keyword
MENU ▼
Read by QxMD icon Read
search

Urothelium

keyword
https://www.readbyqxmd.com/read/28188758/a-role-for-the-hedgehog-effector-gli1-in-mediating-stent-induced-ureteral-smooth-muscle-dysfunction-and-aperistalsis
#1
Claudia Janssen, Ralph Buttyan, Chun Y Seow, Wolfgang Jäger, Dennis Solomon, Ladan Fazli, Ben H Chew, Dirk Lange
OBJECTIVES: To better understand the effects of Double J (JJ) stenting on ureteral physiology and function. MATERIALS AND METHODS: In total, twenty-four pigs (24) were stented cystoscopically unilaterally for 48h, 1, 2, 4 and 7 wks. Controls consisted of un-stented animals (n=4) or the contralateral un-stented ureter in pigs. Ureters were harvested and tested in tissue baths to evaluate their contractility. Ureteral inflammation and expression of Sonic Hedgehog (Shh) and the transcriptional activator Gli1 (the downstream target of active HH signaling) were assessed histologically and by immunohistochemistry, respectively...
February 8, 2017: Urology
https://www.readbyqxmd.com/read/28165151/horseradish-extract-promotes-urinary-bladder-carcinogenesis-when-administered-to-f344-rats-in-drinking-water
#2
Young-Man Cho, Mai Hasumura, Toshio Imai, Shigeaki Takami, Akiyoshi Nishikawa, Kumiko Ogawa
Horseradish extract (HRE), consisting mainly of a mixture of allyl isothiocyanate and other isothiocyanates, has been used as a food additive. To evaluate the potential hazards of HRE, a 104-week chronic study, a 2-week analysis of cell proliferation in the urinary bladder and a medium-term promotion bioassay of HRE were conducted with administration at concentrations of up to 0.04% HRE in the drinking water to male F344 rats. In the 104-week chronic study with 32 male rats per group, no treatment-related increases in the incidences of neoplastic lesions in any organ, including urinary bladder, were observed, except for simple hyperplasia in the urinary bladder in rats treated with HRE at concentrations of more than 0...
February 6, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28163793/uromark-a-urinary-biomarker-assay-for-the-detection-of-bladder-cancer
#3
Andrew Feber, Pawan Dhami, Liqin Dong, Patricia de Winter, Wei Shen Tan, Mónica Martínez-Fernández, Dirk S Paul, Antony Hynes-Allen, Sheida Rezaee, Pratik Gurung, Simon Rodney, Ahmed Mehmood, Felipe Villacampa, Federico de la Rosa, Charles Jameson, Kar Keung Cheng, Maurice P Zeegers, Richard T Bryan, Nicholas D James, Jesus M Paramio, Alex Freeman, Stephan Beck, John D Kelly
BACKGROUND: Bladder cancer (BC) is one of the most common cancers in the western world and ranks as the most expensive to manage, due to the need for cystoscopic examination. BC shows frequent changes in DNA methylation, and several studies have shown the potential utility of urinary biomarkers by detecting epigenetic alterations in voided urine. The aim of this study is to develop a targeted bisulfite next-generation sequencing assay to diagnose BC from urine with high sensitivity and specificity...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28156053/urothelial-cell-expansion-and-differentiation-are-improved-by-exposure-to-hypoxia
#4
Stéphane Chabaud, Ingrid Saba, Clément Baratange, Brice Boiroux, Maude Leclerc, Alexandre Rousseau, Sara Bouhout, Stéphane Bolduc
Cells obtained from a patient's biopsy have to be expanded after extraction to produce autologous tissues, but standard cell culture conditions often limit their growth or lifespan and could induce early and inadequate cell differentiation. Moreover, it has previously been reported that the air-liquid interface, that induces maturation of the urothelium, stimulated inadequate differentiation associated with aberrant keratin-14 expression. The aim of this study was to test the benefits of hypoxia during expansion of urothelial cells and maturation of the bladder epithelium in the context of tissue engineering...
February 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28149932/aquaporin-3-expression-loss-in-urothelial-carcinoma-association-with-tumor-invasion-depth-but-not-with-grading
#5
Johannes Breyer, Wolfgang Otto, Maximilian Burger, Arndt Hartmann, Peter C Rubenwolf
We have previously provided molecular evidence of expression of aquaporin 3 (AQP3) in normal human urothelium and in UBC of various stages. Whereas former studies demonstrated that loss of AQP3 was associated with invasive and high-grade disease and worse progression-free and cancer-specific survival, this report investigates the expression of AQP3 in associated CIS. Contrary to what we had expected, all CIS specimens were shown to exhibit strong AQP3 expression, suggesting loss of AQP3 in UBC is primarily associated with the ability of tumor cells for invasion but not with grading as sign of dedifferentiation...
