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Fakhrossadat Emami, Alireza Vatanara, Abdolhosein Rouholamini Najafabadi, Yejin Kim, Eun Ji Park, Soroush Sardari, Dong Hee Na
The purpose of this study was to prepare spray freeze-dried particles of immunoglobulin G (IgG) using various combinations of trehalose and different amino acids (leucine, phenylalanine, arginine, cysteine, and glycine), and investigate the effect of the amino acids on the stability of IgG during the spray freeze-drying (SFD) process and storage. The morphology and structural integrity of the processed nanoparticles were evaluated by physical and spectroscopic techniques. SFD-processed IgG without any excipient resulted in the formation of aggregates corresponding to approximately 14% of IgG...
April 9, 2018: European Journal of Pharmaceutical Sciences
Nikhil Gupta, Jayakanthan Kabeerdoss, Hindhumathi Mohan, Ruchika Goel, Debashish Danda
Introduction: Suspicion on the association between Takayasu Arteritis (TA) and Tubcerculosis (TB) has been in vogue for years. Prevalence of TB in TA is reported to be higher. We aimed to study innate immune responses in patients with TA on exposure to Trehalose-6,6-dibehenate (TDB), a synthetic analogue of Trehalose-6,6-Dimycolate (TDM, also known as mycobacterial cord factor) in comparison with healthy controls. Materials and Methods: Patients with type V TA, satisfying 1990 ACR criteria, and age and sex matched healthy controls were recruited...
2018: Open Rheumatology Journal
Seyed Masoud Jafarpour, Mehdi Safaei, Mehran Mohseni, Morteza Salimian, Akbar Aliasgharzadeh, Bagher Fahood
Background: Thyroid cancer has been growing rapidly during the last decades. Radioiodine-131 (I-131) as an appropriate therapy modality is currently using in the treatment of cancer and hyperthyroidism diseases. This radiotracer is considered as a cause of oxidative DNA damage in nontarget cells and tissues. The aim of this study was to investigate the effects of curcumin and trehalose on the level of DNA double-strand breaks (DSBs) caused by I-131 in human lymphocytes. Materials and Methods: First, 6-mL blood samples were taken from each of the five volunteers...
April 2018: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
Emad Ibrahim, Pavel Dobeš, Martin Kunc, Pavel Hyršl, Dalibor Kodrík
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1 , AdoR1 (adenosine receptor), and Akh1 AdoR1 . Mortality decreased in all mutants post-EPN infection compared with the control (w1118 ). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism...
April 5, 2018: Journal of Insect Physiology
V Carli, L Menu-Bouaouiche, P Cardinael, L Benissan, G Coquerel
OBJECTIVE: The objective of this work is to show the feasibility of manufacturing from a spray drying process particles containing immunoglobulin G capable of being administered by inhalation via a pressurized metered dose inhaler. METHODS: Spray drying were made from aqueous solutions containing IgG and two types of excipients, mannitol and trehalose, with two ratios: 25% w/w and 75%w/w. The physicochemical and aerodynamic properties of the powders obtained were characterized just after manufacturing and after 1 month of storage at 40°C/75% RH according to criteria defined as needed to satisfy an inhaled formulation with a pressurized metered dose inhaler...
April 4, 2018: Annales Pharmaceutiques Françaises
Meng Zhao, Xiangqun Xu, Shaolong Yang, Tianming Liu, Bo Liu
The maltooligosyl trehalose synthase gene from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7 was cloned and the recombinant peotein was expressed in E. coli. The protein was purified to homogeneity by nickel column chromatography. The archaeal enzyme could catalyze an intramolecular transglycosylation reaction and convert the glycosidic bond at the reducing end of dextrins from α-1, 4 (reducing end) into α-1, 1 (non-reducing end). The most suitable temperature was 75°C and the optimal pH was 5...
March 2018: Pakistan Journal of Pharmaceutical Sciences
Hong Jiang, Si-Jia Xue, Yan-Feng Li, Guang-Lei Liu, Zhen-Ming Chi, Zhong Hu, Zhe Chi
A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor...
