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trehalose

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https://www.readbyqxmd.com/read/28822290/freeze-dryable-lipid-vesicles-with-size-tunability-and-high-encapsulation-efficiency-prepared-by-the-multiple-emulsification-solvent-evaporation-method
#1
Akihiko Suzuki, Takashi Kuroiwa, Kaname Horikoshi, Akihiko Kanazawa, Sosaku Ichikawa
We investigated the extent of potential applicability of our recently developed method for preparing lipid vesicles [T. Kuroiwa et al., J. Am. Oil Chem. Soc., 93 (2016) 421], designated as the multiple emulsification-solvent evaporation method, with the intention of controlling the vesicle diameter and achieving high entrapment efficiency for water-soluble compounds. Using this method, the diameter of lipid vesicles could be varied by selecting the methods for preparing the primary water-in-oil emulsion, which contained water droplets as templates for the internal water phases of lipid vesicles...
August 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28821765/the-protective-action-of-osmolytes-on-the-deleterious-effects-of-gamma-rays-and-atmospheric-pressure-plasma-on-protein-conformational-changes
#2
Pankaj Attri, Minsup Kim, Thapanut Sarinont, Eun Ha Choi, Hyunwoong Seo, Art E Cho, Kazunori Koga, Masaharu Shiratani
Both gamma rays and atmospheric pressure plasma are known to have anticancer properties. While their mechanism actions are still not clear, in some contexts they work in similar manner, while in other contexts they work differently. So to understand these relationships, we have studied Myoglobin protein after the treatment of gamma rays and dielectric barrier discharge (DBD) plasma, and analyzed the changes in thermodynamic properties and changes in the secondary structure of protein after both treatments. The thermodynamic properties were analyzed using chemical and thermal denaturation after both treatments...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818367/metabolic-features-involved-in-drought-stress-tolerance-mechanisms-in-peanut-nodules-and-their-contribution-to-biological-nitrogen-fixation
#3
Ana Laura Furlan, Eliana Bianucci, Stella Castro, Karl-Josef Dietz
Legumes belong to the most important crops worldwide. They increase soil fertility due their ability to establish symbiotic associations with soil microorganisms, known as rhizobia, capable of fixing nitrogen from the atmosphere. However, they are frequently exposed to abiotic stress conditions in particular drought. Such adverse conditions impair the biological nitrogen fixation (BNF) and depend largely on the legume. Therefore, two peanut cultivars with contrasting tolerance to drought, namely the more tolerant EC-98 and the sensitive Granoleico, were investigated to elucidate the relative contribution of BNF to the tolerance to drought...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28814124/comparative-metabolic-profiling-of-costus-speciosus-leaves-and-rhizomes-using-nmr-gc-ms-and-uplc-esi-ms-ms
#4
Ashish Kumar, Antim K Maurya, Gopi Chand, Vijai K Agnihotri
Costus speciosus had been used in oriental systems of medicines, to treat diverse ailments. The present study was focused on NMR, GC-MS and UPLC/ESI-MS/MS-based metabolic profiling of C. speciosus. This metabolic study resulted in the identification of 91 and quantification of 69 metabolites. Caffeic acid derivatives previously unreported in C. speciosus were also identified. High quantity of steroidal saponins namely methyl protogracillin (297.97 ± 0.07 mg/g dried wt.) and dioscin (158.72 ± 0.27 mg/g dried wt...
August 17, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28809501/microencapsulated-solid-lipid-nanoparticles-as-a-hybrid-platform-for-pulmonary-antibiotic-delivery
#5
Diana P Gaspar, Maria Manuela Gaspar, Carla V Eleutério, Ana Grenha, Mateo Blanco, Lídia M D Gonçalves, Pablo Taboada, António J Almeida, Carmen Remuñán-López
Solid lipid nanoparticles (SLN) containing rifabutin (RFB), with pulmonary administration purposes, were developed through a technique that avoids the use of organic solvents or sonication. To facilitate their pulmonary delivery, the RFB-loaded SLN were included in microspheres of appropriate size using suitable excipients (mannitol and trehalose) through a spray-drying technique. Confocal analysis microscopy showed that microspheres are spherical and that SLN are efficiently microencapsulated and homogeneously distributed throughout the microsphere matrices...
