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https://www.readbyqxmd.com/read/28532730/readiness-for-and-predictors-of-evidence-base-practice-in-greek-healthcare-settings
#1
Athina E Patelarou, Aggelos Laliotis, Hero Brokalaki, John Petrakis, Vassilis Dafermos, Evmorfia Koukia
BACKGROUND: Implementation of evidence-based practice (EBP) remains limited in healthcare settings and knowledge of predictors of healthcare professionals' EBP activities is lacking. AIM: Describe nurses' readiness for EBP and identify related predictors in Greek healthcare settings we conducted a survey. RESULTS: Nurses scored high in the EBP readiness scale reflecting significant positive readiness toward EBP and consistently reported favourable attitudes toward and beliefs about EBP...
June 2017: Applied Nursing Research: ANR
https://www.readbyqxmd.com/read/28525898/ginsenoside-rg3-inhibits-the-senescence-of-prostate-stromal-cells-through-down-regulation-of-interleukin-8-expression
#2
Yanfei Peng, Ran Zhang, Lingfei Kong, Yongmei Shen, Da Xu, Fang Zheng, Jianwei Liu, Qian Wu, Bona Jia, Ju Zhang
Senescent stromal cells support the development of prostate cancer and are considered potential therapeutic targets. This research evaluated the regulatory effects of ginsenoside Rg3 on the senescence of prostatic stromal cells pre-incubated in medium supplemented with 0.5% fetal bovine serum. The results revealed that ginsenoside Rg3 decreased the number of stromal cells positively stained with a senescent cell marker (senescence-associated β-galactosidase). Ginsenoside Rg3 also increased the viability of stromal cells and promoted cell cycle transition from G0/G1 to S phase, as well as inhibited the carcinoma-associated fibroblast-like phenotype in prostate stromal cells, through the up-regulation of smooth muscle cell markers SM22 and smooth muscle myosin heavy chain...
May 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28522833/parkin-regulation-of-chop-modulates-susceptibility-to-cardiac-endoplasmic-reticulum-stress
#3
Kim Han, Shahin Hassanzadeh, Komudi Singh, Sara Menazza, Tiffany T Nguyen, Mark V Stevens, An Nguyen, Hong San, Stasia A Anderson, Yongshun Lin, Jizhong Zou, Elizabeth Murphy, Michael N Sack
The regulatory control of cardiac endoplasmic reticulum (ER) stress is incompletely characterized. As ER stress signaling upregulates the E3-ubiquitin ligase Parkin, we investigated the role of Parkin in cardiac ER stress. Parkin knockout mice exposed to aortic constriction-induced cardiac pressure-overload or in response to systemic tunicamycin (TM) developed adverse ventricular remodeling with excessive levels of the ER regulatory C/EBP homologous protein CHOP. CHOP was identified as a Parkin substrate and its turnover was Parkin-dose and proteasome-dependent...
May 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28515388/nitric-oxide-is-critical-for-avoiding-hepatic-lipid-overloading-via-il6-induction-during-liver-regeneration-after-partial-hepatectomy-in-mice
#4
Yue Yu, Miho Tamai, Yoh-Ichi Tagawa
Nitric oxide (NO), generated from L-arginine by three different isoforms of nitric oxide synthase (NOS), is a pleiotropic factor to regulate physiological functions in almost every organ and tissue. Each knockout mouse of iNOS or eNOS has been used to suggest that NO has a crucial role in liver regeneration after partial hepatectomy (PH), for NO may inhibit caspase 3 activity and is required for EGFR signaling. In previous reports, defective mitochondrial β-oxidation was observed in eNOS KO mice, and hepatic steatosis was often correlated to deficient liver regeneration, so we focused on metabolic perspective and hypothesized that NO depletion in PH mice would affect hepatocytic lipolysis and impair hepatocytes proliferation...
May 18, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28513228/relationship-between-chop-gadd153-and-unstable-human-carotid-atherosclerotic-plaque
#5
Suping Wang, Meiyan Zhang, Zanhua Liu, Wuyang Yang, Junwei Shi, Victor Dean, Dong Chen
BACKGROUND AND AIMS: The signaling protein C/EBP homologous protein (CHOP) and corresponding growth-arrest-and-DNA-damage-inducible gene 153 (GADD153) is associated with endoplasmic reticulum stress (ERS), which can lead to apoptosis. Our study aims to elucidate the role of CHOP/GADD153 in unstable atherosclerotic (AS) plaque formation isolated from confounding factors such as diabetes mellitus, primary hyperlipidemia, autoimmune deficiencies/abnormalities, essential hypertension, chronic heart failure, chronic kidney disease, and smoking...
