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https://www.readbyqxmd.com/read/28213963/ultrastructure-of-compatible-and-incompatible-interactions-in-phloem-sieve-elements-during-the-stylet-penetration-by-cotton-aphids-in-melon
#1
Elisa Garzo, Mercedes Fernández-Pascual, Cesar Morcillo, Alberto Fereres, M Luisa Gómez-Guillamón, W Fred Tjallingii
Resistance of the melon line TGR-1551 to the aphid Aphis gossypii is based on preventing aphids from ingesting phloem sap. In electrical penetration graphs (EPGs), this resistance has been characterized with A. gossypii showing unusually long phloem salivation periods (waveform E1) mostly followed by pathway activities (waveform C) or if followed by phloem ingestion (waveform E2), ingestion was not sustained for more than 10 minutes. Stylectomy with aphids on susceptible and resistant plants was performed during EPG recording while the stylet tips were phloem inserted...
February 18, 2017: Insect Science
https://www.readbyqxmd.com/read/28212656/using-a-vector-pool-containing-variable-strength-promoters-to-optimize-protein-production-in-yarrowia-lipolytica
#2
Rémi Dulermo, François Brunel, Thierry Dulermo, Rodrigo Ledesma-Amaro, Jérémy Vion, Marion Trassaert, Stéphane Thomas, Jean-Marc Nicaud, Christophe Leplat
BACKGROUND: The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2...
February 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28212391/an-empirical-examination-of-the-factor-structure-of-compassion
#3
Jenny Gu, Kate Cavanagh, Ruth Baer, Clara Strauss
Compassion has long been regarded as a core part of our humanity by contemplative traditions, and in recent years, it has received growing research interest. Following a recent review of existing conceptualisations, compassion has been defined as consisting of the following five elements: 1) recognising suffering, 2) understanding the universality of suffering in human experience, 3) feeling moved by the person suffering and emotionally connecting with their distress, 4) tolerating uncomfortable feelings aroused (e...
2017: PloS One
https://www.readbyqxmd.com/read/28209520/deconstructing-empathy-neuroanatomical-dissociations-between-affect-sharing-and-prosocial-motivation-using-a-patient-lesion-model
#4
Suzanne M Shdo, Kamalini G Ranasinghe, Kelly A Gola, Clinton J Mielke, Paul V Sukhanov, Bruce L Miller, Katherine P Rankin
Affect sharing and prosocial motivation are integral parts of empathy that are conceptually and mechanistically distinct. We used a neurodegenerative disease (NDG) lesion model to more directly examine the neural correlates of these two aspects of real-world empathic responding. The study enrolled 275 participants, including 44 healthy older controls and 231 patients diagnosed with one of five neurodegenerative diseases (75 Alzheimer's disease, 58 behavioral variant frontotemporal dementia (bvFTD), 42 semantic variant primary progressive aphasia (svPPA), 28 progressive supranuclear palsy, and 28 non-fluent variant (nfvPPA)...
February 13, 2017: Neuropsychologia
https://www.readbyqxmd.com/read/28205217/using-cluster-analysis-and-icp-ms-to-identify-groups-of-ecstasy-tablets-in-sao-paulo-state-brazil
#5
Camila Maione, Vanessa Cristina de Oliveira Souza, Loraine Rezende Togni, José Luiz da Costa, Andres Dobal Campiglia, Fernando Barbosa, Rommel Melgaço Barbosa
The variations found in the elemental composition in ecstasy samples result in spectral profiles with useful information for data analysis, and cluster analysis of these profiles can help uncover different categories of the drug. We provide a cluster analysis of ecstasy tablets based on their elemental composition. Twenty-five elements were determined by ICP-MS in tablets apprehended by Sao Paulo's State Police, Brazil. We employ the K-means clustering algorithm along with C4.5 decision tree to help us interpret the clustering results...
