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https://www.readbyqxmd.com/read/28821029/effect-of-gum-arabic-modified-alginate-on-physicochemical-properties-release-kinetics-and-storage-stability-of-liquid-core-hydrogel-beads
#1
Fu-Hsuan Tsai, Yutaka Kitamura, Mito Kokawa
Different weight ratios of alginate/gum arabic (GA) solutions were prepared to serve as the wall material of liquid-core hydrogel beads (LHB) that were formulated to protect the total phenolic compounds (TP) of radish by-product juice from degradation during storage and release in simulated gastrointestinal fluid. The diameter of LHB ranged from 4.63 to 5.66mm with a sphericity lower than 0.05. LHB formulated with 25% GA (AGB0.25) exhibited the highest hardness (26.63N), and those formulated with 50% GA (AGB0...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820263/hydrodynamic-steering-in-protein-association-revisited-surprisingly-minuscule-effects-of-considerable-torques
#2
Jan Marek Antosiewicz, Kamil Kamiński, Maciej Dlugosz
We investigate the previously postulated hydrodynamic steering phenomenon, resulting from complication of molecular shapes, its magnitude and possible relevance for protein-ligand and protein-protein diffusional encounters, and the kinetics of diffusion-controlled association. We consider effects of hydrodynamic interactions in a prototypical model system consisting of a cleft enzyme and an elongated substrate, and real protein-protein complexes, that of barnase and barstar, and human growth hormone and its binding protein...
August 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28819334/cytokine-signature-in-end-stage-renal-disease-patients-on-hemodialysis
#3
Danyelle Romana Alves Rios, Melina Barros Pinheiro, Wander Valadares de Oliveira Junior, Karina Braga Gomes, Andréa Teixeira Carvalho, Olindo Assis Martins-Filho, Ana Cristina Simões E Silva, Luci Maria Sant'Ana Dusse
Hemodialysis is a modality of blood filtration in which accumulated toxins and water are removed from the body. This treatment is indicated for patients at the end stage of renal disease. Vascular access complications are responsible for 20-25% of all hospitalizations in dialyzed patients. The occurrence of thrombosis in the vascular access is a serious problem that may severely compromise or even make the hemodialysis impossible, which is vital for the patient. The aim of this study was to investigate inflammatory profile in patients undergoing hemodialysis as well as the association between these alterations and vascular access thrombosis...
2017: Disease Markers
https://www.readbyqxmd.com/read/28819310/genome-wide-target-enrichment-aided-chip-design-a-66%C3%A2-k-snp-chip-for-cashmere-goat
#4
Xian Qiao, Rui Su, Yang Wang, Ruijun Wang, Ting Yang, Xiaokai Li, Wei Chen, Shiyang He, Yu Jiang, Qiwu Xu, Wenting Wan, Yaolei Zhang, Wenguang Zhang, Jiang Chen, Bin Liu, Xin Liu, Yixing Fan, Duoyuan Chen, Huaizhi Jiang, Dongming Fang, Zhihong Liu, Xiaowen Wang, Yanjun Zhang, Danqing Mao, Zhiying Wang, Ran Di, Qianjun Zhao, Tao Zhong, Huanming Yang, Jian Wang, Wen Wang, Yang Dong, Xiaoli Chen, Xun Xu, Jinquan Li
Compared with the commercially available single nucleotide polymorphism (SNP) chip based on the Bead Chip technology, the solution hybrid selection (SHS)-based target enrichment SNP chip is not only design-flexible, but also cost-effective for genotype sequencing. In this study, we propose to design an animal SNP chip using the SHS-based target enrichment strategy for the first time. As an update to the international collaboration on goat research, a 66 K SNP chip for cashmere goat was created from the whole-genome sequencing data of 73 individuals...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818721/immunomodulatory-effects-of-herbal-formula-of-astragalus-polysaccharide-aps-and-polysaccharopeptide-psp-in-mice-with-lung-cancer
#5
Xing Zhou, Zijing Liu, Tingting Long, Lijng Zhou, Yixi Bao
OBJECTIVE: This study is to investigate the immunomodulatory effects of the herbal formula of astragalus polysaccharide (APS) and polysaccharopeptide (PSP) in mouse models of immunosuppression and lung cancer. METHODS: Immune parameters were recorded for these model mice. Peripheral white blood cells (WBC) were detected with the automatic blood cell analyzer. Spleen and thymus indices, and tumor inhibition ratio were obtained. Percentage of peripheral blood CD4(+) and CD8(+) T lymphocytes were detected by flow cytometry...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28818598/serotype-determination-of-salmonella-by-xtag-assay
#6
Zhibei Zheng, Wei Zheng, Haoqiu Wang, Jincao Pan, Xiaoying Pu
Currently, no protocols or commercial kits are available to determine the serotypes of Salmonella by using Luminex MAGPIX®. In this study, an xTAG assay for serotype determination of Salmonella suitable for Luminex MAGPIX® is described and 228 Salmonella isolates were serotype determined by this xTAG assay. The xTAG assay consists of two steps: 1) Multiplex PCR to amplify simultaneously O, H and Vi antigen genes of Salmonella, and 2) Magplex-TAG™ microsphere hybridization to identify accurately the specific PCR products of different antigens...
