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Dientamoeba fragilis

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https://www.readbyqxmd.com/read/29762365/multiplex-real-time-pcr-detection-of-intestinal-protozoa-in-hiv-infected-children-in-malawi-enterocytozoon-bieneusi-is-common-and-associated-with-gastrointestinal-complaints-and-may-delay-bmi-nutritional-status-recovery
#1
Minke H W Huibers, Peter Moons, Nelson Maseko, Monfort B Gushu, Oluwadamilola H Iwajomo, Robert S Heyderman, Michael Boele van Hensbroek, Eric A Brienen, Lisette van Lieshout, Job C J Calis
BACKGROUND: Intestinal protozoa are common opportunistic infections in HIV patients. Longitudinal studies on either the clinical relevance, or the effect of immune reconstitution by anti-retroviral therapy on intestinal protozoan infections are children is lacking however. This study investigates prevalence and clinical relevance of intestinal protozoa in HIV-infected Malawian children prior to and during their first year of ART. METHODS: Stool samples collected at enrolment and during follow-up were tested for non-opportunistic (Giardia lamblia, Dientamoeba fragilis, Entamoeba histolytica) and opportunistic protozoa (Entroctytoon bieneusi, Encephalitozoon spp...
May 14, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29587846/evaluation-of-two-dna-extraction-methods-for-the-pcr-based-detection-of-eukaryotic-enteric-pathogens-in-fecal-samples
#2
Estelle Menu, Charles Mary, Isabelle Toga, Didier Raoult, Stéphane Ranque, Fadi Bittar
OBJECTIVE: Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the routine clinical laboratory diagnosis of eukaryotic enteric pathogens, we compare, via quantitative PCR cycle threshold (Ct) values, the efficiency of two DNA extraction kits: the semi-automated EZ1® (Qiagen) and the manual QIAamp® DNA Stool Mini Kit (Qiagen), on six protozoa: Blastocystis spp., Cryptosporidium parvum/hominis, Cyclospora cayetanensis, Dientamoeba fragilis, Giardia intestinalis and Cystoisospora belli and one microsporidia: Enterocytozoon bieneusi...
March 27, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29581055/evaluation-of-the-roche-lightmix-%C3%A2-gastro-parasites-multiplex-pcr-assay-detecting-giardia-duodenalis-entamoeba-histolytica-cryptosporidia-dientamoeba-fragilis-and-blastocystis-hominis
#3
Johannes Friesen, Jörg Fuhrmann, Heike Kietzmann, Egbert Tannich, Michael Müller, Ralf Ignatius
OBJECTIVES: Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix® Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. METHODS: Stool specimens (n=1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen® enzyme immunoassays (EIAs) detecting G...
March 23, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29503786/a-mexican-honeymoon-marred-by-gastrointestinal-upset-a-case-of-dientamoeba-fragilis-causing-post-infectious-irritable-bowel-syndrome
#4
Saeed Ali, Neelam Khetpal, Muhammad Talha Khan, Mamoon Rasheed, Fnu Asad-Ur-Rahman, Karen Echeverria-Beltran
Dientamoeba fragilis (D. fragilis) is an anaerobic intestinal protozoan parasite that has been associated with irritable bowel syndrome (IBS)-like symptoms. We report a case of post-infectious IBS caused by D. fragilis treated successfully with metronidazole. A 33-year-old African American male with an unremarkable past medical history was seen in the office with a three-month history of intermittent, generalized, crampy abdominal pain with bloating and flatulence without associated weight loss. He visited Mexico for his honeymoon four months ago...
