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https://www.readbyqxmd.com/read/28527412/early-stage-detection-of-ve-cadherin-during-endothelial-differentiation-of-human-mesenchymal-stem-cells-using-spr-biosensor
#1
Farzaneh Fathi, Aysa Rezabakhsh, Reza Rahbarghazi, Mohammad-Reza Rashidi
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. Up to present, SPR application in stem cell biology and biomedical sciences was underused. Herein, a very simple and sensitive method was developed to evaluate human mesenchymal stem cells trans-differentiation to endothelial lineage of over a period of 14 days based on VE-cadherin biomarker...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28527361/preparation-and-antifouling-properties-of-2-meth-acryloyloxy-ethyl-cholinephosphate-based-polymers-modified-surface-with-different-molecular-architectures-by-atrp
#2
Yuchen Jiang, Yuling Su, Lili Zhao, Fancui Meng, Quanxin Wang, Chunmei Ding, Jianbin Luo, Jianshu Li
Choline phosphate (CP) containing polymers modified surfaces have been shown good resist to the adhesion of proteins while prompt the attaching of mammalian cells due to the dipole pairing between the CP groups of the polymer and the phosphorylcholine (PC) groups on the cell membrane. However, the antifouling activities of CP modified surface against microbes have not been investigated at present. In addition, CP containing polymers modified surface with different molecular architectures has not been prepared and studied...
May 11, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28527192/in-vitro-evaluation-of-an-injectable-biphasic-calcium-phosphate-bcp-carrier-system-combined-with-recombinant-human-bone-morphogenetic-protein-rhbmp-9
#3
Masako Fujioka-Kobayashi, Benoit Schaller, Yufeng Zhang, Benjamin E Pippenger, Richard J Miron
Bone morphogenetic protein 9 (BMP9) has previously been characterized as the strongest osteoinductive growth factor among the BMP family. The aim of the present study was to evaluate the possibility of combining rhBMP9 with an injectable biphasic calcium phosphate (I-BCP, maxresorb inject®), since I-BCP is an easy to handle biomaterial with ideal properties for bone augmentation procedures. The adsorption potential of rhBMP9 as well as the cell behavior of bone stromal ST2 cells were investigated on cell viability, adhesion, proliferation and osteogenic differentiation for I-BCP combined with/without rhBMP9 in vitro...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28527188/optimum-pure-intrusion-of-a-mandibular-canine-with-the-segmented-arch-in-lingual-orthodontics
#4
Abhishek M Thote, Krishna Sharma, Rashmi V Uddanwadiker, Sunita Shrivastava
BACKGROUND: Approximately 50% patients with a deep bite possess anatomically extruded mandibular canines. OBJECTIVE: The objective of this study was to specify an optimum toe (Θ) of the vertical segment of a cantilever from the distal aspect to achieve pure intrusion of a mandibular canine with the segmented arch in lingual orthodontics (LiO). METHODS: The geometrical model of a mandibular canine was developed to compute the required values of Θ (positive values - toe-in, negative values - toe-out)...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28527185/surface-heparinization-and-blood-compatibility-modification-of-small-intestinal-submucosa-sis-for-small-caliber-vascular-regeneration
#5
Bensong Han, Feng Xue, Cunyi Fan, Xiumei Mo
OBJECTIVES: This study aims to investigate the small intestinal submucosal (SIS) surface after heparinization with the hypothermia plasma technique, to improve the blood compatibility of SIS, and to explore the possibility of construction of small-caliber vascular grafts with modified SIS scaffolds in vivo. METHODS: SIS films prepared from jejunums of pigs were processed for surface treatment at different time periods with the argon plasma initiation technique under vacuum, and were then immediately immersed in 4% (m/v) heparin sodium solution for 24-h heparinization...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28527006/analysis-of-surgical-and-mri-factors-associated-with-cerebellar-mutism
#6
Anjali Sergeant, Michelle Masayo Kameda-Smith, Branavan Manoranjan, Brij Karmur, JoAnn Duckworth, Tina Petrelli, Katey Savage, Olufemi Ajani, Blake Yarascavitch, M Constantine Samaan, Katrin Scheinemann, Cheryl Alyman, Saleh Almenawer, Forough Farrokhyar, Adam J Fleming, Sheila Kumari Singh, Nina Stein
The surgical risk factors and neuro-imaging characteristics associated with cerebellar mutism (CM) remain unclear and require further investigation. Therefore, we aimed to examine surgical and MRI findings associated with CM in children following posterior fossa tumor resection. Using our data registry, we retrospectively collected data from pediatric patients who acquired CM and were matched based on age and pathology type with individuals who did not acquire CM after posterior fossa surgery. The strength of association between surgical and MRI variables and CM were examined using odds ratios (ORs) and corresponding 95% confidence intervals (CIs)...
