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https://www.readbyqxmd.com/read/28930232/antimicrobial-antioxidant-and-cytotoxic-activities-of-ocimum-forskolei-and-teucrium-yemense-lamiaceae-essential-oils
#1
Nasser A Awadh Ali, Bhuwan K Chhetri, Noura S Dosoky, Khola Shari, Ahmed J A Al-Fahad, Ludger Wessjohann, William N Setzer
Background:Ocimum forskolei and Teucrium yemense (Lamiaceae) are used in traditional medicine in Yemen. Methods: The chemical composition, antimicrobial, antioxidant and cytotoxic activities of the essential oils isolated from the leaves of Ocimum forskolei Benth. (EOOF) and two different populations of Teucrium yemense Deflers., one collected from Dhamar province (EOTY-d), and another collected from Taiz (EOTY-t) were investigated. The antimicrobial activities of the oils were evaluated against several microorganisms with the disc diffusion test or the broth microdilution test...
April 1, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28929579/in-situ-biosensor-for-detection-mirna-in-living-cells-based-on-carbon-nitride-nanosheets-with-catalytic-hairpin-assembly-amplification
#2
Xianjiu Liao, Liqing Li, Jianbin Pan, Tingting Peng, Bin Ge, Qianli Tang
In this study, an ultrasensitive fluorescence turn-on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low-level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector. Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzyme-free fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed...
September 19, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28929019/heterologous-expression-of-plasmodium-vivax-apical-membrane-antigen-1-pvama1-for-binding-peptide-selection
#3
Ching Hoong Chew, Yvonne Ai Lian Lim, Kek Heng Chua
BACKGROUND: Plasmodium is an obligate intracellular parasite. Apical membrane antigen 1 (AMA1) is the most prominent and well characterized malarial surface antigen that is essential for parasite-host cell invasion, i.e., for sporozoite to invade and replicate within hepatocytes in the liver stage and merozoite to penetrate and replicate within erythrocytes in the blood stage. AMA1 has long served as a potent antimalarial drug target and is a pivotal vaccine candidate. A good understanding of the structure and molecular function of this Plasmodium protein, particularly its involvement in host-cell adhesion and invasion, is of great interest and hence it offers an attractive target for the development of novel therapeutics...
2017: PeerJ
https://www.readbyqxmd.com/read/28928512/determination-of-antioxidant-activity-of-hibiscus-sabdariffa-and-croton-caudatus-in-saccharomyces-cerevisiae-model-system
#4
Pattnaik Subhaswaraj, M Sowmya, V Bhavana, Madhu Dyavaiah, Busi Siddhardha
From ancient times, plants and plant derived products are exploited as a prominent source of folkloric medicines with tremendous therapeutic potential for an array of health disorders. In the present study, ethanolic leaf extract of Hibiscus sabdariffa and Croton caudatus were evaluated for free radical scavenging activity in Saccharomyces cerevisiae model system. H. sabdariffa and C. caudatus showed tremendous DPPH free radical scavenging potential with an IC50 value of 184.88 and 305.39 µg/mL respectively at a concentration of 500 µg/mL...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28928380/predictors-of-responses-to-immune-checkpoint-blockade-in-advanced-melanoma
#5
N Jacquelot, M P Roberti, D P Enot, S Rusakiewicz, N Ternès, S Jegou, D M Woods, A L Sodré, M Hansen, Y Meirow, M Sade-Feldman, A Burra, S S Kwek, C Flament, M Messaoudene, C P M Duong, L Chen, B S Kwon, A C Anderson, V K Kuchroo, B Weide, F Aubin, C Borg, S Dalle, O Beatrix, M Ayyoub, B Balme, G Tomasic, A M Di Giacomo, M Maio, D Schadendorf, I Melero, B Dréno, A Khammari, R Dummer, M Levesque, Y Koguchi, L Fong, M Lotem, M Baniyash, H Schmidt, I M Svane, G Kroemer, A Marabelle, S Michiels, A Cavalcanti, M J Smyth, J S Weber, A M Eggermont, L Zitvogel
