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https://www.readbyqxmd.com/read/28418600/validation-of-a-laboratory-developed-test-of-human-sperm-capacitation
#1
Melissa A Moody, Cristina Cardona, Alana J Simpson, T Timothy Smith, Alexander J Travis, G Charles Ostermeier
Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not respond to stimuli for capacitation. Sperm with "capacitated" patterns had exposed acrosomal carbohydrates and underwent acrosome exocytosis in response to calcium ionophore (A23187). Precision was evaluated by percent change of the Cap-Score measured for 50, 100, 150, and 200 sperm...
April 18, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28394630/plant-proteases-for-bioactive-peptides-release-a-review
#2
M A Mazorra-Manzano, J C Ramírez-Suarez, R Y Yada
Proteins are a potential source of health promoting biomolecules with medical, nutraceutical and food applications. Nowadays, bioactive peptides production, its isolation, characterization and strategies for its delivery to target sites are a matter of intensive research. In vitro and in vivo studies regarding the bioactivity of peptides has generated strong evidence of their health benefits. Dairy proteins are considered the richest source of bioactive peptides, however proteins from animal and vegetable origin also have been shown to be important sources...
April 10, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28392381/the-light-subunit-of-mushroom-agaricus-bisporus-tyrosinase-its-biological-characteristics-and-implications
#3
Wangsa T Ismaya, Olivia M Tandrasasmita, Syaripah Sundari, Diana, Xuelei Lai, Debbie S Retnoningrum, Bauke W Dijkstra, Raymond R Tjandrawinata, Heni Rachmawati
The light subunit of mushroom Agaricus bisporus tyrosinase (LSMT) is a protein of unknown function that was discovered serendipitously during the elucidation of the crystal structure of the enzyme. The protein is non-immunogenic and can penetrate the intestinal epithelial cell barrier, and thus, similar to its structural homologue HA-33 from Clostridium botulinum, may be potentially absorbable by the intestine. LSMT also shares high structural homology with the ricin-B-like lectin from the mushroom Clitocybe nebularis (CNL), which has been shown to display biological activity against leukemic cancer cells and dendritic cells...
April 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28363663/enzymatic-debridement-of-deeply-burned-faces-healing-and-early-scarring-based-on-tissue-preservation-compared-to-traditional-surgical-debridement
#4
Alexandra Schulz, Paul Christian Fuchs, Irene Rothermundt, Alexandra Hoffmann, Lior Rosenberg, Yaron Shoham, Henrik Oberländer, Jennifer Schiefer
INTRODUCTION: Facial burns occur frequently and depending on the injured skin layers often heal with scars which may cause permanent functional and cosmetic sequelae. Preservation of the sensitive facial skin layers, especially of the dermis is essential for scarless epithelialisation. Enzymatic debridement of deep thermal burns has already been shown to assist with preserving viable dermis. However, up to date, there are no published reports on wound healing and in the long term aesthetic outcome after enzymatic debridement of facial burns...
March 28, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28362352/optimization-of-bromelain-aided-production-of-angiotensin-i-converting-enzyme-inhibitory-hydrolysates-from-stone-fish-using-response-surface-methodology
#5
Shehu Muhammad Auwal, Mohammad Zarei, Azizah Abdul-Hamid, Nazamid Saari
The stone fish is an under-utilized sea cucumber with many nutritional and ethno-medicinal values. This study aimed to establish the conditions for its optimum hydrolysis with bromelain to generate angiotensin I-converting enzyme (ACE)-inhibitory hydrolysates. Response surface methodology (RSM) based on a central composite design was used to model and optimize the degree of hydrolysis (DH) and ACE-inhibitory activity. Process conditions including pH (4-7), temperature (40-70 °C), enzyme/substrate (E/S) ratio (0...
March 31, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28352546/bioactivities-of-fish-protein-hydrolysates-from-defatted-salmon-backbones
#6
Rasa Slizyte, Katariina Rommi, Revilija Mozuraityte, Peter Eck, Kathrine Five, Turid Rustad
Bioactivities of bulk fish protein hydrolysates (FPH) from defatted salmon backbones obtained with eight different commercial enzymes and their combinations were tested. All FPH showed antioxidative activity in vitro. DPPH scavenging activity increased, while iron chelating ability decreased with increasing time of hydrolysis. All FPH showed ACE inhibiting effect which depended on type of enzyme and increased with time of hydrolysis. The highest effect was found for FPH produced with Trypsin. Bromelain + Papain hydrolysates reduced the uptake of radiolabelled glucose into CaCo-2 cells, a model of human enterocytes, indicating a potential antidiabetic effect of FPH...
