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https://www.readbyqxmd.com/read/28640952/label-free-dynamic-mass-redistribution-and-bio-impedance-methods-for-drug-discovery
#1
Manuel Grundmann
Label-free biosensors are increasingly employed in drug discovery. Cell-based biosensors provide valuable insights into the biological consequences of exposing cells and tissues to chemical agents and the underlying molecular mechanisms associated with these effects. Optical biosensors based on the detection of dynamic mass redistribution (DMR) and impedance biosensors using cellular dielectric spectroscopy (CDS) capture changes of the cytoskeleton of living cells in real time. Because signal transduction correlates with changes in cell morphology, DMR and CDS biosensors are exquisitely suited for recording integrated cell responses in an unbiased, yet pathway-specific manner without the use of labels that may interfere with cell function...
June 22, 2017: Current Protocols in Pharmacology
https://www.readbyqxmd.com/read/28636553/the-prognostic-and-diagnostic-value-of-circulating-tumor-cells-in-bladder-cancer-and-upper-tract-urothelial-carcinoma-a-meta-analysis-of-30-published-studies
#2
Zheng Zhang, Wei Fan, Qiaoling Deng, Shihui Tang, Ping Wang, Peipei Xu, June Wang, Mingxia Yu
There are inconsistent conclusions in the association between circulating tumor cells (CTCs) and urothelial cancer (UC). We performed a meta-analysis to assess the prognostic and diagnostic value of CTCs in UC. We search Medline, Embase and Web of science for relevant studies. The study was set up according to the inclusion/exclusion criteria. 30 published studies with a total of 2161 urothelial cancer patients were included. Meta-analysis showed that CTC-positive was significantly associated with tumor stage (≤ II vs III, IV) (OR = 4...
June 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28636544/analysis-of-esr1-and-pik3ca-mutations-in-plasma-cell-free-dna-from-er-positive-breast-cancer-patients
#3
Takashi Takeshita, Yutaka Yamamoto, Mutsuko Yamamoto-Ibusuki, Mai Tomiguchi, Aiko Sueta, Keiichi Murakami, Yoko Omoto, Hirotaka Iwase
BACKGROUND: The measurement of ESR1 and PIK3CA mutations in plasma cell-free DNA (cfDNA) has been studied as a non-invasive method to quickly assess and monitor endocrine therapy (ET) resistant metastatic breast cancer (MBC) patients. METHODS: The subjects of this retrospective study were a total of 185 plasma samples from 86 estrogen receptor-positive BC patients, of which 151 plasma samples were from 69 MBC patients and 34 plasma samples were from 17 primary BC (PBC) patients...
June 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/28636387/novel-kiteplatin-pyrophosphate-derivatives-with-improved-efficacy
#4
Alessandra Curci, Valentina Gandin, Cristina Marzano, James D Hoeschele, Giovanni Natile, Nicola Margiotta
Two new Pt(II) derivatives of kiteplatin ([PtCl2(cis-1,4-DACH)]) with pyrophosphate as carrier ligand, one mononuclear (1) and one dinuclear (2), were synthesized with the aim of potentiating the efficacy of kiteplatin. Complex 1 resulted to be remarkably stable at physiological pH, but it undergoes a fast hydrolysis reaction at acidic pH releasing free pyrophosphate and (aquated) kiteplatin. The dinuclear compound 2 resulted to be less stable than 1 at both neutral and acidic pH forming 1 and (aquated) kiteplatin as first step...
June 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28635256/in-situ-gelation-induced-death-of-cancer-cells-based-on-proteinosomes
#5
Yuting Zhou, Jianmin Song, Lei Wang, Xuting Xue, Xiaoman Liu, Hui Xie, Xin Huang
Hydrogel is quite a nice kind of material, which can be utilized as the platform for cell culture. However, when bulky hydrogel was formed inside cancer cells, results would be changed. In this study, we showed a way of in situ gelation inside the cells which could induce the death of cancer cells. A glutathione (GSH) responsive proteinosomes, with nice biocompatibility, were prepared as carriers of sodium alginate to be endocytosed by cancer cells, where the chelation between sodium alginate and free calcium ions in the culture medium occurred during the diffusion process...
