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Fungal HSP

Wilfried Posch, Michael Blatzer, Doris Wilflingseder, Cornelia Lass-Flörl
The polyene antifungal amphotericin B (AmB) exerts a powerful and broad activity against a vast array of fungi and in general displays a remarkably low rate of antimicrobial resistance. Aspergillus terreus holds an exceptional position among the Aspergilli due to its intrinsic AmB resistance, in vivo and in vitro. Until now, the underlying mechanisms of polyene resistance were not well understood. This review will highlight the molecular basis of A. terreus and AmB resistance recently gained and will display novel data on the mode of action of AmB...
April 1, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Akila Sathyamoorthy, Mukesh Kumar Chaurasia, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Ramasamy Harikrishnan, Jesu Arockiaraj
Heat shock proteins (HSPs) are immunogenic, ubiquitous class of molecular chaperones, which are induced in response to various environmental and microbial stressful conditions. It plays a vital role in maintaining cellular protein homeostasis in eukaryotic cells. In this study, we described a comprehensive comparative data by bioinformatics approach on three different full length cDNA sequences of HSP family at molecular level. The cDNA sequences of three HSPs were identified from constructed cDNA library of Channa striatus and named as CsCPN60, CsHSP60 and CsHSP70...
January 2017: Fish & Shellfish Immunology
E H Richards, M P Dani, Y Lu, T Butt, R J Weaver
Although the utilisation of fungal biological control agents to kill insect pests is desirable, it is known that the outcome of infection may be influenced by a number of criteria, including whether or not the target insect is stressed. In the current work, topical treatment of larvae of the lepidopteran pest, Mamestra brassicae, with conidia of Beauveria bassiana, followed by a heat stress (HS; 37°C for 1h) 48h later, resulted in a similar level of larval survival to that occurring for no heat stress (No-HS), fungus-treated larvae...
January 2017: Journal of Insect Physiology
Golnaz Sharafi, Ali Reza Khosravi, Ghasem Vahedi, Ramak Yahyaraeyat, Teimur Abbasi
The internal environment within animals or humans provides different conditions to invading saprophytic fungal pathogens, requiring the differential regulation of genes in comparison to environmental conditions. Understanding the mechanisms by which pathogens regulate genes within the host may be key in determining pathogen behavior within the host and may additionally facilitate further investigation into novel therapeutic agents. The heat shock protein (HSP)70 gene and its associated proteins have been frequently reported to be among the most highly expressed and dominant proteins present within various locations at physiological temperatures...
May 2016: Molecular Medicine Reports
Seema D Shekhawat, Hemant J Purohit, Girdhar M Taori, Hatim F Daginawala, Rajpal S Kashyap
OBJECTIVES: Diagnosis of tuberculosis meningitis (TBM) remains challenging in tuberculosis (TB) endemic countries. The need for TB biomarkers arises, in part, from the difficulty of accurately diagnosing TBM with the available methods. PATIENTS AND METHODS: To explore the potential of host Hsps (Hsp 25, Hsp 60, Hsp 70 and Hsp 90) as an alternative marker in TBM diagnosis, we evaluated cerebrospinal fluid (CSF) sample of TBM (n=49), Pyogenic Meningitis (PM) (n=20), Viral Meningitis (VM) (n=09), Fungal Meningitis (FM) (n=04) and non infectious control (n=79) patients using indirect ELISA...
January 2016: Clinical Neurology and Neurosurgery
Tiago R Jacob, Nalu T A Peres, Maíra P Martins, Elza A S Lang, Pablo R Sanches, Antonio Rossi, Nilce M Martinez-Rossi
Treatment of fungal infections is difficult due to several reasons, such as side effects of drugs, emergence of resistant strains, and limited number of molecular targets for the drug compounds. In fungi, heat shock proteins (Hsps) have been implicated in several processes with the conserved molecular chaperone Hsp90 emerging as a potential target for antifungal therapy. It plays key cellular roles by eliciting molecular response to environmental changes, morphogenesis, antifungal resistance, and fungal pathogenicity...
2015: Frontiers in Microbiology
Coralia Bleotu, Mariana Carmen Chifiriuc, Gratiela Pircalabioru, Şerban Vifor Gabriel Berteşteanu, Raluca Grigore, Simona Maria Ruta, Veronica Lazar
Heat shock proteins (HSP) represent important antigenic targets for the immune response, playing an important role in the pathology and infectious diseases control. The purpose of this work was to investigate the levels of HSP60 and HSP70 specific antibodies in the bloodstream of patients with different bacterial infections and cancer, in order to evaluate their potential role as diagnosis markers of different infectious diseases. Detection of specific anti-HSP 60 and HSP 70 serum levels was performed by ELISA...