May 19, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28526794/raffinose-induces-biofilm-formation-by-streptococcus-mutans-in-low-concentrations-of-sucrose-by-increasing-production-of-extracellular-dna-and-fructan
#7
Ryo Nagasawa, Tsutomu Sato, Hidenobu Senpuku
Streptococcus mutans is the primary etiological agent of dental caries and causes tooth decay by forming a firmly attached biofilm on tooth surfaces. Biofilm formation is induced by the presence of sucrose, which is a substrate for the synthesis of extracellular polysaccharides, but not in the presence of oligosaccharides. Nonetheless, in this study, we found that raffinose, which is an oligosaccharide with an intestinal regulatory function and antiallergic effect, induced biofilm formation by S. mutans in a mixed culture with sucrose which was at concentrations less than those required to induce biofilm formation directly...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526791/pseudomonas-chlororaphis-produces-two-distinct-r-tailocins-that-contribute-to-bacterial-competition-in-biofilms-and-on-roots
#8
Robert J Dorosky, Jun Myoung Yu, Leland S Pierson, Elizabeth A Pierson
R-type tailocins are high molecular weight bacteriocins that resemble bacteriophage tails and are encoded within the genomes of many Pseudomonas species. In this study, analysis of the P. chlororaphis 30-84 R-tailocin gene cluster revealed that it encodes the structural components to produce two R-tailocins of different ancestral origin. Two distinct R-tailocin populations differing in length were observed in UV induced lysates of P. chlororaphis 30-84 via transmission electron microscopy. Mutants defective in the production of one or both R-tailocins demonstrated that the killing spectrum of each tailocin is limited to Pseudomonas species...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526785/effects-of-arginine-on-growth-virulence-gene-expression-and-stress-tolerance-by-streptococcus-mutans
#9
Brinta Chakraborty, Robert A Burne
Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In contrast, many commensal bacteria produce ammonia through the arginine deiminase system (ADS), which moderates the pH of oral biofilms. Arginine metabolism by the ADS is a significant deterrent to the initiation and progression of dental caries...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526662/attachment-style-and-internet-addiction-an-online-survey
#10
Christiane Eichenberg, Markus Schott, Oliver Decker, Brigitte Sindelar
BACKGROUND: One of the clinically relevant problems of Internet use is the phenomenon of Internet addiction. Considering the fact that there is ample evidence for the relationship between attachment style and substance abuse, it stands to reason that attachment theory can also make an important contribution to the understanding of the pathogenesis of Internet addiction. OBJECTIVE: The aim of this study was to examine people's tendency toward pathological Internet usage in relation to their attachment style...
May 17, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28526238/ric8a-is-essential-for-the-organisation-of-actin-cytoskeleton-and-cell-matrix-interaction
#11
Katrin Ruisu, Riho Meier, Keiu Kask, Tambet Tõnissoo, Teet Velling, Margus Pooga
RIC8A functions as a chaperone and guanine nucleotide exchange factor for a subset of G protein α subunits. Multiple G protein subunits mediate various signalling events that regulate cell adhesion and migration and the involvement of RIC8A in some of these processes has been demonstrated. We have previously shown that the deficiency of RIC8A causes a failure in mouse gastrulation and neurogenesis - major events in embryogenesis that rely on proper association of cells with the extracellular matrix (ECM) and involve active cell migration...
