keyword
MENU ▼
Read by QxMD icon Read
search

in vivo imaging

keyword
https://www.readbyqxmd.com/read/29046056/overview-of-the-optical-properties-of-fluorescent-nanoparticles-for-optical-imaging
#1
Federico Boschi, Francesco De Sanctis
Nanoparticles (NPs) include a wide group of small structures composed by very different materials and characterized by peculiar properties that make them suitable for many applications, especially imaging and drug delivery. In this overview we focus on the optical properties of fluorescent NPs available for in vivo, in vitro and ex vivo preclinical studies and detectable with the optical imaging technique alone or in combination with microscopic confocal imaging. We summarize here the basic principles of the optical detection of fluorescent NPs, elucidate which are the current issues to be resolved and possible solutions to achieve the highest sensitivity and specificity for an unbiased analysis...
August 29, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29045826/a-cell-migration-tracking-tool-supports-coupling-of-tissue-rotation-to-elongation
#2
Dong-Yuan Chen, Justin Crest, David Bilder
Cell migration is indispensable to morphogenesis and homeostasis. Live imaging allows mechanistic insights, but long-term observation can alter normal biology, and tools to track movements in vivo without perturbation are lacking. We develop here a tool called M-TRAIL (matrix-labeling technique for real-time and inferred location), which reveals migration histories in fixed tissues. Using clones that overexpress GFP-tagged extracellular matrix (ECM) components, motility trajectories are mapped based on durable traces deposited onto basement membrane...
October 17, 2017: Cell Reports
https://www.readbyqxmd.com/read/29045825/cerenkov-luminescence-imaging-on-evaluation-of-early-response-to-chemotherapy-of-drug-resistant-gastric-cancer
#3
Muhan Liu, Sheng Zheng, Xiaojun Zhang, Hongbo Guo, Xiaojing Shi, Xiaoyu Kang, Yawei Qu, Zhenhua Hu, Jie Tian
Apoptosis imaging enables a timely and specific assessment of treatment response in cancer patients. In this study, we applied a probe for positron emission tomography (PET), served as an optical biomaterial emitting Cerenkov photons, to in vivo optical imaging of tumor apoptosis, in order to evaluate early response to chemotherapy of drug-resistant gastric cancer. (68)Ga-DOTA-Annexin V was prepared as the apoptosis targeting probe. Wild type human gastric adenocarcinoma cell line SGC7901/WT and drug vincristine-resistant variant SGC7901/VCR were used to establish normal and vincristine-resistant xenograft to simulate treatment decision situation...
October 15, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29045067/reconnectable-fiberscopes-for-chronic-in-vivo-deep-brain-imaging
#4
M S Pochechuev, I V Fedotov, O I Ivashkina, M A Roshchina, D V Meshchankin, D A Sidorov-Biryukov, A B Fedotov, K V Anokhin, A M Zheltikov
Reconnectable bundles consisting of thousands of optical fibers are shown to enable high-quality image transmission, offering a platform for the creation of implantable fiberscopes for minimally invasive in vivo brain imaging. Experiments on various lines of transgenic mice verify the performance of this fiberscope as a powerful tool for chronic in vivo neuroimaging using genetically encoded calcium indicators, neuronal activity markers, as well as axon growth regulators and brain-specific protein drivers in deep regions of live brain...
October 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29044888/nuclear-magnetic-relaxation-dispersion-of-murine-tissue-for-development-of-t1-r1-dispersion-contrast-imaging
#5
Yonathan T Araya, Francisco Martínez-Santiesteban, William B Handler, Chad T Harris, Blaine A Chronik, Timothy J Scholl
This study quantified the spin-lattice relaxation rate (R1 ) dispersion of murine tissues from 0.24 mT to 3 T. A combination of ex vivo and in vivo spin-lattice relaxation rate measurements were acquired for murine tissue. Selected brain, liver, kidney, muscle, and fat tissues were excised and R1 dispersion profiles were acquired from 0.24 mT to 1.0 T at 37 °C, using a fast field-cycling MR (FFC-MR) relaxometer. In vivo R1 dispersion profiles of mice were acquired from 1.26 T to 1.74 T at 37 °C, using FFC-MRI on a 1...
