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Casein protein

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https://www.readbyqxmd.com/read/29778479/an-in-vivo-stable-isotope-based-approach-for-assessment-of-absorbed-amino-acids-from-individual-feed-ingredients-within-complete-diets
#1
K A Estes, R R White, P S Yoder, T Pilonero, H Schramm, H Lapierre, M D Hanigan
Accurate assessment of the nutritional content of feed ingredients is required for precise diet formulation. Characterizing ingredients in terms of absorption and digestibility of individual AA is challenging, and this information often relies on indirect methods. The purpose of this research was to evaluate an in vivo stable isotope-based method of determining plasma entry rates of individual AA from feather meal (FM), blood meal (BM), and a rumen-protected AA (RPMet). Abomasal infusions of unprotected Ile, Leu, Met, and sodium caseinate were used as control treatments to assess technique reliability and accuracy...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29778477/the-effects-of-microfluidization-on-the-physical-microbial-chemical-and-coagulation-properties-of-milk
#2
A J Bucci, D L Van Hekken, M H Tunick, J A Renye, P M Tomasula
This work examines the use of mild heat treatments in conjunction with 2-pass microfluidization to generate cheese milk for potential use in soft cheeses, such as Queso Fresco. Raw, thermized, and high temperature, short time pasteurized milk samples, standardized to the 3% (wt/wt) fat content used in cheesemaking, were processed at 4 inlet temperature and pressure conditions: 42°C/75 MPa, 42°C/125 MPa, 54°C/125 MPa, and 54°C/170 MPa. Processing-induced changes in the physical, chemical, and microbial properties resulting from the intense pressure, shear, and cavitation that milk experiences as it is microfluidized were compared with nonmicrofluidized controls...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29774078/targeting-intracellular-mmps-efficiently-inhibits-tumor-metastasis-and-angiogenesis
#3
Yaqi Lv, Xiangmei Zhao, Lidan Zhu, Sijia Li, Qingqing Xiao, Wei He, Lifang Yin
Treatment for metastatic cancer is a great challenge throughout the world. Commonly, directed inhibition of extracellular matrix metalloproteinases (MMPs) secreted by cancer cells can reduce metastasis. Here, a novel nanoplatform (HPMC NPs) assembled from hyaluronic acid (HA)-paclitaxel (PTX) prodrug and marimastat (MATT)/β-casein (CN) complexes was established to cure a 4T1 metastatic cancer model via targeting CD44 and intracellular, rather than extracellular, MMPs. Methods: HPMC NPs were prepared by assembling the complexes and prodrug under ultrasonic treatment, which the interaction between them was evaluated by förster resonance energy transfer, circular dichroism and fluorescence spectra...
2018: Theranostics
https://www.readbyqxmd.com/read/29772827/physicochemical-nutritional-and-organoleptic-characterization-of-a-skimmed-goat-milk-fermented-with-the-probiotic-strain-lactobacillus-plantarum-c4
#4
Miriam Moreno-Montoro, Miguel Navarro-Alarcón, Triana Bergillos-Meca, Rafael Giménez-Martínez, Silvia Sánchez-Hernández, Manuel Olalla-Herrera
The benefits of goat milk, fermented milks, and probiotics for the humans are well documented. In this study, a novel fermented goat milk was manufactured with the putative probiotic strain Lactobacillus plantarum C4 together with L. bulgaricus and Streptococcus thermophilus . Ultrafiltration was chosen as the skimmed milk concentration method because it produced the best viscosity and syneresis and a high casein content. The viability rate of all bacterial strains was >10⁷ cfu/mL, even after 5 weeks of storage or after in vitro gastrointestinal digestion, which is especially important for exertion of the probiotic strain functionalities...
May 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29770757/soya-protein-%C3%AE-conglycinin-ameliorates-fatty-liver-and-obesity-in-diet-induced-obese-mice-through-the-down-regulation-of-ppar%C3%AE
#5
Dongyang Li, Reina Ikaga, Tomomi Yamazaki
Diets high in fat can result in obesity and non-alcoholic fatty liver disease (NAFLD). The improvement of obesity and NAFLD is an important issue. β-Conglycinin, one of the soya proteins, is known to prevent hyperlipidaemia, obesity and NAFLD. Therefore, we aimed to investigate the effects of β-conglycinin on the improvement of obesity and NAFLD in high-fat (HF) diet-induced obese (DIO) mice and clarify the mechanism underlying these effects in liver and white adipose tissue (WAT). DIO male ddY mice were divided into six groups: HF, medium-fat (MF) and low-fat (LF) groups fed casein, and HF, MF and LF groups in all of which the casein was replaced by β-conglycinin...
