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https://www.readbyqxmd.com/read/28743073/in-situ-biodegradation-potential-of-1-2-dca-and-vc-at-sites-with-different-hydrogeological-settings
#1
R C M Nobre, M M M Nobre, T M P Campos, D Ogles
This paper investigates the feasibility of applying in-situ Bioremediation (ISB) to three sites contaminated with vinyl chloride and/or chlorinated alkanes such as 1,2-DCA and 1,1,2-TCA, presenting distinct hydrogeological settings and history of contaminant loading. Biotransformation of these compounds is well established in laboratory studies and pure cultures. Due to confidential aspects, however, few field data are available to support real case studies to the predictability of their fate and lifetime in soil and groundwater...
July 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28742826/macromolecular-biosynthetic-parameters-and-metabolic-profile-in-different-life-stages-of-leishmania-braziliensis-amastigotes-as-a-functionally-less-active-stage
#2
Marlene Jara, Maya Berg, Guy Caljon, Geraldine de Muylder, Bart Cuypers, Denis Castillo, Ilse Maes, María Del Carmen Orozco, Manu Vanaerschot, Jean-Claude Dujardin, Jorge Arevalo
It was recently hypothesized that Leishmania amastigotes could constitute a semi-quiescent stage characterized by low replication and reduced metabolic activity. This concept developed with Leishmania (Leishmania) mexicana and Leishmania (Leishmania) major models might explain numerous clinical and sub-clinical features of Leishmania (Viannia) braziliensis infections, like reactivation of the disease, non-response to chemotherapy or asymptomatic infections. We compared here in vitro the proliferative capability of L...
2017: PloS One
https://www.readbyqxmd.com/read/28742812/broad-range-lytic-bacteriophages-that-kill-staphylococcus-aureus-local-field-strains
#3
Virginia Abatángelo, Natalia Peressutti Bacci, Carina A Boncompain, Ariel A Amadio, Soledad Carrasco, Cristian A Suárez, Héctor R Morbidoni
Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study...
2017: PloS One
https://www.readbyqxmd.com/read/28742249/transient-electroosmosis-of-a-maxwell-fluid-in-a-rotating-microchannel
#4
P Kaushik, Shubhadeep Mandal, Suman Chakraborty
The transient electroosmotic flow of Maxwell fluid in a rotating microchannel is investigated both analytically and numerically. We bring out the complex dynamics of the flow during the transience due to the combination of rotation and rheological effects. We show the regimes of operation under which our analysis holds the most significance. We also shed some light on the volumetric flow rate characteristics as dictated by the underlying flow physics. Mainly we show and analyze the various regimes of operation under which viscoelastic effects actuated by electroosmotic forcing dominate over Coriolis forces and vice versa, which has not been studied before...
July 25, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28742208/effects-of-microrna-21-targeting-pitx2-on-proliferation-and-apoptosis-of-pituitary-tumor-cells
#5
M Cui, M Zhang, H-F Liu, J-P Wang
OBJECTIVE: Expression of pituitary homeobox 2 (PITX2) is significantly elevated in pituitary adenoma tissues, which also has lower microRNA (miR)-21 expression, indicating possible tumor-suppression role of miR-21. Bio-informatics analysis revealed targeting onto 3'-UTR of PITX2 by miR-21. This study aims to investigate the role of miR-21/PITX2 expression in proliferation and apoptosis of pituitary adenoma cells and pathogenesis. PATIENTS AND METHODS: A total of 48 pituitary adenoma samples were collected in parallel with 12 normal brain tissues and were recruited in this study...
July 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28741693/antioxidants-protect-proteins-anchorage-to-the-bilayer-by-improving-plasma-membrane-integrity-of-ram-spermatozoa-during-liquid-preservation-in-a-soya-lecithin-based-diluent
#6
R K Paul, D Kumar, Smk Naqvi
Antioxidants are known to prevent the reactive oxygen species (ROS)-mediated peroxidative damage to the membrane lipids during hypothermic storage of mammalian spermatozoa. We hypothesized here that ROS also affect the lipid-protein interactions, thereby diminishing the membrane's integrity and proteins' anchorage to the bilayer. Antioxidants prevent these damages by scavenging the ROS. Ejaculates from Patanwadi rams were pooled after subjective evaluation and centrifuged using Percoll(®) . Sperm pellet was resuspended in soya lecithin-Tris-fructose diluent (400 × 10(6)  cells/ml) containing either antioxidants (100 IU/ml catalase + 10 mM reduced glutathione) or no antioxidant...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28741087/the-performance-of-different-anti-dsdna-autoantibodies-assays-in-chinese-systemic-lupus-erythematosus-patients
#7
Jiangfeng Zhao, Kaiwen Wang, Xiaodong Wang, Ting Li, Li Guo, Liyang Gu, Zhiwei Chen, Fangfang Sun, Haiting Wang, Jiajie Li, Jiaxian Huang, Peng Zhang, Yang Tang, Shuang Ye
To compare the performance of different commercial anti-dsDNA autoantibody assays, including multiplex-based immunoassay (Bio-Plex), Farr radioimmunoassay (Farr), ELISA, chemiluminescent immunoassay (CLIA), and Crithidia Luciliae indirect immunofluorescence test (CLIFT) in Chinese patients with systemic lupus erythematosus (SLE). SLE patients (n = 119) as well as healthy controls (n = 200) and disease controls (n = 100) were recruited, and serum anti-dsDNA autoantibodies were detected by Bio-Plex, Farr, two ELISA assays (Medical & Biological Laboratories-ELISA, EUROIMMU-ELISA), CLIA, and a standard CLIFT...
