keyword
MENU ▼
Read by QxMD icon Read
search

Muscle-skeletal chains

keyword
https://www.readbyqxmd.com/read/29215200/mir-424-5p-reduces-ribosomal-rna-and-protein-synthesis-in-muscle-wasting
#1
Martin Connolly, Richard Paul, Roser Farre-Garros, Samantha A Natanek, Susannah Bloch, Jen Lee, Jose P Lorenzo, Harnish Patel, Cyrus Cooper, Avan A Sayer, Stephen J Wort, Mark Griffiths, Michael I Polkey, Paul R Kemp
BACKGROUND: A loss of muscle mass occurs as a consequence of a range of chronic and acute diseases as well as in older age. This wasting results from an imbalance of protein synthesis and degradation with a reduction in synthesis and resistance to anabolic stimulation often reported features. Ribosomes are required for protein synthesis, so changes in the control of ribosome synthesis are potential contributors to muscle wasting. MicroRNAs (miRNAs) are known regulators of muscle phenotype and have been shown to modulate components of the protein synthetic pathway...
December 7, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29215198/inflammatory-signalling-regulates-eccentric-contraction-induced-protein-synthesis-in-cachectic-skeletal-muscle
#2
Justin P Hardee, Brittany R Counts, Song Gao, Brandon N VanderVeen, Dennis K Fix, Ho-Jin Koh, James A Carson
BACKGROUND: Skeletal muscle responds to eccentric contractions (ECC) with an anabolic response that involves the induction of protein synthesis through the mechanistic target of rapamycin complex 1. While we have reported that repeated ECC bouts after cachexia initiation attenuated muscle mass loss and inflammatory signalling, cachectic muscle's capacity to induce protein synthesis in response to ECC has not been determined. Therefore, we examined cachectic muscle's ability to induce mechano-sensitive pathways and protein synthesis in response to an anabolic stimulus involving ECC and determined the role of muscle signal transducer and activator of transcription 3 (STAT3)/nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) signalling on ECC-induced anabolic signalling...
December 7, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29214748/alterations-in-the-in-vitro-and-in-vivo-regulation-of-muscle-regeneration-in-healthy-ageing-and-the-influence-of-sarcopenia
#3
Joanna Brzeszczyńska, Angelika Meyer, Robin McGregor, Alain Schilb, Simone Degen, Valentina Tadini, Neil Johns, Ramon Langen, Annemie Schols, David J Glass, Ronenn Roubenoff, James A Ross, Kenneth C H Fearon, Carolyn A Greig, Carsten Jacobi
BACKGROUND: Sarcopenia is defined as the age-related loss of skeletal muscle mass and function. While all humans lose muscle with age, 2-5% of elderly adults develop functional consequences (disabilities). The aim of this study was to investigate muscle myogenesis in healthy elderly adults, with or without sarcopenia, compared with middle-aged controls using both in vivo and in vitro approaches to explore potential biomarker or causative molecular pathways associated with sarcopenic versus non-sarcopenic skeletal muscle phenotypes during ageing...
December 6, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29211106/trypanosoma-cruzi-strain-tciv-infects-raccoons-from-illinois
#4
Cailey Vandermark, Elliott Zieman, Esmarie Boyles, Clayton K Nielsen, Cheryl Davis, Francisco Agustín Jiménez
BACKGROUND: The northern limits of Trypanosoma cruzi across the territory of the United States remain unknown. The known vectors Triatoma sanguisuga and T. lecticularia find their northernmost limits in Illinois; yet, earlier screenings of those insects did not reveal the presence of the pathogen, which has not been reported in vectors or reservoir hosts in this state. OBJECTIVES: Five species of medium-sized mammals were screened for the presence of T. cruzi. METHODS: Genomic DNA was isolated from heart, spleen and skeletal muscle of bobcats (Lynx rufus, n = 60), raccoons (Procyon lotor, n = 37), nine-banded armadillos (Dasypus novemcinctus, n = 5), Virginia opossums (Didelphis virginiana, n = 3), and a red fox (Vulpes vulpes)...
