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https://www.readbyqxmd.com/read/29156741/screening-epitopes-on-systemic-lupus-erythematosus-autoantigens-with-a-peptide-array
#1
Lin Wang, Chenjun Hao, Yongqiu Deng, Yanbo Liu, Shiliang Hu, Yangang Peng, Manna He, Jinhu Fu, Ming Liu, Jia Chen, Xiaoming Chen
Systemic lupus erythematosus (SLE) is a common autoimmune disease. Many autoantibodies are closely associated with SLE. However, the specific epitopes recognized and bound by these autoantibodies are still unclear. This study screened the binding epitopes of SLE-related autoantibodies using a high-throughput screening method. Epitope prediction on 12 SLE-related autoantigens was performed using the Immune Epitope Database and Analysis Resource (IEDB) software. The predicted epitopes were synthesized into peptides and developed into a peptide array...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156380/structure-based-design-of-herg-neutral-antihypertensive-oxazalone-and-imidazolone-derivatives
#2
Busecan Aksoydan, Isik Kantarcioglu, Ismail Erol, Ramin Ekhteiari Salmas, Serdar Durdagi
Angiotensin II receptor type 1 (AT1) antagonists are the most recent drug class against hypertension. Recently first crystal structure of AT1 receptor is deposited to the protein data bank (PDB ID: 4YAY). In this work, several molecular screening methods such as molecular docking and de novo design studies were performed and it is found that oxazolone and imidazolone derivatives reveal similar/better interaction energy profiles compared to the FDA approved sartan molecules at the binding site of the AT1 receptor...
October 18, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29156121/the-toxmatrix-chemo-genomic-profiling-identifies-interactions-that-reveal-mechanisms-of-toxicity
#3
Zhi-Bin Tong, Ruili Huang, Yuhong Wang, Carleen Klumpp-Thomas, John Braisted, Zina Itkin, Paul Shinn, Menghang Xia, Anton Simeonov, David Gerhold
A chemical genomics 'Toxmatrix' method was developed to elucidate mechanisms of cytotoxicity using neuronal models. Quantitative high-throughput screening (qHTS) was applied to systematically screen each toxicant against a panel of 70 modulators, drugs or chemicals that act on a known target, to identify interactions that either protect or sensitize cells to each toxicant. Thirty-two toxicants were tested at 10 concentrations for cytotoxicity to SH-SY5Y human neuroblastoma cells, with results fitted to the Hill equation to determine an IC50 for each toxicant...
November 20, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29156009/discovering-cancer-vulnerabilities-using-high-throughput-micro-rna-screening
#4
Iva Nikolic, Benjamin Elsworth, Eoin Dodson, Sunny Z Wu, Cathryn M Gould, Pieter Mestdagh, Glenn M Marshall, Lisa G Horvath, Kaylene J Simpson, Alexander Swarbrick
Micro-RNAs (miRNAs) are potent regulators of gene expression and cellular phenotype. Each miRNA has the potential to target hundreds of transcripts within the cell thus controlling fundamental cellular processes such as survival and proliferation. Here, we exploit this important feature of miRNA networks to discover vulnerabilities in cancer phenotype, and map miRNA-target relationships across different cancer types. More specifically, we report the results of a functional genomics screen of 1280 miRNA mimics and inhibitors in eight cancer cell lines, and its presentation in a sophisticated interactive data portal...
November 16, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29155762/high-throughput-analysis-of-locomotor-behavior-in-the-drosophila-island-assay
#5
Ilse Eidhof, Michaela Fenckova, Dei M Elurbe, Bart van de Warrenburg, Anna Castells Nobau, Annette Schenck
Advances in next-generation sequencing technologies contribute to the identification of (candidate) disease genes for movement disorders and other neurological diseases at an increasing speed. However, little is known about the molecular mechanisms that underlie these disorders. The genetic, molecular, and behavioral toolbox of Drosophila melanogaster makes this model organism particularly useful to characterize new disease genes and mechanisms in a high-throughput manner. Nevertheless, high-throughput screens require efficient and reliable assays that, ideally, are cost-effective and allow for the automatized quantification of traits relevant to these disorders...
