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https://www.readbyqxmd.com/read/28551424/formulation-optimization-of-freeze-dried-long-circulating-liposomes-and-in-line-monitoring-of-the-freeze-drying-process-using-an-nir-spectroscopy-tool
#1
Bianca Sylvester, Alina Porfire, Pieter-Jan Van Bockstal, Sebastian Porav, Marcela Achim, Thomas DE Beer, Ioan Tomuță
The effect of lyoprotectant type and concentration on the stability of freeze-dried prednisolone sodium phosphate-loaded long-circulating liposomes (LCL-PLP) was investigated. Trehalose at a 5:1 carbohydrate to lipid molar ratio proved to be superior in maintaining the structural integrity and the permeability properties of the liposome bilayers, assuring the desired characteristics of the final product: a cake with a porous structure and easy to reconstitute, a similar size to the liposomes prior to freeze-drying, a high percent of encapsulated drug and a low residual moisture content...
May 24, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28551219/stabilization-of-beta-lactoglobulin-by-polyols-and-sugars-against-temperature-induced-denaturation-involves-diverse-and-specific-structural-regions-of-the-protein
#2
Alberto Barbiroli, Mauro Marengo, Dimitrios Fessas, Enzio Ragg, Stefano Renzetti, Francesco Bonomi, Stefania Iametti
Temperature sensitivity of bovine milk beta-lactoglobulin (BLG) was assessed in the presence/absence of non-reducing sugars (sucrose and trehalose) and polyols (glycerol and sorbitol). None of them affected the structural features of the protein at room temperature, where the only observed effect was an increased affinity towards hydrophobic probes in the presence of all co-solutes but glycerol. Although most of the observed effects in temperature-ramp experiments are due to entropic effects (fitting within the "preferential exclusion" theory of protein stabilization), this study indicates that each co-solute exhibit different efficacy at stabilizing specific regions of BLG, suggesting that each of them acts in a specific way on the solvent/protein system...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28543267/trehalose-sustains-a-higher-post-thaw-sperm-motility-than-sucrose-in-vitrified-human-sperm
#3
M Schulz, J Risopatrón, G Matus, E Pineda, C Rojas, V Isachenko, E Isachenko, R Sánchez
One of the cryopreservation methods that best preserves sperm function is vitrification. However, comparative studies have not been performed to evaluate the effect of nonpermeable cryoprotectors on sperm function for prolonged periods of time post-devitrification. These times are necessary, especially in in vitro fertilisation and intrauterine insemination, for gamete interaction and then fertilisation to occur, while maintaining motility to arrive at the fertilisation site. In this study, sucrose (.25 m) and trehalose (...
May 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28542282/expression-patterns-of-members-of-the-ethylene-signaling-related-gene-families-in-response-to-dehydration-stresses-in-cassava
#4
Meng Yun Ren, Ren Jun Feng, Hou Rui Shi, Li Fang Lu, Tian Yan Yun, Ming Peng, Xiao Guan, Heng Zhang, Jing Yi Wang, Xi Yan Zhang, Cheng Liang Li, Yan Jun Chen, Peng He, Yin Dong Zhang, Jiang Hui Xie
Drought is the one of the most important environment stresses that restricts crop yield worldwide. Cassava (Manihot esculenta Crantz) is an important food and energy crop that has many desirable traits such as drought, heat and low nutrients tolerance. However, the mechanisms underlying drought tolerance in cassava are unclear. Ethylene signaling pathway, from the upstream receptors to the downstream transcription factors, plays important roles in environmental stress responses during plant growth and development...
2017: PloS One
https://www.readbyqxmd.com/read/28537141/evaluation-of-acceptor-selectivity-of-lactococcus-lactis-ssp-lactis-trehalose-6-phosphate-phosphorylase-in-the-reverse-phosphorolysis-and-synthesis-of-a-new-sugar-phosphate
#5
Yodai Taguchi, Wataru Saburi, Ryozo Imai, Haruhide Mori
Trehalose 6-phosphate phosphorylase (TrePP), a member of glycoside hydrolase family 65, catalyzes the reversible phosphorolysis of trehalose 6-phosphate (Tre6P) with inversion of the anomeric configuration to produce β-d-glucose 1-phosphate (β-Glc1P) and d-glucose 6-phosphate (Glc6P). TrePP in Lactococcus lactis ssp. lactis (LlTrePP) is, alongside the phosphotransferase system, involved in the metabolism of trehalose. In this study, recombinant LlTrePP was produced and characterized. It showed its highest reverse phosphorolytic activity at pH 4...
