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https://www.readbyqxmd.com/read/28335308/probe-microscopic-studies-of-dna-molecules-on-carbon-nanotubes
#1
REVIEW
Kazuo Umemura, Katsuki Izumi, Shusuke Oura
Hybrids of DNA and carbon nanotubes (CNTs) are promising nanobioconjugates for nanobiosensors, carriers for drug delivery, and other biological applications. In this review, nanoscopic characterization of DNA-CNT hybrids, in particular, characterization by scanning probe microscopy (SPM), is summarized. In many studies, topographical imaging by atomic force microscopy has been performed. However, some researchers have demonstrated advanced SPM operations in order to maximize its unique and valuable functions...
October 8, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28333363/evaluation-of-the-%C3%AE-carba%C3%A2-test-a-colorimetric-test-for-the-rapid-detection-of-carbapenemase-activity-in-gram-negative-bacilli
#2
Sandrine Bernabeu, Laurent Dortet, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated a novel colorimetric test (the β-CARBA™ test; Bio-Rad) to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. Methods: The performance of the β-CARBA™ test was compared with that of the Carba NP test (or the CarbAcineto NP test) and RAPIDEC ® CARBA NP (bioMérieux) using a collection of 290 isolates with characterized β-lactamase content...
March 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28324129/detection-of-tetrabromobisphenol-a-and-its-mono-and-dimethyl%C3%A2-derivatives-in-fish-sediment-and-suspended-particulate-matter-from-european-freshwaters-and-estuaries
#3
Matthias Kotthoff, Heinz Rüdel, Heinrich Jürling
An analytical method was developed for the determination of tetrabromobisphenol A (TBBPA), 3,3',5,5'-tetrabromobisphenol-A-monomethyl ether (MM-TBBPA) and 3,3',5,5'-tetrabromobisphenol-A-dimethyl ether (DM-TBBPA), and its valid application on fish muscle matrix (bream and sole), suspended particulate matter (SPM) and surface sediment layer samples, using only 0.5 g sample material, is demonstrated. Here, for the first time, DM-TBBPA could be determined by an LC-MS/MS-based method applying atmospheric pressure photoionization (APPI), using the same sample extracts for all three analytes...
March 21, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28322026/objective-3d-surface-evaluation-of-intracranial-electrophysiologic-correlates-of-cerebral-glucose-metabolic-abnormalities-in-children-with-focal-epilepsy
#4
Jeong-Won Jeong, Eishi Asano, Vinod Kumar Pilli, Yasuo Nakai, Harry T Chugani, Csaba Juhász
To determine the spatial relationship between 2-deoxy-2[(18) F]fluoro-D-glucose (FDG) metabolic and intracranial electrophysiological abnormalities in children undergoing two-stage epilepsy surgery, statistical parametric mapping (SPM) was used to correlate hypo- and hypermetabolic cortical regions with ictal and interictal electrocorticography (ECoG) changes mapped onto the brain surface. Preoperative FDG-PET scans of 37 children with intractable epilepsy (31 with non-localizing MRI) were compared with age-matched pseudo-normal pediatric control PET data...
March 21, 2017: Human Brain Mapping
https://www.readbyqxmd.com/read/28315844/cerebral-glucose-metabolism-and-cognition-in-newly-diagnosed-parkinson-s-disease-icicle-pd-study
#5
M J Firbank, A J Yarnall, R A Lawson, G W Duncan, T K Khoo, G S Petrides, J T O'Brien, R A Barker, R J Maxwell, D J Brooks, D J Burn
OBJECTIVE: To assess reductions of cerebral glucose metabolism in Parkinson's disease (PD) with 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET), and their associations with cognitive decline. METHODS: FDG-PET was performed on a cohort of 79 patients with newly diagnosed PD (mean disease duration 8 months) and 20 unrelated controls. PD participants were scanned while on their usual dopaminergic medication. Cognitive testing was performed at baseline, and after 18 months using the Cognitive Drug Research (CDR) and Cambridge Neuropsychological Test Automated Battery (CANTAB) computerised batteries, the Mini-Mental State Examination (MMSE), and the Montreal Cognitive Assessment (MoCA)...
