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citrate energy

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https://www.readbyqxmd.com/read/29338257/a-dynamic-protein-protein-coupling-between-the-tonb-dependent-transporter-fhua-and-tonb
#1
Jessica Sarver, Michael Zhang, Lishan Liu, David Nyenhuis, David S Cafiso
Bacterial outer membrane TonB-dependent transporters function by executing cycles of binding and unbinding to the inner membrane protein TonB. In the vitamin B12 transporter BtuB and the ferric citrate transporter FecA, substrate binding increases the periplasmic exposure of the Ton box, an energy-coupling segment. This increased exposure appears to enhance the affinity of the transporter for TonB. Here, continuous wave and pulse EPR spectroscopy were used to examine the state of the Ton box in the Escherichia coli ferrichrome transporter, FhuA...
January 17, 2018: Biochemistry
https://www.readbyqxmd.com/read/29315839/citrate-improves-collagen-mineralization-via-interface-wetting-a-physicochemical-understanding-of-biomineralization-control
#2
Changyu Shao, Ruibo Zhao, Shuqin Jiang, Shasha Yao, Zhifang Wu, Biao Jin, Yuling Yang, Haihua Pan, Ruikang Tang
Biological hard tissues such as bones always contain extremely high levels of citrate, which is believed to play an important role in bone formation as well as in osteoporosis treatments. However, its mechanism on biomineralization is not elucidated. Here, it is found that the adsorbed citrate molecules on collagen fibrils can significantly reduce the interfacial energy between the biological matrix and the amorphous calcium phosphate precursor to enhance their wetting effect at the early biomineralization stage, sequentially facilitating the intrafibrillar formation of hydroxyapatite to produce an inorganic-organic composite...
January 8, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29312565/dissect-new-mechanistic-insights-for-geniposide-efficacy-on-the-hepatoprotection-using-multiomics-approach
#3
Shi Qiu, Aihua Zhang, Tianlei Zhang, Hui Sun, Yu Guan, Guangli Yan, Xijun Wang
A multi-omics approach could yield in-depth mechanistic insights. Here, we performed an integrated analysis of miRNAome, proteome and metabolome, aimed to investigate the underlying mechanism of active product geniposide in ethanol-induced apoptosis. We found that integrative meta-analysis identified 28 miRNAs, 20 proteins and 7 metabolites significantly differentially expressed, respectively. Further analysis identified geniposide extensively regulated multiple metabolism pathways and the most important related pathway was citrate cycle (TCA cycle)...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29303599/proteomic-analysis-of-peroxynitrite-induced-protein-nitration-in-isolated-beef-heart-mitochondria
#4
M Kohutiar, A Eckhardt, I Mikšík, P Šantorová, J Wilhelm
Mitochondria are exposed to reactive nitrogen species under physiological conditions and even more under several pathologic states. In order to reveal the mechanism of these processes we studied the effects of peroxynitrite on isolated beef heart mitochondria in vitro. Peroxynitrite has the potential to nitrate protein tyrosine moieties, break the peptide bond, and eventually release the membrane proteins into the solution. All these effects were found in our experiments. Mitochondrial proteins were resolved by 2D electrophoresis and the protein nitration was detected by immunochemical methods and by nano LC-MS/MS...
January 5, 2018: Physiological Research
https://www.readbyqxmd.com/read/29299709/fabrication-characterization-and-cytotoxicity-of-spherical-shaped-conjugated-gold-cockle-shell-derived-calcium-carbonate-nanoparticles-for-biomedical-applications
#5
Hanan Karimah Kiranda, Rozi Mahmud, Danmaigoro Abubakar, Zuki Abubakar Zakaria
The evolution of nanomaterial in science has brought about a growing increase in nanotechnology, biomedicine, and engineering fields. This study was aimed at fabrication and characterization of conjugated gold-cockle shell-derived calcium carbonate nanoparticles (Au-CSCaCO3NPs) for biomedical application. The synthetic technique employed used gold nanoparticle citrate reduction method and a simple precipitation method coupled with mechanical use of a Programmable roller-ball mill. The synthesized conjugated nanomaterial was characterized for its physicochemical properties using transmission electron microscope (TEM), field emission scanning electron microscope (FESEM) equipped with energy dispersive X-ray (EDX) and Fourier transform infrared spectroscopy (FTIR)...
