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https://www.readbyqxmd.com/read/28237380/newborn-dried-blood-spot-polymerase-chain-reaction-to-identify-infants-with-congenital-cytomegalovirus-associated-sensorineural-hearing-loss
#1
Shannon A Ross, Amina Ahmed, April L Palmer, Marian G Michaels, Pablo J Sánchez, Audra Stewart, David I Bernstein, Kristina Feja, Karen B Fowler, Suresh B Boppana
OBJECTIVE: To determine the utility of dried blood spot (DBS) polymerase chain reaction (PCR) in identifying infants with cytomegalovirus (CMV) infection-associated sensorineural hearing loss (SNHL). STUDY DESIGN: Newborns at 7 US hospitals between March 2007 and March 2012 were screened for CMV by saliva rapid culture and/or PCR. Infected infants were monitored for SNHL during the first 4 years of life to determine sensitivity, specificity, and positive and negative likelihood ratios of DBS PCR for identifying CMV-associated SNHL...
February 22, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28236856/could-mucin-16-and-colony-stimulating-factor-2-receptor-beta-possible-graft-versus-host-disease-biomarkers-medical-hypotheses
#2
Milena Monteiro de Souza, Fabiana Martins de Paula, Ricardo Hsieh, Maria Cristina Martins Almeida Macedo, Marcelo Andreetta Corral, Thaís Borguezan Nunes, Fernanda De Paula, Silvia Vanessa Lourenço
Graft versus host disease (GVHD) occurs after bone marrow transplantation and is one of the most important causes of death worldwide. Reviews demonstrated GVHD patients with involvement of various tissues and organs, such as salivary glands. The diagnosis of acute GVHD has been the biopsies and the histopathologic evaluation of tissue from an involved organ. These procedures are invasive. Saliva proteins as possible biomarker for GVHD could facilitate the management and diagnosis accuracy. For support the proposed hypotheses, in this pilot study we collected whole saliva samples from patients with undergoing allogeneic hematopoietic cell transplantation (HCT) and from healthy subjects...
March 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28235782/development-of-a-multiplexed-lc-mrm-ms-method-for-evaluation-of-salivary-proteins-as-oral-cancer-biomarkers
#3
Yi-Ting Chen, Hsiao-Wei Chen, Chun-Feng Wu, Lichieh Julie Chu, Wei-Fang Chiang, Chih-Ching Wu, Jau-Song Yu, Cheng-Han Tsai, Kung-Hao Liang, Yu-Sun Chang, Maureen Wu, Wei-Ting Ou Yang
Multiple (selected) reaction monitoring (MRM/SRM) of peptides is a growing technology for target protein quantification because it is more robust, precise, accurate, high-throughput, and multiplex-capable than antibody-based techniques. The technique has been applied clinically to the large-scale quantification of multiple target proteins in different types of fluids. However, previous MRM-based studies have placed less focus on sample-preparation workflow and analytical performance in the precise quantification of proteins in saliva, a non-invasively sampled body fluid...
February 24, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28235601/a-part-of-patients-with-autism-spectrum-disorder-has-haploidy-of-hpc-1-syntaxin1a-gene-that-possibly-causes-behavioral-disturbance-as-in-experimentally-gene-ablated-mice
#4
Takefumi Kofuji, Yuko Hayashi, Tomonori Fujiwara, Masumi Sanada, Masao Tamaru, Kimio Akagawa
Autism spectrum disorder (ASD) is highly heritable and encompasses a various set of neuropsychiatric disorders with a wide-ranging presentation. HPC-1/syntaxin1A (STX1A) encodes a neuronal plasma membrane protein that regulates the secretion of neurotransmitters and neuromodulators. STX1A gene ablated mice (null and heterozygote mutant) exhibit abnormal behavioral profiles similar to human autistic symptoms, accompanied by reduction of monoamine secretion. To determine whether copy number variation of STX1A gene and the change of its expression correlate with ASD as in STX1A gene ablated mice, we performed copy number assay and real-time quantitative RT-PCR using blood or saliva samples from ASD patients...
