keyword
MENU ▼
Read by QxMD icon Read
search

bio

keyword
https://www.readbyqxmd.com/read/28346420/hepatic-cyclooxygenase-2-overexpression-induced-spontaneous-hepatocellular-carcinoma-formation-in-mice
#1
H Chen, W Cai, E S H Chu, J Tang, C-C Wong, S H Wong, W Sun, Q Liang, J Fang, Z Sun, J Yu
Cyclooxygenase (COX)-2 is upregulated in hepatocellular carcinoma (HCC). However, the direct causative effect of COX-2 in spontaneous HCC formation remains unknown. We thus investigate the role and molecular pathogenesis of COX-2 in HCC by using liver-specific COX-2 transgenic (TG) mice. We found spontaneous HCC formation with elevated inflammatory infiltrates and neovessels in male TG mice (3/21, 14.3%), but not in any of male WT mice (0/19). Reduced representation bisulfite sequencing (RRBS) and gene expression microarrays were performed in the HCC tumor and non-HCC liver tissues to investigate the molecular mechanisms of COX-2-driven HCC...
March 27, 2017: Oncogene
https://www.readbyqxmd.com/read/28346372/-bligh-and-dyer-and-folch-methods-for-solid-liquid-liquid-extraction-of-lipids-from-microorganisms-comprehension-of-solvatation-mechanisms-and-towards-substitution-with-alternative-solvents
#2
Cassandra Breil, Maryline Abert Vian, Thomas Zemb, Werner Kunz, Farid Chemat
Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid-liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid-liquid partition-the two steps of routine extraction...
March 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28345880/detecting-proteins-glycosylation-by-a-homogeneous-reaction-system-with-zwitterionic-gold-nanoclusters
#3
Jinan Li, Jing Liu, Zheyi Liu, Yuan Tan, Xiaoyan Liu, Fangjun Wang
Homogeneous gold nanoclusters (Au NCs) have been widely utilized in drug delivery, chemical sensing, bioassays and bio-labeling due to their unique physical and chemical properties. However, little attention has been paid to their application in detecting protein post-translational modifications. Herein, we describe development of a homogeneous reaction system with water-soluble zwitterionic Au NCs to capture glycopeptides from complex biological samples. The unique characteristics of Au NCs, such as their molecular-like properties, the excellent homogeneity in aqueous solution, the organic solvent respon-sive precipitation, and the easy preparation in only 4...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28345862/quantification-of-lipid-metabolism-in-living-cells-through-the-dynamics-of-lipid-droplets-measured-by-stimulated-raman-scattering-imaging
#4
Chi Zhang, Junjie Li, Lu Lan, Ji-Xin Cheng
Dysregulation of lipid metabolism is associated with many diseases including cancer. Lipid droplet (LD), a ubiquitous organelle in mammalian cells, serves as a hub for lipid metabolism. Conventional assays on the measurement of lipid metabolism rely on the quantification of the lipid composition or amount. Such methods cannot distinguish LDs having different bio-functionalities in living cells, and thus could be inaccurate in measuring the instantaneous lipogenesis of the living cells. We applied label-free stimulated Raman scattering microscopy to quantify the LDs' spatial-temporal dynamics, which showed direct links to cellular lipid metabolisms and can separate LDs involving in different metabolic events...
March 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28345745/in-vitro-and-in-vivo-assessment-of-the-abuse-potential-of-pf614-a-novel-bio-md%C3%A2-prodrug-of-oxycodone
#5
D Lynn Kirkpatrick, William K Schmidt, Ricardo Morales, John Cremin, Julie Seroogy, Craig Husfeld, Thomas Jenkins
OBJECTIVE: The need for pain medication which will not lead to abuse is well recognized. Ensysce has designed prodrug analogs of the commonly used pain medications including hydromorphone, oxycodone (OC), hydrocodone, and morphine that limit their use to oral delivery, two of which are in clinical development. This study was undertaken to demonstrate that PF614, an extended-release prodrug of OC, allows the release of OC as designed when delivered orally, yet it resists ex vivo extraction with household chemicals and is pharmacologically inactive when administered by nonoral routes (nasal and parenteral), thereby substantially reducing its intravenous (IV) and intranasal abuse potential...
