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Roan Louw, Izelle Smuts, Kimmey-Li Wilsenach, Lindi-Maryn Jonck, Maryke Schoonen, Francois H van der Westhuizen
BACKGROUND: Coenzyme Q10 (CoQ10 ) is an important component of the mitochondrial respiratory chain (RC) and is critical for energy production. Although the prevalence of CoQ10 deficiency is still unknown, the general consensus is that the condition is under-diagnosed. The aim of this study was to retrospectively investigate CoQ10 deficiency in frozen muscle specimens in a cohort of ethnically diverse patients who received muscle biopsies for the investigation of a possible RC deficiency (RCD)...
February 23, 2018: Molecular Genetics and Metabolism
Yeji Sung, Konstantina Spagou, Marina Kafeza, Michael Kyriakides, Brahman Dharmarajah, Joseph Shalhoub, Jose A Diaz, Thomas W Wakefield, Elaine Holmes, Alun H Davies
OBJECTIVES: Deep vein thrombosis (DVT) is a major health problem, responsible for significant morbidity and mortality. The identification of a simple and effective diagnostic biomarker of DVT remains a challenge. Metabolomics have recently emerged as a new powerful scientific tool to characterise metabolic phenotypes of complex diseases and investigate small molecules in biofluids. The aim of the study was to identify the blood and vein wall metabolomic signature of DVT in a murine experimental model...
March 8, 2018: European Journal of Vascular and Endovascular Surgery
Maria Fotiou, Charalambos Fotakis, Foteini Tsakoumaki, Elpiniki Athanasiadou, Charikleia Kyrkou, Aristea Dimitropoulou, Thalia Tsiaka, Anastasia Chrysovalantou Chatziioannou, Kosmas Sarafidis, George Menexes, Georgios Theodoridis, Costas G Biliaderis, Panagiotis Zoumpoulakis, Apostolos P Athanasiadis, Alexandra-Maria Michaelidou
Maternal diet may influence offspring's health, even within well-nourished populations. Amniotic fluid (AF) provides a rational compartment for studies on fetal metabolism. Evidence in animal models indicates that maternal diet affects AF metabolic profile; however, data from human studies are scarce. Therefore, we have explored whether AF content may be influenced by maternal diet, using a validated food-frequency questionnaire and implementing NMR-based metabolomics. Sixty-five AF specimens, from women undergoing second-trimester amniocentesis for prenatal diagnosis, were analysed...
March 6, 2018: Scientific Reports
Liyu Peng, Lise Appels, Haijia Su
This study investigated the synergistic effect of sodium citrate (SC; Na3 C3 H5 O(COO)3 ) and microwave (MW) treatment on the efficiency of the anaerobic digestion of excess sewage sludge. In terms of the methane yield, an increase of the digestion's efficiency was observed. Taking into account the cost for the MW energy supplied to the system, the optimum treatment conditions were a MW energy input of 20 MJ/kg TS and a SC concentration of 0.11 g/g TS, obtaining a methane yield of 218.88 ml/g VS, i.e...
March 1, 2018: Journal of Environmental Management
Michael Macia, Emilie Pecchi, Martine Desrois, Carole Lan, Christophe Vilmen, Bernard Portha, Monique Bernard, David Bendahan, Benoît Giannesini
The functional and bioenergetics impact of regular physical activity upon type-2 diabetic skeletal muscle independently of confounding factors of overweight remains undocumented. Here, gastrocnemius muscle energy fluxes, mitochondrial capacity and mechanical performance were assessed noninvasively and longitudinally in non-obese diabetic Goto-Kakizaki rats using magnetic resonance (MR) imaging and dynamic 31-phosphorus MR spectroscopy (31 P-MRS) throughout a 6-min fatiguing bout of exercise performed before, in the middle (4-week) and at the end of an 8-week training protocol consisting in 60-min daily run on a treadmill...
February 27, 2018: Biochimie
Joana Carrola, Ricardo J B Pinto, Maryam Nasirpour, Carmen S R Freire, Ana M Gil, Conceição Santos, Helena Oliveira, Iola F Duarte
The expansion of biomedical and therapeutic applications of silver nanoparticles (AgNPs) raises the need to further understand their biological effects on human cells. In this work, NMR metabolomics has been applied to reveal the metabolic effects of AgNPs toward human hepatoma (HepG2) cells, which are relevant with respect to nanoparticle accumulation and detoxification. Cellular responses to widely disseminated citrate-coated AgNPs (Cit30) and to emergent biogenic AgNPs prepared using an aqueous plant extract as reducing and stabilizing agent (GS30) have been compared with a view to assess the influence of nanoparticle coating on the metabolic effects produced...
