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https://www.readbyqxmd.com/read/29346727/bioinspired-pressure-tolerant-asymmetric-slippery-surface-for-continuous-self-transport-of-gas-bubbles-in-aqueous-environment
#1
Chunhui Zhang, Bo Zhang, Hongyu Ma, Zhe Li, Xiao Xiao, Yuheng Zhang, Xinyu Cui, Cunming Yu, Moyuan Cao, Lei Jiang
Bio-surfaces with geometry-gradient structures or special wettabilities demonstrate intriguing performance in manipulating the behaviors of versatile fluids. By mimicking natural species, i.e., the cactus spine with a shape-gradient morphology and the Picher plant with a lubricated inner surface, we have successfully prepared an asymmetric slippery surface by following the processes of CO2-laser cutting, superhydrophobic modification, and the fluorinert infusion. The asymmetric morphology will cause the deformation of gas bubbles and subsequently engender an asymmetric driven force on them...
January 18, 2018: ACS Nano
https://www.readbyqxmd.com/read/29346685/metabolic-flux-engineering-of-cembratrien-ol-production-in-both-the-glandular-trichome-and-leaf-mesophyll-in-nicotiana-tabacum
#2
Hongying Zhang, Songtao Zhang, Yongxia Yang, Hongfang Jia, Hong Cui
Cembratrien-ol synthase (CBTS) catalyses the first step in cembranoid biosynthesis, producing cembratrien-ols in plant trichomes. In our previous study, microarray transcriptomes between leaves with trichomes and leaves without trichomes showed that a NtCBTS2 gene was expressed exclusively and abundantly in trichomes. Here, two NtCBTS2 isogenes (NtCBTS2a and NtCBTS2b), derived from a diploid genome donor, N. sylvestris, were identified from N. tabacum. Both genes were expressed primarily in trichomes, with relatively decreased transcription in flowers and stems, faint expression in roots, and no expression was detected in leaves lacking trichomes...
January 15, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29346641/uncovering-behavioural-diversity-amongst-high-strength-pseudomonas-spp-surfactants-at-the-limit-of-liquid-surface-tension-reduction
#3
Kamaluddeen Kabir, Yusuf Y Deeni, Simona M Hapca, Luke Moore, Andrew J Spiers
Bacterial bio-surfactants have a wide range of biological functions and biotechnological applications. Previous analyses had suggested a limit to their reduction of aqueous liquid surface tensions (γMin), and here we confirm this in an analysis of 25 Pseudomonas spp. strains isolated from soil which produce high-strength surfactants that reduce surface tensions to 25.2 ± 0.1-26.5 ± 0.2 mN.m-1 (the surface tension of sterile growth medium and pure water was 52.9 ± 0.4 mN.m-1 and 72.1 ± 1.2 mN.m-1, respectively)...
January 16, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29346562/scaffold-free-trachea-regeneration-by-tissue-engineering-with-bio-3d-printing
#4
Daisuke Taniguchi, Keitaro Matsumoto, Tomoshi Tsuchiya, Ryusuke Machino, Yosuke Takeoka, Abdelmotagaly Elgalad, Kiyofumi Gunge, Katsunori Takagi, Yasuaki Taura, Go Hatachi, Naoto Matsuo, Naoya Yamasaki, Koichi Nakayama, Takeshi Nagayasu
OBJECTIVES: Currently, most of the artificial airway organs still require scaffolds; however, such scaffolds exhibit several limitations. Alternatively, the use of an autologous artificial trachea without foreign materials and immunosuppressants may solve these issues and constitute a preferred tool. The rationale of this study was to develop a new scaffold-free approach for an artificial trachea using bio-3D printing technology. Here, we assessed the circumferential tracheal replacement using scaffold-free trachea-like grafts generated from isolated cells in an inbred animal model...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29346404/bio-fortification-potential-of-global-wild-annual-lentil-core-collection
#5
Sandeep Kumar, Anil Kumar Choudhary, Kuldeep Singh Rana, Ashutosh Sarker, Mohar Singh
Lentil, generally known as poor man's' meat due to its high protein value is also a good source of dietary fiber, antioxidants and vitamins along with fast cooking characteristics. It could be used globally as a staple food crop to eradicate hidden hunger, if this nutritionally rich crop is further enriched with essential minerals. This requires identification of essential mineral rich germplasm. So, in the present study, a core set of 96 wild accessions extracted from 405 global wild annual collections comprising different species was analyzed to determine its bio-fortification potential...
