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https://www.readbyqxmd.com/read/27914366/rapid-and-low-cost-biosensor-for-the-detection-of-staphylococcus-aureus
#1
Ghadeer A R Y Suaifan, Sahar Alhogail, Mohammed Zourob
Staphylococcus aureus (S. aureus) is one of the most common etiological agents in hospital-acquired infections and food-borne illness. S. aureus toxins and virulence proteases often circulate in host blood vessels leading to life-threatening diseases. Standard identification approaches include bacterial culturing method, which takes several days. Other nucleic acid-based methods were expensive and required trained personnel. To surmount these limitations, a paper-based biosensor was developed. The sensing mechanism was based on the proteolytic activity of S...
November 21, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27914320/biochemical-precursor-effects-on-the-fatty-acid-production-in-cell-suspension-cultures-of-theobroma-cacao-l
#2
O Parra, A M Gallego, A Urrea, L F Rojas, C Correa, L Atehortúa
Cocoa butter (CB) is composed of 96% palmitic, stearic, oleic, linoleic and linolenic fatty acids that are responsible for the hardness, texture and fusion properties of chocolate. Through in vitro plant cell culture it is possible to modify CB lipid profiles and to study the fatty acid biosynthesis pathway on a subcellular level, evaluating fundamental aspects to enhance in vitro fatty acid production in a specific and controlled way. In this research, culture media was supplemented with acetate, biotin, pyruvate, bicarbonate and glycerol at three different concentrations and the effects on the biomass production (g/L), cell viability, and fatty acids profile and production was evaluated in in vitro cell suspensions culture...
November 25, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27914303/a-necessary-evil-residential-placement-of-people-with-intellectual-disability-among-the-palestinian-minority-in-israel
#3
Kareem Nasser, Dalia Sachs, Amalia Sa'ar
Among the Palestinian minority in Israel, residential placement of people with intellectual disabilities [ID] is relatively new and steadily increasing, but poorly studied. A qualitative design was used to explore the process of residential placement decision by 18 parents of people with ID through semi-structured interviews. Sampling was purposive and data was analyzed thematically. Four main themes emerged representing parents' perceptions and experiences along the placement decision-making process, together indicating an experience of deeply 'conflicted parenting': (a) initial resistance to placement and its perception as abandonment and parental failure; (b) attrition following cumulative difficulties and lack of assistance; (c) resignation and reframing of placement as a necessary evil; (d) relief mixed with guilt, pain, and ambivalence following placement...
November 30, 2016: Research in Developmental Disabilities
https://www.readbyqxmd.com/read/27914268/anti-adhesive-antimicrobial-peptide-coating-prevents-catheter-associated-infection-in-a-mouse-urinary-infection-model
#4
Kai Yu, Joey C Y Lo, Mei Yan, Xiaoqiang Yang, Donald E Brooks, Robert E W Hancock, Dirk Lange, Jayachandran N Kizhakkedathu
Catheter-associated urinary tract infections (CAUTIs) represent one of the most common hospital acquired infections with significant economic consequences and increased patient morbidity. CAUTIs often start with pathogen adhesion and colonization on the catheter surface followed by biofilm formation. Current strategies to prevent CAUTIs are insufficiently effective and antimicrobial coatings based on antimicrobial peptides (AMPs) hold promise in curbing CAUTIs. Here we report an effective surface tethering strategy to prepare AMP coatings on polyurethane (PU), a common biomedical plastic used for catheter manufacture, by using an anti-adhesive hydrophilic polymer coating...
November 24, 2016: Biomaterials
https://www.readbyqxmd.com/read/27914266/skin-integrated-with-perfusable-vascular-channels-on-a-chip
#5
Nobuhito Mori, Yuya Morimoto, Shoji Takeuchi
This paper describes a method for fabricating perfusable vascular channels coated with endothelial cells within a cultured skin-equivalent by fixing it to a culture device connected to an external pump and tubes. A histological analysis showed that vascular channels were constructed in the skin-equivalent, which showed a conventional dermal/epidermal morphology, and the endothelial cells formed tight junctions on the vascular channel wall. The barrier function of the skin-equivalent was also confirmed. Cell distribution analysis indicated that the vascular channels supplied nutrition to the skin-equivalent...