April 2017: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/28315656/expression-of-odc1-spd-spm-and-azin1-in-the-hypothalamus-ovary-and-uterus-during-rat-estrous-cycle
#6
Joseph R D Fernandes, Sammit Jain, Arnab Banerjee
The aim of the present study was to investigate variation in the expression pattern of ornithine decarboxylase (ODC1), spermine (SPM), spermidine (SPD) and antizyme inhibitor (AZIN1) in hypothalamus, ovary and uterus during the estrous cycle of rats. Further, to understand any correlation between polyamines and GnRH I expression in hypothalamus; effect of putrescine treatment on GnRH I expression in hypothalamus and progesterone and estradiol levels in serum were investigated. The study also aims in quantifying all the immunohistochemistry images obtained based on pixel counting algorithm to yield the relative pixel count...
March 15, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28304064/surface-point-defects-on-bulk-oxides-atomically-resolved-scanning-probe-microscopy
#7
Martin Setvín, Margareta Wagner, Michael Schmid, Gareth S Parkinson, Ulrike Diebold
Metal oxides are abundant in nature and they are some of the most versatile materials for applications ranging from catalysis to novel electronics. The physical and chemical properties of metal oxides are dramatically influenced, and can be judiciously tailored, by defects. Small changes in stoichiometry introduce so-called intrinsic defects, e.g., atomic vacancies and/or interstitials. This review gives an overview of using Scanning Probe Microscopy (SPM), in particular Scanning Tunneling Microscopy (STM), to study the changes in the local geometric and electronic structure related to these intrinsic point defects at the surfaces of metal oxides...
March 17, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28283793/diagnosis-and-treatment-of-spontaneous-pneumomediastinum-experience-at-a-single-institution-for-10%C3%A2-years
#8
In-Hag Song, Seock Yeol Lee, Seung Jin Lee, Won Suk Choi
OBJECTIVE: The aim of this study was to identify appropriate method of diagnosis and treatment of spontaneous pneumomediastinum (SPM) based on our experience. METHODS: The medical records of patients who were diagnosed with SPM and treated at our hospital between April 2006 and July 2015 were, retrospectively, analyzed. The data included characteristics of the patients, method of diagnosis, treatment and clinical course. RESULTS: Forty-five patients were diagnosed with SPM and treated at our hospital...
March 10, 2017: General Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28278425/impacts-of-human-activities-on-the-spatial-distribution-and-sources-of-polychlorinated-naphthalenes-in-the-middle-and-lower-reaches-of-the-yellow-river
#9
Qilu Li, Xianghui Cheng, Yan Wang, Zhineng Cheng, Liya Guo, Kechang Li, Xianfa Su, Jianhui Sun, Jun Li, Gan Zhang
The concentrations and compositions of polychlorinated naphthalenes (PCNs) in sediments and suspended particulate matters (SPM) in the middle and lower reaches of the Yellow River were investigated. The mean concentrations of PCNs were 7.15 ± 19.3 ng/g dw in the sediment and 38.1 ± 58.4 ng/g dw in SPM. Tri- and tetra-CNs were the dominant homologue groups in most samples. CN-23 was the predominant congener at all sites, and its presence may be attributed to coal combustion. Combustion indicators showed that local combustion source was the main contributor to the PCN concentrations...
