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S100a4

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https://www.readbyqxmd.com/read/28100681/lfa-1-activation-in-nk-cells-and-their-subsets-influence-of-receptors-maturation-and-cytokine-stimulation
#1
Doris Urlaub, Kristine Höfer, Martha-Lena Müller, Carsten Watzl
The integrin LFA-1 is essential for efficient activation and for cytotoxicity of NK cells because it initiates the assembly of the immunological synapse and mediates firm adhesion to the target. LFA-1 is also needed to polarize the cytotoxic machinery of the NK cell toward the target cell. The binding affinity and avidity of integrins can be regulated via inside-out signals from other receptors. In this article, we investigate the signals necessary to activate LFA-1 in human NK cells. Our data show that LFA-1 has a low ligand-binding activity in resting human NK cells, but it can be stimulated by triggering activating receptors, such as 2B4 or CD16, or by coactivation of different receptor combinations...
January 18, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28074628/s100a4-metastasin-positive-mesenchymal-canine-mammary-tumour-spheroids-reduce-tenascin-c-synthesis-under-dmso-exposure-in-vitro
#2
S Kau, I Miller, A Tichy, C Gabriel
In breast cancer research S100A4-positive tumour-associated stromal cells are assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4/TNC positive stromal canine mammary tumour (CMT) cells. Cells grown as scaffold-free spheroids were investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well-known side effects on cells including TNC expression...
January 11, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28074300/solving-the-crystal-structure-of-human-calcium-free-s100z-the-siege-and-conquer-of-one-of-the-last-s100-family-strongholds
#3
V Calderone, M Fragai, G Gallo, C Luchinat
The X-ray structure of human apo-S100Z has been solved and compared with that of the zebrafish calcium-bound S100Z, which is the closest in sequence. Human apo-S100A12, which shows only 43% sequence identity to human S100Z, has been used as template model to solve the crystallographic phase problem. Although a significant buried surface area between the two physiological dimers is present in the asymmetric unit of human apo-S100Z, the protein does not form the superhelical arrangement in the crystal as observed for the zebrafish calcium-bound S100Z and human calcium-bound S100A4...
January 10, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28062404/endothelial-to-mesenchymal-transition-contributes-to-endothelial-dysfunction-and-dermal-fibrosis-in-systemic-sclerosis
#4
Mirko Manetti, Eloisa Romano, Irene Rosa, Serena Guiducci, Silvia Bellando-Randone, Amato De Paulis, Lidia Ibba-Manneschi, Marco Matucci-Cerinic
OBJECTIVE: Systemic sclerosis (SSc) features multiorgan fibrosis orchestrated predominantly by activated myofibroblasts. Endothelial-to-mesenchymal transition (EndoMT) is a transdifferentiation by which endothelial cells (ECs) lose their specific morphology/markers and acquire myofibroblast-like features. Here, we determined the possible contribution of EndoMT to the pathogenesis of dermal fibrosis in SSc and two mouse models. METHODS: Skin sections were immunostained for endothelial CD31 or vascular endothelial (VE)-cadherin in combination with α-smooth muscle actin (α-SMA) myofibroblast marker...
January 6, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28058579/prognostic-significance-of-s100a4-expression-and-subcellular-localization-in-early-stage-breast-cancer
#5
Eivind Valen Egeland, Kjetil Boye, Daehoon Park, Marit Synnestvedt, Torill Sauer, Bjørn Naume, Elin Borgen, Gunhild M Mælandsmo
PURPOSE: Prognostic factors are useful in order to identify early-stage breast cancer patients who might benefit from adjuvant treatment. The metastasis-promoting protein S100A4 has previously been associated with poor prognosis in breast cancer patients. The protein is expressed in diverse subcellular compartments, including the cytoplasm, extracellular space, and nucleus. Nuclear expression is an independent predictor of poor outcome in several cancer types, but the significance of subcellular expression has not yet been assessed in breast cancer...
January 5, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28007662/nitric-oxide-mediated-resistance-to-photodynamic-therapy-in-a-human-breast-tumor-xenograft-model-improved-outcome-with-nos2-inhibitors
#6
Jonathan M Fahey, Albert W Girotti
Many malignant tumors employ iNOS-derived NO to resist eradication by chemotherapeutic agents or ionizing radiation. In this study, we determined whether human breast carcinoma MDA-MB-231 cells in vitro and in vivo as tumor xenografts would exploit endogenous iNOS/NO to resist the cytotoxic effects of 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT). Broad band visible irradiation of ALA-treated cells resulted in a marked after-light upregulation of iNOS protein which persisted for at least 24 h...
