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https://www.readbyqxmd.com/read/29679649/protein-solvent-interfaces-in-human-y145stop-prion-protein-amyloid-fibrils-probed-by-paramagnetic-solid-state-nmr-spectroscopy
#1
Darryl Aucoin, Yongjie Xia, Theint Theint, Philippe S Nadaud, Krystyna Surewicz, Witold K Surewicz, Christopher P Jaroniec
The C-terminally truncated Y145Stop variant of prion protein (PrP23-144), which is associated with heritable PrP cerebral amyloid angiopathy in humans and also capable of triggering a transmissible prion disease in mice, serves as a useful in vitro model for investigating the molecular and structural basis of amyloid strains and cross-seeding specificities. Here, we determine the protein-solvent interfaces in human PrP23-144 amyloid fibrils generated from recombinant 13 C,15 N-enriched protein and incubated in aqueous solution containing paramagnetic Cu(II)-EDTA, by measuring residue-specific 15 N longitudinal paramagnetic relaxation enhancements using two-dimensional magic-angle spinning solid-state NMR spectroscopy...
April 18, 2018: Journal of Structural Biology
https://www.readbyqxmd.com/read/29678307/viability-and-functionality-of-mesenchymal-stromal-cells-loaded-on-collagen-microspheres-and-incorporated-into-plasma-clots-for-orthopaedic-application-effect-of-storage-conditions
#2
Olga Wittig, Dylana Diaz-Solano, José Cardier
BACKGROUND: There is evidence showing that human mesenchymal stromal cells (MSC) seeded on collagen microspheres (CM) and incorporated into platelet rich plasma (PRP) clots induce bone formation. For clinical trials it is very important to establish standardization of storage and shipment conditions to ensure the viability and functionality of cellular products. We investigate the effect of storage temperature and time on the viability and functionality of human MSC seeded on CM and included into PRP clots for using in the further clinical application for bone regeneration...
April 5, 2018: Injury
https://www.readbyqxmd.com/read/29675502/survival-analysis-after-core-decompression-in-association-with-platelet-rich-plasma-mesenchymal-stem-cells-and-synthetic-bone-graft-in-patients-with-osteonecrosis-of-the-femoral-head
#3
Riccardo D'Ambrosi, Elena Biancardi, Giulia Massari, Vincenza Ragone, Renato Mario Facchini
Purpose  The aim of this study was to report the rate of survivorship in patients with osteonecrosis of the femoral head treated with core decompression in association with mesenchymal stem cells (MSCs) implantation, platelet-rich plasma (PRP) injection, and synthetic bone graft. Methods  We evaluated 24 hips in 16 patients, according to Ficat classification, treated by core decompression, injection of PRP and MSCs, and backfilling of the core tract with synthetic bone graft. Survivorship was estimated using Kaplan-Meier curves...
March 2018: Joints
https://www.readbyqxmd.com/read/29675174/a-modular-synthetic-route-to-size-defined-immunogenic-haemophilus-influenzae-b-antigens-is-key-to-the-identification-of-an-octasaccharide-lead-vaccine-candidate
#4
J Y Baek, A Geissner, D C K Rathwell, D Meierhofer, C L Pereira, P H Seeberger
The first glycoconjugate vaccine using isolated glycans was licensed to protect children from Haemophilus influenzae serotype b (Hib) infections. Subsequently, the first semisynthetic glycoconjugate vaccine using a mixture of antigens derived by polymerization targeted the same pathogen. Still, a detailed understanding concerning the correlation between oligosaccharide chain length and the immune response towards the polyribosyl-ribitol-phosphate (PRP) capsular polysaccharide that surrounds Hib remains elusive...
