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https://www.readbyqxmd.com/read/29161329/comparative-metabolomics-profiling-of-engineered-saccharomyces-cerevisiae-lead-to-a-strategy-that-improving-%C3%AE-carotene-production-by-acetate-supplementation
#1
Xiao Bu, Liang Sun, Fei Shang, Guoliang Yan
A comparative metabolomic analysis was conducted on recombinant Saccharomyces cerevisiae strain producing β-carotene and the parent strain cultivated with glucose as carbon source using gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography-mass spectrometry (HPLC-MS) and ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) based approach. The results showed that most of the central intermediates associated with amino acids, carbohydrates, glycolysis and TCA cycle intermediates (acetic acid, glycerol, citric acid, pyruvic acid and succinic acid), fatty acids, ergosterol and energy metabolites were produced in a lower amount in recombinant strain, as compared to the parent strain...
2017: PloS One
https://www.readbyqxmd.com/read/29161033/carbon-quantum-dots-grafted-antifouling-membranes-for-osmotic-power-generation-via-pressure-retarded-osmosis-process
#2
Die Ling Zhao, Subhabrata Das, Tai-Shung Chung
Osmotic power generated by pressure-retarded osmosis (PRO) has attracted global attention as a clean, abundant and renewable energy resource. However, the substrates of PRO membranes are particularly prone to fouling because of their direct contact with various foulants in raw water. This leads to a significant decline in power density and impedes the commercialization of PRO technology. In this work, a facile surface modification method has been developed to obtain a new type of nanoparticle functionalized antifouling PRO membranes...
November 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29158670/interferential-current-sensory-stimulation-through-the-neck-skin-improves-airway-defense-and-oral-nutrition-intake-in-patients-with-dysphagia-a-double-blind-randomized-controlled-trial
#3
Keisuke Maeda, Takayuki Koga, Junji Akagi
Background: Neuromuscular electrical stimulation with muscle contraction, administered through the skin of the neck, improves a patient's swallowing ability. However, the beneficial effects of transcutaneous electrical sensory stimulation (TESS), without muscle contraction, are controversial. We investigated the effect of TESS, using interferential current, in patients undergoing dysphagia rehabilitation. Methods: This double-blind, randomized controlled trial involved 43 patients who were prescribed in-hospital dysphagia rehabilitation for ≥3 weeks...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/29157838/impaired-citric-acid-cycle-in-nondiabetic-chronic-kidney-disease
#4
Zhanjun Jia
No abstract text is available yet for this article.
November 14, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29156417/the-effect-of-magnesium-hydroxide-containing-dentifrice-using-an-extrinsic-and-intrinsic-erosion-cycling-model
#5
Vanara Florêncio Passos, Lidiany Karla Azevedo Rodrigues, Sérgio Lima Santiago
OBJECTIVE: To evaluate, in vitro, the effect of Mg(OH)2 dentifrice, and the influence of the number of experimental days, on the extrinsic (citric acid -CA) and intrinsic (hydrochloric acid -HCl) enamel erosion models. DESIGN: Human enamel slabs were selected according to surface hardness and randomly assigned to 3 groups (n=9) as follows: non-fluoridated (negative control), NaF (1450ppm F- positive control) and Mg(OH)2 (2%) dentifrices. The slabs were daily submitted to a 2-h period of pellicle formation and, over a period of 5days, submitted to cycles (3×/day) of erosive challenge (CA 0...
November 14, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29152696/degradation-of-the-earthy-and-musty-odorant-2-4-6-tricholoroanisole-by-persulfate-activated-with-iron-of-different-valences
#6
Kejia Zhang, Xinyan Zhou, Tuqiao Zhang, Lang Yu, Zheming Qian, Wenchao Liao, Cong Li
2,4,6-Trichloroanisole (TCA) is an odorous compound that is often detected in tap water and is difficult to be removed via water treatment. In this study, the transformation efficiency of TCA in the presence of persulfate (PS) activated by iron (Fe(2+), Fe(0), and Fe(3+)) was investigated for the first time. The effects of the activator dosage, oxidant dosage, pH, dosing method, chelating agents, humic acid, and temperature were evaluated. The degradation rate of TCA increased with increasing PS dosages (0...
November 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29151887/an-accurate-description-of-aspergillus-niger-organic-acid-batch-fermentation-through-dynamic-metabolic-modelling
#7
Daniel J Upton, Simon J McQueen-Mason, A Jamie Wood
Background: Aspergillus niger fermentation has provided the chief source of industrial citric acid for over 50 years. Traditional strain development of this organism was achieved through random mutagenesis, but advances in genomics have enabled the development of genome-scale metabolic modelling that can be used to make predictive improvements in fermentation performance. The parent citric acid-producing strain of A. niger, ATCC 1015, has been described previously by a genome-scale metabolic model that encapsulates its response to ambient pH...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29147985/deciphering-the-growth-organic-acid-exudations-and-ionic-homeostasis-of-amaranthus-viridis-l-and-portulaca-oleracea-l-under-lead-chloride-stress
#8
Muhammad Tariq Javed, Muhammad Sohail Akram, Noman Habib, Kashif Tanwir, Qasim Ali, Nabeel Khan Niazi, Huma Gul, Naeem Iqbal
Lead (Pb) stress adversely affects in planta nutrient homeostasis and metabolism when present at elevated concentration in the surrounding media. The present study was aimed at investigation of organic acid exudations, elemental contents, growth, and lipid peroxidation in two wild plants (Amaranthus viridis L. and Portulaca oleracea L.), exhibiting differential root to shoot Pb translocation, under Pb stress. Plants were placed in soil spiked with lead chloride (PbCl2) concentrations of 0, 15, 30, 45, or 60 mg Pb/kg soil, in rhizoboxes supplied with nylon nets around the roots...