May 16, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28526091/a-pre-therapeutic-coating-for-medical-devices-that-prevents-the-attachment-of-candida-albicans
#12
Diego Vargas-Blanco, Aung Lynn, Jonah Rosch, Rony Noreldin, Anthony Salerni, Christopher Lambert, Reeta P Rao
BACKGROUND: Hospital acquired fungal infections are defined as "never events"-medical errors that should never have happened. Systemic Candida albicans infections results in 30-50% mortality rates. Typically, adhesion to abiotic medical devices and implants initiates such infections. Efficient adhesion initiates formation of aggressive biofilms that are difficult to treat. Therefore, inhibitors of adhesion are important for drug development and likely to have a broad spectrum efficacy against many fungal pathogens...
May 19, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28526085/papillary-fibroelastoma-unusual-cause-of-stroke-in-a-young-man-a-case-report
#13
Elisabetta Grolla, Michele Dalla Vestra, Giampaolo Zoffoli, Riccardo D'Ascoli, Adriana Critelli, Rocco Quatrale, Domenico Mangino, Fausto Rigo
BACKGROUND: Papillary fibroelastoma is the third most common primary benign tumor with an incidence of up to 0.33% in autopsy series; it accounts for approximately 75% of all cardiac valvular tumors. CASE PRESENTATION: We describe a rare case of a 28-Year-old man that while playing football, had a sudden onset of neurological deficit: aphasia, right hemiparesis and right facial numbness. Transthoracic echocardiography (TTE) showed a 10x10 mm mass attached to the anterior mitral valve leaflet...
May 19, 2017: Journal of Cardiothoracic Surgery
https://www.readbyqxmd.com/read/28525848/an-amplified-comparative-fluorescence-resonance-energy-transfer-immunosensing-of-ca125-tumor-marker-and-ovarian-cancer-cells-using-green-and-economic-carbon-dots-for-bio-applications-in-labeling-imaging-and-sensing
#14
Somayeh Hamd-Ghadareh, Abdollah Salimi, Fardin Fathi, Saman Bahrami
CA125, is a marker in the clinical diagnosis of several cancers and currently is the best serum-based tumor marker for ovarian cancer. Here, we developed an ultrasensitive antibody-ssDNA aptamer sandwich-type fluorescence immunosensor for CA125 detection. Based on a novel signal amplification strategy the carbon dots (CDs) functionalized with aptamer (CD-aptamer) used as detection probe and PAMAM-Dendrimers/AuNPs was used for covalent attachment of CA125-antibody and completing the sandwich assay method. By measuring of fluorescence resonance energy transfer (FRET) signals between CDs and AuNPs as nanoquenchers, the fluorescence signal quenched during sandwich complex formed between anti-CA125, CA125 and CDs-Aptamer and decreasing of fluorescence response signal is related to CA125 concentrations...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525783/toolset-for-assessment-of-natural-recovery-from-legacy-contaminated-sediment-case-study-of-pallanza-bay-lake-maggiore-italy
#15
Diana Lin, Yeo-Myoung Cho, Amy Oen, Espen Eek, Jake P Tommerdahl, Richard G Luthy
The aim of this study was to develop a toolset that can be used by site managers to assess and monitor natural attenuation processes in sediments contaminated with legacy hydrophobic organic contaminants. The toolset is composed of sediment traps to measure quality and deposition rate of incoming sediment under different hydrodynamic conditions, sediment cores to show trends in sediment bed concentrations over time, and passive samplers attached to a porewater probe frame to assess the mobility of buried contaminants and possible contaminant flux from sediment...