February 21, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28230530/developing-a-multi-component-immune-model-for-evaluating-the-risk-of-respiratory-illness-in-athletes
#5
Maree Gleeson, David B Pyne, Lisa J Elkington, Sharron T Hall, John R Attia, Christopher Oldmeadow, Lisa G Wood, Robin Callister
Clinical and laboratory identification of the underlying risk of respiratory illness in athletes has proved problematic. The aim of this study was to determine whether clinical data, combined with immune responses to standardised exercise protocols and genetic cytokine polymorphism status, could identify the risk of respiratory illness (symptoms) in a cohort of highly-trained athletes. Male endurance athletes (n=16; VO2max 66.5 ± 5.1 mL.kg-1.min-1) underwent a clinical evaluation of known risk factors by a physician and comprehensive laboratory analysis of immune responses both at rest and after two cycling ergometer tests: 60 min at 65% VO2max (LONG); and 6 x 3 min intervals at 90% VO2max (INTENSE)...
2017: Exercise Immunology Review
https://www.readbyqxmd.com/read/28229405/compact-bone-derived-multipotent-mesenchymal-stromal-cells-mscs-for-the-treatment-of-sjogren-s-like-disease-in-nod-mice
#6
Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, Simon D Tran
Compact bone (cortical or dense bone) is among the organs that contain multipotent mesenchymal stromal cells (MSCs). Unlike bone marrow plugs where MSCs were initially isolated, compact bone has minimal (amount of) hematopoietic cells and thus facilitates the MSCs isolation process. In vitro, MSCs from compact bone show multipotency and differentiation into mesenchymal tissues such as bone, adipose, and cartilage, under certain conditions. MSCs therapy has been promising in preclinical and clinical studies against autoimmune diseases...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28227928/pca-criterion-for-svm-mlp-classifier-for-flavivirus-biomarker-from-salivary-sers-spectra-at-febrile-stage
#7
A R M Radzol, Khuan Y Lee, W Mansor, I S Omar, A R M Radzol, Khuan Y Lee, W Mansor, I S Omar, Khuan Y Lee, A R M Radzol, I S Omar, W Mansor
Non-structural protein (NS1) has been conceded as one of the biomarkers for flavivirus that causes diseases with life threatening consequences. NS1 is an antigen that allows detection of the illness at febrile stage, mostly from blood samples currently. Our work here intends to define an optimum model for PCA-SVM with MLP kernel for classification of flavivirus biomarker, NS1 molecule, from SERS spectra of saliva, which to the best of our knowledge has never been explored. Since performance of the model depends on the PCA criterion and MLP parameters, both are examined in tandem...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225785/betel-nut-chewing-oral-premalignant-lesions-and-the-oral-microbiome
#8
Brenda Y Hernandez, Xuemei Zhu, Marc T Goodman, Robert Gatewood, Paul Mendiola, Katrina Quinata, Yvette C Paulino
Oral cancers are attributed to a number of causal agents including tobacco, alcohol, human papillomavirus (HPV), and areca (betel) nut. Although betel nut chewing has been established as an independent cause of oral cancer, the mechanisms of carcinogenesis are poorly understood. An investigation was undertaken to evaluate the influence of betel nut chewing on the oral microbiome and oral premalignant lesions. Study participants were recruited from a dental clinic in Guam. Structured interviews and oral examinations were performed...
2017: PloS One
https://www.readbyqxmd.com/read/28225606/effect-of-astringent-stimuli-on-salivary-protein-interactions-elucidated-by-complementary-proteomics-approaches
#9
Judith Delius, Guillaume Médard, Bernhard Kuster, Thomas Hofmann
The interaction of astringent substances with salivary proteins, which results in protein precipitation, is considered a key event in the molecular mechanism underlying the oral sensation of puckering astringency. As the chemical nature of orally active astringents is diverse and the knowledge on their interactions with salivary proteins rather fragmentary, human whole saliva samples were incubated with supra-threshold and iso-intensity solutions of the astringent polyphenol (-)-epigallocatechin gallate, the multivalent metal salt iron(III) sulfate, the amino-functionalized polysaccharide chitosan, and the basic protein lysozyme...