January 27, 2017: Bladder Cancer
https://www.readbyqxmd.com/read/28135607/hierarchical-transcriptional-profile-of-urothelial-cells-development-and-differentiation
#6
REVIEW
Ban Al-Kurdi
The urothelial lining of the lower urinary tract is the most efficient permeability barrier in animals, exhibiting a highly differentiated phenotype and a remarkable regenerative capacity upon wounding. During development and possibly during repair, cells undergo a sequence of hierarchical transcriptional events that mark the transition of these cells from the least differentiated urothelial phenotype characteristic of the basal cell layer, to the most differentiated cellular phenotype characteristic of the superficial cell layer...
January 27, 2017: Differentiation; Research in Biological Diversity
https://www.readbyqxmd.com/read/28122714/sleeping-beauty-awakening-urothelium-from-its-slumber
#7
Zarine R Balsara, Xue Li
The bladder urothelium is essentially quiescent but regenerates readily upon injury. The process of urothelial regeneration harkens back to the process of urothelial development whereby urothelial stem/progenitor cells must proliferate and terminally differentiate to establish all three urothelial layers. How the urothelium regulates the level of proliferation and the timing of differentiation to ensure the precise degree of regeneration is of significant interest in the field. Without a carefully-orchestrated process, urothelial regeneration may be inadequate, thereby exposing the host to toxins or pathogens, or excessive, thereby setting the stage for tumor development...
January 25, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28119486/repeated-treatments-with-chitosan-in-combination-with-antibiotics-completely-eradicate-uropathogenic-escherichia-coli-from-infected-mouse-urinary-bladders
#8
Andreja Erman, Veronika Križan Hergouth, Matthew G Blango, Mojca Kerec Kos, Matthew A Mulvey, Peter Veranič
Uropathogenic Escherichia coli (UPEC), the primary causative agent of urinary tract infections, colonize and invade the epithelial cells of the bladder urothelium. Infection of immature urothelial cells can result in the formation of persistent intracellular reservoirs that are refractory to antibiotic treatments. Previously, we defined a novel therapeutic strategy utilizing the bladder cell exfoliant chitosan to deplete UPEC reservoirs. However, though a single treatment of chitosan followed by ciprofloxacin administration had a marked effect on reducing UPEC titers within the bladder, this treatment failed to prevent relapsing bacteriuria...
January 24, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28109014/urothelial-generation-and-regeneration-in-development-injury-and-cancer
#9
REVIEW
Caihong Wang, Whitney Trotter Ross, Indira U Mysorekar
Homeostatic maintenance and repair of the urothelium upon injury are required for a functional bladder in both healthy and disease conditions. Understanding the cellular and molecular mechanisms underlying the urothelial regenerative response is key to designing strategies for tissue repair and ultimately treatments for urologic diseases including urinary tract infections, voiding dysfunction, painful bladder syndrome and bladder cancer. In this article, we review studies on urothelial ontogeny during development and regeneration following various injury modalities...
January 20, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28106785/ketamine-analog-methoxetamine-induced-inflammation-and-dysfunction-of-bladder-in-rats
#10
Qiang Wang, Qinghui Wu, Junpeng Wang, Yang Chen, Guihao Zhang, Jiawei Chen, Jie Zhao, Peng Wu
The novel synthetic psychoactive ketamine analog methoxetamine is reportedly being used for recreational purposes. As ketamine use can result in urinary dysfunction, we conducted the present study to investigate how methoxetamine affects the bladder. A cystometry investigation showed that female Sprague-Dawley rats experienced increased micturition frequency bladder dysfunction after receiving a daily intraperitoneal injection of 30 mg/kg methoxetamine or ketamine for periods of 4 or 12 weeks. Histologic examinations of rat bladder tissue revealed damaged urothelium barriers, as well as evidence of inflammatory cell infiltration and matrix deposition...
January 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28101348/evans-blue-mediated-white-light-detection-of-non-muscle-invasive-bladder-cancer-a-preclinical-feasibility-and-safety-study-using-a-rat-bladder-urothelial-cell-carcinoma-model
#11
Sanne Elsen, Evelyne Lerut, Frank Van Der Aa, Ben Van Cleynenbreugel, Hendrik Van Poppel, Peter De Witte
Photodynamic diagnosis (PDD) improves the detection of non-muscle-invasive bladder cancer (NMIBC). However, white-light (WL) cystoscopy remains the technique routinely used in urological clinics. A more cost-effective but equally performant alternative to PDD may encompass the use of an intense tumoritropic dye in combination with WL cystoscopy. Using a preclinical setting, we investigated the practical aspects of the use of Evans blue (EB) dye for the possible future detection of NMIBC using WL cystoscopy...
