keyword
MENU ▼
Read by QxMD icon Read
search

Signalling

keyword
https://www.readbyqxmd.com/read/29444544/uridine-5-diphospho-glucuronosyltransferase-1a-expression-as-an-independent-prognosticator-in-urothelial-carcinoma-of-the-upper-urinary-tract
#1
Koji Izumi, Satoshi Inoue, Hiroki Ide, Kazutoshi Fujita, Taichi Mizushima, Guiyang Jiang, Seiji Yamaguchi, Hiroaki Fushimi, Norio Nonomura, Hiroshi Miyamoto
OBJECTIVE: To determine the expression status of uridine 5'diphospho-glucuronosyltransferase 1A, a major phase II drug metabolism enzyme, in upper urinary tract urothelial carcinoma, as well as to assess its prognostic significance. METHODS: We immunohistochemically stained for uridine 5'diphospho-glucuronosyltransferase 1A in tissue microarray consisting of 99 upper urinary tract urothelial carcinoma samples and paired non-neoplastic urothelial tissues. We also assessed the effect of uridine 5'diphospho-glucuronosyltransferase 1A knockdown on urothelial cancer cell growth...
February 14, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/29444515/comprehensive-mirna-analysis-of-human-umbilical-cord-derived-mesenchymal-stromal-cells-and-extracellular-vesicles
#2
Xiang-Yu Zou, Yongjiang Yu, Sihao Lin, Liang Zhong, Jie Sun, Guangyuan Zhang, Yingjian Zhu
BACKGROUND/AIMS: Mesenchymal stromal cells (MSCs) participate in the tissue-specific repair of many different organs, especially the kidney. Their effects are primarily mediated by the paracrine release of factors including extracellular vesicles (EVs), which are composed of micro-vesicles and exosomes. The corresponding microRNAs (miRNAs) of EVs are considered important for their biological functions. METHODS: MSCs were cultured from the human umbilical cord, and EVs were isolated from the medium...
February 8, 2018: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29444507/high-uric-acid-inhibits-cardiomyocyte-viability-through-the-erk-p38-pathway-via-oxidative-stress
#3
Zhi Li, Yang Shen, Yingqun Chen, Guokai Zhang, Jidong Cheng, Wei Wang
BACKGROUND/AIMS: Clinical studies have shown that hyperuricaemia is strongly associated with cardiovascular disease. However, the molecular mechanisms of high uric acid (HUA) associated with cardiovascular disease remain poorly understood. In this study, we investigated the effect of HUA on cardiomyocytes. METHODS: We exposed H9c2 cardiomyocytes to HUA, then cell viability was determined by MTT assay, and reactive oxygen species' (ROS) production was detected by a fluorescence assay...
February 8, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29444490/systematic-evaluation-of-matrix-effects-in-supercritical-fluid-chromatography-versus-liquid-chromatography-coupled-to-mass-spectrometry-for-biological-samples
#4
Vincent Desfontaine, Francesca Capetti, Raul Nicoli, Tiia Kuuranne, Jean-Luc Veuthey, Davy Guillarme
Matrix effects (ME) is acknowledged as being one of the major drawbacks of quantitative bioanalytical methods, involving the use of liquid chromatography coupled to mass spectrometry (LC-MS). In the present study, the incidence of ME in SFC-MS/MS and LC-MS/MS in the positive mode electrospray ionization (ESI+) was systematically compared for the analysis of urine and plasma samples using two representative sets of 40 doping agents and 38 pharmaceutical compounds, respectively. Three different SFC stationary phase chemistries were employed, to highlight the importance of the column in terms of selectivity...
February 7, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29444487/paracrine-fibroblast-growth-factor-initiates-oncogenic-synergy-with-epithelial-fgfr-src-transformation-in-prostate-tumor-progression
#5
Qianjin Li, Lishann Ingram, Sungjin Kim, Zanna Beharry, Jonathan A Cooper, Houjian Cai
Cross talk of stromal-epithelial cells plays an essential role in both normal development and tumor initiation and progression. Fibroblast growth factor (FGF)-FGF receptor (FGFR)-Src kinase axis is one of the major signal transduction pathways to mediate this cross talk. Numerous genomic studies have demonstrated that expression levels of FGFR/Src are deregulated in a variety of cancers including prostate cancer; however, the role that paracrine FGF (from stromal cells) plays in dysregulated expression of epithelial FGFRs/Src and tumor progression in vivo is not well evaluated...