August 14, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28817608/data-driven-coarse-graining-of-large-biomolecular-structures
#7
Yi-Ling Chen, Michael Habeck
Advances in experimental and computational techniques allow us to study the structure and dynamics of large biomolecular assemblies at increasingly higher resolution. However, with increasing structural detail it can be challenging to unravel the mechanism underlying the function of molecular machines. One reason is that atomistic simulations become computationally prohibitive. Moreover it is difficult to rationalize the functional mechanism of systems composed of tens of thousands to millions of atoms by following each atom's movements...
2017: PloS One
https://www.readbyqxmd.com/read/28817270/magnetic-field-activated-sensing-of-mrna-in-living-cells
#8
Saira F Bakshi, Nataliia Guz, Andrey Zakharchenko, Han Deng, Alexei Tumanov, Craig D Woodworth, Sergiy Minko, Dmitry M Kolpashchikov, Evgeny Katz
Detection of specific mRNA in living cells has attracted significant attention in the last decade. Probes that can be easily delivered into cells and activated at the desired time can contribute to understand-ing translation, trafficking and degradation of mRNA. Here we report a new strategy termed Magnetic Field-Activated Binary De-oxyribozyme (MaBiDZ) sensor that enables both efficient delivery and temporal control of mRNA sensing by magnetic field. MaBiDZ uses two species of magnetic beads conjugated with different com-ponents of a multicomponent deoxyribozyme (DZ) sensor...
August 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28816436/integrated-distance-based-origami-paper-analytical-device-id-opad-for-one-step-visualized-analysis
#9
Tian Tian, Yuan An, Yiping Wu, Yanling Song, Zhi Zhu, Chaoyong James Yang
An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user-friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification and visual signal output, based on aptamer/invertase functionalized sepharose beads, cascaded enzymatic reactions and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, while sepharose beads with larger size are excluded and stay in the upper zone...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28816177/electrical-stimulation-of-gut-motility-guided-by-an-in-silico-model
#10
Bradley Brigham Barth, Craig S Henriquez, Warren M Grill, Xiling Shen
OBJECTIVE: Neuromodulation of the central and peripheral nervous systems is becoming increasingly important for treating a diverse set of diseases-ranging from Parkinson's Disease and epilepsy to chronic pain. However, neuromodulation of the gastrointestinal (GI) tract has achieved relatively limited success in treating functional GI disorders, which affect a significant population, because the effects of stimulation on the enteric nervous system (ENS) and gut motility are not well understood...
August 17, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28813434/detection-of-anti-hspx-antibodies-and-hspx-protein-in-patient-sera-for-the-identification-of-recent-latent-infection-by-mycobacterium-tuberculosis
#11
Jorge Castro-Garza, Paola García-Jacobo, Lydia G Rivera-Morales, Frederick D Quinn, James Barber, Russell Karls, Debra Haas, Shelly Helms, Tuhina Gupta, Henry Blumberg, Jane Tapia, Itza Luna-Cruz, Adrián Rendon, Javier Vargas-Villarreal, Lucio Vera-Cabrera, Cristina Rodríguez-Padilla
Mycobacterium tuberculosis is a pathogen causing tuberculosis (TB) a spectrum of disease including acute and asymptomatic latent stages. Identifying and treating latently-infected patients constitutes one of the most important impediments to TB control efforts. Those individuals can remain undiagnosed for decades serving as potential reservoirs for disease reactivation. Tests for the accurate diagnosis of latent infection currently are unavailable. HspX protein (α-crystallin), encoded by Rv2031c gene, is produced in vitro by M...