June 21, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28635229/-macrophage-colony-stimulating-factor-enhances-non-small-cell-lung-cancer-invasion-and-metastasis-by-promoting-macrophage-m2-polarization
#6
Y J Li, L Yang, L P Wang, Y Zhang
Objective: To investigate the key cytokine which polarizes M2 macrophages and promotes invasion and metastasis in non-small cell lung cancer (NSCLC). Methods: After co-culture with A549 cells in vitro, the proportion of CD14(+) CD163(+) M2 macrophages in monocytes and macrophage colony stimulating factor (M-CSF) levels in culture supernatant were detected by flow cytometry, ELISA assay and real-time qPCR, respectively. The effects of CD14(+) CD163(+) M2 macrophages on invasion of A549 cells and angiogenesis of HUVEC cells were measured by transwell assay and tubule formation assay, respectively...
June 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28634590/the-immune-epitope-database-how-data-are-entered-and-retrieved
#7
REVIEW
Ward Fleri, Kerrie Vaughan, Nima Salimi, Randi Vita, Bjoern Peters, Alessandro Sette
Easy access to a vast collection of experimental data on immune epitopes can greatly facilitate the development of therapeutics and vaccines. The Immune Epitope Database and Analysis Resource (IEDB) was developed to provide such a resource as a free service to the biomedical research community. The IEDB contains epitope and assay information related to infectious diseases, autoimmune diseases, allergic diseases, and transplant/alloantigens for humans, nonhuman primates, mice, and any other species studied. It contains T cell, B cell, MHC binding, and MHC ligand elution experiments...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28633597/effect-of-t-2-toxin-injected-shrimp-muscle-extracts-on-mouse-macrophage-cells-raw264-7
#8
Zhanrui Huang, Yaling Wang, Mei Qiu, Lijun Sun, Jianmeng Liao, Rundong Wang, Xiaodong Sun, Siyuan Bi, Ravi Gooneratne
Following intramuscular injections of 0.1 mL, 3 mg kg(-1 )BW(-1)(1/10 LD50) T-2 toxin (T-2), the tissue concentration of T-2 in shrimp was quantitatively detected using LC-MS/MS. The biological half-time (t1/2) of T-2 in blood was 40.47 ± 0.24 min. The highest number of intramuscular T-2 shrimp could tolerate when given at blood t1/2 intervals was 4. The shrimps which were injected 5 T-2 died. The T-2 toxin highest accumulation was 0.471 ± 0.012 ng g(-1 )BW(-1). The effect of toxic shrimp muscle subjected to different processing conditions (high pressure, trifluoroacetic acid, acid and alkali digestions, artificial digestive juice [to simulate exposure to gastric and intestinal juices]) on mouse macrophage cells (RAW267...
February 10, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28633033/centrosome-assembly-reconstructing-the-core-cartwheel-structure-in%C3%A2-vitro
#9
Gaëlle Marteil, Marco António Dias Louro, Mónica Bettencourt-Dias
Centrioles are microtubule-based cylinders essential for the formation of centrosomes and cilia. A recent study provides a new cell-free assay that reconstitutes the initial structure formed during centriole assembly - the cartwheel - and proposes a new model for its formation and growth.
June 19, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28629464/mir-629-3p-may-serve-as-a-novel-biomarker-and-potential-therapeutic-target-for-lung-metastases-of-triple-negative-breast-cancer
#10
Jin Wang, Cailu Song, Hailin Tang, Chao Zhang, Jun Tang, Xing Li, Bo Chen, Xiaoming Xie
BACKGROUND: Different breast cancer subtypes show distinct tropisms for sites of metastasis. Notably, the lung is the most common site for the first distant recurrence in triple-negative breast cancer (TNBC). The identification of novel biomarkers for lung metastasis is of great importance to improving the outcome of TNBC. In this study, we sought to identify a microRNA (miRNA)-based biomarker and therapeutic target for lung metastasis of TNBC. METHODS: A total of 669 patients without de novo stage IV TNBC were recruited for this study...