February 11, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29444480/does-crash-risk-increase-when-emergency-vehicles-are-driving-with-lights-and-sirens
#6
Celestin Missikpode, Corinne Peek-Asa, Tracy Young, Cara Hamann
INTRODUCTION: Emergency vehicles, such as police, ambulances, and fire vehicles, need to arrive at the scene of emergencies as quickly as possible, and thus they often travel in emergency mode - using their lights and sirens and often bypassing traffic signals. We examined whether travelling in emergency mode increased crash risk among police, ambulance and fire vehicles. METHODS: We conducted a quasi-induced exposure analysis using data from the Iowa Crash Database for the period of 2005 through 2013...
February 10, 2018: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/29444478/detection-of-drug-safety-signals-from-clinical-trials-data-role-of-susars
#7
Christelle Perez, Pascale Olivier, Barbara Lortal, Sophie Duranton, Jean-Louis Montastruc, Anne-Laurène Colin, Emilie Toulza, Madlyne Becker, Laura Hamy, Sabrina Crepin, Caroline Roussillon, Anne Gimbert, Nadine Petitpain, Francesco Salvo
One of the main goals of safety management in clinical trials is to detect suspected unexpected serious adverse reactions (SUSARs). The unexpectedness concerns the nature, frequency or severity of an adverse reaction. Drug safety signals could thus be retrieved, and a study was performed to investigate whether SUSARs allow signal detection in pharmacovigilance. Data from six academic safety units were collected from 2005 to 2016. Characteristics of SUSARs were analysed and signals were identified i) by evaluating the presence of other causes, ii) by assessing the summary of product characteristics (SPC), iii) by searching for specific safety information in Pubmed and health agencies, and iv) by investigating the narrative of each case...
February 11, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29444476/retraction-notice-to-in%C3%A2-vitro-modeling-of-blood-brain-barrier-with-human-ipsc-derived-endothelial-cells-pericytes-neurons-and-astrocytes-via-notch-signaling
#8
Kohei Yamamizu, Mio Iwasaki, Hitomi Takakubo, Takumi Sakamoto, Takeshi Ikuno, Mami Miyoshi, Takayuki Kondo, Yoichi Nakao, Masato Nakagawa, Haruhisa Inoue, Jun K Yamashita
No abstract text is available yet for this article.
February 13, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29444462/photodynamic-therapy-as-a-new-approach-to-trichomonas-vaginalis-inactivation
#9
Thaisa Helena Silva Fonseca, Marina Alacoque, Fabrício Marcus Silva Oliveira, Betânia Maria Soares, Henrique Vitor Leite, Marcelo Vidigal Caliari, Maria Aparecida Gomes, Haendel Busatti
The emergence of nitroimidazole resistant isolates has been an aggravating factor in the treatment of trichomoniasis, the most common non-viral sexually transmitted disease in the world. This particularity highlights the importance of new technologies that are safe, effective, and have minor side effects or resistance. Hence, we evaluated the effectiveness of photodynamic therapy on the inactivation of Trichomonas vaginalis in vitro. We used methylene blue as a photosensitizing substance, and a light-emitting diode (LED) for irradiation of metronidazole sensitive and resistant strains...
February 11, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29444453/efferocytosis-in-atherosclerotic-lesions-malfunctioning-regulatory-pathways-and-control-mechanisms
#10
REVIEW
Amir Tajbakhsh, Mehdi Rezaee, Petri T Kovanen, Amirhossein Sahebkar
Atherosclerosis is a dynamic and progressive inflammatory process in the intimal layer of large and medium-sized arteries, and it is the major contributor to the atherosclerotic cardiovascular disease (ACVD), the leading cause of death worldwide. In an atherosclerotic plaque, phagocytosis of apoptotic cells occurs through an intricate process designated efferocytosis. Defective efferocytosis has emerged as a causal factor in the etiopathogenesis of atherosclerosis and its progression into overt ACVD. Both specialized phagocytes (macrophages and dendritic cells) and non-specialized cells with phagocytic capabilities (smooth muscle and endothelial cells) are involved in the efferocytotic process...