May 17, 2017: British Journal of Neurosurgery
https://www.readbyqxmd.com/read/28510249/mechanism-of-pingyangmycin-induced-apoptosis-of-cultured-human-umbilical-vein-endothelial-cells
#6
Y D Huang, X Tong, Q Chen, S W Xia, Y Zhuo, Z H Kang, X Y Xue
In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK-8) was used to detect growth inhibiting effects. Annexin V-FITC/propidium iodide stain was used to detect apoptosis, and western blot was used to detect the expression of glucose-related protein 78 (GPR78) and C/EBP homologous protein (CHOP) endoplasmic reticulum stress proteins...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28509809/evidence-based-practice-among-health-professionals-in-hospitals-of-northwest-ethiopia-a-cross-sectional-study
#7
Miftah A Beshir, Solomon A Woreta, Mihiretu Kebede
BACKGROUND: Evidence-based practice (EBP) is a problem-solving approach to patient care based on the best available and valid evidence. It is accentuated to increase the quality of care and patient safety. EBP in clinical service is low in developing countries including Ethiopia. This study aimed at assessing EBP and associated factors among health professionals in North Gondar Administrative Zone hospitals, Northwest Ethiopia. METHODS: Institution-based cross-sectional study was carried out from March to May 2015 in three hospitals in North Gondar Zone...
May 15, 2017: International Journal of Evidence-based Healthcare
https://www.readbyqxmd.com/read/28507169/human-neutrophil-elastase-induces-endothelial-cell-apoptosis-by-activating-the-perk-chop-branch-of-the-unfolded-protein-response
#8
Irina Grechowa, Sven Horke, Anja Wallrath, Christian-Friedrich Vahl, Bernhard Dorweiler
Human neutrophil elastase impacts on atherosclerotic plaque stability by inducing apoptosis in endothelial cells. Our aim was to investigate the proapoptotic mechanism of elastase on endothelial cells and to evaluate the presence of elastase in human plaque material. Human endothelial cells were treated with purified human neutrophil elastase. Apoptosis was assayed by capsase-3/7 activation, TUNEL, and sub-G1 assay. Activation of unfolded protein response (UPR) effector molecules binding Ig protein, soluble X-binding protein-1, protein kinase RNA-like ER kinase (PERK), and C/EBP-homologous protein (CHOP) was analyzed by RT-PCR, immunocytochemistry, and Western blot...
May 15, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28505839/explosive-percolation-on-a-scale-free-multifractal-weighted-planar-stochastic-lattice
#9
M M Rahman, M K Hassan
In this article, we investigate explosive bond percolation (EBP) with the product rule, formally known as the Achlioptas process, on a scale-free multifractal weighted planar stochastic lattice. One of the key features of the EBP transition is the delay, compared to the corresponding random bond percolation (RBP), in the onset of the spanning cluster. However, when it happens, it happens so dramatically that initially it was believed, although ultimately proved wrong, that explosive percolation (EP) exhibits a first-order transition...
April 2017: Physical Review. E
https://www.readbyqxmd.com/read/28501567/depletion-of-jmjd1c-impairs-adipogenesis-in-murine-3t3-l1-cells
#10
Florian Buerger, Silvana Müller, Nadja Ney, Juliane Weiner, John T Heiker, Sonja Kallendrusch, Peter Kovacs, Dorit Schleinitz, Joachim Thiery, Sonja C Stadler, Ralph Burkhardt
Differentiation of adipocytes is a highly regulated process modulated by multiple transcriptional co-activators and co-repressors. JMJD1C belongs to the family of jumonji C (jmjC) domain-containing histone demethylases and was originally described as a ligand-dependent co-activator of thyroid hormone and androgen receptors. Here, we explored the potential role of Jmjd1c in white adipocyte differentiation. To investigate the relevance of Jmjd1c in adipogenesis, murine 3T3-L1 preadipocyte cells with transient knock-down of Jmjd1c (3T3_Jmjd1c) were generated...