June 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28629450/interaction-with-adipocyte-stromal-cells-induces-breast-cancer-malignancy-via-s100a7-upregulation-in-breast-cancer-microenvironment
#11
Minako Sakurai, Yasuhiro Miki, Kiyoshi Takagi, Takashi Suzuki, Takanori Ishida, Noriaki Ohuchi, Hironobu Sasano
BACKGROUND: Breast adipocytes play important roles in both the development and function of mammary epithelial cells. Therefore, carcinoma-adipose stromal cell (ASC) interactions have been considered pivotal in supporting tumor growth in breast cancer. In addition, it has been demonstrated that the biological features of cancer-associated adipocytes differ from those of normal ASCs. Therefore, we investigated an interaction between ASCs and carcinoma cell lines to identify genes associated with ASC invasion of carcinoma cells...
June 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28629339/droplet-digital-pcr-for-detection-and-quantification-of-circulating-tumor-dna-in-plasma-of-head-and-neck-cancer-patients
#12
Joost H van Ginkel, Manon M H Huibers, Robert J J van Es, Remco de Bree, Stefan M Willems
BACKGROUND: During posttreatment surveillance of head and neck cancer patients, imaging is insufficiently accurate for the early detection of relapsing disease. Free circulating tumor DNA (ctDNA) may serve as a novel biomarker for monitoring tumor burden during posttreatment surveillance of these patients. In this exploratory study, we investigated whether low level ctDNA in plasma of head and neck cancer patients can be detected using Droplet Digital PCR (ddPCR). METHODS: TP53 mutations were determined in surgically resected primary tumor samples from six patients with high stage (II-IV), moderate to poorly differentiated head and neck squamous cell carcinoma (HNSCC)...
June 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28629050/novel-bilayer-wound-dressing-based-on-electrospun-gelatin-keratin-nanofibrous-mats-for-skin-wound-repair
#13
Chun-Hsu Yao, Chia-Yu Lee, Chiung-Hua Huang, Yueh-Sheng Chen, Kuo-Yu Chen
A bilayer membrane (GKU) with a commercial polyurethane wound dressing as an outer layer and electrospun gelatin/keratin nanofibrous mat as an inner layer was fabricated as a novel wound dressing. Scanning electron micrographs showed that gelatin/keratin nanofibers had a uniform morphology and bead-free structure with average fiber diameter of 160.4nm. 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide assay using L929 fibroblast cells indicated that the residues released from the gelatin/keratin composite nanofibrous mat accelerated cell proliferation...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629019/methotrexate-loaded-nitrogen-doped-graphene-quantum-dots-nanocarriers-as-an-efficient-anticancer-drug-delivery-system
#14
Fatemeh Khodadadei, Shahrokh Safarian, Narges Ghanbari
Graphene quantum dots (GQDs) are new efficient nanomaterials used in therapeutic applications. In this study, blue fluorescent nitrogen-doped GQDs (N-GQDs) were synthesized by a hydrothermal method via pyrolisis of citric acid as the carbon source and urea as the nitrogen source. The existence of doped nitrogen in GQDs was confirmed by FTIR characterization. Here, for the first time, the N-GQDs were loaded with the anticancer drug, methotrexate (MTX), to prepare MTX-(N-GQDs) as an efficient drug delivery system...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628229/troxerutin-with-copper-generates-oxidative-stress-in-cancer-cells-its-possible-chemotherapeutic-mechanism-against-hepatocellular-carcinoma
#15
A Subastri, A Suyavaran, Preedia Babu E, Nithyananthan S, R Bharathidasan, C Thirunavukkarasu
Troxerutin (TXER) a rutin derivative is known for its anticancer effect against hepatocellular carcinoma (HCC). As part of large study, recently we have shown TXER interact with genetic material and its anti-mutagenic property. In the present study we have explored its possible mode of action in HCC. Since TXER alone did not show significant anticancer effect on Huh-7 cells, in vitro biochemical assays were performed for determining anticancer efficacy of TXER + metal complex using transition metals such as Cu, Zn & Fe...
June 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28627575/label-free-detection-of-aggregated-platelets-in-blood-by-machine-learning-aided-optofluidic-time-stretch-microscopy
#16
Yiyue Jiang, Cheng Lei, Atsushi Yasumoto, Hirofumi Kobayashi, Yuri Aisaka, Takuro Ito, Baoshan Guo, Nao Nitta, Natsumaro Kutsuna, Yasuyuki Ozeki, Atsuhiro Nakagawa, Yutaka Yatomi, Keisuke Goda
According to WHO, about 10 million new cases of thrombotic disorders are diagnosed worldwide every year. Thrombotic disorders, including atherothrombosis (the leading cause of death in the US and Europe), are induced by occlusion of blood vessels, due to the formation of blood clots in which aggregated platelets play an important role. The presence of aggregated platelets in blood may be related to atherothrombosis (especially acute myocardial infarction) and is, hence, useful as a potential biomarker for the disease...