January 2, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29289326/use-of-electrothermal-atomic-absorption-spectrometry-for-size-profiling-of-gold-and-silver-nanoparticles
#6
Teerawat Panyabut, Natnicha Sirirat, Atitaya Siripinyanond
Electrothermal atomic absorption spectrometry (ETAAS) was applied to investigate the atomization behaviors of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) in order to relate with particle size information. At various atomization temperatures from 1400 °C to 2200 °C, the time-dependent atomic absorption peak profiles of AuNPs and AgNPs with varying sizes from 5 nm to 100 nm were examined. With increasing particle size, the maximum absorbance was observed at the longer time. The time at maximum absorbance was found to linearly increase with increasing particle size, suggesting that ETAAS can be applied to provide the size information of nanoparticles...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29288934/microplastics-have-a-more-profound-impact-than-elevated-temperatures-on-the-predatory-performance-digestion-and-energy-metabolism-of-an-amazonian-cichlid
#7
Bin Wen, Nan Zhang, Shi-Rong Jin, Zai-Zhong Chen, Jian-Zhong Gao, Ying Liu, Han-Peng Liu, Zhe Xu
Knowledge on the impacts of microplastics (MPs) pollution on freshwater environments and biota remains limited. Meanwhile, freshwater ecosystems have been threatened by elevated temperatures caused by climate change. To date, no information exists on how MPs-especially under elevated temperature conditions-affect predatory performance, digestive processes and metabolic pathways in freshwater organisms. Here, we examined MPs, elevated temperature and their combined effects on juveniles (0+ group) of an Amazonian cichlid, the discus fish (Symphysodon aequifasciatus)...
December 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/29284039/from-in-vivo-to-in-vitro-major-metabolic-alterations-take-place-in-hepatocytes-during-and-following-isolation
#8
Shamir Cassim, Valérie-Ann Raymond, Pascal Lapierre, Marc Bilodeau
The liver plays a key role in maintaining physiological homeostasis and hepatocytes are largely responsible for this. The use of isolated primary hepatocytes has become an essential tool for the study of nutrient physiology, xenobiotic metabolism and several liver pathologies. Since hepatocytes are removed from their normal environment, the isolation procedure and in vitro culture of primary hepatocytes is partially known to induce undesired metabolic changes. We aimed to perform a thorough metabolic profiling of primary cells before, during and after isolation using state-of-the-art techniques...
2017: PloS One
https://www.readbyqxmd.com/read/29272915/shape-dependent-dissolution-and-cellular-uptake-of-silver-nanoparticles
#9
Christina Maria Graf, Daniel Nordmeyer, Christina Sengstock, Sebastian Ahlberg, Joerg Diendorf, Jörg Raabe, Matthias Epple, Manfred Köller, Juergen Lademann, Annika Vogt, Fiorenza Rancan, Eckart Ruehl
Cellular uptake and dissolution of trigonal silver nanoprisms (edge length: 42±15 nm, thickness: 8±1 nm) and mostly spherical silver nanoparticles (diameter: 70±25 nm) in human mesenchymal stem cells (hMSC) and human keratinocytes (HaCaT cells) were investigated. Both particles are stabilized by polyvinylpyrrolidone (PVP), the prisms additionally by citrate. The nanoprisms dissolved slightly in pure water but strongly in isotonic saline or at pH 4, corresponding to the lowest limit for the pH during cellular uptake...
December 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29258585/effects-of-endurance-training-on-skeletal-muscle-mitochondrial-function-in-huntington-disease-patients
#10
Sandro Manuel Mueller, Saskia Maria Gehrig, Jens A Petersen, Sebastian Frese, Violeta Mihaylova, Maria Ligon-Auer, Natalia Khmara, Jean-Marc Nuoffer, André Schaller, Carsten Lundby, Marco Toigo, Hans H Jung
BACKGROUND: Mitochondrial dysfunction may represent a pathogenic factor in Huntington disease (HD). Physical exercise leads to enhanced mitochondrial function in healthy participants. However, data on effects of physical exercise on HD skeletal muscle remains scarce. We aimed at investigating adaptations of the skeletal muscle mitochondria to endurance training in HD patients. METHODS: Thirteen HD patients and 11 healthy controls completed 26 weeks of endurance training...
