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https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#1
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28076661/impression-techniques-used-for-single-unit-crowns-findings-from-the-national-dental-practice-based-research-network
#2
Michael S McCracken, David R Louis, Mark S Litaker, Helena M Minyé, Thomas Oates, Valeria V Gordan, Don G Marshall, Cyril Meyerowitz, Gregg H Gilbert
PURPOSE: To: (1) determine which impression and gingival displacement techniques practitioners use for single-unit crowns on natural teeth; and (2) test whether certain dentist and practice characteristics are significantly associated with the use of these techniques. MATERIALS AND METHODS: Dentists participating in the National Dental Practice-Based Research Network were eligible for this survey study. The study used a questionnaire developed by clinicians, statisticians, laboratory technicians, and survey experts...
January 11, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28059049/evaluation-of-the-3m%C3%A2-molecular-detection-assay-mda-2-listeria-monocytogenes-for-the-detection-of-listeria-monocytogenes-in-select-foods-and-environmental-surfaces-collaborative-study-first-action-2016-08
#3
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Lisa Monteroso
The 3M<sup>™</sup> Molecular Detection Assay (MDA) 2 <em>- Listeria </em>uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect <em>Listeria </em>spp. in a broad range of food types and environmental surfaces. Using an unpaired study design, the MDA 2 - <em>Listeria </em>was compared to the U.S. Department of Agriculture Food Safety and Inspection Service <em>Microbiology Laboratory Guidebook </em>Chapter 8.09 "<em>Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products, and Environmental Samples</em>" reference method for the detection of <em>Listeria </em>in deli turkey and raw chicken breast fillet...
November 17, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28050388/natural-alternatives-for-chemicals-used-in-histopathology-lab-a-literature-review
#4
REVIEW
Ananthalakshmi Ramamoorthy, Shivani Ravi, Nadeem Jeddy, Radhika Thangavelu, Sunitha Janardhanan
Histopathology lab is the place where the specimen gets processed and stained to view under microscope for interpretation. Exposure to the chemicals used in these processes cause various health hazards to the laboratory technicians, pathologists, and scientists working in the laboratory. Hence, there is a dire need to introduce healthy and bio-friendly alternatives in the field. This literature review explores the natural products and their efficiency to be used as alternatives for chemicals in the histopathology lab...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28024819/randomized-controlled-within-subject-evaluation-of-digital-and-conventional-workflows-for-the-fabrication-of-lithium-disilicate-single-crowns-part-ii-cad-cam-versus-conventional-laboratory%C3%A2-procedures
#5
Irena Sailer, Goran I Benic, Vincent Fehmer, Christoph H F Hämmerle, Sven Mühlemann
STATEMENT OF PROBLEM: Clinical studies are needed to evaluate the entire digital and conventional workflows in prosthetic dentistry. PURPOSE: The purpose of the second part of this clinical study was to compare the laboratory production time for tooth-supported single crowns made with 4 different digital workflows and 1 conventional workflow and to compare these crowns clinically. MATERIAL AND METHODS: For each of 10 participants, a monolithic crown was fabricated in lithium disilicate-reinforced glass ceramic (IPS e...
December 23, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#6
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
December 23, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/28011231/metabolic-syndrome-and-occupation-any-association-prevalence-among-auto-technicians-and-school-teachers-in-south-west-nigeria
#7
Adeseye A Akintunde, Taiwo W Oloyede
AIMS: To determine occupational association with metabolic syndrome among auto technicians and school teachers. METHODS: One hundred and sixty six subjects were selected for this study. Clinical data was obtained while laboratory investigations including plasma glucose and lipid profile were performed. Statistical analysis was done with SPSS 20.0. P<0.05 was taken as statistically significant. RESULTS: School teachers had significantly higher mean total cholesterol and LDL-cholesterol compared to auto technicians...
December 14, 2016: Diabetes & Metabolic Syndrome
https://www.readbyqxmd.com/read/27998378/validation-of-contact-free-sleep-monitoring-device-with-comparison-to-polysomnography
#8
Asher Tal, Zvika Shinar, David Shaki, Shlomi Codish, Aviv Goldbart
STUDY OBJECTIVES: To validate a contact-free system designed to achieve maximal comfort during long-term sleep monitoring, together with high monitoring accuracy. METHODS: We used a contact-free monitoring system (EarlySense, Ltd., Israel), comprising an under-the-mattress piezoelectric sensor and a smartphone application, to collect vital signs and analyze sleep. Heart rate (HR), respiratory rate (RR), body movement, and calculated sleep-related parameters from the EarlySense (ES) sensor were compared to data simultaneously generated by the gold standard, polysomnography (PSG)...