Immune checkpoint blockers (ICB) have become pivotal therapies in the clinical armamentarium against metastatic melanoma (MMel). Given the frequency of immune related adverse events and increasing use of ICB, predictors of response to CTLA-4 and/or PD-1 blockade represent unmet clinical needs. Using a systems biology-based approach to an assessment of 779 paired blood and tumor markers in 37 stage III MMel patients, we analyzed association between blood immune parameters and the functional immune reactivity of tumor-infiltrating cells after ex vivo exposure to ICB...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28927101/effects-of-p38mapk-mediated-excision-repair-cross-complementation-1-expression-on-prognosis-of-patients-with-non-small-cell-lung-cancer
#6
Dan He, Xiaomei Ma, Zhenhua Wu, Yang Wang, Shuyuan Zhao, Feng Han, Wei Sun
The present study aimed to investigate the effects of excision repair cross-complementation 1 (ERCC1) expression on the prognosis of patients with non-small cell lung cancer (NSCLC). A total of 140 patients with NSCLC who underwent radical resection were included. Immunohistochemical staining was performed on the tissue specimens obtained from patients and correlation analysis was used to determine the association between ERCC1 expression and clinicopathological characteristics. Cell proliferation was assessed using an MTT assay...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927062/anticancer-activity-and-antioxidant-potential-of-aponogeton-undulatus-against-ehrlich-ascites-carcinoma-cells-in-swiss-albino-mice
#7
Mohammad Saifur Rahman, Mohammad Badrul Alam, Yun Hee Choi, Jin Cheol Yoo
The in vivo anticancer and in vitro antioxidant effects of the crude methanol extract of Aponogeton undulatus, in addition to its various organic fractions, were investigated. Various assay methods, including the total antioxidant capacity assay, lipid peroxidation inhibition assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and ferrous reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. The ethyl acetate fraction (EAU) demonstrated the highest antioxidant capacity (175...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927036/urotensin-ii-receptor-as-a-potential-biomarker-for-the-prognosis-of-hepatocellular-carcinoma-patients
#8
Hongtao Wei, Xiaotong Yu, Xiaowei Xue, Hui Liu, Menglong Wang, Yingying Li, Xuejiang Wang, Huiguo Ding
Urotensin II and the associated urotensin II receptor (UTR) are important in the carcinogenesis of hepatocellular carcinoma (HCC). However, the clinical significance of UTR remains to be elucidated. The aim of the present study was to investigate if UTR exhibits the potential to act as a biomarker to predict the prognosis of HCC patients. The effects of UTR on motility and invasion of HCC cells were additionally investigated. UTR expression levels were determined by immunohistochemistry, in 83 HCC patients that previously underwent curative liver resection...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926834/heme-oxygenase-activity-and-heme-binding-in-a-neonatal-mouse-model
#9
Stephanie Kourula, Joyce Ang, Hui Zhao, Flora Kalish, Peter Vandenabeele, Karl G Sylvester, Ronald J Wong, David K Stevenson
BACKGROUND: Severe hemolytic disease of the newborn leads to the release of pro-oxidative free heme (FH). Heme oxygenase (HO) is primarily responsible for detoxifying FH. OBJECTIVE: To investigate the protective effects of HO in a model of heme overload. METHODS: For in vitro studies, NIH3T3 HO-1-luc cells were incubated with 10, 30, or 60 µM FH or methemalbumin (MHA). HO-1 promoter activity was assessed 3, 6, and 24 h after treatment. Cell survival was indexed by viability assays...
September 20, 2017: Neonatology
https://www.readbyqxmd.com/read/28924391/snp-rs3202538-in-3-utr-region-of-erbb3-regulated-by-mir-204-and-mir-211-promote-gastric-cancer-development-in-chinese-population
#10
Yaxiang Shi, Xuan Chen, Biao Xi, Xiaowen Yu, Jun Ouyang, Chunxia Han, Yucheng Qin, Defeng Wu, Hong Shen
BACKGROUND/AIMS: ErbB3 is an oncogene which has proliferation and metastasis promotion effects by several signaling pathways. However, the individual expression difference regulated by miRNA was almost still unknown. We focused on the miRNAs associated SNPs in the 3'-UTR of ErbB3 to investigate the further relationship of the SNPs with miRNAs among Chinese gastric cancer (GC) patients. METHODS: We performed case-control study including 851 GC patients and 799 cancer-free controls...
