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Xiaobo Cai, Jun Li, Xiaodong Yuan, Jingbo Xiao, Steven Dooley, Xinjian Wan, Honglei Weng, Lungen Lu
BACKGROUND: CD133 is a marker of stem cells as well cancer stem cells. This study investigated the association between CD133 expression in cancer cells and the clinical outcome of non-mucin producing intrahepatic cholangiocarcinoma (ICC). METHODS: Fifty-seven non-mucin producing ICC patients were enrolled in this study. Immunohistochemistry (IHC) and immunofluorescence staining for CD133 as well as other cancer-associated proteins, including cytokeratin 19, TGF-β1, p-Smad2 and epithelial-mesenchymal transition (EMT) markers S100A4, E-Cadherin and Vimentin were analyzed...
March 6, 2018: Journal of Translational Medicine
Noemi Eiro, Lucía González, Anxo Martínez-Ordoñez, Belen Fernandez-Garcia, Luis O González, Sandra Cid, Francisco Dominguez, Román Perez-Fernandez, Francisco J Vizoso
PURPOSE: It has been reported that stromal cell features may affect the clinical outcome of breast cancer patients. Cancer associated fibroblasts (CAFs) represent one of the most abundant cell types within the breast cancer stroma. Here, we aimed to explore the influence of CAFs on breast cancer gene expression, as well as on invasion and angiogenesis. METHODS: qRT-PCR was used to evaluate the expression of several cancer progression related genes (S100A4, TGFβ, FGF2, FGF7, PDGFA, PDGFB, VEGFA, IL-6, IL-8, uPA, MMP2, MMP9, MMP11 and TIMP1) in the human breast cancer-derived cell lines MCF-7 and MDA-MB-231, before and after co-culture with CAFs...
March 1, 2018: Cellular Oncology (Dordrecht)
Muhammad Arsalan Raffat, Naila Irum Hadi, Mervyn Hosein, Sana Mirza, Sana Ikram, Zohaib Akram
BACKGROUND: Controversy exists in the literature regarding the differential expression of S100 protein members and their functional correlations in oral squamous cell carcinoma (OSCC). The aim of the present study was to systematically review the expression of S100 protein family members among OSCC and healthy controls and to evaluate whether S100 protein members serve as diagnostic marker in OSCC. METHODS: Indexed databases were searched up to and including October 2017...
February 14, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Peter Arner, Paul Petrus, David Esteve, Anne Boulomié, Erik Näslund, Anders Thorell, Hui Gao, Ingrid Dahlman, Mikael Rydén
BACKGROUND: Adipokines are peptides secreted from white adipose tissue (WAT), which have been linked to WAT dysfunction and metabolic complications of obesity. We set out to identify novel adipokines in subcutaneous WAT (sWAT) linked to insulin resistance (IR). METHODS: Gene expression was determined by microarray and qPCR in obese and non-obese subjects with varying degree of IR. WAT-secreted and circulating protein levels were measured by ELISA. RESULTS: In sWAT of 80 obese women discordant for IR, 44 genes encoding potential adipose-secreted proteins were differentially expressed...
January 30, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Shasha Hou, Tian Tian, Dianwen Qi, Kaiji Sun, Qi Yuan, Ziling Wang, Zhihai Qin, Zhenlong Wu, Zhinan Chen, Jinhua Zhang
Autophagy has emerged as a critical pathway in tumor development. S100A4 plays important roles in tumor metastasis, but its role in regulating autophagy has not been well characterized. In this study, we found that S100A4 was significantly upregulated in lung adenocarcinoma tissues. Clinical investigation demonstrated that high expression level of S100A4 was associated with tumor size and advanced tumor grades of lung adenocarcinoma patients. Moreover, our results revealed that extracellular S100A4 or overexpression of S100A4 inhibited starvation-induced autophagy and promoted cell proliferation in lung cancer cells in vitro; whereas small interfering RNA (siRNA)-mediated suppression of S100A4 increased autophagy and reduced cell viability in both A549 and LLC cells...
February 15, 2018: Cell Death & Disease
Jun Li, Peng-Wen Wu, Yuan Zhou, Bo Dai, Peng-Fei Zhang, Yu-Hen Zhang, Yang Liu, Xiao-Lei Shi
The receptor for advanced glycation end products (Rage) is involved in the development of various tumors and acts as an oncogenic protein. Rage is overexpressed in tumors including hepatocellular carcinoma (HCC). However, the molecular mechanism of Rage in HCC progression and sorafenib resistance remains unclear. In this study, enhanced Rage expression is highly associated proliferation and contributes to sorafenib resistance. Rage deficiency contributed to autophagy induction through activating AMPK/mTOR signaling pathway, which is important for sorafenib response...
