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Eileen J Carter, Reynaldo R Rivera, Kelly A Gallagher, Kenrick D Cato
OBJECTIVES: The aim of this study is to assist nurse leaders in developing innovative structures to foster a culture of inquiry among professional nurses. BACKGROUND: Critical to nurse's engagement in evidence-based practice (EBP) is a culture of inquiry, in which nurses critically evaluate patient care activities and actively review existing evidence to address identified clinical issues. A bundle of structural interventions was implemented across a large, multisite hospital to advance a culture of inquiry...
January 2018: Journal of Nursing Administration
Ashley T Scudder, Sarah M Taber-Thomas, Kristen Schaffner, Joy R Pemberton, Leah Hunter, Amy D Herschell
BACKGROUND: In recent decades, evidence-based practices (EBPs) have been broadly promoted in community behavioural health systems in the United States of America, yet reported EBP penetration rates remain low. Determining how to systematically sustain EBPs in complex, multi-level service systems has important implications for public health. This study examined factors impacting the sustainability of parent-child interaction therapy (PCIT) in large-scale initiatives in order to identify potential predictors of sustainment...
December 7, 2017: Health Research Policy and Systems
Hao Ding, Feng Bai, Hongdi Cao, Jing Xu, Li Fang, JiNing Wu, Qi Yuan, Yang Zhou, Qi Sun, WeiChun He, ChunSun Dai, Ke Zen, Lei Jiang, Junwei Yang
AIM: cAMP is a universal second messenger that plays an important role in intracellular signal transduction. cAMP is synthesized by adenylate cyclases (ACs) from adenosine triphosphate (ATP), and terminated by the phosphodiesterases (PDEs). In the present study, we investigated the role of the cAMP pathway in tubular epithelial cells mitochondrial biogenesis in the pathogenesis of renal fibrosis. RESULTS: We found that the cAMP levels were decreased in fibrotic kidney tissues, and replenishing cAMP could ameliorate tubular atrophy and extracellular matrix deposition...
December 7, 2017: Antioxidants & Redox Signaling
Ángeles Canales, Marcel Rösinger, Javier Sastre, Isabella C Felli, Jesús Jiménez-Barbero, Guillermo Giménez-Gallego, Carlos Fernández-Tornero
C/EBP-homologous protein (CHOP) is a key determinant of the apoptotic response to endoplasmic reticulum stress or DNA damage. As a member of the C/EBP family, CHOP contains a low complexity N-terminal region involved in transcriptional activation, followed by a bZIP that binds DNA after dimerization. However, in contrast to other C/EBPs, CHOP directs binding to non-canonical C/EBP sites due to unique substitutions in its DNA-binding domain. Herein, we show that the N-terminal region of CHOP is intrinsically unstructured but contains two segments presenting α-helical propensity...
2017: PloS One
Minji Woo, Mijeong Kim, Jeong Sook Noh, Chan Hum Park, Yeong Ok Song
BACKGROUND/OBJECTIVES: Endoplasmic reticulum (ER) stress is positively associated with atherosclerosis via elevating macrophage cell death and plaque formation, in which oxidative stress plays a pivotal role. Antioxidative, lipid-lowering, and anti-atherogenic effects of kimchi, a Korean fermented vegetable, have been established, wherein capsaicin, ascorbic acid, quercetin, 3-(4'-hydroxyl-3',5'-dimethoxyphenyl)propionic acid, and lactic acids were identified. In this study, mechanisms of action of kimchi methanol extracts (KME) on fatty streak formation via suppression of ER stress and apoptosis in aorta were examined in low-density lipoprotein receptor knockout mice...
December 2017: Nutrition Research and Practice
Nai-Qian Zhao, Xiao-Yan Li, Li Wang, Zi-Ling Feng, Xi-Fen Li, Yan-Fang Wen, Jin-Xiang Han
AIM: To determine the role of G0/G1 switch gene 2 (G0S2) and its transcriptional regulation in palmitate-induced hepatic lipid accumulation. METHODS: HepG2 cells were treated with palmitate, or palmitate in combination with CCAAT/enhancer binding protein (C/EBP)β siRNA or G0S2 siRNA. The mRNA expression of C/EBPβ, peroxisome proliferator-activated receptor (PPAR)γ and PPARγ target genes (G0S2, GPR81, GPR109A and Adipoq) was examined by qPCR. The protein expression of C/EBPβ, PPARγ, and G0S2 was determined by Western blotting...
November 21, 2017: World Journal of Gastroenterology: WJG
Lei Jing, Xue-Wen Jia
Palmitate (PA) has been identified to induce cell apoptosis in osteoblasts. The c‑Jun NH2‑teminal kinase (JNK) signaling pathway and endoplasmic reticulum stress (ERS) were found to be important contributors. Therefore, natural or synthetic agents may antagonize PA‑induced apoptosis in osteoblasts, and demonstrate the potential application to reverse osteoporosis. The present study demonstrated that the Lycium barbarum polysaccharide (LBP) is as a major active ingredient of Lycium barbarum and that it can reduce the fatty acid toxicity of PA...
