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in vivo imaging

Xin Zhou, De Cai, Xiaolong He, Sung-Liang Chen, Xueding Wang, Tian Yang
Ultrasound detection is performed by measuring laser reflection off a surface plasmon resonance cavity which is integrated at a single-mode fiber end facet. It shows a total noise (or laser RIN) equivalent pressure of 9.7 KPa (or 5.2 KPa) over 0-20 MHz, a 6-dB angular detection range as large as 70° near 10 MHz, a detection bandwidth larger than 125 MHz, and a stable performance for over 20 min without feedback in ambient conditions. Its small form factor, fiber-optic integration, almost omnidirectional responsivity and large bandwidth are favorable for in vivo applications and high resolution imaging...
February 15, 2018: Optics Letters
Hui Gao, Xin Zhao, Sijie Chen
In recent years, luminogens with the feature of aggregation-induced emission (AIEgen) have emerged as advanced luminescent materials for fluorescent nanomaterial preparation. AIEgen-based nanomaterials show enhanced fluorescence efficiency and superior photostability, which thusly offer unique advantages in biological applications. In this review, we will summarize the fabrication methods of AIEgen-based nanomaterials and their applications in in vitro/in vivo imaging, cell tracing, photodynamic therapy and drug delivery, focusing on the recent progress...
February 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Sandra B Lemke, Frank Schnorrer
Muscles together with tendons and the skeleton enable animals including humans to move their body parts. Muscle morphogenesis is highly conserved from animals to humans. Therefore, the powerful Drosophila model system can be used to study concepts of muscle-tendon development that can also be applied to human muscle biology. Here, we describe in detail how morphogenesis of the adult muscle-tendon system can be easily imaged in living, developing Drosophila pupae. Hence, the method allows investigating proteins, cells and tissues in their physiological environment...
February 6, 2018: Journal of Visualized Experiments: JoVE
Sang A Park, Young Ho Choe, Sung Hwan Lee, Young-Min Hyun
Sepsis is a type of severe infection that can cause organ failure and tissue damage. Although the mortality and morbidity rates associated with sepsis are extremely high, no direct treatment or organ-related mechanism has been examined in detail in real time. The liver is the key organ that manages toxins and infections in the human body. Herein, we aimed to perform intravital imaging of mouse liver after induction of endotoxemia in order to track the motility of immune cells, such as neutrophils and liver capsular macrophages (LCMs)...
February 6, 2018: Journal of Visualized Experiments: JoVE
Kaela M Varberg, Seth Winfree, Kenneth W Dunn, Laura S Haneline
Vasculogenesis is a complex process by which endothelial stem and progenitor cells undergo de novo vessel formation. Quantitative assessment of vasculogenesis has become a central readout of endothelial progenitor cell functionality, and therefore, several attempts have been made to improve both in vitro and in vivo vasculogenesis models. However, standard methods are limited in scope, with static measurements failing to capture many aspects of this highly dynamic process. Therefore, the goal of developing this novel protocol was to assess the kinetics of in vitro vasculogenesis in order to quantitate rates of network formation and stabilization, as well as provide insight into potential mechanisms underlying vascular dysfunction...
January 31, 2018: Journal of Visualized Experiments: JoVE
Symantas Ragauskas, Eva Kielczewski, Joseph Vance, Simon Kaja, Giedrius Kalesnykas
Laser-induced choroidal neovascularization (CNV) is a well-established model to mimic the wet form of age-related macular degeneration (AMD). In this protocol, we aim to guide the reader not simply through the technical considerations of generating laser-induced lesions to trigger neovascular processes, but rather focus on the powerful information that can be obtained from multimodal longitudinal in vivo imaging throughout the follow-up period. The laser-induced mouse CNV model was generated by a diode laser administration...