February 14, 2018: Cell Death & Disease
Y Lv, Z Niu, X Guo, F Yuan, Y Liu
No abstract text is available yet for this article.
February 9, 2018: British Journal of Biomedical Science
Yuan Liu, Changping Men, Yingmin Xu, Kai Zhao, Lei Luo, Dahai Dong, Qinchao Yu
BACKGROUND AND OBJECTIVE: Clusterin promotes cell proliferation, motility and invasiveness in human renal cell carcinoma (RCC) cells but the underlying molecular mechanisms of this action are largely unknown. The aim of this study was to investigate the effects of clusterin on cancer cell growth, invasion and S100A4 expression and to determine the effects of clusterin on in vitro cell proliferation and migration and in vivo tumour growth in RCC cells. METHODS: We have established stable transfectants of highly invasive Caki-1 human RCC cells with expression of clusterin shRNA targeting clusterin (Caki-1/clusterin shRNA)...
January 19, 2018: Cancer Biomarkers: Section A of Disease Markers
Zhigui Zuo, Peili Zhang, Feiyan Lin, Wenjing Shang, Ruichun Bi, Fengying Lu, Jianbo Wu, Lei Jiang
We previously reported a novel positive feedback loop between thioredoxin-1 (Trx-1) and S100P, which promotes the invasion and metastasis of colorectal cancer (CRC). However, the underlying molecular mechanisms remain poorly understood. In this study, we examined the roles of Trx-1 and S100P in CRC epithelial-to-mesenchymal transition (EMT) and their underlying mechanisms. We observed that knockdown of Trx-1 or S100P in SW620 cells inhibited EMT, whereas overexpression of Trx-1 or S100P in SW480 cells promoted EMT...
January 31, 2018: Journal of Cellular and Molecular Medicine
William Chang, Michelle Lajko, Amani A Fawzi
PURPOSE: To characterize the relationship between endothelin-1 and fibrosis in epiretinal membranes in proliferative diabetic retinopathy and explore the role of endothelial-mesenchymal transition in these membranes. METHODS: Membranes were obtained from eyes undergoing pars plana vitrectomy for complicated proliferative diabetic retinopathy or idiopathic epiretinal membrane. Through standard immunohistochemical techniques, we labeled membranes to explore the distribution of endothelin-1 and endothelin receptor B, comparing proliferative diabetic retinopathy and idiopathic epiretinal membranes...
2018: PloS One
Bikesh Nirala, David Baskin, Kyuson Yun
No abstract text is available yet for this article.
November 2017: Oncoscience
Yue Bian, Junfu Guo, Linlin Qiao, Xiuju Sun
GDF15 is a downstream gene of S100A4. miR-3189 is embedded in the intron of GDF15-and coexpressed with it. miR-3189-3p functions to inhibit the proliferation and migration of glioblastoma cells. We speculated that S100A4 might regulate miR-3189-3p to affect its function in gastric cancer cells. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) showed that miR-3189-3p expression was significantly downregulated in MGC803 cells after S100A4 knockdown. Overexpression of miR-3189-3p significantly inhibited the proliferation and migration of the cells...
January 13, 2018: International Journal of Molecular Sciences
Christiane Gebhard, Ingrid Miller, Karin Hummel, Martina Neschi Née Ondrovics, Sarah Schlosser, Ingrid Walter
Osteosarcoma is an aggressive bone tumor with high metastasis rate in the lungs and affects both humans and dogs in a similar way. Three-dimensional tumor cell cultures mimic the in vivo situation of micro-tumors and metastases and are therefore better experimental in vitro models than the often applied two-dimensional monolayer cultures. The aim of the present study was to perform comparative proteomics of standard monolayer cultures of canine osteosarcoma cells (D17) and three-dimensional spheroid cultures, to better characterize the 3D model before starting with experiments like migration assays...
January 11, 2018: Journal of Proteomics
Catharina Melzer, Juliane von der Ohe, Ralf Hass
BACKGROUND: Fusion of breast cancer cells with tumor-associated populations of the microenvironment including mesenchymal stroma/stem-like cells (MSC) represents a rare event in cell communication whereby the metastatic capacity of those hybrid cells remains unclear. METHODS: Functional changes were investigated in vitro and in vivo following spontaneous fusion and hybrid cell formation between primary human MSC and human MDA-MB-231 breast cancer cells. Thus, lentiviral eGFP-labeled MSC and breast cancer cells labeled with mcherry resulted in dual-fluorescing hybrid cells after co-culture...