August 15, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28809106/structural-characteristics-and-function-of-a-new-kind-of-thermostable-trehalose-synthase-from-thermobaculum-terrenum
#6
Junqing Wang, Xudong Ren, Ruiming Wang, Jing Su, Feng Wang
Trehalose has important applications in the food industry and pharmaceutical manufacturing. The thermostable enzyme trehalose synthase from Thermobaculum terrenum (TtTS) catalyzes the reversible interconversion of maltose and trehalose. Here, we investigated the structural characteristics of TtTS in complex with the inhibitor TriS. TtTS exhibits the typical three domain glycoside hydrolase family 13 structure. The catalytic cleft consists of Asp202-Glu244-Asp310 and various conserved substrate-binding residues...
August 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28806570/chiral-separation-of-terbutaline-and-non-steroidal-anti-inflammatory-drugs-by-using-a-new-lysine-bridged-hemispherodextrin-in-capillary-electrophoresis
#7
V Cucinotta, M Messina, A Contino, G Maccarrone, S Orlandini, A Giuffrida
A method for the separation of a mixture of terbutaline and non-steroidal anti-inflammatory drugs was developed using capillary electrophoresis with a new hemispherodextrin, ad hoc designed, the lysine - bridged hemispherodextrin (THLYSH). The use of lysine residues to bridge the trehalose capping unit moiety to the cyclodextrin cavity gives rise to a receptor with two long chains with amine nitrogen atoms, whose charge can be easily tuned as a function of the solution pH. The new hemispherodextrin was accurately characterised by ESI-MS and NMR spectroscopy, also highlighting its protonation behaviour...
August 3, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28806423/new-synthetic-lipid-antigens-for-rapid-serological-diagnosis-of-tuberculosis
#8
Alison Jones, Mark Pitts, Juma'a R Al Dulayymi, James Gibbons, Andrew Ramsay, Delia Goletti, Christopher D Gwenin, Mark S Baird
BACKGROUND: During pulmonary tuberculosis (PTB) antibodies are generated to trehalose esters of mycolic acids which are cell wall lipids of Mycobacterium tuberculosis (Mtb). Attempts have been made to use these complex natural mixtures in serological tests for PTB diagnosis. AIM: The aim of this work was to determine whether a serological test based on a panel of defined individual trehalose esters of characteristic synthetic mycolic acids has improved diagnostic accuracy in distinguishing patients with culture positive PTB from individuals who were Mtb culture negative...
2017: PloS One
https://www.readbyqxmd.com/read/28805713/polyaspartamide-based-nanoparticles-loaded-with-fluticasone-propionate-and-the-in-vitro-evaluation-towards-cigarette-smoke-effects
#9
Emanuela Fabiola Craparo, Maria Ferraro, Elisabetta Pace, Maria Luisa Bondì, Gaetano Giammona, Gennara Cavallaro
This paper describes the evaluation of polymeric nanoparticles (NPs) as a potential carrier for lung administration of fluticasone propionate (FP). The chosen polymeric material to produce NPs was a copolymer based on α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) whose backbone was derivatised with different molecules, such as poly(lactic acid) (PLA) and polyethylenglycol (PEG). The chosen method to produce NPs from PHEA-PLA-PEG2000 was the method based on high-pressure homogenization and subsequent solvent evaporation by adding Pluronic F68 during the process and trehalose before lyophilisation...
August 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28803845/improvement-of-post-thaw-sperm-survivals-using-liquid-nitrogen-vapor-in-a-spermcasting-oyster-ostrea-angasi
#10
Md Mahbubul Hassan, Xiaoxu Li, Jian G Qin
Low survival of cryopreserved sperm impedes the application of cryopreservation technique in spermcasting oyster species. This study developed a simple method of liquid nitrogen vapor freezing to improve post-thaw sperm survival in the spermcasting oyster Ostrea angasi. The results indicate that the permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG) were non-toxic to sperm up to 20% concentration and 90 min exposure whereas methanol at 10% or higher was toxic to sperm for any exposure over 30 min...
