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Red blood cell blood group antigen

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https://www.readbyqxmd.com/read/28191636/cancer-type-predicts-alloimmunization-following-rhd-incompatible-rbc-transfusions
#1
Komal Arora, James Kelley, Dawen Sui, Jing Ning, Fernando Martinez, Benjamin Lichtiger, Ashok Tholpady
BACKGROUND: Immunosuppressed, RhD-negative oncology patients tend to have lower rates of sensitization to the D antigen when they receive transfusion with RhD-positive blood components. Clinical factors associated with alloimmunization to the D antigen in RhD-negative oncology patients when they receive transfusion with RhD-positive red blood cells (RBCs) have not been well defined. STUDY DESIGN AND METHODS: This was a 4-year, retrospective analysis identifying RhD-negative oncology patients who received RhD-positive RBCs and were not previously alloimmunized to the D antigen...
February 12, 2017: Transfusion
https://www.readbyqxmd.com/read/28164304/blood-group-antigen-matching-influence-on-gestational-outcomes-amigo-study
#2
Meghan Delaney, Agneta Wikman, Leo van de Watering, Henk Schonewille, Jennie P Verdoes, Stephen P Emery, Michael F Murphy, Julie Staves, Susanne Flach, Donald M Arnold, Richard M Kaufman, Alyssa Ziman, Sarah K Harm, Mark Fung, Catherine S Eppes, Nancy M Dunbar, Andreas Buser, Erin Meyer, Helen Savoia, Padmakumari Abeysinghe, Nancy Heddle, Alan Tinmouth, Aicha N Traore, Mark H Yazer
BACKGROUND: Red blood cell (RBC) antigen matching policies to prevent alloimmunization in females of childbearing potential (FCP) vary between centers. To inform transfusion centers responsible for making decisions about matching policies for FCPs, the causal stimulus of the antibodies implicated in severe hemolytic disease of the fetus and newborn (HDFN) must be determined. STUDY DESIGN AND METHODS: We conducted a multinational retrospective study of women with offspring affected by severe HDFN requiring neonatal exchange transfusion and/or intrauterine transfusion...
February 5, 2017: Transfusion
https://www.readbyqxmd.com/read/28157207/mapping-the-distribution-of-specific-antibody-interaction-forces-on-individual-red-blood-cells
#3
Natasha Yeow, Rico F Tabor, Gil Garnier
Current blood typing methods rely on the agglutination of red blood cells (RBCs) to macroscopically indicate a positive result. An indirect agglutination mechanism is required when blood typing with IgG forms of antibodies. To date, the interaction forces between anti-IgG and IgG antibodies have been poorly quantified, and blood group related antigens have never been quantified with the atomic force microscope (AFM). Instead, the total intensity resulting from fluorescent-tagged antibodies adsorbed on RBC has been measured to calculate an average antigen density on a series of RBCs...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28151395/molecular-basis-of-the-duffy-blood-group-system
#4
REVIEW
Gabriela Höher, Marilu Fiegenbaum, Silvana Almeida
ACKR1, located on chromosome 1q23.2, is the gene that encodes a glycoprotein expressing the Duffy blood group antigens. This gene is transcribed in two mRNA variants yielding two isoforms, encoding proteins with 338 and 336 amino acids. This review provides a general overview of the Duffy blood group to characterise and elucidate the genetic basis of this system. The Fy(a) and Fy(b) antigens are encoded by co-dominant FY*A (FY*01) and FY*B (FY*02) alleles, which differ by c.125G>A (rs12075), defining the Fy(a+b-), Fy(a-b+) and Fy(a+b+) phenotypes...
January 30, 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28127141/blood-group-change-in-acute-myeloid-leukemia
#5
Rakul K Nambiar, Geetha Narayanan, N P Prakash, K Vijayalakshmi
Blood group antigens are either sugars or proteins found attached to the red blood cell membrane. ABO blood group antigens are the most clinically important antigens because they are the most immunogenic. As red blood cell antigens are inherited traits, they are usually not altered throughout the life of an individual. There have been occasional case reports of ABO blood group antigen change in malignant conditions. We report two such cases of ABO antigen alteration associated with acute myeloid leukemia. These patients had suppression of their blood group antigens during their leukemic phase, and the antigens were reexpressed when the patients attained remission...