October 18, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/29044762/density-weighted-concentric-rings-k-space-trajectory-for-1-h-magnetic-resonance-spectroscopic-imaging-at-7%C3%A2-t
#6
Mark Chiew, Wenwen Jiang, Brian Burns, Peder Larson, Adam Steel, Peter Jezzard, M Albert Thomas, Uzay E Emir
It has been shown that density-weighted (DW) k-space sampling with spiral and conventional phase encoding trajectories reduces spatial side lobes in magnetic resonance spectroscopic imaging (MRSI). In this study, we propose a new concentric ring trajectory (CRT) for DW-MRSI that samples k-space with a density that is proportional to a spatial, isotropic Hanning window. The properties of two different DW-CRTs were compared against a radially equidistant (RE) CRT and an echo-planar spectroscopic imaging (EPSI) trajectory in simulations, phantoms and in vivo experiments...
October 18, 2017: NMR in Biomedicine
https://www.readbyqxmd.com/read/29044721/body-diffusion-weighted-imaging-using-magnetization-prepared-single-shot-fast-spin-echo-and-extended-parallel-imaging-signal-averaging
#7
Eric K Gibbons, Shreyas S Vasanawala, John M Pauly, Adam B Kerr
PURPOSE: This work demonstrates a magnetization prepared diffusion-weighted single-shot fast spin echo (SS-FSE) pulse sequence for the application of body imaging to improve robustness to geometric distortion. This work also proposes a scan averaging technique that is superior to magnitude averaging and is not subject to artifacts due to object phase. THEORY AND METHODS: This single-shot sequence is robust against violation of the Carr-Purcell-Meiboom-Gill (CPMG) condition...
October 17, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29044710/tracking-the-progression-of-osteolytic-and-osteosclerotic-lesions-in-mice-using-serial-in-vivo-micro-ct-applications-to-the-assessment-of-bisphosphonate-treatment-efficacy
#8
G M Campbell, R J Tower, T Damm, P Kneissl, A Rambow, C Schem, S Tiwari, C C Glüer
The metastasis of tumour cells to bone can lead to osteolytic and osteosclerotic lesions, which cause severe, highly-localized bone destruction and abnormal bone apposition, respectively. Accurate quantification of lesion progression is critical to understand underlying mechanisms and assess treatment efficacy; however, standard structural parameters may be insensitive to local changes. We developed methods to quantify osteolytic and osteosclerotic lesions using micro-computed tomography (micro-CT) within in vivo mouse datasets...
October 18, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29044537/in-vivo-imaging-of-the-internal-nasal-valve-during-different-conditions-using-optical-coherence-tomography
#9
Anna S Englhard, Maximilian Wiedmann, Georg J Ledderose, Bryan Lemieux, Alan Badran, Joseph C Jing, Zhongping Chen, Veronika Volgger, Brian J F Wong
OBJECTIVE: Previously, we proposed long-range optical coherence tomography (LR-OCT) to be an effective method for the quantitative evaluation of the nasal valve geometry. Here, the objective was to quantify the reduction in the internal nasal valve angle and cross-sectional area that results in subjective nasal airway obstruction and to evaluate the dynamic behavior of the valve during respiration using LR-OCT. METHODS: For 16 healthy individuals, LR-OCT was performed in each naris during: 1) normal respiration, 2) peak forced inspiration, 3) lateral nasal wall depression (to the onset of obstructive symptoms), and 4) after application of a topical decongestant...
October 17, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29044327/generation-of-a-triple-fluorescent-mouse-strain-allows-a-dynamic-and-spatial-visualization-of-different-liver-phagocytes-in-vivo
#10
Brenda N Nakagaki, Maria A Freitas-Lopes, Érika Carvalho, Raquel Carvalho-Gontijo, Hortência M Castro-Oliveira, Rafael M Rezende, Denise C Cara, Mônica M Santos, Rodrigo Pestana Lopes, Bruna A David, Gustavo B Menezes
Resident and circulating immune cells have been extensively studied due to their almost ubiquitous role in cell biology. Despite their classification under the "immune cell department", it is becoming increasingly clear that these cells are involved in many different non-immune related phenomena, including fetus development, vascular formation, memory, social behavior and many other phenotypes. There is a huge potential in combining high-throughput assays - including flow cytometry and gene analysis - with in vivo imaging...
