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in vitro maturation

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https://www.readbyqxmd.com/read/28934466/cohesin-acetyltransferase-esco2-regulates-sac-and-kinetochore-functions-via-maintaining-h4k16-acetylation-during-mouse-oocyte-meiosis
#1
Yajuan Lu, Xiaoxin Dai, Mianqun Zhang, Yilong Miao, Changyin Zhou, Zhaokang Cui, Bo Xiong
Sister chromatid cohesion, mediated by cohesin complex and established by the acetyltransferases Esco1 and Esco2, is essential for faithful chromosome segregation. Mutations in Esco2 cause Roberts syndrome, a developmental disease characterized by severe prenatal retardation as well as limb and facial abnormalities. However, its exact roles during oocyte meiosis have not clearly defined. Here, we report that Esco2 localizes to the chromosomes during oocyte meiotic maturation. Depletion of Esco2 by morpholino microinjection leads to the precocious polar body extrusion, the escape of metaphase I arrest induced by nocodazole treatment and the loss of BubR1 from kinetochores, indicative of inactivated SAC...
September 19, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28932644/romidepsin-alone-or-in-combination-with-anti-cd20-chimeric-antigen-receptor-expanded-natural-killer-cells-targeting-burkitt-lymphoma-in-vitro-and-in-immunodeficient-mice
#2
Yaya Chu, Ashlin Yahr, Brian Huang, Janet Ayello, Matthew Barth, Mitchell S Cairo
Facilitating the development of alternative targeted therapeutic strategies is urgently required to improve outcome or circumvent chemotherapy resistance in children, adolescents, and adults with recurrent/refractory de novo mature B-cell (CD20) non-Hodgkin lymphoma, including Burkitt lymphoma (BL). Romidepsin, a histone deacetylase inhibitor (HDACi), has been used to treat cutaneous T-cell lymphoma. We have demonstrated the significant anti-tumor effect of anti-CD20 chimeric antigen receptor (CAR) modified expanded peripheral blood natural killer (exPBNK) against rituximab-sensitive and -resistant BL...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28931865/use-of-medroxyprogesterone-acetate-in-women-with-ovarian-endometriosis-undergoing-controlled-ovarian-hyperstimulation-for-in-vitro-fertilization
#3
Haiyan Guo, Yun Wang, Qiuju Chen, Weiran Chai, Lihua Sun, Ai Ai, Yonglun Fu, Qifeng Lyu, Yanping Kuang
This study investigated the use of medroxyprogesterone acetate (MPA) or a short protocol for controlled ovarian hyperstimulation (COH) in patients with advanced endometriosis who have normal ovarian function, and to compare cycle characteristics and pregnancy outcomes after frozen-thawed embryo transfer (FET). This was a retrospective case-control study of 244 patients with advanced endometriosis undering COH. The patients were allocated to three groups: the surgery group with MPA COH (62 patients, 71 IVF/ICSI cycles, 78 FET cycles); the aspiration group with MPA COH (85 patients had ovarian "chocolate" cysts (>3 cm) aspirated, 90 IVF/ICSI cycles, 76 FET cycles); and the short protocol group (97 patients, 101 IVF/ICSI cycles, 51 FET cycles)...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931727/co-culture-of-allogenic-dbm-and-bmscs-in-the-knee-joint-cavity-of-rabbits-for-cartilage-tissue-engineering
#4
Bin Xu, Rui Wang, Hao Wang, Hong-Gang Xu
This study aims to assess co-culture of allogenic decalcified bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) in the knee joint cavity of rabbits for cartilage tissue engineering. Rabbits were assigned to an in-vitro group, an in-vivo group and a blank control group. At the 4(th), 8(th) and 12(th) week, samples from all groups were collected for hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) method. The morphological analysis software was used to calculate the average absorbance value (A value)...