2018: PloS One
https://www.readbyqxmd.com/read/29346085/performance-of-the-bioplex-2200-hiv-ag-ab-assay-for-identifying-acute-hiv-infection
#6
Susan H Eshleman, Estelle Piwowar-Manning, Mariya V Sivay, Barbara Debevec, Stephanie Veater, Laura McKinstry, Linda-Gail Bekker, Sharon Mannheimer, Robert M Grant, Margaret A Chesney, Thomas J Coates, Beryl A Koblin, Jessica M Fogel
BACKGROUND: Assays that detect HIV antigen (Ag) and antibody (Ab) can be used to screen for HIV infection. OBJECTIVES: To compare the performance of the BioPlex 2200 HIV Ag-Ab assay and two other Ag/Ab combination assays for detection of acute HIV infection. STUDY DESIGN: Samples were obtained from 24 individuals (18 from the US, 6 from South Africa); these individuals were classified as having acute infection based on the following criteria: positive qualitative RNA assay; two negative rapid tests; negative discriminatory test...
January 11, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29344958/protective-effects-of-new-antioxidant-compositions-of-4-methylcoumarins-and-related-compounds-with-dl-%C3%AE-tocopherol-and-l-ascorbic-acid
#7
Vessela D Kancheva, Adriana K Slavova-Kazakova, Silvia E Angelova, Prashant Kumar, Shashwat Malhotra, Brajendra K Singh, Luciano Saso, Ashok K Prasad, Virinder S Parmar
BACKGROUND: Coumarin derivatives possess a wide range of biological activities. By functionalization of the parent coumarin skeleton that has neither antioxidant nor biological activity a series of new bio-antioxidants has been designed. RESULTS: New antioxidant compositions (equimolar binary and ternary mixtures) of eight 4-methylcoumarins and three related compounds have been tested and different effects between the individual components have been observed: positive effect (synergism), summary effect (additivism) and negative effect (antagonism)...
January 18, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29344535/sexual-experience-of-iranian-women-in-their-middle-life-a-qualitative-approach
#8
Sedigheh Moghasemi, Giti Ozgoli, Fazlollah Ahmadi, Masomeh Simbar
Background: Sexual problems are common among the middle-aged women; however, there is no deep understanding of sexuality in midlife. The current study aimed to investigate Iranian women's attitudes and experiences about sexual life changes in midlife. Methods: This is a descriptive qualitative study. Seventeen women aged 40 -65 years old were purposively selected from urban health centers in Gorgan, Iran, in 2015. Face-to-face, semi-structured and in-depth interviews were conducted for data collection until data saturation was attained...
January 2018: International Journal of Community Based Nursing and Midwifery
https://www.readbyqxmd.com/read/29344419/dendrobium-sources-of-active-ingredients-to-treat-age-related-pathologies
#9
REVIEW
Veronika Cakova, Frederic Bonte, Annelise Lobstein
Dendrobium represents one of the most important orchid genera, ornamentally and medicinally. Dendrobiums are sympodial epiphytic plants, which is a name they are worthy of, the name coming from Greek origin: "dendros", tree, and "bios", life. Dendrobium species have been used for a thousand years as first-rate herbs in traditional Chinese medicine (TCM). They are source of tonic, astringent, analgesic, antipyretic, and anti-inflammatory substances, and have been traditionally used as medicinal herbs in the treatment of a variety of disorders, such as, nourishing the stomach, enhancing production of body fluids or nourishing Yin...
