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https://www.readbyqxmd.com/read/28306190/natural-polymeric-hydrogel-evaluation-for-skeletal-muscle-tissue-engineering
#1
Beth E Pollot, Christopher R Rathbone, Joseph C Wenke, Teja Guda
Although skeletal muscle has a remarkable ability to repair/regenerate after most types of injuries, there is limited regeneration after volumetric muscle loss (VML). A number of scaffold materials have been used in the development of grafts to treat VML, however, there is still a need to better understand the most appropriate material with regards to its ability to maintain mechanical integrity while also supporting myogenesis. Five commonly used natural polymeric materials (Collagen I, Agarose, Alginate, Fibrin, and Collagen Chitosan) used in skeletal muscle tissue engineering grafts were evaluated for their mechanical properties and myogenic capacity...
March 17, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28303003/effects-of-high-intensity-interval-training-and-moderate-intensity-continuous-training-on-glycaemic-control-and-skeletal-muscle-mitochondrial-function-in-db-db-mice
#2
Vivien Chavanelle, Nathalie Boisseau, Yolanda F Otero, Lydie Combaret, Dominique Dardevet, Christophe Montaurier, Geoffrey Delcros, Sébastien L Peltier, Pascal Sirvent
Physical activity is known as an effective strategy for prevention and treatment of Type 2 Diabetes. The aim of this work was to compare the effects of a traditional Moderate Intensity Continuous Training (MICT) with a High Intensity Interval Training (HIIT) on glucose metabolism and mitochondrial function in diabetic mice. Diabetic db/db male mice (N = 25) aged 6 weeks were subdivided into MICT, HIIT or control (CON) group. Animals in the training groups ran on a treadmill 5 days/week during 10 weeks...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28302447/dcebio-facilitates-myogenic-differentiation-via-intermediate-conductance-ca-2-activated-k-channel-activation-in-c2c12-myoblasts
#3
Shoko Tanaka, Yuko Ono, Kazuho Sakamoto
Membrane hyperpolarization is suggested to be a trigger for skeletal muscle differentiation. We investigated whether DCEBIO, an opener of the small/intermediate conductance Ca(2+) activated K(+) (SKCa/IKCa) channels, increase myogenic differentiation in C2C12 skeletal myoblasts. DCEBIO significantly increased myotube formation, protein expression level of myosin heavy chain II, and mRNA expression level of myogenin in C2C12 myoblasts cultured in differentiation medium. DCEBIO induced myotube formation and hyperpolarization were reduced by the IKCa channel blocker TRAM-34, but not by the SKCa channel blocker apamin...
February 17, 2017: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/28284391/neuromuscular-manifestations-in-mitochondrial-diseases-in-children
#4
Andrés Nascimento, Carlos Ortez, Cristina Jou, Mar O'Callaghan, Federico Ramos, Àngels Garcia-Cazorla
Mitochondrial diseases exhibit significant clinical and genetic heterogeneity. Mitochondria are highly dynamic organelles that are the major contributor of adenosine triphosphate, through oxidative phosphorylation. These disorders may be developed at any age, with isolated or multiple system involvement, and in any pattern of inheritance. Defects in the mitochondrial respiratory chain impair energy production and almost invariably involve skeletal muscle and peripheral nerves, causing exercise intolerance, cramps, recurrent myoglobinuria, or fixed weakness, which often affects extraocular muscles and results in droopy eyelids (ptosis), progressive external ophthalmoplegia, peripheral ataxia, and peripheral polyneuropathy...
November 2016: Seminars in Pediatric Neurology
https://www.readbyqxmd.com/read/28281032/mouse-soleus-slow-muscle-shows-greater-intramyocellular-lipid-droplet-accumulation-than-edl-fast-muscle-fiber-type-specific-analysis
#5
Yusuke Komiya, Shoko Sawano, Daisuke Mashima, Riho Ichitsubo, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya
Skeletal muscle is the main tissue of lipid metabolism and accordingly is critical for homeostasis and energy production; however, the determinants of lipid accumulation in skeletal muscle are unknown. Here, we examined whether the soleus muscle (predominantly slow-twitch fibers) has a higher lipid accumulation capacity than that of the extensor digitorum longus (EDL, predominantly fast-twitch fibers) muscle in mice. Soleus and EDL muscles were harvested from male C57BL/6J mice. The mRNA levels of genes involved in fatty acid import and triglyceride synthesis and accumulation were examined in soleus and EDL muscles...
