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https://www.readbyqxmd.com/read/28214162/knowledge-attitude-and-practice-of-blood-culture-contamination-a-multicenter-study
#1
Archana Nair, Sean P Elliott, Mayar Al Mohajer
We developed a questionnaire to assess the knowledge and practice of nursing staff, patient care assistants, phlebotomists, and laboratory technicians regarding blood culture collection and contamination at 2 tertiary care academic centers. Our study showed there were several gaps in knowledge and practice regarding obtaining blood cultures.
February 15, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28212541/analysis-of-variability-in-high-throughput-screening-data-applications-to-melanoma-cell-lines-and-drug-responses
#2
Kuan-Fu Ding, Darren Finlay, Hongwei Yin, William P D Hendricks, Chris Sereduk, Jeffrey Kiefer, Aleksandar Sekulic, Patricia M LoRusso, Kristiina Vuori, Jeffrey M Trent, Nicholas J Schork
High-throughput screening (HTS) strategies and protocols have undergone significant development in the last decade. It is now possible to screen hundreds of thousands of compounds, each exploring multiple biological phenotypes and parameters, against various cell lines or model systems in a single setting. However, given the vast amount of data such studies generate, the fact that they use multiple reagents, and are often technician-intensive, questions have been raised about the variability, reliability and reproducibility of HTS results...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28203010/-evaluation-of-production-and-clinical-working-time-of-computer-aided-design-computer-aided-manufacturing-cad-cam-custom-trays-for-complete-denture
#3
L Wei, H Chen, Y S Zhou, Y C Sun, S X Pan
OBJECTIVE: To compare the technician fabrication time and clinical working time of custom trays fabricated using two different methods, the three-dimensional printing custom trays and the conventional custom trays, and to prove the feasibility of the computer-aided design/computer-aided manufacturing (CAD/CAM) custom trays in clinical use from the perspective of clinical time cost. METHODS: Twenty edentulous patients were recruited into this study, which was prospective, single blind, randomized self-control clinical trials...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28202894/prevalence-of-tuberculosis-among-health-care-workers-in-tuberculosis-specialized-hospitals-in-china
#4
Jian Du, Yu Pang, Yan Ma, Fengling Mi, Yuhong Liu, Liang Li
OBJECTIVES: Tuberculosis (TB) specialized hospitals are playing an increasingly important role in the diagnosis and treatment of patients with TB in China. This study aimed to investigate the prevalence of TB among health care workers (HCWs) in TB specialized hospitals in China. METHODS: We conducted a cross-sectional survey to collect longitudinal information on the number of HCWs and patients with TB from HCWs among 203 TB hospitals in China. RESULTS: Overall, these 186 TB cases accounted for the incidence of 985 per 100,000 population...
February 15, 2017: Journal of Occupational Health
https://www.readbyqxmd.com/read/28129324/performance-of-facspresto-point-of-care-instrument-for-cd4-t-cell-enumeration-in-human-immunodeficiency-virus-hiv-infected-patients-attending-care-and-treatment-clinics-in-belgium-and-tanzania
#5
Géraldine Daneau, Said Aboud, Irena Prat, Willy Urassa, Luc Kestens
BACKGROUND: CD4 T-cell counts are widely used to assess treatment eligibility and to follow-up HIV-infected patients. The World Health Organization prequalification of in vitro diagnostics program conducted a performance evaluation of the FACSPresto (BD Biosciences), a new point-of-care instrument to measure absolute CD4-T cell (CD4) counts and percentages in venous and capillary blood samples from HIV-infected patients. METHODS: Patients were recruited in Belgium (200 patients) and in Tanzania (247 patients)...
2017: PloS One
https://www.readbyqxmd.com/read/28124964/-investigation-of-the-prevalence-of-trichomonas-vaginalis-among-female-syrian-refugees-with-the-complaints-of-vaginitis-aged-between-15-49-years
#6
Nebiye Yentür Doni, Mustafa Aksoy, Zeynep Şimşek, Gülcan Gürses, Neşe Gül Hilali, Fadile Yıldız Zeyrek, Behire Özek, Gökhan Yıldırımkaya
Since the Syrian civil war began in 2011, most of the Syrian refugees have immigrated to Turkey due to its open gate policy and the width of the border. By the end of 2015, it was estimated that there were 2.5 million Syrian refugees in Turkey. Many of the Syrian refugees live in Sanliurfa due to its location on the border with Syria. Trichomonas vaginalis, apart from viral agents is the most common parasite among sexually transmitted infection agents. The aim of this study was to determine the prevalence of T...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28116567/-point-of-care-tests-for-the-rapid-diagnosis-of-shigellosis
#7
C Haddar, E Begaud, J Maslin, Y Germani
Worldwide, it is estimated that 140 million people suffer from shigellosis annually. The traditional identification of Shigella spp. by culture lacks sensitivity. Rapid diagnosis of shigellosis is important because it allows to engage appropriate antimicrobial treatment that shortens the duration and severity of the illness and reduces microbial carriage, thus the spread of infection in the community. Onestep immunochromatographic dipstick tests have been successfully developed at Institut Pasteur for Shigella spp...
