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Hao Wu, Yue Zhao, Yuhui Du, Sen Miao, Jingui Liu, Yanni Li, Qinggele Caiyin, Jianjun Qiao
Lactococcus lactis encounters 3 environmental stimuli, H+ , lactate, and undissociated lactic acid, because of the accumulation of lactic acid-the predominant fermentation product. Few studies have examined how these stimuli synergistically affect the bacteria. Herein, we analyzed the dissociation degree of lactic acid at different pH and investigated the cellular response to cross-stress in L. lactis ssp. lactis F44 through quantitative proteomic analysis using isobaric tags for relative and absolute quantitation of 3 groups: 0% lactic acid with pH 4...
May 16, 2018: Journal of Dairy Science
Li-Qin Li, Lun Liu, Wei Zhuo, Qian Chen, Sheng Hu, Shuang Peng, Xi-Yao Wang, Yi-Fei Lu, Li-Ming Lu
Physiological and iTRAQ based proteomic analysis provided new insights into potassium deficiency stress response in alligator weed root. Alligator weed (Alternanthera philoxeroides) has a strong ability to adapt to potassium deficiency (LK) stress. Proteomic changes in response to this stress are largely unknown in alligator weed. In this study, we investigated physiological and molecular mechanisms under LK using isobaric tags for relative and absolute quantitation to characterize proteome-level changes in this plant...
May 18, 2018: Plant Molecular Biology
Daisuke Yuhara, Paul E Brumby, David T Wu, Amadeu K Sum, Kenji Yasuoka
To develop prediction methods of three-phase equilibrium (coexistence) conditions of methane hydrate by molecular simulations, we examined the use of NVT (isometric-isothermal) molecular dynamics (MD) simulations. NVT MD simulations of coexisting solid hydrate, liquid water, and vapor methane phases were performed at four different temperatures, namely, 285, 290, 295, and 300 K. NVT simulations do not require complex pressure control schemes in multi-phase systems, and the growth or dissociation of the hydrate phase can lead to significant pressure changes in the approach toward equilibrium conditions...
May 14, 2018: Journal of Chemical Physics
Andreas Singraber, Tobias Morawietz, Jörg Behler, Christoph Dellago
Using molecular dynamics simulations based on ab initio trained high-dimensional neural network potentials, we study the equation of state of liquid water at negative pressures. From density isobars computed for various pressures down to p = -230 MPa we determine the line of density maxima for two potentials based on the BLYP and the RPBE functionals, respectively. In both cases, dispersion corrections are included to account for non-local long-range correlations that give rise to van der Waals forces. We find that the density maximum persists down to the most negative pressures close to the spinodal instability...
May 15, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
Dolores Garcia-Toral, Minerva Gonzalez Melchor, Juan Francisco Rivas-Silva, Efrain Meneses-Juarez, Jose Cano-Ordaz, Gregorio Hernandez Cocoletzi
Classical molecular dynamics (MD) and density functional theory (DFT) calculations are developed to investigate the dopamine and caffeine encapsulation within boron nitride (BN) nanotubes (NT) with (14, 0) chirality. Classical MD studies are done at canonical and isobaric-isothermal conditions at 298 K and 1 bar in explicit water. Results reveal that both molecules are attracted by the nanotube, however only dopamine is able to enter the nanotube while the caffeine moves at the vicinity of it, suggesting that both species can be transported: the first by encapsulation and the second by drag...
May 15, 2018: Journal of Physical Chemistry. B
Ying Guo, Zhonghou Han, Li Guo, Yu Liu, Gang Li, Huiqing Li, Jie Zhang, Liwei Bai, Hongli Wu, Biliang Chen
Gestational Diabetes Mellitus (GDM) has brought great harm to maternal and fetus. Up to now, only a few plasma biomarkers for its early diagnosis have been reported; nevertheless, there is no report about identification of urinary biomarkers for prediction of GDM. Thus, it is necessary to correct this deficiency. In our study, urine samples were collected from 889 healthy young gravidae at the early second trimester (15 to 20 weeks), 69 of whom were subsequently diagnosed with GDM at 24 to 28 weeks. iTRAQ (the isobaric tags for relative and absolute quantification) quantitative proteomics was conducted on sixteen GDM (trial group) and an equal number of matched healthy young gravidae (control group)...
May 12, 2018: Endocrine Journal
S Noji, H Sakai, N Aoi, H Baba, G P A Berg, P Doornenbal, M Dozono, N Fukuda, N Inabe, D Kameda, T Kawabata, S Kawase, Y Kikuchi, K Kisamori, T Kubo, Y Maeda, H Matsubara, S Michimasa, K Miki, H Miya, H Miyasako, S Sakaguchi, Y Sasamoto, S Shimoura, M Takaki, H Takeda, S Takeuchi, H Tokieda, T Ohnishi, S Ota, T Uesaka, H Wang, K Yako, Y Yanagisawa, N Yokota, K Yoshida, R G T Zegers
The (^{12}N, ^{12}C) charge-exchange reaction at 175  MeV/u was developed as a novel probe for studying the isovector spin giant monopole resonance (IVSMR), whose properties are important for better understanding the bulk properties of nuclei and asymmetric nuclear matter. This probe, now available through the production of ^{12}N as a secondary rare-isotope beam, is exothermic, is strongly absorbed at the surface of the target nucleus, and provides selectivity for spin-transfer excitations. All three properties enhance the excitation of the IVSMR compared to other, primarily light-ion, probes, which have been used to study the IVSMR thus far...