December 27, 2017: Curēus
https://www.readbyqxmd.com/read/29451859/harmonization-of-pcr-based-detection-of-intestinal-pathogens-experiences-from-the-dutch-external-quality-assessment-scheme-on-molecular-diagnosis-of-protozoa-in-stool-samples
#5
Theo A Schuurs, Rob Koelewijn, Eric A T Brienen, Titia Kortbeek, Theo G Mank, Bert Mulder, Foekje F Stelma, Lisette van Lieshout, Jaap J van Hellemond
BACKGROUND: Real-time PCR methods are increasingly used in routine patient care settings not only to determine the presence or absence of pathogens in patient materials, but also to obtain semiquantitative results to estimate the pathogen load. However, it is so far unknown how well these methods are harmonized among different laboratories. METHODS: Sets of stool samples were distributed three to four times per year to ca. 25-40 participating laboratories within the European Union as part of an external quality assessment scheme (EQAS) for the detection of gastrointestinal protozoa...
February 16, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29404747/does-dientamoeba-fragilis-cause-diarrhea-a-systematic-review
#6
Zing-Wae Wong, Kate Faulder, Joan L Robinson
It remains controversial whether Dientamoeba fragilis is a commensal parasite or a pathogen. The objective of this systematic review was to establish the strength of the evidence that Dientamoeba fragilis would cause diarrhea. A search was performed for studies that reported either the association between D. fragilis detection in stools and diarrhea or diarrhea outcomes with D. fragilis therapy or challenge. Data from seven studies of specific populations reported that 22% had D. fragilis in stools of which only 23% had diarrhea...
April 2018: Parasitology Research
https://www.readbyqxmd.com/read/29318125/prevalence-of-intestinal-parasites-in-food-handlers-of-bandar-abbas-southern-iran
#7
Mehrgan Heydari-Hengami, Yaghoob Hamedi, Majid Najafi-Asl, Khojasteh Sharifi-Sarasiabi
Background: Food handlers play a significant role in the dissemination and transmission of enteropathogenic microorganisms, including intestinal parasites. The aim of this study was to determine the prevalence of intestinal parasites in food handlers of Bandar Abbas, southern Iran. Methods: In this cross-sectional study, 800 stool samples were randomly collected in a period of 6 months from Jun to Nov 2015. The diagnosis was made on direct wet mount, formalin-ether concentration, Ziehl-Neelsen, and Trichrome stained slides...
January 2018: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/29301570/maldi-tof-ms-as-a-new-tool-for-the-identification-of-dientamoeba-fragilis
#8
Adriana Calderaro, Mirko Buttrini, Sara Montecchini, Sabina Rossi, Giovanna Piccolo, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto
BACKGROUND: In this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spectrum and 14 clinical isolates to verify the reliability of the obtained results. RESULTS: While 15 peaks were found to be discriminating between the reference strain and the culture medium used, six peaks, observed in all the 14 strains tested, were considered as markers able to identify D...
January 4, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29237648/parasitic-stool-testing-in-newly-arrived-refugees-in-calgary-alta
#9
Giselle DeVetten, Meghan Dirksen, Robert Weaver, Tanvir Turin Chowdhury, Michael William Aucoin
OBJECTIVE: To determine the prevalence of intestinal parasites and rates of stool testing compliance, as well as associated patient characteristics, among newly arrived refugees at the Mosaic Refugee Health Clinic in Calgary, Alta. DESIGN: Retrospective chart review. SETTING: Primary care clinic for refugee patients. PARTICIPANTS: A total of 1390 new refugee patients at the clinic from May 1, 2011, to June 30, 2013. MAIN OUTCOME MEASURES: Stool ova and parasite test completion and proportion of positive test results...
December 2017: Canadian Family Physician Médecin de Famille Canadien
https://www.readbyqxmd.com/read/29078992/prevalence-of-d-fragilis-infection-in-the-household-contacts-of-a-group-of-infected-patients
#10
Candela Menéndez Fernández-Miranda, Jonathan Fernández-Suarez, Mercedes Rodríguez-Pérez, Pablo Menéndez Fernández-Miranda, Fernando Vázquez, Jose A Boga Ribeiro, Azucena Rodríguez-Guardado
INTRODUCTION: The results of a study on the household contacts of patients with D. fragilis infection are presented. METHODS: A prospective, descriptive study was carried out on all Dientamoeba fragilis-infected patients treated at the Tropical Medicine Unit of Hospital Universitario Central de Asturias between 2012- 2017 and their household contacts. Three stool samples per patient and three stool samples from each of their household contacts were concentrated and analysed...