August 15, 2018: Food Chemistry
Dharmesh Gol, Shreya Thakkar, Manju Misra
OBJECTIVE: In present work nanocrystal based formulation of Risperidone (RIS) was proposed to overcome solubility issue of RIS, while lyophilization technique was used effectively, for conversion of RIS nanosuspension to solid state. SIGNIFICANCE: RIS nanosuspension was developed and stabilized with a combination of Polycaprolactone and Pluronic® F-68 as stabilizers. With focus on critical parameters like nature of cryoprotectants and effect of eutectic temperature on properties of nanosuspension, the suitability of lyophilization technique in improving the physical stability of prepared nanosuspension was also evaluated...
April 5, 2018: Drug Development and Industrial Pharmacy
Chao Zhang, Xi Peng, Xiaofeng Guo, Gaijuan Tang, Fengli Sun, Shudong Liu, Yajun Xi
Background: Switchgrass ( Panicum virgatum L.) is a model biofuel plant because of its high biomass, cellulose-richness, easy degradation to ethanol, and the availability of extensive genomic information. However, a little is currently known about the molecular responses of switchgrass plants to dehydration stress, especially multiple dehydration stresses. Results: Studies on the transcriptional profiles of 35-day-old tissue culture plants revealed 741 dehydration memory genes...
2018: Biotechnology for Biofuels
Xuewei Yang, Ni Ma, Le Yang, Yaqing Zheng, Zhengyi Zhen, Qing Li, Meihua Xie, Juan Li, Ke-Qin Zhang, Jinkui Yang
Rab GTPases are the largest group of the small GTPases family, which play a pivotal role in the secretion of proteins. Arthrobotrys oligospora is a representative nematode-trapping fungus that can produce adhesive networks to capture nematodes. In this study, the roles of two Rab GTPases AoRab-7A and AoRab-2 were characterized by gene knockout in the fungus A. oligospora. The disruption of AoRab-7A hindered the mycelial growth in different media, the conidiation of ΔAoRab-7A transformants was almost abolished, and the transcription of four sporulation-related genes (AbaA, FluG, Hyp1, and VosA) was downregulated compared to the wild-type strain (WT)...
April 3, 2018: Applied Microbiology and Biotechnology
Jagdish Gopal Paithankar, Shamprasad Varija Raghu, Rajashekhar K Patil
PURPOSE: During development, various life stages of Drosophila melanogaster (D. melanogaster) show different levels of resistance to gamma irradiation, with the early pupal stage being the most radiation sensitive. This provides us an opportunity to explore the biochemical basis of such variations. The present study was carried out to understand the mechanisms underlying radiation resistance during life stages of D. melanogaster. MATERIALS AND METHODS: Homogenates from all the life stages of D...
April 3, 2018: International Journal of Radiation Biology
Esther Gamero-Sandemetrio, Lucía Payá-Tormo, Rocío Gómez-Pastor, Agustín Aranda, Emilia Matallana
Several yeast species, belonging to Saccharomyces and non- Saccharomyces genera, play fundamental roles during spontaneous must grape fermentation, and recent studies have shown that mixed fermentations, co-inoculated with S. cerevisiae and non- Saccharomyces strains, can improve wine organoleptic properties. During active dry yeast (ADY) production, antioxidant systems play an essential role in yeast survival and vitality as both biomass propagation and dehydration cause cellular oxidative stress and negatively affect technological performance...
January 26, 2018: Microbial Cell
Ying-Ying Sun, Wen-Jing Zhou, Hui Wang, Gan-Lin Guo, Zhen-Xia Su, Yin-Fang Pu
Nine antialgal active compounds, (i.e. trehalose (1), twenty-two methyl carbonate (2), (-)-dihydromenisdaurilide (3), 3,7,11,15-tetramethyl-2-hexadecen-1-ol (4), isophytol (5), 8-hexadecenol (6), 17-hydroxyheptadecanoic acid (7), trans-asarone (8) and 2-amino-3-mercaptopropanoic acid (9)) were isolated from Ulva pertusa for the first time by sephadex LH-20 column chromatography, silica gel column chromatography and repeated preparative TLC. Except for compound 4, all compounds represented novel isolated molecules from marine macroalgae...
