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https://www.readbyqxmd.com/read/28644207/enzymatic-versus-traditional-surgical-debridement-of-severely-burned-hands-a-comparison-of-selectivity-efficacy-healing-time-and-three-month-scar-quality
#1
Alexandra Schulz, Yaron Shoham, Lior Rosenberg, Irene Rothermund, Walther Perbix, Paul Christian Fuchs, Alexandra Lipensky, Jennifer Lynn Schiefer
Severe burns of the hands are extremely challenging, given their anatomic complexity and vulnerability. Although excisional debridement with autografting remains the standard of care (SOC), previous studies have shown that use of enzymatic debridement with bromelain (NexoBrid, EDNX) enables rapid, selective enzymatic debridement, preserving viable tissue. To date, only two studies accruing data on EDNX in this setting have been published. The current study was conducted to compare EDNX with traditional surgical debridement (TSD) of deep dermal and full-thickness hand burns...
July 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28629290/functional-foods-how-functional-are-they-a-case-report-of-supplement-induced-psychosis
#2
Priyanka Yadav, Kyle Stigall, Hannah E Johnson, Abner O Rayapati, Nitin Chopra
There is rising evidence of patients' use of alternative and complementary medicine. The percentage of the U.S. population who used at least one dietary supplement increased from 42% in 1988-1994 to 53% in 2003-2006. We present a case of an Asian female in her 40s, with no previous psychiatric illness, who presented to the emergency room following a brief psychotic episode, during which she self-amputated the tips of her fingers, after using multivitamins and herbal supplements including ginseng, gui yuan rou (Chinese herb), astaxanthin, goji (Chinese fruit), selenium, saw palmetto, grape seed extract, citrus bioflavanoid, lutein (zeaxantin), resvexatrol, sun chlorella, spirulina powder, phytoceramides, phytoestrogen, glucosatrin, bromelain plus, and American bee pollen...
August 2016: International Journal of Psychiatry in Medicine
https://www.readbyqxmd.com/read/28618976/bromelain-decorated-hybrid-nanoparticles-based-on-lactobionic-acid-conjugated-chitosan-for-in%C3%A2-vitro-anti-tumor-study
#3
Bing Wei, Le He, Xin Wang, Guo Qing Yan, Jun Wang, Rupei Tang
In this work, lactobionic acid-modified chitosan (CLA) was chosen as an initial material to prepare tumor-targeted nanoparticles (CLA NPs). To improve the nanoparticles' tumor penetration ability, bromelain was then decorated on the surface of CLA NPs to give CLAB NPs. The micromorphology of CLA and CLAB NPs was observed by transmission electron microscopy and scanning electron microscopy. The stability of CLA and CLAB NPs was then investigated at different pH values (4.0-9.0) and physiological environment by dynamic light scattering...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28616992/coherent-experimental-and-simulation-approach-to-explore-the-underlying-mechanism-of-denaturation-of-stem-bromelain-in-osmolytes
#4
Anjeeta Rani, Mohamed Taha, Pannuru Venkatesu, Ming-Jer Lee
Characterization of a protein in the context of its environment is of crucial importance for a complete understanding of its function. Although biophysical techniques provide powerful tools for studying the stability and activity of the enzyme in the presence of various cosolvents, an approach of combining both experimental techniques and molecular dynamic (MD) simulations may lead to the mechanistic insight into the interactions governing the stability of an enzyme. The knowledge of these interactions can be further utilized for range of modifications in the wild form of an enzyme for various pharmaceutical applications...
June 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28580904/fabrication-of-core-shell-nanofibers-for-controlled-delivery-of-bromelain-and-salvianolic-acid-b-for-skin-regeneration-in-wound-therapeutics
#5
Ekambaram Shoba, Rachita Lakra, Manikantan Syamala Kiran, Purna Sai Korrapati
The physiological and pathological complexity of the wound healing process makes it more challenging to design an ideal tissue regeneration scaffold. Precise scaffolding with high drug loading efficiency, efficient intracellular efficacy for therapeutic delivery, minimal nonspecific cellular and blood protein binding, and maximum biocompatibility forms the basis for an ideal delivery system. This paper describes a combinational multiphasic delivery system, where biomolecules are delivered through the fabrication of coaxial electrospinning of different biocompatible polymers...
