Ning Yuan Lee, Melissa Hum, Guek Peng Tan, Ai Choo Seah, Patricia T Kin, Ngiap Chuan Tan, Hai-Yang Law, Ann S G Lee
BACKGROUND: Blood-based DNA methylation has shown great promise as a biomarker in a wide variety of diseases. Studies of DNA methylation in blood often utilize samples which have been cryopreserved for years or even decades. Therefore, changes in DNA methylation associated with long-term cryopreservation can introduce biases or otherwise mislead methylation analyses of cryopreserved DNA. However, previous studies have presented conflicting results with studies reporting hypomethylation, no effect, or even hypermethylation of DNA following long-term cryopreservation...
September 11, 2023: Clinical Epigenetics