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https://www.readbyqxmd.com/read/28725337/complete-genome-sequence-of-leuconostoc-suionicum-dsm-20241-t-provides-insights-into-its-functional-and-metabolic-features
#1
Byung Hee Chun, Se Hee Lee, Hye Hee Jeon, Dong-Woon Kim, Che Ok Jeon
The genome of Leuconostoc suionicum DSM 20241(T) (=ATCC 9135(T) = LMG 8159(T) = NCIMB 6992(T)) was completely sequenced and its fermentative metabolic pathways were reconstructed to investigate the fermentative properties and metabolites of strain DSM 20241(T) during fermentation. The genome of L. suionicum DSM 20241(T) consists of a circular chromosome (2026.8 Kb) and a circular plasmid (21.9 Kb) with 37.58% G + C content, encoding 997 proteins, 12 rRNAs, and 72 tRNAs. Analysis of the metabolic pathways of L...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28722776/esterified-trehalose-analogs-protect-mammalian-cells-from-heat-shock
#2
Jack T Bragg, Hannah K D'Ambrosio, Timothy J Smith, Caroline A Gorka, Faraz A Khan, Joshua T Rose, Andrew J Rouff, Terence S Fu, Brittany J Bisnett, Michael Boyce, Sudhir Khetan, Margot G Paulick
Trehalose is a disaccharide that is produced by many organisms to better enable them to survive environmental stresses, including heat, cold, desiccation, and reactive oxygen species. Mammalian cells do not naturally biosynthesize trehalose; however, when introduced into mammalian cells, trehalose provides protection from the damages associated with freezing and drying. One of the major difficulties in using trehalose as a cellular protectant for mammalian cells is the delivery of this disaccharide into the intracellular environment; mammalian cell membranes are impermeable to the hydrophilic sugar trehalose...
July 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28722316/the-mincle-ligand-trehalose-dibehenate-differentially-modulates-m1-like-and-m2-like-macrophage-phenotype-and-function-via-syk-signaling
#3
Kristel Kodar, Jacquie L Harper, Melanie J McConnell, Mattie S M Timmer, Bridget L Stocker
INTRODUCTION: Macrophages play a significant role in the progression of diseases, such as cancer, making them a target for immune-modulating agents. Trehalose dibehenate (TDB) is known to activate M1-like macrophages via Mincle, however, the effect of TDB on M2-like macrophages, which are found in the tumor microenvironment, has not been studied. METHODS: qRT-PCR, flow cytometry, cytokine ELISA, and Western Blotting were used to study the effect of TDB on GM-CSF and M-CSF/IL-4 derived bone marrow macrophages (BMMs) from C57BL/6 and Mincle(-/-) mice...
July 19, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/28721580/gene-expression-profiles-of-the-thermotolerant-yeast-saccharomyces-cerevisiae-strain-kku-vn8-during-high-temperature-ethanol-fermentation-using-sweet-sorghum-juice
#4
Atiya Techaparin, Pornthap Thanonkeo, Preekamol Klanrit
OBJECTIVES: To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS: The maximal ethanol concentration obtained by S. cerevisiae KKU-VN8 using SSJ at 40 °C was 66.6 g/l, with a productivity of 1.39 g/l/h and a theoretical ethanol yield of 81%...
July 18, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28713776/modulation-of-trehalose-dimycolate-and-immune-system-by-rv0774c-protein-enhanced-the-intracellular-survival-of-mycobacterium-smegmatis-in-human-macrophages-cell-line
#5
Arbind Kumar, Varinder Saini, Anjani Kumar, Jasbinder Kaur, Jagdeep Kaur
Mycobacterium tuberculosis Rv0774c protein was reported previously to express under stress conditions. Therefore, Rv0774c gene was cloned and expressed in Mycobacterium smegmatis, a surrogate host, to determine its role in bacterial persistence and immune modulation in natural environment. The bacterial colonies expressing Rv0774c (Ms_rv0774c) were larger, smoother, more moist, and flatter than the control ones (Ms_ve). Enhanced survival of Ms_rv0774c after treatment with streptomycin was observed when compared with control...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28710552/the-african-froghopper-ptyelus-flavescens-suborder-cicadomorpha-contains-two-novel-and-one-known-peptides-of-the-adipokinetic-hormone%C3%A2-akh-family-structure-function-and-comparison-with-aphid-akh-suborder-sternorrhyncha
#6
Gerd Gäde, Petr Šimek, Heather G Marco
The rationale of "green pesticides" in food security is to use information about endogenous hormones of pest insects to make peptide mimetics that will act against the pest insects to alter their behaviour or physiology, while taking care not to harm beneficial insects or other organisms in the food chain. Such "green" insecticides are designed thus, on the basis of neuropeptide ligand-receptor interaction and it is of paramount interest to have finally a mimetic at hand that is harmful only to pest insects...