November 21, 2017: Molecular Medicine Reports
Bing Wang, Chao Wu, Bo Shi, Lang Huang
INTRODUCTION: In safety management (SM), it is important to make an effective safety decision based on the reliable and sufficient safety-related information. However, many SM failures in organizations occur for a lack of the necessary safety-related information for safety decision-making. Since facts are the important basis and foundation for decision-making, more efforts to seek the best evidence relevant to a particular SM problem would lead to a more effective SM solution. Therefore, the new paradigm for decision-making named "evidence-based practice (EBP)" can hold important implications for SM, because it uses the current best evidence for effective decision-making...
December 2017: Journal of Safety Research
Mary Acri, Emily Hamovitch, Maria Mini, Elene Garay, Claire Connolly, Mary McKay
BACKGROUND: Oppositional defiant disorder (ODD) is a major mental health concern and highly prevalent among children living in poverty-impacted communities. Despite that treatments for ODD are among the most effective, few children living in poverty receive these services due to substantial barriers to access, as well as difficulties in the uptake and sustained adoption of evidence-based practices (EBPs) in community settings. The purpose of this study is to examine implementation processes that impact uptake of an evidence-based practice for childhood ODD, and the impact of a Clinic Implementation Team (CIT)-driven structured adaptation to enhance its fit within the public mental health clinic setting...
December 4, 2017: Trials
Vanessa Sovrani, Juliana da Rosa, Mariana de Paula Drewinski, Franciely Grose Colodi, Tânia Toyomi Tominaga, Herta Stutz Dalla Santa, Rosilene Rebeca
We studied antitumor activity in vitro and in vivo and partial chemical characterization of exobiopolymers (EBPs) of Agaricus brasiliensis produced by submerged cultivation. The EBPs comprised 61.2% total sugar and 22.3% total protein, and the chromatographic profile mainly identified mannose (65.5%), galactose (17.5%), and glucose (13.1%). In vitro analysis revealed a dose with antitumor effectiveness, time-dependent treatment, and 46% inhibition of solid Walker 256 tumors in rats. Metabolic changes were significant in the spleens of the animals; this is possibly related to higher immune activity triggered by the presence of EBPs in the treated group...
2017: International Journal of Medicinal Mushrooms
Myra E Woodworth-Hobbs, Ben D Perry, Jill A Rahnert, Matthew B Hudson, Bin Zheng, S Russ Price
Lipid accumulation in skeletal muscle results in dysregulation of protein metabolism and muscle atrophy. We previously reported that treating C2C12 myotubes with palmitate (PA), a saturated fatty acid, increases the overall rate of proteolysis via activation of the ubiquitin-proteasome and autophagy systems; co-treatment with the omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) prevents the PA-induced responses. Others have reported that PA induces endoplasmic reticulum (ER) stress which initiates the unfolded protein response (UPR), a collective group of responses that can lead to activation of caspase-mediated proteolysis and autophagy...
December 2017: Physiological Reports
Kaviyarasi Renu, Harishkumar Madhyastha, Radha Madhyastha, Masugi Maruyama, Sankarganesh Arunachlam, Abilash V G
Exposure to arsenic in drinking water can stimulate a diverse number of diseases that originate from impaired lipid metabolism in adipose and glucose metabolism, leading to insulin resistance. Arsenic inhibits differentiation of adipocyte and mediates insulin resistance with diminutive information on arsenicosis on lipid storage and lipolysis. This review focused on different mechanisms and pathways involved in adipogenesis and lipolysis in adipose tissue during arsenic-induced diabetes. Though arsenic is known to cause type2 diabetes through different mechanisms, the role of adipose tissue in causing type2 diabetes is still unclear...
November 30, 2017: Toxicology Letters
Seung-Eun Song, Su-Kyung Shin, Hyun-Woo Cho, Seung-Soon Im, Jae-Hoon Bae, Seon Min Woo, Taeg-Kyu Kwon, Dae-Kyu Song
In this study, we examined the effect of tomatidine on tumor necrosis factor (TNF)-α-induced apoptosis in C2C12 myoblasts. TNF-α treatment increased cleaved caspase 3 and cleaved poly (ADP-ribose) polymerase (PARP) protein levels in a dose- and time-dependent manner. Pretreatment of cells with 10 μM tomatidine prevented TNF-α-induced apoptosis, caspase 3 cleavage, and PARP cleavage. Cells were treated with 100 ng/mL TNF-α for 24 h, and flow cytometry was utilized to assess apoptosis using annexin-V and 7-aminoactinomycin D...
