keyword
MENU ▼
Read by QxMD icon Read
search

Signalling

keyword
https://www.readbyqxmd.com/read/28550769/mk-8776-a-novel-chk1-inhibitor-exhibits-an-improved-radiosensitizing-effect-compared-to-ucn-01-by-exacerbating-radiation-induced-aberrant-mitosis
#1
Motofumi Suzuki, Tohru Yamamori, Tomoki Bo, Yuri Sakai, Osamu Inanami
Checkpoint kinase 1 (Chk1) is an evolutionarily conserved serine/threonine kinase that plays an important role in G2/M checkpoint signaling. Here, we evaluate the radiosensitizing effects of a novel selective Chk1 inhibitor MK-8776, comparing its efficacy with a first-generation Chk1 inhibitor UCN-01, and attempt to elucidate the mechanism of radiosensitization. In a clonogenic survival assay, MK-8776 demonstrated a more pronounced radiosensitizing effect than UCN-01, with lower cytotoxicity. Importantly, radiosensitization by MK-8776 can be achieved at doses as low as 2...
May 24, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28550740/multi-resolution-classification-of-exhaled-aerosol-images-to-detect-obstructive-lung-diseases-in-small-airways
#2
Jinxiang Xi, Weizhong Zhao, Jiayao Eddie Yuan, Biwei Cao, Linlin Zhao
Exhaled aerosol patterns have been used to detect obstructive respiratory diseases in the upper airways. Signals from small airway diseases are weak and may not manifest themselves in the exhaled aerosol patterns. Therefore, it will be more challenging to detect abnormalities in small airways. The objective of this study is to develop a simulation-based classification model that can accurately classify small airway diseases. The model performance was evaluated in five obstructed models that are located in lung bifurcations G7-9...
May 20, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28550735/clarithromycin-inhibits-tnf-%C3%AE-induced-muc5ac-mucin-gene-expression-via-the-mkp-1-p38mapk-dependent-pathway
#3
Said Ahmad Shah, Hajime Ishinaga, Kazuhiko Takeuchi
Clarithromycin is a 14-membered macrolide antibiotic. Low-dose, long-term macrolide therapy is effective in patients with chronic airway diseases, such as diffuse panbronchitis, chronic bronchitis, and chronic sinusitis. However, the mechanism underlying this clinical efficacy remains unclear. The dual specificity phosphatase MKP-1 (MAPK phosphatase-1), also called DUSP (dual specificity phosphatase-1), was initially identified as an in vitro ERK-specific phosphatase, but depending on the cell type, it can also dephosphorylate other members of the MAPK family, such as p38 and JNK, and thus suppress downstream signaling of these kinases...
May 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28550734/pterostilbene-alleviates-polymicrobial-sepsis-induced-liver-injury-possible-role-of-sirt1-signaling
#4
Xiaojing Liu, Xueliang Yang, Lingna Han, Feng Ye, Min Liu, Wanhu Fan, Kai Zhang, Ying Kong, Jian Zhang, Lei Shi, Yunru Chen, Xi Zhang, Shumei Lin
Liver injury occurs frequently during sepsis. Pterostilbene (Pte), a natural dimethylated analog of resveratrol from blueberries, exerts anti-inflammatory and anti-apoptotic effects in various diseases. However, the role of Pte in sepsis-induced liver injury and its underlying mechanisms remain unknown. The current study aimed to evaluate the protective effects of Pte on sepsis-induced liver injury and its potential mechanisms. Sepsis was induced using cecal ligation and puncture (CLP) in C57BL/6 mice. Mice were administered Pte (5, 10, 15mg/kg, i...
May 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28550733/immune-regulation-mechanism-of-astragaloside-iv-on-raw264-7-cells-through-activating-the-nf-%C3%AE%C2%BAb-mapk-signaling-pathway
#5
Yu Li, Tingting Meng, Ning Hao, Huanqing Tao, Suping Zou, Manman Li, Pengfei Ming, Hongyan Ding, Jihong Dong, Shibin Feng, Jinchun Li, Xichun Wang, Jinjie Wu
The present study was designed to investigate the effects of Astragaloside IV (ASIV) on the immune functions of RAW264.7 cells. Compared with control group, the concentrations of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α) and nitric oxide (NO) were higher in the 100μg/mL ASIV-treatment group. The interleukin 6 (IL-6) concentration was significantly higher in the 50 and 100μg/mL ASIV-treatment groups. The relative mRNA expression levels of IL-1β, TNF-α and inducible nitric oxide synthase (iNOS) were significantly higher in the 50 and 100μg/mL ASIV-treatment groups...