May 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28499401/are-general-and-strategic-measures-of-organizational-context-and-leadership-associated-with-knowledge-and-attitudes-toward-evidence-based-practices-in-public-behavioral-health-settings-a-cross-sectional-observational-study
#11
Byron J Powell, David S Mandell, Trevor R Hadley, Ronnie M Rubin, Arthur C Evans, Matthew O Hurford, Rinad S Beidas
BACKGROUND: Examining the role of modifiable barriers and facilitators is a necessary step toward developing effective implementation strategies. This study examines whether both general (organizational culture, organizational climate, and transformational leadership) and strategic (implementation climate and implementation leadership) organizational-level factors predict therapist-level determinants of implementation (knowledge of and attitudes toward evidence-based practices). METHODS: Within the context of a system-wide effort to increase the use of evidence-based practices (EBPs) and recovery-oriented care, we conducted an observational, cross-sectional study of 19 child-serving agencies in the City of Philadelphia, including 23 sites, 130 therapists, 36 supervisors, and 22 executive administrators...
May 12, 2017: Implementation Science: IS
https://www.readbyqxmd.com/read/28499237/the-protective-effect-of-propofol-against-tnf-%C3%AE-induced-apoptosis-was-mediated-via-inhibiting-inos-no-production-and-maintaining-intracellular-ca-2-homeostasis-in-mouse-hippocampal-ht22-cells
#12
Zheng Xu, Yan Lu, Jiaqiang Wang, Xiaowei Ding, Jiawei Chen, Changhong Miao
AIM: Inflammation cytokine tumor necrosis factor-α (TNF-α) induces apoptosis in neuronal cells. We hypothesized that propofol may attenuate TNF-α-induced apoptosis in mouse hippocampal HT22 cells and aimed to explore the underlying mechanisms. METHODS: Mouse hippocampal HT22 cells were pretreated with propofol, and then stimulated with TNF-α. Cell viability was measured by cell counting kit 8 (CCK8). Cell apoptosis was examined by flow cytometry analysis. The effect of propofol on TNF-α-modulated nitric oxide production was measured by a nitrate reductase assay kit, intracellular calcium release and mitochondrial membrane potential (MMP) depolarization were measured by flow cytometry analysis, and the expression of inducible nitric oxide synthase (iNOS), C/EBP homologous protein (CHOP), B-cell lymphoma 2 (Bcl2) family and caspases were detected by Western blot...
May 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28495142/n-methyl-d-aspartate-receptor-mediated-calcium-overload-and-endoplasmic-reticulum-stress-are-involved-in-interleukin-1beta-induced-neuronal-apoptosis-in-rat-hippocampus
#13
Yilong Dong, Allan V Kalueff, Cai Song
Increased levels of interleukin (IL)-1β and its gene expression are implicated in the etiology of Alzheimer's disease (AD). IL-1β activates microglia and stimulates glutamatergic N-methyl-d-aspartate receptor NMDA receptor expression, thereby disturbing intracellular Ca(2+) homeostasis. Ca(2+) disequilibrium, in turn, may trigger endoplasmic reticulum (ER) stress, contributing to overall excitotoxicity and neuronal death that evoke AD. However, it is unclear whether IL-1β-induced neuronal apoptosis is mediated by the glutamatergic system, ER stress and/or Ca(2+) dysfunction...
June 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28494841/quercetin-attenuates-benzo-%C3%AE-pyrene-induced-cyp1a-expression
#14
M L Perepechaeva, T A Seredina, Y A Sidorova, E N Pivovarova, A L Markel, V V Lyakhovich, A Y Grishanova
We studied effects of nutrient quercetin on cytochromes' Р450 1А (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities)...
April 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28490236/cinnamyl-alcohol-the-bioactive-component-of-chestnut-flower-absolute-inhibits-adipocyte-differentiation-in-3t3-l1-cells-by-downregulating-adipogenic-transcription-factors
#15
Dae Il Hwang, Kyung-Jong Won, Do-Yoon Kim, Bokyung Kim, Hwan Myung Lee
The extract of chestnut (Castanea crenata var. dulcis) flower (CCDF) has antioxidant and antimelanogenic properties, but its anti-obesity properties have not been previously examined. In this study, we tested the effect of CCDF absolute on adipocyte differentiation by using 3T3-L1 cells and determining the bioactive component of CCDF absolute in 3T3-L1 cell differentiation. CCDF absolute (0.1-100[Formula: see text][Formula: see text]g/mL) did not change 3T3-L1 cell viability. At 50[Formula: see text][Formula: see text]g/mL and 100[Formula: see text][Formula: see text]g/mL, the absolute significantly reduced the accumulation of lipid droplets in 3T3-L1 cells that were induced by culture in medium containing 3-isobutyl-1-methylxanthine/dexamethasone/insulin (MDI)...