January 23, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/28114988/performance-of-health-laboratories-in-provision-of-hiv-diagnostic-and-supportive-services-in-selected-districts-of-tanzania
#8
Deus S Ishengoma, Mathias L Kamugisha, Acleus S M Rutta, Gibson B Kagaruki, Andrew M Kilale, Amos Kahwa, Erasmus Kamugisha, Vito Baraka, Celine I Mandara, Godlisten S Materu, Julius J Massaga, Stephen M Magesa, Martha M Lemnge, Leonard E G Mboera
BACKGROUND: Roll-out and implementation of antiretroviral therapy (ART) necessitated many countries in Sub-Saharan Africa to strengthen their national health laboratory systems (NHLSs) to provide high quality HIV diagnostic and supportive services. This study was conducted to assess the performance of health laboratories in provision of HIV diagnostic and supportive services in eight districts (from four regions of Iringa, Mtwara, Tabora and Tanga), after nine years of implementation of HIV/AIDS care and treatment plan in Tanzania...
January 23, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#9
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28076661/impression-techniques-used-for-single-unit-crowns-findings-from-the-national-dental-practice-based-research-network
#10
Michael S McCracken, David R Louis, Mark S Litaker, Helena M Minyé, Thomas Oates, Valeria V Gordan, Don G Marshall, Cyril Meyerowitz, Gregg H Gilbert
PURPOSE: To: (1) determine which impression and gingival displacement techniques practitioners use for single-unit crowns on natural teeth; and (2) test whether certain dentist and practice characteristics are significantly associated with the use of these techniques. MATERIALS AND METHODS: Dentists participating in the National Dental Practice-Based Research Network were eligible for this survey study. The study used a questionnaire developed by clinicians, statisticians, laboratory technicians, and survey experts...
January 11, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28059049/evaluation-of-the-3m%C3%A2-molecular-detection-assay-mda-2-listeria-monocytogenes-for-the-detection-of-listeria-monocytogenes-in-select-foods-and-environmental-surfaces-collaborative-study-first-action-2016-08
#11
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Lisa Monteroso
The 3M<sup>™</sup> Molecular Detection Assay (MDA) 2 - Listeria uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect Listeria spp. in a broad range of food types and environmental surfaces. Using an unpaired study design, the MDA 2 - Listeria was compared to the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook Chapter 8.09 "Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products, and Environmental Samples" reference method for the detection of Listeria in deli turkey and raw chicken breast fillet...
November 17, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28050388/natural-alternatives-for-chemicals-used-in-histopathology-lab-a-literature-review
#12
REVIEW
Ananthalakshmi Ramamoorthy, Shivani Ravi, Nadeem Jeddy, Radhika Thangavelu, Sunitha Janardhanan
Histopathology lab is the place where the specimen gets processed and stained to view under microscope for interpretation. Exposure to the chemicals used in these processes cause various health hazards to the laboratory technicians, pathologists, and scientists working in the laboratory. Hence, there is a dire need to introduce healthy and bio-friendly alternatives in the field. This literature review explores the natural products and their efficiency to be used as alternatives for chemicals in the histopathology lab...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28024819/randomized-controlled-within-subject-evaluation-of-digital-and-conventional-workflows-for-the-fabrication-of-lithium-disilicate-single-crowns-part-ii-cad-cam-versus-conventional-laboratory%C3%A2-procedures
#13
Irena Sailer, Goran I Benic, Vincent Fehmer, Christoph H F Hämmerle, Sven Mühlemann
STATEMENT OF PROBLEM: Clinical studies are needed to evaluate the entire digital and conventional workflows in prosthetic dentistry. PURPOSE: The purpose of the second part of this clinical study was to compare the laboratory production time for tooth-supported single crowns made with 4 different digital workflows and 1 conventional workflow and to compare these crowns clinically. MATERIAL AND METHODS: For each of 10 participants, a monolithic crown was fabricated in lithium disilicate-reinforced glass ceramic (IPS e...