June 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29767155/responses-of-milk-production-of-dairy-cows-to-jugular-infusions-of-a-mixture-of-essential-amino-acids-with-or-without-exclusion-leucine-or-arginine
#6
Wen Tian, Tianyou Wu, Rui Zhao, Jinhao Xu, Yao He, Hongrong Wang
The purpose of this study was to determine effects of jugular infusion of either balanced or imbalanced amino acid mixture on milk production and composition in dairy cows. Eight mid-lactation Holstein cows were randomly assigned to 5-d continuous jugular infusions of saline (CTL), essential amino acid (EAA) mixture prepared on the profile of casein (CSN, 160 g/d), EAA mixture excluding leucine (Leu) (-Leu, 163 g/d) or EAA mixture excluding arginine (Arg) (-Arg, 158 g/d) in a duplicated 4 × 4 Latin square design with 4 infusion periods separated by a 7-d interval period...
September 2017: Animal nutrition
https://www.readbyqxmd.com/read/29767145/an-increase-in-corn-resistant-starch-decreases-protein-fermentation-and-modulates-gut-microbiota-during-in-vitro-cultivation-of-pig-large-intestinal-inocula
#7
Xiangyu He, Weiwei Sun, Ting Ge, Chunlong Mu, Weiyun Zhu
High-protein diet could cause an increase in protein fermentation in the large intestine, leading to an increased production of potentially detrimental metabolites. We hypothesized that an increase in corn resistant starch content may attenuate the protein fermentation. The aim of this study was to evaluate the effect of resistant starch on protein fermentation by inocula from large intestine of pigs using in vitro cultivation. Fermentation patterns were analyzed during a 24-h incubation of cecal and colonic digesta with varying corn resistant starch contents, using casein protein as sole nitrogen source...
September 2017: Animal nutrition
https://www.readbyqxmd.com/read/29764624/hydrolyzed-proteins-from-herring-and-salmon-rest-raw-material-contain-peptide-motifs-with-angiotensin-i-converting-enzyme-inhibitors-and-resulted-in-lower-urine-concentrations-of-protein-cystatin-c-and-glucose-when-fed-to-obese-zucker-fa-fa-rats
#8
Aslaug Drotningsvik, Daniela Maria Pampanin, Rasa Slizyte, Ana Carvajal, Ingmar Høgøy, Tore Remman, Oddrun Anita Gudbrandsen
The use of angiotensin-I converting enzyme (ACE) inhibitors is a common strategy for treating kidney disease. Several amino acid sequences with ACE inhibiting activity are identified in filet and rest raw material from various species of fish, and fish protein hydrolysates could be of interest for possible treatment or prevention of kidney disease. Therefore, we hypothesized that protein hydrolysates from rest raw material from herring and salmon contained ACE inhibiting motifs, and could beneficially affect typical markers for kidney function in an obesity rat model prone to developing renal failure...
April 2018: Nutrition Research
https://www.readbyqxmd.com/read/29764464/daytime-and-nighttime-casein-supplements-similarly-increase-muscle-size-and-strength-in-response-to-resistance-training-earlier-in-the-day-a-preliminary-investigation
#9
Jordan M Joy, Roxanne M Vogel, K Shane Broughton, Urszula Kudla, Nathaniel Y Kerr, Jason M Davison, Robert E C Wildman, Nancy M DiMarco
BACKGROUND: Casein protein consumed before sleep has been suggested to offer an overnight supply of exogenous amino acids for anabolic processes. The purpose of this study was to compare supplemental casein consumed earlier in the day (DayTime, DT) versus shortly before bed (NightTime, NT) on body composition, strength, and muscle hypertrophy in response to supervised resistance training. METHODS: Thirteen males participated in a 10-week exercise and dietary intervention while receiving 35 g casein daily...
