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https://www.readbyqxmd.com/read/28745647/utilization-of-capsules-for-negative-staining-of-viral-samples-within-biocontainment
#1
Candace D Blancett, Mitchell K Monninger, Chrystal A Nguessan, Kathleen A Kuehl, Cynthia A Rossi, Scott P Olschner, Priscilla L Williams, Steven L Goodman, Mei G Sun
Transmission electron microscopy (TEM) is used to observe the ultrastructure of viruses and other microbial pathogens with nanometer resolution. Most biological materials do not contain dense elements capable of scattering electrons to create an image; therefore, a negative stain, which places dense heavy metal salts around the sample, is required. In order to visualize viruses in suspension under the TEM they must be applied to small grids coated with a transparent surface only nanometers thick. Due to their small size and fragility, these grids are difficult to handle and easily moved by air currents...
July 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28743081/mycobacterium-tuberculosis-ppe44-rv2770c-is-involved-in-response-to-multiple-stresses-and-promotes-the-macrophage-expression-of-il-12-p40-and-il-6-via-the-p38-erk-and-nf-%C3%AE%C2%BAb-signaling-axis
#2
Zhaoxiao Yu, Chenhui Zhang, Mingliang Zhou, Qiming Li, Hui Li, Wei Duan, Xue Li, Yonghong Feng, Jianping Xie
Tuberculosis (TB), caused by Mycobacterium tuberculosis, remains a formidable threat to global public health. The successful intracellular persistence of M. tuberculosis significantly contributes to the intractability of tuberculosis. Proline-glutamic acid (PE) and proline-proline-glutamic acid (PPE) are mycobacterial exclusive protein families that widely reported to be involved in the bacterial virulence, physiology and interaction with host. Rv2770c (PPE44), a predicted virulence factor, was up-regulated upon the infected guinea pig lungs...
July 22, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28742838/identification-of-novel-antigen-candidates-for-a-tuberculosis-vaccine-in-the-adult-zebrafish-danio-rerio
#3
Henna Myllymäki, Mirja Niskanen, Kaisa Ester Oksanen, Eleanor Sherwood, Maarit Ahava, Mataleena Parikka, Mika Rämet
Tuberculosis (TB) remains a major global health challenge and the development of a better vaccine takes center stage in fighting the disease. For this purpose, animal models that are capable of replicating the course of the disease and are suitable for the early-stage screening of vaccine candidates are needed. A Mycobacterium marinum infection in adult zebrafish resembles human TB. Here, we present a pre-clinical screen for a DNA-based tuberculosis vaccine in the adult zebrafish using an M. marinum infection model...
2017: PloS One
https://www.readbyqxmd.com/read/28734490/preconception-peer-educators-exploratory-outreach-to-the-bahamas-a-foundation-for-an-international-service-learning-initiative
#4
Gail H Wade, Mary B DeLashmutt, Anne DeCaire, Emily Boyce
A service learning outreach was undertaken to assess the feasibility of adapting the Preconception Peer Educator (PPE) program to Bahamian youth. By focusing on preconception health, the PPE program is a logical step toward ensuring age sensitive and developmentally appropriate education to improve potential birth outcomes and decrease infant mortality rates (IMR) associated with the complex societal problems on Grand Bahama. Concerned with the prevalence of adolescent pregnancy, lack of social support, depression, and failure to complete high school, a nurse midwife invited PPEs from a School of Nursing to meet with stakeholders to introduce the PPE program to Bahamian youth...
July 2017: Journal of Professional Nursing: Official Journal of the American Association of Colleges of Nursing
https://www.readbyqxmd.com/read/28732742/what-do-visitors-know-and-how-do-they-feel-about-contact-precautions
#5
Grace Seibert, Tola Ewers, Anna K Barker, Adam Slavick, Marc-Oliver Wright, Linda Stevens, Nasia Safdar
Patients with Clostridium difficile infection (CDI) are placed in contact precautions. We surveyed 31 visitors of CDI patients to understand their compliance, knowledge, and perceptions of contact precautions. Although most visitors knew where to find the required personal protective equipment, only 42% were fully compliant with gown and gloves. Family members accounted for 90% of visitors, and roughly half of the reasons given for not gowning were related to a lack of perceived risk for family members. Nursing staff are fundamental sources of personal protective equipment (PPE) information for visitors; however, we found variation in staff communication regarding need for visitor PPE use...
