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https://www.readbyqxmd.com/read/28538202/development-of-nanobiocatalysts-through-the-immobilization-of-pseudomonas-fluorescens-lipase-for-applications-in-efficient-kinetic-resolution-of-racemic-compounds
#1
Bharat P Dwivedee, Jayeeta Bhaumik, Shushil K Rai, Joydev K Laha, Uttam C Banerjee
The present work reports covalent immobilization of Pseudomonas fluorescens lipase (PFL) on functionalized multiwalled carbon nanotubes (MWCNTs) as a nanobiocatalyst (NBC). This nanobiocatalyst facilitates efficient kinetic resolution of (RS)-1-phenylethanol into (S)-1-phenylethanol [C=49.7%, eep=99.5%, ees=98.1% and E value=191.4]. The immobilized preparation (MWCNTs-PFL) showed ten-fold increase in activity, thermal stability upto 80 °C and recyclability (8 cycles). MWCNTs-PFL nanobioconjugate demonstrated better stability and enhanced activity compared to covalently immobilized PFL on other matrices (silver nanoparticles, gold nanoparticles and chitosan beads) used for the study...
May 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28535444/passive-detection-of-pb-in-water-using-rock-phosphate-agarose-beads
#2
Harry M Edenborn, Bret H Howard, James I Sams, Dorothy J Vesper, Sherie L Edenborn
In this study, passive detectors for Pb were prepared by immobilizing powdered rock phosphate in agarose beads. Rock phosphate has been used to treat Pb-contaminated waters and soil by fixing the metal as an insoluble pyromorphite mineral. Under lab conditions, Pb was rapidly adsorbed from aqueous solution by the beads over time, consistent with the acidic dissolution of rock phosphate, the precipitation of pyromorphite within the pore space of the agarose gel matrix, and surface exchange reactions. Net accumulation of Pb occurred when beads were exposed to simulated periodic releases of Pb over time...
April 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28535348/coarse-grained-modeling-of-polyethylene-melts-effect-on-dynamics
#3
Brandon L Peters, K Michael Salerno, Anupriya Agrawal, Dvora Perahia, Gary S Grest
The distinctive viscoelastic behavior of polymers results from a coupled interplay of motion on multiple length and time scales. Capturing the broad time and length scales of polymer motion remains a challenge. Using polyethylene (PE) as a model macromolecule, we construct coarse-grained (CG) models of PE with 3-6 methyl groups per CG bead and probe two critical aspects of the technique: pressure corrections required after iterative Boltzmann inversion (IBI) to generate CG potentials that match the pressure of reference fully-atomistic melt simulations and the transferability of CG potentials across temperatures...
May 23, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28534927/genome-wide-association-study-for-birth-weaning-and-yearling-weight-in-colombian-brahman-cattle
#4
Rodrigo Martínez, Diego Bejarano, Yolanda Gómez, Romain Dasoneville, Ariel Jiménez, Gael Even, Johann Sölkner, Gabor Mészáros
Genotypic and phenotypic data of 1,562 animals were analyzed to find genomic regions that potentially influence the birth weight (BW), weaning weight at seven months of age (WW) and yearling weight (YW) of Colombian Brahman cattle, with genotyping conducted using Illumina Bead chip array with 74,669 SNPs. A Single Step Genomic BLUP (ssGBLP), approach was used to estimate the proportion of variance explained by each marker. Multiple regions scattered across the genome were found to influence weights at different ages, also dependent on the trait component (direct or maternal)...
May 22, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28534926/asymmetric-bead-aggregation-for-microfluidic-immunodetection
#5
Sunggu Kim, Sanghoon Han, Junghoon Lee
We report an asymmetric immunoaggregation assay for rapid, label-free, and sub-picomolar protein detection. Asymmetric immunoaggregated beads (AIBs) are formed when binding occurs between 1 μm magnetic (MG) and 2.8 μm polystyrene (PS) beads coated with specific antibodies for a target antigen. Detection of such aggregation is achieved by optical monitoring of AIBs in a flow under an external magnetic field. AIBs are attracted to the upper surface of the microchannel by a magnetic field and made to slide along the surface by a flow drag force...
