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https://www.readbyqxmd.com/read/28225343/infection-control-and-tuberculosis-in-health-care-workers-an-assessment-of-28-hospitals-in-south-africa
#1
L M O'Hara, A Yassi, E A Bryce, A Janse van Rensburg, M C Engelbrecht, M Zungu, L E Nophale, J M FitzGerald
SETTING: Twenty-eight public hospitals in the Free State Province, South Africa. OBJECTIVE: To examine the association between tuberculosis (TB) infection control (IC) scores in Free State hospitals and the incidence of TB disease among health care workers (HCWs) in 2012. DESIGN: A cross-sectional survey and mixed-methods analysis of TB IC policies, practices and infrastructure using a comprehensive, 83-item IC audit and observation tool. RESULTS: As the total IC score increased, the probability of TB in an HCW at that hospital decreased...
March 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28223967/secretome-prediction-of-two-m-tuberculosis-clinical-isolates-reveals-their-high-antigenic-density-and-potential-drug-targets
#2
Fernanda Cornejo-Granados, Zyanya L Zatarain-Barrón, Vito A Cantu-Robles, Alfredo Mendoza-Vargas, Camilo Molina-Romero, Filiberto Sánchez, Luis Del Pozo-Yauner, Rogelio Hernández-Pando, Adrián Ochoa-Leyva
The Excreted/Secreted (ES) proteins play important roles during Mycobacterium tuberculosis invasion, virulence, and survival inside the host and they are a major source of immunogenic proteins. However, the molecular complexity of the bacillus cell wall has made difficult the experimental isolation of the total bacterial ES proteins. Here, we reported the genomes of two Beijing genotype M. tuberculosis clinical isolates obtained from patients from Vietnam (isolate 46) and South Africa (isolate 48). We developed a bioinformatics pipeline to predict their secretomes and observed that ~12% of the genome-encoded proteins are ES, being PE, PE-PGRS, and PPE the most abundant protein domains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28220574/selective-use-of-the-electrocardiogram-in-pediatric-preparticipation-athletic-examinations-among-pediatric-primary-care-providers
#3
Bradley C Clark, Joshua M Hayman, Charles I Berul, Kristin M Burns, Jonathan R Kaltman
OBJECTIVES: Recent literature examining insurance administrative data suggests that a selective approach, with concurrent history and physical exam (H&P), for obtaining an electrocardiogram (ECG) as a part of a preparticipation examination (PPE) for pediatric athletes is commonly used in the primary care setting demonstrating a high rate of disease detection. We sought to understand practice patterns of providers with regard to usage of ECG as a part of PPE. METHODS: Utilizing an online survey, we queried primary care providers regarding their practice patterns, rationale, and concerns regarding use of ECGs as a part of the PPE...
February 21, 2017: Annals of Noninvasive Electrocardiology
https://www.readbyqxmd.com/read/28216628/genome-wide-primary-transcriptome-analysis-of-h2-producing-archaeon-thermococcus-onnurineus-na1
#4
Suhyung Cho, Min-Sik Kim, Yujin Jeong, Bo-Rahm Lee, Jung-Hyun Lee, Sung Gyun Kang, Byung-Kwan Cho
In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of the H2-producing archaeon Thermococcus onnurineus NA1. We identified 1,082 purine-rich transcription initiation sites along with well-conserved TATA box, A-rich B recognition element (BRE), and promoter proximal element (PPE) motif in promoter regions, a high pyrimidine nucleotide content (T/C) at the -1 position, and Shine-Dalgarno (SD) motifs (GGDGRD) in 5' untranslated regions (5' UTRs)...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28205097/identifying-and-characterising-ppe7-rv0354c-high-activity-binding-peptides-and-their-role-in-inhibiting-cell-invasion
#5
Diana P Díaz, Marisol Ocampo, Yahson Varela, Hernando Curtidor, Manuel A Patarroyo, Manuel E Patarroyo
This study was aimed at characterising the PPE7 protein from the PE/PPE protein family. The presence and transcription of the rv0354c gene in the Mycobacterium tuberculosis complex was determined and the subcellular localisation of the PPE7 protein on mycobacterial membrane was confirmed by immunoelectron microscope. Two peptides were identified as having high binding activity (HABPs) and were tested in vitro regarding the invasion of Mycobacterium tuberculosis H37Rv. HABP 39224 inhibited invasion in A549 epithelial cells and U937 macrophages by more than 50%, whilst HABP 39225 inhibited invasion by 40% in U937 cells...
