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https://www.readbyqxmd.com/read/28229212/the-effect-of-a-cyclic-uniaxial-strain-on-urinary-bladder-cells
#1
Dorien Tiemessen, Paul de Jonge, Willeke Daamen, Wout Feitz, Paul Geutjes, Egbert Oosterwijk
PURPOSE: Pre-conditioning of a cell seeded construct may improve the functional outcome of a tissue engineered construct for augmentation cystoplasty. The precise effects of mechanical stimulation on urinary bladder cells in vitro are not clear. In this study we investigate the effect of a cyclic uniaxial strain culture on urinary bladder cells which were seeded on a type I collagen scaffold. METHODS: Isolated porcine smooth muscle cells or urothelial cells were seeded on a type I collagen scaffolds and cultured under static and dynamic conditions...
February 23, 2017: World Journal of Urology
https://www.readbyqxmd.com/read/28228615/levels-of-cyclic-amp-and-cyclic-gmp-in-porcine-oocyte-cumulus-complexes-and-cumulus-free-oocytes-derived-from-small-and-middle-follicles-during-the-first-24-hour-period-of-in-vitro-maturation
#2
Yuichi Okudaira, Takuya Wakai, Hiroaki Funahashi
The objective of this study was to compare the cAMP and cGMP levels in cumulus-oocyte complexes (COCs) derived from the middle follicles (MFs, 3-6 mm in diameter) and small follicles (SFs, 1-3 mm in diameter) of pre-pubertal gilts during the first 24-h period of maturation in vitro (IVM). Both cAMP and cGMP levels in MF- and SF-derived oocytes did not change during this period. Although the cAMP levels increased in the COCs at 10 and 20 h after the start of IVM, the levels of cAMP were significantly higher in MF-derived COCs than in SF-derived COCs at 20 h after the start of IVM...
February 23, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28228529/recapitulation-of-physiological-spatiotemporal-signals-promotes-in-vitro-formation-of-phenotypically-stable-human-articular-cartilage
#3
Johnathan J Ng, Yiyong Wei, Bin Zhou, Jonathan Bernhard, Samuel Robinson, Aonnicha Burapachaisri, X Edward Guo, Gordana Vunjak-Novakovic
Standard isotropic culture fails to recapitulate the spatiotemporal gradients present during native development. Cartilage grown from human mesenchymal stem cells (hMSCs) is poorly organized and unstable in vivo. We report that human cartilage with physiologic organization and in vivo stability can be grown in vitro from self-assembling hMSCs by implementing spatiotemporal regulation during induction. Self-assembling hMSCs formed cartilage discs in Transwell inserts following isotropic chondrogenic induction with transforming growth factor β to set up a dual-compartment culture...
February 22, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28224370/a-dual-mode-bioreactor-system-for-tissue-engineered-vascular-models
#4
N Bono, S Meghezi, M Soncini, M Piola, D Mantovani, Gianfranco Beniamino Fiore
In the past decades, vascular tissue engineering has made great strides towards bringing engineered vascular tissues to the clinics and, in parallel, obtaining in-lab tools for basic research. Herein, we propose the design of a novel dual-mode bioreactor, useful for the fabrication (construct mode) and in vitro stimulation (culture mode) of collagen-based tubular constructs. Collagen-based gels laden with smooth muscle cells (SMCs) were molded directly within the bioreactor culture chamber. Based on a systematic characterization of the bioreactor culture mode, constructs were subjected to 10% cyclic strain at 0...
February 21, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28223815/antitumor-effects-of-oncolytic-herpes-simplex-virus-type-2-against-colorectal-cancer-in-vitro-and-in-vivo
#5
Lei Yin, Chunhong Zhao, Jixia Han, Zengjun Li, Yanan Zhen, Ruixue Xiao, Zhongfa Xu, Yanlai Sun
BACKGROUND: The incidence of colorectal cancer (CRC) is on the rise. Furthermore, late-stage diagnoses and limited efficacious treatment options make CRC a complex clinical challenge. Therefore, a new therapeutic regimen with a completely novel therapeutic mechanism is necessary for CRC. In the present study, the therapeutic efficacy of oncolytic herpes simplex virus type 2 (oHSV2) in CRC was assessed in vitro and in vivo. oHSV2 is an oncolytic agent derived from herpes simplex virus type 2 that encodes granulocyte-macrophage colony-stimulating factor...