February 11, 2018: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29444450/high-throughput-screening-identifies-small-molecules-that-bind-to-the-ras-sos-ras-complex-and-perturb-ras-signaling
#11
Michael C Burns, Jennifer E Howes, Qi Sun, Andrew J Little, DeMarco V Camper, Jason R Abbott, Jason Phan, Taekyu Lee, Alex G Waterson, Olivia W Rossanese, Stephen W Fesik
K-RAS is mutated in approximately 30% of human cancers, resulting in increased RAS signaling and tumor growth. Thus, RAS is a highly validated therapeutic target, especially in tumors of the pancreas, lung and colon. Although directly targeting RAS has proven to be challenging, it may be possible to target other proteins involved in RAS signaling, such as the guanine nucleotide exchange factor Son of Sevenless (SOS). We have previously reported on the discovery of small molecules that bind to SOS1, activate SOS-mediated nucleotide exchange on RAS, and paradoxically inhibit ERK phosphorylation (Burns et al...
February 11, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29444439/differential-effector-engagement-by-oncogenic-kras
#12
Tina L Yuan, Arnaud Amzallag, Rachel Bagni, Ming Yi, Shervin Afghani, William Burgan, Nicole Fer, Leslie A Strathern, Katie Powell, Brian Smith, Andrew M Waters, David Drubin, Ty Thomson, Rosy Liao, Patricia Greninger, Giovanna T Stein, Ellen Murchie, Eliane Cortez, Regina K Egan, Lauren Procter, Matthew Bess, Kwong Tai Cheng, Chih-Shia Lee, Liam Changwoo Lee, Christof Fellmann, Robert Stephens, Ji Luo, Scott W Lowe, Cyril H Benes, Frank McCormick
KRAS can bind numerous effector proteins, which activate different downstream signaling events. The best known are RAF, phosphatidylinositide (PI)-3' kinase, and RalGDS families, but many additional direct and indirect effectors have been reported. We have assessed how these effectors contribute to several major phenotypes in a quantitative way, using an arrayed combinatorial siRNA screen in which we knocked down 41 KRAS effectors nodes in 92 cell lines. We show that every cell line has a unique combination of effector dependencies, but in spite of this heterogeneity, we were able to identify two major subtypes of KRAS mutant cancers of the lung, pancreas, and large intestine, which reflect different KRAS effector engagement and opportunities for therapeutic intervention...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444433/microrna-223-suppresses-the-canonical-nf-%C3%AE%C2%BAb-pathway-in-basal-keratinocytes-to-dampen-neutrophilic-inflammation
#13
Wenqing Zhou, Arpita S Pal, Alan Yi-Hui Hsu, Theodore Gurol, Xiaoguang Zhu, Sara E Wirbisky-Hershberger, Jennifer L Freeman, Andrea L Kasinski, Qing Deng
MicroRNA-223 is known as a myeloid-enriched anti-inflammatory microRNA that is dysregulated in numerous inflammatory conditions. Here, we report that neutrophilic inflammation (wound response) is augmented in miR-223-deficient zebrafish, due primarily to elevated activation of the canonical nuclear factor κB (NF-κB) pathway. NF-κB over-activation is restricted to the basal layer of the surface epithelium, although miR-223 is detected throughout the epithelium and in phagocytes. Not only phagocytes but also epithelial cells are involved in miR-223-mediated regulation of neutrophils' wound response and NF-κB activation...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444426/inferior-olive-hcn1-channels-coordinate-synaptic-integration-and-complex-spike-timing
#14
Derek L F Garden, Marlies Oostland, Marta Jelitai, Arianna Rinaldi, Ian Duguid, Matthew F Nolan
Cerebellar climbing-fiber-mediated complex spikes originate from neurons in the inferior olive (IO), are critical for motor coordination, and are central to theories of cerebellar learning. Hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels expressed by IO neurons have been considered as pacemaker currents important for oscillatory and resonant dynamics. Here, we demonstrate that in vitro, network actions of HCN1 channels enable bidirectional glutamatergic synaptic responses, while local actions of HCN1 channels determine the timing and waveform of synaptically driven action potentials...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444423/nuclear-organization-in-the-spinal-cord-depends-on-motor-neuron-lamination-orchestrated-by-catenin-and-afadin-function
#15
Carola Dewitz, Sofia Pimpinella, Patrick Hackel, Altuna Akalin, Thomas M Jessell, Niccolò Zampieri
Motor neurons in the spinal cord are found grouped in nuclear structures termed pools, whose position is precisely orchestrated during development. Despite the emerging role of pool organization in the assembly of spinal circuits, little is known about the morphogenetic programs underlying the patterning of motor neuron subtypes. We applied three-dimensional analysis of motor neuron position to reveal the roles and contributions of cell adhesive function by inactivating N-cadherin, catenin, and afadin signaling...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444421/chloroplast-signaling-gates-thermotolerance-in-arabidopsis