August 10, 2017: Cryobiology
https://www.readbyqxmd.com/read/28791019/macrophage-inducible-c-type-lectin-as-a-multifunctional-player-in-immunity
#11
REVIEW
Emmanuel C Patin, Selinda Jane Orr, Ulrich E Schaible
The macrophage-inducible C-type lectin (Mincle) is an innate immune receptor on myeloid cells sensing diverse entities including pathogens and damaged cells. Mincle was first described as a receptor for the mycobacterial cell wall glycolipid, trehalose-6,6'-dimycolate, or cord factor, and the mammalian necrotic cell-derived alarmin histone deacetylase complex unit Sin3-associated protein 130. Upon engagement by its ligands, Mincle induces secretion of innate cytokines and other immune mediators modulating inflammation and immunity...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28790995/trehalose-polyphleates-external-cell-wall-lipids-in-mycobacterium-abscessus-are-associated-with-the-formation-of-clumps-with-cording-morphology-which-have-been-associated-with-virulence
#12
Marta Llorens-Fons, Míriam Pérez-Trujillo, Esther Julián, Cecilia Brambilla, Fernando Alcaide, Thomas F Byrd, Marina Luquin
Mycobacterium abscessus is a reemerging pathogen that causes pulmonary diseases similar to tuberculosis, which is caused by Mycobacterium tuberculosis. When grown in agar medium, M. abscessus strains generate rough (R) or smooth colonies (S). R morphotypes are more virulent than S morphotypes. In searching for the virulence factors responsible for this difference, R morphotypes have been found to form large aggregates (clumps) that, after being phagocytozed, result in macrophage death. Furthermore, the aggregates released to the extracellular space by damaged macrophages grow, forming unphagocytosable structures that resemble cords...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28782503/cryopreservation-of-rat-hepatocytes-with-disaccharides-for-cell-therapy
#13
Liana Monteiro da Fonseca Cardoso, Marcelo Alves Pinto, Andrea Henriques Pons, Luiz Anastácio Alves
Cryopreservation of hepatocytes is a crucial step in the implementation of cell therapy for treating certain liver diseases. In the present study we investigated the effect of the some disaccharides on the cryopreservation of rat hepatocytes. Liver cells were frozen in media in the presence or absence of low concentrations of Me2SO (5% Me2SO) supplemented with varying concentrations of disaccharides (sucrose, glucose and trehalose). After 7 days of cryopreservation, the hepatocytes were thawed and viability was measure by exclusion of trypan blue and by the MTT technique, as well as by determining albumin production...
August 4, 2017: Cryobiology
https://www.readbyqxmd.com/read/28780275/inhalable-sirna-loaded-nano-embedded-microparticles-engineered-using-microfluidics-and-spray-drying
#14
Monica Agnoletti, Adam Bohr, Kaushik Thanki, Feng Wan, Xianghui Zeng, Johan Peter Boetker, Mingshi Yang, Camilla Foged
Medicines based on small interfering RNA (siRNA) are promising for the treatment of a number of lung diseases. However, efficient delivery systems and design of stable dosage forms are required for inhalation therapy, as well as cost-effective methods for manufacturing of the final product. In this study, a 3D-printed micromixer was used for preparation of siRNA-dendrimer nanocomplexes, which were subsequently processed into microparticle-based dry powders for inhalation using spray drying. By applying the disposable micromixer, nanocomplexes were prepared of an average hydrodynamic diameter comparable to that of nanocomplexes prepared by manual mixing, but with narrower size distribution and low batch-to-batch variation...
August 2, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28776690/application-of-biopolymers-for-improving-the-glass-transition-temperature-of-hairtail-fish-meat
#15
Haixia Yu, Shuibing Yang, Chunhong Yuan, Qinglan Hu, Yuan Li, Shiguo Chen, Yaqin Hu
BACKGROUND: Tg and food moisture content is closely related especially in high moisture content such as surimi products. In order to improve the storage condition and keep food quality, the influence of six biopolymers on the Tg of hairtail fish meat paste was investigated by differential scanning colorimetry. RESULTS: Obtained samples were stored at -8 °C (>Tg), -14 °C (Tg) and -18 °C (< Tg) for 105 days. The results showed that trehalose, maltodextrin and xanthan could obviously improve the Tg of the hairtail fish meat paste...