November 27, 2016: Biomaterials
https://www.readbyqxmd.com/read/27914252/synthesis-of-copper-containing-bioactive-glass-nanoparticles-using-a-modified-st%C3%A3-ber-method-for-biomedical-applications
#6
Kai Zheng, Xinyi Dai, Miao Lu, Norbert Hüser, Nicola Taccardi, Aldo R Boccaccini
Copper (Cu)-containing bioactive glasses (BGs) are attracting attention for bone regeneration and wound healing since they have bone-bonding capability and potential osteogenesis and angiogenesis properties. In this study, highly dispersed and spherical Cu-containing bioactive glass nanoparticles (Cu-BGNs) were successfully synthesized via a modified Stöber method. The content of incorporated Cu in the particles could be tailored by adjusting the amount of the added Cu precursor, a procedure that had no significant effects on the morphological and structural characteristics of the nanoparticles...
November 15, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27914239/sixth-graders-in-israel-recount-their-experience-of-verbal-abuse-by-teachers-in-the-classroom
#7
Brenda Geiger
AIM: This study adopts a children's rights perspective stated in Articles 3, 12, 19, 28 of the Convention on the Rights of Children (1989) with the goal of listening to Israeli children articulate their experience of verbal abuse by teachers in 6th grade classrooms METHODS: A purposeful sample of sixty students was individually interviewed and content analysis was performed following Strauss' (1987) constant comparative method. RESULTS: Students reported teachers' yelling, name-calling and put-downs when failing to pay attention, complete their work, or obtain good grades...
November 30, 2016: Child Abuse & Neglect
https://www.readbyqxmd.com/read/27914213/parental-support-for-human-papilloma-virus-vaccination-by-adolescents-in-ibadan-north-local-government-area-ibadan-nigeria
#8
Magbagbeola David Dairo, Moradeke Omoyemi Adeleke, Adetokunbo Taophic Salawu, Ayobami David Adewole
BACKGROUND: Despite changing sexual norms and mores generally perceived to be permissive of sexual expression, parents continue to serve as gateways to uptake of reproductive health services and commodities in our cultural settings. However, their support for adolescent uptake of human papilloma virus (HPV) vaccine are not well explored and documented. This study aims to assess parental knowledge and awareness of HPV infection and cervical cancer and their support for HPV vaccination for their adolescent children...
December 3, 2016: International Journal of Adolescent Medicine and Health
https://www.readbyqxmd.com/read/27914202/probiotic-roles-of-lactobacillus-spp-in-swine-insights-from-gut-microbiota
#9
REVIEW
Valerie Diane V Valeriano, Marilen P Balolong, Dae-Kyung Kang
The use of lactobacilli as probiotics in swine has been gaining attention due to their ability to improve growth performance and carcass quality, prevent gastrointestinal infection, and most importantly, their 'generally recognised as safe' status. Previous studies support the potential of lactobacilli to regulate host immune systems, enhance gut metabolic capacities, and maintain balance in the gut microbiota. Research on swine gut microbiota has revealed complex gut microbial community structure and showed the importance of Lactobacillus to the host's health...
December 3, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27914190/culturally-competent-patient-provider-communication-in-the-management-of-cancer-an-integrative-literature-review
#10
REVIEW
Ottilia Brown, Wilma Ten Ham-Baloyi, Dalena Rm van Rooyen, Colleen Aldous, Leonard Charles Marais
BACKGROUND: Managing cancer in a multicultural environment poses several challenges, which include the communication between the patient and the healthcare provider. Culture is an important consideration in clinical care as it contributes to shaping patients' health-related values, beliefs, and behaviours. This integrative literature review gathered evidence on how culturally competent patient-provider communication should be delivered to patients diagnosed with cancer. DESIGN: Whittemore and Knafl's approach to conducting an integrative literature review was used...