January 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29203401/skeletal-muscle-from-aged-american-quarter-horses-shows-impairments-in-mitochondrial-biogenesis-and-expression-of-autophagy-markers
#5
Chengcheng Li, Sarah H White, Lori K Warren, Stephanie E Wohlgemuth
Aging is associated with decreased mitochondrial content and function in skeletal muscle, possibly due to compromised biogenesis and autophagic removal of dysfunctional mitochondria. The aim of this study was to compare markers of mitochondrial content and biogenesis and of autophagy between skeletal muscle from young and aged American Quarter Horses. Citrate synthase protein and mtDNA copy number were decreased in triceps brachii (TB) muscle (P<0.05) from aged horses, suggesting an age-related decline in mitochondrial content...
December 1, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/29203134/proteomic-profiling-of-large-myofibrillar-proteins-from-dried-and-long-term-stored-polyacrylamide-gels
#6
Sandra Murphy, Kay Ohlendieck
A method for the utilization of dried polyacrylamide gels from the pre-proteomic era is described in order to enable the mass spectrometric analysis of long-term stored protein preparations. The in-gel digestion of high-molecular-mass proteins embedded in a 20-year old gel was carried out following gel re-swelling and resulted in the proteomic identification of a large number of proteins, including 3400 kDa titin, 800 kDa nebulin and myosin heavy chains of 220 kDa from rabbit skeletal muscle. These findings demonstrate that dried protein gels from past biochemical analyses can be successfully reused and analyzed by modern and refined mass spectrometric techniques...
December 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29201231/genetic-variation-of-rs3811463-is-associated-with-gestational-diabetes-mellitus-susceptibility
#7
Yun Liu, Zhi-Ping Ge, Li-Zhou Sun, Pei Tong, Hong-Mei Lu
Gestational diabetes mellitus (GDM) is a growing health concern, and it increases the risk of adverse pregnancy outcomes with substantial long-term adverse health impacts on mothers and their offspring. Several studies have revealed specific associations between genetic variants and the risk of GDM. Single nucleotide polymorphisms (SNPs) are the major type of genetic variation in humans. Let-7 microRNA targets are enriched for genes containing SNPs associated with glucose metabolism, including Lin28. In the present study, the effect of T/C variants of rs3811463 (a SNP located near to the let-7 binding site in Lin28) on GDM risk was investigated...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29200393/-skeletal-muscle-aging-and-mitochondrial-dysfunction-an-update
#8
Julie Faitg, Olivier Reynaud, Jean-Philippe Leduc-Gaudet, Gilles Gouspillou
One of the most obvious and deleterious changes occurring with aging is a progressive loss of skeletal muscle mass and strength, a physiological process named sarcopenia. Amongst the multiple theories that have been put forward to explain sarcopenia, the mitochondrial theory of aging, which postulates that the accumulation of mitochondrial dysfunctions with aging plays a causal role in muscle atrophy, has focused intense research effort and attention in the past decades. The generally accepted view of this theory is that, due to the reactive oxygen species (ROS) production inherent to respiratory chain activity, oxidative damage to mitochondrial proteins, lipids and DNA accumulates with aging...
November 2017: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/29193153/aquaporin-4-expression-in-human-skeletal-muscle-fiber-types
#9
Elisa Vizzaccaro, Chiara Terracciano, Emanuele Rastelli, Roberto Massa
Introduction - Aquaporins (AQPs) are a family of transmembrane proteins involved in the maintenance of osmotic gradients. AQP4 is abundant in skeletal muscle, where it seems to be associated with glycolytic metabolism. We investigated the pattern of expression of AQP4 in normal human myofibers relative to the main forms of myosin heavy chain (MHC). Methods - Six normal human muscle biopsies were analyzed by double immunofluorescence for co-expression of AQP4 and slow or fast MHC. Results - A high percentage (64-99%) of MHC-fast positive fibers showed immunoreaction for AQP4...
