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https://www.readbyqxmd.com/read/27906459/integrative-genomic-analysis-identifies-ancestry-related-expression-quantitative-trait-loci-on-dna-polymerase-%C3%AE-and-supports-the-association-of-genetic-ancestry-with-survival-disparities-in-head-and-neck-squamous-cell-carcinoma
#1
Meganathan P Ramakodi, Karthik Devarajan, Elizabeth Blackman, Denise Gibbs, Danièle Luce, Jacqueline Deloumeaux, Suzy Duflo, Jeffrey C Liu, Ranee Mehra, Rob J Kulathinal, Camille C Ragin
BACKGROUND: African Americans with head and neck squamous cell carcinoma (HNSCC) have a lower survival rate than whites. This study investigated the functional importance of ancestry-informative single-nucleotide polymorphisms (SNPs) in HNSCC and also examined the effect of functionally important genetic elements on racial disparities in HNSCC survival. METHODS: Ancestry-informative SNPs, RNA sequencing, methylation, and copy number variation data for 316 oral cavity and laryngeal cancer patients were analyzed across 178 DNA repair genes...
December 1, 2016: Cancer
https://www.readbyqxmd.com/read/27906260/parameterization-of-the-mueller-matrix
#2
Colin J R Sheppard
A deterministic Mueller matrix (Mueller-Jones matrix) contains seven independent parameters. By writing the so-called coherence vector in parametric form, the Mueller matrix can also be written in parametric form, where the matrix elements automatically satisfy the known relationships between each other. Three of these parameters are also related to the so-called anisotropy coefficients. The approach is generalized to express all 16 elements of a general Mueller matrix in terms of a scalar and five three-dimensional vectors...
December 1, 2016: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/27906041/implementation-of-the-better-2-program-a-qualitative-study-exploring-barriers-and-facilitators-of-a-novel-way-to-improve-chronic-disease-prevention-and-screening-in-primary-care
#3
Nicolette Sopcak, Carolina Aguilar, Mary Ann O'Brien, Candace Nykiforuk, Kris Aubrey-Bassler, Richard Cullen, Eva Grunfeld, Donna Patricia Manca
BACKGROUND: BETTER (Building on Existing Tools to Improve Chronic Disease Prevention and Screening in Primary Care) is a patient-based intervention to improve chronic disease prevention and screening (CDPS) for cardiovascular disease, diabetes, cancer, and associated lifestyle factors in patients aged 40 to 65. The key component of BETTER is a prevention practitioner (PP), a health care professional with specialized skills in CDPS who meets with patients to develop a personalized prevention prescription, using the BETTER toolkit and Brief Action Planning...
December 1, 2016: Implementation Science: IS
https://www.readbyqxmd.com/read/27905177/expressions-of-cultural-safety-in-public-health-nursing-practice
#4
Anna Richardson, Judy Yarwood, Sandra Richardson
Cultural safety is an essential concept within New Zealand nursing that is formally linked to registration and competency-based practice certification. Despite its centrality to New Zealand nursing philosophies and the stated expectation of cultural safety as a practice element, there is limited evidence of its application in the literature. This research presents insight into public health nurse's (PHN) experiences, demonstrating the integration of cultural safety principles into practice. These findings emerged following secondary analysis of data from a collaborative, educative research project where PHNs explored the use of family assessment tools...
November 30, 2016: Nursing Inquiry
https://www.readbyqxmd.com/read/27903268/long-term-outdoor-air-pollution-and-dna-methylation-in-circulating-monocytes-results-from-the-multi-ethnic-study-of-atherosclerosis-mesa
#5
Gloria C Chi, Yongmei Liu, James W MacDonald, R Graham Barr, Kathleen M Donohue, Mark D Hensley, Lifang Hou, Charles E McCall, Lindsay M Reynolds, David S Siscovick, Joel D Kaufman
BACKGROUND: DNA methylation may mediate effects of air pollution on cardiovascular disease. The association between long-term air pollution exposure and DNA methylation in monocytes, which are central to atherosclerosis, has not been studied. We investigated the association between long-term ambient air pollution exposure and DNA methylation (candidate sites and global) in monocytes of adults (aged ≥55). METHODS: One-year average ambient fine particulate matter (PM2...
