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https://www.readbyqxmd.com/read/28644873/circular-rna-expression-profiling-of-human-granulosa-cells-during-maternal-aging-reveals-novel-transcripts-associated-with-assisted-reproductive-technology-outcomes
#1
Jing Cheng, Jia Huang, Suzhen Yuan, Su Zhou, Wei Yan, Wei Shen, Yun Chen, Xi Xia, Aiyue Luo, Da Zhu, Shixuan Wang
Circular RNAs (circRNAs) are a unique class of endogenous RNAs which could be used as potential diagnostic and prognostic biomarkers of many diseases. Our study aimed to investigate circRNA profiles in human granulosa cells (GCs) during maternal aging and to uncover age-related circRNA variations that potentially reflect decreased oocyte competence. CircRNAs in GCs from in vitro fertilization (IVF) patients with young age (YA, ≤ 30 years) and advanced age (AA, ≥ 38 years) were profiled by microarray, and validated in 20 paired samples...
2017: PloS One
https://www.readbyqxmd.com/read/28640859/toxic-effects-of-atrazine-on-porcine-oocytes-and-possible-mechanisms-of-action
#2
Bao Yuan, Shuang Liang, Yong-Xun Jin, Ming-Jun Zhang, Jia-Bao Zhang, Nam-Hyung Kim
Because atrazine is a widely used herbicide, its adverse effects on the reproductive system have been extensively researched. In this study, we investigated the effects of atrazine exposure on porcine oocyte maturation and the possible mechanisms. Our results showed that the rates of oocyte maturation significantly decreased after treatment with 200 μM atrazine in vitro. Atrazine treatment resulted in abnormal spindle morphology but did not affect actin distribution. Atrazine exposure not only triggered a DNA damage response but also decreased MPF levels in porcine oocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28638275/the-application-of-dehydroepiandrosterone-on-improving-mitochondrial-function-and-reducing-apoptosis-of-cumulus-cells-in-poor-ovarian-responders
#3
Li-Te Lin, Peng-Hui Wang, Zhi-Hong Wen, Chia-Jung Li, San-Nung Chen, Eing-Mei Tsai, Jiin-Tsuey Cheng, Kuan-Hao Tsui
Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of DHEA on cumulus cells (CCs). This was a prospective study performed at one tertiary center from January 2015 to March 2016. A total of 131 women who underwent IVF treatment participated, including 59 normal ovarian responders (NORs) and 72 PORs...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28635583/cigarette-smoke-is-associated-with-altered-expression-of-antioxidant-enzymes-in-granulosa-cells-from-women-undergoing-in-vitro-fertilization
#4
Maria Cristina Budani, Erminia Carletti, Gian Mario Tiboni
This study was undertaken to evaluate whether cigarette smoke is associated with changes in the expression of antioxidant enzymes in granulosa cells of women undergoing IVF treatments. For this aim, the expression of three antioxidant enzymes (SOD1, SOD2 and catalase) in non-smokers (n = 20) and smokers (n = 20) was analyzed. There was a statistically significant overexpression of SOD2 and catalase mRNA levels in smokers in comparison with non-smokers. Cigarette smoking was associated with a lower fertilization rate, implantation rate and pregnancy rate in comparison with non-smokers...
June 21, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28635582/protective-effects-of-ethanol-extracts-of-artemisia-asiatica-nakai-ex-pamp-on-ageing-induced-deterioration-in-mouse-oocyte-quality
#5
Hyuk-Joon Jeon, Seung Yeop You, Dong Hyun Kim, Hong Bae Jeon, Jeong Su Oh
Following ovulation, oocytes undergo a time-dependent deterioration in quality referred to as post-ovulatory ageing. Although various factors influence the post-ovulatory ageing of oocytes, oxidative stress is a key factor involved in deterioration of oocyte quality. Artemisia asiatica Nakai ex Pamp. has been widely used in East Asia as a food ingredient and traditional medicine for the treatment of inflammation, cancer, and microbial infections. Recent studies have shown that A. asiatica exhibits antioxidative effects...