March 9, 2018: Journal of Proteome Research
Hongtao Liu, Li Jia, Wenfei Guo, Yingying Sun, Rining Zhu, Shuguang Li, Guanggang Qu, Hexiang Jiang, Junjie Wang, Jingmin Gu, Changjiang Sun, Xin Feng, Wenyu Han, Liancheng Lei
Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause meningitis both in pigs and in human beings. However, the pathogenesis of central nervous system (CNS) infection caused by SS2 have not yet been elucidated. To find the key molecules in cerebrospinal fluid (CSF) needed for the pathogenesis, a SS2 meningoencephalitic pig model and a SS2 non-meningoencephalitic pig model were established in this study. CSF was collected from infected piglets, and protein profiling was performed with label-free proteomics technology...
2018: Frontiers in Cellular and Infection Microbiology
Dohyoung Kim, Jongsung Kim
Recently various nanomaterials have been prepared using the microwave irradiation technology because of its advantages over conventional thermal decomposition methods such as the precise control of reaction temperature, lower energy consumption, and target-oriented uniform heating. In this study, water-soluble Cu-In-S/ZnS core/shell quantum dots (QDs) with average size in the range 3.5-3.7 nm were successfully synthesized in aqueous phase by microwave irradiation. L-glutathione and trisodium citrate dihydrate were used as a stabilizer between indium and copper ions...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Liang Dong, Changjun Hou, Huanbao Fa, Mei Yang, Huixiang Wu, Liang Zhang, Danqun Huo
Cartap residue poses a great threat to human health and its derivatives would remain in soils, natural waters and other environmental domains for a long time. Herein, a simple, rapid and ultrasensitive analytical method for the determination of cartap based on fluorescence resonance energy transfer (FRET) between Au nanoparticles (AuNPs) and rhodamine B (RB) is first described. With the presence of citrate-stabilized AuNPs, the fluorescence of RB was remarkably quenched by AuNPs via FRET. The fluorescence of the AuNPs-RB system was recovered upon addition of cartap, cartap can be adsorbed on the surface of AuNPs due to its amino group that has good affinity with gold, which could induce the aggregation of AuNPs accompanying color change from red to blue...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Ana M Gil, Daniela Duarte, Joana Pinto, António S Barros
This nuclear magnetic resonance metabolomics study compared the influence of two different central Portugal exposomes, one of which comprised an important source of pollutants (the Estarreja Chemical Complex, ECC), on the urinary metabolic trajectory of a cohort of healthy pregnant women (total n = 107). An exposome-independent description of pregnancy metabolism was found to comprise a set of 18 metabolites reflecting expected changes in branched-chain amino acid catabolism and hormone and lipid metabolisms...
March 2, 2018: Journal of Proteome Research
Margaux Luck, Caroline Schmitt, Neila Talbi, Laurent Gouya, Cédric Caradeuc, Hervé Puy, Gildas Bertho, Nicolas Pallet
Introduction: Metabolomic profiling combines Nuclear Magnetic Resonance spectroscopy with supervised statistical analysis that might allow to better understanding the mechanisms of a disease. Objectives: In this study, the urinary metabolic profiling of individuals with porphyrias was performed to predict different types of disease, and to propose new pathophysiological hypotheses. Methods: Urine 1H-NMR spectra of 73 patients with asymptomatic acute intermittent porphyria (aAIP) and familial or sporadic porphyria cutanea tarda (f/sPCT) were compared using a supervised rule-mining algorithm...
2018: Metabolomics: Official Journal of the Metabolomic Society
Mingyi Chen, Biyu Lu, Yuan Li, Yuanyuan Wang, Haihui Zheng, Danmin Zhong, Ziqiong Liao, Mengxia Wang, Fangli Ma, Qiongfeng Liao, Zhiyong Xie
Background: Polysaccharides can alleviate obesity in mammals; however, studies on mechanism of this alleviation are limited. A few studies have indicated that polysaccharides improve obesity by regulating the metabolism of the body. Therefore, a metabolomics approach, consisting of high resolution nuclear magnetic resonance (NMR) spectroscopy and a multivariate statistical technique, was applied to explore the mechanism of the protective effects of lentinan and Flos Lonicera polysaccharides (LF) on high-fat diet (HFD) induced obesity...
2018: Nutrition & Metabolism
Yanli Liu, Dan Cao, Linlong Ma, Xiaofang Jin, Pingfang Yang, Fei Ye, Panpan Liu, Ziming Gong, Chaoling Wei
The tea plant is a fluoride hyperaccumulator, and fluoride accumulation in its leaves is closely related to human health. To dissect molecular mechanisms underlying fluoride accumulation/detoxification, the leaves of tea seedlings exposed to different fluoride treatments for 30 days were sampled for physiological and proteomics analyses. The results showed that fluoride had no adverse effects on the growth of tea seedlings in spite of high content fluoride accumulation in their leaves. Through TMT coupled with UPLC MS/MS, 189 differentially accumulated proteins were quantified, of which 41 and 148 were localized in the cell wall and cellular compartments respectively...