February 27, 2017: Chemosphere
https://www.readbyqxmd.com/read/28275348/cerebromatic-a-versatile-toolbox-for-spline-based-mri-template-creation
#10
Marko Wilke, Mekibib Altaye, Scott K Holland
Brain image spatial normalization and tissue segmentation rely on prior tissue probability maps. Appropriately selecting these tissue maps becomes particularly important when investigating "unusual" populations, such as young children or elderly subjects. When creating such priors, the disadvantage of applying more deformation must be weighed against the benefit of achieving a crisper image. We have previously suggested that statistically modeling demographic variables, instead of simply averaging images, is advantageous...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/28268725/low-power-operation-of-a-barometric-pressure-sensor-for-use-in-an-automatic-fall-detector
#11
Wei Lu, Changhong Wang, Michael C Stevens, Stephen J Redmond, Nigel H Lovell
The use of a barometric pressure sensor in a wearable fall detector has been shown to improve the detection accuracy by determining the altitude change associated with the fall event. However, the barometer is a high-power-consuming sensor. This paper proposes a fall detection approach using a hermetically sealed and waterproof enclosure incorporating a small window covered by a semi-permeable membrane (SPM) to delay the equilibrium of internal and external pressures. This feature can be utilized to limit the time the barometer is powered but still capturing critical pressure information to discriminate fall and non-fall events...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28266834/direct-probing-of-the-dielectric-scavenging-layer-interface-in-oxide-filamentary-based-valence-change-memory
#12
Umberto Celano, Jonathan Op de Beeck, Sergiu Clima, Michael Lübben, Paul M Koenraad, Ludovic Goux, Ilia Valov, Wilfried Vandervorst
A great improvement in valence change memory performance has been recently achieved by adding another metallic layer to the simple metal-insulator-metal (MIM) structure. This metal layer is often referred to as oxygen exchange layer (OEL) and is introduced between one of the electrodes and the oxide. The OEL is believed to induce a distributed reservoir of defects at the metal-insulator interface thus providing an unlimited availability of building blocks for the conductive filament (CF). However, its role remains elusive and controversial owing to the difficulties to probe the interface between the OEL and the CF...
March 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28266002/voxel-based-mapping-of-grey-matter-volume-and-glucose-metabolism-profiles-in-amyotrophic-lateral-sclerosis
#13
M-S Buhour, F Doidy, A Mondou, A Pélerin, L Carluer, F Eustache, F Viader, B Desgranges
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a rapidly progressive disease of the nervous system involving both upper and lower motor neurons. The patterns of structural and metabolic brain alterations are still unclear. Several studies using anatomical MRI yielded a number of discrepancies in their results, and a few PET studies investigated the effect of ALS on cerebral glucose metabolism. The aim of this study was threefold: to highlight the patterns of grey matter (GM) atrophy, hypometabolism and hypermetabolism in patients with ALS, then to understand the neurobehavioral significance of hypermetabolism and, finally, to investigate the regional differences between the morphologic and functional changes in ALS patients, using a specially designed voxel-based method...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28263773/discovery-of-specialized-pro-resolving-mediators-marks-the-dawn-of-resolution-physiology-and-pharmacology
#14
REVIEW
Charles N Serhan
It is with great pleasure that I write this foreword and introduction to this Special Issue dedicated to the protective actions of the pro-resolving mediators and edited by my colleague Dr. Jesmond Dalli. Many of my collaborators and colleagues that helped to uncover the actions and clinical potential of the resolvins and other specialized proresolving mediators (SPM), namely, the superfamily of pro-resolving mediators that includes the resolvin (E-series, D-series and DPA-derived), protectin and maresin families, as well as the arachidonic acid-derived lipoxins, join me in this special issue...
March 3, 2017: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/28261254/interaction-of-polyamines-abscisic-acid-nitric-oxide-and-hydrogen-peroxide-under-chilling-stress-in-tomato-lycopersicon-esculentum-mill-seedlings
#15
Qiannan Diao, Yongjun Song, Dongmei Shi, Hongyan Qi
Polyamines (PAs) play a vital role in the responses of higher plants to abiotic stresses. However, only a limited number of studies have examined the interplay between PAs and signal molecules. The aim of this study was to elucidate the cross-talk among PAs, abscisic acid (ABA), nitric oxide (NO), and hydrogen peroxide (H2O2) under chilling stress conditions using tomato seedlings [(Lycopersicon esculentum Mill.) cv. Moneymaker]. The study showed that during chilling stress (4°C; 0, 12, and 24 h), the application of spermidine (Spd) and spermine (Spm) elevated NO and H2O2 levels, enhanced nitrite reductase (NR), nitric oxide synthase (NOS)-like, and polyamine oxidase activities, and upregulated LeNR relative expression, but did not influence LeNOS1 expression...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28259081/phytochelatin-synthesis-in-response-to-hg-uptake-in-aquatic-plants-near-a-chlor-alkali-factory
#16
Marta Turull, Gabriela Grmanova, Àngela Dago, Cristina Ariño, Sergi Díez, José Manuel Díaz-Cruz, Miquel Esteban
The effects of mercury (Hg) released from a chlor-alkali factory in aquatic plants along the Ebro River basin (NE Spain) were analysed considering the phytochelatins (PCn) and their isoforms content in these plants. These compounds were analyzed using HPLC with amperometric detection, and the macrophytes species Ceratophyllum demersum and Myriopyllum spicatum were collected in two sampling campaigns, autumn and spring, respectively. To correlate the PCn content in macrophytes with the Hg contamination, analysis of total Hg (THg) content in plants and suspended particulate matter, as well as the dissolved-bioavailable fraction of Hg in water measured by the diffusive gradient in thin film (DGT) technique were done...