May 12, 2017: Water Research
https://www.readbyqxmd.com/read/28525718/on-the-stability-of-the-parent-anion-of-the-potential-radiosensitizer-8-bromoadenine-formed-by-low-energy-3-ev-electron-attachment
#16
Robin Schürmann, Katrin Tanzer, Iwona Dabkowska, Stephan Paul Denifl, Ilko Bald
8-Bromoadenine (8BrA) is a potential DNA radiosensitizer for cancer radiation therapy due to its efficient interaction with low energy electrons (LEEs). LEEs are a short-living species generated during the radiation damage of DNA by high energy radiation as it is applied in cancer radiation therapy. Electron attachment to 8BrA in the gas phase results in a stable parent anion below 3 eV electron energy in addition to fragmentation products formed by resonant exocyclic bond cleavages. Density functional theory (DFT) calculations of the 8BrA- anion reveal an exotic bond between the bromine and the C8 atom with a bond length of 2...
May 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28525708/affinity-based-assembly-of-peptides-on-plasmonic-nanoparticles-delivered-intracellularly-with-light-activated-control
#17
Demosthenes P Morales, William Wonderly, Xiao Huang, Meghan McAdams, Amanda Chron, Norbert O Reich
We report a universal strategy for functionalizing near-infrared light-responsive nanocarriers with both a peptide "cargo" and an orthogonal cell-penetrating peptide. Modularity of both the cargo and internalization peptide attachment are an important feature of these materials relying on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of nickel as well as the affinity of biotin labeled peptides to streptavidin. Attachment to the gold surface uses thiol labeled scaffolds terminated with the affinity partner...
May 19, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28525466/facet-preserving-technique-by-en-bloc-flavectomy-in-microscopic-posterior-decompression-surgery-for-lumbar-spinal-stenosis-semicircumferential-decompression-scd
#18
Yasushi Fujiwara, Hideki Manabe, Tadayoshi Sumida, Bunichiro Izumi, Kazuyoshi Nakanishi, Nobuhiro Tanaka, Nobuo Adachi
Posterior decompression surgery which enlarges the spinal canal in cases with lumbar spinal stenosis is the most fundamental technique in spine surgery, but controversy still remains for the exact technique. Because nerve roots run under the ventral side on the superior articular process (SAP), achieving both facet joint preservation and nerve root decompression is problematic. In conventional laminotomies or laminectomies, medial facetectomies are performed to decompress the nerve root, but it is ideal to preserve the facet joints completely for preserving joint stability...
June 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28525314/malaria-parasite-limp-protein-regulates-sporozoite-gliding-motility-and-infectivity-in-mosquito-and-mammalian-hosts
#19
Jorge M Santos, Saskia Egarter, Vanessa Zuzarte-Luís, Hirdesh Kumar, Catherine A Moreau, Jessica Kehrer, Andreia Pinto, Mário da Costa, Blandine Franke-Fayard, Chris J Janse, Friedrich Frischknecht, Gunnar R Mair
Gliding motility allows malaria parasites to migrate and invade tissues and cells in different hosts. It requires parasite surface proteins to provide attachment to host cells and extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers to a gliding phenotype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion during gliding motility in the rodent malaria model P. berghei. Transcribed in gametocytes, LIMP is translated in the ookinete from maternal mRNA, and later in the sporozoite...
May 2, 2017: ELife
https://www.readbyqxmd.com/read/28525281/interaction-of-7-alkoxycoumarins-with-the-aryl-hydrocarbon-receptor
#20
Marco Gargaro, Francesco Epifano, Serena Fiorito, Vito Alessandro Taddeo, Salvatore Genovese, Matteo Pirro, Antonella Turco, Paolo Puccetti, Carsten B Schmidt-Weber, Francesca Fallarino
The aryl hydrocarbon receptor (AhR) is a transcription factor activated by a vast array of natural and synthetic ligands. It plays a pivotal role in numerous physiological and pathological responses, such as cell proliferation and differentiation, induction of xenobiotic metabolizing enzymes, response to environmental toxins, and several others. In this study, we investigated the ability of some natural compounds (oxyprenylated ferulic acid and umbelliferone derivatives) and their semisynthetic analogues (e...
May 19, 2017: Journal of Natural Products
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