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https://www.readbyqxmd.com/read/29352731/induced-cumulus-expansion-of-poor-quality-buffalo-cumulus-oocyte-complexes-by-interleukin-1beta-improves-their-developmental-ability
#1
Gaurav Kumar Chaubey, Sandeep Kumar, Manish Kumar, Parul Sarwalia, Arumugam Kumaresan, Sachinandan De, Rakesh Kumar, Tirtha Kumar Datta
The present study was conceived with the aim of modulating the cumulus expansion characteristics of poor quality (BCB-) buffalo oocyte complexes (COCs) in order to improve their fertilization outcomes. BCB- COCs were subjected to in vitro maturation (IVM) in presence of Interleukin - 1 beta (IL-1β) along with BCB- (control) and good quality (BCB +) COCs. Results were assessed morphologically, by scanning electron microscopy (SEM) and by expression analysis of cumulus expansion related genes. Also, numbers of zona pellucida bound spermatozoa were counted and development rates of oocytes were monitored under different groups...
January 20, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29345445/the-impact-of-high-progesterone-levels-on-the-day-of-hcg-administration-in-assisted-human-reproduction-treatments
#2
Michelli S Tanada, Ivan H Yoshida, Monise Santos, Caroline Z Berton, Elen Souto, Waldemar P de Carvalho, Emerson B Cordts, Caio P Barbosa
OBJECTIVE: Progesterone is a steroid hormone that acts on the endometrium. It is known for producing physical and mood-related side effects. Few studies have looked into how progesterone levels affect embryo development and quality. This study aimed to find a cutoff level for serum progesterone on the day of HCG administration from which embryo quality is impaired. METHODS: The study included 145 cycles, from which 885 oocytes and 613 embryos were obtained. All patients had their serum progesterone levels measured on the day of HCG administration...
January 18, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29345310/a-potential-role-of-knockout-serum-replacement-as-a-porcine-follicular-fluid-substitute-for-in-vitro-maturation-lipid-metabolism-approach
#3
Jun-Xue Jin, Sanghoon Lee, Erif Maha Nugraha Setyawan, Anukul Taweechaipaisankul, Geon A Kim, Ho Jae Han, Curie Ahn, Byeong Chun Lee
The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. Knockout serum replacement (KSR) is a synthetic protein source, without any undefined growth factors or differentiation-promoting factors. Therefore, it is feasible to use KSR as a defined component for avoiding effects of unknown molecules in an IVM system...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29334199/comparison-of-cabergoline-and-quinagolide-in-prevention-of-severe-ovarian-hyperstimulation-syndrome-among-patients-undergoing-intracytoplasmic-sperm-injection
#4
Robabeh Taheripanah, Mahshid Vasef, Marzieh Zamaniyan, Anahita Taheripanah
Background: The aim of the current study is to compare quinagolide with cabergoline in prevention of ovarian hyperstimulation syndrome (OHSS) among high risk women undergoing intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS: This randomized clinical trial study was performed from March 2015 to February 2017. One hundred and twenty six women undergoing ICSI who were at high risk of developing OHSS (having over 20 follicles of >12 mm), were randomized into two groups...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29334036/short-term-storage-of-the-oocytes-affects-the-ploidy-status-in-the-yellowtail-tetra-astyanax-altiparanae
#5
Matheus Pereira Dos Santos, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, Nycolas Levy Pereira, Takafumi Fujimoto, José Augusto Senhorini, Laura Satiko Okada Nakaghi
In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26°C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage...
January 15, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29333568/comparison-of-effects-of-thawing-entire-donor-sperm-vial-vs-partial-thawing-shaving-on-sperm-quality
#6
Micha Baum, Raoul Orvieto, Sapir Kon, Ronit Machtinger, Gil M Yerushalmi, Ariel Hourvitz
PURPOSE: Partial thawing of a vial of cryopreserved sperm (shaving) is sometimes applied as a measure to preserve sperm for further use, particularly in cases of very restricted sperm quantity. However, mechanical violence may disrupt the sperm-wall and lead to impaired in-vitro fertilization (IVF) outcomes. MATERIAL AND METHODS: In a retrospective case-control study at a tertiary, university-affiliated medical center, we compared the IVF/intracytoplasmic sperm injection (ICSI) outcomes of patients who used donor sperm following partial thawing (shaving) of the vial of cryopreserved sperm (n = 99) to a control group consisting of patients for whom the vial of sperm was completely thawed before use (n = 99)...
