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Chiara Di Tucci, Mara Di Feliciantonio, Flaminia Vena, Carmela Capone, Michele Carlo Schiavi, Daniela Pietrangeli, Ludovico Muzii, Pierluigi Benedetti Panici
Alpha-Lipoic acid (ALA) is a natural antioxidant synthetized by plants and animals, identified as a catalytic agent for oxidative decarboxylation of pyruvate and α-ketoglutarate. In this review, we analyzed the action of ALA in gynecology and obstetrics focusing in particular on neuropathic pain and antioxidant and anti-inflammatory action. A comprehensive literature search was performed in PubMed and Cochrane Library for retrieving articles in English language on the antioxidant and anti-inflammatory effects of ALA in gynecological and obstetrical conditions...
May 4, 2018: Gynecological Endocrinology
Sung Sil Moon
The effects of proteolytic enzymes (bromelain and bromelain+papain) and a ginger extract were assessed on collagen content and solubility, thermal shrinkage temperature of connective tissue, pH, cooking loss, drip loss, and Warner-Bratzler shear force (WBSF) of M. pectoralis profundus isolated from the beef brisket cut. Both proteolytic enzymes and ginger extract led to a significant increase in cooking loss and collagen solubility compared with untreated controls. On the other hand, the peak ( T p ) thermal shrinkage temperature markedly decreased in all treatments compared with those in controls...
February 2018: Korean Journal for Food Science of Animal Resources
Rik Osinga, Peter Steiger, Pietro Giovanoli, Jan A Plock, Lijo Mannil
INTRODUCTION: Thermal injuries are frequent and can be associated with relevant morbidity and mortality in severe cases. Excisional debridement followed by autografting is the standard of care for deep burns, but is associated with serious potential complications. Clinical results of a novel enzymatic debridement based on Bromelain raw material extracted from the pineapple plant (Nexobrid™) were published in 2014. At the Center for Burns at the University Hospital Zürich enzymatic debridement was introduced in 2016...
April 26, 2018: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
Yaron Shoham, Yuval Krieger, Eran Tamir, Eldad Silberstein, Alexander Bogdanov-Berezovsky, Josef Haik, Lior Rosenberg
Sharp debridement is currently considered most effective for debridement of chronic wounds; however, some patients do not have access to or cannot be treated by surgical methods. This study was designed to provide a first impression of the safety and efficacy of bromelain-based enzymatic debridement of chronic wounds. Two consecutive single-arm studies assessing the enzymatic debridement efficacy of a concentrate of proteolytic enzymes enriched in bromelain in chronic wounds was conducted in 2 medical centres...
April 25, 2018: International Wound Journal
Xin Wang, Le He, Bing Wei, Guoqing Yan, Jun Wang, Rupei Tang
No abstract text is available yet for this article.
April 14, 2018: International Journal of Biological Macromolecules
Adam J Singer, Jimmy Toussaint, Won Taek Chung, Steve A McClain, Richard A F Clark, Eilon Asculai, Dafna Geblinger, Lior Rosenberg
OBJECTIVES: There are no well accepted animal models of chronic wounds, limiting advances in understanding and treatment of chronic ulcers. We developed a porcine wound model which combines multiple factors involved in chronic wounds to create a contaminated necrotic eschar and evaluated the debriding efficacy of a novel bromelain based enzymatic debriding agent (EscharEx). METHODS: Contaminated ischemic wounds were created on the flanks of domestic pigs by 'sandwiching' the skin between 2 'O' rings (1 placed on the surface of the skin and the other underneath the skin) for 24h prior to dermatomal excision of the necrotic eschar and its contamination with Staphylococcus aureus and Candida albicans...
April 13, 2018: Burns: Journal of the International Society for Burn Injuries
Janaína Artem Ataide, Eloah Favero Gérios, Priscila Gava Mazzola, Eliana B Souto
Stem bromelain is a common available cysteine protease derived from pineapple (Ananas comosus L.). Bromelain finds widespread applications in several areas, such as medicine, health, food, and cosmetics, and its strong proteolytic activity supports its future application in many additional fields. However, most proteins and/or enzymes are fragile, leading to important considerations about increase storage and operational stability to enable their practical application. In this scenario, the use of nanoparticles to deliver proteins is increasing exponentially, given that these systems are capable of enhance active's stability, solubility and permeability, and decrease toxicity...
