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Red blood cell blood group antigen

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https://www.readbyqxmd.com/read/28052334/association-of-hla-drb1-and-hla-dqb1-with-red-blood-cell-alloimmunization-in-the-czech-population
#1
A Maluskova, F Mrazek, M Pauliskova, P Kovarova, M Koristka, P Jindra, Z Cermakova
BACKGROUND AND OBJECTIVES: Alloimmune antibodies against red-blood-cell (RBC) antigens induced in susceptible individuals (responders) by transfusion, pregnancy or transplantation may have serious clinical consequences. The aim of this study was to investigate association of alloimmunization against selected RBC antigens with HLA-Class II. MATERIALS AND METHODS: A total of 230 responders (106 monoresponders and 124 multiresponders) were enrolled into the study. HLA-DRB1 and HLA-DQB1 variants were determined by PCR-SSO and their frequencies compared between the patients (patient subgroups) and 375 ethnically and regionally matched controls...
January 4, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28046078/immune-response-in-calves-vaccinated-with-type-three-secretion-system-antigens-and-shiga-toxin-2b-subunit-of-escherichia-coli-o157-h7
#2
Luisina Martorelli, Sergio Garbaccio, Daniel A Vilte, Adriana A Albanese, María P Mejías, Marina S Palermo, Elsa C Mercado, Cristina E Ibarra, Angel A Cataldi
Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group...
2017: PloS One
https://www.readbyqxmd.com/read/28040400/emerging-strategies-of-blood-group-genotyping-for-patients-with-hemoglobinopathies
#3
REVIEW
A Belsito, K Magnussen, C Napoli
Red cell alloimmunization is a serious problem in chronically transfused patients. A number of high-throughput DNA assays have been developed to extend or replace traditional serologic antigen typing. DNA-based typing methods may be easily automated and multiplexed, and provide reliable information on a patient. Molecular genotyping promises to become cheaper, being not dependent on serologic immunoglobulin reagents. Patients with hemoglobinopathies could benefit from receiving extended genomic typing. This could limit post transfusional complications depending on subtle antigenic differences between donors and patients...
December 9, 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28017498/red-cell-immunohematology-research-conducted-in-china
#4
REVIEW
Ziyan Zhu, Luyi Ye, Qin Li, Hongwei Gao, Yinxia Tan, Wei Cai
ABO subtypes and RhD variants are the most studied blood groups in China. Some of the polymorphisms in these two blood groups have direct clinical relevance. Molecular diagnosis of blood group polymorphisms is underway in China. In addition, research groups have developed methods such as screening for blood group mimetic peptides using phage display technology. New reagents, akin to antibodies directed against RhD and ABO, are being investigated using aptamer-based techniques. Progress is also being made in the development of synthetic exoglycosidases for conversion of group A and/or B antigens to group O...
November 24, 2016: Transfusion Medicine Reviews
https://www.readbyqxmd.com/read/27991657/molecular-typing-for-blood-group-antigens-within-40%C3%A2-min-by-direct-polymerase-chain-reaction-from-plasma-or-serum
#5
Franz F Wagner, Willy A Flegel, Rita Bittner, Andrea Döscher
Determining blood group antigens by serological methods may be unreliable in certain situations, such as in patients after chronic or massive transfusion. Red cell genotyping offers a complementary approach, but current methods may take much longer than conventional serological typing, limiting their utility in urgent situations. To narrow this gap, we devised a rapid method using direct polymerase chain reaction (PCR) amplification while avoiding the DNA extraction step. DNA was amplified by PCR directly from plasma or serum of blood donors followed by a melting curve analysis in a capillary rapid-cycle PCR assay...