September 2016: Biotechnology Reports
https://www.readbyqxmd.com/read/28341257/our-initial-learning-curve-in-the-enzymatic-debridement-of-severely-burned-hands-management-and-pit-falls-of-initial-treatments-and-our-development-of-a-post-debridement-wound-treatment-algorithm
#7
A Schulz, W Perbix, Y Shoham, S Daali, C Charalampaki, P C Fuchs, J Schiefer
INTRODUCTION: Excisional surgical debridement (SD) is still the gold standard in the treatment of deeply burned hands, though the intricate anatomy is easily damaged. Previous studies demonstrated that enzymatic debridement with the bromelain debriding agent NexoBrid(®) (EDNX) is more selective and thus can preserve viable tissue with excellent outcome results. So far no method paper has been published presenting different treatment algorithms in this new field. Therefore our aim was to close this gap by presenting our detailed learning curve in EDNX of deeply burned hands...
March 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28321764/protective-manifestation-of-bacoside-a-and-bromelain-in-terms-of-cholinesterases-gamma-amino-butyric-acid-serotonin-level-and-stress-proteins-in-the-brain-of-dichlorvos-intoxicated-mice
#8
Bharti Chaudhary, Renu Bist
The objective of the study was to evaluate the neuroprotective effects of bacoside A and bromelain against dichlorvos-incited toxicity. Healthy 6-8-week old, male Swiss mice were administered subacute doses of dichlorvos (40 mg/kg bw), bacoside A (5 mg/kg bw) and bromelain (70 mg/kg bw). AChE, BChE, GABA, serotonin and total protein content and their expressions were used for determination of toxic action of dichlorvos. Protective effects of bacoside A and bromelain were evaluated on the same parameters...
March 20, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28261914/corrigendum-perioperative-bromelain-therapy-after-wisdom-teeth-extraction-a-randomized-placebo-controlled-double-blinded-three-armed-cross-over-dose-finding-study
#9
(no author information available yet)
No abstract text is available yet for this article.
March 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28247221/erratum-to-a-randomized-clinical-trial-to-evaluate-efficacy-and-tolerability-of-trypsin-chymotrypsin-as-compared-to-serratiopeptidase-and-trypsin-bromelain-rutoside-in-wound-management
#10
Ajay Chandanwale, Deepak Langade, Dheeraj Sonawane, Piyush Gavai
No abstract text is available yet for this article.
February 28, 2017: Advances in Therapy
https://www.readbyqxmd.com/read/28238440/the-proteolytic-system-of-pineapple-stems-revisited-purification-and-characterization-of-multiple-catalytically-active-forms
#11
André Matagne, Laetitia Bolle, Rachida El Mahyaoui, Danielle Baeyens-Volant, Mohamed Azarkan
Crude pineapple proteases extract (aka stem bromelain; EC 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. Numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterization. In this work, we describe an efficient strategy towards the purification of at least eight enzymatic forms...
February 23, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28217537/effect-of-proteolytic-enzyme-bromelain-on-pain-and-swelling-after-removal-of-third-molars
#12
Tejpal Singh, Vinayak More, Umayra Fatima, Tanveer Karpe, Mohammed A Aleem, J Prameela
AIMS AND OBJECTIVES: To evaluate the effect of bromelain on pain and swelling after surgical removal of third molars. MATERIALS AND METHODS: A prospective randomized clinical study was conducted among 40 patients requiring surgical removal of impacted mandibular third molars. All the impacted molars were surgically removed, and the patients were administered bromelain along with amoxicillin. Patients were recalled on day 1, day 3, and day 7 for postoperative pain assessment using visual analogue scale (VAS) and postoperative swelling assessment using facial reference points tragus and pogonion...
December 2016: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28215327/enzymes-in-fermented-fish
#13
Giyatmi, H E Irianto
Fermented fish products are very popular particularly in Southeast Asian countries. These products have unique characteristics, especially in terms of aroma, flavor, and texture developing during fermentation process. Proteolytic enzymes have a main role in hydrolyzing protein into simpler compounds. Fermentation process of fish relies both on naturally occurring enzymes (in the muscle or the intestinal tract) as well as bacteria. Fermented fish products processed using the whole fish show a different characteristic compared to those prepared from headed and gutted fish...
2017: Advances in Food and Nutrition Research
https://www.readbyqxmd.com/read/28205185/use-of-an-alpha-lipoic-methylsulfonylmethane-and-bromelain-dietary-supplement-opera-%C3%A2-for-chemotherapy-induced-peripheral-neuropathy-management-a-prospective-study
#14
Isacco Desideri, Giulio Francolini, Carlotta Becherini, Francesca Terziani, Camilla Delli Paoli, Emanuela Olmetto, Mauro Loi, Marco Perna, Icro Meattini, Vieri Scotti, Daniela Greto, Pierluigi Bonomo, Susanna Sulprizio, Lorenzo Livi
Chemotherapy-induced peripheral neuropathy (CIPN) is a major clinical problem associated with a number of cytotoxic agents. OPERA(®) (GAMFARMA srl, Milan, Italy) is a new dietary supplement where α-lipoic acid, Boswellia Serrata, methylsulfonylmethane and bromelain are combined in a single capsule. The aim of this prospective study was to determine the efficacy and safety of OPERA(®) supplementation in a series of patients affected by CIPN. We selected 25 subjects with CIPN evolving during or after chemotherapy with potentially neurotoxic agents...