December 1, 2016: Environmental Health: a Global Access Science Source
https://www.readbyqxmd.com/read/27900689/novel-tools-for-the-functional-expression-of-metagenomic-dna
#6
Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, Karl-Erich Jaeger
Functional expression of genes from metagenomic libraries is limited by various factors including inefficient transcription and/or translation of target genes as well as improper folding and assembly of the corresponding proteins caused by the lack of appropriate chaperones and cofactors. It is now well accepted that the use of different expression hosts of distinct phylogeny and physiology can dramatically increase the rate of success. In the following chapter, we therefore describe tools and protocols allowing for the comparative heterologous expression of genes in five bacterial expression hosts, namely Escherichia coli, Pseudomonas putida, Bacillus subtilis, Burkholderia glumae, and Rhodobacter capsulatus...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27899601/disprot-7-0-a-major-update-of-the-database-of-disordered-proteins
#7
Damiano Piovesan, Francesco Tabaro, Ivan Mičetić, Marco Necci, Federica Quaglia, Christopher J Oldfield, Maria Cristina Aspromonte, Norman E Davey, Radoslav Davidović, Zsuzsanna Dosztányi, Arne Elofsson, Alessandra Gasparini, András Hatos, Andrey V Kajava, Lajos Kalmar, Emanuela Leonardi, Tamas Lazar, Sandra Macedo-Ribeiro, Mauricio Macossay-Castillo, Attila Meszaros, Giovanni Minervini, Nikoletta Murvai, Jordi Pujols, Daniel B Roche, Edoardo Salladini, Eva Schad, Antoine Schramm, Beata Szabo, Agnes Tantos, Fiorella Tonello, Konstantinos D Tsirigos, Nevena Veljković, Salvador Ventura, Wim Vranken, Per Warholm, Vladimir N Uversky, A Keith Dunker, Sonia Longhi, Peter Tompa, Silvio C E Tosatto
The Database of Protein Disorder (DisProt, URL: www.disprot.org) has been significantly updated and upgraded since its last major renewal in 2007. The current release holds information on more than 800 entries of IDPs/IDRs, i.e. intrinsically disordered proteins or regions that exist and function without a well-defined three-dimensional structure. We have re-curated previous entries to purge DisProt from conflicting cases, and also upgraded the functional classification scheme to reflect continuous advance in the field in the past 10 years or so...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899073/recommendations-for-the-analysis-of-individually-randomised-controlled-trials-with-clustering-in-one-arm-a-case-of-continuous-outcomes
#8
Laura Flight, Annabel Allison, Munyaradzi Dimairo, Ellen Lee, Laura Mandefield, Stephen J Walters
BACKGROUND: In an individually randomised controlled trial where the treatment is delivered by a health professional it seems likely that the effectiveness of the treatment, independent of any treatment effect, could depend on the skill, training or even enthusiasm of the health professional delivering it. This may then lead to a potential clustering of the outcomes for patients treated by the same health professional, but similar clustering may not occur in the control arm. Using four case studies, we aim to provide practical guidance and recommendations for the analysis of trials with some element of clustering in one arm...
November 29, 2016: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/27898817/genome-wide-identification-of-the-ap2-erf-gene-family-involved-in-active-constituent-biosynthesis-in
#9
A J Ji, H M Luo, Z C Xu, X Zhang, Y J Zhu, B S Liao, H Yao, J Y Song, S L Chen
Tanshinones and phenolic acids are the major bioactive constituents in the traditional medicinal crop ; however, transcription factors (TFs) are seldom investigated with regard to their regulation of the biosynthesis of these compounds. Here a complete overview of the APETALA2/ethylene-responsive factor (AP2/ERF) transcription factor family in is provided, including phylogeny, gene structure, conserved motifs, and gene expression profiles of different organs (root, stem, leaf, flower) and root tissues (periderm, phloem, xylem)...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898372/gamma-radiation-induced-thermoluminescence-trace-element-and-paramagnetic-defect-of-quartz-from-the-sambagawa-metamorphic-belt-central-shikoku-japan
#10
T Chuenpee, O Nishikawa, Y Kon, K Ninagawa, S Toyoda, T Ogata, T Uchida, I Takashima
This study analyzes the Thermoluminescence (TL) emissions for five emission bands, trace element concentrations and defects in quartz grains extracted from metamorphic rocks and quartz veins in the Sambagawa metamorphic belt, central Shikoku. An emission of 500nm with 195, 245, and 320-325°C glow peaks are observed through the lowest to highest grade samples. A 450nm emission band with intense 195 and 245°C glow peaks and a 320-325°C shoulder peak is found in the higher grade samples. A 570nm emission band with a 170°C glow peak is observed in the samples derived from the lower grade zones...