December 19, 2016: British Journal of Haematology
https://www.readbyqxmd.com/read/27984616/-study-of-the-molecular-basis-of-an-individual-with-aw43-subtype-of-the-abo-blood-group
#6
Danfei Deng, Genjie Lu, Xiaozhe Hong, Xianguo Xu, Ji He, Faming Zhu
OBJECTIVE: To explore the molecular basis of an individual with A subtype of the ABO blood group. METHODS: The ABO antigen and serum antibody of the proband and his parents and sister were detected by a serological method. The whole coding regions of the ABO gene were amplified by PCR and subjected to bidirectional sequencing. RESULTS: Red blood cells of the proband showed mixed field agglutination with anti-A but did not agglutinate with anti-B, and his serum did not agglutinate with A and O cells but with B cells...
December 10, 2016: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/27976800/clinical-value-of-jointly-detection-serum-lactate-dehydrogenase-pleural-fluid-adenosine-deaminase-and-pleural-fluid-carcinoembryonic-antigen-in-the-identification-of-malignant-pleural-effusion
#7
Fan Zhang, Lijuan Hu, Junjun Wang, Jian Chen, Jie Chen, Yumin Wang
BACKGROUND: Limited data are available for the diagnostic value, and for the diagnostic sensitivity and specificity of joint detection of serum lactate dehydrogenase (sLDH)/pleural fluid adenosine deaminase (pADA) and pleural fluid carcinoembryonic antigen (pCEA) in malignant pleural effusion (MPE). METHODS: We collected 987 pleural effusion specimens (of which 318 were malignant pleural effusion, 374 were tubercular pleural effusion, and 295 were parapneumonic effusion specimens) from the First Affiliated Hospital of Wenzhou Medical University from July 2012 to March 2016...
December 15, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27913515/cellular-immune-responses-in-red-blood-cell-alloimmunization
#8
James C Zimring, Krystalyn E Hudson
In excess of 340 blood group antigens have now been described that vary between individuals. Thus, any unit of blood that is nonautologous represents a significant dose of alloantigen. Most blood group antigens are proteins, which differ by a single amino acid between donors and recipients. Approximately 1 out of every 70 individuals are transfused each year (in the United States alone), which leads to antibody responses to red blood cell (RBC) alloantigens in some transfusion recipients. When alloantibodies are formed, in many cases, RBCs expressing the antigen in question can no longer be safely transfused...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27913514/understanding-red-blood-cell-alloimmunization-triggers
#9
Jeanne E Hendrickson, Christopher A Tormey
Blood group alloimmunization is "triggered" when a person lacking a particular antigen is exposed to this antigen during transfusion or pregnancy. Although exposure to an antigen is necessary for alloimmunization to occur, it is not alone sufficient. Blood group antigens are diverse in structure, function, and immunogenicity. In addition to red blood cells (RBCs), a recipient of an RBC transfusion is exposed to donor plasma, white blood cells, and platelets; the potential contribution of these elements to RBC alloimmunization remains unclear...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27893356/single-stranded-dna-aptamer-targeting-and-neutralization-of-anti-d-alloantibody-a-potential-therapeutic-strategy-for-haemolytic-diseases-caused-by-rhesus-alloantibody
#10
Yinze Zhang, Fan Wu, Manni Wang, Naibao Zhuang, Huayou Zhou, Hua Xu
BACKGROUND: Rhesus (Rh) D antigen is the most important antigen in the Rh blood group system because of its strong immunogenicity. When RhD-negative individuals are exposed to RhD-positive blood, they may produce anti-D alloantibody, potentially resulting in delayed haemolytic transfusion reactions and Rh haemolytic disease of the foetus and newborn, which are difficult to treat. Inhibition of the binding of anti-D antibody with RhD antigens on the surface of red blood cells may effectively prevent immune haemolytic diseases...