May 23, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28534915/quantum-dot-based-sensitive-detection-of-disease-specific-exosome-in-serum
#6
Kseniia Boriachek, Md Nazmul Islam, Vinod Gopalan, Alfred K Lam, Nam-Trung Nguyen, Muhammad J A Shiddiky
Tumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28532064/encapsulation-of-lactobacillus-kefiri-in-alginate-microbeads-using-a-double-novel-aerosol-technique
#7
Christian Demitri, Leonardo Lamanna, Egidio De Benedetto, Fabrizio Damiano, Maria Stella Cappello, Luisa Siculella, Alessandro Sannino
Alginate micro beads containing Lactobacillus kefiri (the principal bacteria present in the kefir probiotic drink) were produced by a novel technique based on dual aerosols spaying of alginate based solution and CaCl2 as cross linking agent. Carboxymethylcellulose (CMC) has been also added to the alginate in order to change the physic-chemical properties (viscosity and permeability) of the microbeads. Calcium alginate and CMC are biopolymers that can be used for developing oral drug-delivery systems. These biopolymers have been reported to show a pH-dependent swelling behaviour...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532049/co-delivery-of-cisplatin-and-doxorubicin-from-calcium-phosphate-beads-matrix-scaffolds-for-osteosarcoma-therapy
#8
Ulrike Hess, Shakiba Shahabi, Laura Treccani, Philipp Streckbein, Christian Heiss, Kurosch Rezwan
Bone substitute materials with a controlled drug release ability can fill cavities caused by the resection of bone tumours and thereby combat any leftover bone cancer cells. The combined release of different cytostatics seems to enhance their toxicity. In this study, calcium phosphate beads and matrix scaffolds are combined for a long-term co-delivery of cis-diamminedichloroplatinum (cisplatin, CDDP) and doxorubicin hydrochloride (DOX) as clinical relevant model drugs. Tricalcium phosphate/alginate beads as additional drug carrier are produced by droplet extrusion with ionotropic gelation and incorporated in scaffold matrix by freeze gelation without sintering...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532029/surfactin-loaded-polyvinyl-alcohol-pva-nanofibers-alters-adhesion-of-listeria-monocytogenes-to-polystyrene
#9
J J Ahire, D D Robertson, A J van Reenen, L M T Dicks
Surfactin-loaded polyvinyl alcohol (PVA) nanofibers were spun using gravity electrospinning. Scanning electron microscopy (SEM) images showed that nanofibers spun with surfactin are free from bead formation and uniform in diameter. The average nanofiber diameters were decreased (273±39nm, 259±39nm and 217±33nm) with increasing levels of surfactin (0.5, 1.0 and 1.5%, w/v) into PVA (10%, w/v). The 10% (w/v) PVA had average fiber diameter of 303±33nm. Atomic force microscopy (AFM) analysis showed that fibers spun with surfactin are not smooth as PVA fibers...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531215/association-of-cord-blood-chemokines-and-other-biomarkers-with-neonatal-complications-following-intrauterine-inflammation
#10
Yoshikazu Otsubo, Kunio Hashimoto, Taro Kanbe, Muneichiro Sumi, Hiroyuki Moriuchi
BACKGROUND: Intrauterine inflammation has been associated with preterm birth and neonatal complications. Few reports have comprehensively investigated multiple cytokine profiles in cord blood and precisely identified surrogate markers for intrauterine inflammation. AIM: To identify the cytokines and surrogate markers associated with intrauterine inflammation and subsequent neonatal complications. PATIENTS AND METHODS: We analyzed cord blood samples from 135 patients admitted to the neonatal intensive care unit at Sasebo City General Hospital...