November 28, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/29190899/targeting-myocyte-specific-enhancer-factor-2d-contributes-to-the-suppression-of-cardiac-hypertrophic-growth-by-mir-92b-3p-in-mice
#10
Zhi-Qin Hu, Jian-Fang Luo, Xue-Ju Yu, Jie-Ning Zhu, Lei Huang, Jing Yang, Yong-Heng Fu, Tao Li, Yu-Mei Xue, Ying-Qing Feng, Zhi-Xin Shan
The role of microRNA-92b-3p (miR-92b-3p) in cardiac hypertrophy was not well illustrated. The present study aimed to investigate the expression and potential target of miR-92b-3p in angiotensin II (Ang-II)-induced mouse cardiac hypertrophy. MiR-92b-3p was markedly decreased in the myocardium of Ang-II-infused mice and of patients with cardiac hypertrophy. However, miR-92b-3p expression was revealed increased in Ang-II-induced neonatal mouse cardiomyocytes. Cardiac hypertrophy was shown attenuated in Ang-II-infused mice received tail vein injection of miR-92b-3p mimic...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29187824/the-implication-of-pgc-1%C3%AE-on-fatty-acid-transport-across-plasma-and-mitochondrial-membranes-in-the-insulin-sensitive-tissues
#11
REVIEW
Elżbieta Supruniuk, Agnieszka Mikłosz, Adrian Chabowski
PGC-1α coactivator plays a decisive role in the maintenance of lipid balance via engagement in numerous metabolic processes (i.e., Krebs cycle, β-oxidation, oxidative phosphorylation and electron transport chain). It constitutes a link between fatty acids import and their complete oxidation or conversion into bioactive fractions through the coordination of both the expression and subcellular relocation of the proteins involved in fatty acid transmembrane movement. Studies on cell lines and/or animal models highlighted the existence of an upregulation of the total and mitochondrial FAT/CD36, FABPpm and FATPs content in skeletal muscle in response to PGC-1α stimulation...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29186180/clonogenic-myogenic-progenitors-expressing-mcam-cd146-are-incorporated-as-adventitial-reticular-cells-in-the-microvascular-compartment-of-human-post-natal-skeletal-muscle
#12
Tiziana Persichini, Alessia Funari, Marco Colasanti, Benedetto Sacchetti
Recent observation identifies subendothelial (mural) cells expressing MCAM, a specific system of clonogenic, self-renewing, osteoprogenitors (a.k.a, "mesenchymal stem cells") in the microvascular compartment of post-natal human bone marrow (BM). In this study, we used MCAM/CD146, as a marker to localize, isolate and assay subendothelial clonogenic cells from the microvasculature of postnatal human skeletal muscle. We show here that these cells share with their BM counterpart, anatomic position (subendothelial/adventitial) and ex vivo clonogenicity (CFU-Fs)...
2017: PloS One
https://www.readbyqxmd.com/read/29185184/changes-in-contractile-and-metabolic-parameters-of-skeletal-muscle-as-rats-age-from-3-to-12-months
#13
Hongyang Xu, Graham D Lamb, Robyn M Murphy
Laboratory rats are considered mature at 3 months despite that musculoskeletal growth is still occurring. Changes in muscle physiological and biochemical characteristics during development from 3 months, however, are not well understood. Whole muscles and single skinned fibres from fast-twitch extensor digitorum longus (EDL) and predominantly slow-twitch soleus (SOL) muscles were examined from male Sprague-Dawley rats (3, 6, 9, 12 months). Ca2+ sensitivity of contractile apparatus decreased with age in both fast- (~ 0...
November 28, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/29182451/protein-recommendations-for-weight-loss-in-elite-athletes-a-focus-on-body-composition-and-performance
#14
Amy Hector, Stuart M Phillips
There exists a large body of scientific evidence to support protein intakes in excess of the recommended dietary allowance (RDA) (0.8g protein/kg/d) to promote the retention of skeletal muscle and loss of adipose tissue during dietary energy restriction. Diet-induced weight loss with as low as possible ratio of skeletal muscle to fat mass loss is a situation we refer to as high quality weight loss. We propose that high quality weight loss is often of importance to elite athletes in order to maintain their muscle (engine) and shed unwanted fat mass, potentially improving athletic performance...
