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https://www.readbyqxmd.com/read/28527280/-pperformance-of-led-fluorescence-microscopy-for-the-detection-of-acid-fast-bacilli-from-respiratory-samples-in-peripheral-laboratories-in-argentina
#1
María Imaz, Sonia Allassia, Mónica Aranibar, Alba Gunia, Susana Poggi, Ana Togneri, Lidia Wolff, Group Of Implementation Of Fluorescence
INTRODUCTION: Light-emitting diode fluorescence microscopy (LED-FM) has been endorsed by the World Health Organization (WHO) for tuberculosis diagnosis, but its accuracy in HIV-infected patients remains controversial, and only some few studies have explored procedural factors that may affect its performance. OBJECTIVE: To evaluate the performance of LED-FM for tuberculosis diagnosis in patients with and without HIV infection using a newer, less expensive LED lamp...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28508922/adaptation-and-optimization-of-a-fluorescence-based-assay-for-in-vivo-antimalarial-drug-screening
#2
Maria H Arias, Eric Deharo, Alexis Valentin, Giovanny Garavito
The in vivo efficacy of potential antimalarials is usually evaluated by direct microscopic determination of the parasitaemia of Plasmodium-infected mice on Giemsa-stained blood smears. This process is time-consuming, requires experienced technicians and is not automatable. Therefore, we optimized a SYBR Green I (SYBRG I) fluorescence-based assay to fluorometers commonly available in many research laboratories. This technique was originally developed to assess parasitaemia in humans by cytometry. We defined optimal conditions with Plasmodium berghei-infected mice, standard lysis buffer (Tris, EDTA, saponin and Triton), whole blood cells and 2 h staining incubation with SYBRG I 2X...
May 16, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28496253/survey-on-the-use-of-cad-cam-technology-by-uk-and-irish-dental-technicians
#3
E Blackwell, M Nesbit, H Petridis
Statement of the problem Digital workflows (CAD/CAM) have been introduced in dentistry during recent years. No published information exists on dental technicians' use and reporting of this technology.Purpose The aim of this cross sectional survey was to identify the extent digital technology has infiltrated the workplace and to investigate the factors affecting the use of CAD-CAM technology by dental laboratory technicians within Ireland and the UK.Materials and methods A web-based questionnaire was composed (Opinio, Object Planet Inc...
May 12, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28491303/self-evaluation-of-tobacco-exposure-by-allied-health-students-in-a-community-college-setting
#4
Fiona Geiser, Meredith Much, Karen Beyer
OBJECTIVE: Tobacco education is among the initiatives encouraged for health professionals to help them appreciate the significance of addressing tobacco use among their patients. In this pilot study, a nicotine biomarker (cotinine) study was introduced to an applied microbiology course required of all allied health students. Participants assessed their own smoking status in the previous 24 h using a two-dimensional experimental design of a questionnaire and rapid urinary cotinine immunoassay...
2017: SAGE Open Medicine
https://www.readbyqxmd.com/read/28490345/post-2015-why-delay-to-seek-healthcare-perceptions-and-field-experiences-from-tb-healthcare-providers-in-northern-malawi-a-qualitative-study
#5
Nathan B W Chimbatata, Chang-Ming Zhou, Chikondi M Chimbatata, Biao Xu
BACKGROUND: Malawi is a low-income country with high Tuberculosis (TB) burden. TB diagnosis delay and untimely initiation of treatment is still a major problem in Malawi which could increase the risk of tuberculosis transmission in the communities. This study investigated factors related to the diagnostic delay of tuberculosis from TB healthcare providers in the northern region of Malawi. METHODS: Nine focus group discussions were conducted with 57 participants in total...
May 11, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28478985/a-multicenter-randomized-controlled-clinical-trial-comparing-the-use-of-displacement-cords-an-aluminum-chloride-paste-and-a-combination-of-paste-and-cords-for-tissue-displacement
#6
Erna R Einarsdottir, Niklaus P Lang, Thor Aspelund, Bjarni E Pjetursson
STATEMENT OF PROBLEM: Gingival recession after soft tissue displacement for impression making in fixed prosthodontics may pose a problem for treatment success in the esthetic areas of the mouth. Knowledge about the soft tissue reaction of common gingival displacement methods is limited. PURPOSE: The purpose of this clinical randomized controlled trial (RCT) was to evaluate changes in the marginal soft tissue height with 3 different gingival tissue displacement techniques for definitive impression making of natural teeth...
May 5, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28467309/automated-antinuclear-immunofluorescence-antibody-analysis-is-a-reliable-approach-in-routine-clinical-laboratories
#7
Bing Zheng, Enling Li, Haoming Zhu, Jingbo Lu, Xinming Shi, Jie Zhang, Min Li
BACKGROUND: Indirect immunofluorescence (IIF) assays are recommended as the gold standard method for the detection of antinuclear antibodies (ANAs). This study aimed to investigate the reliability of an automated system. METHODS: We compared 3745 serum samples using NOVA View archived images with manual analysis via microscopy. A custom cutoff value was established to distinguish ANA titers and was validated in two clinical laboratories. The automatic ANA pattern recognition system was evaluated, and all ANA-positive sera were subjected to two commercial ANA IIF kits to compare the consistency of the pattern interpretation results...
