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https://www.readbyqxmd.com/read/29046166/evaluation-of-mericon-e-coli-o157-screen-plus-and-mericon-e-coli-stec-o-type-pathogen-detection-assays-in-select-foods-collaborative-study-first-action-2017-05
#1
Patrick Bird, M Joseph Benzinger, Benjamin Bastin, Erin Crowley, James Agin, David Goins, Marcia Armstrong
QIAGEN mericon Escherichia coli O157 Screen Plus and mericon E. coliShiga toxin-producing E. coli (STEC) O-Type Pathogen Detection Assays use Real-Time PCR technology for the rapid, accurate detection of E. coli O157 and the “big six” (O26, O45, O103, O111, O121, O145) (non-O157 STEC) in select food types. Using a paired study design, the assays were compared with the U.S. Department of Agriculture, Food Safety Inspection Service Microbiology Laboratory Guidebook Chapter 5.09 reference method for the detection of E...
October 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29037984/effect-of-maf-amplification-on-treatment-outcomes-with-adjuvant-zoledronic-acid-in-early-breast-cancer-a-secondary-analysis-of-the-international-open-label-randomised-controlled-phase-3-azure-big-01-04-trial
#2
Robert Coleman, Andrew Hall, Joan Albanell, Andrew Hanby, Richard Bell, David Cameron, David Dodwell, Helen Marshall, Joël Jean-Mairet, Juan-Carlos Tercero, Federico Rojo, Walter Gregory, Roger R Gomis
BACKGROUND: Adjuvant use of bisphosphonates can reduce the incidence of bone metastases in early breast cancer. Recurrence and survival seem to be improved only in postmenopausal patients, but the underlying mechanisms remain unclear. We investigated whether MAF amplification (a biomarker for bone metastasis) in primary tumours could predict the treatment outcomes of adjuvant zoledronic acid. METHODS: The study population included patients enrolled in the international, open-label, randomised, controlled, phase 3 AZURE trial at eligible UK sites who had stage II or III breast cancer and who gave consent for use of their primary tumour samples...
October 13, 2017: Lancet Oncology
https://www.readbyqxmd.com/read/28990891/-turkish-thoracic-society-national-spirometry-and-laboratory-standards
#3
Gaye Ulubay, Nurdan Köktürk, Aslı Görek Dilektaşlı, Elif Şen, Fatma Çiftci, Tunçalp Demir, Öznur Yıldız, Bilun Gemicioğlu, Sevgi Saryal
The application quality of pulmonary function tests (PFT) carries high importance since it affects the quality of health services. "Chronic Diseases and Risk Factors in Turkey Study," which was published in 2013 showed that only 22.6% of PFTs performed in the secondary care institutions in our country were compatible with the standards. This finding reveals the standardization problem in spirometry applications in our country. Currently, the criteria for the application and evaluation of spirometer measurements were defined by American and European Respiratory Societies standards...
June 2017: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28961150/the-use-of-differential-item-functioning-dif-analysis-to-distinguish-between-similar-job-roles
#4
Nicole M Risk, James R Fidler
Two primary roles in the clinical laboratory are those of Medical Technologist (MT) and Medical Laboratory Technician (MLT). Job analyses, which form the foundation of test blueprints employed for credentialing practitioners, suggest a reasonable amount of overlap in the tasks performed by MTs and MLTs. However, credentialing assessments must clearly distinguish between the two roles and ensure that they address competencies appropriate to each practice designation. Differential item functioning (DIF) analysis techniques were applied to explore and differentiate the two laboratory practitioner job roles as an aspect of examination development...
2017: Journal of Applied Measurement
https://www.readbyqxmd.com/read/28952524/opendrop-an-integrated-do-it-yourself-platform-for-personal-use-of-biochips
#5
Mirela Alistar, Urs Gaudenz
Biochips, or digital labs-on-chip, are developed with the purpose of being used by laboratory technicians or biologists in laboratories or clinics. In this article, we expand this vision with the goal of enabling everyone, regardless of their expertise, to use biochips for their own personal purposes. We developed OpenDrop, an integrated electromicrofluidic platform that allows users to develop and program their own bio-applications. We address the main challenges that users may encounter: accessibility, bio-protocol design and interaction with microfluidics...
