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https://www.readbyqxmd.com/read/28449121/tri-directional-anaphases-as-a-novel-chromosome-segregation-defect-in-human-oocytes
#1
Jenna Haverfield, Nicola L Dean, Diana Nöel, Gaudeline Rémillard-Labrosse, Veronique Paradis, Isaac-Jacques Kadoch, Greg FitzHarris
STUDY QUESTION: What are the chromosome segregation errors in human oocyte meiosis-I that may underlie oocyte aneuploidy? SUMMARY ANSWER: Multiple modes of chromosome segregation error were observed, including tri-directional anaphases, which we attribute to loss of bipolar spindle structure at anaphase-I. WHAT IS KNOWN ALREADY: Oocyte aneuploidy is common and associated with infertility, but mechanistic information on the chromosome segregation errors underlying these defects is scarce...
April 25, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28447502/the-effect-of-endometrial-injury-on-implantation-and-clinical-pregnancy-rates
#2
Dan Levin, Joseph Hasson, Aviad Cohen, Yuval Or, Baris Ata, Lilia Barzilay, Benny Almog
AIM: To assess the effect of endometrial scratching (ES) on in vitro fertilization-embryo transfer outcome (IVF-ET) Materials and methods: Retrospective matched case control study including all fresh IVF cycles performed between January 2006 and December 2012 at an academic IVF center. ES with an endometrial biopsy catheter was performed in the cycle preceding the index IVF cycle. Patients (n = 238) were pair matched with controls according to age, number of previous failed IVF cycles and number of embryos transferred...
April 27, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28442024/intracytoplasmic-oxidative-stress-reverses-epigenetic-modifications-in-polycystic-ovary-syndrome
#3
Fatemeh Eini, Marefat Ghaffari Novin, Khojasteh Joharchi, Ahmad Hosseini, Hamid Nazarian, Abbas Piryaei, Arash Bidadkosh
In polycystic ovary syndrome (PCOS), substantial genetic and environmental alterations, along with hyperandrogenism, affect the quality of oocytes and decrease ovulation rates. To determine the mechanisms underlying these alterations caused specifically by an increase in plasma androgens, the present study was performed in experimentally-induced PCOS mice. As the study model, female B6D2F1 mice were treated with dehydroepiandrosterone (DHEA, 6mg per 100g bodyweight). After 20 days, oocytes at the germinal vesicle and metaphase II stages were retrieved from isolated ovaries and subsequent analyses of oocyte quality were performed for each mouse...
April 26, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28440264/use-of-testicular-sperm-for-intracytoplasmic-sperm-injection-in-men-with-high-sperm-dna-fragmentation-a-swot-analysis
#4
Sandro C Esteves, Matheus Roque, Nicolás Garrido
Spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation (SDF) in the neat semen tend to have better DNA quality. Given the negative impact of SDF on the outcomes of Assisted Reproductive Technology (ART), an increased interest has emerged about the use of testicular sperm for intracytoplasmic sperm injection (Testi-ICSI). In this article, we used a SWOT (strengths, weaknesses, opportunities, and threats) analysis to summarize the advantages and drawbacks of this intervention...
April 18, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28435188/effect-of-kisspeptin-on-in-vitro-maturation-of-sheep-oocytes
#5
Priyanka Byri, Arunakumari Gangineni, K Ramachandra Reddy, K B P Raghavender
AIM: The aim of this study was to investigate the effect of kisspeptin (KP) on in vitro maturation (IVM) of sheep oocytes aspirated from the ovaries collected from slaughterhouse. MATERIALS AND METHODS: Two different experiments were conducted to investigate the effect of KP (5, 10 and 15 µg/ml) alone (experiment 1) or in combination with follicle-stimulating hormone (FSH), luteinizing hormone (LH), and Estradiol (E2) (experiment 2) on IVM of sheep oocytes. Tissue culture medium 199 supplemented with Gentamicin was used as control medium...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28432827/parenting-stress-and-its-association-with-perceived-agreement-about-the-disclosure-decision-in-parents-following-donor-conception
#6
Anja J Gebhardt, Gunilla Sydsjö, Agneta Skoog Svanberg, Astrid Indekeu, Claudia Lampic
For many donor-conceiving heterosexual parents, the process of deciding whether and what to tell children about their genetic origin is challenging. We hypothesized that incomplete couple agreement about disclosure could be associated with parenting stress. The aim of the study was to investigate (1) parenting stress levels among heterosexual parents of young children following gamete donation and (2) whether parenting stress is related to perceived agreement about disclosure of the donor conception to the children MATERIAL AND METHODS: This study is part of the longitudinal multicenter Swedish Study on Gamete Donation and included a total of 213 heterosexual parents with children age 1-4 years following oocyte donation (n = 103) and sperm donation (n = 110)...