January 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29331044/journey-of-oocyte-from-metaphase-i-to-metaphase-ii-stage-in-mammals
#7
Alka Sharma, Meenakshi Tiwari, Anumegha Gupta, Ashutosh N Pandey, Pramod K Yadav, Shail K Chaube
In mammals, journey from metaphase-I (M-I) to metaphase-II (M-II) is important since oocyte extrude first polar body (PB-I) and gets converted into haploid gamete. The molecular and cellular changes associated with meiotic cell cycle progression from M-I to M-II stage and extrusion of PB-I remain ill understood. Several factors drive oocyte meiosis from M-I to M-II stage. The mitogen-activated protein kinase3/1 (MAPK3/1), signal molecules and Rho family GTPases act through various pathways to drive cell cycle progression from M-I to M-II stage...
January 13, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29324379/associating-ambient-exposure-to-fine-particles-and-human-fertility-rates-in-china
#8
Tao Xue, Qiang Zhang
BACKGROUND: Adverse effects of ambient fine particles (PM2.5) on sperm quality and oocyte fertilization have been identified by previous research. However, insufficient human studies tested associations between PM2.5 and decreased fertility rates. METHODS: We associated long-term exposure to PM2.5 and county-level fertility rates reported by 2010 census across China. Exposure assessments were based on PM2.5 maps (2009-2010) with a spatial resolution of 0.1° derived from satellite remote sensing data from another published study...
January 8, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29305844/progesterone-rise-on-hcg-day-is-negatively-correlated-with-ivf-et-outcomes-in-natural-cycles
#9
Xiadi Wu, Yundong Mao, Yan Gao, Xiaoqiao Qian, Wei Wang, Wei Ding, Shi-Wen Jiang, Jiayin Liu
OBJECTIVE: To investigate the effect of progesterone rise on hCG day on the laboratory and clinical outcomes in natural cycles and to explore the possible factors related to the occurrence of progesterone elevation. PATIENTS AND STUDY METHODS: Retrospective analysis was performed in 1157 infertile women with decreased ovarian reserve. Eligible infertile women undergoing IVF in natural cycles were assigned to four groups according to serum progesterone levels on the day of hCG administration: group 1, P<2nmol/L; group 2, 2<P<2...
January 3, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29303233/high-percentages-of-embryos-with-21-18-or-13-trisomy-are-related-to-advanced-paternal-age-in-donor-egg-cycles
#10
Javier García-Ferreyra, Roly Hilario, Julio Dueñas
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos)...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29298334/increased-fitness-of-a-key-appendicularian-zooplankton-species-under-warmer-acidified-seawater-conditions
#11
Jean-Marie Bouquet, Christofer Troedsson, Aliona Novac, Magnus Reeve, Anna K Lechtenbörger, Wendy Massart, Katrine S Skaar, Anne Aasjord, Sam Dupont, Eric M Thompson
Ocean warming and acidification (OA) may alter the fitness of species in marine pelagic ecosystems through community effects or direct physiological impacts. We used the zooplanktonic appendicularian, Oikopleura dioica, to assess temperature and pH effects at mesocosm and microcosm scales. In mesocosms, both OA and warming positively impacted O. dioica abundance over successive generations. In microcosms, the positive impact of OA, was observed to result from increased fecundity. In contrast, increased pH, observed for example during phytoplankton blooms, reduced fecundity...