February 15, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28202357/variations-in-the-arterial-blood-supply-to-the-penis-and-the-accessory-pudendal-artery-a-meta-analysis-and-review-of-its-implications-in-radical-prostatectomy
#6
Brandon Michael Henry, Przemysław A Pękala, Jens Vikse, Beatrice Sanna, Bendik Skinningsrud, Karolina Saganiak, Jerzy A Walocha, Krzysztof A Tomaszewski
PURPOSE: The aim of this meta-analysis was to analyze the available literature and provide comprehensive data on the prevalence and variations of accessory pudendal artery (APA) and the three types of penile blood supply (Type 1 - internal pudendal artery (IPA) only, Type 2 - IPA and APA), Type 3 - APA only) MATERIALS AND METHODS: We conducted an extensive search of the major databases and identified 23 studies (4,945 patients) suitable for inclusion in this meta-analysis. Studies eligible for inclusion included cadaveric, imaging or intraoperative studies...
February 12, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28196399/relationship-of-the-recurrent-laryngeal-nerve-to-the-inferior-thyroid-artery-a-comparison-of-findings-from-two-systematic-reviews
#7
REVIEW
Anthony P Polednak
Systematic reviews (SRs) of anatomical studies may include a meta-analysis (MA) that provides weighted averages as pooled estimates of prevalence. The relationship of the recurrent laryngeal nerve (RLN) to the inferior thyroid artery (ITA) or its branches has been assessed in two published SRs, one without MA of 32 studies (SR1) and the other with MA of 79 studies (SR2). Both SRs reported differences in RLN-ITA patterns (in three categories) by side of the body, but the anterior pattern was less frequent in SR1 vs...
February 14, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28196091/pre-breeding-of-lentil-lens-culinaris-medik-for-herbicide-resistance-through-seed-mutagenesis
#8
Muhammad Rizwan, Muhammad Aslam, Muhammad Jawad Asghar, Ghulam Abbas, Tariq Mahmud Shah, Hussein Shimelis
Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28...
2017: PloS One
https://www.readbyqxmd.com/read/28179609/physical-and-environmental-hazards-in-the-prosthetics-and-orthotics-workshop-a-pilot-study
#9
Sarah Anderson, Rwth Stuckey, Diana Poole, Jodi Oakman
: Background; Prosthetists and Orthotists (P&O) are exposed to physical hazards within the workshop environment. Concern regarding these exposures has been expressed by P&Os; however, little research has been undertaken. Exposures to noise and volatile organic compounds in amounts larger than statutorily allowed can have adverse short and long term consequences on people's health. OBJECTIVES: To identify and quantify hazardous noise and chemical exposures in a typical P&O workplace...
February 7, 2017: Industrial Health
https://www.readbyqxmd.com/read/28178912/do-medical-students-receive-training-in-correct-use-of-personal-protective-equipment
#10
Amrita John, Myreen E Tomas, Aditya Hari, Brigid M Wilson, Curtis J Donskey
BACKGROUND: Healthcare personnel often use incorrect technique for donning and doffing of personal protective equipment (PPE). OBJECTIVE: We tested the hypothesis that medical students receive insufficient training on correct methods for donning and doffing PPE. METHODS: We conducted a cross-sectional survey of medical students on clinical rotations at two teaching hospitals to determine the type of training they received in PPE technique...