February 2, 2018: Journal of Proteomics
Yin-Lin Wang, Hao-Hueng Chang, Yu-Chih Chiang, Chao-Hsiu Lin, Chun-Pin Lin
BACKGROUND/PURPOSE: Strontium ion may play a role similar to calcium ion in enamel remineralization. The aim of this study was to investigate the effect of strontium ion concentration gradient on the demineralization of enamel. METHODS: Citric acid and sodium citrate were used to prepare 16 different experimental acidic solutions with four different pH values (2.5, 3.5, 4.5, and 5.5) and four different strontium ion concentrations (0 M, 10-4 M, 10-3 M, and 10-2 M)...
January 25, 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Chao Zhu, Suxia Liang, Erhong Song, Yuanjun Zhou, Wen Wang, Feng Shan, Yantao Shi, Ce Hao, Kuibo Yin, Tong Zhang, Jianjun Liu, Haimei Zheng, Litao Sun
Inside a liquid solution, oriented attachment (OA) is now recognized to be as important a pathway to crystal growth as other, more conventional growth mechanisms. However, the driving force that controls the occurrence of OA is still poorly understood. Here, using in-situ liquid cell transmission electron microscopy, we demonstrate the ligand-controlled OA of citrate-stabilized gold nanoparticles at atomic resolution. Our data reveal that particle pairs rotate randomly at a separation distance greater than twice the layer thickness of adsorbed ligands...
January 29, 2018: Nature Communications
Giada Zurlo, Jérôme Piquereau, Maryline Moulin, Julie Pires Da Silva, Mélanie Gressette, Benoît Ranchoux, Anne Garnier, Renée Ventura-Clapier, Elie Fadel, Marc Humbert, Christophe Lemaire, Frédéric Perros, Vladimir Veksler
OBJECTIVE: Energy metabolism shift from oxidative phosphorylation toward glycolysis in pulmonary artery smooth muscle cells (PASMCs) is suggested to be involved in their hyperproliferation in pulmonary arterial hypertension (PAH). Here, we studied the role of the deacetylase sirtuin1 (SIRT1) in energy metabolism regulation in PASMCs via various pathways including activation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), master regulator of mitochondrial biogenesis...
January 24, 2018: Journal of Hypertension
Juan Jin, Taotao Liu, Mingxi Li, Chuxiao Yuan, Yang Liu, Jian Tang, Zhenqiang Feng, Yue Zhou, Fang Yang, Ning Gu
Inspired by the nature, the biomimetic nanomaterial design strategies have attracted great interest because the bioinspired nanoplatforms may enhance the functionality of current nanoparticles. Especially, the cell membrane-derived nanoparticles can more effectively navigate and interact with the complex biological microenvironment. In this study, we have explored a novel strategy to rapidly in situ biosynthesize gold nanoparticles (GNPs) in living platelets with the help of ultrasound energy. Firstly, under the ultrasound exposure, the biocompatible chloroauric acid salts (HAuCl4) can be enhanced to permeate into the platelet cytoplasm...
January 10, 2018: Colloids and Surfaces. B, Biointerfaces
Damien Roussel, Mélanie Boël, Caroline Romestaing
During food deprivation, animals must develop physiological responses to maximize energy conservation and survival. At the subcellular level, energy conservation is mainly achieved by a reduction in mitochondrial activity and an upregulation of oxidative phosphorylation efficiency. The aim of this study was to decipher mechanisms underlying the increased mitochondrial coupling efficiency reported in fasted birds. Mitochondrial oxidative phosphorylation activity, efficiency and membrane potential were measured in mitochondria isolated from gastrocnemius muscle of ducklings...
December 21, 2017: Journal of Experimental Biology
Xingxia Geng, Jiali Ye, Xuetong Yang, Sha Li, Lingli Zhang, Xiyue Song
Cytoplasmic male sterility (CMS) where no functional pollen is produced has important roles in wheat breeding. The anther is a unique organ for male gametogenesis and its abnormal development can cause male sterility. However, the mechanisms and regulatory networks related to plant male sterility are poorly understood. In this study, we conducted comparative analyses using isobaric tags for relative and absolute quantification (iTRAQ) of the pollen proteins in a CMS line and its wheat maintainer. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology classifications, metabolic pathways and transcriptional regulation networks using Blast2GO...
January 23, 2018: International Journal of Molecular Sciences
Rajaboopathi Mani, Huaidong Jiang, Santosh Kumar Gupta, Ziqing Li, Xiulan Duan
The europium ion probes the symmetry disorder in the crystal structure, although the distortion due to charge compensation in the case of aliovalent dopant remains interesting, especially preparation involves low and high temperatures. This work studies the preparation of the β-Ca2SiO4 (from here on C2S) particle from Pechini (C2SP) and hydrothermal (C2SH) methods, and its luminescence variance upon doping with Eu2+ and Eu3+ ions. The blue shift of the charge-transfer band (CTB) in the excitation spectra indicates a larger Eu3+-O2- distance in Eu3+ doped C2SH...
January 22, 2018: Inorganic Chemistry
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