2017: PloS One
https://www.readbyqxmd.com/read/28812231/the-neuroprotective-effect-of-conditioned-medium-from-human-adipose-derived-mesenchymal-stem-cells-is-impaired-by-n-acetyl-cysteine-supplementation
#12
Teodoro Palomares, María Cordero, Cristina Bruzos-Cidon, María Torrecilla, Luisa Ugedo, Ana Alonso-Varona
Oxidative stress is a common feature in neurodegenerative diseases associated with neuroinflammation, and therefore, has been proposed as a key target for novel therapies for these diseases. Recently, adipose-derived stem cell (ASC)-based cell therapy has emerged as a novel strategy for neuroprotection. In this study, we evaluate the therapeutic role of ASC-conditioned medium (ASC-CM) against H2O2-induced neurotoxicity in a new in vitro model of ec23/brain-derived neurotrophic factor (BDNF)-differentiated human SH-SY5Y neuron-like cells (SH-SY5Yd)...
August 15, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28812210/increased-il-6-expression-on-thp-1-by-il-34-stimulation-up-regulated-rheumatoid-arthritis-th17-cells
#13
Bing Wang, Yawei Tang, Xiaotong Sun, Xunli Ouyang, Han Li, Jing Wei, Yan Zhang, Xia Li
IL-34 is a pleiotropic cytokine, which is a key regulator of monocytes/macrophages and might participate in the pathogenesis of RA. In this study, we aimed to explore the effect of IL-34 on the monocyte-like cell line THP-1 and the quantitative variation of Th17 cells in THP-1 and RA CD4(+)T cells coculture system. CD4(+)T cells were purified from RA PBMC using immunomagnetic beads. THP-1 were cultured with RA CD4(+)T cells. The frequency of Th17 cells was determined by FACS. Fluorscence indensity and expression of ROS were detected by FACS and cell staining, respectively...
August 15, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28810734/adsorption-and-depletion-regimes-of-a-nonionic-surfactant-in-hydrophilic-mesopores-an-experimental-and-simulation-study
#14
Dirk Müter, Gernot Rother, Henry Bock, Martin Schoen, Gerhard H Findenegg
Adsorption and aggregation of nonionic surfactants at oxide surfaces has been studied extensively in the past, but only for concentrations below and near the critical micelle concentration. Here we report an adsorption study of a short-chain surfactant (C6E3) in porous silica glass of different pore sizes (7.5 to 50 nm), covering a wide composition range up to 50 wt.-% in a temperature range from 20°C to the LCST. Aggregative adsorption is observed at low concentrations, but the excess concentration of C6E3 in the pores decreases and approaches zero at higher bulk concentrations...
August 15, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28809925/salivary-peptidome-profiling-analysis-for-occurrence-of-new-carious-lesions-in-patients-with-severe-early-childhood-caries
#15
Chang Tian, Xiangyu Sun, Xiaochen Liu, Xin Huang, Feng Chen, Shuguo Zheng
This study aimed to identify differences of peptide profiles in stimulated whole saliva among children with and without occurrence of new carious lesions, and to provide a simple way for early diagnosis and prevention of the relapse of severe early childhood caries (s-ECC). Overall, 26 children aged 3-4 years were selected out from all the children in the kindergarten to be involved in the present study, among them 13 were diagnosed as s-ECC and underwent dental treatment, whilst the other 13 were matched by age and sex as control...