June 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28627436/folate-targeted-polymersomes-loaded-with-both-paclitaxel-and-doxorubicin-for-the-combination-chemotherapy-of-hepatocellular-carcinoma
#17
Dunwan Zhu, Shengjie Wu, Chunyan Hu, Zhuo Chen, Hai Wang, Fan Fan, Yu Qin, Chun Wang, Hongfan Sun, Xigang Leng, Deling Kong, Linhua Zhang
Combination chemotherapy is a promising method of improving cancer treatment, but the distinct pharmacokinetics of combined drugs and non-specific drug distribution slow down the development in the clinic. In this study, folate (FA) receptor-targeted polymersomes with apparent bilayered lamellar structure were successfully developed to co-encapsulate a hydrophobic-hydrophilic chemotherapeutic drug pair (PTX and DOX) in a single vesicle for enhancing the combination chemotherapeutic effect. Hydrophobic PTX was loaded into the thick hydrophobic lamellar membrane by the self-assembly of triblock copolymer PCL8000-PEG8000-PCL8000, while hydrophilic DOX was encapsulated into the hydrophilic reservoir using a trans-membrane ammonium sulfate gradient method...
June 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28627070/lack-of-immunogenicity-of-hydrolysed-wheat-flour-in-patients-with-coeliac-disease-after-a-short-term-oral-challenge
#18
R Mandile, S Picascia, C Parrella, A Camarca, M Gobbetti, L Greco, R Troncone, C Gianfrani, R Auricchio
BACKGROUND: A gluten-free diet is currently the only reliable therapeutic strategy that is approved for coeliac disease (CD). For many patients, however, compliance remains inadequate. AIM: To investigate the immunogenicity of wheat flour that was pre-treated with selected lactobacilli and fungal proteases (hydrolysed wheat gluten) in coeliac patients. METHODS: The immunogenicity of hydrolysed wheat gluten was evaluated both in vitro in intestinal T cell lines (TCLs) and in vivo in treated CD patients after a short-term gluten challenge...
June 19, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28626365/emergence-of-cd43-expressing-hematopoietic-progenitors-from-human-induced-pluripotent-stem-cells
#19
Katharina U Kessel, Anika Bluemke, Hans R Schöler, Holm Zaehres, Peter Schlenke, Isabel Dorn
BACKGROUND: The ex vivo generation of human hematopoietic stem cells (HSCs) with long-term repopulating capacity and multi-lineage differentiation potential represents the holy grail of hematopoiesis research. In principle, human induced pluripotent stem cells (hiPSCs) provide the tool for both studying molecular mechanisms of hematopoietic development and the ex vivo production of 'true' HSCs for transplantation purposes and lineage-specific cells, e.g. red blood cells, for transfusion purposes...
June 2017: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/28626006/atairp2-e3-ligase-affects-aba-and-high-salinity-responses-by-stimulating-its-atp1-sdirip1-substrate-turnover
#20
Tae Rin Oh, Jong Hum Kim, Seok Keun Cho, Moon Young Ryu, Seong Wook Yang, Woo Taek Kim
AtAIRP2 is a cytosolic RING-type E3 ubiquitin (Ub) ligase that positively regulates an ABA response in Arabidopsis (Arabidopsis thaliana). Yeast two-hybrid screening using AtAIRP2 as bait identified ATP1 (AtAIRP2 Target Protein 1) as a substrate of AtAIRP2. ATP1 was found to be identical to SDIRIP1, which was recently reported to be a negative factor in ABA signaling and a target protein of the RING E3 ligase SDIR1. ATP1 was accordingly renamed ATP1/SDIRIP1. A specific interaction between AtAIRP2 and ATP1/SDIRIP1 and ubiquitination of ATP1/SDIRIP1 by AtAIRP2 were demonstrated in vitro and in planta...
June 16, 2017: Plant Physiology
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