June 5, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28548677/destabilization-of-human-insulin-fibrils-by-peptides-of-fruit-bromelain-derived-from-ananas-comosus-pineapple
#6
Sromona Das, Debasish Bhattacharyya
Deposition of insulin aggregates in human body leads to dysfunctioning of several organs. Effectiveness of fruit bromelain from pineapple in prevention of insulin aggregate was investigated. Proteolyses of bromelain was done as par human digestive system and the pool of small peptides was separated from larger peptides and proteins. Under conditions of growth of insulin aggregates from its monomers, this pool of peptides restricted the reaction upto formation of oligomers of limited size. These peptides also destabilized preformed insulin aggregates to oligomers...
May 26, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28512541/anti-plaque-and-anti-gingivitis-effect-of-papain-bromelain-miswak-and-neem-containing-dentifrice-a-randomized-controlled-trial
#7
Abhinav Tadikonda, Kalyana-Chakravarthy Pentapati, Arun-Sreenivas Urala, Shashidhar Acharya
BACKGROUND: Patients undergoing fixed orthodontic therapy may have difficulty in maintaining a good oral hygiene due to the difficulty posed by the appliances in accessing such areas. This study aimed to compare anti-plaque and anti-gingivitis efficacy of dentifrice containing Papain, Bromelain, Miswak and Neem with a standard dentifrice among patient's undergoing fixed orthodontic treatment. MATERIAL AND METHODS: Single center, single blind, parallel arm, randomized controlled clinical trial with an allocation ratio of 1:1 was conducted...
May 2017: Journal of Clinical and Experimental Dentistry
https://www.readbyqxmd.com/read/28506851/immunocap-cellulose-displays-cross-reactive-carbohydrate-ccd-epitopes-and-can-cause-false-positive-test-results-in-patients-with-high-anti-ccd-ige-antibody-levels
#8
Wolfgang Hemmer, Friedrich Altmann, Friedrich Holzweber, Clemens Gruber, Felix Wantke, Stefan Wöhrl
RATIONALE: Cross-reactive carbohydrate determinants (CCDs) in plants and insect venoms are a common cause of irrelevant positive test results during in-vitro allergy diagnosis. We observed that some CCD-positive sera show non-specific IgE-binding even with CCD-free recombinant allergens when using the Phadia ImmunoCAP platform. OBJECTIVE: We investigated if cellulose used as allergen carrier in the ImmunoCAP harbors residual N-glycans causing non-specific background-binding in CCD-positive sera...
May 12, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28497911/the-clinical-efficacy-of-pollen-extract-and-vitamins-on-chronic-prostatitis-chronic-pelvic-pain-syndrome-is-linked-to-a-decrease-in-the-pro-inflammatory-cytokine-interleukin-8
#9
Tommaso Cai, Paolo Verze, Roberto La Rocca, Alessandro Palmieri, Daniele Tiscione, Lorenzo Giuseppe Luciani, Sandra Mazzoli, Vincenzo Mirone, Gianni Malossini
PURPOSE: We aim to evaluate the efficacy of pollen extract in association with vitamins in patients affected by chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and to evaluate the level of the pro-inflammatory mediators interleukin (IL)-6, IL-8, and IL-10. MATERIALS AND METHODS: Patients diagnosed with CP/CPPS between January and December 2015 were enrolled in this study. Participants were randomly assigned to receive oral capsules of pollen extract and vitamins (group A) or bromelain (group B) for 3 months...
April 30, 2017: World Journal of Men's Health
https://www.readbyqxmd.com/read/28493022/in-vitro-anti-thrombotic-and-anti-coagulant-properties-of-blacklip-abalone-haliotis-rubra-viscera-hydrolysate
#10
Hafiz Ansar Rasul Suleria, Paul P Masci, Rama Addepalli, Wei Chen, Glenda C Gobe, Simone A Osborne
Abalone viscera contain sulphated polysaccharides with anti-thrombotic and anti-coagulant activities. In this study, a hydrolysate was prepared from blacklip abalone (Haliotis rubra) viscera using papain and bromelain and fractionated using ion exchange and size exclusion chromatography. Hydrolysates and fractions were investigated for in vitro thrombin inhibition mediated through heparin cofactor II (HCII) as well as anti-coagulant activity in plasma and whole blood. On the basis of sulphated polysaccharide concentration, the hydrolysate inhibited thrombin through HCII with an inhibitor concentration at 50% (IC50) of 16...