March 29, 2018: Ecotoxicology and Environmental Safety
HongLing Liu, ShaoJie Yang, Qiang Liu, Ruiming Wang, Tengfei Wang
The process for production of trehalose using trehalose synthase (TreS) to convert maltose into trehalose in one step is highly desirable in the industry. Nonetheless, the studies on industrial-scale production of trehalose by recombinant TreS in Escherichia coli are still scarce. In this study, a TreS from Pseudomonas putida ATCC47054 was expressed in E. coli BL21(DE3) via plasmids pET15b and pET22b. pET15b-treS showed better plasmid stability and TreS expression, which revealed that the highest activity, 39866±1420U/(g dry cell weight) at the final lactose concentration of 4g/L for 7h at 27°C in a 5-L fermentor at pH 8...
June 2018: Enzyme and Microbial Technology
Nidhi Katyal, Manish Agarwal, Raktim Sen, Vinay Kumar, Shashank Deep
Aggregation of α-synuclein is closely connected to the pathology of Parkinson's disease. The phenomenon involves multiple steps, commenced by partial misfolding and eventually leading to mature amyloid fibril formation. Trehalose, a widely accepted osmolyte, has been shown previously to inhibit aggregation of various globular proteins owing to its ability to prevent the initial unfolding of protein. In this study, we have examined if it behaves in a similar fashion with intrinsically disordered protein α-synuclein and possesses the potential to act as therapeutic agent against Parkinson's disease...
March 30, 2018: ACS Chemical Neuroscience
Christian A Söldner, Anselm H C Horn, Heinrich Sticht
Synthetic analogues of mycobacterial trehalose-dimycolate such as trehalose acyl esters have been proposed as novel adjuvants for vaccination. They induce an immune response by binding to the macrophage C-type lectin receptor Mincle. The binding site of trehalose is known, but there is yet only very limited structural information about the binding mode of the acyl esters. Here, we performed a systematic molecular dynamics study of trehalose mono-and diesters with different chain lengths. All acyl chains investigated exhibited a high flexibility and interacted almost exclusively with a hydrophobic groove on Mincle...
March 29, 2018: Scientific Reports
Juan M Hernández-Meza, José G Sampedro
Lactate dehydrogenase (LDH) catalyzes the reduction of pyruvate to lactate by using NADH. LDH kinetics has been proposed to be dependent on the dynamics of a loop over the active site. Kramers´ theory has been useful in the study of enzyme catalysis dependent on large structural dynamics. In this work, LDH kinetics was studied in the presence of trehalose and at different temperatures. In the absence of trehalose, temperature increase raised exponentially the LDH Vmax and revealed a sigmoid transition of Km towards a low-affinity state similar to protein unfolding...
March 29, 2018: Journal of Physical Chemistry. B
Andrey A Kuzhelev, Olesya A Krumkacheva, Georgiy Yu Shevelev, Maxim Yulikov, Matvey V Fedin, Elena G Bagryanskaya
Electron paramagnetic resonance (EPR) based nanometer distance measurements at ambient temperatures are of particular interest for structural biology applications. The nitroxide spin labels commonly used in EPR reveal relatively short transverse relaxation under these conditions, which limits their use for detecting static dipolar interactions. At the same time, the longitudinal relaxation of nitroxide spin labels is still long enough to allow using them as 'pumped' species in the relaxation induced dipolar modulation enhancement (RIDME) experiment where the detection is carried out on the slower relaxing triarylmethyl (TAM) spin labels...
March 29, 2018: Physical Chemistry Chemical Physics: PCCP
Matthew J Paul, Asier Gonzalez-Uriarte, Cara A Griffiths, Keywan Hassani-Pak
No abstract text is available yet for this article.
March 28, 2018: Plant Physiology
Lukas Boge, Amanda Västberg, Anita Umerska, Helena Bysell, Jonny Eriksson, Katarina Edwards, Anna Millqvist-Fureby, Martin Andersson
Liquid crystalline nanoparticles (LCNPs), e.g. cubosomes and hexosomes, are receiving more and more attraction as drug delivery vehicles. Dry powder formulation that forms LCNPs upon hydration can be advantageous to make new routes of administration accessible. In this work, we investigate use of three disaccharides (lactose, trehalose and sucrose) as protective matrices for glycerol monooleate based LCNP forming powders produced by freeze-drying. Phase behavior, particle size and size distributions at the different preparation steps were monitored by small angle x-ray scattering (SAXS) and dynamic light scattering (DLS)...
March 20, 2018: Journal of Colloid and Interface Science
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