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Red blood cell antigen biochemistry

Takashi Miyata, Hidehiro Tajima, Miki Hirata, Shin-Ichi Nakanuma, Isamu Makino, Hironori Hayashi, Katsunobu Oyama, Tomoharu Miyashita, Hiroyuki Takamura, Itasu Ninomiya, Sachio Fushida, Shoichi Iseki, Shin-Ichi Harada, Tomohiko Wakayama, Tetsuo Ohta
BACKGROUND AND AIM: Sinusoidal obstruction syndrome (SOS) is a serious drug-induced liver injury. However, the pathophysiology of the disease remains unclear. This study investigated the effects of cilostazol (CZ), a phosphodiesterase III inhibitor, in a monocrotaline (MCT)-induced rat model of SOS. METHODS: Male Wistar rats were administrated MCT to induce SOS. Rats were divided into control, MCT, and MCT+CZ groups. In the MCT+CZ group, CZ was administered at 48 h, 24 h, and 30 min prior to and 8 h and 24 h after MCT administration...
September 28, 2017: Journal of Gastroenterology and Hepatology
D Rose Ewald, Susan C J Sumner
Associations between blood type and disease have been studied since the early 1900s when researchers determined that antibodies and antigens are inherited. In the 1950s, the chemical identification of the carbohydrate structure of surface antigens led to the understanding of biosynthetic pathways. The blood type is defined by oligosaccharide structures, which are specific to the antigens, thus, blood group antigens are secondary gene products, while the primary gene products are various glycosyltransferase enzymes that attach the sugar molecules to the oligosaccharide chain...
November 2016: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
Max M Gong, Brendan D Macdonald, Trung Vu Nguyen, Kinh Van Nguyen, David Sinton
In this paper, we present a low cost and equipment-free blood filtration device capable of producing plasma from blood samples with mL-scale capacity and demonstrate its clinical application for hepatitis B diagnosis. We report the results of in-field testing of the device with 0.8-1 ml of undiluted, anticoagulated human whole blood samples from patients at the National Hospital for Tropical Diseases in Hanoi, Vietnam. Blood cell counts demonstrate that the device is capable of filtering out 99.9% of red and 96...
2013: Biomicrofluidics
A Gázquez, J Martín de Las Mulas, Y Millán Ruiz, A García, A J Masot, E Redondo
This paper reports on a canine angiosarcoma, presenting as an "undifferentiated metastasizing tumor". A 14-year-old female Cocker Spaniel was referred to the University of Extremadura Veterinary Clinic for clinical examination after suffering rapid deterioration, with chronic cough, anorexia and cachexia. One week after clinical examination, the dog died of right congestive heart failure and ventricular arrhythmia. Blood counts revealed lymphopenia and platelet depletion. The biochemistry profile was within normal limits, except for a drop in blood urea nitrogen...
2012: Histology and Histopathology
Sisse R Ostrowski, Anne Marie Sørensen, Nis A Windeløv, Anders Perner, Karen-Lise Welling, Michael Wanscher, Claus F Larsen, Pär I Johansson
BACKGROUND: The level of soluble vascular endothelial growth factor receptor 1 (sVEGFR1) is increased in sepsis and strongly associated with disease severity and mortality. Endothelial activation and damage contribute to both sepsis and trauma pathology. Therefore, this study measured sVEGFR1 levels in trauma patients upon hospital admission hypothesizing that sVEGFR1 would increase with higher injury severity and predict a poor outcome. METHODS: Prospective observational study of 80 trauma patients admitted to a Level I Trauma Centre...
2012: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
Abbasali Pourazar
Landsteiner and his colleagues demonstrated that human beings could be classified into four groups depending on the presence of one (A) or another (B) or both (AB) or none (O) of the antigens on their red cells. The number of the blood group antigens up to 1984 was 410. In the next 20 years, there were 16 systems with 144 antigens and quite a collection of antigens waiting to be assigned to systems, pending the discovery of new information about their relationship to the established systems. The importance of most blood group antigens had been recognized by immunological complications of blood transfusion or pregnancies; their molecular structure and function however remained undefined for many decades...