January 30, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27998393/extracellular-s100a4-negatively-regulates-osteoblast-function-by-activating-the-nf-%C3%AE%C2%BAb-pathway
#7
Haemin Kim, Yong Deok Lee, Min Kyung Kim, Jun-Oh Kwon, Min-Kyoung Song, Zang Hee Lee, Hong-Hee Kim
Patients with inflammatory bone disease or cancer have an increased risk of fractures and delayed healing of bones. S100A4 protein is a member of the calcium-binding S100 protein family, which is abundantly expressed in inflammatory diseases and cancers. In this study, we investigated the effects of extracellular S100A4 on osteoblasts, cells responsible for bone formation. Treatment of mouse primary calvarial osteoblasts with recombinant S100A4 resulted in reductions in matrix mineralization. The expression of osteoblast marker genes including osteocalcin and osterix was also suppressed...
December 21, 2016: BMB Reports
https://www.readbyqxmd.com/read/27991924/cooperation-among-heterogeneous-prostate-cancer-cells-in-the-bone-metastatic-niche
#8
K Shahriari, F Shen, A Worrede-Mahdi, Q Liu, Y Gong, F U Garcia, A Fatatis
The growth of disseminated tumor cells into metastatic lesions depends on the establishment of a favorable microenvironment in the stroma of the target organs. Here we show that mice treated with anakinra, an antagonist of the interleukin (IL)-1β receptor (IL-1R), or harboring a targeted deletion of IL-1R are significantly less prone to develop bone tumors when inoculated in the arterial circulation with human prostate cancer (PCa) cells expressing IL-1β. Interestingly, human mesenchymal stem cells exposed in vitro to medium conditioned by IL-1β-expressing cancer cells responded by upregulating S100A4, a marker of cancer-associated fibroblasts (CAFs), and this effect was blocked by anakinra...
December 19, 2016: Oncogene
https://www.readbyqxmd.com/read/27974362/sharpin-regulates-collagen-architecture-and-ductal-outgrowth-in-the-developing-mouse-mammary-gland
#9
Emilia Peuhu, Riina Kaukonen, Martina Lerche, Markku Saari, Camilo Guzmán, Pia Rantakari, Nicola De Franceschi, Anni Wärri, Maria Georgiadou, Guillaume Jacquemet, Elina Mattila, Reetta Virtakoivu, Yuming Liu, Youmna Attieh, Kathleen A Silva, Timo Betz, John P Sundberg, Marko Salmi, Marie-Ange Deugnier, Kevin W Eliceiri, Johanna Ivaska
SHARPIN is a widely expressed multifunctional protein implicated in cancer, inflammation, linear ubiquitination and integrin activity inhibition; however, its contribution to epithelial homeostasis remains poorly understood. Here, we examined the role of SHARPIN in mammary gland development, a process strongly regulated by epithelial-stromal interactions. Mice lacking SHARPIN expression in all cells (Sharpin(cpdm)), and mice with a stromal (S100a4-Cre) deletion of Sharpin, have reduced mammary ductal outgrowth during puberty...
January 17, 2017: EMBO Journal
https://www.readbyqxmd.com/read/27943262/contribution-of-upregulated-dipeptidyl-peptidase-9-dpp9-in-promoting-tumoregenicity-metastasis-and-the-prediction-of-poor-prognosis-in-non-small-cell-lung-cancer-nsclc
#10
Zhiyuan Tang, Jun Li, Qin Shen, Jian Feng, Hua Liu, Wei Wang, Liqin Xu, Guanglin Shi, Xumei Ye, Min Ge, Xiaoyu Zhou, Songshi Ni
Dipeptidyl peptidase 9 (DPP9) is encoded by DPP9, which belongs to the DPP4 gene family. Proteins encoded by these genes have unique peptidase and extra-enzymatic functions that have been linked to various diseases including cancers. Here, we describe the expression pattern and biological function of DPP9 in non-small-cell lung cancer (NSCLC). The repression of DPP9 expression by small interfering RNA inhibited cell proliferation, migration, and invasion. Moreover, we explored the role of DPP9 in regulating epithelial-mesenchymal transition (EMT)...