December 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/28098864/victor-vinflunine-in-advanced-metastatic-transitional-cell-carcinoma-of-the-urothelium-a-retrospective-analysis-of-the-use-of-vinflunine-in-multi-centre-real-life-setting-as-second-line-chemotherapy-through-free-of-charge-programme-for-patients-in-the-uk-and
#12
Syed A Hussain, Jawaher Ansari, Robert Huddart, Derek G Power, Jeanette Lyons, James Wylie, Maria Vilarino-Varlela, Nils O Elander, Rhona McMenemin, Lisa M Pickering, Guy Faust, Seema Chauhan, Richard J Jackson
There is no standard of care in the UK or Ireland for second-line chemotherapy for patients with advanced transitional cell carcinoma (TCCU). Vinflunine is approved for TCCU patients who have failed a platinum-based regimen, and is standard of care in Europe but is not routinely available in the UK. Data were collected retrospectively on patients who received vinfluine as a second-line treatment. The aims were to document the toxicity and efficacy in a real life setting. Data were collected on 49 patients from 9 sites across the UK and Ireland [median age, 64 (IQR, 57-70) years, 33 males]...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28087925/host-responses-to-urinary-tract-infections-and-emerging-therapeutics-sensation-and-pain-within-the-urinary-tract
#13
Lori A Birder, David J Klumpp
Urinary tract infection (UTI) pathogenesis is understood increasingly at the level of the uropathogens and the cellular and molecular mediators of host inflammatory responses. However, little is known about the mediators of symptoms during UTI and what distinguishes symptomatic events from asymptomatic bacteriuria. Here, we review bladder physiology and sensory pathways in the context of an emerging literature from murine models dissecting the host and pathogen factors mediating pain responses during UTI. The bladder urothelium is considered a mediator of sensory responses and appears to play a role in UTI pain responses...
October 2016: Microbiology Spectrum
https://www.readbyqxmd.com/read/28081549/microrna-promoter-methylation-a-new-tool-for-accurate-detection-of-urothelial-carcinoma
#14
Nuno André Padrão, Sara Monteiro-Reis, Jorge Torres-Ferreira, Luís Antunes, Luís Leça, Diana Montezuma, João Ramalho-Carvalho, Paula C Dias, Paula Monteiro, Jorge Oliveira, Rui Henrique, Carmen Jerónimo
BACKGROUND: Urothelial carcinoma (UC) is the most common cancer affecting the urinary system, worldwide. Lack of accurate early detection tools entails delayed diagnosis, precluding more efficient and timely treatment. In a previous study, we found that miR-129-2 and miR-663a were differentially methylated in UC compared with other genitourinary tract malignancies. Here, we evaluated the diagnostic performance of those microRNAs in urine. METHODS: Promoter methylation levels of miR-129-2 and miR-663a were assessed, using real-time quantitative methylation-specific PCR, in UC tissue samples (using normal urothelium as control) and, subsequently, in urine samples from UC and other genitourinary malignancies...
January 12, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28074261/perioperative-chemotherapy-in-upper-tract-urothelial-carcinoma-a-comprehensive-review
#15
REVIEW
Atiqullah Aziz, Jakub Dobruch, Kees Hendricksen, Luis A Kluth, Andrea Necchi, Aidan Noon, Michael Rink, Florian Roghmann, Roland Seiler, Paolo Gontero, Wassim Kassouf, Shahrokh F Shariat, Evanguelos Xylinas
PURPOSE: To evaluate the role of neoadjuvant (NAC) and adjuvant chemotherapy (AC) in patients with upper tract urothelial carcinoma (UTUC) treated with radical nephroureterectomy (RNU). METHODS: A comprehensive review of the current literature was performed searching for all studies investigating NAC and AC in UTUC in MEDLINE and https://clinicaltrials.gov , prior to April 2016. The following keywords were used: "ureteral neoplasms," "urothelium," "ureter," "upper tract urothelial," "chemotherapy," "adjuvant," "neoadjuvant" and relevant variants...