December 23, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#14
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
December 23, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/28011231/metabolic-syndrome-and-occupation-any-association-prevalence-among-auto-technicians-and-school-teachers-in-south-west-nigeria
#15
Adeseye A Akintunde, Taiwo W Oloyede
AIMS: To determine occupational association with metabolic syndrome among auto technicians and school teachers. METHODS: One hundred and sixty six subjects were selected for this study. Clinical data was obtained while laboratory investigations including plasma glucose and lipid profile were performed. Statistical analysis was done with SPSS 20.0. P<0.05 was taken as statistically significant. RESULTS: School teachers had significantly higher mean total cholesterol and LDL-cholesterol compared to auto technicians...
December 14, 2016: Diabetes & Metabolic Syndrome
https://www.readbyqxmd.com/read/27998378/validation-of-contact-free-sleep-monitoring-device-with-comparison-to-polysomnography
#16
Asher Tal, Zvika Shinar, David Shaki, Shlomi Codish, Aviv Goldbart
STUDY OBJECTIVES: To validate a contact-free system designed to achieve maximal comfort during long-term sleep monitoring, together with high monitoring accuracy. METHODS: We used a contact-free monitoring system (EarlySense, Ltd., Israel), comprising an under-the-mattress piezoelectric sensor and a smartphone application, to collect vital signs and analyze sleep. Heart rate (HR), respiratory rate (RR), body movement, and calculated sleep-related parameters from the EarlySense (ES) sensor were compared to data simultaneously generated by the gold standard, polysomnography (PSG)...
December 16, 2016: Journal of Clinical Sleep Medicine: JCSM: Official Publication of the American Academy of Sleep Medicine
https://www.readbyqxmd.com/read/27977585/neurocognitive-performance-and-physical-function-do-not-change-with-physical-cognitive-mindfulness-training-in-female-laboratory-technicians-with-chronic-musculoskeletal-pain-randomized-controlled-trial
#17
RANDOMIZED CONTROLLED TRIAL
Kenneth Jay, Mikkel Brandt, Mc Schraefel, Markus Due Jakobsen, Emil Sundstrup, Gisela Sjøgaard, Jonas Vinstrup, Lars L Andersen
BACKGROUND: Cognitive and physical performance can be negatively affected by chronic pain. This study evaluates the effect of combined physical-, cognitive-, and mindfulness training (PCMT) on cognitive and physical performance. METHODS: From a large pharmaceutical company in Denmark we randomly allocated 112 female laboratory technicians with chronic upper limb pain to group-based PCMT at the worksite or a reference group for 10 weeks. Neurocognitive performance was measured by the computerized central nervous system vital signs neurocognitive assessment battery...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27960550/ensuring-the-collection-of-high-quality-dried-blood-spot-samples-across-multisite-clinical-studies
#18
Tina Panchal, Neil Spooner, Matthew Barfield
AIM: The quality of quantitative analytical measurements is dependent on the quality of the sample collected, and dried blood spots (DBS) are no exception. As the use of DBS has matured into late-stage clinical drug-development studies, it has become apparent that a simple and straightforward approach in a controlled single-site, first-time-into-human clinic, does not always translate into multicenter clinical studies. Using synthetic blood, a method of training and assessing clinical laboratory staff has been developed to ensure the quality of sampling...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27931315/factors-affecting-the-vocational-calling-of-laboratory-animal-care-and-research-employees
#19
Gregory P Boivin, Ronald J Markert
We surveyed laboratory animal care and research workers to determine the factors affecting their vocational calling. The survey comprised 56 questions in 4 groups: passion, job stability or happiness, work volition, and demographics. We hypothesized that personnel who worked in the field a longer time, were older, had higher education levels, were involved with AALAS, and in higher positions in their organization were more likely to indicate a calling to the laboratory animal care field. In addition, we hypothesized that job satisfaction and classifying one's job as a calling were positively related to organizational support and work volition...
November 2016: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/27906464/methodological-considerations-for-optimizing-and-validating-behavioral-assays
#20
Stacey J Sukoff Rizzo, Jill L Silverman
Preclinical animal models are indispensable tools for translational research for which behavioral characterization and phenotyping are essential to testing hypotheses and for evaluating the potential of novel therapeutic agents to treat diseases. The methods employed for comprehensive behavioral phenotyping and pharmacological experiments are complex and should be conducted exclusively by trained technicians with demonstrated proficiency. The ultimate goal is to identify disease-relevant and translational behavioral endpoints that are robust, reliable, and reproducible, and that can be employed to evaluate potential of novel therapeutic agents to treat disease...
December 1, 2016: Current Protocols in Mouse Biology
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