October 24, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29055284/multiple-sampling-and-saf-fixative-triple-faeces-testing-for-dysbiosis-and-pathogenic-infections-of-the-gastrointestinal-tract-case-report
#11
Johanna Mauss, Michelle Retz, Roger Dilts, Jeffrey Langland
The classic presentation of acute gastrointestinal (GI) parasitic infection including fever, diarrhea, nausea, and/or abdominal pain is not always a reliable measure. In these cases, parasitic infections require sensitive stool testing to be identified and treated effectively. The standard practice for stool analysis typically requires 3 separate stool specimens to be collected on 3 separate days to overcome the possibility of misdiagnosis due to intermittent shedding. Nonetheless, this practice is not often utilized due to very short periods of sample viability, which often leads to patient misdiagnosis...
October 21, 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28979346/prevalence-and-risk-factors-of-human-intestinal-parasites-in-roudehen-tehran-province-iran
#12
Nasrin Hemmati, Elham Razmjou, Saeideh Hashemi-Hafshejani, Abbas Motevalian, Lameh Akhlaghi, Ahmad Reza Meamar
BACKGROUND: Intestinal parasitic infections are among the most common infections and health problems worldwide. Due to the lack of epidemiologic information of such infections, the prevalence of, and the risk factors for, enteric parasites were investigated in residents of Roudehen, Tehran Province, Iran. METHODS: In this cross-sectional study, 561 triple fecal samples were collected through a two-stage cluster-sampling protocol from Jun to Dec 2014. The samples were examined by formalin-ether concentration, culture, and with molecular methods...
July 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28957005/systemic-histomoniasis-in-a-leucistic-indian-peafowl-pavo-cristatus-from-southern-brazil
#13
Mariana de Mello Zanim Michelazzo, João Pedro Sasse, Marielen de Souza, Victor Hugo Brunaldi Marutani, Ana Angelita Sampaio Baptista, João Luis Garcia, Amauri Alcindo Alfieri, Selwyn Arlington Headley
The pathological and molecular findings associated with Histomonas meleagridis are described in a leucistic Indian peafowl (Pavo cristatus) from Southern Brazil. The most significant gross findings were multifocal necrotizing hepatitis and diphtheric typhlitis. Histopathologic evaluation of the liver, ceca, kidney, spleen, and small intestine revealed systemic histomoniasis (SH) associated with intralesional and intravascular accumulations of histomonad organisms consistent with H. meleagridis. PCR was used to amplify the DNA of H...
September 2017: Avian Diseases
https://www.readbyqxmd.com/read/28880141/-clioquinol-use-for-dientamoeba-fragilis-infections-is-questionable
#14
F van Hunsel, C van Nieuwkoop, B H Ch Stricker
Clioquinol is used for treatment of amoebiasis and infection with Dientamoeba fragilis. In a guideline of the Dutch Working Party on Antibiotic Policy, clioquinol is recommended as a first-choice treatment for Dientamoeba fragilis. This drug, however, is associated with subacute myelo-optico-neuropathy (SMON). It was withdrawn from the market worldwide in 1985 by manufacturer Ciba-Geigy. Although the Dutch Medicines Evaluation Board has registered no products for systemic use of clioquinol since then, the drug is available as a pharmacy-compounded drug and the last few years the use of clioquinol in the Netherlands has risen again...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28877704/coproscopy-and-molecular-screening-for-detection-of-intestinal-protozoa
#15
Marawan Abu-Madi, Sonia Boughattas, Jerzy M Behnke, Aarti Sharma, Ahmed Ismail
BACKGROUND: Intestinal parasitosis is one of several health concerns about immigrants who travel from endemic to non-endemic regions. Reliable rapid sensitive diagnostic tools, for use in non-endemic regions, are urgently required to enable frequent assessment of immigrant workers in jobs where risk of local transmission is a particular concern (e.g. food-handlers). We assessed the burden of intestinal protozoa in newly arrived immigrants and those applying for renewal of work permits in Qatar (n = 735), by both microscopic examination of stool samples and by Real Time PCR methodology...