May 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28550731/xanthotoxin-suppresses-lps-induced-expression-of-inos-cox-2-tnf-%C3%AE-and-il-6-via-ap-1-nf-%C3%AE%C2%BAb-and-jak-stat-inactivation-in-raw-264-7-macrophages
#6
Seung-Bin Lee, Woo Seok Lee, Ji-Sun Shin, Dae Sik Jang, Kyung Tae Lee
Although xanthotoxin has been reported to possess skin-protective and anti-oxidative properties, its anti-inflammatory capacity has not been studied to date. Therefore, we investigated this role as well as the molecular mechanisms of xanthotoxin in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Xanthotoxin inhibited production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF-α), and interleukin-6 (IL-6) by the LPS-induced macrophages in a concentration-dependent manner. It also suppressed the LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression at the protein levels and iNOS, COX-2, TNF-α, and IL-6 at the mRNA levels...
May 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28550686/microrna-186-suppresses-cell-proliferation-and-metastasis-through-targeting-sentrin-specific-protease-1-in-renal-cell-carcinoma
#7
Dan Jiao, Man Wu, Lei Ji, Feng Liu, Yingying Liu
Recent evidence suggests that dysregulation of microRNAs are associated with the development of multiple malignancies.miR-186 has been reported as a critical cancer regulator in several types of cancers. However, its functional significance and molecular mechanism underlying renal cell carcinoma (RCC) remains unknown. In this study, our results showed that miR-186 expression was dramatically downregulated in RCC tissues and cell lines compared to that in adjacent normal tissues and cell lines. Overexpression of miR-186 significantly inhibited cell growth, colony formation, cell invasion, caused cell cycle arrest at G0/G1 phase, and induced cell apoptosis as detected by MTT, colony formation, Transwell assay, and flow cytometry assays in RCC cells...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550685/long-noncoding-rna-camta1-promotes-proliferation-and-mobility-of-human-breast-cancer-cell-line-mda-mb-231-via-targeting-mir-20b
#8
Pengwei Lu, Yuanting Gu, Lin Li, Fang Wang, Xue Yang, Yunqing Yang
Breast cancer is a serious threat to women's physical and psychological health. Long non-coding RNA CAMTA1 (LncCAMTA1)was believed to be related with tumor progression, but its role in breast cancer is not clear. Human breast cancer cell line MDA-MB-231 was used to investigate effect of LncCAMTA1 on cell viability, migration/invasion, and apoptosis. Expression of LncCAMTA1, miR-20b and VEGF in MDA-MB-231were measured after corresponding transfections. Binding effects between LncCAMTA1 and miR-20b, miR-20b and VEGF 3'UTR were measured...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550679/downregulation-of-src-kinase-signaling-inhibitor-1-srcin1-expression-by-microrna-32-promotes-proliferation-and-epithelial-mesenchymal-transition-in-human-liver-cancer-cells
#9
Ren Chen, Jin-Yao Liao, Jing Huang, Wen-Li Chen, Xiao-Jun Ma, Xiao-Dan Luo
MicroRNAs play an important role in regulating gene expression by binding to the 3' UTR of target mRNAs. In this studywe have made an attempt to assess the molecular mechanisms by which miR-32 suppresses the expression of SRCIN1, thereby leading to promotion of proliferation and epithelial-mesenchymal transition of human liver cancer cells. Human liver cancer cell lines HepG2 were transfected with miR-32 mimics and its control. The HepG2 cell lines were the assessed for miR-32 expression. The transfected cell lines were then studied for SRCIN1 expression by luciferase assay, effect of transfection on cell proliferation and finally epithelial-mesenchymal transition...
May 22, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550678/knockdown-of-long-noncoding-rna-lncrna-metastasis-associated-lung-adenocarcinoma-transcript-1-malat1-inhibits-proliferation-migration-and-invasion-and-promoted-apoptosis-by-targeting-mir-124-in-retinoblastoma
#10
Shujun Liu, Guigang Yan, Junfu Zhang, Lianzhi Yu
Evidence suggests that the long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)is upregulated in cancer tissues and its elevated expression is associated with hyper-proliferation. However, the underlying mechanisms regarding the role of MALAT1 in retinoblastoma (RB) remains unclear. This study aimed to explore the functional role of MALAT1 in RB by targeting miR-124. Results showed that the expression of - MALAT1 was significantly higher in Y79 cell line than ARPE-19 cell line (P<0...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550530/rtms-ameliorates-ptsd-symptoms-in-rats-by-enhancing-glutamate-transmission-and-synaptic-plasticity-in-the-acc-via-the-pten-akt-signalling-pathway
#11
Gaohua Liu, Dayun Feng, Jian Wang, Haifeng Zhang, Zhengwu Peng, Min Cai, Jing Yang, Ruiguo Zhang, Huaning Wang, Shengxi Wu, Qingrong Tan
Repetitive transcranial magnetic stimulation (rTMS) is a novel physiological therapy that has been adopted to clinically treat psychiatric disorders. Our previous study indicated the potential therapeutic effect of rTMS on posttraumatic stress disorder (PTSD). However, the exact molecular mechanism is elusive. Currently, using the single prolonged stress (SPS) rat model for PTSD, we investigated the glutamatergic transmission and neural plasticity changes in the anterior cingulate cortex (ACC) after SPS induction and explored the protective effects and mechanism of rTMS treatment...