January 10, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/28050435/mucinous-cystadenocarcinoma-in-a-horse-shoe-kidney-masquerading-as-giant-hydronephrosis-a-case-report-diagnostic-challenges-lessons-learnt-and-review-of-literature
#16
Leena Dennis Joseph, Sriram Krishnamoorthy, Rajendiran Swaminathan, Gokul Kripesh, Hariharasudhan Sekar
Primary epithelial tumour of the renal pelvis is a rare entity. So far, very few cases are reported in literature. Of these, mucin secreting adenocarcinomas are distinctly rare. However, mucinous cystadenocarcinoma, that too arising in a horse shoe kidney is extremely rare to be reported. Mucinous cystadenomas and carcinomas arising from the renal pelvis are regarded to be secondary to a metaplastic change in the urothelium. Here, we present a case of a 51-year-old male, who complained of abdominal pain, palpable abdominal mass, haematuria along with passage of mucoid material in the urine, with a very poorly functioning kidney...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28049658/stage-and-subunit-specific-functions-of-polycomb-repressive-complex-2-in-bladder-urothelial-formation-and-regeneration
#17
Chunming Guo, Zarine R Balsara, Warren G Hill, Xue Li
Urothelium is the protective lining of the urinary tract. The mechanisms underlying urothelial formation and maintenance are largely unknown. Here, we report the stage-specific roles of PRC2 epigenetic regulators in embryonic and adult urothelial progenitors. Without Eed, the obligatory subunit of PRC2, embryonic urothelial progenitors demonstrate reduced proliferation with concomitant dysregulation of genes including Cdkn2a (p16), Cdkn2b (p15) and Shh. These mutants display premature differentiation of keratin 5-positive (Krt5(+)) basal cells and ectopic expression of squamous-like differentiation markers...
February 1, 2017: Development
https://www.readbyqxmd.com/read/28040322/mucosectomy-impairs-ileal-microcirculation-and-results-in-flap-contraction-after-experimental-ileocystoplasty
#18
Raimondo M Cervellione, Daniel Hajnal, Gabreilla Varga, George Rakoczy, Jozsef Kaszaki, David Keene, Anju Goyal, Alan Dickson, Tamas Cserni
INTRODUCTION: Bladder augmentation with demucosalized ileal flap is a promising alternative approach for mucus free bladder augmentation; however, the contraction of the flap is still a major concern. It has been hypothesized that mucosectomy causes ischemic damage, but no direct histological evidence has been found and attention is now focused on the urothelium cover to prevent the exposure of the denuded surface to urine or the use of balloons to keep the flaps distended. OBJECTIVE: Our aim was to study the effect of mucosectomy on the microcirculation of ileal flaps during reverse clam ileocystoplasty using direct intraoperative imaging of the ileum...
November 29, 2016: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28002455/evidence-for-the-role-of-mast-cells-in-cystitis-associated-lower-urinary-tract-dysfunction-a-multidisciplinary-approach-to-the-study-of-chronic-pelvic-pain-research-network-animal-model-study
#19
Xu Wang, Wujiang Liu, Michael O'Donnell, Susan Lutgendorf, Catherine Bradley, Andrew Schrepf, Liwei Liu, Karl Kreder, Yi Luo
Bladder inflammation frequently causes cystitis pain and lower urinary tract dysfunction (LUTD) such as urinary frequency and urgency. Although mast cells have been identified to play a critical role in bladder inflammation and pain, the role of mast cells in cystitis-associated LUTD has not been demonstrated. Interstitial cystitis/bladder pain syndrome (IC/BPS) is a chronic and debilitating inflammatory condition of the urinary bladder characterized by the hallmark symptoms of pelvic pain and LUTD. In this study we investigated the role of mast cells in LUTD using a transgenic autoimmune cystitis model (URO-OVA) that reproduces many clinical correlates of IC/BPS...
2016: PloS One
https://www.readbyqxmd.com/read/27995855/recurrent-nephrolithiasis-associated-with-keratinizing-desquamative-squamous-metaplasia
#20
Ryan Fitzpatrick, Luke F Reynolds, James D Watterson, Luke T Lavallée, Trevor A Flood
Keratinizing desquamative squamous metaplasia (KDSM) in the renal pelvis is a rare condition with unclear malignant potential. Recent reports suggest it is likely benign and favor endoscopic treatment approaches. Medical record review was completed on two cases at our center to obtain history, physical examination, radiographic findings, and management. A literature review was completed to identify all published cases of KDSM. Both patients at our center suffered recurrent urolithiasis, hypothesized to be secondary to KDSM...
December 2016: Canadian Journal of Urology
keyword
keyword
38035
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"