July 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28732589/accuracy-of-diagnostic-imaging-modalities-for-classifying-pediatric-eyes-as-papilledema-versus-pseudopapilledema
#6
Melinda Y Chang, Federico G Velez, Joseph L Demer, Laura Bonelli, Peter A Quiros, Anthony C Arnold, Alfredo A Sadun, Stacy L Pineles
PURPOSE: To identify the most accurate diagnostic imaging modality for classifying pediatric eyes as papilledema (PE) or pseudopapilledema (PPE). DESIGN: Prospective observational study. SUBJECTS: Nineteen children between the ages of 5 and 18 years were recruited. Five children (10 eyes) with PE, 11 children (19 eyes) with PPE owing to suspected buried optic disc drusen (ODD), and 3 children (6 eyes) with PPE owing to superficial ODD were included...
July 18, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28723851/indocyanine-green-angiography-of-pachychoroid-pigment-epitheliopathy
#7
M Giray Ersoz, Serra Arf, Mumin Hocaoglu, Isil Sayman Muslubas, Murat Karacorlu
PURPOSE: To compare choroidal hyperpermeability and vessel density between eyes with central serous chorioretinopathy (CSC), those with pachychoroid pigment epitheliopathy (PPE) and those with uncomplicated pachychoroid (UCP). METHODS: We retrospectively reviewed indocyanine green angiography images of 60 patients with unilateral active CSC who had PPE (36 eyes) or UCP (24 eyes) in their fellow eyes. We used color thresholding of indocyanine green angiography images to determine choroidal vessel density...
July 18, 2017: Retina
https://www.readbyqxmd.com/read/28708703/preparticipation-sports-physicals-a-comparison-of-single-provider-and-station-based-models
#8
David Lick, Karim Abdel-Aty, Daniel Diaz, Jaspaul Dulku, Sandeep Lochub, Kamran Mir, Robert Ricketts
BACKGROUND: Preparticipation physical examinations (PPEs) are required for children and adolescents before sports participation to identify at-risk athletes. These evaluations can be completed in a traditional office-based setting or in a station-based format. It is unclear if one format is superior to the other in identifying at-risk athletes. METHODS: We performed a retrospective chart review of children and adolescents between the ages 10 and 18 years who received their PPE in our office or in a station-based setting in a local high school between the years 2009 and 2015...
July 11, 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/28704475/protective-effect-of-galectin-9-in-murine-model-of-lung-emphysema-involvement-of-neutrophil-migration-and-mmp-9-production
#9
Yuko Horio, Hidenori Ichiyasu, Keisuke Kojima, Naoki Saita, Yohei Migiyama, Toyohisa Iriki, Kazuhiko Fujii, Toshiro Niki, Mitsuomi Hirashima, Hirotsugu Kohrogi
PURPOSE: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow obstruction and pulmonary emphysema. Persistent inflammation and remodeling of the lungs and airways result in reduced lung function and a lower quality of life. Galectin (Gal)-9 plays a crucial role as an immune modulator in various diseases. However, its role in the pathogenesis of pulmonary emphysema is unknown. This study investigates whether Gal-9 is involved in pulmonary inflammation and changes in emphysema in a porcine pancreatic elastase (PPE)-induced emphysema model...
2017: PloS One
https://www.readbyqxmd.com/read/28699840/distributed-improvisation-a-systems-perspective-of-improvisation-epics-by-led-outdoor-activity-leaders
#10
Margaret J Trotter, Paul M Salmon, Natassia Goode, Michael G Lenné
Improvisation represents the spontaneous and real-time conception and execution of a novel response to an unanticipated situation. In order to benefit from the positive safety potential of this phenomenon, it is necessary to understand what influences its appropriateness and effectiveness. This study has applied the system-based methodology Impromaps to analysing accounts of improvisation aimed at mitigating adverse safety outcomes. These accounts were obtained from led outdoor activity leaders through critical decision method interviews...