May 24, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28535265/physiology-of-cold-hardiness-seasonal-fluctuations-and-cryoprotectant-contents-in-overwintering-adults-of-hypera-postica-coleoptera-curculionidae
#6
Mahdieh Saeidi, Saeid Moharramipour
The alfalfa weevil, Hypera postica (Gyllenhal), is widely distributed in various alfalfa-growing regions in the world. Adults enter the field gradually from hibernation places and overwinter in a nondiapausing state, exhibiting feeding, mating, and oviposition over autumn and winter whenever the weather permits. In this study, the variability of supercooling point (SCP) and cold hardiness in alfalfa weevil was investigated. Adults were collected monthly from alfalfa fields in Karaj, Iran, during autumn, winter, and spring in 2011-2012...
May 23, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28532497/improving-the-production-of-22-hydroxy-23-24-bisnorchol-4-ene-3-one-from-sterols-in-mycobacterium-neoaurum-by-increasing-cell-permeability-and-modifying-multiple-genes
#7
Liang-Bin Xiong, Hao-Hao Liu, Li-Qin Xu, Wan-Ju Sun, Feng-Qing Wang, Dong-Zhi Wei
BACKGROUND: The strategy of modifying the sterol catabolism pathway in mycobacteria has been adopted to produce steroidal pharmaceutical intermediates, such as 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC), which is used to synthesize various steroids in the industry. However, the productivity is not desirable due to some inherent problems, including the unsatisfactory uptake rate and the low metabolic efficiency of sterols. The compact cell envelope of mycobacteria is a main barrier for the uptake of sterols...
May 22, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28530656/metabolic-anticipation-in-mycobacterium-tuberculosis
#8
Hyungjin Eoh, Zhe Wang, Emilie Layre, Poonam Rath, Roxanne Morris, D Branch Moody, Kyu Y Rhee
Humans serve as both host and reservoir for Mycobacterium tuberculosis, making tuberculosis a theoretically eradicable disease. How M. tuberculosis alternates between host-imposed quiescence and sporadic bouts of replication to complete its life cycle, however, remains unknown. Here, we identify a metabolic adaptation that is triggered upon entry into hypoxia-induced quiescence but facilitates subsequent cell cycle re-entry. Catabolic remodelling of the cell surface trehalose mycolates of M. tuberculosis specifically generates metabolic intermediates reserved for re-initiation of peptidoglycan biosynthesis...
May 22, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28530586/characterization-of-textural-rheological-thermal-microstructural-and-water-mobility-in-wheat-flour-dough-and-bread-affected-by-trehalose
#9
Bo Peng, Youqian Li, Shiyong Ding, Jun Yang
The study aims to elucidate the effects of trehalose on the mechanical, thermal, and rheological properties of wheat flour dough and water distribution in bread. Texture profile analysis, DSC, farinograph, extensograph, and frequency sweep were applied in dough. The results from SEM revealed that the gluten film became less notable with the presence of trehalose. The kinetics of staling process, low-field (1)H NMR, and water-binding capacity were employed to characterize physicochemical properties of bread...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28529155/metabolic-insights-into-the-cold-survival-strategy-and-overwintering-of-the-common-cutworm-spodoptera-litura-fabricius-lepidoptera-noctuidae
#10
Wei Zhu, Huan Zhang, Qian Meng, Menglong Wang, Guiling Zhou, Xuan Li, Hongtuo Wang, Lin Miao, Qilian Qin, Jihong Zhang
The common cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae), is a destructive pest in Asia. Although overwintering in the field has not been reported for this species, their larvae are capable of long-term survival in fluctuating temperatures, i.e., 5°C (12h) plus 13°C (12h), if food is available. With an increase in climate change due to global warming and the widespread use of greenhouses, further understanding of their cold survival strategy is needed to predict and control their population in the future...