January 2017: Proceedings of the Baylor University Medical Center
https://www.readbyqxmd.com/read/28112245/abo-typing-in-experimental-cynomolgus-monkeys-using-non-invasive-methods
#6
Xiaoxiao Wang, Song Chen, Gang Chen
ABH antigens are not expressed on the red blood cells of monkeys, making it difficult to accurately determine their blood type. In this study, we evaluated the feasibility, convenience, and stability of two non-invasive methods for ABO typing (a reverse gel system assay and a buccal mucosal cell immunofluorescent assay) in cynomolgus monkeys (n = 72). The renal tissue immunofluorescent assay was used to obtain an accurate blood type in the monkeys. Using the reverse gel system assay and preabsorbed serum, we achieved accurate detection of ABO blood groups in 65 of the 72 monkeys but obtained confusing results in the remaining 7...
January 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28111825/effect-of-red-blood-cell-preservation-by-droplet-freezing-with-non-permeable-cryoprotective-agents-in-blood-group-antigen-reactivity
#7
M A B Chagas, D G Chaves, S K Haddad, E M A Ubiali, L C Schmidt, M C F Silva-Malta
BACKGROUND: In the last few decades, various red blood cell (RBC) freezing techniques have been developed and improved to enable the preservation of erythrocytes for future use in pre-transfusion tests in reference immunohaematology laboratories. However, not all these techniques have been sufficiently evaluated for the preservation of blood group antigens. OBJECTIVES: In this study, we evaluated the antigenic pattern of RBCs preserved by droplet freezing in liquid nitrogen in a blood bank context...
January 22, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28102039/a-dispermic-chimerism-detected-in-a-taiwanese-potential-unrelated-hematopoietic-stem-cell-donor
#8
E K L Yang, S G E Marsh, P-Y Chen, C-P Chen, S-P Chen, P Y Lin
Chimerism is defined as the presence of 2 or more than 1 genetically distinct cell populations in an organism. Dispermic chimeras are derived from the fertilization of 1 or 2 matured nuclei by 2 sperms. We here report detection of a healthy and phenotypically normal female with normal ABO red blood cell typing in whom dispermic chimerism was suspected after 3 alleles were identified at multiple human leukocyte antigen (HLA) loci using molecular HLA analysis. Molecular HLA typing showed the donor to have 3 HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 alleles in blood, saliva and nail samples...
February 2017: HLA
https://www.readbyqxmd.com/read/28052334/association-of-hla-drb1-and-hla-dqb1-with-red-blood-cell-alloimmunization-in-the-czech-population
#9
A Maluskova, F Mrazek, M Pauliskova, P Kovarova, M Koristka, P Jindra, Z Cermakova
BACKGROUND AND OBJECTIVES: Alloimmune antibodies against red-blood-cell (RBC) antigens induced in susceptible individuals (responders) by transfusion, pregnancy or transplantation may have serious clinical consequences. The aim of this study was to investigate association of alloimmunization against selected RBC antigens with HLA-Class II. MATERIALS AND METHODS: A total of 230 responders (106 monoresponders and 124 multiresponders) were enrolled into the study. HLA-DRB1 and HLA-DQB1 variants were determined by PCR-SSO and their frequencies compared between the patients (patient subgroups) and 375 ethnically and regionally matched controls...
January 4, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28046078/immune-response-in-calves-vaccinated-with-type-three-secretion-system-antigens-and-shiga-toxin-2b-subunit-of-escherichia-coli-o157-h7
#10
Luisina Martorelli, Sergio Garbaccio, Daniel A Vilte, Adriana A Albanese, María P Mejías, Marina S Palermo, Elsa C Mercado, Cristina E Ibarra, Angel A Cataldi
Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group...