2017: Cancer Cell International
https://www.readbyqxmd.com/read/28924345/delivery-of-paeonol-by-nanoparticles-enhances-its-in-vitro-and-in-vivo-antitumor-effects
#11
Cong Chen, Feng Jia, Zhibo Hou, Shu Ruan, Qibin Lu
Paeonol (Pae; 2'-hydroxy-4'-methoxyacetophenone) has attracted intense attention as a potential therapeutic agent against various cancers. However, the use of Pae is limited owing to its hydrophobicity. Recently, biodegradable polymeric nanoparticles with amphiphilic copolymers have been used as drug carriers; these have better bioavailability and are promising tumor-targeted drug delivery systems. In the current study, we prepared Pae-loaded nanoparticles (Pae-NPs) with amphiphilic block copolymers using nanoprecipitation...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28922469/one-pot-synthesis-of-gdp-mannose-by-a-multi-enzyme-cascade-for-enzymatic-assembly-of-lipid-linked-oligosaccharides
#12
Thomas F T Rexer, Anna Schildbach, Jan Klapproth, Angelika Schierhorn, Reza Mahour, Markus Pietzsch, Erdmann Rapp, Udo Reichl
Glycosylation of proteins is a key function of the biosynthetic-secretory pathway in the endoplasmic reticulum (ER) and Golgi apparatus. Glycosylated proteins play a crucial role in cell trafficking and signaling, cell-cell adhesion, blood-group antigenicity, and immune response. In addition, the glycosylation of proteins is an important parameter in the optimization of many glycoprotein-based drugs such as monoclonal antibodies. In vitro glycoengineering of proteins requires glycosyltransferases as well as expensive nucleotide sugars...
September 18, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28922377/c-reactive-protein-upregulates-the-whole-blood-expression-of-cd59-an-integrative-analysis
#13
Kaido Lepik, Tarmo Annilo, Viktorija Kukuškina, Kai Kisand, Zoltán Kutalik, Pärt Peterson, Hedi Peterson
Elevated C-reactive protein (CRP) concentrations in the blood are associated with acute and chronic infections and inflammation. Nevertheless, the functional role of increased CRP in multiple bacterial and viral infections as well as in chronic inflammatory diseases remains unclear. Here, we studied the relationship between CRP and gene expression levels in the blood in 491 individuals from the Estonian Biobank cohort, to elucidate the role of CRP in these inflammatory mechanisms. As a result, we identified a set of 1,614 genes associated with changes in CRP levels with a high proportion of interferon-stimulated genes...
September 18, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28922372/reduction-of-organelle-motility-by-removal-of-potassium-and-other-solutes
#14
John W Murray, David Yin, Allan W Wolkoff
There are surprisingly few studies that describe how the composition of cell culture medium may affect the trafficking of organelles. Here we utilize time lapse multi-channel fluorescent imaging to show that short term exposure of Huh-7 cells to medium lacking potassium, sodium, or chloride strongly reduces but does not eliminate the characteristic back and forth and cell-traversing movement of fluorescent EGF (FL-EGF) containing organelles. We focused on potassium because of its relatively low abundance in media and serum and its energy requiring accumulation into cells...