December 19, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/29238173/a-light-assisted-in-situ-embedment-of-silver-nanoparticles-to-prepare-functionalized-fabrics
#11
Her Shuang Toh, Roxanne Line Faure, Liyana Bte Mohd Amin, Crystal Yu Fang Hay, Saji George
This article presents a simple, one-step, in situ generation of silver nanoparticle-functionalized fabrics with antibacterial properties, circumventing the conventional, multistep, time-consuming methods. Silver nanoparticle formation was studied with a library of capping agents (branched polyethylenimine [BPEI] of molecular weight [Mw] 10,000 and 25,000, polyvinylpyrrolidone, polyethylene glycol, polyvinylalcohol and citrate) mixed with silver nitrate. The mixture was then exposed to an assortment of light wavelengths (ultraviolet, infrared and simulated solar light) for studying the light-assisted synthesis of nanoparticles...
2017: Nanotechnology, Science and Applications
https://www.readbyqxmd.com/read/29230346/solid-state-kinetic-investigations-of-nonisothermal-reduction-of-iron-species-supported-on-sba-15
#12
N S Genz, D Baabe, T Ressler
Iron oxide catalysts supported on nanostructured silica SBA-15 were synthesized with various iron loadings using two different precursors. Structural characterization of the as-prepared Fe x O y /SBA-15 samples was performed by nitrogen physisorption, X-ray diffraction, DR-UV-Vis spectroscopy, and Mössbauer spectroscopy. An increasing size of the resulting iron species correlated with an increasing iron loading. Significantly smaller iron species were obtained from (Fe(III), NH4)-citrate precursors compared to Fe(III)-nitrate precursors...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/29226743/molecular-docking-and-molecular-dynamics-simulations-of-fumarate-hydratase-and-its-mutant-h235n-complexed-with-pyromellitic-acid-and-citrate
#13
S Subasri, Santosh Kumar Chaudhary, K Sekar, Manish Kesherwani, D Velmurugan
Fumarase catalyzes the reversible, stereospecific hydration/dehydration of fumarate to L-malate during the Kreb's cycle. In the crystal structure of the tetrameric fumarase, it was found that some of the active site residues S145, T147, N188 G364 and H235 had water-mediated hydrogen bonding interactions with pyromellitic acid and citrate which help to the protonation state for the conversion of fumarate to malate. When His 235 is mutated with Asn (H235N), water-mediated interactions were lost due to the shifting of active site water molecule by 0...
December 2017: Journal of Bioinformatics and Computational Biology
https://www.readbyqxmd.com/read/29213186/cyclic-fractionation-process-for-saccharina-latissima-using-aqueous-chelator-and-ion-exchange-resin
#14
Martin Sterner, Mauricio Sodré Ribeiro, Fredrik Gröndahl, Ulrica Edlund
A new approach to process Saccharina latissima algal biomass was developed using sodium citrate and a polyvalent cation-specific resin to sequentially extract the alginate into several usable fractions. The fractionation was performed in a cyclic manner, utilizing a stepwise removal of the native polyvalent ions present in the algae to isolate fractions of alginate with different solubility in the presence of these ions. Sodium citrate was used in different concentrations in the extraction solution to remove polyvalent cations to adjust the alginate liberation while AMBERLITE IRC718 resin was added to further remove these ions and regenerate the extraction solution...
2017: Journal of Applied Phycology
https://www.readbyqxmd.com/read/29212325/chronic-heat-stress-impairs-meat-quality-of-breast-muscle-in-broilers-through-affecting-redox-status-and-energy-substances-metabolism
#15
Zhuang Lu, Xiaofang He, Bingbing Ma, Lin Zhang, Jiaolong Li, Yun Jiang, Guanghong Zhou, Feng Gao
We investigated the molecular mechanisms of chronic heat stress impairing broilers breast meat quality. Broilers were assigned to three groups including normal control (NC) group, heat stress (HS) group and pair-fed (PF) group. After 7d of heat exposure (32°C), high temperature caused oxidative stress, and elevated the activity of citrate synthase (CS), mRNA expression of M-CPT1, and phosphorylation level of AMPKα, and reduced the mRNA expression of avUCP. After 14d of heat exposure, heat stress increased lightness and drip loss and decreased pH and shear force of breast meat...