February 7, 2018: Chemical Science
https://www.readbyqxmd.com/read/29668626/effect-of-arthrocentesis-plus-platelet-rich-plasma-and-platelet-rich-plasma-alone-in-the-treatment-of-temporomandibular-joint-osteoarthritis-a-retrospective-matched-cohort-study-a-strobe-compliant-article
#5
Shang-Lun Lin, Chiang-Chin Tsai, Shang-Liang Wu, Shun-Yao Ko, Wei-Fan Chiang, Jung Wu Yang
Although the research on using platelet-rich plasma (PRP) for temporomandibular joint osteoarthritis (TMJ-OA) has advanced, no unified standards exist for determining the joint use of arthrocentesis and the injection dose and frequency of PRP. This study aimed to compare the efficacy of 2 TMJ-OA treatment approaches, arthrocentesis plus platelet-rich plasma (A+PRP) and PRP alone, and attempted to provide another potential treatment option with a single injection of 2 mL of high-concentration and high-purity PRP...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29665123/pilot-study-on-the-correlation-between-dermoscopic-patterns-and-fluorescence-in-situ-hybridization-findings-using-whole-slide-digital-imaging-for-acral-volar-melanocytic-lesions
#6
Dai Ogata, Eiichi Arai, Yoshiya Goto, Tetsuya Tsuchida
Dermoscopic evaluation of acral volar skin is helpful in differentiating malignant melanomas (MM) from benign melanocytic nevi. However, histological diagnosis remains difficult because sufficient evidence of histopathological changes to establish a diagnosis of MM are not easily obtained. The aim of the present study was to evaluate the effective use of fluorescence in situ hybridization (FISH) in the diagnosis of acral volar melanocytic lesions, and to determine whether acral volar melanocytic lesions show characteristic molecular biological features of malignant melanoma via FISH...
April 17, 2018: Journal of Dermatology
https://www.readbyqxmd.com/read/29663706/patient-specific-bioinks-for-3d-bioprinting-of-tissue-engineering-scaffolds
#7
Negar Faramarzi, Iman K Yazdi, Mahboubeh Nabavinia, Andrea Gemma, Adele Fanelli, Andrea Caizzone, Leon M Ptaszek, Indranil Sinha, Ali Khademhosseini, Jeremy N Ruskin, Ali Tamayol
Bioprinting has emerged as a promising tool in tissue engineering and regenerative medicine. Various 3D printing strategies have been developed to enable bioprinting of various biopolymers and hydrogels. However, the incorporation of biological factors has not been well explored. As the importance of personalized medicine is becoming more clear, the need for the development of bioinks containing autologous/patient-specific biological factors for tissue engineering applications becomes more evident. Platelet-rich plasma (PRP) is used as a patient-specific source of autologous growth factors that can be easily incorporated to hydrogels and printed into 3D constructs...
April 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29663626/treatment-of-auricular-keloids-by-triple-combination-therapy-surgical-excision-platelet-rich-plasma-and-cryosurgery
#8
Ehab Zaki Azzam, Salma Samir Omar
BACKGROUND: Surgical excision alone for auricular keloids is frequently followed by recurrence making combining treatment options necessary. OBJECTIVE: We aimed to assess the long-term efficacy of combining surgical excision, cryosurgery, and platelet-rich plasma (PRP) in treatment of auricular keloids. METHODS: This interventional study was carried out on fifty auricular keloids in 50 patients. Patients were subjected to a combination therapy of surgical excision of the keloids, intraoperative cryosurgery, and PRP injections...
April 16, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29661222/platelet-rich-plasma-enhances-the-proliferation-of-human-adipose-stem-cells-through-multiple-signaling-pathways
#9
Fangyuan Lai, Natsuko Kakudo, Naoki Morimoto, Shigeru Taketani, Tomoya Hara, Takeshi Ogawa, Kenji Kusumoto
BACKGROUND: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of several growth factors. Platelet-derived growth factor (PDGF)-BB is a potential mitogen for human adipose-derived stem cells (hASCs). PRP stimulates proliferation of hASCs; however, the signaling pathways activated by PRP remain unclear. METHODS: hASCs were cultured with or without PRP or PDGF-BB, and proliferation was assessed. hASCs were also treated with PRP or PDGF-BB with or without imatinib, which is a PDGF receptor tyrosine kinase inhibitor, or sorafenib, which is a multikinase inhibitor...
April 16, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29657476/platelet-rich-plasma-injections-for-lateral-epicondylitis-of-the-elbow-reduce-the-need-for-surgical-intervention
#10
Graham Hastie, Mazen Soufi, James Wilson, Bibhas Roy
Objective: We aimed to assess the effectiveness of PRP injections in lateral epicondylitis of the elbow as it was felt after PRP introduction the numbers of patients requiring surgery for had reduced. Methods: We conducted a retrospective review of cases from the 1st January 2008 to 31st December 2015. The numbers of patients undergoing surgical release and the numbers of patients requiring PRP injections were recorded each year and the numbers of patients requiring surgery was compared pre and post PRP injection to ascertain if PRP introduction reduced surgical intervention...