2017: Therapeutics and Clinical Risk Management
https://www.readbyqxmd.com/read/28223684/dendritic-cells-derived-exosomes-migration-to-spleen-and-induction-of-inflammation-are-regulated-by-ccr7
#6
Gao Wei, Yuan Jie, Liu Haibo, Wu Chaoneng, Huang Dong, Zhu Jianbing, Guo Junjie, Ma Leilei, Shi Hongtao, Zou Yunzeng, Ge Junbo
Mature dendritic cells (DCs) home to secondary lymphoid organs through CC chemokine receptor 7 (CCR7). Exosomes derived from DCs (DC-exos) are reported to migrate to spleen and induce inflammation in vivo. In this study, we demonstrated that mature bone marrow DC-exos can activate immature DC and T cells in vitro. Then we intravenously injected DC-exos into C57BL/6 mice, observing that mature DC-exos accumulated more in spleen than immature DC-exos. These DC-exos in spleen could be uptaken by splenetic DCs and T cells and induce an inflammatory response...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220575/generation-of-storable-retinal-organoids-and-retinal-pigmented-epithelium-from-adherent-human-ips-cells-in-xeno-free-and-feeder-free-conditions
#7
Sacha Reichman, Amélie Slembrouck, Giuliana Gagliardi, Antoine Chaffiol, Angélique Terray, Céline Nanteau, Anais Potey, Morgane Belle, Oriane Rabesandratana, Jens Duebel, Gael Orieux, Emeline F Nandrot, José-Alain Sahel, Olivier Goureau
Human induced pluripotent stem cells (hiPSCs) are potentially useful in regenerative therapies for retinal disease. For medical applications, therapeutic retinal cells, such as retinal pigmented epithelial (RPE) cells or photoreceptor precursors, must be generated under completely defined conditions. To this purpose, we have developed a two-step xeno-free/feeder-free (XF/FF) culture system to efficiently differentiate hiPSCs into retinal cells. This simple method, relies only on adherent hiPSCs cultured in chemically defined media, bypassing embryoid body formation...
February 20, 2017: Stem Cells
https://www.readbyqxmd.com/read/28219951/generation-of-thalamic-neurons-from-mouse-embryonic-stem-cells
#8
Atsushi Shiraishi, Keiko Muguruma, Yoshiki Sasai
Thalamus is a diencephalic structure that plays crucial roles in relaying and modulating sensory and motor information to the neocortex. The thalamus develops in the dorsal part of the neural tube at the level of the caudal forebrain. However, the molecular mechanisms that are essential for thalamic differentiation are still unknown. Here we have succeeded in the generation of thalamic neurons from mouse ES cells (mESCs) by modifying the default method that induces the most-anterior neural type in self-organizing culture...
February 20, 2017: Development
https://www.readbyqxmd.com/read/28217816/candida-albicans-survival-growth-and-biofilm-formation-are-differently-affected-by-mouthwashes-an-in-vitro-study
#9
Simona Paulone, Giulia Malavasi, Andrea Ardizzoni, Carlotta Francesca Orsi, Samuele Peppoloni, Rachele Giovanna Neglia, Elisabetta Blasi
Candida albicans is the most common cause of oral mycoses. The aim of the present study was to investigate in vitro the susceptibility of C. albicans to mouthwashes, in terms of growth, survival and biofilm formation. Candida albicans, laboratory strain SC5314, and 7 commercial mouthwashes were employed: 3 with 0.2% chlorhexidine digluconate; 1 with 0.06% chlorhexidine digluconate and 250 ppm F- sodium fluoride; 3 with fluorine-containing molecules. None of the mouthwashes contained ethanol in their formulations...
January 2017: New Microbiologica
https://www.readbyqxmd.com/read/28216492/-improvement-in-isolation-and-culture-method-for-oligodendrocyte-precursor-cells-and-establishment-of-the-in-vitro-model-for-lineage-restricted-oligodendrocyte-differentiation
#10
Xin Wang, Junmei Xu, Yaping Wang, Lin Yang
To establish the improved methods for oligodendrocyte precursor cells (OPCs) isolation, culture and subculture and to investigate the characteristics of OPCs differentiation and proliferation in vitro.
 Methods: OPCs isolated from neonatal SD rat spinal cords were cultured in vitro. Cell proliferation was induced by the culture solution with basic fibroblast growth factor and platelet-derived growth factor, and the proliferation of OPCs was determined by MTT assay. OPCs differentiation to mature oligodendrocytes was induced by triiodothyronine and ciliary neurotrophic factor...