April 27, 2018: Physical Review Letters
Peng Deng, Jianchun Zeng, Jie Li, Wenjun Feng, Jinlun Chen, Yirong Zeng
Avascular necrosis of the femoral head (ANFH) is an a frequently occurring orthopaedic disease with high morbidity. Traditional Chinese Medicine (TCM) Yuanshi Shengmai Chenggu Tablet is a valid prescription for treating steroid-induced femoral head necrosis. However, there are rare investigations about the serum protein marker expression after the acting of drugs on hormone and TCM. In the present study, we aimed to systematically discover and validate the serum biomarkers expression difference in patients with steroid-induced avascular necrosis of femoral head (SANFH) after taking Yuanshi Shengmai Chenggu Tablets (SANFH-TCM), so as to reveal the action mechanism of TCM from the molecular level by using isobaric tags for relative and absolute quantification (iTRAQ) with multiple reaction monitoring quantification...
2018: BioMed Research International
Qing Lin, Shifang Peng, Yixuan Yang
Metastasis is a characteristic of malignant tumors and may be a fatal clinical factor for many patients with cancer. Hepatocellular carcinoma (HCC) cells are highly metastatic; the mechanism of metastasis is complicated and may be influenced by a number of factors. Membrane proteins may block receptors or inhibit important enzymes, thus inhibiting tumor progression, and may be potential therapeutic targets for tumor prognosis and treatment. The present study aimed to use proteomics to analyze the dynamic changes of membrane proteins in HCC cells, to improve our understanding of membrane protein functions and to clarify the important components of the mechanisms of HCC metastasis...
April 26, 2018: International Journal of Oncology
Pascal Meuret, Lionel Bouvet, Benoit Villet, Mohamed Hafez, Bernard Allaouchiche, Emmanuel Boselli
Objective: Intraoperative hypotension during hip fracture surgery is frequent in the elderly. No study has compared the haemodynamic effect of hypobaric unilateral spinal anaesthesia (HUSA) and standardised general anaesthesia (GA) in elderly patients undergoing hip fracture surgical repair. Methods: We performed a prospective, randomised open study, including 40 patients aged over 75 years, comparing the haemodynamic effects of HUSA (5 mg isobaric bupivacaine with 5 μg sufentanil and 1 mL sterile water) and GA (induction with etomidate/remifentanil and maintenance with desflurane/remifentanil)...
April 2018: Turkish Journal of Anaesthesiology and Reanimation
Sonam Vijay, Ritu Rawal, Kavita Kadian, Jagbir Singh, Tridibesh Adak, Arun Sharma
BACKGROUND: Midgut invasion, a major bottleneck for malaria parasites transmission is considered as a potential target for vector-parasite interaction studies. New intervention strategies are required to explore the midgut proteins and their potential role in refractoriness for malaria control in Anopheles mosquitoes. To better understand the midgut functional proteins of An. culicifacies susceptible and refractory species, proteomic approaches coupled with bioinformatics analysis is an effective means in order to understand the mechanism of refractoriness...
May 8, 2018: BMC Genomics
José Navarrete-Perea, Qing Yu, Steven P Gygi, Joao A Paulo
Mass spectrometry (MS) coupled toisobaric labeling has developed rapidly into a powerful strategy for high-throughput protein quantification. Sample multiplexing and exceptional sensitivity allow for the quantification of tens of thousands of peptides and, by inference, thousands of proteins from multiple samples in a single MS experiment. Accurate quantification demands a consistent and robust sample-preparation strategy. Here, we present a detailed workflow for SPS-MS3-based quantitative abundance profiling of tandem mass tag (TMT)-labeled proteins and phosphopeptides that we have named the streamlined (SL)-TMT protocol...
May 16, 2018: Journal of Proteome Research
Shiwei Wu, Lujun Yu, Xiaozhe Fu, Xi Yan, Qiang Lin, Lihui Liu, Hongru Liang, Ningqiu Li
Infectious spleen and kidney necrosis virus (ISKNV) has caused significant losses in the cultured mandarin fish (Siniperca chuatsi) industry. The molecular mechanisms that underlie interaction between ISKNV and hosts are not fully understood. In this study, the proteomic profile of CPB cells at progressive time points after ISKNV infection was analyzed by isobaric tags for relative and absolute quantitation (iTRAQ). A total of 2731 proteins corresponding to 6363 novel peptides (false discovery rate <0.01) were identified...