March 2018: Journal of Orthopaedics
https://www.readbyqxmd.com/read/29657450/current-advances-in-the-treatment-of-medial-and-lateral-epicondylitis
#11
REVIEW
Sandip P Tarpada, Matthew T Morris, Jayson Lian, Sina Rashidi
Despite advances elucidating the causes of lateral and medial epicondylitis, the standard of care remains conservative management with NSAIDs, physical therapy, bracing, and rest. Scar tissue formation provoked by conservative management creates a tendon lacking the biomechanical properties and mechanical strength of normal tendon. The following review analyzes novel therapies to regenerate tendon and regain function in patients with epicondylitis. These treatments include PRP injection, BMAC, collagen-producing cell injection, and stem cell treatments...
March 2018: Journal of Orthopaedics
https://www.readbyqxmd.com/read/29649372/autogenous-bone-graft-versus-bovine-bone-graft-in-association-with-platelet-rich-plasma-for-the-reconstruction-of-alveolar-clefts-a-pilot-study
#12
Bruno Torres Bezerra, John Nadson Andrade Pinho, Fabrício Eneas Diniz Figueiredo, José Renato Moraes Carvalho Barreto Brandão, Lucas Celestino Guerzet Ayres, Luiz Carlos Ferreira da Silva
OBJECTIVE: To compare the outcomes of 2 different bone graft materials: autogenous bone grafts from mandibular symphysis and bovine bone grafts associated with platelet-rich plasma (PRP). METHODS: Twenty individuals met the inclusion criteria and accepted to participate in the study. Group A patients underwent alveolar bone grafting using autologous bone and group B patients using a bovine bone graft associated with PRP. Cone beam computed tomography scans were taken and reconstructed 3-dimensionally for all patients preoperatively and 1 year postoperatively...
January 1, 2018: Cleft Palate-craniofacial Journal
https://www.readbyqxmd.com/read/29645399/a%C3%AE-induces-prion-protein-amyloid-formation-evidence-for-widespread-amyloidogenic-effect-of-a%C3%AE
#13
Ryo Honda
Transmissible spongiform encephalopathy (TSE) is associated with misfolding of prion protein (PrP) into an amyloid β-rich aggregate. Previous studies have indicated that PrP interacts with Alzheimer disease amyloid-β peptide (Aβ), but it remains elusive how this interaction impacts on the misfolding of PrP. This study presents the first in vitro evidence that Aβ induces PrP-amyloid formation at submicromolar concentrations. Interestingly, systematic mutagenesis of PrP revealed that Aβ requires no specific amino acid sequences in PrP, and induces the misfolding of other unrelated proteins (insulin and lysozyme) into amyloid fibrils in a manner analogous to PrP...
April 12, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29644924/role-of-prion-protein-egfr-multimolecular-complex-during-neuronal-differentiation-of-human-dental-pulp-derived-stem-cells
#14
Stefano Martellucci, Valeria Manganelli, Costantino Santacroce, Francesca Santilli, Luca Piccoli, Maurizio Sorice, Vincenzo Mattei
Cellular prion protein (PrPC ) is expressed in a wide variety of stem cells in which regulates their self-renewal as well as differentiation potential. In this study we investigated the presence of PrPC in human dental pulp-derived stem cells (hDPSCs) and its role in neuronal differentiation process. We show that hDPSCs expresses early PrPC at low concentration and its expression increases after two weeks of treatment with EGF/bFGF. Then, we analyzed the association of PrPC with gangliosides and EGF receptor (EGF-R) during neuronal differentiation process...
April 12, 2018: Prion
https://www.readbyqxmd.com/read/29628381/biological-treatment-for-osteoarthritis-of-the-knee-moving-from-bench-to-bedside-current-practical-concepts
#15
Jorge Chahla, Bert R Mandelbaum
Biological-based therapies for cartilage pathology have gained considerable recognition in the last few decades due to their potential benefits including their minimal invasiveness, capacity for unprecedented healing, and potential for rapid recovery. Consequently, these therapies are likely to have the most noteworthy impact on patients with degenerative joint changes who want to remain active. Currently, the most researched treatments include platelet-rich plasma (PRP), bone marrow aspirate concentrate (BMAC), and cell-based therapies...