February 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28223859/histatin-peptides-pharmacological-functions-and-their-applications-in-dentistry
#10
REVIEW
Zohaib Khurshid, Shariq Najeeb, Maria Mali, Syed Faraz Moin, Syed Qasim Raza, Sana Zohaib, Farshid Sefat, Muhammad Sohail Zafar
There are many human oral antimicrobial peptides responsible for playing important roles including maintenance, repairing of oral tissues (hard or soft) and defense against oral microbes. In this review we have highlighted the biochemistry, physiology and proteomics of human oral histatin peptides, secreted from parotid and submandibular salivary glands in human. The significance of these peptides includes capability for ionic binding that can kill fungal Candida albicans. They have histidine rich amino acid sequences (7-12 family members; corresponding to residues 12-24, 13-24, 12-25, 13-25, 5-11, and 5-12, respectively) for Histatin-3...
January 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28223830/two-snps-in-the-promoter-region-of-toll-like-receptor-4-gene-are-not-associated-with-smoking-in-saudi-arabia
#11
Muhammad Kohailan, Mohammad Alanazi, Mahmoud Rouabhia, Abdullah Al Amri, Narasimha Reddy Parine, Abdelhabib Semlali
Defects in the innate immune system, particularly in Toll-like receptors (TLRs), have been reported in several cigarette smoke-promoted diseases. The aim of this study was to examine the impact of tobacco smoke on allelic frequencies of TLR4 single-nucleotide polymorphisms (SNPs) and to compare the genotypic distribution of these SNPs in a Saudi Arabian population with that in previously studied populations. DNA was extracted from 303 saliva samples collected from smokers and nonsmokers. Two transitional SNPs in the promoter region of TLR4 were selected, rs2770150 (T/C) and rs10759931 (G/A)...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28222886/effect-of-different-acidic-solutions-on-the-optical-behavior-of-lithium-disilicate-ceramics
#12
Daniela Micheline Dos Santos, Emily Vivianne Freitas da Silva, Aljomar José Vechiato-Filho, Denis Watanabe, Sandro Basso Bitencourt, Aimée Maria Guiotti, Marcelo Coelho Goiato
STATEMENT OF PROBLEM: The stability of the optical characteristics of dental ceramics is essential. Degradation of these materials resulting from pH or temperature alterations in the oral cavity can lead to treatment failure. PURPOSE: The purpose of this in vitro study was to evaluate the color change (ΔE), the L* coordinate, the translucency parameter, and the contrast ratio of lithium disilicate ceramic exposed to commonly used and potentially colorant solutions...
February 17, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28220901/distinct-signatures-of-dental-plaque-metabolic-byproducts-dictated-by-periodontal-inflammatory-status
#13
Akito Sakanaka, Masae Kuboniwa, Ei Hashino, Takeshi Bamba, Eiichiro Fukusaki, Atsuo Amano
Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings regarding its etiology obtained using high-throughput sequencing technique suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Whole saliva samples were obtained from adult subjects before and after removal of supragingival plaque (debridement)...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219814/biopsychosocial-pathways-linking-subjective-socioeconomic-disadvantage-to-glycemic-control-in-youths-with-type-i-diabetes
#14
Samuele Zilioli, Deborah A Ellis, Justin M Carré, Richard B Slatcher
Older adolescent and young adults (OAYA) with type 1 diabetes (T1D) living in contexts of socio-economic disadvantage (SED) suffer disproportionately from poor glycemic control and related health complications. Although SED may convey a variety of risks, it may exacerbate diabetes-related stress levels, which in turn may account for observed disparities in health outcomes. The primary goal of the present study was to investigate the relationship between subjective SED, diabetes-related perceived stress, and diurnal cortisol secretion in urban OAYA with T1D...
February 3, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28219319/biomarkers-in-alzheimer-s-disease-recent-update
#15
Sushil Sharma, Walter Lipincott
Alzheimer disease (AD) is an age-related neurodegenerative disorder, characterized by loss of memory and cognitive function. It is the common cause of dementia in elderly and is a global health concern as the population of people aged 85 and older, is growing alarmingly. Although pharmacotherapy for the treatment of AD has improved, lot of work remains to treat this devastating disease. AD pathology begins even before the onset of clinical symptoms. Because therapies could be more effective if implemented early in the disease progression, it is highly prudent to discover reliable biomarkers, to detect its exact pathophysiology during pre-symptomatic stage...