March 9, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28273841/comprehensive-metabolomic-analysis-in-blood-urine-fat-and-muscle-in-men-with-metabolic-syndrome-a-randomized-placebo-controlled-clinical-trial-on-the-effects-of-resveratrol-after-four-months-treatment
#6
Anne Sofie Korsholm, Thomas Nordstrøm Kjær, Marie Juul Ornstrup, Steen Bønløkke Pedersen
Resveratrol possesses several beneficial metabolic effects in rodents, while the effects of resveratrol in humans remain unclear. Therefore, we performed a non-targeted comprehensive metabolomic analysis on blood, urine, adipose tissue, and skeletal muscle tissue in middle-aged men with metabolic syndrome randomized to either resveratrol or placebo treatment for four months. Changes in steroid hormones across all four matrices were the most pronounced changes observed. Resveratrol treatment reduced sulfated androgen precursors in blood, adipose tissue, and muscle tissue, and increased these metabolites in urine...
March 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28270591/decreased-transcriptional-corepressor-p107-is-associated-with-exercise-induced-mitochondrial-biogenesis-in-human-skeletal-muscle
#7
Debasmita Bhattacharya, Mia Ydfors, Meghan C Hughes, Jessica Norrbom, Christopher G R Perry, Anthony Scimè
Increased mitochondrial content is a hallmark of exercise-induced skeletal muscle remodeling. For this process, considerable evidence underscores the involvement of transcriptional coactivators in mediating mitochondrial biogenesis. However, our knowledge regarding the role of transcriptional corepressors is lacking. In this study, we assessed the association of the transcriptional corepressor Rb family proteins, Rb and p107, with endurance exercise-induced mitochondrial adaptation in human skeletal muscle...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28269789/histopathologic-and-biochemical-evidence-for-mitochondrial-disease-among-279-patients-with-severe-statin-myopathy
#8
Tieying Hou, Yilan Li, Weiwei Chen, Reid R Heffner, Georgirene D Vladutiu
BACKGROUND: Statins have well-known benefits in the prevention of cardiovascular disease, however, 7-29% of patients develop muscle side effects and up to 0.5% develop severe symptoms. Mitochondrial dysfunction has been associated with severe statin-induced myopathy (SM); however, there is a paucity of systematic studies in affected individuals. OBJECTIVES: The goal of this study was to combine clinical and laboratory features with quantitative biochemical and histopathologic studies of skeletal muscle biopsies from SM cases to determine what proportion could be attributed to mitochondrial dysfunction and how many of these had primary respiratory chain defects...
2017: Journal of Neuromuscular Diseases
https://www.readbyqxmd.com/read/28257443/analysis-of-large-versus-small-dogs-reveals-three-genes-on-the-canine-x-chromosome-associated-with-body-weight-muscling-and-back-fat-thickness
#9
Jocelyn Plassais, Maud Rimbault, Falina J Williams, Brian W Davis, Jeffrey J Schoenebeck, Elaine A Ostrander
Domestic dog breeds display significant diversity in both body mass and skeletal size, resulting from intensive selective pressure during the formation and maintenance of modern breeds. While previous studies focused on the identification of alleles that contribute to small skeletal size, little is known about the underlying genetics controlling large size. We first performed a genome-wide association study (GWAS) using the Illumina Canine HD 170,000 single nucleotide polymorphism (SNP) array which compared 165 large-breed dogs from 19 breeds (defined as having a Standard Breed Weight (SBW) >41 kg [90 lb]) to 690 dogs from 69 small breeds (SBW ≤41 kg)...
March 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28252568/time-course-of-inflammatory-gene-expression-following-crush-injury-in-murine-skeletal-muscle
#10
Joachim G Voss, Ayelet Goldshmid Shagal, Joyce M Tsuji, James W MacDonald, Theo K Bammler, Fred M Farin, Barbara St Pierre Schneider
BACKGROUND: Early inflammation and secretion of proinflammatory cytokines such as IL-1β, IL-6, and TNF-α act as the key drivers to regulate inflammation after muscle injury. However, the effects of these key proinflammatory drivers in a noninvasive crush injury model are not well known. Understanding these effects is important for treating crush injuries that occur during natural disasters and military conflicts. PURPOSE: We studied the timed mRNA expression of IL-1β, IL-6, and TNF-α in a noninvasive murine crush injury model to further understand their impact on proinflammatory cytokine pathways that are activated within the first 48 hours after a crush muscle injury...