2014: Virulence
Michelle D Leach, Leah E Cowen
Temperature is a ubiquitous environmental variable which can profoundly influence the physiology of living cells as it changes over time and space. When yeast cells are exposed to a sublethal heat shock, normal metabolic functions become repressed and the heat shock transcription factor Hsf1 is activated, inducing heat shock proteins (HSPs). Candida albicans, the most prevalent human fungal pathogen, is an opportunistic pathogen that has evolved as a relatively harmless commensal of healthy individuals. Even though C...
August 2014: Eukaryotic Cell
Carolina P Silveira, Alicia C Piffer, Lívia Kmetzsch, Fernanda L Fonseca, Danielle A Soares, Charley C Staats, Marcio L Rodrigues, Augusto Schrank, Marilene H Vainstein
The pathogenic yeast Cryptococcus neoformans secretes numerous proteins, such as heat shock proteins, by unconventional mechanisms during its interaction with host cells. Hsp70 is a conserved chaperone that plays important roles in various cellular processes, including the interaction of fungi with host immune cells. Here, we report that sera from individuals with cryptococcosis infection recognize a recombinant C. neoformans Hsp70 (Cn_rHsp70). Moreover, immunofluorescence assays using antibodies against Cn_rHsp70 revealed the localization of this protein at the cell surface mainly in association with the capsular network...
November 2013: Fungal Genetics and Biology: FG & B
Agata Piecuch, Ewa Obłąk
Changes in environmental conditions might be a stress factor for yeast cells. There are several mechanisms of stress tolerance, developed by the cell, which activate when the stress appears. Different transcription factors coordinate the expression of stress response genes. Msn2/4p regulate the expression of the general stress response. Heat shock defense involves heat shock proteins (Hsp), controlled by Hsf1p. Osmotic shock induces the MAP kinase cascade (HOG), whereas the oxidative stress response requires the YAP network...
2013: Postȩpy Higieny i Medycyny Doświadczalnej
Jana Piterková, Lenka Luhová, Barbora Mieslerová, Aleš Lebeda, Marek Petřivalský
Heat shock proteins (HSP) are produced in response to various stress stimuli to prevent cell damage. We evaluated the involvement of nitric oxide (NO) and reactive oxygen species (ROS) in the accumulation of Hsp70 proteins in tomato leaves induced by abiotic and biotic stress stimuli. A model system of leaf discs was used with two tomato genotypes, Solanum lycopersicum cv. Amateur and Solanum chmielewskii, differing in their resistance to fungal pathogen Oidium neolycopersici. Leaf discs were exposed to stress factors as heat shock and pathogen infection alone or in a combination, and treated with substances modulating endogenous NO and ROS levels...
June 2013: Plant Science: An International Journal of Experimental Plant Biology
Sunyoung Kim, David S Gross
The evolutionarily conserved Mediator complex is central to the regulation of gene transcription in eukaryotes because it serves as a physical and functional interface between upstream regulators and the Pol II transcriptional machinery. Nonetheless, its role appears to be context-dependent, and the detailed mechanism by which it governs the expression of most genes remains unknown. Here we investigate Mediator involvement in HSP (heat shock protein) gene regulation in the yeast Saccharomyces cerevisiae. We find that in response to thermal upshift, subunits representative of each of the four Mediator modules (Head, Middle, Tail, and Kinase) are rapidly, robustly, and selectively recruited to the promoter regions of HSP genes...
April 26, 2013: Journal of Biological Chemistry
Rashmi Aggarwal, Sangeeta Gupta, Sapna Sharma, Sagar Banerjee, Priyanka Singh
The present study reports molecular characterization of small heat shock protein gene in Indian isolates of Chaetomium globosum, C. perlucidum, C. reflexum, C. cochlioides and C. cupreum. Six isolates of C. globosum and other species showed a band of 630bp using specific primers. Amplified cDNA product of C. globosum (Cg 1) cloned and sequenced showed 603bp open reading frame encoding 200 amino-acids. The protein sequence had a molecular mass of 22 kDa and was therefore, named Hsp22. BlastX analysis revealed that the gene codes for a protein homologous to previously characterized Hsp22...