April 5, 2018: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29626641/platelet-rich-plasma-versus-hyaluronic-acid-in-patients-with-hip-osteoarthritis-a-meta-analysis-of-randomized-controlled-trials
#16
REVIEW
Ye Ye, Xiang Zhou, Shuiwei Mao, Jun Zhang, Bingmin Lin
BACKGROUND: This study aims to compare the efficacy of intra-articular injection of hyaluronic acid (HA) and platelet-rich plasma (PRP) for treating hip osteoarthritis (OA). METHODS: We performed systematic searches in PubMed, EmBase, ScienceDirect Web of science and the Cochrane Library for relevant literature published in or before February 2018. Only randomized controlled trials (RCTs) were included. The risk of bias assessment was performed using the tool recommended in the Cochrane Handbook for Systematic Reviews of Interventions (version 5...
April 4, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29619095/platelet-rich-plasma-in-reconstruction-of-posterior-meatal-wall-after-canal-wall-down-mastoidectomy
#17
Mohammad El-Sayed Abd Elbary, Wail Fayez Nasr, Samir Sorour Sorour
Introduction  Canal wall down (CWD) mastoidectomy has many drawbacks, including chronic otorrhea not responding to medications, granulations, dizziness on exposure to cold or hot water, and tendency of debris accumulation in the mastoid cavity, demanding periodic cleaning. Many of these problems can be solved by reconstruction of the posterior meatal wall (PMW). Objectives  To assess the results of PMW reconstruction after CWD mastoidectomy for cholesteatoma using titanium mesh and platelet-rich plasma (PRP) mixed with bone pate...
April 2018: International Archives of Otorhinolaryngology
https://www.readbyqxmd.com/read/29618704/pinelliae-rhizoma-praeparatum-involved-in-the-regulation-of-bile-acids-metabolism-in-hepatic-injury
#18
Shun Guo, Song Zhang, Linna Liu, Peng Yang, Xueliang Dang, Huamei Wei, Na Hu, Lei Shi, Yan Zhang
Pinelliae Rhizoma Praeparatum (PRP) as traditional Chinese medicine had been used for hepatic diseases in combinative forms. However, the effect of PRP was not clear when used alone. So to explore the hepatoprotective/hepatotoxin of PRP is necessary. The activities of PRP were investigated in acetaminophen-induced hepatic injury mice. Liver function markers, hepatic oxidative stress markers were evaluated. Bile acids metabolic transports and Nrf2 were detected. As a drug for the treatment of liver diseases, PRP slightly restored the parameters towards normal in model mice only in low dosage, and also had no antioxidant activity and regulate Nrf2...
April 4, 2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29618668/establishment-of-a-madin-darby-bovine-kidney-cell-line-expressing-anchorless-bovine-prion-protein
#19
Hyo-Jin Kim, In-Soon Roh, Hoo-Chang Park, Su Bi Ahn, Tae-Young Suh, Kyung-Je Park, Hae-Eun Kang, Hyun-Joo Sohn
Enzyme-linked immunosorbent assay (ELISA) performed using extensively purified bacterially expressed bovine prion protein (PrP) shows decreased cross-reactivity. We generated a transduced Madin-Darby bovine kidney (MDBK) cell line continuously expressing glycosylphosphatidylinositol (GPI)-anchorless bovine PrP (designated as MDBK ∆GPI protein) by using a lentiviral expression system. The present study also described the method for purifying bovine PrP through sequential culturing without the need for complex purification protocol...
April 4, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29617719/platelet-rich-plasma-adipose-tissue-and-scar-modulation
#20
Lohrasb R Sayadi, Zaidal Obagi, Derek A Banyard, Mary E Ziegler, Jordyne Prussak, Luke Tomlinson, Gregory R D Evans, Alan D Widgerow
In developed countries, over 100 million people per year form scars, of which 30% become hypertrophic. These scars are defined histologically by the presence of persistently contractile myofibroblasts. Recent in vitro research suggests that certain bone morphogenic proteins (BMP) can induce scar myofibroblast dedifferentiation and reprogramming into adipocytes. Since platelets contain BMPs within their granules, it is possible that platelet-rich plasma (PRP) can act as a vehicle to deliver BMP to sites of scarring or potential scarring...
March 30, 2018: Aesthetic Surgery Journal
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