November 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29147094/physicochemical-properties-of-gelatin-extracted-from-buffalo-hide-pretreated-with-different-acids
#9
Sri Mulyani, Francis M C Sigit Setyabudi, Yudi Pranoto, Umar Santoso
The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29146334/impact-of-fluorescent-lighting-on-the-browning-potential-of-model-wine-solutions-containing-organic-acids-and-iron
#10
Paris Grant-Preece, Celia Barril, Leigh M Schmidtke, Andrew C Clark
Model wine solutions containing organic acids, individually or combined, and iron(III), were exposed to light from fluorescent lamps or stored in darkness for four hours. (-)-Epicatechin was then added, and the solutions incubated in darkness for 10days. Browning was monitored by UV-visible absorption spectrophotometry and UHPLC-DAD. The pre-irradiated solutions containing tartaric acid exhibited increased yellow/brown coloration compared to the dark controls mainly due to reaction of the tartaric acid photodegradation product glyoxylic acid with (-)-epicatechin to form xanthylium cation pigments...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29145748/application-of-hot-melt-extrusion-for-improving-bioavailability-of-artemisinin-a-thermolabile-drug
#11
C Kulkarni, A L Kelly, T Gough, V Jadhav, K K Singh, A Paradkar
Hot melt extrusion has been used to produce a solid dispersion of the thermolabile drug artemisinin. Formulation and process conditions were optimized prior to evaluation of dissolution and biopharmaceutical performance. Soluplus(®), a low Tg amphiphilic polymer especially designed for solid dispersions enabled melt extrusion at 110 °C although some drug-polymer incompatibility was observed. Addition of 5% citric acid as a pH modifier was found to suppress the degradation. The area under plasma concentration time curve (AUC0-24h) and peak plasma concentration (Cmax) were four times higher for the modified solid dispersion compared to that of pure artemisinin...
November 16, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/29145088/phosphorus-recovery-and-leaching-of-trace-elements-from-incinerated-sewage-sludge-ash-issa
#12
Le Fang, Jiang-Shan Li, Ming Zhi Guo, C R Cheeseman, Daniel C W Tsang, Shane Donatello, Chi Sun Poon
Chemical extraction of phosphorus (P) from incinerated sewage sludge ash (ISSA) is adversely influenced by co-dissolution of metals and metalloids. This study investigated P recovery and leaching of Zn, Cu, Pb, As and Ni from ISSA using inorganic acids (sulphuric acid and nitric acid), organic acids (oxalic acid and citric acid), and chelating agents (ethylenediaminetetraacetic acid (EDTA) and ethylene diamine tetramethylene phosphonate (EDTMP)). The aim of this study was to optimize a leaching process to recover P-leachate with high purity for P fertilizer production...
November 8, 2017: Chemosphere
https://www.readbyqxmd.com/read/29145056/environmental-life-cycle-assessment-of-marine-sediment-decontamination-by-citric-acid-enhanced-microwave-heating
#13
Pietro P Falciglia, Carlo Ingrao, Guido De Guidi, Alfio Catalfo, Guglielmo Finocchiaro, Marcello Farina, Maria Liali, Giuseppe Lorenzano, Gaetano Valastro, Federico G A Vagliasindi
The potential ability of microwave heating (MWH) for the remediation of marine sediments affected by severe hydrocarbon (HC) contamination was investigated. Decontamination effectiveness and environmental sustainability through a comparative Life Cycle Assessment (LCA) were addressed. Main results revealed that the application of a 650-W MWH treatment resulted in a rapid (15min) HC removal. A citric acid (CA) dose of 0.1M led to enhanced-HC removals of 76.9, 96.5 and 99.7% after 5, 10 and 15min of MW irradiation, respectively...
November 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29144073/evaluation-of-certain-food-additives
#14
(no author information available yet)
This report represents the conclusions of a Joint FAO/WHO Expert Committee convened to evaluate the safety of various food additives, including flavouring agents, with a view to concluding as to safety concerns and to preparing specifications for identity and purity. The first part of the report contains a general discussion of the principles governing the toxicological evaluation of and assessment of dietary exposure to food additives, including flavouring agents. A summary follows of the Committee’s evaluations of technical, toxicological and dietary exposure data for 10 food additives (Allura Red AC; carob bean gum; lutein esters from Tagetes erecta; octenyl succinic acid (OSA)– modified gum arabic; pectin; Quinoline Yellow; rosemary extract; steviol glycosides; tartrazine; and xanthan gum) and five groups of flavouring agents (alicyclic, alicyclic-fused and aromatic-fused ring lactones; aliphatic and aromatic amines and amides; aliphatic secondary alcohols, ketones and related esters; cinnamyl alcohol and related substances; and tetrahydrofuran and furanone derivatives)...
