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https://www.readbyqxmd.com/read/29431262/the-japanese-society-of-pathology-guidelines-on-the-handling-of-pathological-tissue-samples-for-genomic-research-standard-operating-procedures-based-on-empirical-analyses
#1
Yae Kanai, Hiroshi Nishihara, Yohei Miyagi, Tatsuhiro Tsuruyama, Kenichi Taguchi, Hiroto Katoh, Tomoyo Takeuchi, Masahiro Gotoh, Junko Kuramoto, Eri Arai, Hidenori Ojima, Ayako Shibuya, Teruhiko Yoshida, Toshiaki Akahane, Rika Kasajima, Kei-Ichi Morita, Johji Inazawa, Takeshi Sasaki, Masashi Fukayama, Yoshinao Oda
Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed "The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research" based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci...
February 2018: Pathology International
https://www.readbyqxmd.com/read/29427686/laboratory-diagnosis-of-urinary-tract-infections-towards-a-bilulu-consensus-guideline
#2
REVIEW
Matthijs Oyaert, Britt Van Meensel, Reinoud Cartuyvels, Johan Frans, Wim Laffut, Patricia Vandecandelaere, Hans De Beenhouwer
The BILULU study group consists of seven microbiologists of hospital laboratories located in the region of Flanders (Belgium). A major goal of the group is to standardize diagnostic microbiology procedures based on available evidence and, in the absence of evidence, based on general microbiological principles and expert opinion. Scientific evidence of current urine culture guidelines is incomplete and at some points guidelines don't indicate clear choice. The aim of this project was to develop a clear and unambiguous step-by-step guideline regarding the work-up of urine cultures...
February 7, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29422560/aesthetic-possibilities-in-removable-prosthodontics-part-2-start-with-the-face-not-the-teeth-when-rehearsing-lip-support-and-tooth-positions
#3
J N Besford, A F Sutton
Even dentures exhibiting superb aesthetics are of no use if they visibly move during speech and social intercourse. In this, the second paper of three on removable denture aesthetics, we describe impression making and shaping the wax occlusal record rims. Not only are the impressions important for producing dentures with maximum retention, stability and support, but their extensions and the thickness of their borders have a decisive influence on lip support and profile. This article shows how the contours of the definitive impressions and the wax rims are developed so as to prescribe the overall form of the replacement gums and teeth...
February 9, 2018: British Dental Journal
https://www.readbyqxmd.com/read/29422384/efficacy-and-safety-of-primaquine-and-methylene-blue-for-prevention-of-plasmodium-falciparum-transmission-in-mali-a-phase-2-single-blind-randomised-controlled-trial
#4
Alassane Dicko, Michelle E Roh, Halimatou Diawara, Almahamoudou Mahamar, Harouna M Soumare, Kjerstin Lanke, John Bradley, Koualy Sanogo, Daouda T Kone, Kalifa Diarra, Sekouba Keita, Djibrilla Issiaka, Sekou F Traore, Charles McCulloch, Will J R Stone, Jimee Hwang, Olaf Müller, Joelle M Brown, Vinay Srinivasan, Chris Drakeley, Roly Gosling, Ingrid Chen, Teun Bousema
BACKGROUND: Primaquine and methylene blue are gametocytocidal compounds that could prevent Plasmodium falciparum transmission to mosquitoes. We aimed to assess the efficacy and safety of primaquine and methylene blue in preventing human to mosquito transmission of P falciparum among glucose-6-phosphate dehydrogenase (G6PD)-normal, gametocytaemic male participants. METHODS: This was a phase 2, single-blind, randomised controlled trial done at the Clinical Research Centre of the Malaria Research and Training Centre (MRTC) of the University of Bamako (Bamako, Mali)...