March 2017: Medical Oncology
https://www.readbyqxmd.com/read/28196382/bromelain-loading-and-release-from-a-hydrogel-formulated-using-alginate-and-arabic-gum
#15
Janaína Artem Ataide, Letícia Caramori Cefali, Marcia de Araujo Rebelo, Lívia Genovez Spir, Elias Basile Tambourgi, Angela Faustino Jozala, Marco Vinícius Chaud, Edgar Silveira, Xiaochen Gu, Priscila Gava Mazzola
An ideal wound dressing ensures a moist environment around the wound area and absorbs exudates from the wound surface. Topical application of bromelain to incised wounds has been shown to reprogram the wound microenvironment to promote effective tissue repair. Combining the characteristics of hydrogels and bromelain is therefore of great interest. Herein, we describe the development of a hydrogel, formulated using alginate and Arabic gum, for bromelain loading and release. The hydrogel formulation was evaluated using response surface methodology, considering the pH value and the concentration of alginate and Arabic gum...
February 14, 2017: Planta Medica
https://www.readbyqxmd.com/read/28181300/synthesis-and-characterization-of-cefotaxime-conjugated-gold-nanoparticles-and-their-use-to-target-drug-resistant-ctx-m-producing-bacterial-pathogens
#16
Sibhghatulla Shaikh, Syed Mohd Danish Rizvi, Shazi Shakil, Talib Hussain, Thamir M Alshammari, Waseem Ahmad, Shams Tabrez, Mohammad H Al-Qahtani, Adel M Abuzenadah
Multidrug-resistance due to 'β lactamases having the expanded spectrum' (ESBLs) in members of Enterobacteriaceae is a matter of continued clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant bacterial strains (i.e. one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of 'cefotaxime loaded gold-nanoparticles'...
February 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28157418/whitening-efficacy-of-whitening-mouth-rinses-used-alone-or-in-conjunction-with-carbamide-peroxide-home-whitening
#17
Jbs Oliveira, R S Sarlo, E Bresciani, Tmf Caneppele
OBJECTIVES: The aim of this study was to compare the effectiveness of whitening mouth rinses on teeth previously whitened or not, exposed to food dyes. METHODS AND MATERIALS: One hundred twenty enamel-dentin specimens, 3 mm in diameter, were obtained from bovine incisors. The specimens were stained for 14 days in staining broth. After staining, the initial color reading was performed via a spectrophotometer CM-2600d (Konica Minolta). Half of specimens were submitted to whitening (10% carbamide peroxide [CP]) for 14 days...
February 3, 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28154803/involvement-of-cross-reactive-carbohydrate-determinants-specific-ige-in-pollen-allergy-testing
#18
Hidenori Yokoi, Hiroshi Yoshitake, Yuma Matsumoto, Michitsugu Kawada, Yoshiki Takato, Kiyomi Shinagawa, Hiroyuki Sakurai, Koichiro Saito
BACKGROUND: Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive results for grain or legume allergens can be reported for pollen allergic patients who are not sensitized to those allergens...
January 2017: Asia Pacific Allergy
https://www.readbyqxmd.com/read/28138305/physical-and-chemical-characteristics-of-mucin-secreted-by-pseudomyxoma-peritonei-pmp
#19
Krishna Pillai, Javed Akhter, Ahmed Mekkawy, Terence C Chua, David L Morris
Background: Pseudomyxoma peritonei (PMP) is a rare disease with excess intraperitoneal mucin secretion. Treatment involves laparotomy, cytoreduction and chemotherapy that is very invasive with patients often acquiring numerous compromises. Hence a mucolytic comprising of bromelain and N-acetyl cystein has been developed to solubilise mucin in situ for removal by catherization. Owing to differences in mucin appearance and hardness, dissolution varies. Therefore the current study investigates the inter-mucin physical and chemical characteristics, in order to reformulate an effective mucolytic for all mucin...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28135202/using-a-radial-diffusion-method-to-investigate-the-role-of-plasmin-degradation-of-fibrin-in-a-physical-model-of-an-early-phase-wound
#20
Chima P Chukwuemeka, Jann Cupp, Jonathan R Sanders
BACKGROUND: Fibrin clot formation, which acts to stabilize a wound following injury, is among the key early aspects of dermal wound healing. This preliminary matrix is eventually degraded via a process known as fibrinolysis and replaced with a collagen-rich matrix that continues to be remodeled to minimize scarring. Disruptions in these carefully coordinated events lead to certain undesirable conditions such as fibrosis and the formation of abnormal scars that are associated with excess amounts of collagen...
January 23, 2017: Wounds: a Compendium of Clinical Research and Practice
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