May 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28441786/application-of-pineapple-juice-in-the-fish-digestion-process-for-carcinogenic-liver-fluke-metacercaria-collection
#11
Panupan Sripan, Ratchadawan Aukkanimart, Thidarut Boonmars, Pranee Sriraj, Jiraporn Songsri, Parichart Boueroy, Sukhonthip Khueangchaingkhwang, Benjamabhorn Pumhirunroj, Atchara Artchayasawat
Pepsin is common digestive enzyme used for fish digestion in the laboratory to collect trematode metacercariae. In a field study, to survey the infected fish is needed a huge yield of pepsin and it is very expensive. Therefore, our purpose of this study was to investigate the candidate enzyme from pineapple juice which has a digestive enzyme called bromelain, a mixture of proteolytic enzymes, to digest fish in order to harvest metacercariae. Fish were divided into 2 groups: one group in which metacercariae were harvested using acid pepsin as a control and other groups in which the fish was digested using fresh pineapple juices...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28439939/identification-of-a-polygalacturonase-cup-s-2-as-the-major-ccd-bearing-allergen-in-cupressus-sempervirens-pollen
#12
Y Shahali, J-P Sutra, C Hilger, K Swiontek, I Haddad, J Vinh, L Guilloux, D Charpin, H Sénéchal, P Poncet
As IgE glyco-epitopes, also referred to as cross-reactive carbohydrate determinants (CCDs), can share significant structural homologies between different plants, they are prone to extensive cross-reactivity among allergen pollen extracts. Here, cypress pollen allergens, especially a polygalacturonase (PG), were further characterized using double one-dimensional electrophoresis (D1-DE). The presence of specific IgE directed against CCDs was investigated by bromelain IgE inhibition and concanavalin A binding assays using sera of cypress pollen-sensitized patients...
April 25, 2017: Allergy
https://www.readbyqxmd.com/read/28418600/validation-of-a-laboratory-developed-test-of-human-sperm-capacitation
#13
Melissa A Moody, Cristina Cardona, Alana J Simpson, T Timothy Smith, Alexander J Travis, G Charles Ostermeier
Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not respond to stimuli for capacitation. Sperm with "capacitated" patterns had exposed acrosomal carbohydrates and underwent acrosome exocytosis in response to calcium ionophore (A23187). Precision was evaluated by percent change of the Cap-Score measured for 50, 100, 150, and 200 sperm...
May 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28394630/plant-proteases-for-bioactive-peptides-release-a-review
#14
M A Mazorra-Manzano, J C Ramírez-Suarez, R Y Yada
Proteins are a potential source of health promoting biomolecules with medical, nutraceutical and food applications. Nowadays, bioactive peptides production, its isolation, characterization and strategies for its delivery to target sites are a matter of intensive research. In vitro and in vivo studies regarding the bioactivity of peptides has generated strong evidence of their health benefits. Dairy proteins are considered the richest source of bioactive peptides, however proteins from animal and vegetable origin also have been shown to be important sources...
April 10, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28392381/the-light-subunit-of-mushroom-agaricus-bisporus-tyrosinase-its-biological-characteristics-and-implications
#15
Wangsa T Ismaya, Olivia M Tandrasasmita, Syaripah Sundari, Diana, Xuelei Lai, Debbie S Retnoningrum, Bauke W Dijkstra, Raymond R Tjandrawinata, Heni Rachmawati
The light subunit of mushroom Agaricus bisporus tyrosinase (LSMT) is a protein of unknown function that was discovered serendipitously during the elucidation of the crystal structure of the enzyme. The protein is non-immunogenic and can penetrate the intestinal epithelial cell barrier, and thus, similar to its structural homologue HA-33 from Clostridium botulinum, may be potentially absorbable by the intestine. LSMT also shares high structural homology with the ricin-B-like lectin from the mushroom Clitocybe nebularis (CNL), which has been shown to display biological activity against leukemic cancer cells and dendritic cells...