October 16, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29043714/spectroscopic-doppler-analysis-for-visible-light-optical-coherence-tomography
#11
Xiao Shu, Wenzhong Liu, Lian Duan, Hao F Zhang
Retinal oxygen metabolic rate can be effectively measured by visible-light optical coherence tomography (vis-OCT), which simultaneously quantifies oxygen saturation and blood flow rate in retinal vessels through spectroscopic analysis and Doppler measurement, respectively. Doppler OCT relates phase variation between sequential A-lines to the axial flow velocity of the scattering medium. The detectable phase shift is between -π and π due to its periodicity, which limits the maximum measurable unambiguous velocity without phase unwrapping...
October 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29043675/2in1-vectors-improve-in-planta-bifc-and-fret-analyses
#12
Dietmar G Mehlhorn, Niklas Wallmeroth, Kenneth W Berendzen, Christopher Grefen
Protein-protein interactions (PPIs) play vital roles in all subcellular processes and a number of tools have been developed for their detection and analysis. Each method has its unique set of benefits and drawbacks that need to be considered prior to their application. In fact, researchers are spoilt for choice when it comes to deciding which method to use for the initial detection of a PPI, and which to corroborate the findings. With constant improvements in microscope development, the possibilities of techniques to study PPIs in vivo, and in real time, are continuously enhanced, and expanded...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29043471/nanostructured-lipid-carrier-co-loaded-with-doxorubicin-and-docosahexaenoic-acid-as-a-theranostic-agent-evaluation-of-biodistribution-and-antitumor-activity-in-experimental-model
#13
Renata S Fernandes, Juliana O Silva, Samuel V Mussi, Sávia C A Lopes, Elaine A Leite, Geovanni D Cassali, Valbert N Cardoso, Danyelle M Townsend, Patrick M Colletti, Lucas A M Ferreira, Domenico Rubello, André L B de Barros
PURPOSE: Nanotheranostic platforms, i.e., the combination of both therapeutic and diagnostic agents on a single platform, are emerging as an interesting tool for the personalized cancer medicine. Therefore, the aim of this work was to evaluate the in vivo properties of a Tc-99m-labeled nanostructured lipid carrier (NLC) formulation, co-loaded with doxorubicin (DOX) and docosahexaenoic acid (DHA), for theranostic applications. PROCEDURES: NLC-DHA-DOX were prepared busing the hot melting homogenization method using an emulsification-ultrasound and were radiolabeled with Tc-99m...
October 17, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29043366/distance-dependent-photoacoustics-revealed-through-dna-nanostructures
#14
James Joseph, Kevin N Baumann, Philipp Koehler, Tim J Zuehlsdorff, Daniel J Cole, Judith Weber, Sarah E Bohndiek, Silvia Hernández-Ainsa
Molecular rulers that rely on the Förster resonance energy transfer (FRET) mechanism are widely used to investigate dynamic molecular processes that occur on the nanometer scale. However, the capabilities of these fluorescence molecular rulers are fundamentally limited to shallow imaging depths by light scattering in biological samples. Photoacoustic tomography (PAT) has recently emerged as a high resolution modality for in vivo imaging, coupling optical excitation with ultrasound detection. In this paper, we report the capability of PAT to probe distance-dependent FRET at centimeter depths...
October 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/29043083/a-framework-for-continuous-target-tracking-during-mr-guided-high-intensity-focused-ultrasound-thermal-ablations-in-the-abdomen
#15
Cornel Zachiu, Baudouin Denis de Senneville, Ivan D Dmitriev, Chrit T W Moonen, Mario Ries
BACKGROUND: During lengthy magnetic resonance-guided high intensity focused ultrasound (MRg-HIFU) thermal ablations in abdominal organs, the therapeutic work-flow is frequently hampered by various types of physiological motion occurring at different time-scales. If left un-addressed this can lead to an incomplete therapy and/or to tissue damage of organs-at-risk. While previous studies focus on correction schemes for displacements occurring at a particular time-scale within the work-flow of an MRg-HIFU therapy, in the current work we propose a motion correction strategy encompassing the entire work-flow...