July 12, 2017: Ergonomics
https://www.readbyqxmd.com/read/28681657/fatal-poisoning-by-terbufos-following-occupational-exposure
#11
Yue Liang, Fang Tong, Lin Zhang, Wenhe Li, Weisheng Huang, Yiwu Zhou
CONTEXT: Terbufos (TBF) is a class Ia (extremely hazardous) organophosphate pesticide (OP) and its distribution in industrialized countries has been severely restricted. Thus, acute occupational poisoning is rather uncommon. However, it still occurs in rural areas of some developing countries, where the sale of TBF is not controlled and its use is thus not properly regulated. We report a case of a 43-year-old female farmer who died after applying TBF granules. CASE: The patient died within 3 h after applying 20 bags of 5% TBF granules (900 g per bag)...
July 6, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28679520/prevalence-and-injury-patterns-among-electronic-waste-workers-in-the-informal-sector-in-nigeria
#12
Chimere May Ohajinwa, Peter M van Bodegom, Martina G Vijver, Adesola O Olumide, Oladele Osibanjo, Willie J G M Peijnenburg
BACKGROUND: Despite the large volume of e-waste recycled informally, the prevalence of work-related injuries among e-waste workers is unknown. Therefore, this study assessed the prevalence, patterns and factors associated with occupational injuries among e-waste workers in the informal sector in Nigeria. METHODS: This cross-sectional study adopted a multistage sampling method to select 279 respondents from three cities (Ibadan, Lagos and Aba) in Nigeria. A questionnaire was used to obtain information on sociodemographics, work practices and injury occurrences from the respondents in 2015...
July 5, 2017: Injury Prevention: Journal of the International Society for Child and Adolescent Injury Prevention
https://www.readbyqxmd.com/read/28675243/biotech-innovations-in-the-prevention-of-respiratory-infectious-diseases
#13
Julia Valeria Cento, Silvia Barbaliscia, Carlo Federico Perno
review is the creation of a biocompatible, biogel-based nasal filter enriched with AgNPs. Compared to traditional personal protective equipment (PPE), this type of nasal filter has the advantage of combining the antibacterial and antiviral activity of AgNPs with the common filtration capacity shared by other PPEs. This dual mechanism means that AgNP-enriched nasal filters serve to reduce the infecting microbial-load and protect the lower airways, without interfering with the normal respiratory capacity (airflow-resistance <5%)...
July 4, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28673835/when-should-parapneumonic-pleural-effusions-be-drained-in-children
#14
REVIEW
Gilberto Bueno Fischer, Helena Teresinha Mocelin, Cistiano Feijó Andrade, Edgar E Sarria
Pneumonia is an important health problem in children, and parapneumonic pleural effusion (PPE) is a frequent complication. There is no standard strategy for treating PPE, reflected in the few international guidelines that have been published on the issue. Compared to adults, there is no consensus on the utility of pleural fluid analysis in paediatric PPE. This is because of the lack of good evidence either in favour or against it and the risks of procedural sedation for acquiring pleural fluid for analysis to guide management...
June 1, 2017: Paediatric Respiratory Reviews
https://www.readbyqxmd.com/read/28673681/the-association-between-self-perceived-proficiency-of-personal-protective-equipment-and-objective-performance-an-observational-study-during-a-bioterrorism-simulation-drill
#15
Itay Fogel, Osant David, Chaya H Balik, Arik Eisenkraft, Lion Poles, Omri Shental, Michael Kassirer, Tal Brosh-Nissimov
BACKGROUND: The recent Ebola virus disease outbreak emphasized the potential misuse of personal protective equipment (PPE) by health care workers (HCWs) during such an event. We aimed to compare self-perceived proficiency of PPE use and objective performance, and identify predictors of low compliance and PPE misuse. METHODS: An observational study combined with subjective questionnaires were carried out during a bioterror simulation drill. Forty-two observers evaluated performance under PPE...