2017: PloS One
https://www.readbyqxmd.com/read/28530795/modelling-living-cells-response-to-surface-tension-and-chemical-patterns
#11
Marco Macis, Francesca Lugli, Francesco Zerbetto
Mechanobiology is an important epigenetic factor. It influences cell functioning and bears on gene induction, protein synthesis, cell growth and differentiation. In the presence of patterned chemical cues, living cells can take shapes that are far from that of a drop of fluid. These shapes are characterized by inward curvatures that are pinned at the points of location of the cues. The mechano-chemical interactions that orchestrate cell behavior is simulated and controlled by modeling the cells as made by parcels of fluid...
May 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28530538/qualitative-estimation-of-drug-entrapment-efficiency-in-polymeric-nano-micelles-using-dpd
#12
Farzin Hadizadeh, Jamal Shamsara
BACKGROUND: Dissipative particle dynamics (DPD) is a simulation method that one of its applications is in the field of pharmaceutical science and drug delivery. OBJECTIVE: DPD is employed to study morphology and some other characteristics of polymeric nano-micelles. Two systems were considered in this study: system A which includes curcumin, Polycaprolactone (PCL), Polyethylene glycol (PEG) and water beads and system B which includes paclitaxel, Polylactic acid (PLA), PEG and water beads...
May 22, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28530391/single-bead-quantification-of-peptide-loading-distribution-for-one-bead-one-compound-library-synthesis-using-confocal-raman-spectroscopy
#13
Yuchen Tang, Yann Thillier, Ruiwu Liu, Xiaocen Li, Kit S Lam, Tingjuan Gao
We report an analytical method to determine peptide loading of "one-bead one-compound" (OBOC) combinatorial peptide libraries at single-bead level. The quantification is based on a linear relationship between the amount of N-terminal amino groups on individual peptide beads and the intensity of Raman signal obtained from a specifically designed reporter labeled on amino groups. Confocal Raman spectroscopy was employed to characterize peptide loading of beads with defined peptide sequences and from OBOC combinatorial peptide libraries...
May 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28527761/bead-based-flow-cytometry-for-semi-quantitative-analysis-of-complex-membrane-vesicle-populations-released-by-bacteria-and-host-cells
#14
Charlotte Volgers, Birke J Benedikter, Gert E Grauls, Paul H M Savelkoul, Frank R M Stassen
During infection, the release of nano-sized membrane vesicle is a process which is common both for bacteria and host cells. Host cell-derived membrane vesicles can be involved in innate and adaptive immunity whereas bacterial membrane vesicles can contribute to bacterial pathogenicity. To study the contribution of both membrane vesicle populations during infection is highly complicated as most vesicles fall within a similar size range of 30-300nm. Specialized techniques for purification are required and often no single technique complies on its own...
July 2017: Microbiological Research
https://www.readbyqxmd.com/read/28526413/tcr-crosslinking-promotes-crk-adaptor-protein-binding-to-tyrosine-phosphorylated-cd3%C3%AE-chain
#15
Guangyu Dong, Rachel Kalifa, Pulak Ranjan Nath, Sigal Gelkop, Noah Isakov
T cell antigen receptor (TCR) binding of a peptide antigen presented by antigen-presenting cells (APCs) in the context of surface MHC molecules initiates signaling events that regulate T cell activation, proliferation and differentiation. A key event in the activation process is the phosphorylation of the conserved tyrosine residues within the CD3 chain immunoreceptor tyrosine-based activation motifs (ITAMs), which operate as docking sites for SH2 domain-containing effector proteins. Phosphorylation of the CD3ζ ITAMs renders the CD3 chain capable of binding the ζ-chain associated protein 70 kDa (ZAP70), a protein tyrosine kinase that is essential for T cell activation...