June 21, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28632990/shortening-gametes-co-incubation-time-improves-live-birth-rate-for-couples-with-a-history-of-fragmented-embryos
#6
Anne Le Bras, Laetitia Hesters, Vanessa Gallot, Cathie Tallet, Gerard Tachdjian, Nelly Frydman
Short gamete co-incubation (SGCO) consists in decreasing the duration of contact between oocytes and sperm from the standard overnight insemination (SOI) toward 2 hours. However, the effectiveness of this technique to improve in vitro fertilization and embryo transfer (IVF-ET) outcomes remains controversial. Our study was designed to evaluate the efficiency of SGCO in a poor prognosis population with a history of fragmented embryos defined by the presence of at least 50% of the embryos with more than 25% of cytoplasmic fragments...
June 20, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28630101/oviduct-extracellular-vesicles-protein-content-and-their-role-during-oviduct-embryo-cross-talk
#7
Carmen Alminana, Emilie Corbin, Guillaume Tsikis, Agostinho S Alcantara-Neto, Valérie Labas, Karine Reynaud, Laurent Galio, Rustem Uzbekov, Anastasiia S Garanina, Xavier Druart, Pascal Mermillod
Successful pregnancy requires an appropriate communication between the mother and the embryo. Recently, exosomes and microvesicles, both membrane-bound extracellular vesicles (EVs) present in the oviduct fluid have been proposed as key modulators of this unique cross-talk. However, little is known about their content and their role during oviduct-embryo dialogue. Given the known differences in secretions by in vivo and in vitro oviduct epithelial cells (OEC), we aimed at deciphering the oviduct EVs protein content from both sources...
June 19, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28627828/the-relationship-between-the-mthfr-c677t-genotypes-to-serum-anti-m%C3%A3-llerian-hormone-concentrations-and-in-vitro-fertilization-intracytoplasmic-sperm-injection-outcome
#8
Seyedeh Z Shahrokhi, Faranak Kazerouni, Firouzeh Ghaffari, Ali Rahimipour, Mir D Omrani, Arezoo Arabipoor, Ramin Lak, Elaheh T Ghane
BACKGROUND: The expression of the 5,10-methylentetrahydrofolate reductase (MTHFR) gene in human oocytes and preimplantation embryos suggests that the MTHFR gene is involved in folliculogenesis and female reproduction. Considering the importance of the MTHFR gene on female reproduction, the aim of this study was to evalu- ate the influence of MTHFR C677T polymorphism on ovarian marker reserve, particularly serum anti-Müllerian hormone (AMH) levels, and ovarian response as well as clinical pregnancy rates after in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI)...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28627630/gene-expression-profiling-of-human-blastocysts-from-in%C3%A2-vivo-and-rescue-ivm-with-or-without-melatonin-treatment
#9
Yan Hao, Zhiguo Zhang, Dan Han, Yunxia Cao, Ping Zhou, Zhaolian Wei, Mingrong Lv, Dawei Chen
To evaluate the effect of melatonin supplementation in maturation medium for human 'rescue IVM' and investigate differences in transcriptomic profile of blastocysts developed from oocytes matured in vitro with/without melatonin treatment and in vivo, a total of 314 GV oocytes and 320 MI oocytes were collected from 200 patients younger than 35 years old undergoing ICSI cycle. The oocytes were randomly distributed in the control group (no melatonin) and four other groups of varying melatonin concentrations (10‑11, 10‑9, 10‑7, 10‑5 mol/l)...
