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Dientamoeba fragilis

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https://www.readbyqxmd.com/read/28725935/erratum-to-the-role-of-blastocystis-sp-and-dientamoeba-fragilis-in-irritable-bowel-syndrome-a-systematic-review-and-meta-analysis
#1
Ali Rostami, Seyed Mohammad Riahi, Ali Haghighi, Vafa Saber, Bahram Armon, Seyyed Javad Seyyedtabaei
No abstract text is available yet for this article.
July 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28697994/molecular-epidemiology-of-dientamoeba-fragilis
#2
REVIEW
Simone M Cacciò
The flagellated protozoan Dientamoeba fragilis is one of the most commonly diagnosed parasite of the human gut, with a global distribution. Nevertheless, essential aspects of its biology remain incompletely understood or controversial, most notably life cycle, host range, transmission routes and the ability to cause disease. Molecular epidemiologic studies are also scarce, and limited by the lack of informative genotyping tools. To date, two D. fragilis genotypes (1 and 2) are recognized, with a strong predominance of genotype 1 in both humans and few animal hosts...
July 8, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28668983/the-role-of-blastocystis-sp-and-dientamoeba-fragilis-in-irritable-bowel-syndrome-a-systematic-review-and-meta-analysis
#3
REVIEW
Ali Rostami, Seyed Mohammad Riahi, Ali Haghighi, Vafa Saber, Bahram Armon, Seyyed Javad Seyyedtabaei
Irritable bowel syndrome (IBS) is globally one of the most prevalent gastrointestinal disorders with a negative impact on quality of life and socio-economic status of patients. Recently, controversial evidences suggest that Blastocystis sp. and Dientamoeba fragilis infections may be implicated in the development of IBS. We performed a systematic review and meta-analysis to examine the possible association regarding this issue. PubMed, ScienceDirect, Scopus, Web of Science, and Cochrane electronic databases were searched (up to February 2017) to identify the relevant studies...
July 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28648295/detection-and-prevalence-of-protozoan-parasites-in-ready-to-eat-packaged-salads-on-sale-in-italy
#4
Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero
To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in 'ready to eat' (RTE) salads on sale in Italy, 648 packages were purchased from industrial and local brands. Nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. Microscopic examination of 864 slides detected Cryptosporidium spp. but also Blastocystis hominis and Dientamoeba fragilis. Molecular tools identified G...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28603232/prevalence-of-intestinal-parasitoses-detected-in-padua-teaching-hospital-italy-march-2011-february-2013
#5
Andrea Bartolini, Giulia Zorzi, Valeria Besutti
The aim of this study was to evaluate the distribution of parasitic intestinal infections in patients attending Padua teaching hospital during a two-year period. Between 1st March 2011 and 28th February 2013, we examined stool specimens from 7341 patients (6127 Italians, 1214 non-Italians) for ova and parasites using microscopy, rapid enzyme immunoassays, culture techniques and molecular methods. Stools of 1080 patients (14.71%) were positive for parasites; a total of 1349 intestinal parasites were counted...
June 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/28458199/occurrence-and-removal-efficiency-of-parasitic-protozoa-in-swedish-wastewater-treatment-plants
#6
Björn Berglund, Olaf Dienus, Ekaterina Sokolova, Emma Berglind, Andreas Matussek, Thomas Pettersson, Per-Eric Lindgren
Giardia intestinalis, Cryptosporidium spp., Entamoeba histolytica and Dientamoeba fragilis are parasitic protozoa and causative agents of gastroenteritis in humans. G. intestinalis and Cryptosporidium spp. in particular are the most common protozoa associated with waterborne outbreaks in high-income countries. Surveillance of protozoan prevalence in wastewater and evaluation of wastewater treatment removal efficiencies of protozoan pathogens is therefore imperative for assessment of human health risk. In this study, influent and effluent wastewater samples from three wastewater treatment plants in Sweden were collected over nearly one year and assessed for prevalence of parasitic protozoa...
November 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28330885/dientamoeba-fragilis-a-commensal-in-children-in-danish-day-care-centers
#7
Pikka Jokelainen, Betina Hebbelstrup Jensen, Bente Utoft Andreassen, Andreas Munk Petersen, Dennis Röser, Karen A Krogfelt, Henrik V Nielsen, Christen R Stensvold
Dientamoeba fragilis is an intestinal protozoan of debated clinical significance. Here, we present cross-sectional and longitudinal observations on D. fragilis in children aged 0 to 6 years from a 1-year multi-day-care-center cohort study set in Copenhagen, Denmark. The inclusion period for the cohort was 2009 through 2012. Stool samples collected from the children were accompanied by questionnaires completed by the parents or guardians of the children. Using real-time PCR, D. fragilis was detected in the first stool sample from 97 of 142 (68...
