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https://www.readbyqxmd.com/read/28093041/blastocyst-development-in-a-single-medium-compared-to-sequential-media
#1
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, George K Petsas, Basil C Tarlatzis, Trifon G Lainas
The aim of the present study was to compare blastocyst formation rates after embryo culture in a single medium (Global) as compared to sequential media (ISM1/BlastAssist). In this prospective trial with sibling oocytes, 542 metaphase II (MII) oocytes from 31 women were randomly and equally divided to be fertilized and cultured to the blastocyst stage in either sequential media (ISM1/BlastAssist; n = 271 MII oocytes) or a single medium (Global; n = 271 MII oocytes). In both groups, embryos were cultured in an interrupted fashion with media changes on day 3...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28089824/mitochondrial-uncoupling-protein-2-in-human-cumulus-cells-is-associated-with-regulating-autophagy-and-apoptosis-maintaining-gap-junction-integrity-and-progesterone-synthesis
#2
Hongshan Ge, Fan Zhang, Duan Ping, Nan Zhu, Jiayan Zhang, Feijun Ye, Dan Shan, Hua Chen, XiaoSheng Lu, ChunFang Zhu, Renshan Ge, Zhenkun Lin
To explore the roles of mitochondrial Uncoupling Protein 2 (UCP2) in cumulus cells (CCs), human CCs were cultured in vitro, and the UCP2 was inhibited by treatment with Genipin, a special UCP inhibitor, or by RNA interference targeting UCP2. No significant differences in adenosine triphosphate levels and the ratio of ADP/ATP were observed after UCP2 inhibition. UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG...
January 12, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28089717/persistent-organic-pollutants-pops-in-human-follicular-fluid-and-in-vitro-fertilization-outcomes-a-pilot-study
#3
Michael S Bloom, Victor Y Fujimoto, Robin Storm, Li Zhang, Celeste D Butts, Diana Sollohub, Robert L Jansing
Persistent organic pollutants (POPs) are ubiquitously distributed among the U.S. population and adversely impact human reproduction. These compounds have been detected in human ovarian follicular fluid (FF), where they directly contact a developing oocyte. As a pilot investigation, we measured 43 polychlorinated biphenyl (PCB) congeners, p,p'-dichlorodiphenyltrichloroethane (DDT), and its persistent metabolite p,p'-dichlorodiphenyldichloroethylene (DDE) in residual FF collected from 32 women undergoing in vitro fertilization (IVF)...
January 9, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28087314/a-protective-role-of-cumulus-cells-after-short-term-exposure-of-rat-cumulus-cell-oocyte-complexes-to-lifestyle-or-environmental-contaminants
#4
Kelly A Campen, Kenneth P McNatty, Janet L Pitman
Ovarian follicular fluid provides a potential reservoir for exogenous compounds that may adversely affect oocyte quality. This study examined the effects of common lifestyle and environmental contaminants, namely bisphenol-A (BPA), caffeine, 3,4-methylenedioxymethamphetamine (MDMA), nicotine and Δ(9)-tetrahydrocannabinol on gap junction genes (Gja1, Gja4) and proteins (GJA1), glucose metabolism genes (Gfpt1, Pfkp) and oocyte growth factor genes (Bmp15, Gdf9), as well as gap junction transfer rate, in rat cumulus-oocyte complexes (COCs)...
January 10, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28077604/the-mitochondrial-dna-content-of-cumulus-granulosa-cells-is-linked-to-embryo-quality
#5
V Desquiret-Dumas, A Clément, V Seegers, L Boucret, V Ferré-L'Hotellier, P E Bouet, P Descamps, V Procaccio, P Reynier, P May-Panloup
STUDY QUESTION: Could the mitochondrial DNA (mtDNA) content of cumulus granulosa cells (CGCs) be related to oocyte competence? SUMMARY ANSWER: The quality of embryos obtained during IVF procedures appears to be linked to mtDNA copy numbers in the CGCs. WHAT IS KNOWN ALREADY: Oocyte quality is linked to oocyte mtDNA content in the human and other species, and the mtDNA copy number of the oocyte is related to that of the corresponding CGCs. Moreover, the quantification of CGC mtDNA has recently been proposed as a biomarker of embryo viability...