November 17, 2016: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27896914/an-innovative-textbook-design-and-implementation
#11
Rafael Ortega, Vafa Akhtar-Khavari, Paul Barash, Sam Sharar, M Christine Stock
BACKGROUND: The 'flipped classroom' instructional strategy has gained much momentum in educational discourse, yet no single educational offering exists in postgraduate medical education (PME) to systematically replace the lecture element of didactic sessions. This article describes the design and implementation of Clinical Anesthesia Fundamentals, the first known textbook in PME addressing this gap in resources through the integration of full-length interactive multimedia-enhanced video lectures...
November 29, 2016: Clinical Teacher
https://www.readbyqxmd.com/read/27896678/no-clinically-important-difference-in-knee-scores-or-instability-between-transtibial-and-inlay-techniques-for-pcl-reconstruction-a-systematic-review
#12
REVIEW
Young-Soo Shin, Hyun-Jung Kim, Dae-Hee Lee
BACKGROUND: It is unclear whether the biomechanical superiority of the inlay technique over the transtibial technique, arising from avoidance of the killer turn at the graft-tunnel margin of the proximal tibia during posterior cruciate ligament (PCL) reconstruction, leads to better knee scores or greater knee stability. QUESTIONS/PURPOSES: This systematic review was designed to compare Tegner and Lysholm scores, and posterior residual laxity of the knee, between single-bundle PCL reconstruction using transtibial and inlay techniques...
November 28, 2016: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/27892840/adhesive-cementation-promotes-higher-fatigue-resistance-to-zirconia-crowns
#13
F Campos, L F Valandro, S A Feitosa, C J Kleverlaan, A J Feilzer, N de Jager, M A Bottino
OBJECTIVE: The aim of this study was to investigate the influence of the cementation strategy on the fatigue resistance of zirconia crowns. The null hypothesis was that the cementation strategy would not affect the fatigue resistance of the crowns. METHODS AND MATERIALS: Seventy-five simplified molar tooth crown preparations were machined in glass fiber-filled epoxy resin. Zirconia crowns were designed (thickness=0.7 mm), milled by computer-aided design/computer-aided manufacturing, and sintered, as recommended...
November 28, 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27892525/sxt-r391-integrative-and-conjugative-elements-in-proteus-species-reveal-abundant-genetic-diversity-and-multidrug-resistance
#14
Xinyue Li, Yu Du, Pengcheng Du, Hang Dai, Yujie Fang, Zhenpeng Li, Na Lv, Baoli Zhu, Biao Kan, Duochun Wang
SXT/R391 integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are found in most members of Enterobacteriaceae. Here, we determined fifteen SXT/R391 ICEs carried by Proteus isolates from food (4.2%) and diarrhoea patients (17.3%). BLASTn searches against GenBank showed that the fifteen SXT/R391 ICEs were closely related to that from different Enterobacteriaceae species, including Proteus mirabilis. Using core gene phylogenetic analysis, the fifteen SXT/R391 ICEs were grouped into six distinct clusters, including a dominant cluster and three clusters that have not been previously reported in Proteus isolates...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27889456/synthesis-of-new-morpholine-connected-pyrazolidine-derivatives-and-their-antimicrobial-antioxidant-and-cytotoxic-activities
#15
Radhakrishnan Surendra Kumar, Meera Moydeen, Salem S Al-Deyab, Aseer Manilal, Akbar Idhayadhulla
A simple and convenient one-pot four-component synthesis of morpholine-connected pyrazolidine derivatives 2a-f and 4a-f was developed using direct metal-free catalysis, with the identities of the synthesized compounds confirmed by IR, NMR ((1)H and (13)C), mass spectrometry, and elemental analysis. The prepared compounds were inspected for antimicrobial, antioxidant, and cytotoxic activities. Antimicrobial and antifungal activities against five bacterial and four fungal pathogens, respectively, were investigated using the disc diffusion technique...