May 26, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28550517/early-prediction-of-autoimmune-type-1-diabetes
#12
REVIEW
Simon E Regnell, Åke Lernmark
Underlying type 1 diabetes is a genetic aetiology dominated by the influence of specific HLA haplotypes involving primarily the class II DR-DQ region. In genetically predisposed children with the DR4-DQ8 haplotype, exogenous factors, yet to be identified, are thought to trigger an autoimmune reaction against insulin, signalled by insulin autoantibodies as the first autoantibody to appear. In children with the DR3-DQ2 haplotype, the triggering reaction is primarily against GAD signalled by GAD autoantibodies (GADA) as the first-appearing autoantibody...
May 26, 2017: Diabetologia
https://www.readbyqxmd.com/read/28550455/-effects-of-the-novel-relatively-short-acting-kappa-opioid-receptor-antagonist-ly2444296-in-behaviors-observed-after-chronic-extended-access-cocaine-self-administration-in-rats
#13
Marta Valenza, Eduardo R Butelman, Mary Jeanne Kreek
RATIONALE: The recruitment of the stress circuitry contributes to a shift from positive to negative reinforcement mechanisms sustaining long-term cocaine addiction. The kappa opioid receptor (KOPr) signaling is upregulated by stress and chronic cocaine exposure. While KOPr agonists induce anhedonia and dysphoria, KOPr antagonists display antidepressant and anxiolytic properties. Most of the knowledge on KOPr antagonism is based on drugs with unusual pharmacokinetic and pharmacodynamic properties, complicating interpretation of results...
May 27, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28550445/%C3%AE-carotene-at-physiologically-attainable-concentration-induces-apoptosis-and-down-regulates-cell-survival-and-antioxidant-markers-in-human-breast-cancer-mcf-7-cells
#14
G Sowmya Shree, K Yogendra Prasad, H S Arpitha, U R Deepika, K Nawneet Kumar, Priya Mondal, P Ganesan
Although β-carotene is known for its anti-carcinogenic and antioxidant properties, a few recent epidemiological and experimental evidence show that at higher concentration it acts as pro-oxidant and induces cancer. Since the global burden of breast cancer exceeds all other types of cancer, and its incidence rates is also in increasing trend, the present study attempted to evaluate the anti-cancer molecular mechanism of β-carotene (at 1 µM concentration) isolated from Spinacia oleracea in human breast cancer (MCF-7) cells...
May 26, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28550425/cpg-oligonucleotide-mediated-co-stimulation-of-mouse-invariant-natural-killer-t-cells-negatively-regulates-their-activation-status
#15
Raveendra R Kulkarni, Alexander Ian Villanueva, Leah R Read, Jennifer T Brisbin, Siddhartha Kumar Bhaumik, Jonathan LaMarre, Kaja Murali-Krishna, Shayan Sharif
Invariant natural killer T (iNKT) cells play important roles in antimicrobial defense and immune-regulation. We have previously shown that iNKT cells express certain toll-like receptors (TLR), and that TLR co-stimulation of iNKT cells in the presence of suboptimal concentrations of T cell receptor (TCR) agonists enhances cellular activation. In the present study, we investigated the regulatory effects of CpG oligonucleotides in mouse primary hepatic and splenic iNKT cells and in DN32.D3 iNKT cells. We show that CpG treatment of iNKT cells in the presence of higher concentrations of TCR agonists (α-GalCer or anti-CD3 mAb) results in the up-regulation of TLR9 in iNKT cells with a concurrent reduction in their cellular activation, as assessed by their production of IL-2, IL-4 and IFN-γ compared with controls...