February 5, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29402397/task-shifting-of-cd4-t-cell-count-monitoring-by-the-touchscreen-based-muse%C3%A2-auto-cd4-cd4-single-platform-system-for-cd4-t-cell-numeration-implication-for-decentralization-in-resource-constrained-settings
#5
André Kouabosso, Christian Diamant Mossoro-Kpinde, Ralph-Sydney Mboumba Bouassa, Jean De Dieu Longo, Marcel Mbeko Simaleko, Gérard Grésenguet, Laurent Bélec
BACKGROUND: The accuracy of CD4 T cell monitoring by the recently developed flow cytometry-based CD4 T cell counting Muse™ Auto CD4/CD4% Assay analyzer (EMD Millipore Corporation, Merck Life Sciences, KGaA, Darmstadt, Germany) was evaluated in trained lay providers against laboratory technicians. METHODS: After 2 days of training on the Muse™ Auto CD4/CD4% analyzer, EDTA-blood samples from 6 HIV-positive and 4 HIV-negative individuals were used for CD4 T cell counting in triplicate in parallel by 12 trained lay providers as compared to 10 lab technicians...
February 2, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29402350/evaluation-of-volume-of-intramuscular-injection-into-the-caudal-thigh-muscles-of-female-and-male-balb-c-mice-mus-musculus
#6
Alicia M Gehling, Kyle Kuszpit, Emily J Bailey, Krystal H Allen-Worthington, David P Fetterer, Pedro J Rico, Thomas M Bocan, Christian C Hofer
This study presents recommendations for intramuscular injection into the caudal thigh muscle of mice according to analysis of in vivo imaging of intramuscularly injected iohexol, a radiocontrast agent commonly used in CT imaging. An experienced laboratory animal technician using a Hamilton syringe intramuscularly injected iohexol into isoflurane-anesthetized female and male BALB/c mice. Injected volumes (25, 50, 100, and 200 μL) underwent CT scanning at 9 time points over a 3-h period. The distribution of the injectate in the muscles of the rear leg was examined over time for each volume group...
January 1, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/29399267/pseudocellulitis-need-not-be-benign-three-cases-of-superficial-migratory-thrombophlebitis-with-negative-venous-duplex-ultrasonography
#7
Frances Tian, Katherine R Mazurek, Robert N Malinak, Steven M Dean, Benjamin H Kaffenberger
Trousseau syndrome is a rare phenomenon in cancer patients characterized by superficial migratory thrombophlebitis. In this brief report, the authors describe three recent case presentations of patients without a prior history of cancer who were treated for cellulitis prior to be admitted to the hospital. All three patients were found to have "negative" testing on venous duplex scanning. Communication with the technicians and additional clinical and laboratory evaluations confirmed Trousseau syndrome as well as an underlying hematologic cancer in each patient...
December 2017: Journal of Clinical and Aesthetic Dermatology
https://www.readbyqxmd.com/read/29392688/whole-genome-dna-methylation-analysis-of-human-glioblastoma-using-illumina-beadarrays
#8
Jonathan Serrano, Matija Snuderl
In this chapter, we describe the use of Illumina® Infinium® HD Assay in conjunction with Illumina's EPIC Methylation 8-sample array platform to obtain glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue and is compatible with an Illumina iScan or HiScan system.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29390513/concomitant-systemic-lupus-erythematosus-and-hiv-infection-a-rare-case-report-and-literature-review
#9
Hong-Yan Liao, Chuan-Min Tao, Jun Su
RATIONALE: Coexisting systemic lupus erythematosus (SLE) and human immunodeficiency virus (HIV) infection cases are rare worldwide. Great challenges are posed on the diagnosis and treatment of such concurrent cases. PATIENT CONCERN: We report the case of a 44-year-old Chinese man with edema, hematuria, and fever who presented at West China Hospital, Sichuan University, Chengdu, Sichuan, China, in 2013. DIAGNOSES: An initial diagnosis of SLE was made from the clinical manifestations and laboratory findings based on the Systemic Lupus International Collaborating Clinics classification criteria...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29380127/pathologist-s-assistant-patha-and-his-her-role-in-the-surgical-pathology-department-a-systematic-review-and-a-narrative-synthesis
#10
M Bortesi, V Martino, M Marchetti, A Cavazza, G Gardini, E Zanetti, M C Bassi, L Ghirotto, M Costantini, Simonetta Piana
In recent decades, various highly qualified individuals have increasingly performed tasks that have historically been handled by physicians with the aim of reducing their workload. Over time, however, these "physician assistants" or "physician extenders" have gained more and more responsibilities, showing that specific tasks can be performed equally skilfully by specialised health care professionals. The pathologist's assistant (PathA) is a highly qualified technician who works alongside the pathologist and is responsible for the grossing and autopsies...