February 20, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28218483/antimicrobial-potential-and-gustatory-perception-of-chlorhexidine-gluconate-mouthwashes-with-or-without-alcohol-after-a-single-rinse-a-randomized-controlled-crossover-clinical-trial
#16
R Cantarelli, T C Negrini, Fwmg Muniz, Hjr Oballe, R A Arthur, C K Rösing
OBJECTIVE: The aim of this double-blind randomized clinical trial was to assess whether the presence of alcohol in chlorhexidine gluconate mouthwashes influences their antimicrobial potential against salivary bacteria in young adults. Additionally, the taste perception was assessed. MATERIALS AND METHODS: In a randomized crossover design, 20 participants (17 women and three men; aged 18-38 years old) rinsed with the 0.12% chlorhexidine gluconate with (CHX+) or without alcohol (CHX-) for 1 min...
November 7, 2016: International Journal of Dental Hygiene
https://www.readbyqxmd.com/read/28217778/a-critical-insight-into-the-development-pipeline-of-microfluidic-immunoassay-devices-for-the-sensitive-quantitation-of-protein-biomarkers-at-the-point-of-care
#17
REVIEW
Ana I Barbosa, Nuno M Reis
The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories...
February 20, 2017: Analyst
https://www.readbyqxmd.com/read/28216843/in-vitro-evaluation-of-cytotoxicity-and-corrosion-behavior-of-commercially-pure-titanium-and-ti-6al-4v-alloy-for-dental-implants
#18
Sanchitha Chandar, Ravindra Kotian, Prashanthi Madhyastha, Shama Prasada Kabekkodu, Padmalatha Rao
PURPOSE: The aim of this study was to investigate the cytotoxicity in human gingival fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and corrosion behavior by potentiodynamic polarization technique of commercially pure titanium (Ti 12) and its alloy Ti-6Al-4V (Ti 31). MATERIALS AND METHODS: In the present in vitro study, cytotoxicity of Ti 12 and Ti 31 in human gingival fibroblast by MTT assay and the corrosion behavior by potentiodynamic polarization technique in aqueous solutions of 0...
January 2017: Journal of Indian Prosthodontic Society
https://www.readbyqxmd.com/read/28215894/effect-of-dietary-roughage-level-on-chewing-activity-ruminal-ph-and-saliva-secretion-in-lactating-holstein-cows
#19
F G Jiang, X Y Lin, Z G Yan, Z Y Hu, G M Liu, Y D Sun, X W Liu, Z H Wang
Increasing dietary roughage level is a commonly used strategy to prevent subacute ruminal acidosis. We hypothesized that high-roughage diets could promote chewing activity, saliva secretion, and hence more alkaline to buffer rumen pH. To verify the hypothesis, 12 multiparous Holstein cows in mid-lactation were randomly allocated to 4 treatments in a triplicated 4 × 4 Latin square experiment with one cow in each treatment surgically fitted with a ruminal cannula. Treatments were diets containing 40, 50, 60, or 70% of roughage on a DM basis...
February 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28215400/somatic-mutations-in-tsc1-and-tsc2-cause-focal-cortical-dysplasia
#20
Jae Seok Lim, Ramu Gopalappa, Se Hoon Kim, Suresh Ramakrishna, Minji Lee, Woo-Il Kim, Junho Kim, Sang Min Park, Junehawk Lee, Jung-Hwa Oh, Heung Dong Kim, Chang-Hwan Park, Joon Soo Lee, Sangwoo Kim, Dong Seok Kim, Jung Min Han, Hoon-Chul Kang, Hyongbum Henry Kim, Jeong Ho Lee
Focal cortical dysplasia (FCD) is a major cause of the sporadic form of intractable focal epilepsies that require surgical treatment. It has recently been reported that brain somatic mutations in MTOR account for 15%-25% of FCD type II (FCDII), characterized by cortical dyslamination and dysmorphic neurons. However, the genetic etiologies of FCDII-affected individuals who lack the MTOR mutation remain unclear. Here, we performed deep hybrid capture and amplicon sequencing (read depth of 100×-20,012×) of five important mTOR pathway genes-PIK3CA, PIK3R2, AKT3, TSC1, and TSC2-by using paired brain and saliva samples from 40 FCDII individuals negative for MTOR mutations...
February 12, 2017: American Journal of Human Genetics
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