July 25, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28739445/surface-charge-dependent-separation-of-modified-and-hybrid-ferritin-in-native-page-impact-of-lysine-104
#8
Biswamaitree Subhadarshanee, Abhinav Mohanty, Manas Kumar Jagdev, Dileep Vasudevan, Rabindra K Behera
Preparation of modified and hybrid ferritin provides a great opportunity to understand the mechanisms of iron loading/unloading, protein self-assembly, size constrained nanomaterial synthesis and targeted drug delivery. However, the large size (M.W. = 490kDa) has been limiting the separation of different modified and/or hybrid ferritin nanocages from each other in their intact assembled form and further characterization. Native polyacrylamide gel electrophoresis (PAGE) separates proteins on the basis of both charge and mass, while maintaining their overall native structure and activity...
July 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28739409/fabrication-of-au-iii-ion-imprinted-polymer-based-on-thiol-modified-chitosan
#9
M Monier, D A Abdel-Latif
Au(III) ions were selectively extracted from aqueous media using Au(III) ion-imprinted bio-adsorbent based on modified chitosan (Au-CMB). First, 2-mercaptobenzaldehyde-chitosan Schiff base was prepared and interacted with Au(III) ions. The obtained polymeric Au(III) complex was then cross-linked by epichlorohydrin (ECH) before leaching the template Au(III) ions out of the cross-linked matrix. During the synthetic procedures, the obtained chitosan derivatives were characterized by Elemental analysis, FTIR and NMR spectra...
July 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28739341/atomic-force-microscope-as-a-nano-and-micrometer-scale-biological-manipulator-a-short-review
#10
REVIEW
Atsushi Ikai, Rehana Afrin, Masakazu Saito, Takahiro Watanabe-Nakayama
The amazing capacity of atomic force microscope to let us touch the molecular and cellular level samples with a sharp probe stimulated its application to bio-medical field among others. In addition to topographical imaging of the sample surface, a direct mechanical manipulation has attracted innovative minds to develop new methodologies aiming at direct handling of proteins, DNA/RNA, and cells. Measurement of their mechanical properties brought about a vivid picture of their physical nature. Direct handling of individual molecules and cells prompted development of nano-medical applications...
July 21, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28739302/comparison-of-the-analytical-and-clinical-performance-of-five-tests-for-the-detection-of-human-papillomavirus-genital-infection
#11
M Del Pino, I Alonso, A Trujillo, S Bernal, D Geraets, N Guimerà, A Torne, J Ordi
HPV-based screening provides greater protection against cervical cancer (CC) than cytology-based strategies. Currently, several molecular diagnostic assays for the detection of human papillomavirus (HPV) are available. In this study, we analyzed 5 different HPV testing and genotyping techniques (Hybrid Capture 2 [HC2; Qiagen, Hilden, Germany], AnyplexTMII HPV28 [Anyplex; Seegene, Seoul, Korea], Linear Array [Roche, Branchburg, NJ, USA], GP5+/6+ PCR-EIA-RH [Labo Bio-medical Products, Rijswijk, The Netherlands] and CLART2 [Genomica, Madrid, Spain]) in 295 women referred to the hospital Colposcopy Clinic from 2007 to 2008 due to positive HPV test results or an abnormal Pap test...
July 21, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28738661/semiautomatic-sequential-extraction-of-polycyclic-aromatic-hydrocarbons-and-elemental-bio-accessible-fraction-by-accelerated-solvent-extraction-on-a-single-particulate-matter-sample
#12
Maria Luisa Astolfi, Patrizia Di Filippo, Alessandra Gentili, Silvia Canepari
We describe the optimization and validation of a sequential extractive method for the determination of the polycyclic aromatic hydrocarbons (PAHs) and elements (Al, As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Se, V and Zn) that are chemically fractionated into bio-accessible and mineralized residual fractions on a single particulate matter filter. The extraction is performed by automatic accelerated solvent extraction (ASE); samples are sequentially treated with dichloromethane/acetone (4:1) for PAHs extraction and acetate buffer (0...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738629/innovative-simple-and-green-ultrasound-assisted-enzyme-based-hydrolytic-microextraction-method-for-manganese-at-trace-levels-in-food-samples
#13
Erkan Yilmaz, Mustafa Soylak
We describe the usage of hydrolytic enzymes for the innovative, green and simple microextraction of manganese in food samples. The ultrasound assisted-enzyme based hydrolytic microextraction method (UA-EH-ME) was applied for the separation and determination of manganese in food samples prior to its micro-sampling flame atomic absorption spectrometric determination (MS-FAAS). Analytical parameters influenced on the enzyme based hydrolytic microextraction method such as pH, type of enzyme, amount of enzyme, temperature and time of ultrasonic waves were fully evaluated and optimized by using mg level of NCS ZC73033 scallion certified reference material...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738550/employing-microbial-electrochemical-technology-driven-electro-fenton-oxidation-for-the-removal-of-recalcitrant-organics-from-sanitary-landfill-leachate
#14
Muhammad Hassan, Narcis Pous, Bing Xie, Jesús Colprim, M Dolors Balaguer, Sebastia Puig
The feasibility of employing Microbial Electrochemical Technology (MET)-driven electro-Fenton oxidation was evaluated as a post-treatment of an anammox system treating sanitary landfill leachate. Two different MET configuration systems were operated using effluent from partial nitrification-anammox reactor treating mature leachate. In spite of the low organic matter biodegradability of the anammox's effluent (2401±562mgCODL(-1); 237±57mgBOD5L(-1)), the technology was capable to reach COD removal rates of 1077-1244mgL(-1)d(-1) with concomitant renewable electricity production (43...
