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https://www.readbyqxmd.com/read/27925009/characteristics-and-fertility-of-sumatran-tiger-spermatozoa-cryopreserved-with-different-sugars
#1
Ni Wayan Kurniani Karja, Mokhamad Fahrudin, Mohamad Agus Setiadi, Ligaya Ita Tumbelaka, Retno Sudarwati, Yohana Tri Hastuti, Bongot Huas Mulia, Ardyta Widianti, Keni Sultan, Tsukasa Terazono, Zhao Namula, Masayasu Taniguchi, Fuminori Tanihara, Tatsuya Takemoto, Kazuhiro Kikuchi, Yoko Sato, Takeshige Otoi
BACKGROUND: Cryopreservation of semen is one of the most important methods for the preservation of endangered tigers. OBJECTIVE: This study evaluated the effects of sugar supplementation on the cryosurvival of spermatozoa from Sumatran tigers (Panthera tigris sumatrae). MATERIALS AND METHODS: The post-thaw characteristics and fertility of spermatozoa cryopreserved with different sugars (glucose, lactose, and trehalose) were evaluated using heterologous in-vitro fertilisation with cat oocytes...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27924299/data-on-the-positive-synergic-action-of-dimethylacetamide-and-trehalose-on-quality-of-cryopreserved-chicken-sperm
#2
Fabio Mosca, Manuela Madeddu, Ahmad Abdel Sayed, Luisa Zaniboni, Nicolaia Iaffaldano, Silvia Cerolini
This data article contains supporting information regarding the research article entitled "Combined effect of permeant and non-permeant cryoprotectants on the quality of frozen/thawed chicken sperm"(Mosca et. al., 2016) [1]. The combined effect of the permeant cryoprotectants agent dimethylacetamide and the non-permeant cryoprotectants agent trehalose on the quality of frozen-thawed chicken semen was assessed. In particular, the quantitative dimethylacetamide/trehalose ratio was investigated freezing semen samples according to the following treatments: trehalose 0...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27923067/chronological-lifespan-in-yeast-is-dependent-on-the-accumulation-of-storage-carbohydrates-mediated-by-yak1-mck1-and-rim15-kinases
#3
Lu Cao, Yingzhi Tang, Zhenzhen Quan, Zhe Zhang, Stephen G Oliver, Nianshu Zhang
Upon starvation for glucose or any other macronutrient, yeast cells exit from the mitotic cell cycle and acquire a set of characteristics that are specific to quiescent cells to ensure longevity. Little is known about the molecular determinants that orchestrate quiescence entry and lifespan extension. Using starvation-specific gene reporters, we screened a subset of the yeast deletion library representing the genes encoding 'signaling' proteins. Apart from the previously characterised Rim15, Mck1 and Yak1 kinases, the SNF1/AMPK complex, the cell wall integrity pathway and a number of cell cycle regulators were shown to be necessary for proper quiescence establishment and for extension of chronological lifespan (CLS), suggesting that entry into quiescence requires the integration of starvation signals transmitted via multiple signaling pathways...
December 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27922102/slc2a8-glut8-is-a-mammalian-trehalose-transporter-required-for-trehalose-induced-autophagy
#4
Allyson L Mayer, Cassandra B Higgins, Monique R Heitmeier, Thomas E Kraft, Xia Qian, Jan R Crowley, Krzysztof L Hyrc, Wandy L Beatty, Kevin E Yarasheski, Paul W Hruz, Brian J DeBosch
Trehalose is a disaccharide demonstrated to mitigate disease burden in multiple murine neurodegenerative models. We recently revealed that trehalose rapidly induces hepatic autophagy and abrogates hepatic steatosis by inhibiting hexose transport via the SLC2A family of facilitative transporters. Prior studies, however, postulate that intracellular trehalose is sufficient to induce cellular autophagy. The objective of the current study was to identify the means by which trehalose accesses the hepatocyte cytoplasm, and define the distal signaling mechanisms by which trehalose induces autophagy...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921099/thermoresponsive-microgels-containing-trehalose-as-soft-matrices-for-3d-cell-culture
#5
Małgorzata Burek, Sylwia Waśkiewicz, Anna Lalik, Sebastian Student, Tadeusz Bieg, Ilona Wandzik
A series of thermoresponsive glycomicrogels with trehalose in the cross-links or with trehalose in the cross-links and as pending moieties was synthesized. These materials were obtained by surfactant-free precipitation copolymerization of N-isopropylacrylamide and various amounts of trehalose monomers. The resultant particles showed a spherical shape and a submicrometer hydrodynamic size with a narrow size distribution. At 25 °C, glycomicrogels in solutions with physiological ionic strength formed stable colloids, which further gelled upon heating to physiological temperature forming a macroscopic hydrogel with an interconnected porous structure...