February 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28252254/-19-f-nmr-reveals-the-role-of-mobile-loops-in-product-and-inhibitor-binding-by-the-s%C3%A3-o-paulo-metallo-%C3%AE-lactamase
#17
Martine I Abboud, Philip Hinchliffe, Jürgen Brem, Robert Macsics, Inga Pfeffer, Anne Makena, Klaus-Daniel Umland, Anna M Rydzik, Guo-Bo Li, James Spencer, Timothy D W Claridge, Christopher J Schofield
Resistance to β-lactam antibiotics mediated by metallo-β-lactamases (MBLs) is a growing problem. We describe the use of protein-observe (19) F-NMR (PrOF NMR) to study the dynamics of the São Paulo MBL (SPM-1) from β-lactam-resistant Pseudomonas aeruginosa. Cysteinyl variants on the α3 and L3 regions, which flank the di-Zn(II) active site, were selectively (19) F-labeled using 3-bromo-1,1,1-trifluoroacetone. The PrOF NMR results reveal roles for the mobile α3 and L3 regions in the binding of both inhibitors and hydrolyzed β-lactam products to SPM-1...
March 2, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28249758/gaussian-process-based-independent-analysis-for-temporal-source-separation-in-fmri
#18
Ditte Høvenhoff Hald, Ricardo Henao, Ole Winther
Functional Magnetic Resonance Imaging (fMRI) gives us a unique insight into the processes of the brain, and opens up for analyzing the functional activation patterns of the underlying sources. Task-inferred supervised learning with restrictive assumptions in the regression set-up, restricts the exploratory nature of the analysis. Fully unsupervised independent component analysis (ICA) algorithms, on the other hand, can struggle to detect clear classifiable components on single-subject data. We attribute this shortcoming to inadequate modeling of the fMRI source signals by failing to incorporate its temporal nature...
February 26, 2017: NeuroImage
https://www.readbyqxmd.com/read/28249537/a-novel-design-of-a-scanning-probe-microscope-integrated-with-an-ultramicrotome-for-serial-block-face-nanotomography
#19
Anton E Efimov, Igor I Agapov, Olga I Agapova, Vladimir A Oleinikov, Alexey V Mezin, Michael Molinari, Igor Nabiev, Konstantin E Mochalov
We present a new concept of a combined scanning probe microscope (SPM)/ultramicrotome apparatus. It enables "slice-and-view" scanning probe nanotomography measurements and 3D reconstruction of the bulk sample nanostructure from series of SPM images after consecutive ultrathin sections. The sample is fixed on a flat XYZ scanning piezostage mounted on the ultramicrotome arm. The SPM measuring head with a cantilever tip and a laser-photodiode tip detection system approaches the sample for SPM measurements of the block-face surface immediately after the ultramicrotome sectioning is performed...
February 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28244322/impact-of-the-pd2spermine-chelate-on-osteosarcoma-metabolism-an-nmr-metabolomics-study
#20
Inês Lamego, M Paula M Marques, Iola F Duarte, Ana S Martins, Helena Oliveira, Ana M Gil
A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid metabolism. However, when in combination with doxorubicin and methotrexate, Pd2Spm induced strong metabolic deviations on lipids, choline compounds, amino acids, nucleotides, and compounds related to antioxidative mechanisms (e...
March 14, 2017: Journal of Proteome Research
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