July 14, 2017: Amino Acids
https://www.readbyqxmd.com/read/28706236/metabolomic-differential-analysis-of-interspecific-interactions-among-white-rot-fungi-trametes-versicolor-dichomitus-squalens-and-pleurotus-ostreatus
#7
Feng Luo, Zixuan Zhong, Li Liu, Yasuo Igarashi, Deti Xie, Nannan Li
Interspecific fungal antagonism occurred commonly in the interaction zone of different white rot fungi. This competitive interaction could markedly influence the metabolic pathway of intracellular metabolites, which was associated with the fungal morphology change and growth restriction. So far, it remains unknown on intracellular metabolite regulation during fungal competitive interaction. Herein, we performed the metabolomic analysis of the in vivo metabolite changes during competitive interaction between each two of the three white rot fungi Trametes versicolor, Pleurotus ostreatus and Dichomitus squalens and identified differential metabolites in the interaction zone compared to each two isolates...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28704500/transcriptomic-and-metabolomic-profiles-of-chinese-citrus-fly-bactrocera-minax-diptera-tephritidae-along-with-pupal-development-provide-insight-into-diapause-program
#8
Jia Wang, Huan Fan, Ke-Cai Xiong, Ying-Hong Liu
The Chinese citrus fly, Bactrocera minax (Enderlein), is a devastating citrus pest in Asia. This univoltine insect enters obligatory pupal diapause in each generation, while little is known about the course and the molecular mechanisms of diapause. In this study, the course of diapause was determined by measuring the respiratory rate throughout the pupal stage. In addition, the variation of transcriptomic and metabolomic profiles of pupae at five developmental stages (pre-, early-, middle-, late-, and post-diapause) were evaluated by next-generation sequencing technology and 1H nuclear magnetic resonance spectroscopy (NMR), respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28702592/inhibition-of-gnnqqny-prion-peptide-aggregation-by-trehalose-a-mechanistic-view
#9
Nidhi Katyal, Shashank Deep
Deposition of amyloid fibrils is the seminal event in the pathogenesis of numerous neurodegenerative diseases. The formation of this amyloid assembly is the manifestation of a cascade of structural transitions including toxic oligomer formation in the early stages of aggregation. Thus a viable therapeutic strategy involves the use of small molecular ligands to interfere with this assembly. In this perspective, we have explored the kinetics of aggregate formation of the fibril forming GNNQQNY peptide fragment from the yeast prion protein SUP35 using multiple all atom MD simulations with explicit solvent and provided mechanistic insights into the way trehalose, an experimentally known aggregation inhibitor, modulates the aggregation pathway...
July 12, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28701150/physiological-roles-of-sigma-factor-sigd-in-corynebacterium-glutamicum
#10
Hironori Taniguchi, Tobias Busche, Thomas Patschkowski, Karsten Niehaus, Miroslav Pátek, Jörn Kalinowski, Volker F Wendisch
BACKGROUND: Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. RESULTS: In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated...
July 12, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28696506/preparation-of-acid-resistant-microcapsules-with-shell-matrix-structure-to-enhance-stability-of-streptococcus-thermophilus-iffi-6038
#11
Huan Bin Zhou, Jiashu Chen, Shunyi Li, Jianpan Zhang, Chun E Zhu, Hao Ran, Meihua Luo, Xin Pan, Haiyan Hu, Chuanbin Wu
Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Streptococcus thermophilus IFFI 6038 (IFFI 6038) cells were mixed with trehalose and alginate to fabricate microcapsules using extrusion...