January 21, 2018: Journal of Visualized Experiments: JoVE
Vivek Borse, Adisha Kashikar, Rohit Srivastava
Quantum dots are the semiconductor nanocrystals having unique optical and electronic properties. Quantum dots are category of fluorescent labels utilized for biological tagging, biosensing, bioassays, bioimaging and in vivo imaging as they exhibit very small size, signal brightness, photostability, tuning of light emission range, longer photoluminescence decay time as compared to organic dyes. In this work, we have synthesized and characterized mercaptopropionic acid capped cadmium telluride quantum dots (MPA-CdTe QDs) using hydrothermal method...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Zehong Yang, Hua Zhuang, Hong Song, Ji Liu, Xiaojun Zhao, Wei Lin
BACKGROUND: Despite of intensive efforts, the tumor progression of ovarian cancer has not yet been fully understood. Various studies suggest that an effective experimental model system In Vitro to mimic microenvironment in vivo and the fabrication of cell pattern formation In Vitro are of great importance for the ovarian cancer research. METHODS: This study cultivates SKOV3, A2780 and A2780/DDP ovarian cancer cell lines in RADA16-I peptide hydrogel to obtain 3D-precultured cancer cells and fabricate a type of miniature cell pattern formation In Vitro on RADA16-I peptide nanofibercoated coverslip by in situ AFM analysis and chemosensitivity assay in vitro...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Xiao-Ning Lin, Xinli Tian, Wang Li, Jin Sun, Feng Wei, Wei Feng, Zhi-Chun Huang, Xin-Hua Tian
Gliomas are the most common type of intracranial malignant tumor; however, current treatment approaches are often ineffective due to limited penetration of genes or drugs through the blood-brain barrier (BBB). Here we describe the synthesis of gelatin-siloxane nanoparticles (GS NPs) as candidate gene carriers through a two-step sol-gel process. To increase the efficiency of glioma targeting, human immunodeficiency virus-derived Tat, tumor-targeting aptamer (TTA)1, and polyethylene glycol (PEG) were conjugated to the GS NPs to generate Tat-TTA1-PEG-GS NPs...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Nguyen Thuy Chinh, Nguyen Thi Thu Trang, Tran Thi Mai, Dinh Thi Mai Thanh, Tran Huu Trung, Trinh Hoang Trung, Le Van Quan, Nguyen Thi Hoa, Can Van Mao, Nguyen Trong Nghia, Nguyen Thanh Hai, Tran Hong Anh, Tran Viet Hung, Jin-Ho Choy, Nguyen Van Loi, Baskaran Rajesh, Thai Hoang
This paper presents the results of zeta potential, water contact angle, atomic force microscopy image, in vitro solubility, and content of heavy metals in polylactic acid (PLA)/chitosan (CS) nanoparticles loading nifedipine. In addition, the In Vivo test of the PLA/CS nanoparticles loading nifedipine in the mice is also one of highlights of this work. The Zeta potential result shows that the charged surface of the PLA/CS nanoparticles loading nifedipine is neutral, negative or complex depending on nifedipine content...
April 1, 2018: Journal of Nanoscience and Nanotechnology
Arjan D Hendriks, Alessio Fracasso, Catalina S Arteaga de Castro, Mark W J M Gosselink, Peter R Luijten, Natalia Petridou, Dennis W J Klomp
The combination of functional MRI (fMRI) and MRS is a promising approach to relate BOLD imaging to neuronal metabolism, especially at high field strength. However, typical scan times for GABA edited spectroscopy are of the order of 6-30 min, which is long compared with functional changes observed with fMRI. The aim of this study is to reduce scan time and increase GABA sensitivity for edited spectroscopy in the human visual cortex, by enlarging the volume of activated tissue in the primary visual cortex. A dedicated setup at 7 T for combined fMRI and GABA MRS is developed...
February 14, 2018: NMR in Biomedicine
Christoph Rathgeb, Lukas Anschuetz, Daniel Schneider, Cilgia Dür, Marco Caversaccio, Stefan Weber, Tom Williamson
OBJECTIVE: We aimed to design, build and validate a surgical navigation system which fulfills the accuracy requirements for surgical procedures on the ear and the lateral skull base, and which integrates with the endoscopic workflow and operating room setup. MATERIALS AND METHODS: The navigation system consists of portable tablet computer (iPad Pro, Apple Computer, USA) and an optical tracking system (Cambar B1, Axios3D, Germany), both connected via a wireless Bluetooth link and attached directly to the OR table...
February 13, 2018: European Archives of Oto-rhino-laryngology
Yi-Wei Xie, Zhao-Yun Li, Jing Du, Yu Chen, Bing-Yu Chen, Tong-Tong Wang, Zhihui Huang, Shuangxing Hou, Ying Wang
Interneurons in the olfactory bulb (OB) are generated from neuronal precursor cells migrating from anterior subventricular zone (SVZa) not only in the developing embryo but also throughout the postnatal life of mammals. In the present study, we established an in vivo electroporation assay to label SVZa cells of rat both at embryonic and postnatal ages, and traced SVZa progenitors and followed their migration pathway and differentiation. We found that labeled cells displayed high motility. Interestingly, the postnatal cells migrated faster than the embryonic cells after applying this assay at different ages of brain development...
February 13, 2018: Cellular and Molecular Neurobiology
Xiao-Qian Dou, Hua Wang, Jing Zhang, Fang Wang, Gui-Li Xu, Cheng-Cheng Xu, Huan-Hua Xu, Shen-Si Xiang, Jie Fu, Hai-Feng Song
Introduction: The toxic side effects of doxorubicin (DOX) have limited its use in chemotherapy. Neither liposomal DOX nor pegylated liposomal DOX are able to completely resolve this issue. This is a proof-of-concept study testing aptamer-drug conjugate (ApDC) targeted delivery systems for chemotherapeutic drugs. Methods: Aptamer library targeting human epidermal growth factor receptor 3 (HER3) was screened and affinity was determined by enzyme-linked immunosorbent assay...