May 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28528092/structural-strength-analysis-of-amorphous-trehalose-maltodextrin-systems
#11
V A Maidannyk, B Nurhadi, Y H Roos
Fundamental knowledge of physical state of materials gives practically important information for food, biological and pharmaceutical industry. Based on Williams-Landel-Ferry (WLF) equation, the strength concept was introduced. This concept provides a simple parameter, S, to express resistance of solids to flow above the glass transition temperature. To develop this approach, miscible trehalose-maltodextrin (0:100; 20:80; 40:60; 60:40; 80:20 and 100:0) systems with different ratios of components were used in the present study...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28525711/protein-partial-molar-volumes-in-multi-component-solutions-from-the-perspective-of-inverse-kirkwood-buff-theory
#12
Cesar Calero-Rubio, Curtis M Strab, Gregory V Barnett, Christopher J Roberts
Inverse Kirkwood-Buff (KB) solution theory can be used to relate macroscopic quantities with molecular scale interactions and correlation functions, in the form of KB integrals. Protein partial specific volumes (<HAT>V</HAT>2) from high-precision density measurements can be used to quantify solvent-solute and solute-solute KB integrals. Currently, general expressions for <HAT>V</HAT>2 as a function of co-solute concentration (c3) have been provided for only binary and ternary solutions...
May 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28515463/enzyme-characteristics-of-pathogen-specific-trehalose-6-phosphate-phosphatases
#13
Megan Cross, Siji Rajan, Janine Chekaiban, Jake Saunders, Chloe Hamilton, Jeong-Sun Kim, Mark J Coster, Robin B Gasser, Andreas Hofmann
Owing to the key role of trehalose in pathogenic organisms, there has recently been growing interest in trehalose metabolism for therapeutic purposes. Trehalose-6-phosphate phosphatase (TPP) is a pivotal enzyme in the most prominent biosynthesis pathway (OtsAB). Here, we compare the enzyme characteristics of recombinant TPPs from five important nematode and bacterial pathogens, including three novel members of this protein family. Analysis of the kinetics of trehalose-6-phosphate hydrolysis reveals that all five enzymes display a burst-like kinetic behaviour which is characterised by a decrease of the enzymatic rate after the pre-steady state...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28513878/gene-cloning-expression-and-characterization-of-trehalose-6-phosphate-synthase-from-pleurotus-ostreatus
#14
Min Lei, Xiangli Wu, Jinxia Zhang, Hexiang Wang, Chenyang Huang
Trehalose-6-phosphate synthase (TPS; EC2.4.1.15) catalyzes the first step in trehalose synthesis, which involves transfer of glucose from uridine diphosphate glucose (UDPG) to glucose 6-phosphate (G6P) to form trehalose-6-phosphate. To determine the gene and enzymatic characteristics of TPS in Pleurotus ostreatus, we cloned and sequenced the cDNA of PoTPS1, which contains a 1665 bp open reading frame that encodes a 554-amino acid protein with a predicted molecular weight of 62.01 kDa. This gene was expressed in Escherichia coli BL21 and then the recombinant protein was purified and characterized...
May 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28512660/selective-protein-recognition-in-supported-lipid-bilayer-arrays-by-tailored-dual-mode-deep-cavitand-hosts
#15
Lizeth Perez, Magi Mettry, Samuel S Hinman, Samantha R Byers, Kristy S McKeating, Bethany G Caulkins, Quan Cheng, Richard J Hooley
Self-folding deep cavitands with variably functionalized upper rims are able to selectively immobilize proteins at a biomimetic supported lipid bilayer surface. The immobilization process takes advantage of the dual-mode binding capabilities of the hosts, combining a defined binding pocket with upper rim charged/H-bonding groups. A variety of proteins can be selectively immobilized at the bilayer interface, either via complementary charge/H-bonding interactions, cavity-based molecular recognition, or a combination of both...