May 3, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28462405/monolithic-zirconia-reconstructions-supported-by-teeth-and-implants-1-to-3-year-results-of-a-case-series
#8
Andreas Worni, Joannis Katsoulis, Lumni Kolgeci, Mathias Worni, Regina Mericske-Stern
OBJECTIVE: Today, only scarce information is available on monolithic zirconia reconstructions. The objective of this study was to evaluate the performance of monolithic zirconia for tooth- and implant-borne reconstructions. METHOD AND MATERIALS: Monolithic zirconia single crowns (SCs) and fixed dental prostheses (FDPs) supported by implants or teeth were included in this study. Implant placement and prosthetic treatment were done in the same clinical setting. One technician performed all laboratory work using the same CAD/CAM workflow (DentalDesigner, Ceramill Motion 2, Amann Girrbach)...
April 28, 2017: Quintessence International
https://www.readbyqxmd.com/read/28448206/mycoplasma-pulmonis-of-rodents-as-a-possible-human-pathogen
#9
Tomasz Piasecki, Klaudia Chrzastek, Urszula Kasprzykowska
Mycoplasma pulmonis is a naturally occurring respiratory pathogen in rodents. To date, this pathogen was not isolated from humans. This study aimed to evaluate the prevalence and seropositivity to M. pulmonis in humans who have had direct contact with rats. Moreover, the prevalence of M. pulmonis in pet and laboratory rats was assessed. Overall, 131 and 235 oropharyngeal swab samples were collected from human individuals and rats, respectively. In humans, M. pulmonis was detected by PCR in 21 of 86 pet rat keepers (24...
April 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28409856/pleural-malignant-mesothelioma-in-dental-laboratory-technicians-a-case-series
#10
Carolina Mensi, Francesco Ciullo, Gino Pietro Barbieri, Luciano Riboldi, Anna Somigliana, Giulio Rasperini, Angela Cecilia Pesatori, Dario Consonni
Asbestos was used in dentistry as a binder in periodontal dressings and as lining material for casting rings and crucible. However, until now, only one case of malignant mesothelioma with occupational exposure to asbestos in dental practice has been reported. We present 4 pleural mesotheliomas out of 5344 cases identified in Lombardy, Italy, in 2000-2014. Three men had been working as dental laboratory technicians, with asbestos exposure for 10, 34, and 4 years, and one woman had been helping her husband for 30 years in manufacturing dental prostheses...
May 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/28397029/introduction-to-moral-and-other-laboratories
#11
EDITORIAL
Teresa Kuan, Lone Grøn
"Moral (and other) laboratories" is a special issue that draws on Cheryl Mattingly's notion of the "moral laboratory" to explore the uncanny interface between laboratory ethnography and moral anthropology, and to examine the relationship between experience and experiment. We ask whether laboratory work may provoke new insights about experimental practices in other social spaces such as homes, clinics, and neighborhoods, and conversely, whether the study of morality may provoke new insights about laboratory practices as they unfold in the day-to-day interactions between test tubes, animals, apparatuses, scientists, and technicians...
April 10, 2017: Culture, Medicine and Psychiatry
https://www.readbyqxmd.com/read/28381724/current-status-of-regulatory-science-education-in-faculties-of-pharmaceutical-science-in-japan
#12
REVIEW
Masahiro Tohkin
 I introduce the current pharmaceutical education system in Japan, focusing on regulatory science. University schools or faculties of pharmaceutical science in Japan offer two courses: a six-year course for pharmacists and a four-year course for scientists and technicians. Students in the six-year pharmaceutical course receive training in hospitals and pharmacies during their fifth year, and those in the four-year life science course start research activities during their third year. The current model core curriculum for pharmaceutical education requires them to "explain the necessity and significance of regulatory science" as a specific behavior object...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28366143/-where-do-we-perform-sleep-test-in-the-laboratory-or-at-home
#13
Handan İnönü Köseoğlu, Asiye Kanbay, Turan Acıcan
Obstructive sleep apnea syndrome (OSAS) is an important public health problem seen in 14.3% of male, and 5% of female population which leads to higher morbidity, and mortality rates because of its outcomes effecting many organ systems including cardiovascular, and cerebrovascular systems. Despite its higher prevalence in community, sleep laboratories both in our country, and in the world remain incapable of diagnosing these patients, and 80-90% of the cases with OSAS can not be diagnosed. As an alternative to gold standard polysomnographic examinations this desperate condition revived the use of more practical, and less expensive portable monitorization devices which can be used at home without the need for help of a technician...
December 2016: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28362689/collaboration-patterns-and-processes-between-dentists-and-dental-laboratories-when-planning-and-fabricating-implant-supported-restorations
#14
Noga Harel, Avi Meirowitz, Jonathan Block, Ady Palti, Shlomo Matalon, Zeev Ormianer
INTRODUCTION: The success of implant-supported restorations is dependent on proper treatment planning, effective communication within the clinical team, and the use of appropriate methods and materials in the dental laboratory. The objective of this study was to determine collaboration trends between dentists and laboratories and to assess the common methods and materials involved in fabricating implant-supported restorations. METHODS: Questionnaires were distributed to dental laboratories and technicians...