May 27, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28550410/different-exogenous-sugars-affect-the-hormone-signal-pathway-and-sugar-metabolism-in-red-globe-vitis-vinifera-l-plantlets-grown-in-vitro-as-shown-by-transcriptomic-analysis
#16
Juan Mao, Wenfang Li, Baoqin Mi, Mohammed Mujitaba Dawuda, Alejandro Calderón-Urrea, Zonghuan Ma, Yongmei Zhang, Baihong Chen
Exogenously applied 2% fructose is the most appropriate carbon source that enhances photosynthesis and growth of grape plantlets compared with the same concentrations of sucrose and glucose. The role of the sugars was regulated by the expression of key candidate genes related to hormones, key metabolic enzymes, and sugar metabolism of grape plantlets ( Vitis vinifera L.) grown in vitro. The addition of sugars including sucrose, glucose, and fructose is known to be very helpful for the development of grape (V...
May 26, 2017: Planta
https://www.readbyqxmd.com/read/28550404/development-of-an-imaging-system-for-in-vivo-real-time-monitoring-of-neuronal-activity-in-deep-brain-of-free-moving-rats
#17
Norio Iijima, Shinji Miyamoto, Keisuke Matsumoto, Ken Takumi, Yoichi Ueta, Hitoshi Ozawa
We have newly developed a system that allows monitoring of the intensity of fluorescent signals from deep brains of rats transgenically modified to express enhanced green fluorescent protein (eGFP) via an optical fiber. One terminal of the optical fiber was connected to a blue semiconductor laser oscillator/green fluorescence detector. The other terminal was inserted into the vicinity of the eGFP-expressing neurons. Since the optical fiber was vulnerable to twisting stresses caused by animal movement, we also developed a cage in which the floor automatically turns, in response to the turning of the rat's head...
May 26, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28550396/genistein-protects-against-ox-ldl-induced-inflammation-through-microrna-155-socs1-mediated-repression-of-nf-%C3%A4-b-signaling-pathway-in-huvecs
#18
Huaping Zhang, Zhenxiang Zhao, Xuefen Pang, Jian Yang, Haixia Yu, Yinhong Zhang, Hui Zhou, Jiahui Zhao
Genistein plays an important role in the prevention of atherosclerosis. However, the underlying mechanisms have not been fully investigated. In this study, human umbilical vein endothelial cells (HUVECs) were pretreated with genistein (10, 100, and 1000 nM) for 6 h and then exposed to ox-LDL (50 mg/L) for another 24 h. Results showed that ox-LDL induced the expressions of E-selectin, P-selectin, monocyte chemotactic protein-1, interleukin-8, vascular adhesion molecule-1, and intercellular adhesion molecule-1, which were counteracted by genistein...
May 27, 2017: Inflammation
https://www.readbyqxmd.com/read/28550395/a-critical-role-for-il-21-receptor-signaling-in-the-coxsackievirus-b3-induced-myocarditis
#19
Fan Yang, Xiao-Mou Wei, Wen-Wu Liang, Wen-Hong Mo, Bao-Ping Tan, Hong Wang
To determine whether IL-21 receptor signaling plays a significant role in promoting Tfh cell-mediated cardiac injury in viral myocarditis (VMC), we compared IL-21R-deficient mice for some parameters of VMC. Balb/c and IL-21R(-/-) mice were infected with CVB3. Frequencies of splenic Tfh cells were determined by flow cytometric analysis, and productions of anti-adenine nucleotide translocator (ANT) autoantibodies were detected by enzyme-linked immunosorbent assay. To determine the effects of IL-21R signal on the proliferation of B cells, lymphocytes from spleens of the IL-21R(-/-) and Balb/c mice infected by CVB3 were tagged with carboxyfluorescein succinimidyl ester (CFSE) and then were stimulated with lipopolysaccharides plus IL-21 or anti-IL-21 neutralizing antibody for 3 days...
May 26, 2017: Inflammation
https://www.readbyqxmd.com/read/28550373/black-carp-vasa-identifies-embryonic-and-gonadal-germ-cells
#20
Ting Xue, Miao Yu, Qihua Pan, Yizhou Wang, Jian Fang, Lingyu Li, Yu Deng, Kai Chen, Qian Wang, Tiansheng Chen
Identification of molecular markers is an essential step in the study of germ cells. Vasa is an RNA helicase and a well-known germ cell marker that plays a crucial role in germ cell development. Here, we identified the Vasa homolog termed Mpvasa as the first germ cell marker in black carp (Mylopharyngodon piceus). First, a 2819-bp full-length Mpvasa complementary DNA (cDNA) was cloned by PCR using degenerated primers of conserved sequences and gene-specific primers. The Mpvasa cDNA sequence encodes a 637-amino acid protein that contains eight conserved characteristic motifs of the DEAD box protein family, and shares high identity to grass carp (81%) and zebrafish (74%) vasa homologs...
May 26, 2017: Development Genes and Evolution
keyword
keyword
38099
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"