September 6, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28842214/travel-related-acquisition-of-diarrhoeagenic-bacteria-enteral-viruses-and-parasites-in-a-prospective-cohort-of-98-dutch-travellers
#16
Jarne M van Hattem, Maris S Arcilla, Martin P Grobusch, Aldert Bart, Martin C Bootsma, Perry J van Genderen, Tom van Gool, Abraham Goorhuis, Jaap J van Hellemond, Richard Molenkamp, Nicky Molhoek, Astrid M Oude Lashof, Ellen E Stobberingh, Bob de Wever, Henri A Verbrugh, Damian C Melles, John Penders, Constance Schultsz, Menno D de Jong
BACKGROUND: Limited prospective data are available on the acquisition of viral, bacterial and parasitic diarrhoeagenic agents by healthy individuals during travel. METHODS: To determine the frequency of travel associated acquisition of 19 pathogens in 98 intercontinental travellers, qPCR was used to detect 8 viral pathogens, 6 bacterial enteric pathogens and 5 parasite species in faecal samples collected immediately before and after travel. RESULTS: We found high pre-travel carriage rates of Blastocystis spp...
August 24, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/28725935/erratum-to-the-role-of-blastocystis-sp-and-dientamoeba-fragilis-in-irritable-bowel-syndrome-a-systematic-review-and-meta-analysis
#17
Ali Rostami, Seyed Mohammad Riahi, Ali Haghighi, Vafa Saber, Bahram Armon, Seyyed Javad Seyyedtabaei
No abstract text is available yet for this article.
September 2017: Parasitology Research
https://www.readbyqxmd.com/read/28697994/molecular-epidemiology-of-dientamoeba-fragilis
#18
REVIEW
Simone M Cacciò
The flagellated protozoan Dientamoeba fragilis is one of the most commonly diagnosed parasite of the human gut, with a global distribution. Nevertheless, essential aspects of its biology remain incompletely understood or controversial, most notably life cycle, host range, transmission routes and the ability to cause disease. Molecular epidemiologic studies are also scarce, and limited by the lack of informative genotyping tools. To date, two D. fragilis genotypes (1 and 2) are recognized, with a strong predominance of genotype 1 in both humans and few animal hosts...
July 8, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28668983/the-role-of-blastocystis-sp-and-dientamoeba-fragilis-in-irritable-bowel-syndrome-a-systematic-review-and-meta-analysis
#19
REVIEW
Ali Rostami, Seyed Mohammad Riahi, Ali Haghighi, Vafa Saber, Bahram Armon, Seyyed Javad Seyyedtabaei
Irritable bowel syndrome (IBS) is globally one of the most prevalent gastrointestinal disorders with a negative impact on quality of life and socio-economic status of patients. Recently, controversial evidences suggest that Blastocystis sp. and Dientamoeba fragilis infections may be implicated in the development of IBS. We performed a systematic review and meta-analysis to examine the possible association regarding this issue. PubMed, ScienceDirect, Scopus, Web of Science, and Cochrane electronic databases were searched (up to February 2017) to identify the relevant studies...
September 2017: Parasitology Research
https://www.readbyqxmd.com/read/28648295/detection-and-prevalence-of-protozoan-parasites-in-ready-to-eat-packaged-salads-on-sale-in-italy
#20
Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero
To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in 'ready to eat' (RTE) salads on sale in Italy, 648 packages were purchased from industrial and local brands. Nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. Microscopic examination of 864 slides detected Cryptosporidium spp. but also Blastocystis hominis and Dientamoeba fragilis. Molecular tools identified G...
October 2017: Food Microbiology
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