2017: PloS One
https://www.readbyqxmd.com/read/28040400/emerging-strategies-of-blood-group-genotyping-for-patients-with-hemoglobinopathies
#11
REVIEW
A Belsito, K Magnussen, C Napoli
Red cell alloimmunization is a serious problem in chronically transfused patients. A number of high-throughput DNA assays have been developed to extend or replace traditional serologic antigen typing. DNA-based typing methods may be easily automated and multiplexed, and provide reliable information on a patient. Molecular genotyping promises to become cheaper, being not dependent on serologic immunoglobulin reagents. Patients with hemoglobinopathies could benefit from receiving extended genomic typing. This could limit post transfusional complications depending on subtle antigenic differences between donors and patients...
December 9, 2016: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28017498/red-cell-immunohematology-research-conducted-in-china
#12
REVIEW
Ziyan Zhu, Luyi Ye, Qin Li, Hongwei Gao, Yinxia Tan, Wei Cai
ABO subtypes and RhD variants are the most studied blood groups in China. Some of the polymorphisms in these two blood groups have direct clinical relevance. Molecular diagnosis of blood group polymorphisms is underway in China. In addition, research groups have developed methods such as screening for blood group mimetic peptides using phage display technology. New reagents, akin to antibodies directed against RhD and ABO, are being investigated using aptamer-based techniques. Progress is also being made in the development of synthetic exoglycosidases for conversion of group A and/or B antigens to group O...
November 24, 2016: Transfusion Medicine Reviews
https://www.readbyqxmd.com/read/27991657/molecular-typing-for-blood-group-antigens-within-40%C3%A2-min-by-direct-polymerase-chain-reaction-from-plasma-or-serum
#13
Franz F Wagner, Willy A Flegel, Rita Bittner, Andrea Döscher
Determining blood group antigens by serological methods may be unreliable in certain situations, such as in patients after chronic or massive transfusion. Red cell genotyping offers a complementary approach, but current methods may take much longer than conventional serological typing, limiting their utility in urgent situations. To narrow this gap, we devised a rapid method using direct polymerase chain reaction (PCR) amplification while avoiding the DNA extraction step. DNA was amplified by PCR directly from plasma or serum of blood donors followed by a melting curve analysis in a capillary rapid-cycle PCR assay...
December 19, 2016: British Journal of Haematology
https://www.readbyqxmd.com/read/27984616/-study-of-the-molecular-basis-of-an-individual-with-aw43-subtype-of-the-abo-blood-group
#14
Danfei Deng, Genjie Lu, Xiaozhe Hong, Xianguo Xu, Ji He, Faming Zhu
OBJECTIVE: To explore the molecular basis of an individual with A subtype of the ABO blood group. METHODS: The ABO antigen and serum antibody of the proband and his parents and sister were detected by a serological method. The whole coding regions of the ABO gene were amplified by PCR and subjected to bidirectional sequencing. RESULTS: Red blood cells of the proband showed mixed field agglutination with anti-A but did not agglutinate with anti-B, and his serum did not agglutinate with A and O cells but with B cells...
December 10, 2016: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/27976800/clinical-value-of-jointly-detection-serum-lactate-dehydrogenase-pleural-fluid-adenosine-deaminase-and-pleural-fluid-carcinoembryonic-antigen-in-the-identification-of-malignant-pleural-effusion
#15
Fan Zhang, Lijuan Hu, Junjun Wang, Jian Chen, Jie Chen, Yumin Wang
BACKGROUND: Limited data are available for the diagnostic value, and for the diagnostic sensitivity and specificity of joint detection of serum lactate dehydrogenase (sLDH)/pleural fluid adenosine deaminase (pADA) and pleural fluid carcinoembryonic antigen (pCEA) in malignant pleural effusion (MPE). METHODS: We collected 987 pleural effusion specimens (of which 318 were malignant pleural effusion, 374 were tubercular pleural effusion, and 295 were parapneumonic effusion specimens) from the First Affiliated Hospital of Wenzhou Medical University from July 2012 to March 2016...