2018: PloS One
https://www.readbyqxmd.com/read/29288975/fertilizing-capacity-of-vitrified-epididymal-sperm-from-iberian-ibex-capra-pyrenaica
#12
J Pradieé, M J Sánchez-Calabuig, C Castaño, E O'Brien, M C Esteso, P Beltrán-Breña, V Maillo, J Santiago-Moreno, D Rizos
In this study, we successfully described for the first time a vitrification of epididymal Iberian ibex spermatozoa. Spermatozoa from epididymis were obtained from 15 Iberian ibex. The right epididymis' semen sample was vitrified and the left one was frozen. After thawing/warming, samples were selected by density gradient. Sperm characteristics from each treatment were evaluated. To test the spermatozoa fertilization ability, heterologous IVF was carried out using bovine oocytes. Despite of the observation of a decrease of about 40% for motility sperm between pre-freezing and post-thawing (75...
December 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29285797/sodium-fluoride-disturbs-dna-methylation-of-nnat-and-declines-oocyte-quality-by-impairing-glucose-transport-in-porcine-oocytes
#13
Xiaoyan Liu, Zheng-Wen Nie, Ying-Ying Gao, Li Chen, Shu-Yuan Yin, Xia Zhang, Cuifang Hao, Yi-Liang Miao
Sodium fluoride (NaF) is used as a medicine to prevent tooth decay; however, excessive NaF could cause a pathological damage to the health. Recent studies showed that NaF impaired mouse oocyte maturation, included of abnormal spindle configuration, actin cap formation, cortical granule-free domain formation, and the following development after fertilization. However, few studies used large animals as models to study the toxicology of NaF on oocytes maturation. We proposed a hypothesis that NaF would affect the nuclear and cytoplasmic maturation of porcine oocytes and DNA methylation pattern of imprinted genes in oocytes...
December 29, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29283885/transfer-of-autologous-mitochondria-from-adipose-tissue-derived-stem-cells-rescues-oocyte-quality-and-infertility-in-aged-mice
#14
Zhen-Bo Wang, Jian-Xiu Hao, Tie-Gang Meng, Lei Guo, Ming-Zhe Dong, Li-Hua Fan, Ying-Chun Ouyang, Guopeng Wang, Qing-Yuan Sun, Xiang-Hong Ou, Yuan-Qing Yao
Elder women suffer from low or loss of fertility because of decreasing oocyte quality as maternal aging. As energy resource, mitochondria play pivotal roles in oocyte development, determining oocyte quality. With advanced maternal age, increased dysfunctions emerge in oocyte mitochondria, which decrease oocyte quality and its developmental potential. Mitochondria supplement as a possible strategy for improving egg quality has been in debate due to ethnic problems. Heterogeneity is an intractable problem even transfer of germinal vesicle, spindle, pronuclei or polar body is employed...
December 27, 2017: Aging
https://www.readbyqxmd.com/read/29282414/level-of-vitamin-e-in-follicular-fluid-and-serum-and-oocyte-morphology-and-embryo-quality-in-patients-undergoing-ivf-treatment
#15
Mohammad Hadi Bahadori, Seyedeh Hajar Sharami, Fereshteh Fakor, Forozan Milani, Davoud Pourmarzi, Seyedeh Fatemeh Dalil-Heirati
Objective: This study aimed to determine the relationship between vitamin E in the follicular fluid (FF) and serum with oocyte morphology and embryo quality. Materials and methods: A cross-sectional study was conducted on serum samples, FF samples, oocytes, and embryos collected from 50 women undergoing in vitro fertilization in the Alzahra Hospital, Rasht, Iran from March to August 2014. Vitamin E level was measured using HPLC. Oocyte morphology and embryo quality were evaluated during inverted optical microscopy...