November 11, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27893355/performance-evaluation-study-of-id-core-xt-a-high-throughput-blood-group-genotyping-platform
#11
Mónica López, Izaskun Apraiz, Montserrat Rubia, Mercedes Piedrabuena, Maria Azkarate, Barbera Veldhuisen, Miguel Á Vesga, Ellen Van Der Schoot, Fernando Puente, Diego Tejedor
BACKGROUND: Traditionally, red blood cell antigens have been identified using serological methods, but recent advances in molecular biology have made the implementation of methods for genetic testing of most blood group antigens possible. The goal of this study was to validate the performance of the ID CORE XT blood group typing assay. MATERIALS AND METHODS: One thousand independent samples from donors, patients and neonates were collected from three research institutes in Spain and the Netherlands...
November 25, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27891625/validation-of-the-multiplex-ligation-dependent-probe-amplification-assay-and-its-application-on-the-distribution-study-of-the-major-alleles-of-17-blood-group-systems-in-chinese-donors-from-guangzhou
#12
Yanli Ji, Jizhi Wen, Barbera Veldhuisen, Lonneke Haer-Wigman, Zhen Wang, Martin Lodén-van Straaten, Ling Wei, Guangping Luo, Yongshui Fu, C Ellen van der Schoot
BACKGROUND: Genotyping platforms for common red blood cell (RBC) antigens have been successfully applied in Caucasian and black populations but not in Chinese populations. In this study, a genotyping assay based on multiplex ligation-dependent probe amplification (MLPA) technology was applied in a Chinese population to validate the MLPA probes. Subsequently, the comprehensive distribution of 17 blood group systems also was obtained. STUDY DESIGN AND METHODS: DNA samples from 200 Chinese donors were extracted and genotyped using the blood-MLPA assay...
November 27, 2016: Transfusion
https://www.readbyqxmd.com/read/27871240/abo-antigen-and-secretor-statuses-are-not-associated-with-gut-microbiota-composition-in-1-500-twins
#13
Emily R Davenport, Julia K Goodrich, Jordana T Bell, Tim D Spector, Ruth E Ley, Andrew G Clark
BACKGROUND: Host genetics is one of several factors known to shape human gut microbiome composition, however, the physiological processes underlying the heritability are largely unknown. Inter-individual differences in host factors secreted into the gut lumen may lead to variation in microbiome composition. One such factor is the ABO antigen. This molecule is not only expressed on the surface of red blood cells, but is also secreted from mucosal surfaces in individuals containing an intact FUT2 gene (secretors)...
November 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27864153/immunogenicity-of-murine-mpeg-red-blood-cells-and-the-risk-of-anti-peg-antibodies-in-human-blood-donors
#14
Yevgeniya Le, Wendy M Toyofuku, Mark D Scott
The immunocamouflage of non-ABO blood group antigens by membrane-grafted methoxypoly(ethylene glycol) (mPEG) may attenuate the risk of red blood cell (RBC) alloimmunization. However, concerns have been raised over the immunogenic risk of PEG and PEG-RBCs. To assess this risk, murine and human studies were performed. Mice were exposed to soluble PEG prior to, or between, multiple transfusions (∼60-day intervals) of control or mPEG-RBCs, and cell survival was determined by flow cytometry. In some studies, the control and mPEG-RBC groups were reversed after one or more transfusions...
November 15, 2016: Experimental Hematology
https://www.readbyqxmd.com/read/27812254/evaluation-of-magnetized-erythrocyte-group-antigens-to-detect-abo-antibodies
#15
O Nathalang, K Intharanut, P Sriwanitchrak, M Setthakarn, S Duan, H Wang, S Ding, Y Li
Screening for IgM titers of anti-A and anti-B is recommended when providing ABO incompatible platelet transfusion. The life-time of reagent cells depends upon the preservative diluents. We aimed to evaluate the IgM titers of anti-A and anti-B testing with magnetized-erythrocyte group antigens (MEGA) and fresh RBCs and study the relationship of ABO antibody titers between both techniques. Altogether, 100 serum samples from group O donors at the National Blood Centre, Thai Red Cross Society, Bangkok, Thailand were included...