December 16, 2016: Journal of Clinical Sleep Medicine: JCSM: Official Publication of the American Academy of Sleep Medicine
https://www.readbyqxmd.com/read/27977585/neurocognitive-performance-and-physical-function-do-not-change-with-physical-cognitive-mindfulness-training-in-female-laboratory-technicians-with-chronic-musculoskeletal-pain-randomized-controlled-trial
#9
Kenneth Jay, Mikkel Brandt, Mc Schraefel, Markus Due Jakobsen, Emil Sundstrup, Gisela Sjøgaard, Jonas Vinstrup, Lars L Andersen
BACKGROUND: Cognitive and physical performance can be negatively affected by chronic pain. This study evaluates the effect of combined physical-, cognitive-, and mindfulness training (PCMT) on cognitive and physical performance. METHODS: From a large pharmaceutical company in Denmark we randomly allocated 112 female laboratory technicians with chronic upper limb pain to group-based PCMT at the worksite or a reference group for 10 weeks. Neurocognitive performance was measured by the computerized central nervous system vital signs neurocognitive assessment battery...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27960550/ensuring-the-collection-of-high-quality-dried-blood-spot-samples-across-multisite-clinical-studies
#10
Tina Panchal, Neil Spooner, Matthew Barfield
AIM: The quality of quantitative analytical measurements is dependent on the quality of the sample collected, and dried blood spots (DBS) are no exception. As the use of DBS has matured into late-stage clinical drug-development studies, it has become apparent that a simple and straightforward approach in a controlled single-site, first-time-into-human clinic, does not always translate into multicenter clinical studies. Using synthetic blood, a method of training and assessing clinical laboratory staff has been developed to ensure the quality of sampling...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27931315/factors-affecting-the-vocational-calling-of-laboratory-animal-care-and-research-employees
#11
Gregory P Boivin, Ronald J Markert
We surveyed laboratory animal care and research workers to determine the factors affecting their vocational calling. The survey comprised 56 questions in 4 groups: passion, job stability or happiness, work volition, and demographics. We hypothesized that personnel who worked in the field a longer time, were older, had higher education levels, were involved with AALAS, and in higher positions in their organization were more likely to indicate a calling to the laboratory animal care field. In addition, we hypothesized that job satisfaction and classifying one's job as a calling were positively related to organizational support and work volition...
November 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/27906464/methodological-considerations-for-optimizing-and-validating-behavioral-assays
#12
Stacey J Sukoff Rizzo, Jill L Silverman
Preclinical animal models are indispensable tools for translational research for which behavioral characterization and phenotyping are essential to testing hypotheses and for evaluating the potential of novel therapeutic agents to treat diseases. The methods employed for comprehensive behavioral phenotyping and pharmacological experiments are complex and should be conducted exclusively by trained technicians with demonstrated proficiency. The ultimate goal is to identify disease-relevant and translational behavioral endpoints that are robust, reliable, and reproducible, and that can be employed to evaluate potential of novel therapeutic agents to treat disease...
December 1, 2016: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/27904162/impact-of-catheterization-lab-computer-software-settings-on-hemodynamic-assessment-of-aortic-stenosis
#13
Zaher Fanari, Anitha Rajamanickam, Mathew Grove, Sumaya Hammami, Cassie Walls, Paul Kolm, William Weintraub, Andrew J Doorey
BACKGROUND: Accurate assessment of Cardiac Output (CO) is a critical measurement in the calculation of aortic valve area (AVA). Due to the known inaccuracy of estimated Fick calculations, many measure thermodilution (TD) CO as well due to previous studies showing better correlation with the gold standard direct CO. Previous studies showed suboptimal correlation between both methods. Most physicians assume that the TD CO is chosen by catheterization laboratory software for AVA evaluation...