December 6, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27910874/comprehensive-evaluation-of-molecular-enhancers-of-the-isothermal-exponential-amplification-reaction
#6
Ellie Mok, Eugene Wee, Yuling Wang, Matt Trau
The exponential amplification reaction (EXPAR) is an emerging isothermal nucleic acid amplification method with high potential for molecular diagnostics due to its isothermal nature and high amplification efficiency. However, the use of EXPAR is limited by the high levels of non-specific amplification. Hence, methods that can improve the specificity of EXPAR are desired to facilitate its widespread adoption in practice. Herein, we proposed a strategy to improve EXPAR performance by using molecular enhancers...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27909048/the-ph-dependence-of-saccharides-influence-on-thermal-denaturation-of-two-model-proteins-supports-an-excluded-volume-model-for-stabilization-generalized-to-allow-for-intramolecular-electrostatic-interactions
#7
Ilyas Beg, Allen P Minton, Asimul Islam, Md Imtaiyaz Hassan, Faizan Ahmad
The reversible thermal denaturation of apo alpha-lactalbumin and lysozyme was measured in the absence and presence of multiple concentrations of each of seven saccharides (glucose, galactose, fructose, sucrose, trehalose, raffinose and stachyose) at multiple pH values. It was observed that with increasing pH, the absolute stability of alpha-lactalbumin decreased while the stabilizing effect per mole of all saccharides increased, and that the absolute stability of lysozyme increased, while the stabilizing effect per mole of all saccharides decreased...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27908495/analysis-of-low-abundant-trehalose-6-phosphate-and-related-metabolites-in-medicago-truncatula-by-hydrophilic-interaction-liquid-chromatography-triple-quadrupole-mass-spectrometry
#8
Ana Teresa Mata, Tiago Filipe Jorge, João Ferreira, Maria do Rosário Bronze, Diana Branco, Pedro Fevereiro, Susana Araújo, Carla António
Trehalose-6-phosphate (T6P) is an important signaling metabolite involved in plant growth control that inhibits the sucrose nonfermenting-1-related protein kinase 1 (SnRK1), a key regulator of energy and carbon metabolism in plants. The quantification of T6P in plant tissues is fundamental to improve our understanding of sugar signaling and the links between plant growth and development in response to stress conditions. However, the almost undetectable levels of T6P together with the complex plant matrix and the presence of T6P isomers such as sucrose-6-phosphate (S6P), makes the detection of this metabolite challenging...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27905883/carbohydrate-metabolism-in-oenococcus-oeni-a-genomic-insight
#9
Alice Cibrario, Claire Peanne, Marine Lailheugue, Hugo Campbell-Sills, Marguerite Dols-Lafargue
BACKGROUND: Oenococcus oeni is the bacterial species that drives malolactic fermentation in most wines. Several studies have described a high intraspecific diversity regarding carbohydrate degradation abilities but the link between the phenotypes and the genes and metabolic pathways has been poorly described. RESULTS: A collection of 41 strains whose genomic sequences were available and representative of the species genomic diversity was analyzed for growth on 18 carbohydrates relevant in wine...