November 28, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29181033/insulin-like-growth-factor-binding-protein-6-alters-skeletal-muscle-differentiation-of-human-mesenchymal-stem-cells
#15
Doaa Aboalola, Victor K M Han
Insulin-like growth factor binding protein-6 (IGFBP-6), the main regulator of insulin-like growth factor-2 (IGF-2), is a component of the stem cell niche in developing muscle cells. However, its role in muscle development has not been clearly defined. In this study, we investigated the role of IGFBP-6 in muscle commitment and differentiation of human mesenchymal stem cells derived from the placenta. We showed that placental mesenchymal stem cells (PMSCs) have the ability to differentiate into muscle cells when exposed to a specific culture medium by expressing muscle markers Pax3/7, MyoD, myogenin, and myosin heavy chain in a stage-dependent manner with the ultimate formation of multinucleated fibers and losing pluripotency-associated markers, OCT4 and SOX2...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29170849/myh7-mutation-associated-with-two-phenotypes-of-myopathy
#16
Nan Li, Zhe Zhao, Hongrui Shen, Qi Bing, Xuan Guo, Jing Hu
The mutations of MYH7 (slow skeletal/β-cardiac myosin heavy chain) are commonly found in familial hypertrophic/dilated cardiomyopathy, and also can cause Laing early-onset distal myopathy (LDM), myosin storage myopathy (MSM), and congenital myopathy with fiber-type disproportion (CFTD). Here we report two cases whose diagnosis was hereditary myopathy according to clinical feature and muscle pathology analysis. High-throughput genomic sequencing (next generation sequencing) was performed to validate the diagnosis...
November 24, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/29162011/skeletal-muscle-injury-biomarkers-assay-qualification-efforts-and-translation-to-the-clinic
#17
Richard A Goldstein
Skeletal muscle (SKM) injury or myopathy results in structural or functional defects in SKMs that can be caused by variety of factors such as (1) genetic, (2) drug-induced, (3) disease progression (cachexia), or (4) aging (sarcopenia). Creatine kinase (CK) and aspartate transaminase (AST) activity assays have been routinely used as SKM injury biomarkers, but they lack sensitivity and tissue specificity. In collaboration with the Predictive Safety Testing Consortium, we evaluated the diagnostic performance of a muscle injury biomarker panel (MIP) compared to CK and AST and their correlation with the histology scores across 34 different rat studies...
October 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/29156133/harnessing-sphingosine-1-phosphate-signaling-and-nanotopographical-cues-to-regulate-skeletal-muscle-maturation-and-vascularization
#18
Jonathan Hung Tsui, Kajohnkiart Janebodin, Nicholas Ieronimakis, David Michael Patrick Yama, Hee Seok Yang, Rakchanok Chavanachat, Aislinn L Hays, Haeshin Lee, Morayma Reyes, Deok-Ho Kim
Despite possessing substantial regenerative capacity, skeletal muscle can suffer from loss of function due to catastrophic traumatic injury or degenerative disease. In such cases, engineered tissue grafts hold the potential to restore function and improve patient quality of life. Requirements for successful integration of engineered tissue grafts with the host musculature include cell alignment that mimics host tissue architecture and directional functionality, as well as vascularization to ensure tissue survival...
November 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/29148051/mechanical-parameters-of-the-molecular-motor-myosin-ii-determined-in-permeabilised-fibres-from-slow-and-fast-skeletal-muscles-of-the-rabbit
#19
Valentina Percario, Simona Boncompagni, Feliciano Protasi, Irene Pertici, Francesca Pinzauti, Marco Caremani
The skeletal muscle exhibits large functional differences depending on the myosin heavy chain (MHC) isoform expressed in its molecular motor myosin II. The differences in the mechanical features of force generation by myosin isoforms are investigated in situ by using fast sarcomere-level mechanical methods in permeabilised fibres (sarcomere length 2.4 μm, temperature 12°C, 4% dextran T-500) from the slow (soleus, containing the MHC-1 isoform) and the fast (psoas, containing the MHC-2X isoform) skeletal muscle of the rabbit...
November 17, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29142257/using-a-quantitative-quadruple-immunofluorescent-assay-to-diagnose-isolated-mitochondrial-complex-i-deficiency
#20
Syeda T Ahmed, Charlotte L Alston, Sila Hopton, Langping He, Iain P Hargreaves, Gavin Falkous, Monika Oláhová, Robert McFarland, Doug M Turnbull, Mariana C Rocha, Robert W Taylor
Isolated Complex I (CI) deficiency is the most commonly observed mitochondrial respiratory chain biochemical defect, affecting the largest OXPHOS component. CI is genetically heterogeneous; pathogenic variants affect one of 38 nuclear-encoded subunits, 7 mitochondrial DNA (mtDNA)-encoded subunits or 14 known CI assembly factors. The laboratory diagnosis relies on the spectrophotometric assay of enzyme activity in mitochondrially-enriched tissue homogenates, requiring at least 50 mg skeletal muscle, as there is no reliable histochemical method for assessing CI activity directly in tissue cryosections...
November 15, 2017: Scientific Reports
keyword
keyword
37939
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"