September 20, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28931519/beta-cell-replacement-in-mice-using-human-type-1-diabetes-nuclear-transfer-embryonic-stem-cells
#5
Lina Sui, Nichole Danzl, Sean R Campbell, Ryan Viola, Damian Williams, Yuan Xing, Yong Wang, Neil Phillips, Greg Poffenberger, Bjarki Johannesson, Jose Oberholzer, Alvin C Powers, Rudolph L Leibel, Xiaojuan Chen, Megan Sykes, Dieter Egli
Beta cells derived from stem cells hold great promise for cell replacement therapy for diabetes. Here we examine the ability of nuclear transfer embryonic stem cells (NT-ES) derived from a type 1 diabetes patient to differentiate into beta cells, and provide a source of autologous islets for cell replacement. NT-ES cells differentiate in vitro with an average efficiency of 55% into C-peptide-positive cells, expressing markers of mature beta cells, including MAFA and NKX6.1. Upon transplantation in immunodeficient mice, grafted cells form vascularized islet-like structures containing MAFA/C-peptide-positive cells...
September 20, 2017: Diabetes
https://www.readbyqxmd.com/read/28930267/%C3%AE-selinene-rich-essential-oils-from-the-parts-of-callicarpa-macrophylla-and-their-antioxidant-and-pharmacological-activities
#6
Mahesh Chandra, Om Prakash, Ravendra Kumar, Rakesh Kumar Bachheti, Brij Bhushan, Mahesh Kumar, Anil Kumar Pant
Background:Callicarpa macrophylla (Varbenaceae) is a medicinal shrub and is traditionally used in India, China, and South Asia. Methods: The plant material was collected from lower Himalayan region of Uttarakhand in India. The essential oils from three different aerial parts were analyzed by GC-MS. Antioxidant activity, phenolic assay, and various pharmacological activities were determined by using existing methods which are generally practiced widely. Results: Over 51, 53, and 40 compounds were identified in C...
July 10, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28929981/the-presence-of-acylated-ghrelin-during-in-vitro-maturation-of-bovine-oocytes-induces-cumulus-cell-dna-damage-and-apoptosis-and-impairs-early-embryo-development
#7
Matias A Sirini, Juan Mateo Anchordoquy, Juan Patricio Anchordoquy, Ana M Pascua, Noelia Nikoloff, Ana Carranza, Alejandro E Relling, Cecilia C Furnus
The aim of this study was to investigate the effects of acylated ghrelin supplementation during in vitro maturation (IVM) of bovine oocytes. IVM medium was supplemented with 20, 40 or 60 pM acylated ghrelin concentrations. Cumulus expansion area and oocyte nuclear maturation were studied as maturation parameters. Cumulus-oocyte complexes (COC) were assessed with the comet, apoptosis and viability assays. The in vitro effects of acylated ghrelin on embryo developmental capacity and embryo quality were also evaluated...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#8
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929461/the-isolation-culture-and-propagation-of-murine-intestinal-enteroids-for-the-study-of-dietary-lipid-metabolism
#9
Diana Li, Hongli Dong, Alison B Kohan
Since the initial report in 2009 by Sato and Clevers, primary enteroids have been of major interest in the fields of stem cell biology and gastrointestinal (GI) tract biology. More recently, we and others have made major inroads into the physiological relevance of these enteroid models and have shown that enteroids derived from the stomach, intestine, or colon recapitulate major functions of these tissues, namely, gastric acid secretion, lipid absorption and lipoprotein secretion, and ion transport. Here, we detail the isolation of stem cells from the small intestine and the culture and propagation of those stem cells into mature three-dimensional enteroids...
September 20, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28928717/egg-quality-during-the-pubertal-transition-is-youth-all-it-s-cracked-up-to-be
#10
Francesca E Duncan
Although it is well accepted that egg quality decreases with advanced maternal age, we do not know how it is affected at the earliest ages during the pubertal transition-likely because this young population is not typically reproducing. However, in the setting of fertility preservation, more childhood cancer patients are surviving their diagnosis due to medical advances, forcing patients and their families to consider their future fertility at a very young age. Ex vivo in vitro maturation, in which cumulus oocyte complexes harvested from ovarian tissue are cultured to obtain mature gametes, is gaining traction as a fertility preservation method that is coupled to ovarian tissue cryopreservation...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28927438/activated-ovarian-endothelial-cells-promote-early-follicular-development-and-survival
#11
Alon Kedem, Anate Aelion-Brauer, Peipei Guo, Duancheng Wen, Bi-Sen Ding, Raphael Lis, Du Cheng, Vladislav M Sandler, Shahin Rafii, Zev Rosenwaks
BACKGROUND: New data suggests that endothelial cells (ECs) elaborate essential "angiocrine factors". The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. METHODS: Mouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) (n = 32), gel (n = 24) or within matrigel (n = 27) in serum free media for 14 days...