August 4, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28772113/structural-integrity-and-developmental-potential-of-spermatozoa-following-microwave-assisted-drying-in-the-domestic-cat-model
#16
Jennifer L Patrick, Gloria D Elliott, Pierre Comizzoli
Characterizing the resilience of mammalian cells to non-physiological conditions is necessary to develop preservation and long-term storage strategies at low or ambient temperatures. Using the domestic cat model, the objective of the study was to characterize structural integrity (morphology and DNA damage) as well as functional properties (sperm aster formation and embryo formation after sperm injection) of spermatozoa after microwave-assisted drying to a moisture content compatible with storage in a glassy state at supra-zero temperatures...
July 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28771200/dissecting-tissue-specific-transcriptomic-responses-from-leaf-and-roots-under-salt-stress-in-petunia-hybrida-mitchell
#17
Gonzalo H Villarino, Qiwen Hu, Michael J Scanlon, Lukas Mueller, Aureliano Bombarely, Neil S Mattson
One of the primary objectives of plant biotechnology is to increase resistance to abiotic stresses, such as salinity. Salinity is a major abiotic stress and increasing crop resistant to salt continues to the present day as a major challenge. Salt stress disturbs cellular environment leading to protein misfolding, affecting normal plant growth and causing agricultural losses worldwide. The advent of state-of-the-art technologies such as high throughput mRNA sequencing (RNA-seq) has revolutionized whole-transcriptome analysis by allowing, with high precision, to measure changes in gene expression...
August 3, 2017: Genes
https://www.readbyqxmd.com/read/28763971/impact-of-trehalose-on-the-activity-of-sodium-and-potassium-chloride-in-aqueous-solutions-why-trehalose-is-worth-its-salt
#18
Michal Poplinger, Ilan Shumilin, Daniel Harries
Trehalose is revered for its multiple unique impacts on solution properties, including the ability to modulate the salty and bitter tastes of sodium and potassium salts. However, the molecular mechanisms underlying trehalose's effect on taste perception are unknown. Here we focus on the physico-chemical effect of trehalose to alter the activity of monovalent salts in aqueous solution. Using a modified isopiestic methodology that relies on contemporary vapor pressure osmometry, we elucidate how trehalose modifies the thermodynamic chemical activity of sodium and potassium chloride, as well as the effect of the salts on the sugar's activity...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28758834/preferential-interactions-of-trehalose-l-arginine-hcl-and-sodium-chloride-with-therapeutically-relevant-igg1-monoclonal-antibodies
#19
Chaitanya Sudrik, Theresa Cloutier, Phuong Pham, Hardeep S Samra, Bernhardt L Trout
Preferential interactions of weakly interacting formulation excipients govern their effect on the equilibrium and kinetics of several reactions of protein molecules in solution. Using vapor pressure osmometry, we characterized the preferential interactions of commonly used excipients trehalose, L-arginine.HCl and NaCl with three therapeutically-relevant, IgG1 monoclonal antibodies that have similar size and shape, but differ in their surface hydrophobicity and net charge. We further characterized the effect of these excipients on the reversible self-association, aggregation and viscosity behavior of these antibody molecules...
July 31, 2017: MAbs
https://www.readbyqxmd.com/read/28755694/better-cold-tolerance-of-bt-resistant-spodoptera-exigua-strain-and-the-corresponding-cold-tolerant-mechanism
#20
Honghua Su, Jincheng Zou, Qiuxia Zhou, Qi Yu, Yong Yang, Yizhong Yang
Spodoptera exigua is a secondary target pest of Bt cotton commercialized in China. With the continuous adoption of Bt cotton, populations of S. exigua have gradually increased. However, the cold tolerance ability of Bt-resistant S. exigua and the effect of continuous Bt diet on anti-cold materials are unknown. In our study, it was found that Bt-resistant S. exigua (Bt10) developed better with shorter larval and pupal duration and higher pupation rate compared to CK at the suboptimal low temperature. The supercooling points and freezing points of the Bt-resistant S...
August 2017: Pesticide Biochemistry and Physiology
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