2016: Global Health Action
https://www.readbyqxmd.com/read/27914161/streptococcus-agalactiae-native-valve-endocarditis-uncommon-presentation-of-multiple-myeloma
#11
Ana Pinho Oliveira, Anne Delgado, Cláudia Martins, Pedro Gama
Adults with chronic immunosuppressive conditions are at an increased risk for Streptococcus agalactiae endocarditis, which is typically characterized by acute onset, presence of large vegetations, rapid valvular destruction and frequent complications. We report a rare case of a 74 years old man presenting with fever, renal infarction, ischemic stroke and uveitis. Infective endocarditis was diagnosed and Streptococcus agalactiae was isolated in blood cultures. A multiple myeloma Ig G-K was also diagnosed. The infective endocarditis was successfully treated with a course of benzylpenicillin and gentamicin...
August 2016: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/27914127/impact-of-initial-vancomycin-trough-concentration-on-clinical-and-microbiological-outcomes-of-methicillin-resistant-staphylococcus-aureus-bacteremia-in-children
#12
Ree Nar Yoo, Seo Hee Kim, Jina Lee
It is important to use vancomycin in a proper manner to ensure optimal drug exposure. Despite extensive use of vancomycin in children, studies on its optimal trough concentration (Ctrough) in the pediatric population remained rare. This retrospective study included children < 18 years old with culture-confirmed methicillin-resistant Staphylococcus aureus (MRSA) bacteremia who were hospitalized in our institute from January 2010 to April 2014. Clinical characteristics, initial vancomycin dose, Ctrough and clinical/microbiological outcomes were retrospectively collected from medical records...
January 2017: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/27914106/effect-of-cranberry-extract-on-the-frequency-of-bacteriuria-in-dogs-with-acute-thoracolumbar-disk-herniation-a-randomized-controlled-clinical-trial
#13
N J Olby, S L Vaden, K Williams, E H Griffith, T Harris, C L Mariani, K R Muñana, P J Early, S R Platt, L Boozer, C Giovanella, R Longshore
BACKGROUND: Dogs with spinal cord injury are at increased risk of developing bacteriuria due to increased residual urine volume. Cranberry extract inhibits binding of E. coli to uroepithelial cells, potentially reducing risk of bacteriuria. HYPOTHESIS: Cranberry extract reduces risk of bacteriuria in dogs after acute TL-IVDH. ANIMALS: Client-owned dogs with acute onset TL-IVDH causing nonambulatory status. METHODS: Randomized, placebo-controlled, blinded, prospective clinical trial...
December 3, 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27914036/prolactin-regulatory-element-binding-protein-is-involved-in-suppression-of-the-adiponectin-gene-in-vivo
#14
X Z Zhang, H Imachi, J Y Lyu, K Fukunaga, S Sato, T Ibata, T Kobayashi, T Yoshimoto, F Kikuchi, T Dong, K Murao
PURPOSE: Prolactin regulatory element-binding protein (PREB), a member of the WD-repeat protein family, has been recognized as a transcriptional factor that regulates prolactin promoter activity in the anterior pituitary of rats. PREB is expressed not only in the pituitary but also in various other tissues, including the adipose tissue. Previous studies have shown that PREB acts as a transcriptional regulator and suppresses the expression of the adiponectin gene in cultured 3T3L1 preadipocytes...
December 2, 2016: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#15
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27914013/inhibitory-effect-of-homocysteine-on-rat-neural-stem-cell-growth-in-vitro-is-associated-with-reduced-protein-levels-and-enzymatic-activities-of-aconitase-and-respiratory-complex-iii
#16
Xu-Mei Zhang, Ya-Qian Zhao, Hai Yan, Huan Liu, Guo-Wei Huang
Increased blood plasma concentration of the sulphur amino acid homocysteine (Hcy) is considered as an independent risk factor of the neurodegenerative diseases. However, the detailed molecular mechanisms by which Hcy leads to neurotoxicity have yet to be clarified. Recent research has suggested that neurotoxicity of Hcy may involve negative regulation of neural stem cell (NSC) proliferation. In the current study, primary NSCs were isolated from neonatal rat brain hippocampus and the inhibition in cell growth was observed after exposure to l50 μM and 500 μM L-Hcy...