June 2017: Journal of Family & Reproductive Health
https://www.readbyqxmd.com/read/29281695/maternal-metabolism-affects-endometrial-expression-of-oxidative-stress-and-foxl2-genes-in-cattle
#16
Audrey Lesage-Padilla, Niamh Forde, Mélanie Poirée, Gareth D Healey, Corinne Giraud-Delville, Pierrette Reinaud, Caroline Eozenou, Anaïs Vitorino Carvalho, Laurent Galio, Mariam Raliou, Jean-François Oudin, Christophe Richard, I Martin Sheldon, Gilles Charpigny, Pat Lonergan, Olivier Sandra
Intensive selection for milk production has led to reduced reproductive efficiency in high-producing dairy cattle. The impact of intensive milk production on oocyte quality as well as early embryo development has been established but few analyses have addressed this question at the initiation of implantation, a critical milestone ensuring a successful pregnancy and normal post-natal development. Our study aimed to determine if contrasted maternal metabolism affects the previously described sensory properties of the endometrium to the conceptus in cattle...
2017: PloS One
https://www.readbyqxmd.com/read/29278228/an-in-vitro-study-on-oocyte-and-follicles-of-transplanted-ovaries-treated-with-vascular-endothelial-growth-factor
#17
Maryam Zand-Vakili, Afsaneh Golkar-Narenji, Paul E Mozdziak, Hussein Eimani
OBJECTIVE: Retrieval of high quality follicles and oocytes from transplanted ovaries is essential for higher fertility preservation efficiency. The effect of vascular endothelial growth factor (VEGF) was evaluated on the survival rate of preantral follicles following ovarian transplantation. MATERIAL AND METHODS: Prepubertal female mice were divided to 6 groups including: control (C), transplanted with no VEGF treatment (T) and transplanted with different dosages of VEGF [0...
December 15, 2017: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/29260383/oxidative-stress-in-granulosa-cells-contributes-to-poor-oocyte-quality-and-ivf-et-outcomes-in-women-with-polycystic-ovary-syndrome
#18
Qiaohong Lai, Wenpei Xiang, Qing Li, Hanwang Zhang, Yufeng Li, Guijin Zhu, Chengliang Xiong, Lei Jin
The increased levels of intracellular reactive oxygen species (ROS) in granulosa cells (GCs) may affect the pregnancy results in women with polycystic ovary syndrome (PCOS). In this study, we compared the in vitro fertilization and embryo transfer (IVF-ET) results of 22 patients with PCOS and 25 patients with tubal factor infertility and detected the ROS levels in the GCs of these two groups. Results showed that the PCOS group had significantly larger follicles on the administration day for human chorionic gonadotropin than the tubal factor group (P < 0...
December 19, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/29259463/pregnancy-outcomes-after-assisted-reproductive-procedures-with-embryos-that-had-been-derived-from-affected-and-unaffected-ovaries-among-women-with-small-unilateral-endometriomas
#19
Akiko Takashima, Naoki Takeshita, Toshihiko Kinoshita
Aim: To clarify the effects of small endometriomas on in vitro fertilization (IVF) outcomes. In the present study, the potential impact of small ovarian endometriomas on the quantitative and qualitative outcomes of IVF was evaluated in the same individual. Methods: A retrospective analysis was performed, in which 118 infertile women with unilateral endometriomas that were <40 mm in size and who underwent IVF or intracytoplasmic sperm injection were evaluated...
April 2017: Reproductive Medicine and Biology
https://www.readbyqxmd.com/read/29259447/equilibrium-between-anti-oxidants-and-reactive-oxygen-species-a-requisite-for-oocyte-development-and-maturation
#20
REVIEW
Manika Kala, Muhammad Vaseem Shaikh, Manish Nivsarkar
Reactive oxygen species (ROS) are required for cellular functioning and are controlled by anti-oxidants. The ROS influence the follicles, oocytes, endometrium, and their environment. The luteinizing hormone surge initiates a massive recruitment of ROS that modulates major reproductive functions namely, oocyte maturation, ovarian steroidogenesis, corpus luteal function, and luteolysis. The anti-oxidant system balances ROS generation and maintains the cellular functions. Both enzymatic and non-enzymatic anti-oxidants namely, vitamins and minerals are present in the follicles and protect the oocytes from the damaging effects of ROS...
January 2017: Reproductive Medicine and Biology
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