June 9, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627068/feed-restriction-regime-in-a-rabbit-line-selected-for-growth-rate-alters-oocyte-maturation-manifested-by-alteration-in-msy2-gene-expression
#10
C Naturil-Alfonso, D S Peñaranda, J S Vicente, F Marco-Jiménez
Young rabbit females selected for growth rate may have nutritional needs, which may not be met with the common practice of feed restriction during rearing in commercial rabbit production. The aim of this study was to analyse whether two different feeding programmes: ad libitum or restricted (130 g/day) feeding, applied in young rabbit females for 1 month at the end of rearing, could modulate the origin of ovulation process and the quality of the oocytes. At 16 weeks of age, 34 females were randomly assigned to restricted or ad libitum feeding, maintaining these conditions for a month...
June 18, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28625761/impact-of-vitamin-d-levels-on-ovarian-reserve-and-ovarian-response-to-ovarian-stimulation-in-oocyte-donors
#11
Alberta Maria Fabris, Maria Cruz, Carlos Iglesias, Alberto Pacheco, Azadeh Patel, Jayesh Patel, Human Fatemi, Juan Antonio García-Velasco
Recently, growing interest in vitamin D has emerged from findings that demonstrate a low vitamin D status in populations. Similarly, much interest has been shown in the role that anti-Müllerian hormone (AMH) plays in reproductive physiology. Considerable confusion as to whether vitamin D status is related to ovarian function can be found in the literature. Our retrospective study was performed from June 2014 to April 2015. Oocyte donors were recruited and stimulated under the antagonist protocol with gonadotrophin-releasing hormone (GnRH) agonist to trigger ovulation...
May 19, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#12
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28609268/key-performance-indicators-score-kpis-score-based-on-clinical-and-laboratorial-parameters-can-establish-benchmarks-for-internal-quality-control-in-an-art-program
#13
José G Franco, Claudia G Petersen, Ana L Mauri, Laura D Vagnini, Adriana Renzi, Bruna Petersen, M C Mattila, Vanessa A Comar, Juliana Ricci, Felipe Dieamant, João Batista A Oliveira, Ricardo L R Baruffi
OBJECTIVE: KPIs have been employed for internal quality control (IQC) in ART. However, clinical KPIs (C-KPIs) such as age, AMH and number of oocytes collected are never added to laboratory KPIs (L-KPIs), such as fertilization rate and morphological quality of the embryos for analysis, even though the final endpoint is the evaluation of clinical pregnancy rates. This paper analyzed if a KPIs-score strategy with clinical and laboratorial parameters could be used to establish benchmarks for IQC in ART cycles...
June 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28603764/data-on-endogenous-bovine-ovarian-follicular-cells-peptides-and-small-proteins-obtained-through-top-down-high-resolution-mass-spectrometry
#14
Valérie Labas, Ana-Paula Teixeira-Gomes, Laura Bouguereau, Audrey Gargaros, Lucie Spina, Aurélie Marestaing, Svetlana Uzbekova
The endogenous peptides and small proteins extracted from bovine ovarian follicular cells (oocytes, cumulus and granulosa cells) were identified by Top-down High Resolution Mass Spectrometry (TD-HR-MS/MS) in order to annotate peptido- and proteoforms detected using qualitative and quantitative profiling method based on ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The description and analysis of these Top-down MS data in the context of oocyte quality biomarkers research are available in the original research article of Labas et al...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28600104/polarization-microscopy-imaging-for-the-identification-of-unfertilized-oocytes-after-short-term-insemination
#15
Yi Guo, Wenqiang Liu, Yu Wang, Jiaping Pan, Shanshan Liang, Jingling Ruan, Xiaoming Teng
OBJECTIVE: To develop a unique approach using polarization microscopy (PM) to determine whether the presence of a spindle can be used as an indicator associated with fertilization failure 5 hours after short-term insemination. DESIGN: Observational study. SETTING: Assisted reproduction center. PATIENT(S): Eighty-five patients undergoing short-term insemination. INTERVENTION(S): Oocytes imaged via PM at 4, 5, and 6 hours after standard insemination...
