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https://www.readbyqxmd.com/read/27925692/a-method-to-generate-and-analyze-modified-myristoylated-proteins
#1
Huanyao Gao, Wei Sun, Zhiquan Song, Yanbao Yu, Li Wang, Xian Chen, Qisheng Zhang
Covalent lipid modification of proteins is essential to their cellular localizations and functions. Engineered lipid motif coupled with bio-orthogonal chemistry has been utilized to identify myristoylated or palmitoylated proteins in cells. However, whether modified proteins have similar properties as endogenous ones has not been well investigated mainly due to lack of methods to generate and analyze purified proteins. We have developed a method that utilizes metabolic interference and mass spectrometry to produce and analyze modified, myristoylated small GTPase ADP-ribosylation factor 1 (Arf1)...
December 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27925592/enzyme-free-scalable-dna-digital-design-techniques-a-review
#2
Aby Konampurath George, Harpreet Singh
With the recent developments in DNA nanotechnology, DNA has been used as the basic building block for the design of nanostructures, autonomous molecular motors, various devices, and circuits. DNA is considered as a possible candidate for replacing silicon for designing digital circuits in a near future, especially in implantable medical devices, because of its parallelism, computational powers, small size, light weight, and compatibility with bio-signals. The research in DNA digital design is in early stages of development, and electrical and computer engineers are not much attracted towards this field...
December 2, 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/27925497/anti-inflammatory-and-anticancer-effects-of-flavonol-glycosides-from-diplotaxis-harra-through-gsk3%C3%AE-regulation-in-intestinal-cells
#3
Imen Nasri, Rachid Chawech, Cynthia Girardi, Emmanuel Mas, Audrey Ferrand, Nathalie Vergnolle, Nicolas Fabre, Raoudha Mezghani-Jarraya, Claire Racaud-Sultan
CONTEXT AND OBJECTIVE: Diplotaxis harra (Forssk.) Boiss. (Brassicaceae) is traditionally used as an antidiabetic, anti-inflammatory or anticancer agent. In these pathologies, the glycogen synthase kinase 3 β (GSK3β) is overactivated and represents an interesting therapeutic target. Several flavonoids can inhibit GSK3β and the purpose of this study was to search for the compounds in Diplotaxis harra which are able to modulate GSK3β. MATERIALS AND METHODS: Methanol extracts from D...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27925410/unraveling-the-role-of-formic-acid-and-the-type-of-solvent-in-the-catalytic-conversion-of-lignin-a-holistic-approach
#4
Mikel Oregui Bengoechea, Iñaki Gandarias, Pedro Luis Arias, Tanja Barth
The role of formic acid together with the effect of the solvent type and their synergic interactions with a NiMo catalyst were studied for the conversion of lignin into bio-oil in an alcohol/formic acid media. Replacing formic acid with molecular hydrogen or isopropanol decreased the oil yield to a considerable degree, increased the solid yield and altered the nature of the bio-oil. The differences induced by the presence of molecular hydrogen were comparable to the ones observed in the isopropanol system, suggesting similar lignin conversion mechanisms for both systems...
December 7, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27925173/potential-of-long-non-coding-rnas-in-cancer-patients-from-bio-markers-to-therapeutic-targets
#5
REVIEW
Subash Chandra Gupta, Yashoda Nandan Tripathi
Because of high specificity and easy detection in the tissues, serum, plasma, urine and saliva, interest in exploring the potential of long non-coding RNAs (lncRNAs) in cancer patients continues to increase. LncRNAs have shown potential as a bio-marker in the diagnosis and prognosis of bladder cancer, prostate cancer, gastric cancer, pancreatic cancer, breast cancer, and many other cancer types. Some lncRNAs have also been used as adjunct to improve the specificity and sensitivity of existing biomarkers. The molecular tools such as RNA-seq, RNA-FISH, ic-SHAPE and quantitative real-time PCR have been used for examining the lncRNAs' potential...