May 4, 2018: Fish & Shellfish Immunology
Marcus Stuckenholz, Emanuel A Crespo, Lourdes F Vega, Pedro Jorge Carvalho, Joao A P Coutinho, Wolffram H Schroer, Johannes Kiefer, Bernd Rathke
Isobaric vapor liquid equilibria (VLE) of binary mixtures of the ionic liquid (IL) 1-butyl-3-methylimidazolium trifluoromethanesulfonate (C4mimTfO) with either water or short chained n-alkyl alcohols (methanol, ethanol, propan-1-ol and butan-1-ol) are described in this study. Two different microebulliometers and a classical VLE apparatus were compared and the VLEs were determined on the composition range 0.4 ≤ x(solvent) ≤ 1 at three different pressure levels (p = 500 mbar, 700 mbar and 1000 mbar). The experimental data were modeled using the soft-SAFT equation of state, which was able to accurately describe the non-ideal behavior of these mixtures...
May 7, 2018: Journal of Physical Chemistry. B
M Fairuz B Jamaluddin, Prathima B Nagendra, Pravin Nahar, Christopher Oldmeadow, Pradeep S Tanwar
Uterine leiomyomas (fibroids) are the most common gynecological tumors, which are enriched in the extracellular matrix (ECM). Fibroids are leading cause of abnormal uterine bleeding and hysterectomy. One of the major questions yet to be answered is the overproduction of specific ECM components in human uterine fibroids, particularly in relation to mutations in the driver gene mediator complex subunit 12 ( MED12). Surgical specimens from 14 patients with uterine leiomyoma having fibroids and corresponding adjacent normal myometrium (ANM) were utilized to analyze genetic and proteomic expression patterns in the tissue samples...
January 1, 2018: Reproductive Sciences
Pengjun Jiang, Jun Wu, Xin Chen, Bo Ning, Qingyuan Liu, Zhengsong Li, Maogui Li, Fan Yang, Yong Cao, Rong Wang, Shuo Wang
The underlying pathophysiological mechanisms involved in cerebral aneurysms rupture remain unclear. This study was performed to investigate the differentially expressed proteins between ruptured and unruptured aneurysms using quantitative proteomics. The aneurysmal walls of six ruptured aneurysms and six unruptured aneurysms were collected during the surgical operation. The isobaric tags for relative and absolute quantification (iTRAQ) were used to identify the differentially expressed proteins and western blotting was performed to validate the expression of the proteins of interest...
May 3, 2018: Journal of Proteomics
Xin-Ying Sun, Qing-Hui Liu, Jie Huang
The isobaric tags for relative and absolute quantitation (iTRAQ) was applied to identify differentially expressed proteins (DEPs) in Litopenaeus vannamei in response to different virulence white spot syndrome virus infection. A total of 2,780 unique peptides corresponding to 754 proteins were identified. The number of significant differentially expressed proteins was 161, including 38 up-regulated ones and 123 down-regulated ones in low-virulence infection library compared with normal-virulence infection library...
May 4, 2018: Letters in Applied Microbiology
Yu-Ping Guo, En-Zhong Li, You-Jing Zhang, An-Li Wang
Objective: To investigate the effect of aerobic exercise on the spermatogenic function of male rats and screen out differentially expressed proteins related to spermatonesis-regulation by proteomic analysis. METHODS: We randomly divided 24 SD male rats into groups A (non-exercise control), B (exercise), and C (weight-bearing exercise), those in the latter two groups made to swim for 60 minutes a day and those in group C bearing a load 3% of the body weight, both 6 times a week for 9 weeks...
September 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
Gaoming Zhang, Jiali Ye, Yulin Jia, Lingli Zhang, Xiyue Song
A &ldquo;two-line hybrid system&rdquo; was developed, previously based on thermo-sensitive cytoplasmic male sterility in Aegilops kotschyi (K-TCMS), which can be used in wheat breeding. The K-TCMS line exhibits complete male sterility and it can be used to produce hybrid wheat seeds during the normal wheat-growing season; it propagates via self-pollination at high temperatures. Isobaric tags for relative and absolute quantification-based quantitative proteome and bioinformatics analyses of the TCMS line KTM3315A were conducted under different fertility conditions to understand the mechanisms of fertility conversion in the pollen development stages...
May 2, 2018: International Journal of Molecular Sciences
Lisa B E Shields, Vasudeva G Iyer, Yi Ping Zhang, Christopher B Shields
Background: Cauda equina syndrome (CES) is a rare complication of spinal or epidural anesthesia. It is attributed to direct mechanical injury to the spinal roots of the cauda equina that may result in saddle anesthesia and paraplegia with bowel and bladder dysfunction. Case Description: The first patient underwent a hip replacement and received 5 mL of 1% lidocaine epidural anesthesia. Postoperatively, when the patient developed an acute CES, the lumbar magnetic resonance imaging (MRI) scan demonstrated clumping/posterior displacement of nerve roots of the cauda equina consistent with adhesive arachnoiditis attributed to the patient's previous L4-L5 lumbar decompression/fusion...
2018: Surgical Neurology International
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