December 10, 2016: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27938856/endothelial-mesenchymal-transition-in-human-atrial-fibrillation
#11
Takeshi Kato, Akiko Sekiguchi, Koichi Sagara, Hiroaki Tanabe, Masayuki Takamura, Shuichi Kaneko, Tadanori Aizawa, Long-Tai Fu, Takeshi Yamashita
BACKGROUND: Atrial fibrosis is a hallmark of atrial structural remodeling leading to the persistence of atrial fibrillation. Although fibroblasts play a major role in atrial fibrosis, their source in the adult atrium is unclear. We tested the hypothesis that endothelial cells contribute to fibroblast accumulation through an endothelial-mesenchymal transition in the atrium of patients with atrial fibrillation. METHODS AND RESULTS: The study group consisted of patients with atrial fibrillation and valvular disease or atrial septal defect who underwent left atrial appendectomy during cardiac surgery (n=38)...
December 6, 2016: Journal of Cardiology
https://www.readbyqxmd.com/read/27927689/s100a4-elevation-empowers-expression-of-metastasis-effector-molecules-in-human-breast-cancer
#12
Thamir M M Ismail, Daimark Bennett, Angela M Platt-Higgins, Morteta Al-Medhtiy, Roger Barraclough, Philip S Rudland
Many human glandular cancers metastasize along nerve tracts, but the mechanisms involved are generally poorly understood. The calcium-binding protein S100A4 is expressed at elevated levels in human cancers where it has been linked to increased invasion and metastasis. Here we report genetic studies in a Drosophila model to define S100A4 effector functions that mediate metastatic dissemination of mutant Ras-induced tumors in the developing nervous system. In flies overexpressing mutant RasVal12 and S100A4, there was a significant increase in activation of the stress kinase JNK and production of the matrix metalloproteinase MMP1...
November 10, 2016: Cancer Research
https://www.readbyqxmd.com/read/27922683/molecular-inhibition-mechanisms-of-cell-migration-and-invasion-by-coix-polysaccharides-in-a549-nsclc-cells-via-targeting-s100a4
#13
Cheng Luo, Xin Wang, Can An, Chin-Fa Hwang, Wenhua Miao, Lu Yang, Maonian Xu, Aiping Bai, Shanggui Deng
S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non‑small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively...
January 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27882546/human-gingival-fibroblasts-induced-and-differentiated-into-vascular-endothelial-like-cells
#14
Xuqian Liu, Jie Wang, Fusheng Dong, Hexiang Li, Yali Hou
A novel method for repair of vascular disease, mechanical damage, and tissue rebuilding is urgently required. Vascular endothelial cells (VECs) play an essential role in vascular rebuilding and vasotransplantation. In the present study, human gingival fibroblasts (HGFs) were cultured and induced into endothelial-like cells in vitro in order to confirm that HGFs with stem cell properties possessed the potential for differentiation into endothelial-like cells. The epithelium was extracted from normal human gingiva consisting of epithelium and connective tissue, which was isolated from patients...
December 2016: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/27881889/expression-of-caf-related-proteins-is-associated-with-histologic-grade-of-breast-phyllodes-tumor
#15
Hye Min Kim, Yu Kyung Lee, Ja Seung Koo
Purpose. The purpose of this study was to investigate the expression of cancer-associated fibroblast- (CAF-) related proteins and the implications in breast phyllodes tumor (PT). Methods. Tissue microarrays of 194 PT cases (151 benign PT, 27 borderline PT, and 16 malignant PT) were constructed. We performed immunohistochemical staining for CAF-related proteins (podoplanin, prolyl 4-hydroxylase, FAPα, S100A4, PDGFR α/β, and NG2) and analyzed the results according to clinicopathologic parameters. Results. Expression of PDGFRα and PDGFRβ in the stromal component increased with increasing histologic grade of PT (p = 0...
2016: Disease Markers
https://www.readbyqxmd.com/read/27881141/analysis-of-epithelial-mesenchymal-transition-markers-in-the-histogenesis-of-hepatic-progenitor-cell-in-hbv-related-liver-diseases
#16
Wei Xu, Nong-Rong Wang, Hua-Feng Wang, Qiong Feng, Jun Deng, Zhi-Qiang Gong, Jian Sun, Xiao-Liang Lou, Xue-Feng Yu, Lv Zhou, Jin-Ping Hu, Xiao-Feng Huang, Xiao-Qing Qi, Yan-Juan Deng, Rui Gong, Yan Guo, Meng-Meng Wang, Jia-Cheng Xiao, Huan Deng
BACKGROUND: The origin and heterogeneity of hepatic progenitor cells (HPCs) remain unclear. This study aimed to investigate the involvement of epithelial-mesenchymal transition (EMT) in the histogenesis of HPCs. METHODS: Surgical liver specimens from patients with HBV-related hepatitis and cirrhosis were investigated with double immunofluorescence labeling to detect antigens associated with HPCs and EMT. Ductular reactions were subjected to quantitative reverse transcription PCR following isolation by laser capture microdissection...