March 2017: Nursing Research
https://www.readbyqxmd.com/read/28251664/preservation-of-skeletal-muscle-mitochondrial-content-in-older-adults-relationship-between-mitochondria-fibre-type-and-high-intensity-exercise-training
#11
Victoria L Wyckelsma, Itamar Levinger, Michael J McKenna, Luke E Formosa, Michael T Ryan, Aaron C Petersen, Mitchell J Anderson, Robyn M Murphy
KEYPOINTS SUMMARY: Ageing is associated with an upregulation of mitochondrial dynamics proteins, mitofusin 2 (Mfn2) and mitochondrial dynamics protein 49 (MiD49) in human skeletal muscle, the increased abundance of Mfn2 is exclusive to Type II muscle fibres. These changes occur despite a similar content of mitochondria, as measured by COXIV, NDUFA9 and complexes in their native states (Blue Native PAGE). Following 12 weeks of High-Intensity Training (HIT), older adults exhibit a robust increase in mitochondria content...
March 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28251466/shortening-speed-dependent-force-potentiation-is-attenuated-but-not-eliminated-in-skeletal-muscles-without-myosin-phosphorylation
#12
William Gittings, Jordan Bunda, Rene Vandenboom
We investigated the influence of shortening speed on concentric force potentiation at different frequencies in muscles devoid of skeletal myosin light chain kinase (skMLCK(-/-)) and unable to phosphorylate myosin. EDL muscles from skMLCK(-/-) mice were activated in vitro (25 °C) across a range of stimulation frequencies (10-100 Hz) during shortening ramps at 0.10, 0.30, or 0.50 of maximum shortening velocity (Vmax) before and after a potentiating stimulus (PS). When collapsed across all frequencies, the PS increased relative (post/pre) concentric force to 1...
March 2, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28251396/polymorphisms-in-ptk2-are-associated-with-skeletal-muscle-specific-force-an-independent-replication-study
#13
Georgina K Stebbings, A G Williams, C I Morse, S H Day
PURPOSE: The aim of the study was to investigate two single nucleotide polymorphisms (SNP) in PTK2 for associations with human muscle strength phenotypes in healthy men. METHODS: Measurement of maximal isometric voluntary knee extension (MVCKE) torque, net MVCKE torque and vastus lateralis (VL) specific force, using established techniques, was completed on 120 Caucasian men (age = 20.6 ± 2.3 year; height = 1.79 ± 0.06 m; mass = 75.0 ± 10...
March 1, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/28248225/age-and-activity-related-differences-in-the-abundance-of-myosin-essential-and-regulatory-light-chains-in-human-muscle
#14
James N Cobley, Zulezwan Ab Malik, James P Morton, Graeme L Close, Ben J Edwards, Jatin G Burniston
Traditional methods for phenotyping skeletal muscle (e.g., immunohistochemistry) are labor-intensive and ill-suited to multixplex analysis, i.e., assays must be performed in a series. Addressing these concerns represents a largely unmet research need but more comprehensive parallel analysis of myofibrillar proteins could advance knowledge regarding age- and activity-dependent changes in human muscle. We report a label-free, semi-automated and time efficient LC-MS proteomic workflow for phenotyping the myofibrillar proteome...
April 8, 2016: Proteomes
https://www.readbyqxmd.com/read/28246337/diacylglycerol-kinase-%C3%AF%C2%B5-deficiency-preserves-glucose-tolerance-and-modulates-lipid-metabolism-in-obese-mice
#15
Louise Mannerås-Holm, Milena Schönke, Joseph T Brozinick, Laurène Vetterli, Hai-Hoang Bui, Philip Sanders, Emmani B M Nascimento, Marie Björnholm, Alexander V Chibalin, Juleen R Zierath
Diacylglycerol kinases (DGKs) catalyze the phosphorylation and conversion of DAG into phosphatidic acid. DGK isozymes have unique primary structures, expression patterns, subcellular localizations, regulatory mechanisms and DAG preferences. DGKε has a hydrophobic segment that promotes its attachment to membranes and shows substrate specificity for DAG with an arachidonoyl acyl chain in the sn-2 position of the substrate. We determined the role of DGKε in the regulation of energy and glucose homeostasis in relation to diet-induced insulin resistance and obesity using DGKε deficient (KO) and wild-type mice...
