keyword
MENU ▼
Read by QxMD icon Read
search

Fungal heat shock protein

keyword
https://www.readbyqxmd.com/read/28484941/proteomics-as-a-tool-to-identify-new-targets-against-aspergillus-and-scedosporium-in-the-context-of-cystic-fibrosis
#1
Andoni Ramirez-Garcia, Aize Pellon, Idoia Buldain, Aitziber Antoran, Aitana Arbizu-Delgado, Xabier Guruceaga, Aitor Rementeria, Fernando L Hernando
Cystic fibrosis (CF) is a genetic disorder that increases the risk of suffering microbial, including fungal, infections. In this paper, proteomics-based information was collated relating to secreted and cell wall proteins with potential medical applications from the most common filamentous fungi in CF, i.e., Aspergillus and Scedosporium/Lomentospora species. Among the Aspergillus fumigatus secreted allergens, β-1,3-endoglucanase, the alkaline protease 1 (Alp1/oryzin), Asp f 2, Asp f 13/15, chitinase, chitosanase, dipeptidyl-peptidase V (DppV), the metalloprotease Asp f 5, mitogillin/Asp f 1, and thioredoxin reductase receive a special mention...
May 8, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28465686/the-effect-of-fermented-lingonberry-juice-on-candida-glabrata-intracellular-protein-expression
#2
Pirjo Pärnänen, Ali Nawaz, Timo Sorsa, Jukka Meurman, Pirjo Nikula-Ijäs
Lingonberries have a long traditional use in treating fungal infections on mucosal membranes, but very little is known about the exact antifungal mechanisms. We tested the effects of fermented lingonberry juice on Candida glabrata intracellular protein expression. A Candida glabrata clinical strain was grown in the presence of fermented lingonberry juice (FLJ). Also the effect of lowered pH was tested. Intracellular protein expression levels were analyzed by the 2D-DIGE method. Six proteins detected with ≥1...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28401337/rrm-domain-of-arabidopsis-splicing-factor-sf1-is-important-for-pre-mrna-splicing-of-a-specific-set-of-genes
#3
Keh Chien Lee, Yun Hee Jang, Soon-Kap Kim, Hyo-Young Park, May Phyo Thu, Jeong Hwan Lee, Jeong-Kook Kim
The RNA recognition motif of Arabidopsis splicing factor SF1 affects the alternative splicing of FLOWERING LOCUS M pre-mRNA and a heat shock transcription factor HsfA2 pre-mRNA. Splicing factor 1 (SF1) plays a crucial role in 3' splice site recognition by binding directly to the intron branch point. Although plant SF1 proteins possess an RNA recognition motif (RRM) domain that is absent in its fungal and metazoan counterparts, the role of the RRM domain in SF1 function has not been characterized. Here, we show that the RRM domain differentially affects the full function of the Arabidopsis thaliana AtSF1 protein under different experimental conditions...
April 11, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28373280/heat-shock-protein-104-hsp104-mediated-curing-of-psi-yeast-prions-depends-on-both-psi-conformation-and-the-properties-of-the-hsp104-homologs
#4
Xiaohong Zhao, Ramon Rodriguez, Rebecca E Silberman, Joseph M Ahearn, Sheela Saidha, Kaelyn C Cummins, Daniel Masison, Evan Eisenberg, Lois E Greene
Prions arise from proteins that have two possible conformations: properly folded and non-infectious, or misfolded and infectious. The [PSI (+) ] yeast prion, which is the misfolded, self-propagating form of the translation termination factor eRF3 (Sup35), can be cured of its infectious conformation by overexpression of Hsp104, which helps dissolve the prion seeds. This dissolution depends on the trimming activity of Hsp104, which reduces the size of the prion seeds without increasing their number. To further understand the relationship between trimming and curing, trimming was followed by measuring the loss of GFP-labeled Sup35 foci from both strong and weak [PSI (+) ] variants; the former variant has more seeds and less soluble Sup35 than the latter...
April 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28219758/resolving-relationships-at-the-animal-fungal-divergence-a-molecular-phylogenetic-study-of-the-protist-trichomycetes-ichthyosporea-eccrinida
#5
Nicole K Reynolds, Matthew E Smith, Eric D Tretter, Justin Gause, Dustin Heeney, Matías J Cafaro, James F Smith, Stephen J Novak, William A Bourland, Merlin M White
Trichomycetes is a group of microorganisms that was considered a class of fungi comprising four orders of commensal, gut-dwelling endosymbionts obligately associated with arthropods. Since molecular phylogenies revealed two of those orders (Amoebidiales and Eccrinales="protist trichos") to be closely related to members of the protist class Ichthyosporea (=Mesomycetozoea), trichomycetes have been considered an ecological association of both early-diverging fungi and protists. Understanding of the taxonomy, evolution, and diversity of the protist trichos is lacking largely due to the difficulties inherent in species collection that have contributed to undersampling and understudy...