March 27, 2018: Advances in Colloid and Interface Science
Yu Fu, Jing Liu, Erik T Hansen, Wender L P Bredie, René Lametsch
The aim of this study was to use different enzyme mixtures to investigate the influence of peptide characteristics and taste of protein hydrolysates from bovine muscle and porcine plasma. Minced beef and porcine plasma were hydrolysed using 10 food-grade enzymes, including Protease A, Protease P, ProteAX, Flavourzyme, Alcalase, Papain, Bromelain, Protamex, Neutrase and Sumizyme BNP-L. The relationship between degree of hydrolysis (DH), molecular weight (MW) distribution, enzyme specificity, and sensory characteristics of hydrolysates were investigated...
August 15, 2018: Food Chemistry
Xinya Xu, Ruilin Liu, Pengqi Guo, Zhimin Luo, Xiang Cai, Hua Shu, Yanhui Ge, Chun Chang, Qiang Fu
Bromelain, a cysteine endopeptidase enzyme of great commercial value, has been widely used in food, pharmaceutical, and cosmetic industries. Conventional methods for purification of bromelain are still limited by a low binding efficiency, time-consuming process, and expensive equipment. Therefore, for selective absorption of bromelain, we developed a facile and effective method to fabricate magnetic mesoporous molecularly imprinted polymers using pericarpium granati-derived carbon as the carrier for the first time...
August 1, 2018: Food Chemistry
Feridun Gürlek, Mehmet Ünsel, Ömür Ardeniz, Zeynep Peker Koc, Okan Gülbahar, Aytül Zerrin Sin, Ali Kokuludag, Nihal Mete Gokmen
BACKGROUND: It has been suggested that latex-specific IgE analysis may lead to false-positive results, especially in patients with pollen allergy. In the present study, the reasons underlying clinically irrelevant latex-specific IgE positivity were investigated. METHODS: Thirty patients with latex allergy (group 1), 89 patients sensitised to aeroallergens (group 2a), and 98 healthy individuals without allergy (group 2b) were enrolled. Participants from all 3 groups were subjected to skin prick tests with aeroallergens including latex, latex-specific IgE analysis (ImmunoCAP), and nasal provocation test with latex...
2018: International Archives of Allergy and Immunology
Mohamad Ariff Hanafi, Siti Nadia Hashim, Shyan Yea Chay, Afshin Ebrahimpour, Mohammad Zarei, Kharidah Muhammad, Azizah Abdul-Hamid, Nazamid Saari
As a protein-rich, underutilized crop, green soybean could be exploited to produce hydrolysates containing angiotensin-I converting enzyme (ACE) inhibitory peptides. Defatted green soybean was hydrolyzed using four different food-grade proteases (Alcalase, Papain, Flavourzyme and Bromelain) and their ACE inhibitory activities were evaluated. The Alcalase-generated green soybean hydrolysate showed the highest ACE inhibitory activity (IC50 : 0.14 mg/mL at 6 h hydrolysis time) followed by Papain (IC50 : 0...
April 2018: Food Research International
Yew L Loo, Benjamin K L Goh, S Jeffery
Aim: Recent introduction of rapid bromelain-based enzymatic debridement has been increasingly popular in its use in non-surgical debridement in deep partial and full thickness burns. We designed this study to evaluate the evidence suggested by current studies on the perceived benefits of using Nexobrid® as compared to traditional surgical standard of care (SOC) in burns wound debridement. Method: A comprehensive search on electronic databases Pubmed, Embase and Web of Science was done to identify studies published between 1986 to 2017 involving the use of Nexobrid in deep partial and full thickness burns...
March 22, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
Aaron S Griffin, Peter Cabot, Ben Wallwork, Ben Panizza
The use of alternative medicine in chronic rhinosinusitis (CRS) continues to increase in popularity, for the most part without meeting the burden of being based on sound clinical evidence. New and emerging treatments, both natural and developed, are numerous, and it remains a challenge for otolaryngologists as well as general practitioners to keep up to date with these therapies and their efficacy. In this systematic review, we discuss a number of alternative therapies for CRS, their proposed physiologic mechanisms, and evidence supporting their use...
March 2018: Ear, Nose, & Throat Journal
María E Errasti, Claudia L Natalucci, Néstor O Caffini, Alejandra E Rotelli, Adriana Brullo, Bruno Maras, Sebastián A Trejo, Laura M I López
The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 61,685 M-1  cm-1 . The alignment of the sequence of macrodontain I with those cysteine peptidases from species belonging to the family Bromeliaceae showed the highest identity degree (87.74%) against fruit bromelain...