February 28, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28245809/mesenchymal-stem-cells-and-myoblast-differentiation-under-hgf-and-igf-1-stimulation-for-3d-skeletal-muscle-tissue-engineering
#16
R Witt, A Weigand, A M Boos, A Cai, D Dippold, A R Boccaccini, D W Schubert, M Hardt, C Lange, A Arkudas, R E Horch, J P Beier
BACKGROUND: Volumetric muscle loss caused by trauma or after tumour surgery exceeds the natural regeneration capacity of skeletal muscle. Hence, the future goal of tissue engineering (TE) is the replacement and repair of lost muscle tissue by newly generating skeletal muscle combining different cell sources, such as myoblasts and mesenchymal stem cells (MSCs), within a three-dimensional matrix. Latest research showed that seeding skeletal muscle cells on aligned constructs enhance the formation of myotubes as well as cell alignment and may provide a further step towards the clinical application of engineered skeletal muscle...
February 28, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28241484/mrna-quantification-of-nipbl-isoforms-a-and-b-in-adult-and-fetal-human-tissues-and-a-potentially-pathological-variant-affecting-only-isoform-a-in-two-patients-with-cornelia-de-lange-syndrome
#17
Beatriz Puisac, María-Esperanza Teresa-Rodrigo, María Hernández-Marcos, Carolina Baquero-Montoya, María-Concepción Gil-Rodríguez, Torkild Visnes, Christopher Bot, Paulino Gómez-Puertas, Frank J Kaiser, Feliciano J Ramos, Lena Ström, Juan Pié
Cornelia de Lange syndrome (CdLS) is a congenital developmental disorder characterized by craniofacial dysmorphia, growth retardation, limb malformations, and intellectual disability. Approximately 60% of patients with CdLS carry a recognizable pathological variant in the NIPBL gene, of which two isoforms, A and B, have been identified, and which only differ in the C-terminal segment. In this work, we describe the distribution pattern of the isoforms A and B mRNAs in tissues of adult and fetal origin, by qPCR (quantitative polymerase chain reaction)...
February 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28238968/changes-in-macroautophagy-chaperone-mediated-autophagy-and-mitochondrial-metabolism-in-murine-skeletal-and-cardiac-muscle-during-aging
#18
Jin Zhou, Shu Yun Chong, Andrea Lim, Brijesh K Singh, Rohit A Sinha, Adam B Salmon, Paul M Yen
Aging causes a general decline in cellular metabolic activity, and function in different tissues and whole body homeostasis. However, the understanding about the metabolomic and autophagy changes in skeletal muscle and heart during aging is still limited. We thus examined markers for macroautophagy, chaperone-mediated autophagy (CMA), mitochondrial quality control, as well as cellular metabolites in skeletal and cardiac muscle from young (5 months old) and aged (27 months old) mice. We found decreased autophagic degradation of p62 and increased ubiquitinated proteins in both tissues from aged mice, suggesting a decline in macroautophagy during aging...
February 26, 2017: Aging
https://www.readbyqxmd.com/read/28229639/muscle-plasticity-related-to-changes-in-tubulin-and-%C3%AE-b-crystallin-levels-induced-by-eccentric-contraction-in-rat-skeletal-muscles
#19
H Jee, E Ochi, T Sakurai, J-Y Lim, K Nakazato, H Hatta
We used the model of eccentric contraction of the hindlimb muscle by Ochi et al. to examine the role of eccentric contraction in muscle plasticity. This model aims to focus on stimulated skeletal muscle responses by measuring tissue weights and tracing the quantities of αB-crystallin and tubulin. The medial gastrocnemius muscle (GCM) responded to electrically induced eccentric contraction (EIEC) with significant increases in tissue weight (p < 0.01) and the ratio of tissue weight to body weight (p < 0...
September 2016: Physiol Int
https://www.readbyqxmd.com/read/28223540/akirin2-promotes-slow-myosin-heavy-chain-expression-by-can-nfatc1-signaling-in-porcine-skeletal-muscle-satellite-cells
#20
Xiaoling Chen, Yanliu Luo, Zhiqing Huang, Guangmang Liu, Hua Zhao
The objective of this study was to evaluate the effect of Akirin2 on slow myosin heavy chain (slow MyHC, MyHC I) gene expression and its molecular mechanisms. In this study, we showed that the protein expression of Akirin2 in pig slow oxidative Psoas major muscle is higher than that in fast glycolytic tibialis anterior muscle, suggesting that Akirin2 may play a role in myofiber typing. Knockdown of Akirin2 decreased the MyHC I expression and the calcineurin (CaN) activity, and also decreased the expressions of NFATc1 and MCIP1...
February 16, 2017: Oncotarget
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