2018: International Journal of Nanomedicine
Qingqing Meng, Aiping Wang, Hongchen Hua, Ying Jiang, Yiyun Wang, Hongjie Mu, Zimei Wu, Kaoxiang Sun
Background: Safe and effective delivery of therapeutic drugs to the brain is important for successful therapy of Alzheimer's disease (AD). Purpose: To develop Huperzine A (HupA)-loaded, mucoadhesive and targeted polylactide-co-glycoside (PLGA) nanoparticles (NPs) with surface modification by lactoferrin (Lf)-conjugated N-trimethylated chitosan (TMC) (HupA Lf-TMC NPs) for efficient intranasal delivery of HupA to the brain for AD treatment. Methods: HupA Lf-TMC NPs were prepared using the emulsion-solvent evaporation method and optimized using the Box-Behnken design...
2018: International Journal of Nanomedicine
Melanie D White, Ziqing W Zhao, Nicolas Plachta
Live imaging has transformed biomedical sciences by enabling visualization and analysis of dynamic cellular processes as they occur in their native contexts. Here, we review key recent efforts applying in vivo optical imaging with single-cell resolution to mammalian systems ranging from embryos to adult tissues and organs. We highlight insights into active processes regulating cell fate and morphogenesis during embryonic development, how neuronal circuitry and non-neuronal cell types contribute to neurological functions, and how novel imaging-based approaches enable the dissection of neurological disorders and cancer with high spatio-temporal resolution...
February 10, 2018: Trends in Molecular Medicine
Yixia Qian, Weizhi Wang, Zihua Wang, Xiangqian Jia, Qiuju Han, Iman Rostami, Yuehua Wang, Zhiyuan Hu
Mild acidic environment and angiogenesis are two typical characteristics of tumor. The specific response towards both lower pH and angiogenesis may enhance the targeting ability both for drug and diagnostic probe delivery. Herein, we present a kind of dual responding self-assembled nanotransformation materials which are tumor angiogenesis targeting and pH triggered based on amphiphilic conjugation between peptide (STP) and aromatic molecule (TPE). The morphology of the self-assembled peptide-conjugates is responsibly changed from nanoparticles in neutral condition to nanofibers in acidic condition, which "turn on" the in vivo targeting imaging and accelerated the efficient drug delivery and in vivo therapy...
February 14, 2018: ACS Applied Materials & Interfaces
Gregor-Alexander Pilz, Sara Bottes, Marion Betizeau, David J Jörg, Stefano Carta, Benjamin D Simons, Fritjof Helmchen, Sebastian Jessberger
Neural stem and progenitor cells (NSPCs) generate neurons throughout life in the mammalian hippocampus. We used chronic in vivo imaging and followed genetically labeled individual NSPCs and their progeny in the mouse hippocampus for up to 2 months. We show that NSPCs targeted by the endogenous Achaete-scute homolog 1 (Ascl1) promoter undergo limited rounds of symmetric and asymmetric divisions, eliciting a burst of neurogenic activity, after which they are lost. Further, our data reveal unexpected asymmetric divisions of nonradial glia-like NSPCs...
February 9, 2018: Science
Alexander Edlich, Pierre Volz, Robert Brodwolf, Michael Unbehauen, Lars Mundhenk, Achim D Gruber, Sarah Hedtrich, Rainer Haag, Ulrike Alexiev, Burkhard Kleuser
Owing their unique chemical and physical properties core-multishell (CMS) nanocarriers are thought to underlie their exploitable biomedical use for a topical treatment of skin diseases. This highlights the need to consider not only the efficacy of CMS nanocarriers but also the potentially unpredictable and adverse consequences of their exposure thereto. As CMS nanocarriers are able to penetrate into viable layers of normal and stripped human skin ex vivo as well as in in vitro skin disease models the understanding of nanoparticle crosstalk with components of the immune system requires thorough investigation...
February 3, 2018: Biomaterials
Charmainne Cruje, Joy Dunmore-Buyze, Jarret P MacDonald, David W Holdsworth, Maria Drangova, Elizabeth R Gillies
Despite recent technological advancements in microcomputed tomography (micro-CT) and contrast agent development, pre-clinical contrast agents are still predominantly iodine-based. Higher contrast can be achieved when using elements with higher atomic numbers, such as lanthanides; lanthanides also have x-ray attenuation properties that are ideal for spectral CT. However, the formulation of lanthanide-based contrast agents at the high concentrations required for vascular imaging presents a significant challenge...
February 13, 2018: Biomacromolecules
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