December 7, 2016: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27924952/a-competitive-bio-barcode-amplification-immunoassay-for-small-molecules-based-on-nanoparticles
#6
Pengfei Du, Maojun Jin, Ge Chen, Chan Zhang, Zejun Jiang, Yanxin Zhang, Pan Zou, Yongxin She, Fen Jin, Hua Shao, Shanshan Wang, Lufei Zheng, Jing Wang
A novel detection method of small molecules, competitive bio-barcode amplification immunoassay, was developed and described in this report. Through the gold nanoparticles (AuNPs) probe and magnetic nanoparticles (MNPs) probe we prepared, only one monoclonal antibody can be used to detect small molecules. The competitive bio-barcode amplification immunoassay overcomes the obstacle that the bio-barcode assay cannot be used in small molecular detection, as two antibodies are unable to combine to one small molecule due to its small molecular structure...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924940/the-metabolic-flux-regulation-of-klebsiella-pneumoniae-based-on-quorum-sensing-system
#7
Shujing Sun, Haiyang Zhang, Shuyi Lu, Chunfen Lai, Huijun Liu, Hu Zhu
Quorum-sensing (QS) systems exist universally in bacteria to regulate multiple biological functions. Klebsiella pneumoniae, an industrially important bacterium that produces bio-based chemicals such as 2,3-butanediol and acetoin, can secrete a furanosyl borate diester (AI-2) as the signalling molecule mediating a QS system, which plays a key regulatory role in the biosynthesis of secondary metabolites. In this study, the molecular regulation and metabolic functions of a QS system in K. pneumoniae were investigated...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27924784/bio-fabrication-of-nanomesh-channels-of-single-walled-carbon-nanotubes-for-locally-gated-field-effect-transistors
#8
Hye-Hyeon Byeon, Woo Chul Lee, Wonbin Kim, Seong Keun Kim, Woong Kim, Hyunjung Yi
Single-walled carbon nanotubes (SWNTs) are one of the promising electronic components for nanoscale electronic devices such as field-effect transistors (FETs) owing to their excellent device characteristics such as high conductivity, high carrier mobility and mechanical flexibility. Localized gating gemometry of FETs enables individual addressing of active channels and allows for better electrostatics via thinner dielectric layer of high k-value. For localized gating of SWNTs, it becomes critical to define SWNTs of controlled nanostructures and functionality onto desired locations in high precision...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27924782/resistive-switching-of-pt-tio-x-pt-devices-fabricated-on-flexible-parylene-c-substrates
#9
Ali Khiat, Simone Cortese, Alexander Serb, Themistoklis Prodromakis
Pt/TiO x /Pt resistive switching (RS) devices are considered to be amongst the most promising candidates in memristor family and the technology transfer to flexible substrates could open the way to new opportunities for flexible memory implementations. Hence, an important goal is to achieve a fully flexible RS memory technology. Nonetheless, several fabrication challenges are present and must be solved prior to achieving reliable device fabrication and good electronic performances. Here, we propose a fabrication method for the successful transfer of Pt/TiO x /Pt stack onto flexible Parylene-C substrates...
January 13, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27924490/site-specific-fluorescent-labeling-of-argonaute-for-fret-based-bio-assays
#10
Sarah Willkomm, Adrian Zander, Dina Grohmann
Deciphering the molecular mechanisms of eukaryotic Argonaute proteins is crucial for the understanding of RNA interference (RNAi), a posttranscriptional gene silencing process. Fluorescence-based single-molecule studies like single-molecule Förster resonance energy transfer (FRET) between a donor and acceptor dye represent a versatile tool to gain a mechanistic understanding of the structural dynamics of a biomolecular complex. Until today it was not possible to site-specifically introduce fluorophores into eukaryotic Argonaute...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924334/a-protein-corona-enabled-blood-test-for-early-cancer-detection
#11
D Caputo, M Papi, R Coppola, S Palchetti, L Digiacomo, G Caracciolo, D Pozzi
Pancreatic cancer is a very aggressive malignancy that is often diagnosed in the advanced stages, with the implication that long-term survivors are extremely rare. Thus, developing new methods for the early detection of pancreatic cancer is an urgent task for current research. To date, nanotechnology offers unprecedented opportunities for cancer therapeutics and diagnosis. The aim of this study is the development of a new pancreatic cancer diagnostic technology based on the exploitation of the nano-bio-interactions between nanoparticles and blood samples...
December 7, 2016: Nanoscale
https://www.readbyqxmd.com/read/27924256/operating-principles-of-rotary-molecular-motors-differences-between-f1-and-v1-motors
#12
REVIEW
Ichiro Yamato, Yoshimi Kakinuma, Takeshi Murata
Among the many types of bioenergy-transducing machineries, F- and V-ATPases are unique bio- and nano-molecular rotary motors. The rotational catalysis of F1-ATPase has been investigated in detail, and molecular mechanisms have been proposed based on the crystal structures of the complex and on extensive single-molecule rotational observations. Recently, we obtained crystal structures of bacterial V1-ATPase (A3B3 and A3B3DF complexes) in the presence and absence of nucleotides. Based on these new structures, we present a novel model for the rotational catalysis mechanism of V1-ATPase, which is different from that of F1-ATPases...
2016: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/27924146/mindfulness-based-intervention-for-female-adolescents-with-chronic-pain-a-pilot-randomized-trial
#13
Nicholas Chadi, Audrey McMahon, Majorie Vadnais, Catherine Malboeuf-Hurtubise, Anissa Djemli, Patricia L Dobkin, Jacques Lacroix, Thuy Mai Luu, Nancy Haley
OBJECTIVE: To test the feasibility of a randomized-controlled trial measuring the impact of an adapted mindfulness-based intervention (MBI) in female adolescents with chronic pain. METHODS: This was a single center, single-blind, prospective, experimental, longitudinal trial conducted in a pediatric tertiary care center. Participants had a history of chronic pain during at least three months. They were randomized into an intervention group or a wait-list control group...