May 19, 2017: Bioengineering
https://www.readbyqxmd.com/read/28951037/nanoparticles-in-dentistry
#6
REVIEW
Gottfried Schmalz, Reinhard Hickel, Kirsten L van Landuyt, Franz-Xaver Reichl
OBJECTIVE: Nanoparticles having a size from 1 to 100nm are present in nature and are successfully used in many products of daily life. Nanoparticles are also embedded per se or as byproducts from milling processes of larger filler particles in many dental materials. METHODS AND RESULTS: Recently, possible adverse effects of nanoparticles have gained increased interest with the lungs being a main target organ. Exposure to nanoparticles in dentistry may occur in the dental laboratory, by processing gypsum type products or by grinding and polishing materials...
September 23, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28941351/autovalidation-and-automation-of-the-postanalytical-phase-of-routine-hematology-and-coagulation-analyses-in-a-university-hospital-laboratory
#7
Ana Mlinaric, Marija Milos, Désirée Coen Herak, Mirjana Fucek, Vladimira Rimac, Renata Zadro, Dunja Rogic
BACKGROUND: The need to satisfy high-throughput demands for laboratory tests continues to be a challenge. Therefore, we aimed to automate postanalytical phase in hematology and coagulation laboratory by autovalidation of complete blood count (CBC) and routine coagulation test results (prothrombin time [PT], international normalized ratio [PT-INR], activated partial thromboplastin time [APTT], fibrinogen, antithrombin activity [AT] and thrombin time [TT]). Work efficacy and turnaround time (TAT) before and after implementation of automated solutions will be compared...
September 23, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28918967/accuracy-of-bedside-point-of-care-testing-in-critical-emergency-department-patients
#8
Braden W McIntosh, Jerina Vasek, Maria Taylor, Deborah Le Blanc, Henry C Thode, Adam J Singer
BACKGROUND: Point-of-care (POC) testing reduces laboratory turn-around having the potential to improve timely diagnosis and management. We compared the accuracy of nurse performed POC and core laboratory testing and determined whether deviations between the two were clinically meaningful. METHODS: We performed a prospective, observational study on a convenience sample of 50 critical care ED patients in whom a POC chemistry and hematocrit was ordered. Blood samples were divided into 2 aliquots; one sample was tested by the treating nurse using a handheld POC device and the other sample was tested in the core laboratory...
September 14, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28911343/validation-study-of-maxsignal-%C3%A2-histamine-enzymatic-assay-for-the-detection-of-histamine-in-fish-seafood
#9
Swapna Gone, Nicolas Kosa, Joseph Krebs
Bioo Scientific Corp. has developed a rapid enzymatic quantitative assay for the determination of histamine inseafood. Fresh/frozen tuna, canned tuna, pouched tuna, and frozen mahi mahi samples were used for the validation study under the specific guidelines of the AOAC Research Institute Performance Tested Methods(SM) program. Recoveries ranged from 82 to 107% at concentrations ranging from 6 to 72 ppm, with RSDr values between 0.8 and 6.5% (6-72 ppm). The linearity of the assay ranged from 0 to 108 ppm, with R² values exceeding 0...
September 15, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28894572/comparison-between-digital-and-optical-microscopy-analysis-in-a-mouse-gut-inflammation-model
#10
Airton Pereira E Silva, Sylvia Maria Nicolau Campos, Isabelle Mazza Guimarães, Gerlinde Agate Platais Brasil Teixeira
Virtual microscopy is currently widely used for various purposes, such as teaching, archiving, collaborations and research. Although the cost of this technique has reduced, it continues to be expensive for the majority of laboratories in developing countries. The Graduate Program in Pathology at the Federal Fluminense University (Niterói, Brazil) has acquired equipment for virtual microscopy. However, this novel method faced prejudice, as students and technicians were skeptical about its reliability. Thus, the aim of the current study was to evaluate whether virtual microscopy is a reliable method of analysis for our research...