April 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28139929/chlorinated-dehydrocurvularins-and-alterperylenepoxide-a-from-alternaria-sp-ast0039-a-fungal-endophyte-of-astragalus-lentiginosus
#6
Bharat P Bashyal, E M Kithsiri Wijeratne, Joseph Tillotson, A Elizabeth Arnold, Eli Chapman, A A Leslie Gunatilaka
Investigation of Alternaria sp. AST0039, an endophytic fungus obtained from the leaf tissue of Astragalus lentiginosus, led to the isolation of (-)-(10E,15S)-4,6-dichloro-10(11)-dehydrocurvularin (1), (-)-(10E,15S)-6-chloro-10(11)-dehydrocurvularin (2), (-)-(10E,15S)-10(11)-dehydrocurvularin (3), and alterperylenepoxide A (4) together with scytalone and α-acetylorcinol. Structures of 1 and 4 were established from their spectroscopic data, and the relative configuration of 4 was determined with the help of nuclear Overhauser effect difference data...
February 24, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28056792/elucidation-of-the-molecular-responses-of-a-cucumber-segment-substitution-line-carrying-pm5-1-and-its-recurrent-parent-triggered-by-powdery-mildew-by-comparative-transcriptome-profiling
#7
Qiang Xu, Xuewen Xu, Yang Shi, Xiaohua Qi, Xuehao Chen
BACKGROUND: Powdery mildew (PM) is one of the most severe fungal diseases of cucurbits, but the molecular mechanisms underlying PM resistance in cucumber remain elusive. In this study, we developed a PM resistant segment substitution line SSL508-28 that carried a segment on chromosome five representing the Pm5.1 locus from PM resistant donor Jin5-508 using marker-assisted backcrossing of an elite PM susceptible cucumber inbred line D8. RESULTS: Whole-genome resequencing of SSL508-28, Jin5-508 and D8 was performed to identify the exact boundaries of the breakpoints for this introgression because of the low density of available single sequence repeat markers...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/27876624/differences-in-structure-and-changes-in-gene-regulation-of-murrel-molecular-chaperone-hsp-family-during-epizootic-ulcerative-syndrome-eus-infection
#8
Akila Sathyamoorthy, Mukesh Kumar Chaurasia, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ramasamy Harikrishnan, Jesu Arockiaraj
Heat shock proteins (HSPs) are immunogenic, ubiquitous class of molecular chaperones, which are induced in response to various environmental and microbial stressful conditions. It plays a vital role in maintaining cellular protein homeostasis in eukaryotic cells. In this study, we described a comprehensive comparative data by bioinformatics approach on three different full length cDNA sequences of HSP family at molecular level. The cDNA sequences of three HSPs were identified from constructed cDNA library of Channa striatus and named as CsCPN60, CsHSP60 and CsHSP70...
January 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27867390/the-mechanisms-of-maize-resistance-to-fusarium-verticillioides-by-comprehensive-analysis-of-rna-seq-data
#9
Yanping Wang, Zijian Zhou, Jingyang Gao, Yabin Wu, Zongliang Xia, Huiyong Zhang, Jianyu Wu
Fusarium verticillioides is the most commonly reported fungal species responsible for ear rot of maize which substantially reduces grain yield. It also results in a substantial accumulation of mycotoxins that give rise to toxic response when ingested by animals and humans. For inefficient control by chemical and agronomic measures, it thus becomes more desirable to select more resistant varieties. However, the molecular mechanisms underlying the infection process remain poorly understood, which hampers the application of quantitative resistance in breeding programs...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27789295/effect-of-stress-on-heat-shock-protein-levels-immune-response-and-survival-to-fungal-infection-of-mamestra-brassicae-larvae
#10
E H Richards, M P Dani, Y Lu, T Butt, R J Weaver
Although the utilisation of fungal biological control agents to kill insect pests is desirable, it is known that the outcome of infection may be influenced by a number of criteria, including whether or not the target insect is stressed. In the current work, topical treatment of larvae of the lepidopteran pest, Mamestra brassicae, with conidia of Beauveria bassiana, followed by a heat stress (HS; 37°C for 1h) 48h later, resulted in a similar level of larval survival to that occurring for no heat stress (No-HS), fungus-treated larvae...