February 15, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28199040/isotopic-half-life-and-enrichment-factor-in-two-species-of-european-freshwater-fish-larvae-an-experimental-approach
#6
Adrien Latli, Nicolas Sturaro, Nelson Desjardin, Loïc N Michel, William Otjacques, Gilles Lepoint, Patrick Kestemont
RATIONALE: Stable isotope ratios of carbon and nitrogen are valuable tools for field ecologists to use to analyse animal diets. However, the application of these tools requires knowledge of the tissue enrichment factor (TEF) and half-life (HL). We experimentally compared TEF and HL in 2 freshwater fish larvae. We hypothesised that chub had a better growth/tissue replacement ratio than roach, due to the use of a food closer to their natural diet. METHODS: We determined the isotopic HL, the TEF and the contribution of growth or metabolic tissue replacement to dynamic isotopic incorporation...
February 15, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28198682/molecular-epidemiology-and-antimicrobial-resistance-phenotypes-of-acinetobacter-baumannii-isolated-from-patients-in-three-hospitals-in-southern-vietnam
#7
Nguyen Tuan Anh, Tran Vu Thieu Nga, Huynh Minh Tuan, Nguyen Si Tuan, Dao Minh Y, Nguyen Van Vinh Chau, Stephen Baker, Ho Huynh Thuy Duong
Multidrug resistance in the nosocomial pathogen Acinetobacter baumannii limits therapeutic options and impacts on clinical care. Resistance against carbapenems, a group of last-resort antimicrobials for treating multidrug-resistant (MDR) A. baumannii infections, is associated with the expression (and over-expression) of carbapenemases encoded by the blaOXA genes. The aim of this study was to determine the prevalence of antimicrobial-resistant A. baumannii associated with infection in three hospitals in southern Vietnam and to characterize the genetic determinants associated with resistance against carbapenems...
January 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28197724/histological-and-immunohistochemical-characteristics-of-undifferentiated-small-round-cell-sarcomas-associated-with-cic-dux4-and-bcor-ccnb3-fusion-genes
#8
Yuichi Yamada, Masaaki Kuda, Kenichi Kohashi, Hidetaka Yamamoto, Junkichi Takemoto, Takeaki Ishii, Kunio Iura, Akira Maekawa, Hirofumi Bekki, Takamichi Ito, Hiroshi Otsuka, Makoto Kuroda, Yumi Honda, Shinji Sumiyoshi, Takeshi Inoue, Naoe Kinoshita, Atsushi Nishida, Kyoko Yamashita, Ichiro Ito, Shizuo Komune, Tomoaki Taguchi, Yukihide Iwamoto, Yoshinao Oda
CIC-DUX4 and BCOR-CCNB3 fusion-gene-associated small round cell sarcomas account for a proportion of pediatric small round cell sarcomas, but their pathological features have not been sufficiently clarified. We reviewed a large number of soft tissue tumors registered at our institution, retrieved the cases of unclassified tumors with a small round cell component, and subjected them to histopathological, immunohistochemical, and gene profile analysis. We reviewed 164 cases of unclassified tumors with a small round cell component and analyzed them by RT-PCR and FISH...
February 14, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28197720/single-nucleotide-polymorphisms-in-the-whole-genome-sequence-data-of-shiga-toxin-producing-escherichia-coli-o157-h7-h-strains-by-cultivation
#9
Eiji Yokoyama, Shinichiro Hirai, Taichiro Ishige, Satoshi Murakami
Nine Shiga toxin-producing Escherichia coli O157:H7/H- (O157) strains were serially cultured three times on LB agar plates. After each sub-culture, five colonies were picked for DNA isolation and whole genome sequence (WGS) analysis. After exclusion of possible recombination-related SNPs, 11, 9, and 34 single-nucleotide polymorphisms (SNPs) were detected in genes in the backbone, O-island, and mobile elements gene categories. This suggested that those SNPs due to cultivation could influence the threshold value set for molecular epidemiological studies of O157...
February 14, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28196413/alu-derived-alternative-splicing-events-specific-to-macaca-lineages-in-ctsf-gene
#10
Ja-Rang Lee, Sang-Je Park, Young-Hyun Kim, Se-Hee Choe, Hyeon-Mu Cho, Sang-Rae Lee, Sun-Uk Kim, Ji-Su Kim, Bo-Woong Sim, Bong-Seok Song, Kang-Jin Jeong, Youngjeon Lee, Yeung Bae Jin, Philyong Kang, Jae-Won Huh, Kyu-Tae Chang
Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues...