November 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155731/the-nematode-caenorhabditis-elegans-a-versatile-in-vivo-model-to-study-host-microbe-interactions
#6
Luca Issi, Meredith Rioux, Reeta Rao
We demonstrate a method using Caenorhabditis elegans as a model host to study microbial interaction. Microbes are introduced via the diet making the intestine the primary location for disease. The nematode intestine structurally and functionally mimics mammalian intestines and is transparent making it amenable to microscopic study of colonization. Here we show that pathogens can cause disease and death. We are able to identify microbial mutants that show altered virulence. Its conserved innate response to biotic stresses makes C...
October 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29154949/a-novel-microscopy-based-assay-identifies-extended-synaptotagmin-1-esyt1-as-a-positive-regulator-of-anoctamin-1-traffic
#7
Joana R Lérias, Madalena C Pinto, Hugo M Botelho, Nikhil T Awatade, Margarida C Quaresma, Iris A L Silva, Podchanart Wanitchakool, Rainer Schreiber, Rainer Pepperkok, Karl Kunzelmann, Margarida D Amaral
An attractive possibility to treat Cystic Fibrosis (CF), a severe condition caused by dysfunctional CFTR, an epithelial anion channel, is through the activation of alternative (non-CFTR) anion channels. Anoctamin 1 (ANO1) was demonstrated to be a Ca(2+)-activated chloride channel (CaCC) and thus of high potential to replace CFTR. Despite that ANO1 is expressed in human lung CF tissue, it is present at the cell surface at very low levels. In addition, little is known about regulation of ANO1 traffic, namely which factors promote its plasma membrane (PM) localization...
November 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29154792/robust-real-time-cell-analysis-method-for-determining-viral-infectious-titers-during-development-of-a-viral-vaccine-production-process
#8
Cédric Charretier, Aure Saulnier, Loïc Benair, Corinne Armanet, Isabelle Bassard, Sandra Daulon, Bertrand Bernigaud, Emanuel Rodrigues de Sousa, Clémence Gonthier, Edouard Zorn, Emmanuelle Vetter, Claire Saintpierre, Patrice Riou, David Gaillac
The classical cell-culture methods, such as cell culture infectious dose 50% (CCID50) assays, are time-consuming, end-point assays currently used during the development of a viral vaccine production process to measure viral infectious titers. However, they are not suitable for handling the large number of tests required for high-throughput and large-scale screening analyses. Impedance-based bio-sensing techniques used in real-time cell analysis (RTCA) to assess cell layer biological status in vitro, provide real-time data...
November 14, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/29153953/inhibition-of-curcumin-on-influenza-a-virus-infection-and-influenzal-pneumonia-via-oxidative-stress-tlr2-4-p38-jnk-mapk-and-nf-%C3%AE%C2%BAb-pathways
#9
Jianping Dai, Liming Gu, Yun Su, Qianwen Wang, Ying Zhao, Xiaoxua Chen, Huixiong Deng, Weizhong Li, Gefei Wang, Kangsheng Li
Oxidative stress, Nrf2-HO-1 and TLR-MAPK/NF-κB signaling pathways have been proved to be involved in influenza A virus (IAV) replication and influenzal pneumonia. In the previous studies, we have performed several high-throughput drug screenings based on the TLR pathways. In the present study, through plaque inhibition test, luciferase reporter assay, TCID50, qRT-PCR, western blotting, ELISA and siRNA assays, we investigated the effect and mechanism of action of curcumin against IAV infection in vitro and in vivo...
November 15, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29153579/liquid-chromatography-coupled-to-quadrupole-orbitrap-high-resolution-mass-spectrometry-based-method-for-target-analysis-and-suspect-screening-of-non-ionic-surfactants-in-textiles
#10
Xin Luo, Li Zhang, Zengyuan Niu, Xiwen Ye, Zhixu Tang, Shuwei Xia
In this study, we describe a high-throughput and sensitive method for textiles analysis, using liquid chromatography coupled to quadrupole-Orbitrap high resolution mass spectrometry (LC-Q-Orbitrap HRMS), for the simultaneously quantitative analysis of 40 target alkylphenol polyethoxylates (APEO) oligomers with reference standards and screening of 160 alcohol polyethoxylates (AEO) oligomers without standards in textiles. The APEOs contain nonylphenol ethoxylates (NPEOs) and octylphenol ethoxylates (OPEOs) with an EO number of ethylene oxide of 1-20, while AEOs focus on C11EOs-C18EOs with an EO number of 1-20...