2016: Journal of the Canadian Academy of Child and Adolescent Psychiatry
https://www.readbyqxmd.com/read/27924021/aagatlas-1-0-a-human-autoantigen-database
#14
Dan Wang, Liuhui Yang, Ping Zhang, Joshua LaBaer, Henning Hermjakob, Dong Li, Xiaobo Yu
Autoantibodies refer to antibodies that target self-antigens, which can play pivotal roles in maintaining homeostasis, distinguishing normal from tumor tissue and trigger autoimmune diseases. In the last three decades, tremendous efforts have been devoted to elucidate the generation, evolution and functions of autoantibodies, as well as their target autoantigens. However, reports of these countless previously identified autoantigens are randomly dispersed in the literature. Here, we constructed an AAgAtlas database 1...
October 19, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27924016/bio-tds-bioscience-query-tool-discovery-system
#15
Etienne Z Gnimpieba, Menno S VanDiermen, Shayla M Gustafson, Bill Conn, Carol M Lushbough
Bioinformatics and computational biology play a critical role in bioscience and biomedical research. As researchers design their experimental projects, one major challenge is to find the most relevant bioinformatics toolkits that will lead to new knowledge discovery from their data. The Bio-TDS (Bioscience Query Tool Discovery Systems, http://biotds.org/) has been developed to assist researchers in retrieving the most applicable analytic tools by allowing them to formulate their questions as free text. The Bio-TDS is a flexible retrieval system that affords users from multiple bioscience domains (e...
October 18, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27923385/field-effectiveness-of-microbial-larvicides-on-mosquito-larvae-in-malaria-areas-of-botswana-and-zimbabwe
#16
Mulamuli Mpofu, Piet Becker, Kaka Mudambo, Christiaan de Jager
BACKGROUND: The successful control of malaria vectors requires the control of both the larval and adult stages. The adult control methods through indoor residual spraying (IRS) and use of long-lasting insecticidal nets (LLINs) continue to be widely used with some high measure of success. Larval control methods are also being used by a number of National Malaria Control Programmes (NMCPs) with limited understanding of its contribution. Larval control might be needed in some areas to move from malaria control to elimination...
December 6, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27922209/unconventional-pretreatment-of-lignocellulose-with-low-temperature-plasma
#17
Jens Vanneste, Thijs Ennaert, Annick Vanhulsel, Bert F Sels
Lignocellulose represents a potential supply of sustainable feedstock for the production of biofuels and chemicals. There is, however, an important cost and efficiency challenge associated with the conversion of such lignocellulosics. Since its structure is complex and not prone to undergo chemical reactions very easily, chemical and mechanical pretreatments are usually necessary in order to be able to refine them into the compositional building blocks (carbohydrates and lignin), from which value-added platform molecules such as glucose, ethylene glycol, 5-hydroxymethylfurfural, levulinic acid, etc...
December 6, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27922072/molecular-structure-and-dynamics-of-water-on-pristine-and-strained-phosphorene-wetting-and-diffusion-at-nanoscale
#18
Wei Zhang, Chao Ye, Linbi Hong, Zaixing Yang, Ruhong Zhou
Phosphorene, a newly fabricated two-dimensional (2D) nanomaterial, has emerged as a promising material for biomedical applications with great potential. Nonetheless, understanding the wetting and diffusive properties of bio-fluids on phosphorene which are of fundamental importance to these applications remains elusive. In this work, using molecular dynamics (MD) simulations, we investigated the structural and dynamic properties of water on both pristine and strained phosphorene. Our simulations indicate that the diffusion of water molecules on the phosphorene surface is anisotropic, with strain-enhanced diffusion clearly present, which arises from strain-induced smoothing of the energy landscape...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922065/detc-based-bacterial-cellulose-bio-curatives-for-topical-treatment-of-cutaneous-leishmaniasis
#19
Fabiana S Celes, Eliane Trovatti, Ricardo Khouri, Johan Van Weyenbergh, Sidney J L Ribeiro, Valeria M Borges, Hernane S Barud, Camila I de Oliveira
The treatment of leishmaniasis still relies on drugs with potentially serious adverse effects. Herein, we tested a topical formulation of bacterial cellulose (BC) membranes containing Diethyldithiocarbamate (DETC), a superoxide dismutase 1 inhibitor. Leishmania-infected macrophages exposed to BC-DETC resulted in parasite killing, without pronounced toxic effects to host cells. This outcome was associated with lower SOD1 activity and higher production of superoxide and cytokine mediators. Topical application of BC-DETC significantly decreased lesion size, parasite load and the inflammatory response at the infection site, as well as the production of both IFN-γ and TNF...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922059/protein-self-assembly-onto-nanodots-leads-to-formation-of-conductive-bio-based-hybrids
#20
Xiao Hu, Chenbo Dong, Rigu Su, Quan Xu, Cerasela Zoica Dinu
The next generation of nanowires that could advance the integration of functional nanosystems into synthetic applications from photocatalysis to optical devices need to demonstrate increased ability to promote electron transfer at their interfaces while ensuring optimum quantum confinement. Herein we used the biological recognition and the self-assembly properties of tubulin, a protein involved in building the filaments of cellular microtubules, to create stable, free standing and conductive sulfur-doped carbon nanodots-based conductive bio-hybrids...
December 6, 2016: Scientific Reports
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