January 5, 2018: Cell Communication and Signaling: CCS
Jingjing Jiao, Álvaro González, Heather L Stevenson, Mihai Gagea, Hikaru Sugimoto, Raghu Kalluri, Laura Beretta
There is a pressing need for the development of novel approaches to treat and prevent hepatocellular carcinoma (HCC). The S100 calcium-binding protein S100A4 is associated with poor prognosis and metastasis in several human cancers. In addition, a role for S100A4 in modulating cancer-initiating cells stemness properties was recently proposed in head and neck and gastric cancers. Whether S100A4+ stromal cells contribute to tumor onset remains, however, an unanswered question. To address that question, we generated a new mouse model allowing for the depletion of S100A4+ cells in a mouse model of HCC with stemness properties, by crossing mice with hepatic deletion of phosphatase and tensin homolog (PTEN) with mice expressing viral thymidine kinase under the control of S100A4 promoter...
January 5, 2018: Experimental & Molecular Medicine
Yi Xiao, Quan Zhao, Bo Du, Hua-Yan Chen, Dao-Zheng Zhou
Abnormal expression and dysfunction of microRNAs are correlated with osteosarcoma (OS). This study demonstrated that the miR-187 level in OS tissues and cell lines was decreased. The proliferation and metastatic abilities of U-2OS cells were inhibited by miR-187 overexpression and promoted by miR-187 knockdown. Moreover, miR-187 also inhibited growth and metastasis of OS cells in vivo. Furthermore, we revealed that miR-187 could interact with S100A4 3'-UTR and inhibit S100A4 expression in OS cells. In summary, miR-187 inhibits growth and metastasis of OS cells by downregulating S100A4, which might be a potential biomarker and therapeutic target of OS...
January 5, 2018: Cancer Investigation
Natalya G Dulyaninova, Penelope D Ruiz, Matthew J Gamble, Jonathan M Backer, Anne R Bresnick
S100A4, a member of the S100 family of Ca2+-binding proteins, is a key regulator of cell migration and invasion. Our previous studies showed that bone marrow-derived macrophages from S100A4-/- mice exhibit defects in directional motility and chemotaxis in vitro, as well as reduced recruitment to sites of inflammation in vivo (Li et al., 2010). We now show that the loss of S100A4 produces two mechanistically distinct phenotypes with regard to macrophage invasion: a defect in matrix degradation, due to a disruption of podosome rosettes caused by myosin-IIA overassembly, and a myosin-independent increase in microtubule acetylation, which increases podosome rosette stability and is sufficient to inhibit macrophage invasion...
December 27, 2017: Molecular Biology of the Cell
E A Dukhanina, T I Lukyanova, A S Dukhanin, S G Georgieva
S100A4 is a Ca2+-binding protein that performs an important role in metastasis. It is also known for its antitumor functions. S100A4 is expressed by a specialized subset of CD4+CD25+ lymphocytes and is present on those cell's membranes along with peptidoglycan recognition proteins (PGRPs). There, by interacting with major heat shock protein Hsp70, S100A4 plays an important cytotoxic role. The resulting stably formed complex of PGRPs, S100A4 and Hsp70 is required for the identification and binding between a lymphocyte and a target cell...
December 17, 2017: Cell Cycle
Dengke Yang, Guang Du, An Xu, Xuetao Xi, Dong Li
MicroRNAs play key roles during various crucial cell processes, such as proliferation, migration, and invasion. In addition, microRNAs have been shown to possess oncogenic and tumor suppressive functions in human cancers. Increasing evidence has clarified that miR-149-3p, a novel cancer-related microRNA, plays an important role in suppression of proliferation, migration, and invasion; however, the effect and mechanisms underlying the miR-149-3p effect in bladder cancer (BCa) remain unclear. In the current study we found that the increased expression of miR-149-3p significantly suppressed cell proliferation, migration, and invasion ability in BCa...
2017: American Journal of Cancer Research
Rasheed Zakaria, Angela Platt-Higgins, Nitika Rathi, Mark Radon, Sumit Das, Kumar Das, Maneesh Bhojak, Andrew Brodbelt, Emmanuel Chavredakis, Michael D Jenkinson, Philip S Rudland
Brain metastases are common and are usually detected by MRI. Diffusion tensor imaging (DTI) is a derivative MRI technique that can detect disruption of white matter tracts in the brain. We have matched preoperative DTI with image-guided sampling of the brain-tumor interface in 26 patients during resection of a brain metastasis and assessed mean diffusivity and fractional anisotropy (FA). The tissue samples were analyzed for vascularity, inflammatory cell infiltration, growth pattern, and tumor expression of proteins associated with growth or local invasion such as Ki67, S100A4, and MMP2, 9, and 13...
February 1, 2018: Cancer Research
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