December 2017: Aging and Disease
https://www.readbyqxmd.com/read/29343864/electrochemical-and-friction-characteristics-of-metallic-glass-composites-at-the-microstructural-length-scales
#10
Aditya Ayyagari, Vahid Hasannaeimi, Harpreet Arora, Sundeep Mukherjee
Metallic glass composites represent a unique alloy design strategy comprising of in situ crystalline dendrites in an amorphous matrix to achieve damage tolerance unseen in conventional structural materials. They are promising for a range of advanced applications including spacecraft gears, high-performance sporting goods and bio-implants, all of which demand high surface degradation resistance. Here, we evaluated the phase-specific electrochemical and friction characteristics of a Zr-based metallic glass composite, Zr56...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343818/a-surfactant-polymer-dressing-potentiates-antimicrobial-efficacy-in-biofilm-disruption
#11
Piya Das Ghatak, Shomita S Mathew-Steiner, Priyanka Pandey, Sashwati Roy, Chandan K Sen
A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders proper wound closure. The objective of this study was to characterize the mechanism of action of SPD inhibition of bacterial biofilm development. Static biofilms (48 h) of the primary wound pathogens Pseudomonas aeruginosa (PA01), Staphylococcus aureus (USA300) were grown on polycarbonate membranes and treated with SPD with and without antibiotics for an additional 24 h...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343724/the-uv-filtering-potential-of-drop-casted-layers-of-frustules-of-three-diatom-species
#12
Yanyan Su, Torben A Lenau, Emil Gundersen, Jacob J K Kirkensgaard, Christian Maibohm, Jérôme Pinti, Marianne Ellegaard
Diatoms are in focus as biological materials for a range of photonic applications. Many of these applications would require embedding a multitude of diatoms in a matrix (e.g. paint, crème or lacquer); however, most studies on the photonic and spectral properties of diatoms frustules (silica walls) have been carried out on single cells. In this study, for the first time, we test the spectral properties of layers of frustules of three diatom species (Coscinodiscus granii, Thalassiosira punctifera and Thalassiosira pseudonana), with special focus on transmission and reflectance in the UV range...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343532/clinical-laboratory-practice-recommendations-for-the-use-of-cardiac-troponin-in-acute-coronary-syndrome-expert-opinion-from-the-academy-of-the-american-association-for-clinical-chemistry-and-the-task-force-on-clinical-applications-of-cardiac-bio-markers-of
#13
Alan H B Wu, Robert H Christenson, Dina N Greene, Allan S Jaffe, Peter A Kavsak, Jordi Ordonez-Llanos, Fred S Apple
This document is an essential companion to the third iteration of the National Academy of Clinical Biochemistry [NACB,8 now the American Association for Clinical Chemistry (AACC) Academy] Laboratory Medicine Practice Guidelines (LMPG) on cardiac markers. The expert consensus recommendations were drafted in collaboration with the International Federation of Clinical Chemistry and Laboratory Medicine Task Force on Clinical Applications of Bio-Markers (IFCC TF-CB). We determined that there is sufficient clinical guidance on the use of cardiac troponin (cTn) testing from clinical practice groups...
January 17, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29342953/review-of-recent-metamaterial-microfluidic-sensors
#14
REVIEW
Ahmed Salim, Sungjoon Lim
Metamaterial elements/arrays exhibit a sensitive response to fluids yet with a small footprint, therefore, they have been an attractive choice to realize various sensing devices when integrated with microfluidic technology. Micro-channels made from inexpensive biocompatible materials avoid any contamination from environment and require only microliter-nanoliter sample for sensing. Simple design, easy fabrication process, light weight prototype, and instant measurements are advantages as compared to conventional (optical, electrochemical and biological) sensing systems...
January 15, 2018: Sensors
https://www.readbyqxmd.com/read/29341302/twisted-charge-transfer-antennas-for-ultra-bright-terbium-iii-and-dysprosium-iii-bioprobes
#15
Anh Thy Bui, Amandine Roux, Alexei Grichine, Alain Duperray, Chantal Andraud, Olivier Maury
The design of original twisted charge transfer antennas where a non planar geometry is enforced thanks to one or two bulky ortho-Me substituents allows us to prepare the corresponding ultra-bright Tb(III) and Dy(III) bioprobes. The brightness of the Tb(III) derivative compares well with that of the benchmark Tb-Lumi4 complex. The first bio-imaging experiments with a Dy(III) luminescent bioprobe are also reported.