May 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28526345/facile-fabrication-of-3d-porous-hybrid-sphere-by-co-immobilization-of-multi-enzyme-directly-from-cell-lysates-as-an-efficient-and-recyclable-biocatalyst-for-asymmetric-reduction-with-coenzyme-regeneration-in-situ
#16
MinganYu, Duqiang Liu, Lili Sun, Jing Li, Qian Chen, Lijun Pan, Jingchuan Shang, Shurong Zhang, Wei Li
Ni(2+)-agarose bead-wrapped multi-enzyme/inorganic hybrid sphere composed of the immobilized enzymes as organic component and NaH2PO4 and NaCl as inorganic component was developed by co-immobilizing extracellular His-tagged 3-quinuclidinone reductases and glucose dehydrogenase without pre-purification. The resulting biocatalysts has 3D porous architectures as confirmed by SEM and FESEM, and it enabled the continuous biotransformation of 3-quinuclidone to (R)-3-quinuclidinol with cofactor regeneration in situ...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28525851/a-fully-integrated-distance-readout-elisa-chip-for-point-of-care-testing-with-sample-in-answer-out-capability
#17
Dan Liu, Xingrui Li, Junkai Zhou, Shibo Liu, Tian Tian, Yanling Song, Zhi Zhu, Leiji Zhou, Tianhai Ji, Chaoyong Yang
Enzyme-linked immunosorbent assay (ELISA) is a popular laboratory technique for detection of disease-specific protein biomarkers with high specificity and sensitivity. However, ELISA requires labor-intensive and time-consuming procedures with skilled operators and spectroscopic instrumentation. Simplification of the procedures and miniaturization of the devices are crucial for ELISA-based point-of-care (POC) testing in resource-limited settings. Here, we present a fully integrated, instrument-free, low-cost and portable POC platform which integrates the process of ELISA and the distance readout into a single microfluidic chip...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525313/a-tunable-three-dimensional-in-vitro-culture-model-of-growth-plate-cartilage-using-alginate-hydrogel-scaffolds
#18
Alek G Erickson, Taylor D Laughlin, Sarah M Romereim, Catherine N Sargus-Patino, Angela K Pannier, Andrew T Dudley
Defining the final size and geometry of engineered tissues through precise control of the scalar and vector components of tissue growth is a necessary benchmark for regenerative medicine, but it has proved to be a significant challenge for tissue engineers. The growth plate cartilage that promotes elongation of the long bones is a good model system for studying morphogenetic mechanisms because cartilage is composed of a single cell type, the chondrocyte; chondrocytes are readily maintained in culture; and growth trajectory is predominately in a single vector...
May 18, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28523920/expression-of-bovine-rotavirus-vp8-and-preparation-of-igy-antibodies-against-recombinant-vp8
#19
H S Kim, B Lee, S Y Han, Y T Jung
Group A rotavirus is the leading cause of acute gastroenteritis in cattle and swine. Although, vaccination against this virus is an effective strategy for prevention, additional strategy to control disease is necessary. Egg yolk immunoglobulin (IgY)-based passive immunization could be a better option in preventing this disease. Bovine rotavirus (BRV) is group A rotavirus and possesses a genome of 11 segments of double-stranded RNA. The outer layer of capsid is composed of two proteins (VP7 and VP4), which induce virus neutralizing antibodies...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28523725/differential-attenuation-of-nmr-signals-by-complementary-ion-exchange-resin-beads-for-de-novo-analysis-of-complex-metabolomics-mixtures
#20
Jiaqi Yuan, Bo Zhang, Rafael Bruschweiler
A primary goal of metabolomics is the characterization of a potentially very large number of metabolites that are part of complex mixtures. Application to biofluids and tissue samples offers insights into biochemical metabolic pathways and their role in health and disease. 1D 1H and 2D 13C-1H HSQC NMR spectra are most commonly used for this purpose. They yield quantitative information about each proton of the mixture, but not which protons belong to the same molecule. Interpretation requires the use of NMR spectral databases naturally limited to known metabolites...
May 18, 2017: Chemistry: a European Journal
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