June 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28290266/improved-diagnosis-of-trichuris-trichiura-by-using-a-bead-beating-procedure-on-ethanol-preserved-stool-samples-prior-to-dna-isolation-and-the-performance-of-multiplex-real-time-pcr-for-intestinal-parasites
#8
Maria M M Kaisar, Eric A T Brienen, Yenny Djuardi, Erliyani Sartono, Maria Yazdanbakhsh, Jaco J Verweij, Taniawati Supali, Lisette VAN Lieshout
For the majority of intestinal parasites, real-time PCR-based diagnosis outperforms microscopy. However, the data for Trichuris trichiura have been less convincing and most comparative studies have been performed in populations with low prevalence. This study aims to improve detection of T. trichuria DNA in human stool by evaluating four sample preparation methods. Faecal samples (n = 60) were collected at Flores island, Indonesia and examined by microscopy. Aliquots were taken and a bead-beating procedure was used both on directly frozen stool and on material preserved with 96% ethanol...
June 2017: Parasitology
https://www.readbyqxmd.com/read/28007484/diagnosis-of-intestinal-parasites-in-a-rural-community-of-venezuela-advantages-and-disadvantages-of-using-microscopy-or-rt-pcr
#9
COMPARATIVE STUDY
Renzo Nino Incani, Elizabeth Ferrer, Denise Hoek, Robbert Ramak, Jeroen Roelfsema, Lapo Mughini-Gras, Titia Kortbeek, Elena Pinelli
A cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time PCR (RT-PCR) for 14 intestinal parasites in a Venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. A total of 228 participants were included in this study. A multiplex RT-PCR was used for the detection of Dientamoeba fragilis, Giardia intestinalis, Cryptosporidium sp. and a monoplex RT-PCR for Entamoeba histolytica...
March 2017: Acta Tropica
https://www.readbyqxmd.com/read/27861635/multiplex-real-time-pcr-assay-targeting-eight-parasites-customized-to-the-korean-population-potential-use-for-detection-in-diarrheal-stool-samples-from-gastroenteritis-patients
#10
Eun Jeong Won, Soo Hyun Kim, Seung Jung Kee, Jong Hee Shin, Soon Pal Suh, Jong Yil Chai, Dong Wook Ryang, Myung Geun Shin
Intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. We developed a multiplex real-time PCR assay for detection of eight intestinal parasites and prospectively evaluated it for patients with gastroenteritis. The assay targeted Cryptosporidium parvum, Giardia lamblia, Entamoeba histolytica, Blastocystis hominis, Dientamoeba fragilis, Clonorchis sinensis, Metagonimus yokogawai, and Gymnophalloides seoi...
2016: PloS One
https://www.readbyqxmd.com/read/27784723/dientamoeba-fragilis-colonization-is-not-associated-with-gastrointestinal-symptoms-in-children-at-primary-care-level
#11
Gea A Holtman, Justin J Kranenberg, Marco H Blanker, Alewijn Ott, Yvonne Lisman-van Leeuwen, Marjolein Y Berger
BACKGROUND: Dientamoeba fragilis is commonly identified in children in primary care and is suspected to cause gastrointestinal disease. OBJECTIVE: To determine the association between D. fragilis colonization and gastrointestinal symptoms in children. METHODS: We performed a cross-sectional study with children who presented in primary care with gastrointestinal symptoms. The associations between D. fragilis colonization and specific symptoms were explored by means of logistic regression analyses...
October 26, 2016: Family Practice
https://www.readbyqxmd.com/read/27555056/multilocus-sequence-typing-of-dientamoeba-fragilis-identified-a-major-clone-with-widespread-geographical-distribution
#12
Simone M Cacciò, Anna Rosa Sannella, Antonella Bruno, Christen R Stensvold, Erica Boarato David, Semiramis Guimarães, Elisabetta Manuali, Chiara Magistrali, Karim Mahdad, Miles Beaman, Roberta Maserati, Fabio Tosini, Edoardo Pozio
The flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown...
November 2016: International Journal for Parasitology
https://www.readbyqxmd.com/read/27523936/detection-of-dientamoeba-fragilis-in-animal-faeces-using-species-specific-real-time-pcr-assay
#13
Douglas Chan, Joel Barratt, Tamalee Roberts, Owen Phillips, Jan Šlapeta, Una Ryan, Deborah Marriott, John Harkness, John Ellis, Damien Stark
Dientamoeba fragilis is a potentially pathogenic, enteric, protozoan parasite with a worldwide distribution. While clinical case reports and prevalence studies appear regularly in the scientific literature, little attention has been paid to this parasite's biology, life cycle, host range, and possible transmission routes. Overall, these aspects of Dientamoeba biology remain poorly understood at best. In this study, a total of 420 animal samples, collected from Australia, were surveyed for the presence of Dientamoeba fragilis using PCR...
August 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27447173/evaluation-of-the-nanochip%C3%A2-gastrointestinal-panel-gip-test-for-simultaneous-detection-of-parasitic-and-bacterial-enteric-pathogens-in-fecal-specimens
#14
Shifra Ken Dror, Elsa Pavlotzky, Mira Barak
Infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. Rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. Current laboratory stool testing has a long turnaround time (TAT) and demands highly qualified personnel and multiple techniques. The need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising performance requirements...