June 6, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28598500/using-propensity-score-matching-to-evaluate-the-effect-of-complementary-medicine-on-clinical-and-embryologic-outcomes-of-in-vitro-fertilization
#16
Anat Porat-Katz, Talia Eldar-Geva, Arik Kahane, Ora Paltiel
OBJECTIVE: To evaluate associations between using complementary medical therapies (CMTs) and embryologic and clinical outcomes of in vitro fertilization (IVF). METHODS: The present prospective questionnaire-based cohort study enrolled women aged 18-44 years undergoing their first, second, or third IVF cycle using their own oocytes at a large IVF clinic in Israel between February 1, 2013, and July 31, 2015. Clinical and embryologic data were obtained from patient records and patients completed a self-administered questionnaire that included details of any CMTs used to treat infertility...
May 15, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28592334/impact-of-oocyte-secreted-factors-on-its-developmental-competence-in-buffalo
#17
Swati Gupta, Sriti Pandey, Mehtab S Parmar, Anjali Somal, Avishek Paul, Bibhudatta S K Panda, Irfan A Bhat, Indu Baiju, Mukesh K Bharti, G Saikumar, Mihir Sarkar, Vikash Chandra, G Taru Sharma
Oocyte-secreted factors (OSFs) play an important role in the acquisition of oocyte developmental competence through bidirectional cross-talk between oocyte and cumulus cells via gap junctions. Thus, the present study was designed to investigate the effect of two OSFs, growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), on the developmental competence of buffalo oocytes derived from two different follicle sizes. Cumulus-oocyte complexes (COCs) from large follicles (LF, >6 mm) or small follicles (SF, 0...
June 8, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28583607/birth-of-healthy-calves-after-intra-follicular-transfer-ifot-of-slaughterhouse-derived-immature-bovine-oocytes
#18
Hoelker Michael, Kassens Ana, Salilew-Wondim Dessie, Sieme Harald, Wrenzycki Christine, Tesfaye Dawit, Neuhoff Christiane, Schellander Karl, Held-Hoelker Eva
To circumvent the negative impacts of in vitro culture on bovine embryos, we have recently established a new method, the so called intra-follicular oocyte transfer (IFOT), enabling in vivo fertilization and in vivo development of in vitro matured oocytes up to the blastocyst stage as well as to term. In this study, we raised the question whether immature bovine oocytes could also be transferred into a pre-ovulatory follicle to support in vivo maturation prior to subsequent in vivo fertilization, in vivo development as well as to term...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583595/the-copper-transporter-slc31a1-ctr1-is-expressed-in-bovine-spermatozoa-and-oocytes-copper-in-ivf-medium-improves-sperm-quality
#19
J P Anchordoquy, J M Anchordoquy, A M Pascua, N Nikoloff, P Peral-García, C C Furnus
Adequate dietary intake of copper (Cu) is required for normal reproductive performance in cattle. The objective of this study was to investigate the pregnancy rates from cattle with deficient, marginal and adequate Cu plasma concentration at the beginning of artificial insemination protocol. Moreover, we determined Cu concentrations present in bovine oviductal fluid (OF), and the effects of Cu on fertilizing ability of bovine spermatozoa. Also, the presence of Cu transporter, SLC31A1 (also known as CTR1), in spermatozoa and in vitro matured oocyte were investigated...
July 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28583421/gene-expression-profiles-in-mouse-cumulus-cells-derived-from-in%C3%A2-vitro-matured-oocytes-with-and-without-blastocyst-formation
#20
Yixin Xu, Tuanping Zhou, Li Shao, Bei Zhang, Kailu Liu, Chao Gao, Li Gao, Jiayin Liu, Yugui Cui, Ri-Cheng Chian
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertilization (IVF). In our study, the corresponding mouse oocytes were traced using a single-cell tracking system, and CCs were pooled into groups based on the embryonic developmental outcomes...
June 2, 2017: Gene Expression Patterns: GEP
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