May 15, 2018: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/29763383/eb-2017-article-soy-protein-isolate-feeding-does-not-result-in-reproductive-toxicity-in-the-pre-pubertal-rat-testis
#10
Martin Jj Ronis, Horacio Gomez-Acevedo, Kartik Shankar, Neha Sharma, Michael Blackburn, Rohit Singhal, Kelly E Mercer, Thomas M Badger
The isoflavone phytoestrogens found in the soy protein isolate used in soy infant formulas have been shown to have estrogenic actions in the developing male reproductive tract resulting in reproductive toxicity. However, few studies have examined potential estrogenicity of soy protein isolate as opposed to that of pure isoflavones. In this study, we fed weanling male Sprague-Dawley rats a semi-purified diet with casein or soy protein isolate as the sole protein source from postnatal day 21 to 33. Additional groups were fed casein or soy protein isolate and treated s...
May 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29757972/short-term-soy-protein-isolate-feeding-prevents-liver-steatosis-and-reduces-serum-alt-and-ast-levels-in-obese-female-zucker-rats
#11
Reza Hakkak, Clinton Heath Gauss, Andrea Bell, Soheila Korourian
Non-alcoholic fatty liver disease is a common liver disorder worldwide and is associated with obesity. We investigated effects of obesity and short-term intake of soy protein with isoflavones (SPI) on body weight change, energy intake, liver steatosis, and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and leptin levels. Seventeen lean and seventeen obese ( fa / fa ) female Zucker rats were randomly assigned to either casein or SPI diet for 8 weeks. Body weight was recorded twice weekly; feed intake was measured weekly...
May 14, 2018: Biomedicines
https://www.readbyqxmd.com/read/29756217/comparative-two-dimensional-polyacrylamide-gel-electrophoresis-2d-page-of-human-milk-to-identify-dysregulated-proteins-in-breast-cancer
#12
Roshanak Aslebagh, Devika Channaveerappa, Kathleen F Arcaro, Costel C Darie
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of a pilot proteomics study, using one-dimensional polyacrylamide gel electrophoresis (1D-PAGE) and mass spectrometry (MS) to investigate differences in milk proteins from women with and without BC. Here, we applied two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MS to compare the protein pattern in milk from the breasts of a single woman who was diagnosed with BC in one breast 24 months after donating her milk...
May 13, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29753488/gastric-digestion-of-milk-protein-ingredients-study-using-an-in-vitro-dynamic-model
#13
Xin Wang, Aiqian Ye, Quanquan Lin, Jianzhong Han, Harjinder Singh
The coagulation behavior and the kinetics of protein hydrolysis of skim milk powder, milk protein concentrate (MPC), calcium-depleted MPC, sodium caseinate, whey protein isolate (WPI), and heated (90°C, 20 min) WPI under gastric conditions were examined using an advanced dynamic digestion model (i.e., a human gastric simulator). During gastric digestion, these protein ingredients exhibited various pH profiles as a function of the digestion time. Skim milk powder and MPC, which contained casein micelles, formed cohesive, ball-like curds with a dense structure after 10 min of digestion; these curds did not disintegrate over 220 min of digestion...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753479/production-of-calcium-and-magnesium-enriched-caseins-and-caseinates-by-an-ecofriendly-technology
#14
Félix-André Masson, Sergey Mikhaylin, Laurent Bazinet
Finding new green ways of producing proteins has never been of such critical public interest, both to meet consumers' needs and to preserve the environment. Milk proteins are among the most attractive protein types due to their high nutritional value and attractive functional properties. In this work, the separation of caseins by conventional chemical acidification was compared with electrodialysis with bipolar membrane coupled to an ultrafiltration module (EDBM-UF), a green process that allows the precipitation of caseins by H+ generated in situ by the bipolar membrane and, simultaneously, the production of a separated NaOH stream from OH- electrogenerated by the bipolar membrane...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753472/investigating-the-properties-of-high-pressure-treated-reduced-sodium-low-moisture-part-skim-mozzarella-cheese-during-refrigerated-storage
#15
M Ozturk, S Govindasamy-Lucey, J J Jaeggi, M E Johnson, J A Lucey