July 2016: Delaware Medical Journal
https://www.readbyqxmd.com/read/27891473/a-comparison-of-conventional-and-microwave-decalcification-and-processing-of-tooth-and-mandibular-bone-specimens
#14
A Thirumal Raj, Shankargouda Patil, Roopa S Rao
INTRODUCTION: Any laboratory procedure exposes the technician and the pathologists to the hazardous effects of chemicals. Conventional procedures like decalcification and histo-processing employed in laboratories are labour intense and time consuming thereby delaying the report dispatch. The present study was an attempt to employ a kitchen microwave to hasten the process and facilitate faster and accurate reporting; thus, benefitting the technician, pathologist and the patient. AIM: To compare conventional and microwave based decalci-fication, processing and staining of tooth and mandibular bone specimens using 5% nitric acid as decalcifying agent...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27885097/results-of-relid-study-2014-buenos-aires-argentina-retrospective-evaluation-of-lens-injuries-and-dose
#15
C Papp, M Romano-Miller, A Descalzo, S Michelin, A Molinari, A Rossini, C Plotkin, G Bodino, G Esperanza, M Di Giorgio, R Touzet
High levels of scatter radiation in catheterization laboratories may lead to posterior subcapsular opacities in the lens of the staff. The international Retrospective Evaluation of Lens Injuries and Dose (RELID) was performed in Argentina for the first time in 2010 in the context of the congress of the Latin American Society of Interventional Cardiology (SOLACI) and recently, in 2014, was carried out for the second time (SOLACI-CACI 2014). The 2014 study included 115 participants: interventional cardiologists, technicians and nurses...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27884215/public-perception-of-emergency-medical-services-in-the-united-states
#16
Remle P Crowe, Roger Levine, Severo Rodriguez, Ashley D Larrimore, Ronald G Pirrallo
OBJECTIVE: The objective of this study was to assess the public's experience, expectations, and perceptions related to Emergency Medical Services (EMS). METHODS: A population-based telephone interview of adults in the United States was conducted. The survey instrument consisted of 112 items. Demographic variables including age, race, political beliefs, and household income were collected. Data collection was performed by trained interviewers from Kent State University's (Kent, Ohio USA)Social Research Laboratory...
December 2016: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/27872822/automating-hesc-differentiation-with-3d-printing-and-legacy-liquid-handling-solutions
#17
Eric Zluhan, Kathleen Kelly, Nick LeClair, Danique Wortel, Kesley Moody
Historically, the routine use of laboratory automation solutions has been prohibitively expensive for many laboratories. As legacy hardware has begun to emerge on the secondary market, automation is becoming an increasingly affordable option to augment workflow in virtually any laboratory. To assess the utility of legacy liquid handling in stem cell differentiation, a used liquid handling robot was purchased at auction to automate a stem cell differentiation protocol that gives rise to CD14 + CD45+ mononuclear cells...
2016: MethodsX
https://www.readbyqxmd.com/read/27870167/design-of-complete-dentures-by-adopting-cad-developed-for-fixed-prostheses
#18
Yanfeng Li, Weili Han, Jing Cao, Yuan Iv, Yue Zhang, Yishi Han, Yi Shen, Zheng Ma, Huanyue Liu
The demand for complete dentures is expected to increase worldwide, but complete dentures are mainly designed and fabricated manually involving a broad series of clinical and laboratory procedures. Therefore, the quality of complete dentures largely depends on the skills of the dentist and technician, leading to difficulty in quality control. Computer-aided design and manufacturing (CAD/CAM) has been used to design and fabricate various dental restorations including dental inlays, veneers, crowns, partial crowns, and fixed partial dentures (FPDs)...
November 21, 2016: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/27848921/staphylococcus-aureus-with-panton-valentine-toxin-skin-infection-in-a-medical-laboratory-technician
#19
Richard Pougnet, Laurence Pougnet
This report exposes the case of a Staphylococcus aureus infection occurring in a microbiology laboratory technician. He was a 52 year-old man without medical history. He presented an abscess on the anterior aspect of the left forearm. Analysis showed that it was a Staphylococcus aureus secreting the Panton-Valentine toxin. The study of the workplace found the frequency of exposure. The study of workstation showed the link between the technician position and the infection. Indeed, this man touched an area where the biocleaning was hard to do...
December 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27843164/implementation-research-a-mentoring-programme-to-improve-laboratory-quality-in-cambodia
#20
Lucy A Perrone, Vireak Voeurng, Sophat Sek, Sophanna Song, Nora Vong, Chansamrach Tous, Jean-Frederic Flandin, Deborah Confer, Alexandre Costa, Robert Martin
OBJECTIVE: To implement a mentored laboratory quality stepwise implementation (LQSI) programme to strengthen the quality and capacity of Cambodian hospital laboratories. METHODS: We recruited four laboratory technicians to be mentors and trained them in mentoring skills, laboratory quality management practices and international standard organization (ISO) 15189 requirements for medical laboratories. Separately, we trained staff from 12 referral hospital laboratories in laboratory quality management systems followed by tri-weekly in-person mentoring on quality management systems implementation using the LQSI tool, which is aligned with the ISO 15189 standard...
October 1, 2016: Bulletin of the World Health Organization
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