May 11, 2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28487965/ho-1-ebp-interaction-alleviates-cholesterol-induced-hypoxia-through-the-activation-of-the-akt-and-nrf2-mtor-pathways-and-inhibition-of-carbohydrate-metabolism-in-cardiomyocytes
#16
Xiaohan Jin, Zhongwei Xu, Jin Cao, Rui Yan, Ruicheng Xu, Ruiqiong Ran, Yongqiang Ma, Wei Cai, Rong Fan, Yan Zhang, Xin Zhou, Yuming Li
Heme oxygenase-1 (HO-1) is an inducible and cytoprotective enzyme that provides a defense against oxidant damage. The present study screened 137 HO-1/interacting proteins using a profound co-immunoprecipitation (Co-IP) coupled with proteomics, and profiled the global HO-1 interactome network, including oxidative phosphorylation, endoplasmic reticulum and transport vesicle functions. Among these molecules, we observed that a novel interactor, emopamil-binding protein (EBP), is closely related to the cholesterol metabolism process...
June 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28486533/health-sciences-evidence-based-practice-questionnaire-hs-ebp-for-measuring-transprofessional-evidence-based-practice-creation-development-and-psychometric-validation
#17
Juan Carlos Fernández-Domínguez, Joan Ernest de Pedro-Gómez, José Miguel Morales-Asencio, Miquel Bennasar-Veny, Pedro Sastre-Fullana, Albert Sesé-Abad
INTRODUCTION: Most of the EBP measuring instruments available to date present limitations both in the operationalisation of the construct and also in the rigour of their psychometric development, as revealed in the literature review performed. The aim of this paper is to provide rigorous and adequate reliability and validity evidence of the scores of a new transdisciplinary psychometric tool, the Health Sciences Evidence-Based Practice (HS-EBP), for measuring the construct EBP in Health Sciences professionals...
2017: PloS One
https://www.readbyqxmd.com/read/28485890/inhibition-of-endoplasmic-reticulum-stress-apoptosis-by-estrogen-protects-human-umbilical-vein-endothelial-cells-through-the-pi3-kinase-akt-signaling-pathway
#18
Yu Wang, Xin Yang, Xiao-Dong Li, Yong-Fen Qi, Xiao-Jing He, Yan-Jie Wang
OBJECTIVES: We aimed to investigate whether the cardioprotective effect of estrogen is mediated by inhibiting the apoptosis induced by endoplasmic reticulum stress (ERS) and to explore the underlying signaling pathway responsible for this effect. METHODS: The effect of estrogen on ERS apoptosis, the mechanism responsible for that effect, and the ERS signaling pathways were examined in human umbilical vein endothelial cells (HUVECs) and measured using western blot or Hoechst stains...
May 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28484500/pingmu-decoction-induces-orbital-preadipocytes-apoptosis-in-vitro
#19
Yali Zhang, Hong Li, Long Gao, Xia Zhang, RuiFang Xie
Pingmu Decoction is the Traditional Chinese Medicine which has treated Graves' Ophthalmopathy (GO) in the inactive stage for more than ten years. This study was to explore the mechanism of Pingmu Decoction of inhibiting preadipocytes in GO patients from differentiating into mature adipocytes. Human orbital preadipocytes were isolated and cultured through tissue explant method. Orbital preadipocytes were induced into mature adipocytes. The medicinal serum was prepared from rats. The cells were treated with medicinal serum which were divided into three groups, low dose group (5%), medium dose group (10%), and high dose group (20%)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28482410/-therapeutic-efficacy-and-mechanism-of-action-of-ginsenoside-rg1-in-treating-acute-hepatic-failure-in-mice
#20
H Luo, W X Huang, C Yang, J Q Zhao, S Liu, Y S Xu, C W Liu
Objective: To examine the regulatory effect of ginsenoside Rg1 (G-Rg1) on endoplasmic reticulum stress and its effect on hepatocellular apoptosis in carbon tetrachloride (CCl(4))-induced acute liver failure (ALF). Methods: Forty healthy, adult male C57/BL mice were randomly divided into normal saline control (NS) group, G-Rg1 blank control (G-Rg1) group, CCl(4) model (CCl(4)) group, and G-Rg1 preventive treatment (CCl(4)+G-Rg1) group, and an ALF mouse model was established by CCl(4) induction. Blood and liver specimens were collected from all mice upon sacrifice at 12 hours post-intraperitoneal injection...
March 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
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