#16
Patrick J Dickinson, Manoj Kumar, Claudia Martinho, Seong Jeon Yoo, Hui Lan, George Artavanis, Varodom Charoensawan, Mark Aurel Schöttler, Ralph Bock, Katja E Jaeger, Philip A Wigge
Temperature is a key environmental variable influencing plant growth and survival. Protection against high temperature stress in eukaryotes is coordinated by heat shock factors (HSFs), transcription factors that activate the expression of protective chaperones such as HEAT SHOCK PROTEIN 70 (HSP70); however, the pathway by which temperature is sensed and integrated with other environmental signals into adaptive responses is not well understood. Plants are exposed to considerable diurnal variation in temperature, and we have found that there is diurnal variation in thermotolerance in Arabidopsis thaliana, with maximal thermotolerance coinciding with higher HSP70 expression during the day...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444420/perineurial-barrier-glia-physically-respond-to-alcohol-in-an-akap200-dependent-manner-to-promote-tolerance
#17
Sarah J Parkhurst, Pratik Adhikari, Jovana S Navarrete, Arièle Legendre, Miguel Manansala, Fred W Wolf
Ethanol is the most common drug of abuse. It exerts its behavioral effects by acting on widespread neural circuits; however, its impact on glial cells is less understood. We show that Drosophila perineurial glia are critical for ethanol tolerance, a simple form of behavioral plasticity. The perineurial glia form the continuous outer cellular layer of the blood-brain barrier and are the interface between the brain and the circulation. Ethanol tolerance development requires the A kinase anchoring protein Akap200 specifically in perineurial glia...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444418/circuitry-rewiring-directly-couples-competence-to-predation-in-the-gut-dweller-streptococcus-salivarius
#18
Johann Mignolet, Laetitia Fontaine, Andrea Sass, Catherine Nannan, Jacques Mahillon, Tom Coenye, Pascal Hols
Small distortions in transcriptional networks might lead to drastic phenotypical changes, especially in cellular developmental programs such as competence for natural transformation. Here, we report a pervasive circuitry rewiring for competence and predation interplay in commensal streptococci. Canonically, in streptococci paradigms such as Streptococcus pneumoniae and Streptococcus mutans, the pheromone-based two-component system BlpRH is a central node that orchestrates the production of antimicrobial compounds (bacteriocins) and incorporates signal from the competence activation cascade...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29444410/enhanced-ion-transmission-efficiency-up-to-m-z-24-000-for-maldi-protein-imaging-mass-spectrometry
#19
Boone M Prentice, Daniel Ryan, Raf Van de Plas, Richard M Caprioli, Jeffrey M Spraggins
The molecular identification of species of interest is an important part of an imaging mass spectrometry (IMS) experiment. The high resolution accurate mass capabilities of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) have recently been shown to facilitate the identification of proteins in matrix-assisted laser desorption/ionization (MALDI) IMS. However, these experiments are typically limited to proteins giving rise to ions of relatively low m/z due to difficulties transmitting and measuring large molecular weight ions of low charge states...
February 14, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29444341/high-fat-diet-induced-obesity-promotes-expansion-of-bone-marrow-adipose-tissue-and-impairs-skeletal-stem-cell-functions-in-mice
#20
Michaela Tencerova, Florence Figeac, Nicholas Ditzel, Hanna Taipaleenmäki, Tina Kamilla Nielsen, Moustapha Kassem
Obesity represents a risk factor for development of insulin resistance and Type 2 Diabetes. In addition, it has been associated with increased adipocyte formation in the bone marrow (BM) along with increased risk for bone fragility fractures. However, little is known on the cellular mechanisms that link obesity, BM adiposity and bone fragility. Thus, in an obesity intervention study in C57BL/6J mice fed with high fat diet (HFD) for 12 weeks, we investigated the molecular and cellular phenotype of bone marrow adipose tissue (BMAT), BM progenitor cells and BM microenvironment in comparison to peripheral adipose tissue (AT)...
February 14, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
keyword
keyword
38099
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"