January 2017: Journal of Opioid Management
https://www.readbyqxmd.com/read/28345715/ultralow-power-near-infrared-excited-neodymium-doped-nanoparticles-for-long-term-in-vivo-bioimaging
#6
Qing-Song Qin, Pei-Zhi Zhang, Ling-Dong Sun, Shuo Shi, Nai-Xiu Chen, Hao Dong, Xiao-Yu Zheng, Le-Min Li, Chun-Hua Yan
Lanthanide-doped luminescent nanoparticles with both emission and excitation in the near-infrared (NIR-to-NIR) region hold great promise for bioimaging. Herein, core@shell structured LiLuF4:Nd@LiLuF4 (named as Nd@Lu) nanoparticles (NPs) with highly efficient NIR emission were developed for high-performance in vivo bioimaging. Strikingly, the absolute quantum yield of Nd@Lu NPs reached as high as 32%. After coating with polyethylene glycol (PEG), the water-dispersible Nd@Lu NPs showed good bio-compatibility and low toxicity...
March 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28345669/a-coupled-human-earth-model-perspective-on-long-term-trends-in-the-global-marine-fishery
#7
E D Galbraith, D A Carozza, D Bianchi
The global wild marine fish harvest increased fourfold between 1950 and a peak value near the end of the 20th century, reflecting interactions between anthropogenic and ecological forces. Here, we examine these interactions in a bio-energetically constrained, spatially and temporally resolved model of global fisheries. We conduct historical hindcasts with the model, which suggest that technological progress can explain most of the 20th century increase of fish harvest. In contrast, projections extending this rate of technological progress into the future under open access suggest a long-term decrease in harvest due to over-fishing...
March 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28345585/direct-amidation-of-esters-with-nitroarenes
#8
Chi Wai Cheung, Marten Leendert Ploeger, Xile Hu
Esters are one of the most common functional groups in natural and synthetic products, and the one-step conversion of the ester group into other functional groups is an attractive strategy in organic synthesis. Direct amidation of esters is particularly appealing due to the omnipresence of the amide moiety in biomolecules, fine chemicals, and drug candidates. However, efficient methods for direct amidation of unactivated esters are still lacking. Here we report nickel-catalysed reductive coupling of unactivated esters with nitroarenes to furnish in one step a wide range of amides bearing functional groups relevant to the development of drugs and agrochemicals...
March 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28345386/artifact-correction-in-temperature-dependent-attenuated-total-reflection-infrared-atr-ir-spectra
#9
Brian Sobieski, Bruce Chase, Isao Noda, John Rabolt
A spectral processing method was developed and tested for analyzing temperature-dependent attenuated total reflection infrared (ATR-IR) spectra of aliphatic polyesters. Spectra of a bio-based, biodegradable polymer, 3.9 mol % 3HHx poly[(R)-3-hydroxybutyrate- co-(R)-3-hydroxyhexanoate] (PHBHx), were analyzed and corrected prior to analysis using two-dimensional correlation spectroscopy (2D-COS). Removal of the temperature variation of diamond absorbance, correction of the baseline, ATR correction, and appropriate normalization were key to generating more reliable data...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28345352/metal-free-photoinduced-electron-transfer-atom-transfer-radical-polymerization-integrated-with-bio-inspired-polydopamine-chemistry-as-a-green-strategy-for-surface-engineering-of-magnetic-nanoparticles
#10
Yang Yang, Xuegang Liu, Gang Ye, Shan Zhu, Zhe Wang, Xiaomei Huo, Krzysztof Matyjaszewski, Yuexiang Lu, Jing Chen
Developing green and efficient technologies for surface modification of magnetic nanoparticles (MNPs) is of crucial importance for their biomedical and environmental applications. This study reports, for the first time, a novel strategy by integrating metal-free photoinduced electron transfer-atom transfer radical polymerization (PET-ATRP) with the bio-inspired polydopamine (PDA) chemistry for controlled architecture of functional polymer brushes from MNPs. Conformal PDA encapsulation layers were initially generated on the surfaces of MNPs, which served as the protective shells while providing an ideal platform for tethering 2-bromo-2-phenylacetic acid (BPA), a highly efficient initiator...
