticles as well as self-emulsifying drug delivery systems (SEDDS) decorated with mucin cleaving enzymes such as papain (PAP) or bromelain (BRO). MECS are able to cross the mucus barrier in a comparatively efficient manner by cleaving mucus substructures in front of them on their way to the epithelium...
October 24, 2017: Advanced Drug Delivery Reviews
Cristina Botinestean, Carolina Gomez, Yingqun Nian, Mark A E Auty, Joseph P Kerry, Ruth M Hamill
Meat intakes in the older population are commonly reduced because the relatively tough texture of meat can impair mastication. Fruit-derived proteolytic enzymes have been reported to have beneficial effects on tenderness, by causing significant degradation of myofibrillar proteins and collagen. Three treatments including: papain, bromelain and a 50:50 mixture of papain/bromelain, alongside one control were applied to beef M. semitendinosus steaks. Effects on Warner - Bratzler shear force, texture parameters, color, and cook loss were determined...
October 25, 2017: Journal of Texture Studies
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