2017: Medical Education Online
https://www.readbyqxmd.com/read/28177373/a-strategy-to-improve-nitrogen-utilization-reduce-environmental-impact-and-increase-performance-and-antioxidant-capacity-of-fattening-lambs-using-pomegranate-peel-extract
#11
M Rajabi, Y Rouzbehan, J Rezaei
Dietary natural plant secondary metabolites (PSM) extracted from a pomegranate peel (PP) byproduct have the potential to improve milk yield and the milk fatty acid profile of dairy cows. This experiment was performed to assess the influence of different dietary levels of PP extract (PPE) on feedlot performance, ruminal status, nutrient utilization, and antioxidant status in fattening Moghani lambs. Thirty-two lambs (initial BW of 22 ± 1.2 kg) were used in a completely randomized design with a 72-d period and 4 treatments: PPE0 (no extract), PPE15 (15 mL PPE/kg of diet DM), PPE30 (30 mL PPE/kg of diet DM), and PPE45 (45 mL PPE/kg of diet DM)...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28166125/symptoms-of-nervous-system-related-disorders-among-workers-exposed-to-occupational-noise-and-vibration-in-korea
#12
Seunghyun Lee, Wanhyung Lee, Jaehoon Roh, Jong-Uk Won, Jin-Ha Yoon
OBJECTIVE: The aim of this study was to determine the relationship between vibration and noise exposure in the workplace and certain nervous system related symptoms (NSRS) among Korean workers. METHODS: Using data from the fourth Korean Working Conditions Survey, we investigated the influence of vibration and noise with three categories; none, mild, and severe, on sleep disturbance, overall fatigue, and headache/eye strain using logistic regression analysis with stratification by personal protective equipment (PPE) wearing status...
February 2017: Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28164581/evaluation-of-pleural-fluid-lactate-for-diagnosis-and-management-of-parapneumonic-pleural-effusion
#13
Jose D Santotoribio, Hiba Alnayef-Hamwie, Paula Batalha-Caetano, Santiago Perez-Ramos, Maria J Sanchez Del Pino
BACKGROUND: The aim was to measure the accuracy of pleural fluid lactate concentration for diagnosis of parapneumonic pleural effusion (PPE) and to discriminate between uncomplicated (UPPE) and complicated PPE (CPPE). METHODS: Pleural fluid lactate was measured in patients with pleural effusion. Patients were classified into two groups according to the etiology of pleural effusion: PPE and NOT PPE. RESULTS: We studied 173 patients. Thirty patients were PPE (10 UPPE and 20 CPPE) and 143 were NOT PPE...
September 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28162855/histopathological-and-scanning-electron-microscopy-findings-of-retrieved-porous-polyethylene-implants
#14
S-Y Choi, H-I Shin, T-Y Kwon, T-G Kwon
Porous polyethylene (PPE) implants are biocompatible alloplastic materials commonly used for facial augmentation. However, the effect of sub-periosteal PPE application on the surrounding tissues has not been analyzed clearly. This report documents the case of a 22-year-old woman who underwent peri-alar augmentation with PPE to improve midface retrusion. Although no infection or inflammation occurred at the surgical site, the patient requested removal of the PPE implant for aesthetic reasons alone at 1 year after the surgery...
February 2, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28153804/putrescine-essential-factor-for-in-vitro-proliferation-of-babesia-bovis
#15
C Rojas-Martínez, R I Rodríguez-Vivas, J V Figueroa Millán, K Y Acosta Viana, E J Gutiérrez Ruiz, J A Álvarez Martínez
This study reports the effect of putrescine addition, either alone or in combination with insulin, transferrin and selenite (ITS), to serum-free Advanced DMEM/F12 (A-DMEM/F12) medium, on the in vitro culture of Babesia bovis and using a perfusion bioreactor to improve efficiency of the process. A B. bovis strain previously adapted to proliferate in serum-free medium (Bbovis-SF) was evaluated using eight increasing concentrations of putrescine. The percentage of parasitized erythrocytes (PPE) obtained from cultures supplemented with 0...