December 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29211964/metabolic-costs-of-exposure-to-wastewater-effluent-lead-to-compensatory-adjustments-in-respiratory-physiology-in-bluegill-sunfish
#16
Sherry Du, Erin S McCallum, Maryam Vaseghi-Shanjani, Jasmine A Choi, Theresa R Warriner, Sigal Balshine, Graham R Scott
Municipal wastewater effluent is a major source of aquatic pollution and has potential to impact cellular energy metabolism. However, it is poorly understood whether wastewater exposure impacts whole-animal metabolism and whether this can be accommodated with adjustments in respiratory physiology. We caged bluegill sunfish (Lepomis macrochirus) for 21 days at two sites downstream (either 50 m or 830 m) from a wastewater treatment plant (WWTP). Survival was reduced in fish caged at both downstream sites compared to an uncontaminated reference site...
December 6, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29159707/propionyl-coa-carboxylase-pcca-1-and-pccb-1-gene-deletions-in-caenorhabditis-elegans-globally-impair-mitochondrial-energy-metabolism
#17
Kimberly A Chapman, Julian Ostrovsky, Meera Rao, Stephen D Dingley, Erzsebet Polyak, Marc Yudkoff, Rui Xiao, Michael J Bennett, Marni J Falk
Propionic acidemia (PA) is a classical inborn error of metabolism with high morbidity that results from the inability of the propionyl-CoA carboxylase (PCC) enzyme to convert propionyl-CoA to methylmalonyl-CoA. PA is inherited in an autosomal recessive fashion due to functional loss of both alleles of either PCCA or PCCB. These genes are highly conserved across evolutionarily diverse species and share extensive similarity with pcca-1 and pccb-1 in the nematode, Caenorhabditis elegans. Here, we report the global metabolic effects of deletion in a single PCC gene, either pcca-1 or pccb-1, in C...
November 20, 2017: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/29152896/trimetazidine-restores-the-positive-adaptation-to-exercise-training-by-mitigating-statin-induced-skeletal-muscle-injury
#18
Ming Song, Fang-Fang Chen, Yi-Hui Li, Lei Zhang, Feng Wang, Ran-Ran Qin, Zhi-Hao Wang, Ming Zhong, Meng-Xiong Tang, Wei Zhang, Lu Han
BACKGROUND: Exercise rehabilitation is demonstrated to improve the prognosis of patients with coronary heart disease (CHD). Statins, as the key medicine to lower cholesterol in CHD, result in skeletal muscle injury and impair exercise training adaptation. Energy metabolism dysfunction is identified as the potential mechanism underlying statin-induced skeletal muscle injury. In this study, we investigated the effects of the metabolic modulator trimetazidine on skeletal muscle energy metabolism and statin-associated exercise intolerance...
November 19, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29151592/diet-resistant-obesity-is-characterized-by-a-distinct-plasma-proteomic-signature-and-impaired-muscle-fiber-metabolism
#19
A B Thrush, G Antoun, M Nikpay, D A Patten, C DeVlugt, J-F Mauger, B L Beauchamp, P Lau, R Reshke, É Doucet, P Imbeault, R Boushel, D Gibbings, J Hager, A Valsesia, R Slack, O Y Al-Dirbashi, R Dent, R McPherson, M-E Harper
BACKGROUND/OBJECT: Inter-individual variability in weight loss during obesity treatment is complex and poorly understood. Here we use whole body and tissue approaches to investigate fuel oxidation characteristics in skeletal muscle fibers, cells, and distinct circulating protein biomarkers before and after a high fat meal (HFM) challenge in those who lost the most (obese diet-sensitive; ODS), vs the least (obese diet-resistant; ODR) amount of weight in a highly controlled weight management program...
November 20, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29137472/changes-in-transcription-pattern-lead-to-a-marked-decrease-in-cox-cs-and-sqr-activity-after-the-developmental-point-of-the-22-nd-gestational-week
#20
H Kolarova, J Krizova, M Hulkova, H Hansikova, H Hulkova, V Smid, J Zeman, T Honzik, M Tesarova
Tissue differentiation and proliferation throughout fetal development interconnect with changes in the Oxidative Phosphorylation System (OXPHOS) on the cellular level. Reevaluation of the expression data revealed a significant increase in COX4 and MTATP6 liver transcription levels after the 22(nd) gestational week (GW) which inspired us to characterize its functional impact. Specific activities of cytochrome c oxidase (COX), citrate synthase (CS), succinate-coenzyme Q reductase (SQR) and mtDNA determined by spectrophotometry and RT-PCR were studied in a set of 25 liver and 18 skeletal muscle samples at 13(th) to 29(th) GW...
November 10, 2017: Physiological Research
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