February 14, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28195027/the-anthropo-scene-a-guide-for-the-perplexed
#11
Jamie Lorimer
The scientific proposal that the Earth has entered a new epoch as a result of human activities - the Anthropocene - has catalysed a flurry of intellectual activity. I introduce and review the rich, inchoate and multi-disciplinary diversity of this Anthropo-scene. I identify five ways in which the concept of the Anthropocene has been mobilized: scientific question, intellectual zeitgeist, ideological provocation, new ontologies and science fiction. This typology offers an analytical framework for parsing this diversity, for understanding the interactions between different ways of thinking in the Anthropo-scene, and thus for comprehending elements of its particular and peculiar sociabilities...
February 2017: Social Studies of Science
https://www.readbyqxmd.com/read/28194995/aerobic-anoxygenic-phototrophs-in-gold-mine-tailings-in-nopiming-provincial-park-manitoba-canada
#12
Elizabeth Hughes, Breanne Head, Chris Maltman, Michele Piercey-Normore, Vladimir Yurkov
A sampling trip to Central Gold Mine, Nopiming Provincial Park, Canada, was taken in September 2011. Abundance, distribution, and physiology of aerobic anoxygenic phototrophs (AAP) from 4 locations were studied. Enumeration revealed 14.6% of culturable microbes were AAP. Five strains (NM4.16, NM4.18, C4, C9, C11) were chosen for analysis. All grow best on complex media without vitamin requirements and with an optimal pH 7.0-8.0, with strain C4 preferring pH 6.0. Strain NM4.18 tolerates the highest pH 11.0. Optimal temperature for all is 28 °C (range of 2-37 °C except NM4...
February 14, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28192796/analysis-of-the-prpf31-gene-in-spanish-autosomal-dominant-retinitis-pigmentosa-patients-a-novel-genomic-rearrangement
#13
Inmaculada Martin-Merida, Rocio Sanchez-Alcudia, Patricia Fernandez-San Jose, Fiona Blanco-Kelly, Raquel Perez-Carro, Luciana Rodriguez-Jacy da Silva, Berta Almoguera, Blanca Garcia-Sandoval, Maria Isabel Lopez-Molina, Almudena Avila-Fernandez, Miguel Carballo, Marta Corton, Carmen Ayuso
Purpose: The aim was to determine the prevalence of PRPF31 mutations in a cohort of Spanish autosomal dominant retinitis pigmentosa (adRP) families to deepen knowledge of the pathogenic mechanisms underlying the disease and to assess genotype-phenotype correlations. Methods: A cohort of 211 adRP patients was screened for variants in PRPF31 by using a combined strategy comprising next-generation sequencing approaches and copy-number variation (CNV) analysis. Quantitative RT-PCR and CNV analysis of the regulatory MSR1 element were also performed to assess PRPF31 gene expression...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28191615/trace-element-measurement-for-assessment-of-dog-food-safety
#14
Elisabete A De Nadai Fernandes, Camila Elias, Márcio Arruda Bacchi, Peter Bode
The quality of dog diets depends on adequate ingredients capable of providing optimal nutrition and free of contaminants, for promoting long-term health. Trace elements in 95 samples of dry food for dog puppies (n = 32) and adults (n = 63) of various brands were measured using instrumental neutron activation analysis (INAA). The mass fractions of most elements were within the permissible limits for dogs. Aluminum, antimony, and uranium presented fairly high levels in some samples, which may imply health risks...
February 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28187319/quantitative-diagnosis-of-breast-tumors-by-characterization-of-viscoelastic-behavior-of-healthy-breast-tissue
#15
N Madani, A Mojra
The main objective of the present study is to propose a model for characterizing the viscoelastic behavior of the healthy breast tissue. The study population consisted of 60 healthy regions of the breast tissue belonged to 9 female cases. To accomplish this, the attending cases were examined by a robot-assisted device and the mechanical stress resulted from an applied compressive strain was measured. Correlation between the experimental stress and the strain data identifies the breast tissue mechanical behavior...