2017: Journal of Therapeutic Ultrasound
https://www.readbyqxmd.com/read/29042775/mesoporous-composite-nanoparticles-for-dual-modality-ultrasound-magnetic-resonance-imaging-and-synergistic-chemo-thermotherapy-against-deep-tumors
#16
Nan Zhang, Ronghui Wang, Junnian Hao, Yang Yang, Hongmi Zou, Zhigang Wang
High-intensity focused ultrasound (HIFU) is a promising and noninvasive treatment for solid tumors, which has been explored for potential clinical applications. However, the clinical applications of HIFU for large and deep tumors such as hepatocellular carcinoma (HCC) are severely limited by unsatisfactory imaging guidance, long therapeutic times, and damage to normal tissue around the tumor due to the high power applied. In this study, we developed doxorubicin/perfluorohexane-encapsulated hollow mesoporous Prussian blue nanoparticles (HMPBs-DOX/PFH) as theranostic agents, which can effectively guide HIFU therapy and enhance its therapeutic effects in combination with chemotherapy, by decreasing the cavitation threshold...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29042770/bsa-assisted-synthesis-of-ultrasmall-gallic-acid-fe-iii-coordination-polymer-nanoparticles-for-cancer-theranostics
#17
Xueling Mu, Chenglin Yan, Qiwei Tian, Jiaomin Lin, Shiping Yang
Protein-related nanotheranostic agents hold great promise as tools to serve many clinical applications. Proteins such as BSA are used to regulate the synthesis of nondegradable inorganic nanoparticles (NPs). To fully employ the potential of such proteins, a new type of biosafe nanotheranostic agent must be designed to optimize BSA as a biomineralization agent. Here, a straightforward BSA-assisted biomineralization method was developed to prepare gallic acid (GA)-Fe(III) coordination polymer NPs. BSA-coated GA-Fe (GA-Fe@BSA) NPs were ultrasmall (3...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29042701/direct-damage-to-a-vertebral-artery-better-predicts-a-vertebral-artery-injury-than-elongation-in-cervical-spine-dislocation
#18
Kosei Nagata, Hirotaka Chikuda, Koichi Inokuchi, Keisuke Ishii, Atsuki Kobayashi, Hiroyuki Kanai, Kota Miyoshi
Cervical spine dislocation and fracture of a transverse process are isolated risk factors for vertebral artery injuries (VAIs), which can cause a life-threatening ischemic stroke. Since in vivo experiments are not possible, it has not been unclear whether damage to or extension of vertebral arteries is more predictive of a VAI. To identify the imaging characteristics associated with VAI, we analyzed 36 vertebral arteries from 22 cervical spine dislocation patients who underwent computed tomography angiography (Aug...
October 2017: Acta Medica Okayama
https://www.readbyqxmd.com/read/29042650/handheld-photoacoustic-microscopy-probe
#19
Kyungjin Park, Jin Young Kim, Changho Lee, Seungwan Jeon, Geunbae Lim, Chulhong Kim
Optical resolution photoacoustic microscopy (OR-PAM) is a non-invasive, label-free method of in vivo imaging with microscopic resolution and high optical contrast. Based on intrinsic contrasts, OR-PAM has expanded to include in vivo vessel imaging, flow cytometry, physiological parameter analysis, and single-cell characterization. However, since conventional OR-PAM systems have a fixed tabletop configuration, a large system size, and slow imaging speed, their use in preclinical and clinical studies remains limited...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042617/expression-and-function-of-abcg4-in-the-mouse-blood-brain-barrier-role-in-restricting-the-brain-entry-of-amyloid-%C3%AE-peptide
#20
Agnès Dodacki, Matthew Wortman, Bruno Saubaméa, Stéphanie Chasseigneaux, Sophie Nicolic, Nathalie Prince, Murielle Lochus, Anne-Laure Raveu, Xavier Declèves, Jean-Michel Scherrmann, Shailendra B Patel, Fanchon Bourasset
ABCG4 is an ATP-binding cassette transmembrane protein which has been shown, in vitro, to participate in the cellular efflux of desmosterol and amyloid-β peptide (Aβ). ABCG4 is highly expressed in the brain, but its localization and function at the blood-brain barrier (BBB) level remain unknown. We demonstrate by qRT-PCR and confocal imaging that mouse Abcg4 is expressed in the brain capillary endothelial cells. Modelling studies of the Abcg4 dimer suggested that desmosterol showed thermodynamically favorable binding at the putative sterol-binding site, and this was greater than for cholesterol...
October 17, 2017: Scientific Reports
keyword
keyword
38239
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"