January 2007: Asian Journal of Transfusion Science
Eiji Hosoi
The ABO blood group was discovered in 1900 by Austrian scientist, Karl Landsteiner. At present, the International Society of Blood Transfusion (ISBT) approves as 29 human blood group systems. The ABO blood group system consists of four antigens (A, B, O and AB). These antigens are known as oligosaccharide antigens, and widely expressed on the membranes of red cell and tissue cells as well as, in the saliva and body fluid. The ABO blood group antigens are one of the most important issues in transfusion medicine to evaluate the adaptability of donor blood cells with bone marrow transplantations, and lifespan of the hemocytes...
August 2008: Journal of Medical Investigation: JMI
P W Kuchel
A critical analysis of the discovery of the first water channel protein (later called aquaporin 1) has been performed. In 1986 Benga's group in Cluj-Napoca, Romania, published in Biochemistry, a US-based journal, the results of experiments that provided the first visual and tangible evidence that the very rapid water exchange that occurs through the membranes of the human red blood cell (RBC) is mediated by a particular protein or small group of proteins. Benga and co-workers did first see bands in a gel that corresponded to water transporters, and were the first to do so...
2006: Cellular and Molecular Biology
A Corato, G Mazza, A S Hale, R N Barker, M J Day
Despite the clinical significance of the canine blood group antigens, relatively little is known of the biochemistry of these molecules. In this study the canine blood group antigens DEA (dog erythrocyte antigen) 1.2, 4 and 7 were immunoprecipitated from red blood cells (RBC) bearing the corresponding blood group, and molecular weights of 85 kD (DEA 1.2), 32-40 kD (DEA 4) and 53-66 kD (DEA 7) assigned. DEA 1.2 and DEA 4 each appeared as a single band, whereas DEA 7 comprised three distinct bands (53, 58 and 66 kD)...
November 1997: Veterinary Immunology and Immunopathology
M E Griot-Wenk, M B Callan, M L Casal, A Chisholm-Chait, S L Spitalnik, D F Patterson, U Giger
OBJECTIVE: To assess the genetics, frequency, and biochemistry of the AB blood type in cats. ANIMALS: Domestic shorthair and purebred cats in a breeding colony and privately owned catteries and blood samples from a large feline blood typing laboratory. PROCEDURES: Samples from cats with blood type AB were selected from the feline blood typing laboratory at the university. Breeding experiments and family studies were used for the genetic analysis of cats with blood type AB...
October 1996: American Journal of Veterinary Research
M H Pinard, B G Hepkema
Serology and biochemistry were used to identify major histocompatibility complex (MHC) types in chicken lines selected for high and low antibody response to sheep red blood cells. Serological typing was performed by direct haemagglutination, using antisera obtained by erythrocyte alloimmunization within the lines. Four serotypes were identified, called preliminarily B114, B119, B121 and B124. Subsequently, these types were characterized for their B-G and B-F products by biochemical analysis, using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and one-dimensional IEF (isoelectric focusing) respectively...
December 1993: Veterinary Immunology and Immunopathology
J P Cartron
The Rh blood group antigens (D, Cc and Ee series) are carried by a family of non glycosylated hydrophobic transmembrane proteins of 30-32 kDa which are missing from the red cells of rare Rhnull individuals that express several membrane defects. The structure of these proteins has been deduced from cDNA cloning and studies have shown that the Rh proteins are erythroid specific and share no sequence homology with any known protein. The RhD and non-D proteins exhibit 92% sequence identity and their predicted membrane topology is similar as most of the molecules appear to reside between the leaflets of the phospholipid bilayer with only short hydrophilic loops connecting the twelve putative transmembrane helices...