January 29, 2018: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/29379902/conservative-restoration-of-the-worn-dentition-the-anatomically-driven-direct-approach-ada
#11
Rui Negrão, Jorge André Cardoso, Nuno Braz de Oliveira, Paulo Julio Almeida, Teresa Taveira, Oleg Blashkiv
The treatment or management of tooth wear with composite resins can be challenging because significant alterations in the patient's occlusion are usually required. Comprehensive approaches include the use of facebows, articulator-mounted casts, laboratory-made wax-ups, and silicone indexes to deliver the restorations. Even though this sequence of steps is recommended, in many cases it is not applied. The reason for this is related to the complexity and time required for these steps, which are normally applied in indirect restorations like ceramics but not properly used in direct composite resin rehabilitations...
2018: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/29371195/clinical-support-role-for-a-pharmacy-technician-within-a-primary-care-resource-center
#12
Toni Fera, Keith T Kanel, Meghan L Bolinger, Amber E Fink, Serah Iheasirim
PURPOSE: The creation of a clinical support role for a pharmacy technician within a primary care resource center is described. SUMMARY: In the Primary Care Resource Center (PCRC) Project, hospital-based care transition coordination hubs staffed by nurses and pharmacist teams were created in 6 independent community hospitals. At the largest site, patient volume for targeted diseases challenged the ability of the PCRC pharmacist to provide expected elements of care to targeted patients...
February 1, 2018: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/29346577/improved-success-rate-of-arterial-puncture-for-blood-gas-analysis-through-standardization
#13
Phillip R Jensen, Boaz A Markewitz
Background: Arterial puncture for blood gas analysis is a common procedure in hospitals. The aim of the study is to determine if standardizing technique elements of the arterial puncture process could improve the success rate of technicians through the full scope of an academic medical center. Methods: The study is conducted by the Blood Gas Laboratory at University of Utah Health's main campus. During the baseline period, technicians tallied whether arterial puncture attempts were successful or unsuccessful from December 2014 through February 2015...
January 13, 2018: Laboratory Medicine
https://www.readbyqxmd.com/read/29341939/the-quest-for-equivalence-of-test-results-the-pilgrimage-of-the-dutch-calibration-2-000-program-for-metrological-traceability
#14
REVIEW
Rob T P Jansen, Christa M Cobbaert, Cas Weykamp, Marc Thelen
Calibration 2.000 was initiated 20 years ago for standardization and harmonization of medical tests. The program also intended to evaluate adequate implementation of the In Vitro Diagnostics (IVD) 98/79/EC directive, in order to ensure that medical tests are fit-for-clinical purpose. The Calibration 2.000 initiative led to ongoing verification of test standardization and harmonization in the Netherlands using commutable external quality assessment (EQA)-tools and a type 1 EQA-design, where feasible. National support was guaranteed by involving all laboratory professionals as well as laboratory technicians responsible for EQA and quality officers...
January 17, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29341858/respiratory-assessment-of-refractory-ceramic-fibers-in-a-heating-technician-population
#15
David Lucas, Vincent Clamagirand, Pascale Capellmann, Agnès Hervé, Gilles Mauguen, Yannik Le Mer, Dominique Jegaden
Refractory ceramic fibers (RCF) have been extensively used for insulation in condensing boilers. The aim of this study was to evaluate the respiratory exposure to these fibers among maintenance heating technicians. We first created a working group (Carsat Brittany and Finistère Occupational Health Services) and carried out a sampling strategy. Atmospheric measurements were done during work tasks, and filters were analyzed by phase contrast microscopy (PCM) and scanning electron microscopy (SEM) in French approved laboratories...
January 17, 2018: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/29331571/interdepartmental-imaging-protocol-for-clinically-based-three-dimensional-computed-tomography-can-provide-accurate-measurement-of-glenoid-version
#16
Connor L Zale, Gregory I Pace, Gregory S Lewis, John Chan, H Mike Kim
BACKGROUND: Conventional computed tomography (CT) is not accurate for glenoid version measurement. This study sought to examine the feasibility of an interdepartmental protocol implemented between orthopedic surgery and radiology departments for acquisition of anatomic axial CT images and to validate the glenoid version measured through such a protocol. MATERIALS AND METHODS: Data of 30 conventional CT scans of 10 normal and 20 osteoarthritic glenoids were transferred to clinical 3-dimensional imaging software by a radiology technician trained for the study...