November 2012: Indian Journal of Experimental Biology
Michelle D Leach, Susan Budge, Louise Walker, Carol Munro, Leah E Cowen, Alistair J P Brown
Thermal adaptation is essential in all organisms. In yeasts, the heat shock response is commanded by the heat shock transcription factor Hsf1. Here we have integrated unbiased genetic screens with directed molecular dissection to demonstrate that multiple signalling cascades contribute to thermal adaptation in the pathogenic yeast Candida albicans. We show that the molecular chaperone heat shock protein 90 (Hsp90) interacts with and down-regulates Hsf1 thereby modulating short term thermal adaptation. In the longer term, thermal adaptation depends on key MAP kinase signalling pathways that are associated with cell wall remodelling: the Hog1, Mkc1 and Cek1 pathways...
December 2012: PLoS Pathogens
Hianna Almeida Câmara Leite, Anderson Barbosa Silva, Fábio Pinto Gomes, Karina Peres Gramacho, José Cláudio Faria, Jorge Teodoro de Souza, Leandro Lopes Loguercio
Clonal genotypes resistant to fungal diseases are an important component of the cocoa production system in southeastern Bahia state (Brazil), so that technologies for faster production of stronger and healthier plantlets are highly desirable. In this study, the effects of inoculated bacterial endophytes isolated from healthy adult cacao plants on seedlings, and aspects related to inoculation methods, colonization patterns, and photosynthesis were investigated. Sequencing of 16S rRNA, hsp-60, and rpo-B genes placed the wild-type isolates within the species Enterobacter cloacae (isolates 341 and 344) and Bacillus subtilis (isolate 629)...
March 2013: Applied Microbiology and Biotechnology
K Papamichael, B Delitheos, E Tiligada
AIMS: Histamine interacts with the stress response in eukaryotes. This study investigated the effects of antihistamines on the heat shock (HS) response in yeast, thereby exploring their functions in a well-established histamine receptor (H(x) R)-free model. METHODS AND RESULTS: Stress response was evaluated by determining growth and viability of postlogarithmic phase grown yeast cultures after HS at 53°C for 30 min. The effects of H(x) R ligands were investigated following short- and long-term administration...
February 2013: Journal of Applied Microbiology
Shigang Gao, Tong Liu, Yingying Li, Qiong Wu, Kehe Fu, Jie Chen
Curvularia lunata is an important pathogen causing Curvularia leaf spot in maize. Significant pathogenic variation has been found in C. lunata. To better understand the mechanism of this phenomenon, we consecutively put the selective pressures of resistant maize population on C. lunata strain WS18 (low virulence) artificially. As a result, the virulence of this strain was significantly enhanced. Using 2DE, 12 up-regulated and four down-regulated proteins were identified in virulence-increased strain compared to WS18...
December 2012: Proteomics
Younes Ghasemi, Fatemeh Dabbagh, Sara Rasoul-Amini, Afshin Borhani Haghighi, Mohammad Hossein Morowvat
Current evidence lends increasing support to immunoinflammatory mechanisms as one of the prime pathogenic processes involved in the development and progression of Behçet's disease (BD). It has been observed that most human beings have cellular and humoral reactions against microbial heat shock proteins (HSPs). The observation that eukaryotic and prokaryotic HSPs have high sequence similarity promoted the hypothesis that HSPs might be potential candidates for molecular mimicry and could act as potentially dangerous autoantigens...
November 2012: Computers in Biology and Medicine
Todd Lamitina, Eric Chevet
The fungal pathogen Drechmeria coniospora infects C. elegans and elicits an innate immune response mediated, in part, by the induction of antimicrobial peptides in the epidermis. The signaling pathways controlling this phenomenon remain to be fully characterized. In this issue of Virulence, Couillault and colleagues use both proteomics and genetics to discover an unexpected role for the unfolded protein response (UPR) target chaperone BiP/GRP78/hsp-3 in the control of fungal infection-induced antimicrobial peptide expression in C...
May 1, 2012: Virulence
Choowong Auesukaree, Preeyaporn Koedrith, Pornpon Saenpayavai, Thipa Asvarak, Suthee Benjaphokee, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima, Chuenchit Boonchird
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723, C3751 and C3867) from Thai fruits were capable of growing and producing 38 g/L ethanol up to 41°C. Based on genetic analyses, these isolates were prototrophic and homothallic, with dominant homothallic and thermotolerant phenotypes...
August 2012: Journal of Bioscience and Bioengineering
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