November 14, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27889339/preparation-and-copper-ions-adsorption-properties-of-thiosemicarbazide-chitosan-from-squid-pens
#16
Yue-Cheng Lin, Hong-Peng Wang, Faryal Gohar, Muhammad Haseeb Ullah, Xiang Zhang, Dong-Fang Xie, Hui Fang, Jun Huang, Jun-Xing Yang
Chitosan was prepared by alkaline N-deacetylation of β-chitin from squid pens. Thiosemicarbazide group was introduced to chitosan via formaldehyde-derived linkages, and thiosemicarbazide chitosan (TSFCS) with different degrees of substitution (DS) was synthesized. The DS values of TSFCS calculated by elemental analysis were 0.19, 0.36 and 0.63. The structure of the TSFCS was confirmed by elemental analysis, FTIR, XRD, TGA and SEM. The adsorption capacity of Cu(II) ions by TSFCS showed good correlation with the DS and pH (pH range 2...
November 24, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27887829/use-of-simulated-epithelial-lung-fluid-in-assessing-the-human-health-risk-of-pb-in-urban-street-dust
#17
John R Dean, Nwabueze I Elom, Jane A Entwistle
In many urban contexts, non-dietary Pb exposure from street dusts may add to the overall exposure burden, and the presence of high total Pb content is well documented in urban street dust from across the globe. Given the increasing recognition of the potential adverse health effects from both the quantity and the chemical and physical composition of the inhaled fraction, and the recognition that it is the soluble fraction rather than the total element content that has more direct links to health effects, attention has focused in this study on the human health risks via this exposure pathway...
November 22, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27886991/radiation-dose-for-pediatric-and-young-adult-ct-a-survey-to-establish-age-based-reference-levels-of-2015-2016-in-korea
#18
MoonChan Kim, KwangHyun Chang, JeongHoon Hwang, YoonChul Nam, DongKyoon Han, Joon Yoon
To assess the doses delivered to pediatric patients during computed tomography (CT) examinations of the brain, chest, high-resolution lung and abdomen, and to establish diagnostic reference levels (DRLs) for various age groups in Korea. Dose survey was done to the 19 hospitals performing CT on children, addressing the scan parameters, volume CT dose index (CTDIvol) and dose length product (DLP). Per five age group (0, 1, 2-5, 6-10, 11-17 y of age), the proposed DRLs for brain, chest, high-resolution lung and abdomen CT are, respectively, in terms of CTDIvol: 18, 23, 26, 31, 36 mGy; 2, 3, 4, 6, 8 mGy; 2, 3, 4, 5, 7 mGy; 3, 4, 5, 6, 9 mGy; and in terms of DLP: 260, 350, 420, 500, 620 mGy•cm; 50, 80, 100, 170, 340 mGy•cm; 30, 40, 60, 90, 280 mGy•cm; 70, 80, 200, 300, 500 mGy•cm...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27881798/acclimation-of-leaf-water-status-and-stem-hydraulics-to-drought-and-tree-neighbourhood-alternative-strategies-among-the-saplings-of-five-temperate-deciduous-tree-species
#19
Torben Lübbe, Bernhard Schuldt, Christoph Leuschner
Adjustment in leaf water status parameters and modification in xylem structure and functioning can be important elements of a tree's response to continued water limitation. In a growth trial with saplings of five co-occurring temperate broad-leaved tree species (genera Fraxinus, Acer, Carpinus, Tilia and Fagus) conducted in moist or dry soil, we compared the drought acclimation in several leaf water status and stem hydraulic parameters. Considering the extremes in the species responses, Fraxinus excelsior L...
November 22, 2016: Tree Physiology
https://www.readbyqxmd.com/read/27881319/influence-of-remaining-tooth-structure-and-restorative-material-type-on-stress-distribution-in-endodontically-treated-maxillary-premolars-a-finite-element-analysis
#20
Junxin Zhu, Qiguo Rong, Xiaoyan Wang, Xuejun Gao
STATEMENT OF PROBLEM: How tooth preparation and material type affect the stress distribution of endodontically treated teeth restored with endocrowns remains unclear. PURPOSE: The purpose of this finite element (FE) study was to determine the influence of the quantity of remaining dental tissues and material type on stress distribution in endodontically treated maxillary premolars using 3-dimensional FE analysis. MATERIAL AND METHODS: Five 3-dimensional FE models were constructed on the basis of the restorative methods used and the quantity of preserved tooth tissues: a sound maxillary premolar, an endodontically treated maxillary premolar restored with composite resin, and endodontically treated maxillary premolars restored with endocrowns with thicknesses of 1...
October 27, 2016: Journal of Prosthetic Dentistry
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