January 17, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29340956/semi-targeted-analysis-of-complex-matrices-by-esi-ft-icr-ms-or-how-an-experimental-bias-may-be-used-as-an-analytical-tool
#16
Jasmine Hertzog, Vincent Carré, Anthony Dufour, Frédéric Aubriet
Ammonia is well suited to favor deprotonation process in electrospray ionization mass spectrometry (ESI-MS) to increase the formation of [M - H]-. Nevertheless, NH3 may react with carbonyl compounds (aldehyde, ketone) and bias the composition description of the investigated sample. This is of significant importance in the study of complex mixture such as oil or bio-oil. To assess the ability of primary amines to form imines with carbonyl compounds during the ESI-MS process, two aldehydes (vanillin and cinnamaldehyde) and two ketones (butyrophenone and trihydroxyacetophenone) have been infused in an ESI source with ammonia and two different amines (aniline and 3-chloronaniline)...
January 16, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29340698/simultaneous-detection-of-chicken-cytokines-in-plasma-samples-using-the-bio-plex-assay
#17
Jitka Mucksová, Karel Chalupský, Jirí Plachý, Jirí Kalina, Pavla Rachacová, Ondrej Stanek, Pavel Trefil
A chicken multiplex cytokine assay (Bio-Plex) to detect four different cytokines (IL-2, IL-12, IL-10, and interferon gamma) simultaneously in plasma samples was designed. Most standard curves range between 1 to 5 pg/mL and 5,000 pg/mL, except for IFNγ with the range of 50 to 25,000 pg/mL. Such a chicken multiplex assay proved to be fast and reliable, and comparable in sensitivity, accuracy, and reproducibility to conventional enzyme-linked immunosorbent assays. Comparison of the multiplex assay with the ELISA technique using the same clones of detection and capture antibodies resulted in correlation coefficients for all cytokines ranging from 0...
January 11, 2018: Poultry Science
https://www.readbyqxmd.com/read/29340446/-tensions-and-contradictions-in-government-interventions-for-the-promotion-of-breastfeeding
#18
Rosa María Ramos Rodríguez, María Gabriela Hernández González
With the purpose of shedding light on the decrease in the practice of breastfeeding in rural areas of Mexico, this article looks at the current biomedical model and the policies and actions to promote breastfeeding derived from the model's theoretical approach. The article also discusses operational strategies of the governmental social welfare program Oportunidades. For this purpose, the study utilizes the testimonies of 39 young breastfeeding mothers, 11 mothers and grandmothers and 12 members of the health staff in the Nahuatl population of Cuentepec, Morelos, Mexico, which were collected during a previous study in 2008 and 2009...
October 2017: Salud Colectiva
https://www.readbyqxmd.com/read/29340401/a-nanopaper-based-artificial-tongue-a-ratiometric-fluorescent-sensor-array-on-bacterial-nanocellulose-for-chemical-discrimination-applications
#19
Samira Abbasi-Moayed, Hamed Golmohammadi, M Reza Hormozi-Nezhad
In the present study, a ratiometric fluorescent sensor array as an artificial tongue has been developed on a nanopaper platform for chemical discrimination applications. The bacterial cellulose (BC) nanopaper was utilized for the first time as a novel, flexible, and transparent substrate in the optical sensor arrays for developing high-performance artificial tongues. To fabricate this platform, the hydrophobic walls on the BC nanopaper substrates were successfully created using a laser printing technology. In addition, we have used the interesting photoluminescence (PL) properties of an immobilized ratiometric probe (carbon dot-Rhodamine B (CD-RhB) nanohybrids) on the nanopaper platform to improve the visual discrimination analysis...
January 17, 2018: Nanoscale
https://www.readbyqxmd.com/read/29339969/a-new-toolset-for-protein-expression-and-subcellular-localization-studies-in-citrus-and-its-application-to-citrus-tristeza-virus-proteins
#20
Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin, Vladimir Orbovic
Background: Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work efficiently with many gene constructs, and there is a need for a more robust transient expression system in citrus leaves. Biolistic particle delivery is an alternative to Agrobacterium transformation, and in some plants, such as Arabidopsis, gives higher transformation rates in leaf tissues than Agrobacterium...
2018: Plant Methods
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