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https://www.readbyqxmd.com/read/27906464/methodological-considerations-for-optimizing-and-validating-behavioral-assays
#1
Stacey J Sukoff Rizzo, Jill L Silverman
Preclinical animal models are indispensable tools for translational research for which behavioral characterization and phenotyping are essential to testing hypotheses and for evaluating the potential of novel therapeutic agents to treat diseases. The methods employed for comprehensive behavioral phenotyping and pharmacological experiments are complex and should be conducted exclusively by trained technicians with demonstrated proficiency. The ultimate goal is to identify disease-relevant and translational behavioral endpoints that are robust, reliable, and reproducible, and that can be employed to evaluate potential of novel therapeutic agents to treat disease...
December 1, 2016: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/27904162/impact-of-catheterization-lab-computer-software-settings-on-hemodynamic-assessment-of-aortic-stenosis
#2
Zaher Fanari, Anitha Rajamanickam, Mathew Grove, Sumaya Hammami, Cassie Walls, Paul Kolm, William Weintraub, Andrew J Doorey
BACKGROUND: Accurate assessment of Cardiac Output (CO) is a critical measurement in the calculation of aortic valve area (AVA). Due to the known inaccuracy of estimated Fick calculations, many measure thermodilution (TD) CO as well due to previous studies showing better correlation with the gold standard direct CO. Previous studies showed suboptimal correlation between both methods. Most physicians assume that the TD CO is chosen by catheterization laboratory software for AVA evaluation...
July 2016: Delaware Medical Journal
https://www.readbyqxmd.com/read/27891473/a-comparison-of-conventional-and-microwave-decalcification-and-processing-of-tooth-and-mandibular-bone-specimens
#3
A Thirumal Raj, Shankargouda Patil, Roopa S Rao
INTRODUCTION: Any laboratory procedure exposes the technician and the pathologists to the hazardous effects of chemicals. Conventional procedures like decalcification and histo-processing employed in laboratories are labour intense and time consuming thereby delaying the report dispatch. The present study was an attempt to employ a kitchen microwave to hasten the process and facilitate faster and accurate reporting; thus, benefitting the technician, pathologist and the patient. AIM: To compare conventional and microwave based decalci-fication, processing and staining of tooth and mandibular bone specimens using 5% nitric acid as decalcifying agent...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27885097/results-of-relid-study-2014-buenos-aires-argentina-retrospective-evaluation-of-lens-injuries-and-dose
#4
C Papp, M Romano-Miller, A Descalzo, S Michelin, A Molinari, A Rossini, C Plotkin, G Bodino, G Esperanza, M Di Giorgio, R Touzet
High levels of scatter radiation in catheterization laboratories may lead to posterior subcapsular opacities in the lens of the staff. The international Retrospective Evaluation of Lens Injuries and Dose (RELID) was performed in Argentina for the first time in 2010 in the context of the congress of the Latin American Society of Interventional Cardiology (SOLACI) and recently, in 2014, was carried out for the second time (SOLACI-CACI 2014). The 2014 study included 115 participants: interventional cardiologists, technicians and nurses...
November 24, 2016: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27884215/public-perception-of-emergency-medical-services-in-the-united-states
#5
Remle P Crowe, Roger Levine, Severo Rodriguez, Ashley D Larrimore, Ronald G Pirrallo
OBJECTIVE: The objective of this study was to assess the public's experience, expectations, and perceptions related to Emergency Medical Services (EMS). METHODS: A population-based telephone interview of adults in the United States was conducted. The survey instrument consisted of 112 items. Demographic variables including age, race, political beliefs, and household income were collected. Data collection was performed by trained interviewers from Kent State University's (Kent, Ohio USA)Social Research Laboratory...
November 25, 2016: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/27872822/automating-hesc-differentiation-with-3d-printing-and-legacy-liquid-handling-solutions
#6
Eric Zluhan, Kathleen Kelly, Nick LeClair, Danique Wortel, Kesley Moody
Historically, the routine use of laboratory automation solutions has been prohibitively expensive for many laboratories. As legacy hardware has begun to emerge on the secondary market, automation is becoming an increasingly affordable option to augment workflow in virtually any laboratory. To assess the utility of legacy liquid handling in stem cell differentiation, a used liquid handling robot was purchased at auction to automate a stem cell differentiation protocol that gives rise to CD14 + CD45+ mononuclear cells...
2016: MethodsX
https://www.readbyqxmd.com/read/27870167/design-of-complete-dentures-by-adopting-cad-developed-for-fixed-prostheses
#7
Yanfeng Li, Weili Han, Jing Cao, Yuan Iv, Yue Zhang, Yishi Han, Yi Shen, Zheng Ma, Huanyue Liu
The demand for complete dentures is expected to increase worldwide, but complete dentures are mainly designed and fabricated manually involving a broad series of clinical and laboratory procedures. Therefore, the quality of complete dentures largely depends on the skills of the dentist and technician, leading to difficulty in quality control. Computer-aided design and manufacturing (CAD/CAM) has been used to design and fabricate various dental restorations including dental inlays, veneers, crowns, partial crowns, and fixed partial dentures (FPDs)...