January 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28215928/human-platelet-lysate-is-a-successful-alternative-serum-supplement-for-propagation-of-monocyte-derived-dendritic-cells
#11
Urban Švajger
BACKGROUND AIMS: Clinical protocols for dendritic cell (DC) generation from monocytes require the use of animal serum-free supplements. Serum-free media can also require up to 1% of serum supplementation. In addition, recommendations based on the 3Rs (Refinement, Reduction, Replacement) principle also recommend the use of non-animal sera in in vitro studies. The aim of this study was to explore the potential use of platelet lysate (PL) for generation of optimally differentiated DCs from monocytes...
February 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28215680/production-of-sperm-from-porcine-fetal-testicular-tissue-after-cryopreservation-and-grafting-into-nude-mice
#12
Hiroyuki Kaneko, Kazuhiro Kikuchi, Nguyen Thi Men, Michiko Nakai, Junko Noguchi, Naomi Kashiwazaki, Junya Ito
A major goal of testicular xenografting is to salvage germ cells from immature animals that cannot be used for reproduction and generate their offspring. In this study, we investigated whether porcine fetal testicular tissue would acquire the ability to produce sperm with full developmental competence after they had been cryopreserved and grafted into nude mice. Testicular fragments from fetuses at 35, 55 and 90 days postartificial insemination (dpi) were vitrified and stored in liquid nitrogen. Immediately after warming, testicular fragments at each fetal stage were transplanted under the back skin of castrated nude mice (Crlj:CD1-Foxn1(nu)) (35-, 55- and 90-dpi groups, respectively) (day 0 = grafting)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215678/the-impact-of-ovarian-stimulation-protocol-on-oocyte-quality-subsequent-in%C3%A2-vitro-embryo-development-and-pregnancy-after-transfer-to-recipients-in-eld-s-deer-rucervus-eldii-thamin
#13
Ampika Thongphakdee, Debra K Berg, Theerawat Tharasanit, Nikorn Thongtip, Wanlaya Tipkantha, Chainarong Punkong, Daraka Tongthainan, Sakhon Noimoon, Umaporn Maikeaw, Nudthakamol Kajornklin, Boripat Siriaroonrat, Pierre Comizzoli, Sumate Kamolnorranath
Propagating genetically valuable individuals through oocyte collection, in vitro fertilization (IVF) and embryo transfer is critical to maintain sustainable populations of the endangered Eld's deer. The objectives of this study were to assess the impact of exogenous FSH injections on (1) the number and in vitro competence of oocytes collected and (2) the developmental potential of resulting IVF embryos after transfer into recipients during the breeding season (February-April). In a pilot experiment, estrus synchronization was conducted in three surplus females (using intravaginal progesterone-releasing devices, CIDRG for 14 days and injections of buserelin (a GnRH agonist)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215677/pyridoxine-supplementation-during-oocyte-maturation-improves-the-development-and-quality-of-bovine-preimplantation-embryos
#14
Mansour Aboelenain, Ahmed Zaky Balboula, Manabu Kawahara, Abd El-Monem Montaser, Samy Moawad Zaabel, Sung-Woo Kim, Masashi Nagano, Masashi Takahashi
Recently, inhibition of cathepsin B (CTSB) activity during in vitro maturation (IVM) and culture (IVC) improved the developmental competence and quality of bovine oocytes and embryos. E-64 is a widely used inhibitor to inhibit CTSB activity, however, E-64 inhibits not only CTSB activity but also the activities of other proteases including cathepsin L (CTSL), papain, calpain, and trypsin. Pyridoxine, the catalytically active form of vitamin B6, plays a crucial role in several cellular processes and has the ability to inhibit CTSB activity...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215674/effect-of-vitrification-on-meiotic-maturation-mitochondrial-distribution-and-glutathione-synthesis-in-immature-silver-fox-cumulus-oocyte-complexes
#15
Xinyan Cao, Jingchun Li, Hailong Xue, Shiyong Wang, Weigang Zhao, Zhanyu Du, Yifeng Yang, Zhigang Yue
The present study was designed to investigate the effects of vitrifying oocytes obtained from silver foxes on nuclear maturation, mitochondrial distribution and glutathione (GSH) synthesis after in vitro culture for 72 h. Immature oocytes were randomly divided into three groups: (1) fresh GV (germinal vesicle) oocytes (Control group), (2) exposure to the equilibration and vitrification solution but without being plunged into liquid nitrogen (exposed group), and (3) vitrification by the cryoloop method (vitrified-warmed group)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215673/supplement-of-cilostamide-in-growth-medium-improves-oocyte-maturation-and-developmental-competence-of-embryos-derived-from-small-antral-follicles-in-pigs