September 19, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28927010/the-role-of-oocyte-organelles-in-determining-developmental-competence
#12
REVIEW
Karen L Reader, Jo-Ann L Stanton, Jennifer L Juengel
The ability of an oocyte to undergo successful cytoplasmic and nuclear maturation, fertilization and embryo development is referred to as the oocyte's quality or developmental competence. Quality is dependent on the accumulation of organelles, metabolites and maternal RNAs during the growth and maturation of the oocyte. Various models of good and poor oocyte quality have been used to understand the essential contributors to developmental success. This review covers the current knowledge of how oocyte organelle quantity, distribution and morphology differ between good and poor quality oocytes...
September 18, 2017: Biology
https://www.readbyqxmd.com/read/28926981/antibiofilm-activity-and-mechanism-of-action-of-the-disinfectant-chloramine-t-on-candida-spp-and-its-toxicity-against-human-cells
#13
Gabriela Lacet Silva Ferreira, Pedro Luiz Rosalen, Larissa Rangel Peixoto, Ana Luiza Alves de Lima Pérez, Fabíola Galbiatti de Carvalho Carlo, Lúcio Roberto Cançado Castellano, Jefferson Muniz de Lima, Irlan Almeida Freires, Edeltrudes de Oliveira Lima, Ricardo Dias de Castro
We evaluated the antifungal and anti-biofilm activity, mechanism of action and cytotoxicity of chloramine T trihydrate (CAT) against Candida spp. The Minimum Inhibitory and Fungicidal Concentrations (MIC/MFC) of CAT were determined. Changes in CAT-treated C. albicans growth kinetics and micromorphology were evaluated, as well as the mechanism of action, and its effects on biofilm. Cytotoxicity was assessed by the hemolysis method. The data were analyzed by inferential statistics (p ≤ 0.05). CAT showed antifungal activity against all strains, with MIC values ranging between 1...
September 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28923825/qualitative-differences-in-t-cell-activation-by-dendritic-cell-derived-extracellular-vesicle-subtypes
#14
Mercedes Tkach, Joanna Kowal, Andres E Zucchetti, Lotte Enserink, Mabel Jouve, Danielle Lankar, Michael Saitakis, Lorena Martin-Jaular, Clotilde Théry
Exosomes, nano-sized secreted extracellular vesicles (EVs), are actively studied for their diagnostic and therapeutic potential. In particular, exosomes secreted by dendritic cells (DCs) have been shown to carry MHC-peptide complexes allowing efficient activation of T lymphocytes, thus displaying potential as promoters of adaptive immune responses. DCs also secrete other types of EVs of different size, subcellular origin and protein composition, whose immune capacities have not been yet compared to those of exosomes...