November 21, 2016: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/27848921/staphylococcus-aureus-with-panton-valentine-toxin-skin-infection-in-a-medical-laboratory-technician
#8
Richard Pougnet, Laurence Pougnet
This report exposes the case of a Staphylococcus aureus infection occurring in a microbiology laboratory technician. He was a 52 year-old man without medical history. He presented an abscess on the anterior aspect of the left forearm. Analysis showed that it was a Staphylococcus aureus secreting the Panton-Valentine toxin. The study of the workplace found the frequency of exposure. The study of workstation showed the link between the technician position and the infection. Indeed, this man touched an area where the biocleaning was hard to do...
December 1, 2016: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/27843164/implementation-research-a-mentoring-programme-to-improve-laboratory-quality-in-cambodia
#9
Lucy A Perrone, Vireak Voeurng, Sophat Sek, Sophanna Song, Nora Vong, Chansamrach Tous, Jean-Frederic Flandin, Deborah Confer, Alexandre Costa, Robert Martin
OBJECTIVE: To implement a mentored laboratory quality stepwise implementation (LQSI) programme to strengthen the quality and capacity of Cambodian hospital laboratories. METHODS: We recruited four laboratory technicians to be mentors and trained them in mentoring skills, laboratory quality management practices and international standard organization (ISO) 15189 requirements for medical laboratories. Separately, we trained staff from 12 referral hospital laboratories in laboratory quality management systems followed by tri-weekly in-person mentoring on quality management systems implementation using the LQSI tool, which is aligned with the ISO 15189 standard...
October 1, 2016: Bulletin of the World Health Organization
https://www.readbyqxmd.com/read/27829809/bridging-theory-and-practice-mixed-methods-approach-to-instruction-of-law-and-ethics-within-the-pharmaceutical-sciences
#10
Kyle John Wilby, Ziad Ghantous Nasr
Background: Professional responsibilities are guided by laws and ethics that must be introduced and mastered within pharmaceutical sciences training. Instructional design to teaching typically introduces concepts in a traditional didactic approach and requires student memorization prior to application within practice settings. Additionally, many centers rely on best practices from abroad, due to lack of locally published laws and guidance documents. Objectives: The aim of this paper was to summarize and critically evaluate a professional skills laboratory designed to enhance learning through diversity in instructional methods relating to pharmacy law and best practices regarding narcotics, controlled medications, and benzodiazepines...
November 2016: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/27821977/viral-hepatitis-and-assisted-reproduction
#11
EDITORIAL
Gautam N Allahbadia
Infertility treatment in couples where one or both parents are infected with hepatitis raises many concerns about transmission of the infection to the baby, laboratory technicians, and medical staff, and contamination of other gametes/embryos that are from virus-free parents in the same laboratory. Exposure to the other partner is only a risk when the couple's hepatitis status is discordant. The management of infertility, in association with HBV or HCV, has sparked debates about the potential risk of spread of infection to virus-free individuals, embryos, and/or semen...
December 2016: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/27808351/die-cad-cam-gefertigte-totalprothese-mit-dem-%C3%A2-digital-denture-professional-system%C3%A2
#12
Tim Joda, Philipp Müller, Frank Zimmerling, Martin Schimmel
The article describes the medical and dental procedures for four treatment sessions with the Digital Denture Professional (DDP) system. First, conventional preliminary dental impressions of the full arch are taken to achieve accurate jaw positioning records. The study models and patient data are digitalised using a laboratory scanner. The dental lab technician then constructs and mills custom impression trays and a mount as an intraoral instrument. A closed-mouth impression is then taken to record vertical dimensions and central relation position...
2016: Swiss Dental Journal
https://www.readbyqxmd.com/read/27761283/efficiency-of-direct-microscopy-of-stool-samples-using-an-antigen-specific-adhesin-test-for-entamoeba-histolytica
#13
Arzu İrvem, Kamil Özdil, Zuhal Çalışkan, Muhterem Yücel
BACKGROUND: E. histolytica is among the common causes of acute gastroenteritis. The pathogenic species E. histolytica and the nonpathogenic species E. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. In many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using Native-Lugol's iodine for pre-diagnosis. AIMS: In this study, we aimed to investigate the frequency of E...
September 2016: Balkan Medical Journal
https://www.readbyqxmd.com/read/27747626/current-status-of-implant-prosthetics-in-japan-a-survey-among-certified-dental-lab-technicians
#14
Yoshiyuki Hagiwara, Tatsuya Narita, Yohei Shioda, Keisuke Iwasaki, Takayuki Ikeda, Shunsuke Namaki, Thomas J Salinas
BACKGROUND: There are many implant cases in which dental technicians take initiative with regard to the design of implant prostheses, and to a certain extent, this area of care is one in which dentists do not necessarily play the leading role. Moreover, inadequate communication between dental technicians and dentists and insufficient instructions for technicians has been highlighted as issues in the past. The purpose of this questionnaire is to improve the quality of implant prostheses and thereby contribute to patient service by clarifying, among other aspects of treatment, problem areas and considerations in the fabrication of implant prostheses, conceptual-level knowledge, and awareness of prosthodontics on the part of the dentists in charge of treatment and methods for preventing prosthetic complications...