May 17, 2017: Soft Matter
https://www.readbyqxmd.com/read/28509971/description-of-chryseobacterium-timonianum-sp-nov-isolated-from-a-patient-with-pneumonia
#16
Rita Abou Abdallah, Teresa Cimmino, Sophie Baron, Frédéric Cadoret, Caroline Michelle, Didier Raoult, Pierre-Edouard Fournier, Fadi Bittar
Using a polyphasic taxonomic strategy, an aerobic, Gram-negative, non-motile, yellow pigmented rod isolated from a sputum sample of a patient with pneumonia was characterised. This bacterial strain, designated G972(T), could not be identified by our systematic MALDI-TOF screening on a MicroFlex. This led to the sequencing of the 16S rRNA gene, which shows 98.57% sequence identity with that of Chryseobacterium indologenes 16777(T), the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species...
May 16, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28507063/the-mycobacterium-tuberculosis-mmpl11-cell-wall-lipid-transporter-is-important-for-biofilm-formation-intracellular-growth-and-non-replicating-persistence
#17
Catherine C Wright, Fong Fu Hsu, Eusondia Arnett, Jennifer L Dunaj, Patrick M Davidson, Sophia A Pacheco, Melanie J Harriff, David M Lewinsohn, Larry S Schlesinger, Georgiana E Purdy
The mycobacterial cell wall is crucial to the host-pathogen interface since it provides a barrier against antibiotics and the host immune response. In addition, cell wall lipids are mycobacterial virulence factors. The mycobacterial membrane protein large (MmpL) proteins are cell wall lipid transporters that are important for basic mycobacterial physiology and M. tuberculosis pathogenesis. MmpL3 and MmpL11 are conserved across pathogenic and nonpathogenic mycobacteria, consistent with an important role in the basic physiology of the bacterium...
May 15, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28500644/autophagy-stimulation-prevents-intestinal-mucosal-inflammation-and-ameliorates-murine-colitis
#18
Dulce C Macias-Ceja, Jesús Cosín-Roger, Dolores Ortiz-Masiá, Pedro Salvador, Carlos Hernández, Juan V Esplugues, Sara Calatayud, María D Barrachina
BACKGROUND AND POURPOSE: A defective autophagy is involved in the pathogenesis of inflammatory disorders such as IBD. Cross talk interactions between autophagy and inflammation have been reported and we analyse the effects of autophagy stimulators on murine colitis. EXPERIMENTAL APPROACH: Mice were treated with intrarectal administration of TNBS (3.5 mg/20 g BW) and body weight was measured every day and histological damage score analysed two or four days after treatment...
May 12, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28499875/natural-zwitterionic-l-carnitine-as-efficient-cryoprotectant-for-solvent-free-cell-cryopreservation
#19
Hongwen Zhai, Jing Yang, Jiamin Zhang, Chao Pan, Nana Cai, Yingnan Zhu, Lei Zhang
Organic solvents, such as dimethyl sulfoxide (DMSO) and glycerol, have been commonly used as cryoprotectants (CPAs) in cell cryopreservation. However, their cytotoxicity and need of complex freezing protocols have impeded their applications especially in clinical cell therapy and regenerative medicine. Trehalose has been explored as a natural CPA to cryopreserve cells, but its poor cell permeability frequently results in low cryopreservation efficacy. In this work, we presented that a natural zwitterionic molecule-l-carnitine-could serve as a promising CPA for solvent-free cryopreservation...
May 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28497975/adjuvants-based-on-synthetic-mycobacterial-cord-factor-analogues-biophysical-properties-of-neat-glycolipids-and-nano-self-assemblies-with-dda
#20
Rie S Kallerup, Henrik Franzyk, Mikkel L Schiøth, Sarah Justesen, Birte Martin-Bertelsen, Fabrice Rose, Cecilie M Madsen, Dennis Christensen, Karen S Korsholm, Anan Yaghmur, Camilla Foged
Synthetic mycobacterial cord factor analogues, e.g., trehalose 6,6'-dibehenate (TDB), are highly promising adjuvants due to their strong immunopotentiating capabilities, but their biophysical properties have remained poorly characterized. Here, we report the synthesis of an array of synthetic TDB analogues varying in acyl chain length, degree of acylation and headgroup display, which was subjected to biophysical characterization of neat non-dispersed self-assembled nanostructures in excess buffer and as aqueous dispersions with cationic dimethyldioctadecylammonium (DDA) bromide...
May 12, 2017: Molecular Pharmaceutics
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