March 29, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28334777/clinicians-interpretations-of-point-of-care-urine-culture-versus-laboratory-culture-results-analysis-from-the-four-country-poetic-trial-of-diagnosis-of-uncomplicated-urinary-tract-infection-in-primary-care
#15
Saskia Hullegie, Mandy Wootton, Theo J M Verheij, Emma Thomas-Jones, Janine Bates, Kerenza Hood, Micaela Gal, Nick A Francis, Paul Little, Michael Moore, Carl Llor, Timothy Pickles, David Gillespie, Nigel Kirby, Curt Brugman, Christopher C Butler
Background.: Urine culture at the point of care minimises delay between obtaining the sample and agar inoculation in a microbiology laboratory, and quantification and sensitivity results can be available more rapidly in primary care. Objective.: To identify the degree to which clinicians' interpretations of a point-of-care-test (POCT) urine culture (Flexicult™ SSI-Urinary Kit) agrees with laboratory culture in women presenting to primary care with symptoms of uncomplicated urinary tract infections (UTI)...
March 1, 2017: Family Practice
https://www.readbyqxmd.com/read/28316309/high-resolution-ct-angiogram-for-lower-extremity-vein-mapping
#16
Mark P Wright, Matthew R Smeds, Lonnie Wright, Ahsan T Ali
High-resolution CT angiogram (CTA) has replaced traditional diagnostic angiography as the main preoperative imaging modality for vascular surgery patients. Although the use of CTA is increasing for arterial imaging, it has not been used routinely for vein mapping. The goal of this study was to evaluate the accuracy of CTA for venous anatomy and compare it to a standard venous duplex ultrasound (DUS). When the vein was used for bypass, the quality and size of the vein were evaluated in the operating room. As part of a preoperative workup before lower extremity revascularization, 16 patients underwent a CTA followed by a DUS...
March 1, 2017: American Surgeon
https://www.readbyqxmd.com/read/28296974/joint-analyses-of-open-comments-and-quantitative-data-added-value-in-a-job-satisfaction-survey-of-hospital-professionals
#17
Ingrid Gilles, Mauro Mayer, Nelly Courvoisier, Isabelle Peytremann-Bridevaux
OBJECTIVE: To obtain a comprehensive understanding of the job opinions of hospital professionals by conducting qualitative analyses of the open comments included in a job satisfaction survey and combining these results with the quantitative results. DESIGN: A cross-sectional survey targeting all Lausanne University Hospital professionals was performed in the fall of 2013. MATERIAL AND METHODS: The survey considered ten job satisfaction dimensions (e...
2017: PloS One
https://www.readbyqxmd.com/read/28273211/spirometry-reference-values-in-the-brazilian-population
#18
R Rufino, C H Costa, A J Lopes, A I Maiworm, K Maynard, L M R A Silva, R M Dias
The aim of the present study was to provide new spirometry reference equations in a sample of the Brazilian population for the following parameters: forced vital capacity (FVC), forced expiratory volume in 1 second (FEV1), FEV1/FVC ratio, peak of expiratory flow (PEF), forced expiratory flow at 50% (FEF50%), 75% average vital capacity (FEF25-75%), and average forced expiratory flow time (FEFT). This was a prospective study using results from chest radiographs, electrocardiograms, and questionnaires to investigate the participants' respiratory symptoms, sedentarism, and comorbidities (Charlson comorbidity index)...
March 2, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28268987/identifying-individual-sleep-apnea-hypoapnea-epochs-using-smartphone-based-pulse-oximetry
#19
Ainara Garde, Parastoo Dekhordi, J Mark Ansermino, Guy A Dumont
Sleep apnea, characterized by frequent pauses in breathing during sleep, poses a serious threat to the healthy growth and development of children. Polysomnography (PSG), the gold standard for sleep apnea diagnosis, is resource intensive and confined to sleep laboratories, thus reducing its accessibility. Pulse oximetry alone, providing blood oxygen saturation (SpO2) and blood volume changes in tissue (PPG), has the potential to identify children with sleep apnea. Thus, we aim to develop a tool for at-home sleep apnea screening that provides a detailed and automated 30 sec epoch-by-epoch sleep apnea analysis...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28268025/knowledge-attitude-and-practice-kap-of-teaching-laboratory-technicians-towards-laboratory-safety-and-waste-management-a-pilot-interventional-study
#20
A-H El-Gilany, S El-Shaer, E Khashaba, S A El-Dakroory, N Omar
A quasi-experimental study was performed on 20 technicians working in the Faculty of Medicine, Mansoura University, Egypt. The knowledge, attitude, and practice (KAP) of laboratory technicians was measured before and two months after enrolling them in an intervention programme about laboratory best practice procedures. The programme addressed laboratory safety and medical waste management. The assessment was performed using a validated Arabic self-administered questionnaire. Pre- and post-intervention scores were compared using non-parametric tests...
February 9, 2017: Journal of Hospital Infection
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