November 24, 2016: Diagnostic Pathology
https://www.readbyqxmd.com/read/27863213/tubular-cytoplasmic-expression-of-zinc-finger-protein-snai1-in-renal-transplant-biopsies-a-sign-of-diseased-epithelial-phenotype
#17
Marco Prunotto, Lyubov Chaykovska, Massimo Bongiovanni, Milo Frattini, Thomas Cagarelli, Franziska Weibel, Maurizio Bruschi, Antonio Garcia de Herreros, Solange Moll
The aim of the present study was to analyze in vivo the role of zinc finger protein SNAI1 (SNAI1) on renal fibrosis. Unilateral ureteral obstruction injury was induced in Snai1 knockout mice. Snai1 gene deletion was, however, only partial and could therefore not be correlated to reduced fibrosis. Expression of SNAI1 protein and epithelial-mesenchymal transformation markers was then assessed in human chronic allograft nephropathy biopsy specimens. Significant up-regulation of SNAI1 protein was detected within cytoplasm of proximal tubules localized, for some of them, near foci of fibrosis and tubular atrophy...
January 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/27862618/scleraxis-lineage-cells-contribute-to-ectopic-bone-formation-in-muscle-and-tendon
#18
Shailesh Agarwal, Shawn J Loder, David Cholok, Joshua Peterson, John Li, Christopher Breuler, R Cameron Brownley, Hsiao Hsin Sung, Mike Chung, Nobuhiro Kamiya, Shuli Li, Bin Zhao, Vesa Kaartinen, Thomas A Davis, Ammar T Qureshi, Ernestina Schipani, Yuji Mishina, Benjamin Levi
The pathologic development of heterotopic ossification (HO) is well described in patients with extensive trauma or with hyperactivating mutations of the bone morphogenetic protein (BMP) receptor ACVR1. However, identification of progenitor cells contributing to this process remains elusive. Here we show that connective tissue cells contribute to a substantial amount of HO anlagen caused by trauma using postnatal, tamoxifen-inducible, scleraxis-lineage restricted reporter mice (Scx-creERT2/tdTomato(fl/fl) )...
November 8, 2016: Stem Cells
https://www.readbyqxmd.com/read/27862098/simvastatin-up-regulates-annexin-a10-that-can-inhibit-the-proliferation-migration-and-invasion-in-androgen-independent-human-prostate-cancer-cells
#19
Yoshiyuki Miyazawa, Yoshitaka Sekine, Haruo Kato, Yosuke Furuya, Hidekazu Koike, Kazuhiro Suzuki
BACKGROUND: Statins have recently been studied for their proapoptotic and antimetastatic effects. However, the exact mechanisms of their anticancer actions remain unclear. Using microarrays, we discovered up-regulation of annexin A10 (ANXA10) in PC-3 cells after simvastatin treatment. ANXA10 reportedly has antitumor effects. In this study, we evaluated the effects of simvastatin on ANXA10 signaling in androgen-independent prostate cancer cells. METHODS: PC-3, LNCaP-LA (which were derived from LNCaP cells and cultured in 10% charcoal-stripped fetal bovine serum for 3 months), and DU145 human prostate cancer cell lines were used...
November 8, 2016: Prostate
https://www.readbyqxmd.com/read/27802204/knockdown-of-s100a4-blocks-growth-and-metastasis-of-anaplastic-thyroid-cancer-cells-in-vitro-and-in-vivo
#20
Kejun Zhang, Meiqin Yu, Fengyun Hao, Anbing Dong, Dong Chen
Anaplastic thyroid cancer (ATC) is a locally aggressive type of thyroid tumor with high rate of distant metastases. It is often incurable because it does not respond to radioiodine, radiotherapy, or chemotherapy. With conventional treatment, the median survival is about 6 months; therefore, new treatment options are needed. S100A4 is a calcium-binding protein related to the metastatic potential of carcinoma. Previous study has found S100A4 was overexpressed in human papillary thyroid carcinomas (PTC) tissues, and overexpression of S100A4 is associated with thyroid tumour invasion and metastasis...
September 26, 2016: Cancer Biomarkers: Section A of Disease Markers
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