January 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27743033/heat-shock-protein-83-plays-pleiotropic-roles-in-embryogenesis-longevity-and-fecundity-of-the-pea-aphid-acyrthosiphon-pisum
#11
Torsten Will, Henrike Schmidtberg, Marisa Skaljac, Andreas Vilcinskas
Heat shock protein 83 (HSP83) is homologous to the chaperone HSP90. It has pleiotropic functions in Drosophila melanogaster, including the control of longevity and fecundity, and facilitates morphological evolution by buffering cryptic deleterious mutations in wild populations. In the pea aphid Acyrthosiphon pisum, HSP83 expression is moderately induced by bacterial infection but upregulated more strongly in response to heat stress and fungal infection. Stress-inducible heat shock proteins are of considerable evolutionary and ecological importance because they are known to buffer environmental variation and to influence fitness under non-optimal conditions...
January 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/27627033/identification-transcriptional-and-functional-analysis-of-heat-shock-protein-90s-in-banana-musa-acuminata-l-highlight-their-novel-role-in-melatonin-mediated-plant-response-to-fusarium-wilt
#12
Yunxie Wei, Wei Hu, Qiannan Wang, Hongqiu Zeng, Xiaolin Li, Yu Yan, Russel J Reiter, Chaozu He, Haitao Shi
As one popular fresh fruit, banana (Musa acuminata) is cultivated in the world's subtropical and tropical areas. In recent years, pathogen Fusarium oxysporum f. sp. cubense (Foc) has been widely and rapidly spread to banana cultivated areas, causing substantial yield loss. However, the molecular mechanism of banana response to Foc remains unclear, and functional identification of disease-related genes is also very limited. In this study, nine 90 kDa heat-shock proteins (HSP90s) were genomewide identified. Moreover, the expression profile of them in different organs, developmental stages, and in response to abiotic and fungal pathogen Foc were systematically analyzed...
January 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/27598463/detrimental-effect-of-fungal-60-kda-heat-shock-protein-on-experimental-paracoccidioides-brasiliensis-infection
#13
Fabrício Freitas Fernandes, Leandro Licursi de Oliveira, Taise Natali Landgraf, Gabriela Peron, Marcelo Vieira Costa, Arlete A M Coelho-Castelo, Vânia L D Bonato, Maria-Cristina Roque-Barreira, Ademilson Panunto-Castelo
The genus Paracoccidioides comprises species of dimorphic fungi that cause paracoccidioidomycosis (PCM), a systemic disease prevalent in Latin America. Here, we investigated whether administration of native 60-kDa heat shock protein of P. brasiliensis (nPbHsp60) or its recombinant counterpart (rPbHsp60) affected the course of experimental PCM. Mice were subcutaneously injected with nPbHsp60 or rPbHsp60 emulsified in complete's Freund Adjuvant (CFA) at three weeks after intravenous injection of P. brasiliensis yeasts...
2016: PloS One
https://www.readbyqxmd.com/read/27590362/pathogenesis-of-dermatophytosis-sensing-the-host-tissue
#14
REVIEW
Nilce M Martinez-Rossi, Nalu T A Peres, Antonio Rossi
The genera Trichophyton, Microsporum, and Epidermophyton include filamentous fungi that cause dermatophytosis, a superficial infection of the skin, stratum corneum, nail beds, and hair follicles. The ability of dermatophytes to adhere to these substrates and adapt to the host environment is essential for the establishment of infection. Several fungal enzymes and proteins participate in this adaptive response to the environment and to keratin degradation. Transcription factors such as PacC and Hfs1, as well as heat shock proteins, are involved in sensing and adapting to the acidic pH of the skin in the early stages of fungal-host interaction...
February 2017: Mycopathologia
https://www.readbyqxmd.com/read/27473618/cd-induced-production-of-glomalin-by-arbuscular-mycorrhizal-fungus-rhizophagus-irregularis-as-estimated-by-monoclonal-antibody-assay
#15
Elham Malekzadeh, Nasser Aliasgharzad, Jafar Majidi, Leili Aghebati-Maleki, Jalal Abdolalizadeh
Glomalin is a specific fungal glycoprotein produced by arbuscular mycorrhizal (AM) fungi belonging to the Glomerales which could efficiently sequestrate heavy metals. The glomalin has been introduced as a heat shock protein and there are evidences that increasing levels of heavy metals could enhance its production. We examined the influence of Cd concentrations on glomalin production by AM fungus, as well as its contribution to the sequestration of Cd in both pot and in vitro culture conditions. Pot experiment was carried out using pure sand with Trifolium repens L...