September 18, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28923708/enhanced-in%C3%A2-vitro-maturation-of-canine-oocytes-by-oviduct-epithelial-cell-co-culture
#15
Jingu No, Minghui Zhao, Seunghoon Lee, Sun A Ock, Yoonseok Nam, Tai-Young Hur
Canine-assisted reproductive techniques have been successful for several years; however, the lack of an oocyte in vitro maturation system has limited their application. The aim of this study was to evaluate the effect of canine oviduct epithelial cells (cOECs) on canine oocyte maturation in vitro. Specifically, the method used for isolation of cOECs did not affect the expression of epithelial markers, E-cadherin and cytokeratin, on fresh, cultured and cryopreserved cells. Moreover, BrdU analysis showed that cOECs cultured in Medium 171 supplemented with mammary epithelial growth supplement were more proliferative than counterparts in advanced Dulbecco's modified Eagle medium or Medium 199...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923420/high-doses-of-lipid-core-nanocapsules-do-not-affect-bovine-embryonic-development-in-vitro
#16
Caroline Lucas, Mariana Remião, Franciele Bruinsmann, Isadora Lopes, Morgana Borges, Ana Laura Feijó, Andrea Cristina Basso, Adriana Pohlmann, Silvia Guterres, Vinicius Campos, Fabiana Seixas, Tiago Collares
The improvement of in vitro embryo production by culture media supplementation has been a potential tool to increase blastocyst quality and development. Recently, lipid-core nanocapsules (LNC), which were developed for biomedical applications as a drug-delivery system, have demonstrated beneficial effects on in vitro embryo production studies. LNCs have a core composed of sorbitan monostearate dispersed in capric/caprylic triglyceride. Based on that, we firstly investigated if LNCs supplemented during in vitro oocyte maturation had affinity to the mineral oil placed over the top of the IVM media...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28923241/opposing-roles-of-epidermal-integrins-%C3%AE-3%C3%AE-1-and-%C3%AE-9%C3%AE-1-in-regulation-of-mtld-bmp-1-mediated-laminin-%C3%AE-2-processing-during-wound-healing
#17
Whitney M Longmate, Scott P Lyons, Lori DeFreest, Livingston Van De Water, C Michael DiPersio
Proteolytic processing of the laminin-γ2 chain is a hallmark of basement membrane maturation in the skin. Integrin α3β1, a major receptor for epidermal adhesion to laminin-332, is critical for proper basement membrane organization during skin development and wound healing. Previously, we identified a role for α3β1 in promoting the processing of laminin-γ2 in cultured keratinocytes in vitro and in wound epidermis in vivo. In the current study we identify the Bmp1 gene, which encodes variants of the mammalian tolloid/bone morphogenetic protein-1 metalloproteases, as a critical regulator of α3β1-dependent laminin-γ2 processing, thereby expanding the role of this integrin in controlling the secretion by the epidermis of factors that modulate the tissue microenvironment...
September 15, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28923001/a-molecular-view-of-the-normal-human-thyroid-structure-and-function-reconstructed-from-its-reference-transcriptome-map
#18
Lorenza Vitale, Allison Piovesan, Francesca Antonaros, Pierluigi Strippoli, Maria Chiara Pelleri, Maria Caracausi
BACKGROUND: The thyroid is the earliest endocrine structure to appear during human development, and thyroid hormones are necessary for proper organism development, in particular for the nervous system and heart, normal growth and skeletal maturation. To date a quantitative, validated transcriptional atlas of the whole normal human thyroid does not exist and the availability of a detailed expression map might be an excellent occasion to investigate the many features of the thyroid transcriptome...
September 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28921686/the-effects-of-dynamical-synapses-on-firing-rate-activity-a-spiking-neural-network-model
#19
Radwa Khalil, Marie Z Moftah, Ahmed A Moustafa
Accumulating evidence relates the fine-tuning of synaptic maturation and regulation of neural network activity to several key factors, including GABAA signaling and a lateral spread length between neighboring neurons (i.e. local connectivity). Furthermore, a number of studies consider Short-Term synaptic Plasticity (STP) as an essential element in the instant modification of synaptic efficacy in the neuronal network and in modulating responses to sustained ranges of external Poisson Input Frequency (IF). Nevertheless, evaluating the firing activity in response to the dynamical interaction between STP (triggered by ranges of IF), and these key parameters in vitro remains elusive...
September 18, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28921552/immunomodulatory-effect-of-cryopreserved-platelets-altered-bdca3-dendritic-cell-maturation-and-activation-in-vitro
#20
Katrina K Ki, Lacey Johnson, Helen M Faddy, Robert L Flower, Denese C Marks, Melinda M Dean
BACKGROUND: Cryopreservation of platelets (PLTs) is useful in remote areas to overcome logistic problems associated with supply and can extend the shelf life to 2 years. During cryopreservation, properties of PLTs are modified. Whether changes in the cryopreserved PLT (CPP) product are associated with modulation of recipients' immune function is unknown. We aimed to characterize the immune profile of myeloid dendritic cells (mDCs) and the specialized blood DC antigen (BDCA)3(+) subset after exposure to CPPs...
September 16, 2017: Transfusion
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