#16
Hanna Lee, Fazle Elahi, Joohyeong Lee, Seung Tae Lee, Sang-Hwan Hyun, Eunsong Lee
This study was conducted to evaluate the effects of cyclic AMP (cAMP) modulator cilostamide (CIL) and forskolin (FSK) treatment during in vitro growth (IVG) on growth, maturation, and embryonic development of cumulus-oocyte complexes (COCs) derived from small antral follicles < 3 mm in diameter (SAFCOCs). SAFCOCs were untreated (control) or treated with 20 μM CIL and/or 50 μM FSK for 2 days for IVG. Next, IVG oocytes were cultured for maturation and then induced for parthenogenesis (PA) or used as recipient ooplasts for somatic cell nuclear transfer (SCNT)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215626/dendritic-cells-that-highly-express-socs1-induce-t-cell-hypo-responsiveness-and-prolong-islet-allograft-survival
#17
Xinjun Lu, Maogen Chen, Zhicheng Xue, Xuzhi Zhang, Jiejie Xu, Linwei Wu, Ronghai Deng, Yi Ma
The capability of dendritic cells (DCs) to induce an immune response or immune tolerance is dependent on their status. Suppressor of cytokine signaling 1 (SOCS1) is a pivotal regulator that participates in negative feedback of the JAK-STAT pathway, which plays a key role in the differentiation, activation, and maturation of DCs. DCs that highly express SOCS1 may modulate DCs, and induce immune anergy or immune tolerance. In this study, we transduced DCs with the recombinant adenovirus Ad5F35 to highly express SOCS1...
February 2, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28213717/the-minor-collagens-in-articular-cartilage
#18
REVIEW
Yunyun Luo, Dovile Sinkeviciute, Yi He, Morten Karsdal, Yves Henrotin, Ali Mobasheri, Patrik Önnerfjord, Anne Bay-Jensen
Articular cartilage is a connective tissue consisting of a specialized extracellular matrix (ECM) that dominates the bulk of its wet and dry weight. Type II collagen and aggrecan are the main ECM proteins in cartilage. However, little attention has been paid to less abundant molecular components, especially minor collagens, including type IV, VI, IX, X, XI, XII, XIII, and XIV, etc. Although accounting for only a small fraction of the mature matrix, these minor collagens not only play essential structural roles in the mechanical properties, organization, and shape of articular cartilage, but also fulfil specific biological functions...
February 17, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28212670/characterization-of-schistosoma-japonicum-cp1412-protein-as-a-novel-member-of-the-ribonuclease-t2-molecule-family-with-immune-regulatory-function
#19
Xue-Dan Ke, Shuang Shen, Li-Jun Song, Chuan-Xin Yu, Mihoko Kikuchi, Kenji Hirayama, Hong Gao, Jie Wang, Xuren Yin, Yuan Yao, Qian Liu, Wei Zhou
BACKGROUND: Schistosome infection typically induces a polarized Th2 type host immune response. As egg antigen molecules play key roles in this immunoregulatory process, clarifying their functions in schistosomiasis would facilitate the development of vaccine and immunotherapeutic methods. Schistosoma japonicum (Sj) CP1412 (GenBank: AY57074.1) has been identified as a new member of the RNase T2 family with immune regulatory functions. METHODS: The expression plasmid Sj CP1412-pET28a was constructed and transformed into bacteria for production of recombinant Sj CP1412 protein (rSj CP1412) via IPTG induction...
February 17, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28212450/expression-and-activity-of-multidrug-resistance-proteins-in-mature-endothelial-cells-and-their-precursors-a-challenging-correlation
#20
Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Karolina Wojtowicz, Roksana Jura, Maria Paprocka, Elżbieta Wojdat, Urszula Kozłowska, Aleksandra Klimczak, Catherine Grillon, Claudine Kieda, Danuta Duś
Active cellular transporters of harmful agents-multidrug resistance (mdr) proteins-are present in tumor, stem and endothelial cells, among others. While mdr proteins are broadly studied in tumor cells, their role in non-tumor cells and the significance of their action not connected with removal of harmful xenobiotics is less extensively documented. Proper assessment of mdr proteins expression is difficult. Mdr mRNA presence is most often evaluated but that does not necessarily correlate with the protein level...
2017: PloS One
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