September 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28888410/three-dimensional-printing-in-contemporary-fixed-prosthodontics-a-technique-article
#11
Sarah Bukhari, Brian J Goodacre, Abdulaziz AlHelal, Mathew T Kattadiyil, Paul M Richardson
Digital dentistry has gained in popularity among clinicians and laboratory technicians because of its versatile applications. Three-dimensional (3D) printing has been applied in many areas of dentistry as it offers efficiency, affordability, accessibility, reproducibility, speed, and accuracy. This article describes a technique where 3D printing is used to fabricate a die-trimmed cast and to replicate gingival tissue and implant analogs. The digital workflow that replaces the conventional laboratory procedure is outlined...
September 6, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28887958/photoelastic-analysis-of-mandibular-full-arch-implant-supported-fixed-dentures-made-with-different-bar-materials-and-manufacturing-techniques
#12
Danilo Zaparolli, Raniel Fernandes Peixoto, Denise Pupim, Ana Paula Macedo, Marcelo Bighetti Toniollo, Maria da Glória Chiarello de Mattos
PURPOSE: To compare the stress distribution of mandibular full dentures supported with implants according to the bar materials and manufacturing techniques using a qualitative photoelastic analysis. MATERIAL AND METHODS: An acrylic master model simulating the mandibular arch was fabricated with four Morse taper implant analogs of 4.5×6mm. Four different bars were manufactured according to different material and techniques: fiber-reinforced resin (G1, Trinia, CAD/CAM), commercially pure titanium (G2, cpTi, CAD/CAM), cobalt‑chromium (G3, Co-Cr, CAD/CAM) and cobalt‑chromium (G4, Co-Cr, conventional cast)...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28877182/cryptococcal-antigen-screening-by-lay-cadres-using-a-rapid-test-at-the-point-of-care-a-feasibility-study-in-rural-lesotho
#13
Fernanda Rick, Aline Aurore Niyibizi, Amir Shroufi, Kazumi Onami, Sarah-Jane Steele, Malehlohonolo Kuleile, Innocent Muleya, Tom Chiller, Tiffany Walker, Gilles Van Cutsem
INTRODUCTION: Cryptococcal meningitis is one of the leading causes of death among people with HIV in Africa, primarily due to delayed presentation, poor availability and high cost of treatment. Routine cryptococcal antigen (CrAg) screening of patients with a CD4 count less than 100 cells/mm3, followed by pre-emptive therapy if positive, might reduce mortality in high prevalence settings. Using the cryptococcal antigen (CrAg) lateral flow assay (LFA), screening is possible at the point of care (POC)...
2017: PloS One
https://www.readbyqxmd.com/read/28872292/jerzy-pi%C3%A4-tkowski-1929-1992-%C3%A2-laboratory-technician-inscribed-in-history-of-the-department-of-epidemiology-%C3%A2-in-twenty-fifth-anniversary-of-death
#14
Danuta Naruszewicz-Lesiuk
No abstract text is available yet for this article.
2017: Przegla̧d Epidemiologiczny
https://www.readbyqxmd.com/read/28859722/validation-of-a-new-system-using-tracheal-body-sound-and-movement-data-for-automated-apnea-hypopnea-index-estimation
#15
Christoph Kalkbrenner, Manuel Eichenlaub, Stefan Rüdiger, Cornelia Kropf-Sanchen, Rainer Brucher, Wolfgang Rottbauer
STUDY OBJECTIVES: The current gold standard for assessment of obstructive sleep apnea is the in-laboratory polysomnography. This approach has high costs and inconveniences the patient, whereas alternative ambulatory systems are limited by reduced diagnostic abilities (type 4 monitors, 1 or 2 channels) or extensive setup (type 3 monitors, at least 4 channels). The current study therefore aims to validate a simplified automated type 4 monitoring system using tracheal body sound and movement data...