December 2, 2016: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/27914008/igf1r-dental-pulp-stem-cells-enhanced-neuroplasticity-in-hypoxia-ischemia-model
#17
Hsiao-Yu Chiu, Chen-Huan Lin, Chung Y Hsu, John Yu, Chia-Hung Hsieh, Woei-Cherng Shyu
Until now, the surface markers of multipotent mesenchymal stem cells (MSCs) had not been fully identified. Here, we found that the IGF1 receptor (IGF1R), regarded as a pluripotent marker of embryonic stem cells (ESCs), was also expressed in human dental pulp derived-mesenchymal stem cells (hDSCs), which displayed a potential for both self-renewal and multipotency. hDSC-secreted IGF1 interacted with IGF1R through an autocrine signaling pathway to maintain this self-renewal and proliferation potential. Stereotaxic implantation of immunosorted IGF1R(+) hDSCs in rats with neonatal hypoxia-ischemia (NHI) promoted neuroplasticity, improving the neurological outcome by increasing expression of the anti-apoptotic protein Bcl-2, which enhanced both neurogenesis and angiogenesis...
December 2, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27913995/adenoma-prevalence-and-distribution-among-us-latino-subgroups-undergoing-screening-colonoscopy
#18
Sumedha V Chablani, Lina Jandorf, Katherine DuHamel, Kristen K Lee, Pathu Sriphanlop, Cristina Villagra, Steven H Itzkowitz
BACKGROUND: Colorectal cancer (CRC) is the second leading malignancy diagnosed among US Latinos. Latinos in the USA represent a heterogeneous amalgam of subgroups varying in genetic background, culture, and socioeconomic status. Little is known about the frequency of CRC precursor lesions found at screening colonoscopy among Latino subgroups. AIM: The aim was to determine the prevalence and distribution of histologically confirmed adenomas found at screening colonoscopy among average-risk, asymptomatic US Latinos according to their subgroup and socio-demographic background...
December 2, 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/27913978/case-series-study-of-invasive-pulmonary-aspergillosis
#19
Ana I Aller-García, Carmen Castro-Méndez, Ana Alastruey-Izquierdo, Elena M Marín-Martínez, Ismail Zakariya-Yousef Breval, Carmen Couto-Caro, Juan C López-Marín, Nicolás Peña-Griñán, Maite Ruiz de Pipaon, Ana M Romero-Mejías, Estrella Martín-Mazuelos
Diagnosis of invasive pulmonary aspergillosis (IPA) is challenging. The objective of the study was to assess the value of microbiological tests to the diagnosis of IPA in the absence of non-specific radiological data. A retrospective study of 23 patients with suspicion of IPA and positivity of some microbiological diagnostic tests was performed. These tests included conventional microbiological culture, detection of Aspergillus galactomannan (GM) antigen and in some patients (1 → 3)-β-D-glucan (BDG) and Aspergillus fumigatus DNA using the LightCycler(®) SeptiFast test...
December 2, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27913916/development-of-international-terminology-and-definitions-for-texture-modified-foods-and-thickened-fluids-used-in-dysphagia-management-the-iddsi-framework
#20
Julie A Y Cichero, Peter Lam, Catriona M Steele, Ben Hanson, Jianshe Chen, Roberto O Dantas, Janice Duivestein, Jun Kayashita, Caroline Lecko, Joseph Murray, Mershen Pillay, Luis Riquelme, Soenke Stanschus
Dysphagia is estimated to affect ~8% of the world's population (~590 million people). Texture-modified foods and thickened drinks are commonly used to reduce the risks of choking and aspiration. The International Dysphagia Diet Standardisation Initiative (IDDSI) was founded with the goal of developing globally standardized terminology and definitions for texture-modified foods and liquids applicable to individuals with dysphagia of all ages, in all care settings, and all cultures. A multi-professional volunteer committee developed a dysphagia diet framework through systematic review and stakeholder consultation...
December 2, 2016: Dysphagia
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