December 15, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27913515/cellular-immune-responses-in-red-blood-cell-alloimmunization
#16
James C Zimring, Krystalyn E Hudson
In excess of 340 blood group antigens have now been described that vary between individuals. Thus, any unit of blood that is nonautologous represents a significant dose of alloantigen. Most blood group antigens are proteins, which differ by a single amino acid between donors and recipients. Approximately 1 out of every 70 individuals are transfused each year (in the United States alone), which leads to antibody responses to red blood cell (RBC) alloantigens in some transfusion recipients. When alloantibodies are formed, in many cases, RBCs expressing the antigen in question can no longer be safely transfused...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27913514/understanding-red-blood-cell-alloimmunization-triggers
#17
Jeanne E Hendrickson, Christopher A Tormey
Blood group alloimmunization is "triggered" when a person lacking a particular antigen is exposed to this antigen during transfusion or pregnancy. Although exposure to an antigen is necessary for alloimmunization to occur, it is not alone sufficient. Blood group antigens are diverse in structure, function, and immunogenicity. In addition to red blood cells (RBCs), a recipient of an RBC transfusion is exposed to donor plasma, white blood cells, and platelets; the potential contribution of these elements to RBC alloimmunization remains unclear...
December 2, 2016: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/27893356/single-stranded-dna-aptamer-targeting-and-neutralization-of-anti-d-alloantibody-a-potential-therapeutic-strategy-for-haemolytic-diseases-caused-by-rhesus-alloantibody
#18
Yinze Zhang, Fan Wu, Manni Wang, Naibao Zhuang, Huayou Zhou, Hua Xu
BACKGROUND: Rhesus (Rh) D antigen is the most important antigen in the Rh blood group system because of its strong immunogenicity. When RhD-negative individuals are exposed to RhD-positive blood, they may produce anti-D alloantibody, potentially resulting in delayed haemolytic transfusion reactions and Rh haemolytic disease of the foetus and newborn, which are difficult to treat. Inhibition of the binding of anti-D antibody with RhD antigens on the surface of red blood cells may effectively prevent immune haemolytic diseases...
November 11, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27893355/performance-evaluation-study-of-id-core-xt-a-high-throughput-blood-group-genotyping-platform
#19
Mónica López, Izaskun Apraiz, Montserrat Rubia, Mercedes Piedrabuena, Maria Azkarate, Barbera Veldhuisen, Miguel Á Vesga, Ellen Van Der Schoot, Fernando Puente, Diego Tejedor
BACKGROUND: Traditionally, red blood cell antigens have been identified using serological methods, but recent advances in molecular biology have made the implementation of methods for genetic testing of most blood group antigens possible. The goal of this study was to validate the performance of the ID CORE XT blood group typing assay. MATERIALS AND METHODS: One thousand independent samples from donors, patients and neonates were collected from three research institutes in Spain and the Netherlands...
November 25, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27891625/validation-of-the-multiplex-ligation-dependent-probe-amplification-assay-and-its-application-on-the-distribution-study-of-the-major-alleles-of-17-blood-group-systems-in-chinese-donors-from-guangzhou
#20
Yanli Ji, Jizhi Wen, Barbera Veldhuisen, Lonneke Haer-Wigman, Zhen Wang, Martin Lodén-van Straaten, Ling Wei, Guangping Luo, Yongshui Fu, C Ellen van der Schoot
BACKGROUND: Genotyping platforms for common red blood cell (RBC) antigens have been successfully applied in Caucasian and black populations but not in Chinese populations. In this study, a genotyping assay based on multiplex ligation-dependent probe amplification (MLPA) technology was applied in a Chinese population to validate the MLPA probes. Subsequently, the comprehensive distribution of 17 blood group systems also was obtained. STUDY DESIGN AND METHODS: DNA samples from 200 Chinese donors were extracted and genotyped using the blood-MLPA assay...
November 27, 2016: Transfusion
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