December 1, 2017: Molecular and Cellular Biochemistry
Hyun Yi, Shue Liu, Yuta Kashiwagi, Daigo Ikegami, Wan Huang, Hirotsugu Kanda, Takafumi Iida, Ching-Hang Liu, Keiya Takahashi, David A Lubarsky, Shuanglin Hao
Chronic pain is increasingly recognized as an important comorbidity of HIV-infected patients, however, the exact molecular mechanisms of HIV-related pain are still elusive. CCAAT/enhancer binding proteins (C/EBPs) are expressed in various tissues, including the central nervous system. C/EBPβ, one of C/EBPs is involved in the progression of HIV/AIDS, but the exact role of C/EBPβ and its upstream factors are not clear in HIV pain state. Here, we used a neuropathic pain model of perineural HIV envelope glycoprotein gp120 application onto the rat sciatic nerve to test the role of phosphorylated C/EBPβ (pC/EBPβ) and its upstream pathway in the spinal cord dorsal horn (SCDH)...
December 1, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
Guangdong Yang, Youngjun Ju, Ming Fu, Yanjie Zhang, Yanxi Pei, Mélanie Racine, Simran Baath, Thomas J S Merritt, Rui Wang, Lingyun Wu
Hydrogen sulfide (H2S) has been recognized as an important gasotransmitter analogous to nitric oxide and carbon monoxide. Cystathionine gamma-lyase (CSE)-derived H2S is implicated in the regulation of insulin resistance and glucose metabolism, but the involvement of CSE/H2S system in energy homeostasis and fat mass has not been extensively explored. In this study, a potential functional role of the CSE/H2S system in in vitro adipocyte differentiation and in vivo adipogenesis and the underlying mechanism was investigated...
November 27, 2017: Biochimica et Biophysica Acta
Per N Andersen, Kjell T Hovik, Erik W Skogli, Merete G Øie
Children with autism often struggle with emotional and behavioral problems (EBP). This study investigated whether level of autism symptoms, attention problems or verbal IQ at baseline can predict EBP 2 years later in children with High-Functioning Autism (HFA). Thirty-four participants with HFA and 45 typically developing children (TD) (ages 9-16) were assessed with parent ratings of EBP, autism symptoms, attention problems, and a test of verbal IQ. The amount of autism symptoms and degree of attention problems at baseline significantly predicted EBP at follow-up, whereas verbal IQ did not...
2017: Frontiers in Psychology
Sunyoung Park, Kyuhan Kim, Il-Hong Bae, Sung Hoon Lee, Jiyong Jung, Tae Ryong Lee, Eun-Gyung Cho
As the outermost physical barrier of an organism, the skin is diurnally exposed to UV rays (UVRs). Recent studies have revealed that the skin exhibits a circadian rhythm in various functions, and this oscillation is disturbed and reset via a strong environmental cue, the UVRs. However, a molecular link between circadian perturbation by UVRs and UVR-induced cellular responses has not been investigated. We identified tissue inhibitor of metalloproteinase (TIMP)-3 as a novel circadian locomotor output cycles kaput (CLOCK)-dependent diurnal gene by using a CLOCK-knockdown strategy in human keratinocytes...
November 27, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Stephanie M Cailor, Aleda M H Chen, Mary E Kiersma, Carrie N Keib
BACKGROUND: Pharmacists need be able to understand and utilize evidence from the literature to provide optimal patient care as well as participate in research to improve care. Thus, it is important for pharmacy students to acquire skills in research and evidence-based practice (EBP). OBJECTIVES: To evaluate the changes in pharmacy student (1) perceptions of research and EBP, (2) interest in research participation, and (3) confidence in understanding the research process and developing a research proposal after completing a research course...
January 2017: Currents in Pharmacy Teaching & Learning
Xin Yang, Suyun Qian, Kaihu Yao, Lijuan Wang, Yingchao Liu, Fang Dong, Wenqi Song, Jinghui Zhen, Wei Zhou, Hong Xu, Hongyan Zheng, Wenting Li
BACKGROUND: This study aimed to investigate the clinical and molecular epidemiology and biofilm formation of Staphylococcus aureus (SA) isolated from pediatricians in China. METHODS: SA strains were isolated from Beijing Children's hospital from February 2016 to January 2017. Isolates were typed by multilocus sequence typing (MLST), spa and SCCmec typing (for Methicillin-resistant SA [MRSA] only). Antimicrobial susceptibility testing was performed by agar dilution method except sulphamethoxazole/trimethoprim (E-test method)...
November 25, 2017: BMC Infectious Diseases
Kuei-Chang Li, Yu-Han Chang, Mu-Nung Hsu, Shih-Chun Lo, Wan-Hua Li, Yu-Chen Hu
Osteoporotic patients often suffer from bone fracture but its healing is compromised due to impaired osteogenesis potential of bone marrow-derived mesenchymal stem cells (BMSCs). Here we aimed to exploit adipose-derived stem cells from ovariectomized rats (OVX-ASCs) for bone healing. We unraveled that OVX-ASCs highly expressed miR-214 and identified 2 miR-214 targets: CTNNB1 (β-catenin) and TAB2. We demonstrated that miR-214 targeting of these two genes blocked the Wnt pathway, led to preferable adipogenesis and hindered osteogenesis...
November 24, 2017: Scientific Reports
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