December 1994: Blood Reviews
N Rao, M Udani, J Nelson, M E Reid, M J Telen
BACKGROUND: The high-frequency Hy and Gya antigens have been shown to reside on the same protein. Gy(a-) Hy-negative red cells are also Do(a-b-). A mouse monoclonal antibody, 5B10, was produced with specificity related to the human Gregory, Holley, and Dombrock blood group antigens. STUDY DESIGN AND METHODS: The antibody reacted in direct hemagglutination assays, and its specificity was investigated by radioimmunoassay, inhibition assay, and Western blotting. RESULTS: The 5B10 antibody failed to bind to abnormal paroxysmal nocturnal hemoglobinuria red cells and human erythroleukemia cell line K562, but it was weakly reactive with HEL cells...
June 1995: Transfusion
P Bertagna, S Cohen, Q M Geiman, J Haworth, E Koenigk, W H Richards, P I Trigg
The study of the biochemistry, physiology, and immunology of plasmodia has been restricted by the difficulty of maintaining the parasites in isolation from the host. Some success has been achieved in cultivating them in vitro, using tissue cultures and chick embryo techniques to study exoerythrocytic states and the sporogonic cycle, but no completely successful method has been found for studying the asexual and sexual stages of plasmodia in circulating red blood cells. The relative slowness with which techniques for continuous in vitro cultivation have been developed is the result of inadequate knowledge of the biochemistry of the parasites and of the blood and its constituents...
1972: Bulletin of the World Health Organization
H Fukushima, M Ota, I Yonemura, H Hasekura, T Ishimori
Since Rh: -29, 38 (- - -/- - -, rh) phenotype of the Rh blood groups (--- in text) revealed unusual red cells, such as stomatocytes and microspherocytes and the relatively shortened half life of 17 days, red cell membrane proteins from Rh + (D), Rh - (d) and --- were compared by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No differences were observed among the patterns of the reduced and non-reduced membrane proteins from Rh+, Rh- and --- red cells. Two-dimensional gel electrophoresis of --- red cell membrane proteins also revealed a pattern similar to Rh+ and Rh- red cell membrane proteins...
1985: Comparative Biochemistry and Physiology. B, Comparative Biochemistry
H H Sonneborn, M Ernst
The M/N-blood-group system is very suitable for investigating the reactivity of monoclonal antibodies because the antigenic structure seems to be very dependent on influences of the microenvironment, whereas on the other hand the biochemistry of these antigens is very well known. The distribution of these antigens within the population makes this system very interesting for forensic investigations and therefore one needs specific reagents with constant quality. We have immunized BALB/c mice with whole red blood cells and obtained a series of monoclonal antibodies...
1987: Developments in Biological Standardization
V Pausch, P Speiser, U Michl, P Höcker, C P Engelfriet
The extremely rare phenotypes p, P1k and P2k (0.0005-0.0006%) of the blood group P system are usually found in consanguinous families. In the serum of these persons haemolytic antibodies with the specificity anti-PP1Pk and anti-P are found, causing severe haemolytic reactions after transfusion of incompatible blood. Because of their rarity it is difficult to find compatible blood donors. The antibodies are also associated with abortion early in pregnancy. Since 1948 at the "Institut für Blutgruppenserologie der Universität Wien" 4 persons of the phenotype p and 3 of the type P2k were observed in altogether 5 families...
February 2, 1990: Wiener Klinische Wochenschrift
W Graninger, A Poschmann, K Fischer, I Schedl-Giovannoni, H Hörandner, K Klaushofer
With help of immunoflorescence, best with anti-AHP from Helix pomatia, a stippled structure could be demonstrated on the patient"s red blood cells. Thus an "A-like" receptor could be detected on the erythrocyte membrane of this group O patient. The reactive antigen was proved not to be a crypt antigen exposed by the action of neuraminidase. The same stippled fluorescence with antiAhp was observed on the red blood cells of a patient suffering from hemolytic anemia induced by influnza A2 virus. In this case this virus was shown not to be responsible for polyagglutination...
1977: Vox Sanguinis
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