December 2015: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/27746602/knowledge-attitude-and-practice-of-dental-professionals-regarding-the-effect-and-management-of-food-impaction-associated-with-fixed-partial-denture-prostheses-a-survey
#15
Aradhana Nagarsekar, Ridhima Gaunkar, Meena Aras
OBJECTIVE: This survey was undertaken to assess dentist's opinion regarding the occurrence and pattern of food impaction in relation to fixed partial denture (FPD) prostheses, its commonly observed consequences, factors contributing to it, and its management. METHODS: A descriptive survey was conducted on a sample size of 150 dental practitioners. The pro forma consisted of informed consent, demographic information, and questionnaire. The results were tallied and quantitative analysis was performed to obtain the descriptive statistics for the data using SPSS version 20...
October 2016: Journal of Indian Prosthodontic Society
https://www.readbyqxmd.com/read/27745763/the-first-time-seizure-emergency-department-electroencephalogram-study
#16
Andrew J Wyman, Bruce N Mayes, Jackeline Hernandez-Nino, Nigel Rozario, Sandra K Beverly, Andrew W Asimos
STUDY OBJECTIVE: Seizures account for 1.2% of all emergency department (ED) visits, with 24% of those representing first-time seizures. Our primary goal is to determine whether obtaining an electroencephalogram (EEG) in the ED after a first-time seizure can identify individuals appropriate for initiation of anticonvulsant therapy on ED discharge. Our secondary goals are to determine the association of historical and clinical seizure features with epileptic EEGs and to determine the interobserver agreement for the EEG interpretation...
October 10, 2016: Annals of Emergency Medicine
https://www.readbyqxmd.com/read/27721674/a-biological-safety-cabinet-certification-program-experiences-in-southeast-asia
#17
Toni Whistler, Anek Kaewpan, Stuart D Blacksell
Biological safety cabinets (BSCs) are the primary means of containment used in laboratories worldwide for the safe handling of infectious microorganisms. They provide protection to the laboratory worker and the surrounding environment from pathogens. To ensure the correct functioning of BSCs, they need to be properly maintained beyond the daily care routines of the laboratory. This involves annual maintenance and certification by a qualified technician in accordance to the NSF/American National Standards Institute 49-2014 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification...
September 2016: Applied Biosafety: Journal of the American Biological Safety Association
https://www.readbyqxmd.com/read/27717600/opportunities-and-challenges-when-pooling-milk-samples-using-elisa
#18
Kaare Græsbøll, Lars Ole Andresen, Tariq Halasa, Nils Toft
Testing large quantities of samples in order to detect one or more test-positive sample(s) is expensive and time-consuming. It is possible to optimize this process by pooling samples. Two frameworks to produce different hierarchical and non-hierarchical pooling schemes were tested and compared to standard pooling. Their efficiency and the potential savings were determined as a function of prevalence and the number of pooled samples. The potential benefit of pooling samples is dependent upon the changes in the analytical sensitivity and specificity of the test used when diluting test-positive samples by pooling...
September 29, 2016: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/27698276/human-toxocariasis-prevalence-and-factors-associated-with-biosafety-in-research-laboratories
#19
Gabriela Torres Mattos, Paula Costa Dos Santos, Paula de Lima Telmo, Maria Elisabeth Aires Berne, Carlos James Scaini
Human toxocariasis is a neglected parasitic disease worldwide. Researchers studying this disease use infectious strains of Toxocara for experiments. Health workers are at risk in the course of their daily routine and must adhere to biosafety standards while carrying out the activities. Researchers on biosafety concerning working with these parasites are insufficient. The aim of this study was to determine the rate of seroprevalence of Toxocara species among health-care research laboratory workers (professors, technicians, and students), and to investigate the risk factors of Toxocara infection associated with laboratory practices...
October 3, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27696539/inter-laboratory-validation-of-organism-recovery-for-use-in-42-day-sediment-toxicity-tests-with-hyalella-azteca
#20
Lisa N Taylor, Lesley Novak
Environment and Climate Change Canada (ECCC) has developed a 42 day sediment toxicity test that includes a reproduction endpoint with the freshwater amphipod Hyalella azteca. The new methodology conducts the entire exposure in sediment; in contrast to existing standardized methods where adults are transferred to a water-only exposure before release of their first brood at Day 28. This mid-test transfer to clean water was due to the results of a juvenile H. azteca recovery trial conducted in the 1990s which concluded that reproductive endpoints could be biased because of low recovery of young amphipods from sediment...
October 3, 2016: Environmental Toxicology and Chemistry
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