2017: PloS One
https://www.readbyqxmd.com/read/28919741/extracellular-biosynthesis-of-silver-nanoparticles-using-the-cell-free-filtrate-of-nematophagous-fungus-duddingtonia-flagrans
#15
Laryssa Pinheiro Costa Silva, Jairo Pinto Oliveira, Wanderson Juvencio Keijok, André Romero da Silva, Anderson Rocha Aguiar, Marco Cesar Cunegundes Guimarães, Carolina Magri Ferraz, Jackson Victor Araújo, Fernando Luiz Tobias, Fábio Ribeiro Braga
The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO3 solution. They have been characterized by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28919739/docetaxel-titanate-nanotubes-enhance-radiosensitivity-in-an-androgen-independent-prostate-cancer-model
#16
Céline Mirjolet, Julien Boudon, Alexis Loiseau, Sandy Chevrier, Romain Boidot, Alexandra Oudot, Bertrand Collin, Etienne Martin, Pattayil Alias Joy, Nadine Millot, Gilles Créhange
Around 40% of high-risk prostate cancer patients who undergo radiotherapy (RT) will experience biochemical failure. Chemotherapy, such as docetaxel (DTX), can enhance the efficacy of RT. Multidrug resistance mechanisms often limit drug efficacy by decreasing intracellular concentrations of drugs in tumor cells. It is, therefore, of interest to develop nanocarriers of DTX to maintain the drug inside cancer cells and thus improve treatment efficacy. The purpose of this study was to investigate the use of titanate nanotubes (TiONts) to develop a TiONts-DTX nanocarrier and to evaluate its radiosensitizing in vivo efficacy in a prostate cancer model...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28918633/axisymmetric-and-non-axisymmetric-oscillations-of-sessile-compound-droplets-in-open-digital-microfluidic-platform
#17
Shubhi Bansal, Prosenjit Sen
Manipulating droplets of biological fluids in Electrowetting on Dielectric (EWOD) based digital microfluidic platform is a significant challenge due to biofouling and surface contamination. This problem is often addressed by operating in an oil environment. We study an alternate configuration of sessile compound droplets having an aqueous core surrounded by a smaller oil shell. In contrast to conventional EWOD, open digital microfluidic platform enabled by the core-shell configuration will allow electrical, mechanical or optical probes to get unrestricted access to the droplet and thus enabling highly flexible and dynamically reconfigurable lab-on-chip systems...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28916966/evaluation-of-in-vitro-efficacy-of-docetaxel-loaded-calcium-carbonate-aragonite-nanoparticles-dtx-caco3np-on-4t1-mouse-breast-cancer-cell-line
#18
Nahidah Ibrahim Hammadi, Yusuf Abba, Mohd Noor Mohd Hezmee, Intan Shameha Abdul Razak, Aminu Umar Kura, Zuki Abu Bakar Zakaria
Cockle shell-derived calcium carbonate nanoparticles have shown promising potentials as slow drug-releasing compounds in cancer chemotherapy. In this study, we evaluated the in vitro efficacy of docetaxel (DTX)-loaded CaCO3NP on 4T1 cell line. This was achieved by evaluating the following: cytotoxicity using MTT assay, fluorescence imaging, apoptosis with Annexin V assay, cell cycle analysis, scanning (SEM) and transmission electron microscopy (TEM), and scratch assay. Based on the results, DTX-CaCO3NP with a DTX concentration of 0...
September 15, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28916296/folate-targeted-amphiphilic-cyclodextrin-nanoparticles-incorporating-a-fusogenic-peptide-deliver-therapeutic-sirna-and-inhibit-the-invasive-capacity-of-3d-prostate-cancer-tumours
#19
James C Evans, Meenakshi Malhotra, Katrina Sweeney, Raphael Darcy, Colleen C Nelson, Brett G Hollier, Caitriona M O'Driscoll
The main barrier to the development of an effective RNA interference (RNAi) therapy is the lack of a suitable delivery vector. Modified cyclodextrins have emerged in recent years for the delivery of siRNA. In the present study, a folate-targeted amphiphilic cyclodextrin was formulated using DSPE-PEG5000-folate to target prostate cancer cells. The fusogenic peptide GALA was included in the formulation to aid in the endosomal release of siRNA. Targeted nanoparticles were less than 200nm in size with a neutral surface charge...
September 12, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28916035/sensitive-prostate-specific-antigen-quantification-using-dihydrolipoic-acid-surface-functionalized-phosphorescent-quantum-dots
#20
Marta García-Cortés, María Teresa Fernández-Argüelles, José M Costa-Fernández, Alfredo Sanz-Medel
Herein, high-quality Mn-doped ZnS quantum dots (QDs) have been synthesized using a facile approach directly in aqueous media. The surface of the obtained QDs was further modified by cap-exchange of the native cysteine shell with dihydrolipoic acid (DHLA) ligands resulting in nanocrystals with high water-stability having an intense phosphorescent signal. Covalent bioconjugation of the DHLA-coated nanoparticles with an anti-IgG antibody was then carried out. Interestingly the QD immunoprobe (QD-labelled antibodies) maintained an intense phosphorescence emission, without any significant spectral-shift (as compared to the free QDs)...
September 22, 2017: Analytica Chimica Acta
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