March 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28344727/characterization-of-vascular-permeability-using-a-biomimetic-microfluidic-blood-vessel-model
#11
Antony Thomas, Shunqiang Wang, Salman Sohrabi, Colin Orr, Ran He, Wentao Shi, Yaling Liu
The inflammatory response in endothelial cells (ECs) leads to an increase in vascular permeability through the formation of gaps. However, the dynamic nature of vascular permeability and external factors involved is still elusive. In this work, we use a biomimetic blood vessel (BBV) microfluidic model to measure in real-time the change in permeability of the EC layer under culture in physiologically relevant flow conditions. This platform studies the dynamics and characterizes vascular permeability when the EC layer is triggered with an inflammatory agent using tracer molecules of three different sizes, and the results are compared to a transwell insert study...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28344110/toward-a-systematic-exploration-of-nano-bio-interactions
#12
REVIEW
Xue Bai, Fang Liu, Yin Liu, Cong Li, Shenqing Wang, Hongyu Zhou, Wenyi Wang, Hao Zhu, Dave Winkler, Bing Yan
Many studies of nanomaterials make non-systematic alterations of nanoparticle physicochemical properties. Given the immense size of the property space for nanomaterials, such approaches are not very useful in elucidating fundamental relationships between inherent physicochemical properties of these materials and their interactions with, and effects on, biological systems. Data driven artificial intelligence methods such as machine learning algorithms have proven highly effective in generating models with good predictivity and some degree of interpretability...
March 24, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28343651/reconstruction-of-large-cranial-defects-with-poly-methyl-methacrylate-pmma-using-a-rapid-prototyping-model-and-a-new-technique-for-intraoperative-implant-modeling
#13
Claudia Unterhofer, Christoph Wipplinger, Michael Verius, Wolfgang Recheis, Claudius Thomé, Martin Ortler
BACKGROUND: Reconstruction of large cranial defects after craniectomy can be accomplished by free-hand poly-methyl-methacrylate (PMMA) or industrially manufactured implants. The free-hand technique often does not achieve satisfactory cosmetic results but is inexpensive. In an attempt to combine the accuracy of specifically manufactured implants with low cost of PMMA. METHODS: Forty-six consecutive patients with large skull defects after trauma or infection were retrospectively analyzed...
March 10, 2017: Neurologia i Neurochirurgia Polska
https://www.readbyqxmd.com/read/28343560/acoustic-levitation-of-liquid-drops-dynamics-manipulation-and-phase-transitions
#14
REVIEW
Duyang Zang, Yinkai Yu, Zhen Chen, Xiaoguang Li, Hongjing Wu, Xingguo Geng
The technique of acoustic levitation normally produces a standing wave and the potential well of the sound field can be used to trap small objects. Since no solid surface is involved it has been widely applied for the study of fluid physics, nucleation, bio/chemical processes, and various forms of soft matter. In this article, we survey the works on drop dynamics in acoustic levitation, focus on how the dynamic behavior is related to the rheological properties and discuss the possibility to develop a novel rheometer based on this technique...
March 18, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28342814/tear-cytokine-profile-of-glaucoma-patients-treated-with-preservative-free-or-preserved-latanoprost
#15
Jose M Martinez-de-la-Casa, Francisco Perez-Bartolome, Elena Urcelay, Jose L Santiago, Javier Moreno-Montañes, Pedro Arriola-Villalobos, Jose M Benitez-Del-Castillo, Julian Garcia-Feijoo
PURPOSE: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. METHODS: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF)...