July 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738513/mitigation-of-nonpoint-source-pollution-in-rural-areas-from-control-to-synergies-of-multi-ecosystem-services
#15
Yonghong Wu, Junzhuo Liu, Renfang Shen, Bojie Fu
Nonpoint source (NPS) pollution produced by human activities in rural areas has induced excessive nutrient input into surface waters and the decline of water quality. The essence of NPS pollution is the transport of nutrients between soil and water. Traditional NPS pollution control strategies, however, are mainly based on the solid and liquid phases, with little focus on the bio-phase between water and soil. The pollutants produced from NPS can be regarded as a resource if recycled or reused in an appropriate way in the agricultural ecosystem...
July 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28738503/integration-of-protein-extraction-with-a-stream-of-byproducts-from-marine-macroalgae-a-model-forms-the-basis-for-marine-bioeconomy
#16
Tejal K Gajaria, Poornima Suthar, Ravi S Baghel, Nikunj B Balar, Preeti Sharnagat, Vaibhav A Mantri, C R K Reddy
The present study describes an advanced biorefinery model for marine macroalgae that assumes significant importance in the context of marine bio-economy. The method investigated in this study integrates the extraction of crude proteins with recovery of minerals rich sap, lipids, ulvan and cellulose from fresh biomass of Ulva lactuca. The protein content extracted was 11±2.12% on dry weight basis with recovery efficiency of 68.75±4.01%. The amino acid composition of crude protein fraction showed iso-leucine as the most abundant amino acid with 16...
July 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738303/bio-accumulation-of-lanthanum-from-lanthanum-modified-bentonite-treatments-in-lake-restoration
#17
G Waajen, F van Oosterhout, M Lürling
Lanthanum (La) modified bentonite (LMB) is one of the available mitigating agents used for the reduction of the phosphorus (P) recycling in eutrophic lakes. The potential toxicity of the La from LMB to aquatic organisms is a matter of concern. In this study the accumulation of La was investigated in the macrophyte Elodea nuttallii, in chironomid larvae and in several fish species during periods up to five years following in situ LMB applications. The application of LMB increased the La concentration of exposed plants and animals...
July 21, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28738174/distribution-of-ion-contents-and-microorganisms-during-the-electro-bioremediation-of-petroleum-contaminated-saline-soil
#18
Meng Zhang, Shuhai Guo, Fengmei Li, Bo Wu
This study investigated the distribution of ion contents and microorganisms during the electro-bioremediation (EK-Bio) of petroleum-contaminated saline soil. The results showed that soil ions tend to accumulate around the electrodes, and the concentration was correlated with the distance from the electrodes. The average soil ion content was 7.92 g/kg around the electrodes (site A) and 0.55 g/kg at the furthest distance from the electrodes (site B) after 112 days of treatment, while the initial average content was 3...
July 24, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28737701/application-of-extrusion-based-hydrogel-bioprinting-for-cartilage-tissue-engineering
#19
REVIEW
Fu You, B Frank Eames, Xiongbiao Chen
Extrusion-based bioprinting (EBB) is a rapidly developing technique that has made substantial progress in the fabrication of constructs for cartilage tissue engineering (CTE) over the past decade. With this technique, cell-laden hydrogels or bio-inks have been extruded onto printing stages, layer-by-layer, to form three-dimensional (3D) constructs with varying sizes, shapes, and resolutions. This paper reviews the cell sources and hydrogels that can be used for bio-ink formulations in CTE application. Additionally, this paper discusses the important properties of bio-inks to be applied in the EBB technique, including biocompatibility, printability, as well as mechanical properties...
July 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28737182/micro-and-nano-patterned-elastin-like-polypeptide-hydrogels-for-stem-cell-culture
#20
A Paul, M Stührenberg, S Chen, D Rhee, W-K Lee, T W Odom, S C Heilshorn, A Enejder
We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy...
July 24, 2017: Soft Matter
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