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Cornelia bargmann

Christine E Cho, Chantal Brueggemann, Noelle D L'Etoile, Cornelia I Bargmann
Sensory experience modifies behavior through both associative and non-associative learning. In Caenorhabditis elegans, pairing odor with food deprivation results in aversive olfactory learning, and pairing odor with food results in appetitive learning. Aversive learning requires nuclear translocation of the cGMP-dependent protein kinase EGL-4 in AWC olfactory neurons and an insulin signal from AIA interneurons. Here we show that the activity of neurons including AIA is acutely required during aversive, but not appetitive, learning...
2016: ELife
Sara Abrahamsson, Rob Ilic, Jan Wisniewski, Brian Mehl, Liya Yu, Lei Chen, Marcelo Davanco, Laura Oudjedi, Jean-Bernard Fiche, Bassam Hajj, Xin Jin, Joan Pulupa, Christine Cho, Mustafa Mir, Mohamed El Beheiry, Xavier Darzacq, Marcelo Nollmann, Maxime Dahan, Carl Wu, Timothée Lionnet, J Alexander Liddle, Cornelia I Bargmann
Multifocus microscopy (MFM) allows high-resolution instantaneous three-dimensional (3D) imaging and has been applied to study biological specimens ranging from single molecules inside cells nuclei to entire embryos. We here describe pattern designs and nanofabrication methods for diffractive optics that optimize the light-efficiency of the central optical component of MFM: the diffractive multifocus grating (MFG). We also implement a "precise color" MFM layout with MFGs tailored to individual fluorophores in separate optical arms...
March 1, 2016: Biomedical Optics Express
Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I Bargmann, Misha B Ahrens, Eric R Schreiter, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity...
2016: ELife
Xin Jin, Navin Pokala, Cornelia I Bargmann
Memories formed early in life are particularly stable and influential, representing privileged experiences that shape enduring behaviors. We show that exposing newly hatched C. elegans to pathogenic bacteria results in persistent aversion to those bacterial odors, whereas adult exposure generates only transient aversive memory. Long-lasting imprinted aversion has a critical period in the first larval stage and is specific to the experienced pathogen. Distinct groups of neurons are required during formation (AIB, RIM) and retrieval (AIY, RIA) of the imprinted memory...
February 11, 2016: Cell
William M Roberts, Steven B Augustine, Kristy J Lawton, Theodore H Lindsay, Tod R Thiele, Eduardo J Izquierdo, Serge Faumont, Rebecca A Lindsay, Matthew Cale Britton, Navin Pokala, Cornelia I Bargmann, Shawn R Lockery
Random search is a behavioral strategy used by organisms from bacteria to humans to locate food that is randomly distributed and undetectable at a distance. We investigated this behavior in the nematode Caenorhabditis elegans, an organism with a small, well-described nervous system. Here we formulate a mathematical model of random search abstracted from the C. elegans connectome and fit to a large-scale kinematic analysis of C. elegans behavior at submicron resolution. The model predicts behavioral effects of neuronal ablations and genetic perturbations, as well as unexpected aspects of wild type behavior...
January 29, 2016: ELife
Sreekanth H Chalasani, Nikos Chronis, Makoto Tsunozaki, Jesse M Gray, Daniel Ramot, Miriam B Goodman, Cornelia I Bargmann
No abstract text is available yet for this article.
May 5, 2016: Nature
Johannes Larsch, Steven W Flavell, Qiang Liu, Andrew Gordus, Dirk R Albrecht, Cornelia I Bargmann
Animals have a remarkable ability to track dynamic sensory information. For example, the nematode Caenorhabditis elegans can locate a diacetyl odor source across a 100,000-fold concentration range. Here, we relate neuronal properties, circuit implementation, and behavioral strategies underlying this robust navigation. Diacetyl responses in AWA olfactory neurons are concentration and history dependent; AWA integrates over time at low odor concentrations, but as concentrations rise, it desensitizes rapidly through a process requiring cilia transport...
September 22, 2015: Cell Reports
Cornelia I Bargmann
No abstract text is available yet for this article.
July 21, 2015: JAMA: the Journal of the American Medical Association
Sara Abrahamsson, Molly McQuilken, Shalin B Mehta, Amitabh Verma, Johannes Larsch, Rob Ilic, Rainer Heintzmann, Cornelia I Bargmann, Amy S Gladfelter, Rudolf Oldenbourg
We have developed an imaging system for 3D time-lapse polarization microscopy of living biological samples. Polarization imaging reveals the position, alignment and orientation of submicroscopic features in label-free as well as fluorescently labeled specimens. Optical anisotropies are calculated from a series of images where the sample is illuminated by light of different polarization states. Due to the number of images necessary to collect both multiple polarization states and multiple focal planes, 3D polarization imaging is most often prohibitively slow...
March 23, 2015: Optics Express
Lyric A Jorgenson, William T Newsome, David J Anderson, Cornelia I Bargmann, Emery N Brown, Karl Deisseroth, John P Donoghue, Kathy L Hudson, Geoffrey S F Ling, Peter R MacLeish, Eve Marder, Richard A Normann, Joshua R Sanes, Mark J Schnitzer, Terrence J Sejnowski, David W Tank, Roger Y Tsien, Kamil Ugurbil, John C Wingfield
The evolution of the field of neuroscience has been propelled by the advent of novel technological capabilities, and the pace at which these capabilities are being developed has accelerated dramatically in the past decade. Capitalizing on this momentum, the United States launched the Brain Research through Advancing Innovative Neurotechnologies (BRAIN) Initiative to develop and apply new tools and technologies for revolutionizing our understanding of the brain. In this article, we review the scientific vision for this initiative set forth by the National Institutes of Health and discuss its implications for the future of neuroscience research...