July 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28694566/antifungal-resistance-metabolic-routes-as-drug-targets-and-new-antifungal-agents-an-overview-about-endemic-dimorphic-fungi
#12
REVIEW
Juliana Alves Parente-Rocha, Alexandre Melo Bailão, André Correa Amaral, Carlos Pelleschi Taborda, Juliano Domiraci Paccez, Clayton Luiz Borges, Maristela Pereira
Diseases caused by fungi can occur in healthy people, but immunocompromised patients are the major risk group for invasive fungal infections. Cases of fungal resistance and the difficulty of treatment make fungal infections a public health problem. This review explores mechanisms used by fungi to promote fungal resistance, such as the mutation or overexpression of drug targets, efflux and degradation systems, and pleiotropic drug responses. Alternative novel drug targets have been investigated; these include metabolic routes used by fungi during infection, such as trehalose and amino acid metabolism and mitochondrial proteins...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28693953/cryopreservation-of-luciola-praeusta-kiesenwetter-coleoptera-lampyridae-embryos-by-vitrification
#13
Kang Li, Xuanli Chen, Xiaoling Song, Xi Wu, Yi Xian
There is an urgent need to preserve the ever-decreasing number of different species of fireflies all over the world. We sought to develop a vitrification procedure to cryopreserve the firefly embryos. The late stages of Luciola praeusta Kiesenwetter embryos were collected. Several impermeable and permeable protectants with various concentrations in different mediums (TNM-FH insect medium, Grace's medium, Dulbecco's Modification of Eagle's Medium (DMEM) and Dulbecco's Phosphate-Buffered Saline (DPBS)) was used...
July 7, 2017: Cryobiology
https://www.readbyqxmd.com/read/28693684/clostridium-tepidum-sp-nov-a-close-relative-of-clostridium-sporogenes-and-clostridium-botulinum-group-i
#14
Anatoly P Dobritsa, Kirthi K Kutumbaka, Kirsten Werner, Martin Wiedmann, Aaron Asmus, Mansour Samadpour
Obligately anaerobic, Gram-stain-positive, spore-forming bacteria indistinguishable by pulsed-field gel electrophoresis were isolated from non-dairy protein shakes in bloated bottles. One of the isolates, strain IEH 97212T, was selected for further study. The strain was closely related to Clostridium sporogenes and Clostridium botulinum Group 1 based on 16S rRNA gene sequence similarities. Phylogenetic analysis also showed that strain IEH 97212T and strain PE (=DSM 18688), a bacterium isolated from solfataric mud, had identical 16S rRNA gene sequences...
July 10, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28692243/biosynthesis-of-astrocytic-trehalose-regulates-neuronal-arborisation-in-hippocampal-neurons
#15
Giuseppe Martano, Laura Gerosa, Ilaria Prada, Giulia Garrone, Vittorio Krogh, Claudia Verderio, Maria Passafaro
Trehalose is a non-reducing disaccharide that has recently attracted much attention because of its ability to inhibit protein aggregation, induce autophagy, and protect against dissections and strokes. In vertebrates, the biosynthesis of trehalose was long considered absent due to the lack of annotated genes involved in this process. In contrast, trehalase (TreH), which is an enzyme required for the cleavage of trehalose, is known to be conserved and expressed. Here, we show that trehalose is present as an endogenous metabolite in the rodent hippocampus...