January 10, 2018: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29328443/-retracted-effects-of-cyclin-e-gene-silencing-on-the-proliferation-of-esophageal-cancer-cell-lines-ec9706-eca109-and-kyse30
#17
Na Wang, Min Li, Wen-Qiao Zang, Yun-Yun Ma, Yuan-Yuan Wang, Guo-Qiang Zhao
Subsequently to the publication of this article, an interested reader drew to our attention the fact that the six panels shown in Fig. 6 shared several areas of identity among them. Following an internal investigation, a laboratory technician, who was responsible for editing the pictures, admitted that the data as presented in the figure had been manipulated after having mislaid some of the original data. The corresponding author of the article takes responsibility for this oversight, and therefore the paper is to be retracted from publication...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29304039/establishing-ebola-virus-disease-evd-diagnostics-using-genexpert-technology-at-a-mobile-laboratory-in-liberia-impact-on-outbreak-response-case-management-and-laboratory-systems-strengthening
#18
Philomena Raftery, Orla Condell, Christine Wasunna, Jonathan Kpaka, Ruth Zwizwai, Mahmood Nuha, Mosoka Fallah, Maxwell Freeman, Victoria Harris, Mark Miller, April Baller, Moses Massaquoi, Victoria Katawera, John Saindon, Philip Bemah, Esther Hamblion, Evelyn Castle, Desmond Williams, Alex Gasasira, Tolbert Nyenswah
The 2014-16 Ebola Virus Disease (EVD) outbreak in West Africa highlighted the necessity for readily available, accurate and rapid diagnostics. The magnitude of the outbreak and the re-emergence of clusters of EVD cases following the declaration of interrupted transmission in Liberia, reinforced the need for sustained diagnostics to support surveillance and emergency preparedness. We describe implementation of the Xpert Ebola Assay, a rapid molecular diagnostic test run on the GeneXpert platform, at a mobile laboratory in Liberia and the subsequent impact on EVD outbreak response, case management and laboratory system strengthening...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29299350/non-clinical-models-for-neurodegenerative-diseases-therapeutic-approach-and-drug-validation-in-animal-models
#19
Caridad Ivette Fernandez, Jessica López, Lázara Martinez
In 2016, 19.8% of the Cuban population was aged 60 or over. As a result, age-associated degenerative diseases and other diseases have become priority targets from a prophylactic, diagnostic and therapeutic perspective. As a result, the Cuban biomedical scientific community has addressed its basic, preclinical and epidemiological research in order to rise up to the challenge. A firm step in this direction has been the international congress "State of the art in non-clinical models for neurodegenerative diseases" which has brought together preclinical and clinical researchers, technicians and regulatory staff members from different countries to review the state of the art in neurodegenerations, find unifying ideas, objectives and collaborations or partnership...
December 2017: Behavioral Sciences
https://www.readbyqxmd.com/read/29291537/comparative-evaluation-of-the-certest-viasure-flu-a-b-rsv-real-time-rt-pcr-detection-kit-on-the-bd-max-system-versus-a-routine-in-house-assay-for-detection-of-influenza-a-and-b-virus-during-the-2016-17-influenza-season
#20
Thomas Vognbjerg Sydenham, Malene Bek-Thomsen, Signe Dalsgaard Andersen, Birte Kolmos, Ea Sofie Marmolin, Ramona Trebbien, Jens Kjølseth Møller
BACKGROUND: Diagnosing of influenza rapidly and accurately helps clinicians to initiate appropriate treatment options and isolation protocols. Unnecessary antimicrobial treatment and laboratory testing can also be reduced. Assess commercial alternatives to in-house assays that may not only reduce laboratory technician "hands on" time but also the laboratory turnaround time is of interest. OBJECTIVES: We evaluated the performance of the VIASURE Flu A, B & RSV Real Time RT-PCR Detection Kit (CerTest Biotec) for detecting Influenza A and B viruses...
December 21, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
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