January 6, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28076980/status-of-sperm-born-oocyte-activating-factors-pawp-plc%C3%AE-and-sperm-chromatin-in-uncapacitated-capacitated-and-acrosome-reacted-conditions
#6
Marziyeh Tavalaee, Kazem Parivar, Abdol-Hossein Shahverdi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
The aim of this study was to investigate the value of sperm factors PAWP and PLCζ involved in oocyte activation and sperm chromatin status, as indicators of sperm quality during uncapacitated, capacitated and acrosome-reacted conditions. Semen samples collected from 10 normozoospermic men and sperm factors PAWP, PLCζ, protamine deficiency and DNA fragmentation evaluated in fresh samples, processed sample by DGC (density gradient centrifugation), capacitated and acrosome reacted sperm, using immunofluorescence and flow cytometry...
January 12, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28073982/urinary-triclosan-concentrations-and-early-outcomes-of-in-vitro-fertilization-embryo-transfer
#7
Rui Hua, Yao Zhou, Biao Wu, Zhongwei Huang, Yongtong Zhu, Yali Song, Yanhong Yu, Hong Li, Song Quan
Triclosan (TCS) exists ubiquitously in the environment. Several in vitro and in vivo studies have demonstrated that TCS exerts endocrine disruptive effects on reproduction, but data from human populations are limited and conflicting. The objective of our study was to investigate whether high urinary TCS concentration is adversely associated with early reproductive outcomes in women undergoing in vitro fertilization-embryo transfer (IVF-ET). This prospective cohort study was conducted from September 2015 to June 2016, including 156 infertile women undergoing their first IVF-ET cycle...
March 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28069903/improvement-of-in-vitro-produced-bovine-embryo-treated-with-coa-a-under-heat-stressed-condition
#8
Imran Khan, Kyeong-Lim Lee, Lianguang Xu, Ayman Mesalam, Mmr Chowdhury, Myeong-Don Joo, Ihsan-Ul- Haq, Bushra Mirza, Il-Keun Kong
Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 °C and 42 °C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 °C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kB) and cyclooxygenase-2 (COX2)...
January 9, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28066836/a-comparative-study-on-the-results-of-agonist-and-antagonist-protocols-based-on-serum-amh-levels-in-patients-undergoing-intracytoplasmic-sperm-injection
#9
Fatemeh Nikmard, Behrouz Aflatoonian, Elham Hosseini, Abbas Aflatoonian, Mehrdad Bakhtiyari, Reza Aflatoonian
BACKGROUND: Serum concentrations of antimullerian hormone (AMH) correlate with ovarian response during assisted reproduction treatment (ART) cycles. OBJECTIVE: This retrospective study attempted to evaluate the selection of ovarian stimulation protocols based on serum AMH levels in patients and its impact on the results of ART. MATERIALS AND METHODS: Based on AMH levels, the patients with tubal factor infertility were divided in three groups of normal, low and high AMH levels...
December 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28066833/the-role-of-g-csf-in-recurrent-implantation-failure-a-randomized-double-blind-placebo-control-trial
#10
Fatemeh Davari-Tanha, Ensieh Shahrokh Tehraninejad, Mohadese Ghazi, Zahra Shahraki
BACKGROUND: Recurrent implantation failure (RIF) is the absence of implantation after three consecutive In Vitro Fertilization (IVF) cycles with transferring at least four good quality embryos in a minimum of three fresh or frozen cycles in a woman under 40 years. The definition and management of RIF is under constant scrutiny. OBJECTIVE: To investigate the effects of Granulocyte colony stimulating factor (G-CSF) on RIF, pregnancy rate, abortion rate and implantation rates...