May 19, 2015: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
Andrew Gordus, Navin Pokala, Sagi Levy, Steven W Flavell, Cornelia I Bargmann
Variability is a prominent feature of behavior and is an active element of certain behavioral strategies. To understand how neuronal circuits control variability, we examined the propagation of sensory information in a chemotaxis circuit of C. elegans where discrete sensory inputs can drive a probabilistic behavioral response. Olfactory neurons respond to odor stimuli with rapid and reliable changes in activity, but downstream AIB interneurons respond with a probabilistic delay. The interneuron response to odor depends on the collective activity of multiple neurons-AIB, RIM, and AVA-when the odor stimulus arrives...
April 9, 2015: Cell
Cornelia I Bargmann, Jeffrey A Lieberman
No abstract text is available yet for this article.
October 2014: American Journal of Psychiatry
Cornelia I Bargmann, William T Newsome
No abstract text is available yet for this article.
June 2014: JAMA Neurology
Navin Pokala, Qiang Liu, Andrew Gordus, Cornelia I Bargmann
Recent progress in neuroscience has been facilitated by tools for neuronal activation and inactivation that are orthogonal to endogenous signaling systems. We describe here a chemical-genetic approach for inducible silencing of Caenorhabditis elegans neurons in intact animals, using the histamine-gated chloride channel HisCl1 from Drosophila and exogenous histamine. Administering histamine to freely moving C. elegans that express HisCl1 transgenes in neurons leads to rapid and potent inhibition of neural activity within minutes, as assessed by behavior, functional calcium imaging, and electrophysiology of neurons expressing HisCl1...
February 18, 2014: Proceedings of the National Academy of Sciences of the United States of America
Saul Kato, Yifan Xu, Christine E Cho, L F Abbott, Cornelia I Bargmann
Animals track fluctuating stimuli over multiple timescales during natural olfactory behaviors. Here, we define mechanisms underlying these computations in Caenorhabditis elegans. By characterizing neuronal calcium responses to rapidly fluctuating odor sequences, we show that sensory neurons reliably track stimulus fluctuations relevant to behavior. AWC olfactory neurons respond to multiple odors with subsecond precision required for chemotaxis, whereas ASH nociceptive neurons integrate noxious cues over several seconds to reach a threshold for avoidance behavior...
February 5, 2014: Neuron
Elizabeth E Glater, Matthew V Rockman, Cornelia I Bargmann
The nematode Caenorhabditis elegans can use olfaction to discriminate among different kinds of bacteria, its major food source. We asked how natural genetic variation contributes to choice behavior, focusing on differences in olfactory preference behavior between two wild-type C. elegans strains. The laboratory strain N2 strongly prefers the odor of Serratia marcescens, a soil bacterium that is pathogenic to C. elegans, to the odor of Escherichia coli, a commonly used laboratory food source. The divergent Hawaiian strain CB4856 has a weaker attraction to Serratia than the N2 strain, and this behavioral difference has a complex genetic basis...
February 2014: G3: Genes—Genomes—Genetics
Johannes Larsch, Donovan Ventimiglia, Cornelia I Bargmann, Dirk R Albrecht
Neuronal responses to sensory inputs can vary based on genotype, development, experience, or stochastic factors. Existing neuronal recording techniques examine a single animal at a time, limiting understanding of the variability and range of potential responses. To scale up neuronal recordings, we here describe a system for simultaneous wide-field imaging of neuronal calcium activity from at least 20 Caenorhabditis elegans animals under precise microfluidic chemical stimulation. This increased experimental throughput was used to perform a systematic characterization of chemosensory neuron responses to multiple odors, odor concentrations, and temporal patterns, as well as responses to pharmacological manipulation...
November 5, 2013: Proceedings of the National Academy of Sciences of the United States of America
Heeun Jang, Cornelia I Bargmann
The pheromone drop test is a simple and robust behavioral assay to quantify acute avoidance of pheromones in C. elegans, and the suppression of avoidance by attractive pheromones. In the pheromone drop test, water-soluble C. elegans pheromones are individually applied to animals that are freely moving on a large plate. Upon encountering a repellent, each C. elegans animal may or may not try to escape by making a long reversal. The fraction of animals that make a long reversal response indicates the repulsiveness of a given pheromone to a specific genotype/strain of C...
2013: Methods in Molecular Biology
Cornelia Bargmann
Cori Bargmann's studies of olfaction and her work on the connections between neural circuits, genes, and behavior caught our attention. Curious to know more, BioTechniques contacted her to find out about the ambition, character, and motivation that led to her success.
July 2013: BioTechniques
Steven W Flavell, Navin Pokala, Evan Z Macosko, Dirk R Albrecht, Johannes Larsch, Cornelia I Bargmann
Foraging animals have distinct exploration and exploitation behaviors that are organized into discrete behavioral states. Here, we characterize a neuromodulatory circuit that generates long-lasting roaming and dwelling states in Caenorhabditis elegans. We find that two opposing neuromodulators, serotonin and the neuropeptide pigment dispersing factor (PDF), each initiate and extend one behavioral state. Serotonin promotes dwelling states through the MOD-1 serotonin-gated chloride channel. The spontaneous activity of serotonergic neurons correlates with dwelling behavior, and optogenetic modulation of the critical MOD-1-expressing targets induces prolonged dwelling states...
August 29, 2013: Cell
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