March 22, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28342590/a-life-cycle-assessment-of-distributed-energy-production-from-organic-waste-two-case-studies-in-europe
#16
Sara Evangelisti, Roland Clift, Carla Tagliaferri, Paola Lettieri
By means of the life cycle assessment methodology, the purpose of this study is to assess the environmental impact when biomethane from organic waste produced at residential level is used to supply energy to a group of dwellings in the distributed generation paradigm. Three different Combined Heat and Power systems, such as fuel cells, Stirling engine and micro gas turbine, installed at household level are assessed in two different settings: one in Northern Europe (UK) and one in Southern Europe (Italy). Different operating strategies are investigated for each technology...
March 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28342566/mechanical-and-biological-effects-of-ultrasound-a-review-of-present-knowledge
#17
REVIEW
Zahra Izadifar, Paul Babyn, Dean Chapman
Ultrasound is widely used for medical diagnosis and increasingly for therapeutic purposes. An understanding of the bio-effects of sonography is important for clinicians and scientists working in the field because permanent damage to biological tissues can occur at high levels of exposure. Here the underlying principles of thermal mechanisms and the physical interactions of ultrasound with biological tissues are reviewed. Adverse health effects derived from cellular studies, animal studies and clinical reports are reviewed to provide insight into the in vitro and in vivo bio-effects of ultrasound...
March 22, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28342406/phase-change-heat-transfer-during-cryosurgery-of-lung-cancer-using-hyperbolic-heat-conduction-model
#18
Ajay Kumar, Sushil Kumar, V K Katiyar, Shirley Telles
The paper reports a numerical study of phase change heat transfer process in lung cancer undergoing cryosurgery. A two dimensional hyperbolic bio-heat model with non-ideal property of tissue, blood perfusion and metabolism is used to analyze the problem. The governing equations are solved by finite difference method based on enthalpy formulation. Effects of relaxation time of heat flux in hyperbolic model on freezing process have been examined. A comparative investigation of two different models (hyperbolic and parabolic bio-heat models) is also presented...
March 16, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28342375/nanozyme-based-bio-barcode-assay-for-high-sensitive-and-logic-controlled-specific-detection-of-multiple-dnas
#19
Xiaodong Lin, Yaqing Liu, Zhanhui Tao, Jinting Gao, Jiankang Deng, Jinjin Yin, Shuo Wang
Since HCV and HIV share a common transmission path, high sensitive detection of HIV and HCV gene is of significant importance to improve diagnosis accuracy and cure rate at early stage for HIV virus-infected patients. In our investigation, a novel nanozyme-based bio-barcode fluorescence amplified assay is successfully developed for simultaneous detection of HIV and HCV DNAs with excellent sensitivity in an enzyme-free and label-free condition. Here, bimetallic nanoparticles, PtAuNPs, present outstanding peroxidase-like activity and act as barcode to catalyze oxidation of nonfluorescent substrate of amplex red (AR) into fluorescent resorufin generating stable and sensitive "Turn On" fluorescent output signal, which is for the first time to be integrated with bio-barcode strategy for fluorescence detection DNA...
January 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28342340/cellulose-decomposition-and-larval-biomass-production-from-the-co-digestion-of-dairy-manure-and-chicken-manure-by-mini-livestock-hermetia-illucens-l
#20
Kashif Ur Rehman, Minmin Cai, Xiaopeng Xiao, Longyu Zheng, Hui Wang, Abdul Aziz Soomro, Yusha Zhou, Wu Li, Ziniu Yu, Jibin Zhang
World trends toward the modern dairies intensification on large production units cause massive animal manure production and accumulation. Improper handling of manure produced by industrial farm operation greatly deteriorates the major environmental media including air, water and soil. The black soldier fly utilizes organic waste and converts it into larvae biomass to be used as livestock feed and into residues to be used as bio-fertilizer. However, due to the high ratio of cellulose, hemicellulose and lignin in dairy manure, this conversion is difficult...
March 22, 2017: Journal of Environmental Management
keyword
keyword
38237
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"