December 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28063063/identifying-candidate-reprogramming-genes-in-mouse-induced-pluripotent-stem-cells
#11
Fang Gao, Jingyu Li, Heng Zhang, Xu Yang, Tiezhu An
Factor-based induced reprogramming approaches have tremendous potential for human regenerative medicine, but the efficiencies of these approaches are still low. In this study, we analyzed the global transcriptional profiles of mouse induced pluripotent stem cells (miPSCs) and mouse embryonic stem cells (mESCs) from seven different labs and present here the first successful clustering according to cell type, not by lab of origin. We identified 2131 different expression genes (DEs) as candidate pluripotency-associated genes by comparing mESCs/miPSCs with somatic cells and 720 DEs between miPSCs and mESCs...
January 6, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28062641/melatonin-levels-in-follicular-fluid-as-markers-for-ivf-outcomes-and-predicting-ovarian-reserve
#12
Jing Tong, Shi-le Sheng, Yun Sun, Huihui Li, Wei-Ping Li, Cong Zhang, Zijiang Chen
Good-quality oocytes are critical for the success of in vitro fertilization (IVF), but, to date, there is no marker of ovarian reserve available that can accurately predict oocyte quality. Melatonin exerts its antioxidant actions as a strong radical scavenger that might affect oocyte quality directly, since it is the most potent antioxidant in follicular fluid. To investigate the precise role of endogenous melatonin in IVF outcomes, we recruited 61 women undergoing treatment cycles of IVF or intracytoplasmic sperm injection (ICSI) procedures and classified them into three groups according to their response to ovarian stimulation...
January 6, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#13
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28056063/comparative-study-of-reproductive-development-in-wild-and-captive-reared-greater-amberjack-seriola-dumerili-risso-1810
#14
Rosa Zupa, Covadonga Rodríguez, Constantinos C Mylonas, Hanna Rosenfeld, Ioannis Fakriadis, Maria Papadaki, José A Pérez, Chrysovalentinos Pousis, Gualtiero Basilone, Aldo Corriero
The greater amberjack Seriola dumerili is a large teleost fish with rapid growth and excellent flesh quality, whose domestication represents an ambitious challenge for aquaculture. The occurrence of reproductive dysfunctions in greater amberjack reared in captivity was investigated by comparing reproductive development of wild and captive-reared individuals. Wild and captive-reared breeders were sampled in the Mediterranean Sea during three different phases of the reproductive cycle: early gametogenesis (EARLY, late April-early May), advanced gametogenesis (ADVANCED, late May-early June) and spawning (SPAWNING, late June-July)...
2017: PloS One
https://www.readbyqxmd.com/read/28051267/reduced-expression-of-scf-in-serum-and-follicle-from-patients-with-polycystic-ovary-syndrome
#15
J Tan, X-Y Wen, Q Su, Z-H Huang, J-X He, C-L Xin, Q-F Wu, Y Zou
OBJECTIVE: In humans, stem cell factor (SCF), produced by cumulus granulosa cells during the follicular phase, plays a crucial role in follicular development. Remarkably, polycystic ovary syndrome (PCOS), one of the main reasons affecting women fertility, is accompanied by some abnormal follicles. Is there a relationship between SCF and PCOS? This study aimed to compare the expression of SCF in follicle and serum from patients with and without PCOS undergoing in vitro fertilization (IVF) treatment and to investigate the potential relationship between aberrant SCF expression and PCOS...
December 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28051220/the-influence-of-gonadotropins-on-clinico-biological-icsi-outcome-a-retrospective-comparative-study-rfsh-vs-hp-hmg
#16
Abdeljalil Khlifi, Olfa Kacem, Maher Maroueni, Leila Elgoul, Samir Hidar, Meriem Fekih, Sassi Boughizane, Habib Essaidi, Lassad Ben Regaya, Mohamed Bibi, Mounir Ajina, Hedi Khairi
Objective To investigate the difference in the outcome of ICSI-ET cycles among respondents patients, taking into account the molecule inducer of controlled ovarian stimulation: HP-hMG ou rFSH. Patients and Methods A comparative retrospective study over 62 months including a total of 1005 infertile couples, divided into two groups: HP-HMG (n=125) and rFSH (n=880). Results - The average numbers of retrieved oocytes and matures oocytes were significantly higher in rFSH group rFSH (7,94 ± 2,49, HP-HMG vs 9,05 ± 3,40, rFSH, p=0...