April 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28363663/enzymatic-debridement-of-deeply-burned-faces-healing-and-early-scarring-based-on-tissue-preservation-compared-to-traditional-surgical-debridement
#16
Alexandra Schulz, Paul Christian Fuchs, Irene Rothermundt, Alexandra Hoffmann, Lior Rosenberg, Yaron Shoham, Henrik Oberländer, Jennifer Schiefer
INTRODUCTION: Facial burns occur frequently and depending on the injured skin layers often heal with scars which may cause permanent functional and cosmetic sequelae. Preservation of the sensitive facial skin layers, especially of the dermis is essential for scarless epithelialisation. Enzymatic debridement of deep thermal burns has already been shown to assist with preserving viable dermis. However, up to date, there are no published reports on wound healing and in the long term aesthetic outcome after enzymatic debridement of facial burns...
March 28, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28362352/optimization-of-bromelain-aided-production-of-angiotensin-i-converting-enzyme-inhibitory-hydrolysates-from-stone-fish-using-response-surface-methodology
#17
Shehu Muhammad Auwal, Mohammad Zarei, Azizah Abdul-Hamid, Nazamid Saari
The stone fish is an under-utilized sea cucumber with many nutritional and ethno-medicinal values. This study aimed to establish the conditions for its optimum hydrolysis with bromelain to generate angiotensin I-converting enzyme (ACE)-inhibitory hydrolysates. Response surface methodology (RSM) based on a central composite design was used to model and optimize the degree of hydrolysis (DH) and ACE-inhibitory activity. Process conditions including pH (4-7), temperature (40-70 °C), enzyme/substrate (E/S) ratio (0...
March 31, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28352546/bioactivities-of-fish-protein-hydrolysates-from-defatted-salmon-backbones
#18
Rasa Slizyte, Katariina Rommi, Revilija Mozuraityte, Peter Eck, Kathrine Five, Turid Rustad
Bioactivities of bulk fish protein hydrolysates (FPH) from defatted salmon backbones obtained with eight different commercial enzymes and their combinations were tested. All FPH showed antioxidative activity in vitro. DPPH scavenging activity increased, while iron chelating ability decreased with increasing time of hydrolysis. All FPH showed ACE inhibiting effect which depended on type of enzyme and increased with time of hydrolysis. The highest effect was found for FPH produced with Trypsin. Bromelain + Papain hydrolysates reduced the uptake of radiolabelled glucose into CaCo-2 cells, a model of human enterocytes, indicating a potential antidiabetic effect of FPH...
September 2016: Biotechnology Reports
https://www.readbyqxmd.com/read/28341257/our-initial-learning-curve-in-the-enzymatic-debridement-of-severely-burned-hands-management-and-pit-falls-of-initial-treatments-and-our-development-of-a-post-debridement-wound-treatment-algorithm
#19
A Schulz, W Perbix, Y Shoham, S Daali, C Charalampaki, P C Fuchs, J Schiefer
INTRODUCTION: Excisional surgical debridement (SD) is still the gold standard in the treatment of deeply burned hands, though the intricate anatomy is easily damaged. Previous studies demonstrated that enzymatic debridement with the bromelain debriding agent NexoBrid(®) (EDNX) is more selective and thus can preserve viable tissue with excellent outcome results. So far no method paper has been published presenting different treatment algorithms in this new field. Therefore our aim was to close this gap by presenting our detailed learning curve in EDNX of deeply burned hands...
March 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28321764/protective-manifestation-of-bacoside-a-and-bromelain-in-terms-of-cholinesterases-gamma-amino-butyric-acid-serotonin-level-and-stress-proteins-in-the-brain-of-dichlorvos-intoxicated-mice
#20
Bharti Chaudhary, Renu Bist
The objective of the study was to evaluate the neuroprotective effects of bacoside A and bromelain against dichlorvos-incited toxicity. Healthy 6-8-week old, male Swiss mice were administered subacute doses of dichlorvos (40 mg/kg bw), bacoside A (5 mg/kg bw) and bromelain (70 mg/kg bw). AChE, BChE, GABA, serotonin and total protein content and their expressions were used for determination of toxic action of dichlorvos. Protective effects of bacoside A and bromelain were evaluated on the same parameters...
May 2017: Cell Stress & Chaperones
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