January 30, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28153580/from-planar-cell-polarity-to-ciliogenesis-and-back-the-curious-tale-of-the-ppe-and-cplane-proteins
#16
REVIEW
Paul N Adler, John B Wallingford
Why some genes are more popular than others remains an open question, but one example of this phenomenon involves the genes controlling planar cell polarity (PCP), the polarization of cells within a plane of a tissue. Indeed, the so-called 'core' PCP genes such as dishevelled, frizzled, and prickle have been extensively studied both in animal models and by human genetics. By contrast, other genes that influence PCP signaling have received far less attention. Among the latter are inturned, fuzzy, and fritz, but recent work should bring these once obscure regulators into the limelight...
January 30, 2017: Trends in Cell Biology
https://www.readbyqxmd.com/read/28130364/diverse-effects-of-mycobacterial-ppe-proteins-upon-interaction-with-host-macrophages
#17
Lu Meng, Jingfeng Tong, Qinglan Wang, Chen Niu, Qian Gao
The proline-proline-glutamic acid (PPE) family proteins are abundant only in pathogenic Mycobacteria, but their general functions are far from unveiled. To investigate their roles in how Mycobacterium tuberculosis (Mtb) resists killing by the host, 25 PPE recombinant Mycobacterium smegmatis strains that overexpress Mtb PPE proteins were constructed. During phagocytosis, a similar amount of intracellular bacteria was observed at two hours post-infection (hpi) for 24 PPE recombinants, while a 50% reduction of entrance was observed for the PPE29 recombinant...
January 26, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28119467/ppe-surface-proteins-are-required-for-heme-utilization-by-mycobacterium-tuberculosis
#18
Avishek Mitra, Alexander Speer, Kan Lin, Sabine Ehrt, Michael Niederweis
: Iron is essential for replication of Mycobacterium tuberculosis, but iron is efficiently sequestered in the human host during infection. Heme constitutes the largest iron reservoir in the human body and is utilized by many bacterial pathogens as an iron source. While heme acquisition is well studied in other bacterial pathogens, little is known in M. tuberculosis To identify proteins involved in heme utilization by M. tuberculosis, a transposon mutant library was screened for resistance to the toxic heme analog gallium(III)-porphyrin (Ga-PIX)...
January 24, 2017: MBio
https://www.readbyqxmd.com/read/28112981/effect-of-porcine-placental-extract-on-the-mild-menopausal-symptoms-of-climacteric-women
#19
M Kitanohara, T Yamamoto, S Masunaga, M Ohishi, Y Komatsu, M Nagase
OBJECTIVES: This study assessed the effects of oral porcine placental extract (PPE) on the mild menopausal symptoms of climacteric women. METHODS: In this 12-week, multicenter, randomized, double-blind, placebo-controlled, parallel-group study, 50 climacteric Japanese women were randomized 1 : 1 to oral PPE (300 mg/day) or placebo. Menopausal symptoms were evaluated by using the Simplified Menopausal Index (SMI), as were serum estradiol (E2) and follicle stimulating hormone (FSH) levels...
January 23, 2017: Climacteric: the Journal of the International Menopause Society
https://www.readbyqxmd.com/read/28112562/determination-of-orientation-and-practice-requirements-when-using-an-obstacle-course-for-mobility-performance-assessment
#20
Thomas Karakolis, Brittany A Sinclair, Alison Kelly, Phil Terhaar, Linda L M Bossi
OBJECTIVE: Determine effect of orientation (introduction and familiarization) and practice (repeated performance) on human performance under various load conditions as assessed by an obstacle course. BACKGROUND: Obstacle courses are commonly used as screening tools by military, police, and firefighters or to assess human capabilities and the effect of wearing personal protective equipment (PPE) and other occupationally necessary equipment on mobility task performance...
January 1, 2017: Human Factors
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