November 7, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29153031/human-co-culture-model-of-neurons-and-astrocytes-to-test-acute-cytotoxicity-of-neurotoxic-compounds
#11
Uliana De Simone, Francesca Caloni, Laura Gribaldo, Teresa Coccini
Alternative methods and their use in planning and conducting toxicology experiments have become essential for modern toxicologists, thus reducing or replacing living animals. Although in vitro human co-culture models allow the establishment of biologically relevant cell-cell interactions that recapitulate the tissue microenvironment and better mimic its physiology, the number of publications is limited specifically addressing this scientific area and utilizing this test method which could provide an additional valuable model in toxicological studies...
January 1, 2017: International Journal of Toxicology
https://www.readbyqxmd.com/read/29152631/mask-free-fabrication-of-a-versatile-microwell-chip-for-multidimensional-cellular-analysis-and-drug-screening
#12
Wenguang Yang, Haibo Yu, Gongxin Li, Fanan Wei, Yuechao Wang, Lianqing Liu
Cells are frequently studied because they are basic structural, functional, and biological units of living organisms. Extracting features from cellular behaviors can facilitate decision making in medical diagnoses and represents an important aspect in the development of biomedical engineering. Previous studies have just focused on either the individual cell or cell clusters separately, which leads to a great lack of information. Microwell technologies could address the challenges of in vitro cellular studies, from individual cell studies to 3D functional assays, by providing more information from smaller sample volumes and enabling the incorporation of low-cost high-throughput assays in the drug discovery process...
November 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/29151844/pym-a-new-affordable-image-based-method-using-a-raspberry-pi-to-phenotype-plant-leaf-area-in-a-wide-diversity-of-environments
#13
Benoît Valle, Thierry Simonneau, Romain Boulord, Francis Sourd, Thibault Frisson, Maxime Ryckewaert, Philippe Hamard, Nicolas Brichet, Myriam Dauzat, Angélique Christophe
Background: Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manual, often destructive operations, causing large errors. Plant imaging emerged as a viable alternative allowing non-invasive and automated data acquisition. Several procedures based on image analysis were developed to monitor leaf growth as a major phenotyping target. However, in most proposals, a time-consuming parameterization of the analysis pipeline is required to handle variable conditions between images, particularly in the field due to unstable light and interferences with soil surface or weeds...
2017: Plant Methods
https://www.readbyqxmd.com/read/29150881/genotoxicity-of-11-heavy-metals-detected-as-food-contaminants-in-two-human-cell-lines
#14
B Kopp, D Zalko, M Audebert
Heavy metals, such as arsenic (As), antimony (Sb), barium (Ba), cadmium (Cd), cobalt (Co), germanium (Ge), lead (Pb), nickel (Ni), tellurium (Te), and vanadium (V) are widely distributed in the environment and in the food chain. Human exposure to heavy metals through water and food has been reported by different international agencies. Although some of these heavy metals are essential elements for human growth and development, they may also be toxic at low concentrations due to indirect mechanisms. In this study, the genotoxic and cytotoxic properties of 15 different oxidation statuses of 11 different heavy metals were investigated using high-throughput screening (γH2AX assay) in two human cell lines (HepG2 and LS-174T) representative of target organs (liver and colon) for food contaminants...