April 22, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28431155/motoring-through-the-role-of-kinesin-superfamily-proteins-in-female-meiosis
#7
Nicole J Camlin, Eileen A McLaughlin, Janet E Holt
BACKGROUND: The kinesin motor protein family consists of 14 distinct subclasses and 45 kinesin proteins in humans. A large number of these proteins, or their orthologues, have been shown to possess essential function(s) in both the mitotic and the meiotic cell cycle. Kinesins have important roles in chromosome separation, microtubule dynamics, spindle formation, cytokinesis and cell cycle progression. This article contains a review of the literature with respect to the role of kinesin motor proteins in female meiosis in model species...
April 18, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28429340/controlled-prospective-observational-study-on-the-efficiency-and-tolerability-of-a-combination-of-potential-nrf2-inducing-antioxidants-and-micronutrients-as-pre-treatment-for-icsi-in-dyspermic-patients-with-previous-failure
#8
R Rago, M Gallo, A Dal Lago, E Licata, G Paciotti, M Amodei, C Meneghini, C Fabiani, G Dani, C Liberanome, D Antonaci, R Corno, D Miriello, G Giuffrida, B Giammusso
OBJECTIVE: To evaluate the efficiency of pre-treatment in dyspermic males in IVF couples with a combination of micronutrients, for the purpose of improving the fertilization rate, the implantation rate and the outcome of the pregnancy. PATIENTS AND METHODS: This controlled prospective clinical study was performed in two medically assisted reproduction centers. 59 males with mild oligo-astheno-teratospermia (OAT) were admitted to the study. All of them had a history of previous in vitro fertilization (IVF) attempts with female partners aged < 40 diagnosed having tubal or idiopathic infertility...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28429153/relative-abundance-of-heat-shock-proteins-and-clusterin-transcripts-in-spermatozoa-collected-form-boar-routinely-utilised-in-an-artificial-insemination-centre-preliminary-results
#9
A Zannoni, C Bernardini, A Zaniboni, E Ferlizza, D Ventrella, M L Bacci, M Forni
It is widely accepted that mature sperm contains RNA. The first hypothesis was that sperm RNAs have no functions of their own but are simply residues of spermatogenesis reflecting the events that occurred during their formation in the testes. More recently new discoveries have essentially expanded these views, showing that sperm mRNAs constitute a population of stable full-length transcripts, many of which are selectively retained during spermatogenesis and delivered to oocytes contributing to early embryo development...
April 21, 2017: Veterinary Research Communications
https://www.readbyqxmd.com/read/28428945/a-successful-pregnancy-using-completely-immotile-but-viable-frozen-thawed-spermatozoa-selected-by-laser
#10
Huanhua Chen, Guixue Feng, Bo Zhang, Hong Zhou, Jinhui Shu, Xianyou Gan
The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39...
March 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28428123/transvaginal-ultrasound-guided-embryo-transfer-in-ivf
#11
Lionel Larue, Gwenola Keromnes, Anne Massari, Clémence Roche, Julie Moulin, Héloïse Gronier, Dominique Bouret, Nino Guy Cassuto, Jean Philippe Ayel
OBJECTIVES: to determine whether transvaginal ultrasound-guided embryo transfer is a technique that can be used routinely, whether it improves IVF outcomes and whether it makes difficult transfers easier and more successful. MATERIAL AND METHOD: non-randomised retrospective study conducted between 2012 and 2016 in the Fertility Centre of the Diaconesses-Croix St Simon Hospital Group. The outcomes of 3910 transfers performed by 5 senior operators under transabdominal ultrasound guidance are compared with those of 800 transfers performed by 1 senior operator under transvaginal ultrasound guidance...
April 17, 2017: J Gynecol Obstet Hum Reprod
https://www.readbyqxmd.com/read/28421902/the-role-of-ovarian-reserve-markers-in-prediction-of-clinical-pregnancy
#12
Ali G Zebitay, Orkun Cetin, Fatma F Verit, Seda Keskin, M Nafi Sakar, Sercin Karahuseyinoglu, Gulsah Ilhan, Sezai Sahmay
To evaluate the role of ovarian reserve markers in the prediction of clinical pregnancy and embryo transfer accomplishment among poor responder IVF applicants. 304 female poor responder IVF applicants were included in this prospective cohort study conducted at the IVF-unit. Antral follicle count, FSH, LH, E2, AMH and IVF outcomes were compared in pregnant and non-pregnant groups as well as in ET vs. non-ET groups. The number of retrieved oocytes was significantly correlated positively with AMH and AFC, and negatively with FSH and age...