June 30, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28664541/placebo-response-and-its-determinants-in-children-with-adhd-across-multiple-observers-and-settings-a-randomized-clinical-trial
#16
Weam Fageera, Alexandru Traicu, Sarojini M Sengupta, Marie-Eve Fortier, Zia Choudhry, Aurélie Labbe, Natalie Grizenko, Ridha Joober
This study aims to quantify placebo response (PR) in children with attention deficit hyperactivity disorder (ADHD) as assessed by parents and teachers and to explore some of its determinants. Five hundred and forty children with ADHD (ages 6-12) were recruited to a randomized, double-blind, placebo-controlled crossover trial with methylphenidate. The main outcome variable was Conners' Global Index (CGI), based on assessment of behaviour by parents (CGI-P) and teacher (CGI-T). PR was calculated as the difference between CGI-P/T scores at baseline and placebo week...
June 30, 2017: International Journal of Methods in Psychiatric Research
https://www.readbyqxmd.com/read/28663587/challenge-of-liquid-stressed-protective-materials-and-environmental-persistence-of-ebola-virus
#17
Aidan M Nikiforuk, Todd A Cutts, Steven S Theriault, Bradley W M Cook
After the largest Ebola virus outbreak in history, experts have attempted to answer how the Zaire ebolavirus species emerged in West Africa and caused chains of human-to-human transmission. The widespread and untimely infection of Health Care Workers (HCW) in the affected countries accelerated spread of the virus within the community. Among the reasons attributed to this trend, it must be considered that HCW were exposed to the virus in their occupational environment. The contribution of environmental conditions to the spread of Ebola in West Africa was examined by investigating the effect of temperature/humidity on the virus's environmental persistence and by modeling if saturation (liquid stress) allows for penetration of Ebola virus through personal protective equipment (PPE)...
June 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28662788/clinical-issues-july-2017
#18
Mary J Ogg, Esther M Johnstone
Operation of fluoroscopy equipment Key words: fluoroscopy, mini C-arm, scope of practice, radiologic device. Patient education after radionuclide implantation Key words: radionuclides, brachytherapy, prostate cancer, radiation safety. Personal protective equipment Key words: clean, decontamination, instruments, PPE, tray assembly. Safe staffing patterns Key words: on-call staffing, schedules, safe staffing levels. Counting instruments when laparoscopy procedures convert to open procedures Key words: counts, instrument, laparoscopy, retained surgical items...
July 2017: AORN Journal
https://www.readbyqxmd.com/read/28662749/personal-protective-equipment-in-animal-research
#19
Jason S Villano, Janet M Follo, Mark G Chappell, Morris T Collins
The occupational health and safety program is an integral component of a comprehensive animal care and use program. It is important to mitigate the risk of exposures of animal care and research personnel to allergens and physical, chemical, radiologic, and biologic hazards during the conduct of various tasks. This need is especially true in infectious disease and biocontainment research. One aspect of the program is the provision of personal protective equipment (PPE). Commercially available PPE should be carefully evaluated based on their material composition and performance according to manufacturer data...
June 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28655451/rationale-for-two-influenza-b-lineages-in-seasonal-vaccines-a-meta-regression-study-on-immunogenicity-and-controlled-field-trials
#20
W E P Beyer, A M Palache, M Boulfich, A D M E Osterhaus
B lineage mismatch prompted introduction of quadri-valent influenza vaccines (QIV) with two influenza B viruses representing distinct antigenic lineages. To explore the impact on antibody induction and vaccine effectiveness predicted from antibody (VEab), we performed a systematic literature search on immunogenicity studies conducted to assess antibody superiority of QIV over trivalent influenza vaccine (TIV). Thirteen relevant articles described 31 trials from 2007 and 2013. Log-transformed GMT trial estimates and their variances were converted to clinical protection rates predicted from antibody (PRab)...
June 24, 2017: Vaccine
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