August 29, 2017: Journal of Clinical Sleep Medicine: JCSM: Official Publication of the American Academy of Sleep Medicine
https://www.readbyqxmd.com/read/28855446/carpal-tunnel-syndrome-among-laboratory-technicians-in-relation-to-personal-and-ergonomic-factors-at-work
#16
Mohamed El-Helaly, Hanan H Balkhy, Laura Vallenius
OBJECTIVES: Work-related carpal tunnel syndrome (CTS) has been reported in different occupations, including laboratory technicians, so this study was carried out to determine the prevalence and the associated personal and ergonomic factors for CTS among laboratory technicians.Methods A cross-sectional study was conducted among 279 laboratory technicians at King Fahd Hospital, Saudi Arabia, who filled in a self-administered questionnaire, including questions regarding their demographic criteria, occupational history, job tasks, workplace tools, ergonomic factors at work, and symptoms suggestive of CTS...
August 31, 2017: Journal of Occupational Health
https://www.readbyqxmd.com/read/28832563/participation-in-interdisciplinary-meetings-on-genetic-diagnostics-ngs
#17
Tom Koole, Lotte van Burgsteden, Paulien Harms, Cleo C van Diemen, Irene M van Langen
Diagnostics using next generation sequencing (NGS) requires high-quality interdisciplinary collaboration. In order to gain insight into this crucial collaborative process, we made video recordings of a new multidisciplinary team at work in the clinical genetics department of the University Medical Centre Groningen. Conversation Analysis was used to investigate the ways in which the team members deal with the disciplinary boundaries between them. We found that the team established different 'participation frames' in which to discuss recurring topics...
October 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28825539/evaluation-of-3m-molecular-detection-assay-mda-2-listeria-for-the-detection-of-listeria-species-in-select-foods-and-environmental-surfaces-collaborative-study-first-action-2016-07
#18
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Lisa Monteroso
3M Molecular Detection Assay (MDA) 2-Listeria uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect Listeria species in a broad range of food types and environmental surfaces. Using an unpaired study design, MDA 2-Listeria was compared with the U.S. Department of Agriculture, Food Safety and Inspection Service's Microbiology Laboratory Guidebook Chapter 8.09 "Isolation and identification of Listeria monocytogenes from red meat, poultry and egg products, and environmental samples" reference method for the detection of Listeria in deli turkey and raw chicken breast fillet...
January 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28815426/identification-and-red-blood-cell-automated-counting-from-blood-smear-images-using-computer-aided-system
#19
Vasundhara Acharya, Preetham Kumar
Red blood cell count plays a vital role in identifying the overall health of the patient. Hospitals use the hemocytometer to count the blood cells. Conventional method of placing the smear under microscope and counting the cells manually lead to erroneous results, and medical laboratory technicians are put under stress. A computer-aided system will help to attain precise results in less amount of time. This research work proposes an image-processing technique for counting the number of red blood cells. It aims to examine and process the blood smear image, in order to support the counting of red blood cells and identify the number of normal and abnormal cells in the image automatically...
August 17, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28804839/platelet-function-analyzed-by-light-transmission-aggregometry
#20
Anne-Mette Hvas, Emmanuel J Favaloro
Analysis of platelet function is widely used for diagnostic work-up in patients with increased bleeding tendency. During the last decades, platelet function testing has also been introduced for evaluation of antiplatelet therapy, but this is still recommended for research purposes only. Platelet function can also be assessed for hyper-aggregability, but this is less often evaluated. Light transmission aggregometry (LTA) was introduced in the early 1960s and has since been considered the gold standard. This optical detection system is based on changes in turbidity measured as a change in light transmission, which is proportional to the extent of platelet aggregation induced by addition of an agonist...
2017: Methods in Molecular Biology
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