2017: PloS One
https://www.readbyqxmd.com/read/28809773/a-macaque-model-for-rectal-lymphogranuloma-venereum-and-non-lymphogranuloma-venereum-chlamydia-trachomatis-impact-on-rectal-simian-human-immunodeficiency-virus-acquisition
#16
Sundaram Ajay Vishwanathan, Rachael D Aubert, Monica R Morris, Chunxia Zhao, Christi Philips, George M Khalil, Frank Deyounks, Kristen Kelley, Jana M Ritter, C Y Chen, Ellen N Kersh, Janet M McNicholl
BACKGROUND: Sustained genital tract inflammation caused by sexually transmitted infections (STIs) is known to increase risk of vaginal human immunodeficiency virus (HIV) infections but, to our knowledge, there are no nonhuman primate studies that have evaluated its link to rectal HIV acquisition. METHODS: Rhesus macaques inoculated with Chlamydia trachomatis (CT) (serovars LGV-L2 and CT-E; n = 7) or saline (n = 7) received up to 20 rectal challenges twice a week of simian/HIV immunodeficiency virus (SHIVSF162p3)...
September 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28808997/target-identification-using-cell-permeable-and-cleavable-chloroalkane-derivatized-small-molecules
#17
Jacqui L Mendez-Johnson, Danette L Daniels, Marjeta Urh, Rachel Friedman Ohana
An important aspect for gaining functional insight into the activity of small molecules revealed through phenotypic screening is the identification of their interacting proteins. Yet, isolating and validating these interacting proteins remains difficult. Here, we present a new approach utilizing a chloroalkane (CA) moiety capture handle, which can be chemically attached to small molecules to isolate their respective protein targets. Derivatization of small molecules with the CA moiety has been shown to not significantly impact their cell permeability or potency, allowing for phenotypic validation of the derivatized small molecule prior to capture...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28808837/a-multiparticulate-delivery-system-for-potential-colonic-targeting-using-bovine-serum-albumin-as-a-model-protein
#18
Bowen Jiang, Hua Yu, Yongrong Zhang, Hanping Feng, Stephen W Hoag
PURPOSE: There are many important diseases whose treatment could be improved by delivering a therapeutic protein to the colon, for example, Clostridium difficile infection, ulcerative colitis and Crohn's Disease. The goal of this project was to investigate the feasibility of colonic delivery of proteins using multiparticulate beads. METHODS: In this work, bovine serum albumin (BSA) was adopted as a model protein. BSA was spray layered onto beads, followed by coating of an enteric polymer EUDRAGIT® FS 30 D to develop a colonic delivery system...
August 14, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28808438/atsas-2-8-a-comprehensive-data-analysis-suite-for-small-angle-scattering-from-macromolecular-solutions
#19
D Franke, M V Petoukhov, P V Konarev, A Panjkovich, A Tuukkanen, H D T Mertens, A G Kikhney, N R Hajizadeh, J M Franklin, C M Jeffries, D I Svergun
ATSAS is a comprehensive software suite for the analysis of small-angle scattering data from dilute solutions of biological macromolecules or nanoparticles. It contains applications for primary data processing and assessment, ab initio bead modelling, and model validation, as well as methods for the analysis of flexibility and mixtures. In addition, approaches are supported that utilize information from X-ray crystallography, nuclear magnetic resonance spectroscopy or atomistic homology modelling to construct hybrid models based on the scattering data...
August 1, 2017: Journal of Applied Crystallography
https://www.readbyqxmd.com/read/28808159/chronic-brucella-infection-induces-selective-and-persistent-ifn-%C3%AE-dependent-alterations-of-marginal-zone-macrophages-in-the-spleen
#20
Arnaud Machelart, Abir Khadrawi, Aurore Demars, Kevin Willemart, Carl De Trez, Jean-Jacques Letesson, Eric Muraille
The spleen is known as an important filter for blood-borne pathogens that are trapped by specialized macrophages in the marginal zone (MZ): the CD209(+) MZ macrophages (MZMs) and the CD169(+) marginal metallophilic macrophages (MMMs). Acute systemic infection strongly impacts MZ populations and the location of T and B lymphocytes. This phenomenon has been linked to reduced chemokine secretion by stromal cells. Brucella spp. are the causative agent of brucellosis, a widespread zoonotic disease. Here, we used B...
August 14, 2017: Infection and Immunity
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