March 15, 2018: Applied Biochemistry and Biotechnology
Diego Vallés, Ana M B Cantera
Crude extract (CE) from pulp of Bromelia antiacantha Bertol. mature fruit, contains at least 3 cysteine proteases with proteolytic activity. By single step cation exchange chromatography (Hi-trap SP-HP) of partially purified CE, the protease with the lowest pI, Antiacanthain A (AntA), was isolated. It showed maximum activity at pH9, and 75% of remaining activity was maintained over a wide pH range (pH6-10). The AntA activity exhibits a constant increase up to 70°C. Maintains almost 100% of its activity at 45 at pH6 and 9...
July 1, 2018: International Journal of Biological Macromolecules
S H Mohamed, M S M Mohamed, M S Khalil, M Azmy, M I Mabrouk
AIM: This study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae. METHODS AND RESULTS: The effect of seven EOs and three enzymes was tested on formation and eradication of K. pneumoniae biofilm. Peppermint oil showed a robust biofilm inhibitory effect, causing inhibition that ranged from 69·2 to 98·2% at 5 μl ml-1 . Thyme oil was found to have the best biofilm eradication ability, causing eradication that ranged from 80·1 to 98·0% at 10 μl ml-1 ...
March 8, 2018: Journal of Applied Microbiology
Jigna R Bhamore, Sanjay Jha, Hirakendu Basu, Rakesh Kumar Singhal, Z V P Murthy, Suresh Kumar Kailasa
Herein, fluorescent gold nanoclusters (Au NCs) were obtained by one-pot synthetic method using bovine serum albumin (BSA) and bromelain as templates. As-synthesized fluorescent Au NCs were stable and showed bright red fluorescence under UV lamp at 365 nm. The fluorescent Au NCs exhibit the emission intensity at 648 nm when excited at 498 nm. Various techniques were used such as spectroscopy (UV-visible, fluorescence, and Fourier-transform infrared), high-resolution transmission electron microscopy, and dynamic light scattering for the characterization of fluorescent Au NCs...
April 2018: Analytical and Bioanalytical Chemistry
Yi Tan, Ping Li
The purpose of this study was to investigate the application of bromelain in the treatment of pain, swelling, and limited mouth opening in patients with hematologic tumor after extraction of the third molar during chemotherapy. We recruited 72 patients with hematologic tumor who were treated by oral and maxillofacial surgery in our hospital, and third molar extraction was performed under local anesthesia. After extraction, patients in the control group were treated with cold - hot compress and patients in the experimental group were treated by oral administration of bromelain...
March 2018: Oncology Letters
Thomas A Prohaska, Xuchu Que, Cody J Diehl, Sabrina Hendrikx, Max W Chang, Kristen Jepsen, Christopher K Glass, Christopher Benner, Joseph L Witztum
B-1 cells are a unique subset of B cells that are positively selected for expressing autoreactive BCRs. We isolated RNA from peritoneal (B-1a, B-1b, B-2) and splenic (B-1a, marginal zone, follicular) B cells from C57BL/6 mice and used 5'-RACE to amplify the IgH V region using massively parallel sequencing. By analyzing 379,000 functional transcripts, we demonstrate that B-1a cells use a distinct and restricted repertoire. All B-1 cell subsets, especially peritoneal B-1a cells, had a high proportion of sequences without N additions, suggesting predominantly prenatal development...
March 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Giulia Maria Sancesario, Marzia Nuccetelli, Andrea Cerri, Joshua Zegeer, Cinzia Severini, Maria Teresa Ciotti, Massimo Pieri, Alessandro Martorana, Carlo Caltagirone, Robert Nistico, Sergio Bernardini
BACKGROUND: Therapeutic approaches targeting amyloid β-42 (Aβ42) oligomers may represent a promising neuroprotective strategy for the prevention and treatment of Alzheimer's disease (AD). OBJECTIVE: In this study we evaluated the ability of bromelain, a plant cysteine protease derived from pineapple stems, to interact with synthetic Aβ42 monomers and oligomers. We also examined the ability of bromelain to interfere in vitro with synthetic Aβ42 aggregates in the cerebrospinal fluid (CSF) of Alzheimer's disease as well as of control patients affected by other neurological diseases...
January 23, 2018: Current Alzheimer Research
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