December 1, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27903647/the-production-and-utilization-of-gdp-glucose-in-the-biosynthesis-of-trehalose-6-phosphate-by-streptomyces-venezuelae
#10
Matías D Asencion Diez, Farzana Miah, Clare E M Stevenson, David M Lawson, Alberto A Iglesias, Stephen Bornemann
Trehalose-6-phosphate synthase OtsA from streptomycetes is unusual in that it uses GDP-glucose as the donor substrate rather than the more commonly used UDP-glucose. We now confirm that OtsA from Streptomyces venezuelae has such a preference for GDP-glucose and can utilise ADP-glucose to some extent too. A crystal structure of the enzyme shows that it shares twin Rossmann-like domains with the UDP-glucose-specific OtsA from Escherichia coli However, it is structurally more similar to Streptomyces hygroscopicus VldE, a GDP-valienol-dependent pseudo-glycosyltransferase enzyme...
November 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27902348/micropepsis-pineolensis-gen-nov-sp-nov-a-mildly-acidophilic-alphaproteobacterium-isolated-from-a-poor-fen-and-proposal-of-micropepsaceae-fam-nov-within-micropepsales-ord-nov
#11
Austin B Harbison, Laiken E Price, Michael D Flythe, Suzanna L Brauer
An obligately anaerobic, mildly acidophilic, fermentative alphaproteobacterium, designated strain CS4 T, was isolated from an acidic, oligotrophic (nutrient-poor) poor fen located near Pineola, NC, USA. Cultures contained Gram-negative staining, slightly curved, non-motile, non-spore forming, non-prosthecate rods (0.1-0.4 µm in diameter and 0.4-4 µm long, depending, in part, on the growth substrate). Growth optima were 35° C (range 15 - 35°C), pH 5.6 (range of 5.0 - 6.8), and with 0-50 mM added NaCl (range 0-100 mM added NaCl)...
November 21, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27895647/exogenous-trehalose-treatment-enhances-the-activities-of-defense-related-enzymes-and-triggers-resistance-against-downy-mildew-disease-of-pearl-millet
#12
Sharathchandra R Govind, Sudisha Jogaiah, Mostafa Abdelrahman, Hunthrike S Shetty, Lam-Son P Tran
In recent years, diverse physiological functions of various sugars are the subject of investigations. Their roles in signal transduction in plant responses to adverse biotic and abiotic stress conditions have become apparent, and growing scientific evidence has indicated that disaccharides like sucrose and trehalose mediate plant defense responses in similar way as those induced by elicitors against the pathogens. Trehalose is a well-known metabolic osmoregulator, stress-protectant and non-reducing disaccharide existing in a variety of organisms, including fungi, bacteria, and plants...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27895594/larval-exposure-to-chlorpyrifos-affects-nutritional-physiology-and-induces-genotoxicity-in-silkworm-philosamia-ricini-lepidoptera-saturniidae
#13
Moni K Kalita, Kishor Haloi, Dipali Devi
Chlorpyrifos is a most widely used organophosphate insecticide because of its cost effectiveness and degradable nature. However, this pesticide enters and contaminates the environment either by direct application, spray drifts or crop run off and shows adverse effect on the non-targeted organisms. Philosamia ricini (eri silkworm), one of the most exploited, domesticated and commercialized non mulberry silkworm is known for mass production of eri silk. The silkworm larvae get exposed to pesticide residues on the leaves of food plants...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27889398/recognition-of-mycobacterial-lipids-by-immune-receptors
#14
REVIEW
Eri Ishikawa, Daiki Mori, Sho Yamasaki
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), infects one-third of the world's population and causes 1.5 million deaths each year. The cell envelopes of mycobacteria comprise a wealth of unique glycolipids, including trehalose-6,6'-dimycolate (TDM), lipoarabinomannan (LAM), lipomannan (LM), and phosphatidylinositol (PI) mannosides (PIMs). These lipids are important modulators of the host immune responses during infection and in some cases have been used as adjuvants [e.g., complete Freund's adjuvant (CFA)]...
November 23, 2016: Trends in Immunology
https://www.readbyqxmd.com/read/27886438/self-assembly-of-bolaamphiphilic-molecules
#15
REVIEW
Prabhu Dhasaiyan, Bhagavatula L V Prasad
The current buzzword in science and technology is self-assembly and molecular self-assembly is one of the most prominent fields as far as research in chemical and biological sciences is concerned. Generally, self-assembly of molecules occurs through weak non-covalent interactions like hydrogen bonding, π-π stacking, hydrophobic effects, etc. Inspired by many natural systems consisting of self-assembled structures, scientists have been trying to understand their formation and mimic such processes in the laboratory to create functional "smart" materials, which respond to temperature, light, pH, electromagnetic field, mechanical stress, and/or chemical stimuli...