May 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28421498/chapter-14-establishing-an-oocyte-cryobank-network
#13
James Graham, Josh Lim, Michael Tucker
Improvements in oocyte cryopreservation has prompted wider acceptance of this technology leading to its use for several reasons. Notably, in addition to elective and medically driven reasons for oocyte cryostorage for fertility preservation, donor oocyte cryobanking is beginning to gain traction, potentially replacing fresh oocyte donation in assisted reproduction. Donor "egg banking," while not totally analogous to donor sperm banking, does provide strong benefits in terms of scheduling flexibility and improved clinical efficiencies, while providing a wider immediate inventory choice...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28420503/age-is-a-major-prognosticator-in-extremely-low-oocyte-retrieval-cycles
#14
Yu-Ting Su, Pin-Yao Lin, Fu-Jen Huang, Fu-Tsai Kung, Yu-Ju Lin, Yi-Ru Tsai, Kuo-Chung Lan
OBJECTIVE: Clinical prognosis appears to be varied in females with poor ovarian response (POR), and poor responders defined by the Bologna criteria might not be sufficiently homogeneous. The aim of this study was to determine the major predictor of reproductive outcomes in extremely low oocyte retrieval cycles. MATERIALS AND METHODS: A cohort of fresh in vitro fertilization/intracytoplasmic sperm injection cycles (n = 858) was analyzed from January 2001 to September 2014...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28420500/does-different-bmi-influence-oocyte-and-embryo-quality-by-inducing-fatty-acid-in-follicular-fluid
#15
Parvaneh Mirabi, Mohammad Javad Chaichi, Sedighe Esmaeilzadeh, Seyed Gholam Ali Jorsaraei, Ali Bijani, Mahjobeh Ehsani
OBJECTIVE: We aimed to assess the effects of obesity-related follicular fluid (FF) fatty acids (FAs) on the number and quality of oocytes, good embryo quality rate, and pregnancy rate. MATERIALS AND METHODS: This prospective cohort study was conducted on 105 infertile women under the age of 38, who underwent intracytoplasmic sperm injection (ICSI) from March 2015 to October 2015. They were grouped into three body mass index (BMI) categories. The fatty acids composition of the FF was analyzed by GC-MS head space method...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28420480/comparative-analysis-of-granulosa-cell-gene-expression-in-association-with-oocyte-competence-in-fsh-stimulated-holstein-cows
#16
David A Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Marc-André Sirard
Ovarian stimulation with exogenous FSH followed by FSH withdrawal or 'coasting' is an effective means of increasing the number of oocytes obtainable for the in vitro production of cattle embryos. However, the quality of the oocytes thus obtained varies considerably from one cow to the next. The aim of the present study was to gain a better understanding of the follicular conditions associated with low oocyte developmental competence. Granulosa cells from 94 Holstein cows in a commercial embryo production facility were collected following ovarian stimulation and coasting...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28418847/quercetin-delays-postovulatory-aging-of-mouse-oocytes-by-regulating-sirt-expression-and-mpf-activity
#17
HaiYang Wang, Yu-Jin Jo, Jeong Su Oh, Nam-Hyung Kim
If no fertilization occurs at an appropriate time after ovulation, oocyte quality deteriorates rapidly as a process called postovulatory aging. Because the postovulatory aging of oocytes has detrimental effects on embryo development and offspring, many efforts have been made to prevent oocyte aging. Here we showed that quercetin prevented the decline in oocyte quality during postovulatory aging of oocytes. Quercetin treatment reduced aging-induced morphological changes and reactive oxygen species accumulation...
March 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28417349/a-higher-prevalence-of-endometriosis-among-asian-women-does-not-contribute-to-poorer-ivf-outcomes
#18
Ayae Yamamoto, Erica B Johnstone, Michael S Bloom, Heather G Huddleston, Victor Y Fujimoto
PURPOSE: The purpose of the study was to determine whether diagnosis of endometriosis or endometriosis with endometrioma influences in vitro fertilization (IVF) outcomes in an ethnically diverse population. METHODS: Women undergoing a first IVF cycle (n = 717) between January 1, 2008 and December 31, 2009, at a university-affiliated infertility clinic, were retrospectively assessed for an endometriosis diagnosis. Differences in prevalence of endometriosis by ethnicity were determined, as well as differences in IVF success by ethnicity, with a focus on country of origin for Asian women...
April 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28417052/relationship-between-meiotic-spindles-visualization-and-intracytoplasmic-sperm-injection-outcomes-in-human-oocytes
#19
Elham Asa, Reza Tabatabaee, Ahmad Farrokhi, Reza Nejatbakhsh
In assisted reproductive techniques, the operator attempts to select morphologically best embryos to predict embryo viability. Development of polarized light microscope, which evaluates the oocytes' spindles according to birefringence of living cells, had been helpful in oocyte selection. The aim of this study is evaluating the relationship between meiotic spindles visualization and intracytoplasmic sperm injection (ICSI) outcomes in human oocytes. In this study, 264 oocytes from 24 patients with an average age of 30...
March 2017: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/28407862/lipid-profiles-of-follicular-fluid-from-cows-submitted-to-ovarian-superstimulation
#20
P H Santos, P K Fontes, F F Franchi, M F G Nogueira, K R A Belaz, A Tata, M N Eberlin, M J Sudano, C M Barros, A C S Castilho
Ovarian superstimulation with exogenous gonadotropins has been extensively used to produce in vivo-derived embryos for embryo transfer in cattle. This process modifies the antral follicle microenvironment and affects oocyte and embryo quality as well the differentiation of granulosa cells. Lipids play significant roles in the cell, such as energy storage, cell structure, and fine-tuning of the physical properties and functions of biological membranes. The phospholipid (PL) contents as well as the effects of superstimulatory treatments on the PL profile of follicular fluid from cows, however, remain unknown...
May 2017: Theriogenology
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