We proposed that the performance and sensory properties of reduced-Na, low-moisture, part-skim (LMPS) Mozzarella cheese could be extended by the application of high hydrostatic pressure (HHP) to cheese postmanufacture and thereby decrease microbial and enzymatic activity. Fermentation-produced camel chymosin was also used as a coagulant to help reduce proteolysis during storage. Average composition of the LMPS Mozzarella cheeses was 48.6 ± 0.6% moisture, 22.5 ± 0.4% fat, 24.5 ± 0.6% protein, and 1.0 ± 0...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753471/effects-of-processing-conditions-on-the-texture-and-rheological-properties-of-model-acid-gels-and-cream-cheese
#16
M Brighenti, S Govindasamy-Lucey, J J Jaeggi, M E Johnson, J A Lucey
Manufacture of cream cheese involves the formation of an initial acid-induced gel made from high-fat milk, followed by a series of processing steps including shearing, heating, and dewatering that complete the conversion of the acid gel into a complex cheese product. We investigated 2 critical parameters for their effect on the initial gel: homogenization pressure (HP) of the high-fat cheese milk, and fermentation temperature (FT). The impact of a low (10 MPa) and high (25 MPa) HP, and low (20°C) and high (26°C) FT were investigated for their effects on rheological and textural properties of acid-induced gels...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753426/-sardine-purified-proteins-improve-blood-pressure-glycemic-control-anti-atherogenic-metabolic-pathways-and-antioxidant-capacity-in-obese-rats
#17
F Affane, F Bensalah, N I Harrat, H Chekkal, S Louala, M Y Lamri-Senhadji
AIM OF THE STUDY: The effects of sardine by-products (SBy-P) and fillet proteins (SF-P) were compared to casein (Cas) ; these effects were assessed on blood pressure, glycemic control, reverse cholesterol transport, lipid peroxidation and total antioxidant capacity in obese rats. MATERIALS AND METHODS: Eighteen male Wistar rats were subjected for three months, to a high-fat diet. The obese rats were divided into three groups and consumed the same high-fat diet for 28 days after addition of either, 20% SBy-P, SF-P or Cas...
May 9, 2018: Annales de Cardiologie et D'angéiologie
https://www.readbyqxmd.com/read/29746701/nitrogen-retention-energy-and-amino-acid-digestibility-of-wheat-bran-without-or-with-multicarbohydrase-and-phytase-supplementation-fed-to-broiler-chickens
#18
Connie Gallardo, Julio Cezar Dadalt, Messias Alves Trindade Neto
The study was conducted to determine the effects of multicarbohydrase (MC) preparation (700 U α-galactosidase, 2,200 U galactomannanase, 3,000 U xylanase, and 22,000 U β-glucanase per kg of diet) and phytase (Phy, 500 FTU per kg of diet) supplementation on the nutritive value of wheat bran (WB) in broiler chicks. Trial 1 determined retention of nutrients and apparent metabolizable energy corrected by nitrogen (AMEn). One reference diet (RD) protein-free (85% corn based) was fortified to determine the WB nutrient retention coefficient...
May 8, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29746547/combining-different-proteomic-approaches-to-resolve-complexity-of-the-milk-protein-fraction-of-dromedary-bactrian-camels-and-hybrids-from-different-regions-of-kazakhstan
#19
Alma Ryskaliyeva, Céline Henry, Guy Miranda, Bernard Faye, Gaukhar Konuspayeva, Patrice Martin
Nutritional suitability of milk is not only related to gross composition, but is also strongly affected by the microheterogeniety of the protein fraction. Hence, to go further into the evaluation of the potential suitability of non-bovine milks in human/infant nutrition it is necessary to have a detailed characterization of their protein components. Combining proven proteomic approaches (SDS-PAGE, LC-MS/MS and LC-ESI-MS) and cDNA sequencing, we provide here in depth characterization of the milk protein fraction of dromedary and Bactrian camels, and their hybrids, from different regions of Kazakhstan...
2018: PloS One
https://www.readbyqxmd.com/read/29745984/effects-of-processing-on-structure-and-thermal-properties-of-powdered-preterm-infant-formula
#20
Xiaomeng Sun, Cuina Wang, Hao Wang, Mingruo Guo
Powdered infant formula is usually manufactured by ingredients mixing, homogenization, pasteurization, evaporation and spray drying. Effects of unit operations on the microstructure, thermal properties and other characteristics of preterm infant formula, fat (F), serum (S), and pellet (P) fractions on centrifugation were investigated using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Differential Scanning Calorimetry (DSC), and Fourier Transform Infrared (FTIR) spectroscopy. After homogenization, particles which may be casein and denatured whey proteins were observed on the surface of F fraction in microstructure images...
May 10, 2018: Journal of Food Science
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