February 3, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28186489/folding-behavior-of-a-t-shaped-ribosome-binding-translation-enhancer-implicated-in-a-wide-spread-conformational-switch
#16
My-Tra Le, Wojciech K Kasprzak, Taejin Kim, Feng Gao, Megan Yl Young, Xuefeng Yuan, Bruce A Shapiro, Joonil Seog, Anne E Simon
Turnip crinkle virus contains a T-shaped, ribosome-binding, translation enhancer (TSS) in its 3'UTR that serves as a hub for interactions throughout the region. The viral RNA-dependent RNA polymerase (RdRp) causes the TSS/surrounding region to undergo a conformational shift postulated to inhibit translation. Using optical tweezers (OT) and steered molecular dynamic simulations (SMD), we found that the unusual stability of pseudoknotted element H4a/Ψ3 required five upstream adenylates, and H4a/Ψ3 was necessary for cooperative association of two other hairpins (H5/H4b) in Mg(2+)...
February 10, 2017: ELife
https://www.readbyqxmd.com/read/28183596/a-numerical-study-on-the-application-of-the-functionally-graded-materials-in-the-stent-design
#17
Arezoo Khosravi, Hossein Bahreinizad, Milad Salimi Bani, Alireza Karimi
Undesirable deformation of the stent can induce a significant amount of injure not only to the blood vessel but also to the plaque. The objective of this study was to reduce/minimize these undesirable deformations by the application of Functionally Graded Materials (FGM). To do this, Finite Element (FE) method was employed to simulate the expansion of a stent and the corresponding displacement of the stenosis plaque. Three hyperelastic plaque types as well as five elastoplastic stents were simulated. Dogboning, foreshortening, maximum stress in the plaque, and the pressure which is needed to fully expand the stent for different stent materials, were acquired...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28183146/prenatal-and-postnatal-management-of-gastroschisis-in-german-speaking-countries-is-there-a-standardized-management
#18
Katharina Schib, Marc Schumacher, Martin Meuli, Sasha Tharakan, Ulrike Subotic
Introduction Evidence-based guidelines or protocols regarding the perinatal management of babies born with gastroschisis are lacking. The aim of this work is to evaluate the different current treatment modalities for newborns with gastroschisis during the perinatal period in the German-speaking countries Germany, Austria, and Switzerland. These data could serve as a starting point for the development of a multicenter randomized controlled trial. Materials and Methods A questionnaire was developed with 30 questions divided into five sections: (1) prenatal diagnosis, (2) fetal therapy, (3) mode and timing of delivery, (4) operative management, and (5) postoperative management...
February 9, 2017: European Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28182581/application-of-single-and-dual-energy-ct-brain-tissue-segmentation-to-pet-monitoring-of-proton-therapy
#19
Bianca Berndt, Guillaume Landry, Florian Schwarz, Thomas Tessonnier, Florian Kamp, Georgios Dedes, Christian Thieke, Matthias Wuerl, Christopher Kurz, Ute Ganswindt, Frank Verhaegen, Juergen Debus, Claus Belka, Wieland Sommer, Maximilian Reiser, Julia Bauer, Katia Parodi
The purpose of this work was to evaluate the ability of single and dual energy computed tomography (SECT, DECT) to estimate tissue composition and density for usage in Monte Carlo (MC) simulations of irradiation induced β<sup>+</sup> activity distributions. This was done to assess the impact on positron emission tomography (PET) range verification in proton therapy. A DECT-based brain tissue segmentation method was developed for white matter (WM), grey matter (GM) and cerebrospinal fluid (CSF)...
February 9, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28182069/role-of-morphometry-in-the-cytological-differentiation-of-benign-and-malignant-thyroid-lesions
#20
Pallavi Khatri, Monisha Choudhury, Manjula Jain, Shaji Thomas
CONTEXT: Thyroid nodules represent a common problem, with an estimated prevalence of 4-7%. Although fine needle aspiration cytology (FNAC) has been accepted as a first line diagnostic test, the rate of false negative reports of malignancy is still high. Nuclear morphometry is the measurement of nuclear parameters by image analysis. Image analysis can merge the advantages of morphologic interpretation with those of quantitative data. AIMS: To evaluate the nuclear morphometric parameters in fine needle aspirates of thyroid lesions and to study its role in differentiating benign from malignant thyroid lesions...
January 2017: Journal of Cytology
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