July 10, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28691665/cold-stress-response-during-the-stationary-growth-phase-of-antarctic-and-temperate-climate-penicillium-strains
#16
Jeni G Miteva-Staleva, Ekaterina T Krumova, Spassen V Vassilev, Maria B Angelova
Cold-induced oxidative stress during the aging of three Penicillium strains (two Antarctic and one from a temperate region) in stationary culture was documented and demonstrated a significant increase in the protein carbonyl content, the accumulation of glycogen and trehalose, and an increase in the activities of antioxidant enzymes (superoxide dismutase and catalase). The cell response to a temperature downshift depends on the degree of stress and the temperature characteristics of the strains. Our data give further support for the role of oxidative stress in the aging of fungi in stationary cultures...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28689435/effect-of-molecular-weight-and-ratio-of-poly-ethylene-glycols-derivatives-in-combination-with-trehalose-on-stability-of-freeze-dried-igg
#17
Amir Hossein Mohammad Zadeh, Abdolhosein Rouholamini Najafabadi, Alireza Vatanara, Homa Faghihi, Kambiz Gilani
The influence of PEG (poly ethylene glycol) at different molecular weights and ratios was studied on the stability of freeze- dried IgG (immune globulin G). PEGs (600 to 4000 Dalton) at concentrations of 0.5 and 5% W/V were applied in the presence of 40 and 60% W/W of trehalose to prepare freeze- dried IgG formulations. Size- exclusion chromatography, Infra- red spectroscopy, Differential scanning calorimetery and Gel electrophoresis were performed to characterize lyophilized samples. Pure IgG demonstrated the highest aggregation of 5...
July 10, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28678828/a-technique-for-lyopreservation-of-clostridium-ljungdahlii-in-a-biocomposite-matrix-for-co-absorption
#18
Mark J Schulte, Jason Solocinski, Mian Wang, Michelle Kovacs, Ryan Kilgore, Quinn Osgood, Lukas Underwood, Michael C Flickinger, Nilay Chakraborty
A system capable of biocatalytic conversion of distributed sources of single carbon gases such as carbon monoxide into hydrocarbons can be highly beneficial for developing commercially viable biotechnology applications in alternative energy. Several anaerobic bacterial strains can be used for such conversion. The anaerobic carbon monoxide-fixing bacteria Clostridium ljungdahlii OTA1 is a model CO assimilating microorganism that currently requires cryogenic temperature for storage of the viable strains. If these organisms can be stabilized and concentrated in thin films in advanced porous materials, it will enable development of high gas fraction, biocomposite absorbers with elevated carbon monoxide (CO) mass transfer rate, that require minimal power input and liquid, and demonstrate elevated substrate consumption rate compared to conventional suspended cell bioreactors...
2017: PloS One
https://www.readbyqxmd.com/read/28677124/myba-a-transcription-factor-involved-in-conidiation-and-conidial-viability-of-the-human-pathogen-aspergillus-fumigatus
#19
Isabel Valsecchi, Özlem Sarikaya Bayram, Joanne Wong Sak Hoi, Laetitia Muszkieta, John Gibbons, Marie-Christine Prevost, Adeline Mallet, Jacomina Krijnse-Locker, Oumaima Ibrahim-Granet, Isabelle Mouyna, Paul Carr, Michael Bromley, VishuKumar Aimanianda, Y Jae-Hyuk Yu, Antonis Rokas, Gerhard Braus, Cosmin Saveanu, Özgür Bayram, Jean Paul Latgé
Aspergillus fumigatus, a ubiquitous human fungal pathogen, produces asexual spores (conidia), which are the main mode of propagation, survival, and infection of this human pathogen. In this study, we present the molecular characterization of a novel regulator of conidiogenesis and conidial survival called MybA because the predicted protein contains a Myb DNA binding motif. Cellular localisation of the MybA::Gfp fusion and immunoprecipitation of the MybA::Gfp or MybA::3xHa protein showed that MybA is localised to the nucleus...
July 5, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28673845/trehalose-radial-networks-protect-renilla-luciferase-helical-layers-against-thermal-inactivation
#20
Zahra Liyaghatdar, Rahman Emamzadeh, Sayed Mohammad Mahdi Rasa, Mahboobeh Nazari
Renilla luciferase (Rluc) from Renilla reniformis is an appropriate protein reporter for the detection of specific molecular targets due to its bioluminescent feature, although its relatively low stability limits the application. To investigate the effects of trehalose and sucrose as chemical chaperones on the kinetic stability of Rluc, we assayed the activity of the enzyme in the presence of these additives at high temperatures and to comprehend the mechanism of stability, molecular dynamic (MD) simulation was carried out...
June 30, 2017: International Journal of Biological Macromolecules
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