October 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27306495/heat-shock-protein-90-is-required-for-sexual-and-asexual-development-virulence-and-heat-shock-response-in-fusarium-graminearum
#16
Duc-Cuong Bui, Yoonji Lee, Jae Yun Lim, Minmin Fu, Jin-Cheol Kim, Gyung Ja Choi, Hokyoung Son, Yin-Won Lee
Eukaryotic cells repress global translation and selectively upregulate stress response proteins by altering multiple steps in gene expression. In this study, genome-wide transcriptome analysis of cellular adaptation to thermal stress was performed on the plant pathogenic fungus Fusarium graminearum. The results revealed that profound alterations in gene expression were required for heat shock responses in F. graminearum. Among these proteins, heat shock protein 90 (FgHsp90) was revealed to play a central role in heat shock stress responses in this fungus...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27303729/antibody-binding-alters-the-characteristics-and-contents-of-extracellular-vesicles-released-by-histoplasma-capsulatum
#17
Ludmila Matos Baltazar, Ernesto S Nakayasu, Tiago J P Sobreira, Hyungwon Choi, Arturo Casadevall, Leonardo Nimrichter, Joshua D Nosanchuk
Histoplasma capsulatum produces extracellular vesicles containing virulence-associated molecules capable of modulating host machinery, benefiting the pathogen. Treatment of H. capsulatum cells with monoclonal antibodies (MAbs) can change the outcome of infection in mice. We evaluated the sizes, enzymatic contents, and proteomic profiles of the vesicles released by fungal cells treated with either protective MAb 6B7 (IgG1) or nonprotective MAb 7B6 (IgG2b), both of which bind H. capsulatum heat shock protein 60 (Hsp60)...
March 2016: MSphere
https://www.readbyqxmd.com/read/27193371/heterologous-expression-of-the-hsp24-from-trichoderma-asperellum-improves-antifungal-ability-of-populus-transformant-pdpap-hsp24%C3%A2-s-to-cytospora-chrysosperma-and-alternaria-alternate
#18
S D Ji, Z Y Wang, H J Fan, R S Zhang, Z Y Yu, J J Wang, Z H Liu
The tolerance of plants to biotic and abiotic stresses could be improved by transforming with fungal resistance-related genes. In this study, the cDNA sequence (GenBank Acc. No. KP337939) of the resistance-related gene Hsp24 encoding the 24 kD heat shock protein was obtained from the biocontrol fungus Trichoderma asperellum ACCC30536. The promoter region of Hsp24 contained many cis-regulators related to stresses response, such as "GCN4" and "GCR1" etc. Hsp24 transcription in T. asperellum was up-regulated under six different environmental stresses, compared with the control...
September 2016: Journal of Plant Research
https://www.readbyqxmd.com/read/27035559/a-comparative-study-of-the-timecourse-of-the-expression-of-the-thermo%C3%A2-inducible-hsp70-gene-in-clinical-and-environmental-isolates-of-aspergillus-fumigatus
#19
COMPARATIVE STUDY
Golnaz Sharafi, Ali Reza Khosravi, Ghasem Vahedi, Ramak Yahyaraeyat, Teimur Abbasi
The internal environment within animals or humans provides different conditions to invading saprophytic fungal pathogens, requiring the differential regulation of genes in comparison to environmental conditions. Understanding the mechanisms by which pathogens regulate genes within the host may be key in determining pathogen behavior within the host and may additionally facilitate further investigation into novel therapeutic agents. The heat shock protein (HSP)70 gene and its associated proteins have been frequently reported to be among the most highly expressed and dominant proteins present within various locations at physiological temperatures...
May 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26714790/mas5-a-homologue-of-bacterial-dnaj-is-indispensable-for-the-host-infection-and-environmental-adaptation-of-a-filamentous-fungal-insect-pathogen
#20
Jie Wang, Sheng-Hua Ying, Yue Hu, Ming-Guang Feng
Mas5, a yeast heat shock protein classified to the Hsp40 group, is homologous to bacterial archetype DnaJ but functionally unexplored in filamentous fungi. Here we identify a Mas5 homologue (46.86 kDa) in Beauveria bassiana and show its indispensability for host infection and environmental adaptation of the fungal insect pathogen. The deletion of mas5 caused severe defects in aerial conidiation, conidial germination and submerged blastospore production (mimic to host haemocoel). The deletion mutant lost 100% virulence to Galleria mellonella larvae through normal cuticular penetration (topical inoculation) and 50% through cuticle-bypassing infection (intrahaemocoel injection)...
March 2016: Environmental Microbiology
keyword
keyword
38069
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"