December 2016: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/27809976/feeding-horses-with-industrially-manufactured-pellets-with-fungal-spores-to-promote-nematode-integrated-control
#16
José Ángel Hernández, Fabián Leonardo Arroyo, José Suárez, Cristiana Filipa Cazapal-Monteiro, Ángel Romasanta, María Eugenia López-Arellano, José Pedreira, Luis Manuel Madeira de Carvalho, Rita Sánchez-Andrade, María Sol Arias, Pedro Mendoza de Gives, Adolfo Paz-Silva
The usefulness of pellets industrially manufactured with spores of parasiticide fungi as a contribution to integrated nematode control was assessed in grazing horses throughout sixteen months. Two groups of 7 Pura Raza Galega autochthonous horses (G-T and G-P) were dewormed pour-on (1mg Ivermectin/kg bw) at the beginning of the trial, and other group (G-C) remained untreated. The G-P was provided daily with commercial pellets to which was added a mixture of fungal spores during the industrial manufacturing (2×10(6) spores of Mucor circinelloides and same dose of Duddingtonia flagrans/kg), and G-T and G-C received pellets without spores...
October 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27807866/a-jordanian-family-with-three-sisters-apparently-homozygous-for-m-k-and-evidence-for-clinical-significance-of-antibodies-produced-by-m-k-m-k-individuals
#17
Nour Al-Huda Al-Jada
BACKGROUND: The rare M(k) M(k) phenotype is the result of a deletion of the coding regions of both GYPA and GYPB. Red blood cells (RBCs) of individuals homozygous for the rare M(k) gene lack all MNS blood group antigens and have no glycophorin A or glycophorin B. This phenotype is extremely rare and only four families have been reported. CASE REPORT: A 28-year-old woman was referred for assessment of recurrent early neonatal deaths. She was found to be apparently homozygous for M(k) ...
November 3, 2016: Transfusion
https://www.readbyqxmd.com/read/27794527/the-challenges-of-daratumumab-in-transfusion-medicine
#18
REVIEW
Michael F Dizon
The field of transfusion medicine has evolved rapidly in recent years, but the central principle of transfusion is still simple, namely, the antigen-antibody interaction. Daratumumab (DARA), a monoclonal antibody (MoAb), was developed to treat relapsed/refractory multiple myeloma (RRMM). DARA works by targeting the CD38 portion of malignant cells; however, this drug attaches to the red blood cell (RBC) reagents used in blood banks, further complicating the antibody identification work-up. The AABB (formerly known as the American Association of Blood Banks) has issued a memorandum on how blood banks can effectively address panreactivity caused by DARA...
October 28, 2016: Laboratory Medicine
https://www.readbyqxmd.com/read/27784393/-indentification-and-sequence-analysis-of-a-individual-with-b305-blood-group-gene-subtypes-of-abo-variant
#19
Gui-Ji Li, Xu Zhang
OBJECTIVE: To investigate and identify the molecular and biological characteristics of B305 blood group gene subtypes of a ABO variant. METHODS: The individual was confirmed by standard serological techniques. The genotyping and sequencing were performed by using polymerase chain reaction-sequence specific primer (PCR-SSP), direct sequencing and gene cloning for Exon 6 and exon 7 of ABO locus respectively. RESULTS: B antigen was detected on red blood cells of the proband and there was anti-A antibody in the serum...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27717418/advances-in-blood-typing
#20
N Quraishy, S Sapatnekar
The clinical importance of blood group antigens relates to their ability to evoke immune antibodies that are capable of causing hemolysis. The most important antigens for safe transfusion are ABO and D (Rh), and typing for these antigens is routinely performed for patients awaiting transfusion, prenatal patients, and blood donors. Typing for other blood group antigens, typically of the Kell, Duffy, Kidd, and MNS blood groups, is sometimes necessary, for patients who have, or are likely to develop antibodies to these antigens...
2016: Advances in Clinical Chemistry
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