November 18, 2017: Environmental and Molecular Mutagenesis
https://www.readbyqxmd.com/read/29150808/monitoring-enzymatic-degradation-of-emerging-contaminants-using-a-chip-based-robotic-nano-esi-ms-tool
#15
Lara F Stadlmair, Thomas Letzel, Johanna Graßmann
Up to now, knowledge of enzymes capable of degrading various contaminants of emerging concern (CEC) is limited, which is especially due to the lack of rapid screening methods. Thus, a miniaturized high-throughput setup using a chip-based robotic nanoelectrospray ionization system coupled to mass spectrometry has been developed to rapidly screen enzymatic reactions with environmentally relevant CECs. Three laccases, two tyrosinases, and two peroxidases were studied for their ability to transform ten pharmaceuticals and benzotriazole...
November 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29150709/simultaneous-detection-of-nadph-consumption-and-h2o2-production-using-the-ampliflu%C3%A2-red-assay-for-screening-of-p450-activities-and-uncoupling
#16
Lisa K Morlock, Dominique Böttcher, Uwe T Bornscheuer
Cytochrome P450s belong to a large and diverse group of heme-containing enzymes. These monooxygenases catalyze the incorporation of a single atom of molecular oxygen into their substrate. In contrast to most other enzymes, the activity of P450 enzymes is not only dependent on substrate and cofactor availability and reaction conditions, but also depends on the coupling efficiency of the catalytic cycle itself. Through the electron transfer from NAD(P)H to the heme-center of the P450, the enzyme becomes activated and binds oxygen...
November 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29150518/molecular-characterization-of-an-endozoicomonas-like-organism-causing-infection-in-king-scallop-pecten-maximus-l
#17
Irene Cano, Ronny van Aerle, Stuart Ross, David W Verner-Jeffreys, Richard K Paley, Georgina Rimmer, David Ryder, Patrick Hooper, David Stone, Stephen W Feist
One of the fastest growing fisheries in the UK is the king scallop Pecten maximus L., also currently rated as the second most valuable. Mass mortality events in scallops have been reported world-wide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay Marine Protected Area (MPA), in South West England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies of bacteria (IMC), resembling rickettsia-like organisms (RLOs), often with bacteria released in vascular spaces...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29150323/screening-of-patient-blood-samples-for-babesiosis-using-enzymatic-assays
#18
Shekerah Primus, Lavoisier Akoolo, Samantha Schlachter, Nikhat Parveen
Human babesiosis is an emerging tick-borne disease in the United States and Europe. Transmitted by Ixodes ticks, the causative agent Babesia microti is an intraerythrocytic parasite that causes mild to deadly disease. Transmission of B. microti can also occur by transfusion of infected blood and blood products resulting in transfusion-transmitted babesiosis (TTB), which carries a high risk of fatality. To effectively manage this rise in B. microti infections, better screening tools are needed, which require minimal manipulation of the samples before testing...
November 12, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29150003/crispr-cas9-technology-applications-and-human-disease-modeling
#19
Marta Martinez-Lage, Raúl Torres-Ruiz, Sandra Rodriguez-Perales
The CRISPR/Cas9 system development has revolutionized the field of genome engineering through the efficient creation of targeted breaks in the DNA of almost any organism and cell type, opening an avenue for a wide range of applications in biomedical research and medicine. Apart from gene edition through knock-in or knock-out approaches, CRISPR/Cas9 technology has been used for many other purposes, including regulation of endogenous gene expression, epigenome editing, live-cell imaging of chromosomal loci, edition of RNA and high-throughput screening...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29149600/repair-of-uv-induced-dna-damage-independent-of-nucleotide-excision-repair-is-masked-by-mutyh
#20
Abdelghani Mazouzi, Federica Battistini, Sarah C Moser, Joana Ferreira da Silva, Marc Wiedner, Michel Owusu, Charles-Hugues Lardeau, Anna Ringler, Beatrix Weil, Jürgen Neesen, Modesto Orozco, Stefan Kubicek, Joanna I Loizou
DNA lesions caused by UV damage are thought to be repaired solely by the nucleotide excision repair (NER) pathway in human cells. Patients carrying mutations within genes functioning in this pathway display a range of pathologies, including an increased susceptibility to cancer, premature aging, and neurological defects. There are currently no curative therapies available. Here we performed a high-throughput chemical screen for agents that could alleviate the cellular sensitivity of NER-deficient cells to UV-induced DNA damage...
November 16, 2017: Molecular Cell
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