November 25, 2016: Chemical Record: An Official Publication of the Chemical Society of Japan ... [et Al.]
https://www.readbyqxmd.com/read/27885744/glutathione-in-combination-with-trehalose-has-supplementary-beneficial-effects-on-cryopreserved-red-deer-cervus-elaphus-sperm
#16
Yan Wang, Shude Dong
OBJECTIVES: In this study, we evaluated the effects of glutathione in combination with trehalose addition to semen extenders on the quality parameters of frozen-thawed red deer (cervus elaphus) spermatozoa. METHOD OF STUDY: The semen samples collected from six mature red deer once a week were diluted with Tris-egg yolk-based extenders. The diluted semen samples were supplemented with glutathione (8 mmol L(-1) ) and or trehalose (5%, w/v), cryopreserved, thawed and then subjected to sperm quality parameter evaluation...
November 25, 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27885726/cryopreservation-of-feline-oocytes-by-vitrification-using-commercial-kits-and-slush-nitrogen-technique
#17
L Fernandez-Gonzalez, K Jewgenow
Assisted reproductive techniques are a valuable tool for conservation breeding of endangered species. Cryopreservation methods are the basis of gamete banks, supporting genetic diversity preservation. Unfortunately, cryopreservation of feline oocytes is still considered an experimental technique. The aim of this study was to compare two commercial kits, with our protocol for vitrification of cat oocytes (IZW), which comprises a three-step method with ethylene glycol, DMSO, fetal calf serum, trehalose and Ficoll PM-70...
November 24, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27871371/effect-of-c-terminal-domain-truncation-of-thermus-thermophilus-trehalose-synthase-on-its-substrate-specificity
#18
Chang-Bae Cho, Da-Yeon Park, Soo-Bok Lee
The C-terminal domain of the three-domain-comprising trehalose synthase from Thermus thermophilus was truncated in order to study the effect on the enzyme's activity and substrate specificity. Compared with the wild-type (WT) enzyme, the two truncated enzymes (DM1 and DM2) showed lower maltose- and trehalose-converting activities and a different transglycosylation reaction mechanism. In the mutants, the glucose moiety cleaved from the maltose substrate was released from the enzyme and intercepted by external glucose oxidase, preventing the production of trehalose...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27867377/over-expression-of-the-mycobacterial-trehalose-phosphate-phosphatase-otsb2-results-in-a-defect-in-macrophage-phagocytosis-associated-with-increased-mycobacterial-macrophage-adhesion
#19
Hao Li, Mei Wu, Yan Shi, Babak Javid
Trehalose-6-phosphate phosphatase (OtsB2) is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth. We wished to determine the effects of OtsB2 expression on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-bacillus calmette-guerin (BCG) over-expressing OtsB2 were able to better survive in stationary phase. Over-expression of OtsB2 led to a decrease in phagocytosis but not survival in THP-1 macrophage-like cells, and this was not due to a decrease in general macrophage phagocytic activity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27864376/probing-function-and-structure-of-trehalose-6-phosphate-phosphatases-from-pathogenic-organisms-suggests-distinct-molecular-groupings
#20
Megan Cross, Romain Lepage, Siji Rajan, Sonja Biberacher, Neil D Young, Bo-Na Kim, Mark J Coster, Robin B Gasser, Jeong-Sun Kim, Andreas Hofmann
The trehalose biosynthetic pathway is of great interest for the development of novel therapeutics because trehalose is an essential disaccharide in many pathogens but is neither required nor synthesized in mammalian hosts. As such, trehalose-6-phosphate phosphatase (TPP), a key enzyme in trehalose biosynthesis, is likely an attractive target for novel chemotherapeutics. Based on a survey of genomes from a panel of parasitic nematodes and bacterial organisms and by way of a structure-based amino acid sequence alignment, we derive the topological structure of monoenzyme TPPs and classify them into 3 groups...
November 18, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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