June 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/28050957/prediction-of-metaphase-ii-oocytes-according-to-different-serum-anti-m%C3%A3-llerian-hormone-amh-levels-in-antagonist-icsi-cycles
#17
Joyce B da Silva, Tatiana R Panaino, Maria A Tamm, Paloma Lira, Patricia C F Arêas, Ana C A Mancebo, Marcelo M de Souza, Roberto A Antunes, Maria do Carmo B de Souza
OBJECTIVE: This paper aims to assess a qualitative aspect of ovarian response in terms of metaphase II oocytes according to different serum Anti-Müllerian hormone levels in antagonist ICSI cycles. A prediction index might contribute to the individualization of care. METHODS: This observational study looked into 287 antagonist ICSI cycles carried out with patients treated in a single center between January of 2012 and January of 2016. Serum AMH and subgroup analyses were performed based on five AMH ranges (≤ 0...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28045561/age-related-changes-in-gene-expression-patterns-of-immature-and-aged-rat-primordial-follicles
#18
Vijayakumar Govindaraj, Harshini Krishnagiri, Payal Chakraborty, Madavan Vasudevan, A Jagannadha Rao
Women are born with millions of primordial follicles which gradually decrease with increasing age and this irreversible supply of follicles completely exhausts at menopause. The fertility capacity of women diminishes in parallel with aging. The mechanisms for reproductive aging are not fully understood. We have observed a decline in Brca1 mediated DNA repair in aging rat primordial follicles. To further understand the age-related molecular changes, we performed microarray gene expression analysis using total RNA extracted from immature (18 to 20 day old) and aged (400 to 450 day old) rat primordial follicles...
January 3, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28043373/comparison-of-fertility-following-use-of-one-versus-two-intravaginal-progesterone-inserts-in-dairy-cows-without-a-cl-during-a-synchronization-protocol-before-timed-ai-or-timed-embryo-transfer
#19
M H C Pereira, C P Sanches, T G Guida, M C Wiltbank, J L M Vasconcelos
The objective was to evaluate the effect of increased progesterone (P4) during preovulatory follicle growth during timed AI (TAI) or timed embryo transfer (TET) protocols. Lactating dairy cows with no CL and low circulating P4 (≤1.0 ng/mL) were submitted to a protocol using one or two intravaginal P4 implants (controlled intravaginal releasing device [CIDRs]), and were bred to TAI or TET. The low P4 cows for this experiment were identified on nine farms, four utilized TAI (n = 326 of 1160 cows examined), and five utilized TET (n = 445 of 1396)...
February 2017: Theriogenology
https://www.readbyqxmd.com/read/28043370/assessment-of-the-effect-of-adding-l-carnitine-and-or-resveratrol-to-maturation-medium-before-vitrification-on-in%C3%A2-vitro-matured-calf-oocytes
#20
José Felipe Sprícigo, Roser Morató, Núria Arcarons, Marc Yeste, Margot Alves Dode, Manuel López-Bejar, Teresa Mogas
Cryopreservation may lead bovine oocytes to undergo morphological changes and functional damage due to the high-lipid content in the cytoplasm and the formation of reactive oxygen species. Against this background, the present study aimed to improve the cryotolerance and developmental competence of prepubertal calf oocytes by adding L-carnitine (LC) and/or resveratrol (R) to the IVM medium, as the former is involved in lipid metabolism and both are able to scavenge reactive oxygen species. With this purpose, different quality and functional oocyte parameters, such as spindle and chromosome configuration, DNA integrity, caspase activity, and the profile of genes involved in lipid metabolism and oxidative stress were evaluated in IVM bovine oocytes before or after vitrification/warming...
February 2017: Theriogenology
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