2017: World Health Organization Technical Report Series
https://www.readbyqxmd.com/read/29143845/pulse-laser-induced-fragmentation-of-carbon-quantum-dots-a-structural-analysis
#15
Han-Wei Chu, Ju-Yi Mao, Chia-Wen Lien, Pang-Hung Hsu, Yu-Jia Li, Jui-Yang Lai, Tai-Chia Chiu, Chih-Ching Huang
Carbon quantum dots (CQDs) have attracted enormous interest in recent years owing to their low cytotoxicity, excellent biocompatibility and strong fluorescence. They have been successfully employed in sensor, bio-imaging, and drug carrier applications. A complete understanding of their core-surface structure is essential for tuning their physical and chemical properties for various applications. Conventional characterizations of CQDs are conducted with electron microscopy or spectroscopy, such as transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and Raman spectroscopy...
November 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/29141191/microbial-lipids-and-added-value-metabolites-production-by-yarrowia-lipolytica-from-pork-lard
#16
Marlene Lopes, Andreia S Gomes, Carla M Silva, Isabel Belo
Yarrowia lipolytica is a suitable microbial platform to convert low-value hydrophobic substrates into microbial oils and other important metabolites. In this work, this yeast species was used to simultaneously synthetize ex novo lipids and produce citric acid and lipase from animal fat (pork lard) adding higher value to the low-cost fatty substrate. The effect of pH, lard concentration, arabic gum concentration and oxygen mass transfer rate (OTR) on lipids accumulation on Y. lipolytica batch cultures was assessed by an experimental design based on Taguchi method...
November 12, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/29136904/a-light-up-fluorescent-probe-for-citrate-detection-based-on-bispyridinum-amides-with-aggregation-induced-emission-feature
#17
Chenchen Liu, Yandi Hang, Tao Jiang, Ji Yang, Xiao Zhang, Jianli Hua
Citrate is an important intermediate in the citric acid cycle, a vital metabolic pathway for animals, plants and bacteria. It is of great significance to detect its levels in human beings because several diseases may cause the abnormal of citrate. In this paper, a new turn-on fluorescent sensor (TPE-Py) using the classic tetraphenylethylene (TPE) as the aggregation-induced emission (AIE) fluorophore and bipyridinium-based amides as the recognition receptor has been synthesized for the detection of citrate. The probe exhibits good selectivity and sensitivity to citrate with a relatively low detection limit (1...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136831/rapid-method-for-determination-of-90-sr-in-seawater-by-liquid-scintillation-counting-with-an-extractive-scintillator
#18
Masaki Uesugi, Ryosuke Watanabe, Hiroaki Sakai, Akihiko Yokoyama
A rapid determination method of (90)Sr is developed for the monitoring of seawater around the Fukushima Daiichi Nuclear Power Plant (FDNPP). Three ideas of chemical separation and measurements to accelerate (90)Sr analysis are investigated. Strontium is co-precipitated in a two-step procedure with hydroxyapatite after the removal of magnesium phosphate in the presence of citric acid. The purification process of strontium is in combination with solid phase extraction disks. One or two sheets of Sr Rad disk and cyclic operations are examined to eliminate interfering substances and secure the exchange capacity...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136806/multivariate-analysis-of-organic-acids-in-fermented-food-from-reversed-phase-high-performance-liquid-chromatography-data
#19
Pablo Mortera, Federico A Zuljan, Christian Magni, Santiago A Bortolato, Sergio H Alarcón
Multivariate calibration coupled to RP-HPLC with diode array detection (HPLC-DAD) was applied to the identification and the quantitative evaluation of the short chain organic acids (malic, oxalic, formic, lactic, acetic, citric, pyruvic, succinic, tartaric, propionic and α-cetoglutaric) in fermented food. The goal of the present study was to get the successful resolution of a system in the combined occurrence of strongly coeluting peaks, of distortions in the time sensors among chromatograms, and of the presence of unexpected compounds not included in the calibration step...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136796/a-multi-purpose-tool-for-food-inspection-simultaneous-determination-of-various-classes-of-preservatives-and-biogenic-amines-in-meat-and-fish-products-by-lc-ms
#20
Luciano Molognoni, Heitor Daguer, Leandro Antunes de Sá Ploêncio, Juliano De Dea Lindner
This paper describes an innovative fast and multipurpose method for the chemical inspection of meat and fish products by liquid chromatography-tandem mass spectrometry. Solid-liquid extraction and low temperature partitioning were applied to 17 analytes, which included large bacteriocins (3.5kDa) and small molecules (organic acids, heterocyclic compounds, polyene macrolides, alkyl esters of the p-hydroxybenzoic acid, aromatic, and aliphatic biogenic amines and polyamines). Chromatographic separation was achieved in 10min, using stationary phase of di-isopropyl-3-aminopropyl silane bound to hydroxylated silica...
February 1, 2018: Talanta
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