2016: PloS One
https://www.readbyqxmd.com/read/27409092/irritable-bowel-syndrome-and-intestinal-parasites-a-view-from-south-america
#15
REVIEW
George Vasquez-Rios, Jorge D Machicado, Angelica Terashima, Luis A Marcos
Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder of uncertain etiology. Several studies have proposed the possible role of intestinal parasites in the pathogenesis of IBS. We aimed to summarize the epidemiological studies that describe a possible link between intestinal parasites and IBS, with special interest in endemic areas for intestinal parasitism such as South America. A comprehensive review of the literature was conducted by using the keywords: irritable bowel syndrome, intestinal parasites, protozoan infection, soil-transmitted helminths and South America...
April 2016: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/27372926/potential-causative-agents-of-acute-gastroenteritis-in-households-with-preschool-children-prevalence-risk-factors-clinical-relevance-and-household-transmission
#16
M Heusinkveld, L Mughini-Gras, R Pijnacker, H Vennema, R Scholts, K W van Huisstede-Vlaanderen, T Kortbeek, M Kooistra-Smid, W van Pelt
Acute gastroenteritis (AGE) morbidity remains high amongst preschool children, posing a significant societal burden. Empirical data on AGE-causing agents is needed to gauge their clinical relevance and identify agent-specific targets for control. We assessed the prevalence, risk factors and association with symptoms for enteropathogens in households with preschool children. A monthly-repeated cross-sectional survey of enteropathogens in households with preschool children was performed. A parent-child pair per household (n = 907 households) provided faecal samples and reported their symptoms and potential risk exposures...
October 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27356981/high-occurrence-of-blastocystis-sp-subtypes-1-3-and-giardia-intestinalis-assemblage-b-among-patients-in-zanzibar-tanzania
#17
Joakim Forsell, Margareta Granlund, Linn Samuelsson, Satu Koskiniemi, Helén Edebro, Birgitta Evengård
BACKGROUND: Blastocystis is a common intestinal parasite with worldwide distribution but the distribution of Blastocystis and its subtypes in East Africa is largely unknown. In this study, we investigate the distribution of Blastocystis subtypes in Zanzibar, Tanzania and report the prevalence of intestinal parasites using both molecular methods and microscopy. METHODS: Stool samples were collected from both diarrhoeic and non-diarrhoeic outpatients in Zanzibar. In addition to microscopy, real-time PCR for Blastocystis, Entamoeba histolytica and E...
June 29, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27322863/detection-of-parasites-in-children-with-chronic-diarrhea
#18
Salih Maçin, Filiz Kaya, Deniz Çağdaş, Hayriye Hizarcioglu-Gulsen, Inci Nur Saltik-Temizel, İlhan Tezcan, Hülya Demir, Sibel Ergüven, Yakut Akyön
The aim of this study was to investigate the frequency of intestinal parasites in patients with chronic diarrhea and clarify the importance of these parasitic pathogens in such cases. A total of 60 pediatric patients with chronic diarrhea between June 2012 and October 2014 were enrolled in the study. Out of 60 stool samples, five were positive for Giardia lamblia, two, Dientamoeba fragilis, and one, Blastocystis hominis. One stool sample was positive for Entamoeba hartmanni and B. hominis, another one was positive for G...
June 2016: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/27263118/is-the-treatment-of-enterobius-vermicularis-co-infection-necessary-to-eradicate-dientamoeba-fragilis-infection
#19
José A Boga, Susana Rojo, Jonathan Fernández, Mercedes Rodríguez, Carmen Iglesias, Pablo Martínez-Camblor, Fernando Vázquez, Azucena Rodríguez-Guardado
OBJECTIVES: Dientamoeba fragilis is a pathogenic protozoan of the human gastrointestinal tract with a worldwide distribution, which has emerged as an important and misdiagnosed cause of chronic gastrointestinal illnesses such as diarrhea and 'irritable-bowel-like' gastrointestinal disease. Very little research has been conducted on the use of suitable antimicrobial compounds. Furthermore, higher rates of co-infection with Enterobius vermicularis have been described, suggesting that E...
August 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27230509/associations-between-common-intestinal-parasites-and-bacteria-in-humans-as-revealed-by-qpcr
#20
L O'Brien Andersen, A B Karim, H M Roager, L K Vigsnæs, K A Krogfelt, T R Licht, C R Stensvold
Several studies have shown associations between groups of intestinal bacterial or specific ratios between bacterial groups and various disease traits. Meanwhile, little is known about interactions and associations between eukaryotic and prokaryotic microorganisms in the human gut. In this work, we set out to investigate potential associations between common single-celled parasites such as Blastocystis spp. and Dientamoeba fragilis and intestinal bacteria. Stool DNA from patients with intestinal symptoms were selected based on being Blastocystis spp...
September 2016: European Journal of Clinical Microbiology & Infectious Diseases
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