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https://www.readbyqxmd.com/read/29325714/aging-related-sumoylation-pattern-in-the-cortex-and-blood-plasma-of-wild-type-mice
#1
E Ficulle, M D Shah Sufian, C Tinelli, M Corbo, M Feligioni
Protein activities and mechanisms related to aging has become a growing interest nowadays. Since SUMOylation is implicated in several cellular processes, its investigation related to senescence, aging and frailty is of high interest. In our study, wild type mice cortical lysates, synaptosomes and plasma have been processed to evaluate SUMOylation and SUMO machinery expression (Ubc9 and SENP1 enzymes) profile at different ages. In cortical lysates, SUMO-1ylation reached a peak at 6 months followed by a decrease; while in synaptosomes, it progressively increased till 18 months...
January 8, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29299635/both-conditional-ablation-and-overexpression-of-e2-sumo-conjugating-enzyme-ubc9-in-mouse-pancreatic-beta-cells-result-in-impaired-beta-cell-function
#2
Xiaoyu He, Qiaohong Lai, Cai Chen, Na Li, Fei Sun, Wenting Huang, Shu Zhang, Qilin Yu, Ping Yang, Fei Xiong, Zhishui Chen, Quan Gong, Boxu Ren, Jianping Weng, Décio L Eizirik, Zhiguang Zhou, Cong-Yi Wang
AIMS/HYPOTHESIS: Post-translational attachment of a small ubiquitin-like modifier (SUMO) to the lysine (K) residue(s) of target proteins (SUMOylation) is an evolutionary conserved regulatory mechanism. This modification has previously been demonstrated to be implicated in the control of a remarkably versatile regulatory mechanism of cellular processes. However, the exact regulatory role and biological actions of the E2 SUMO-conjugating enzyme (UBC9)-mediated SUMOylation function in pancreatic beta cells has remained elusive...
January 3, 2018: Diabetologia
https://www.readbyqxmd.com/read/29275599/-hypoxia-inducible-factor-1%C3%AE-desumoylation-reduces-the-stemness-maintenance-ability-of-endometrial-cancer-stem-cell-and-increases-its-chemosensitivity
#3
L Yuan, Z M Jiang, X H Chen, X Y Bian, Y X Li, X F Ma, X Z Liu
Objective: To reduce the stemness maintenance ability of endometrial cancer stem cell and increase its sensitivity to chemotherapy by inducing hypoxia inducible factor 1α (HIF-1α) protein deSUMOylation. Methods: Lentiviral plasmid mediated ubiquitin carrier protein 9 (Ubc9) gene silencing was transgened into KLE endometrial carcinoma cells. The expression of Ubc9, small ubiquitin-related modifier 1(SUMO1) and HIF-1α protein was detected by Western blotting. Then tumor stem cells clones were cultured in 96 well plates, and these clone balls diameter were calculated...
December 5, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29236778/interaction-of-porcine-reproductive-and-respiratory-syndrome-virus-proteins-with-sumo-conjugating-enzyme-reveals-the-sumoylation-of-nucleocapsid-protein
#4
Cong Wang, Nanfang Zeng, Siyu Liu, Qi Miao, Lei Zhou, Xinna Ge, Jun Han, Xin Guo, Hanchun Yang
SUMOylation is a reversible post-translational modification that regulates the function of target protein. In this study, we first predicted by software that the multiple proteins of porcine reproductive and respiratory syndrome virus (PRRSV) could be sumoylated. Next, we confirmed that Nsp1β, Nsp4, Nsp9, Nsp10 and nucleocapsid (N) protein of PRRSV could interact with the sole SUMO E2 conjugating enzyme Ubc9, and Ubc9 could be co-localized with Nsp1β, Nsp4, Nsp9 and Nsp10 in the cytoplasm, while with N protein in both the cytoplasm and nucleus...
2017: PloS One
https://www.readbyqxmd.com/read/29234076/molecular-mechanism-of-k65-acetylation-induced-attenuation-of-ubc9-and-the-ndsm-interaction
#5
Mandar T Naik, Mooseok Kang, Chun-Chen Ho, Pei-Hsin Liao, Yung-Lin Hsieh, Nandita M Naik, Szu-Huan Wang, Iksoo Chang, Hsiu-Ming Shih, Tai-Huang Huang
The negatively charged amino acid-dependent sumoylation motif (NDSM) carries an additional stretch of acidic residues downstream of the consensus Ψ-K-x-E/D sumoylation motif. We have previously shown that acetylation of the SUMO E2 conjugase enzyme, Ubc9, at K65 downregulates its binding to the NDSM and renders a selective decrease in sumoylation of substrates with the NDSM motif. Here, we provide detailed structural, thermodynamic, and kinetics results of the interactions between Ubc9 and its K65 acetylated variant (Ac-Ubc9K65) with three NDSMs derived from Elk1, CBP, and Calpain2 to rationalize the mechanism beneath this reduced binding...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29180619/the-stubl-rnf4-regulates-protein-group-sumoylation-by-targeting-the-sumo-conjugation-machinery
#6
Ramesh Kumar, Román González-Prieto, Zhenyu Xiao, Matty Verlaan-de Vries, Alfred C O Vertegaal
SUMO-targeted ubiquitin ligases (STUbLs) mediate the ubiquitylation of SUMOylated proteins to modulate their functions. In search of direct targets for the STUbL RNF4, we have developed TULIP (targets for ubiquitin ligases identified by proteomics) to covalently trap targets for ubiquitin E3 ligases. TULIP methodology could be widely employed to delineate E3 substrate wiring. Here we report that the single SUMO E2 Ubc9 and the SUMO E3 ligases PIAS1, PIAS2, PIAS3, ZNF451, and NSMCE2 are direct RNF4 targets. We confirm PIAS1 as a key RNF4 substrate...
November 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/29166606/sumoylation-promotes-nuclear-import-and-stabilization-of-polo-like-kinase-1-to-support-its-mitotic-function
#7
Donghua Wen, Jianguo Wu, Lei Wang, Zheng Fu
As a pivotal mitotic regulator, polo-like kinase 1 (PLK1) is under highly coordinated and multi-layered regulation. However, the pathways that control PLK1's activity and function have just begun to be elucidated. PLK1 has recently been shown to be functionally modulated by post-translational modifications (PTMs), including phosphorylation and ubiquitination. Herein, we report that SUMOylation plays an essential role in regulating PLK1's mitotic function. We found that Ubc9 was recruited to PLK1 upon initial phosphorylation and activation by CDK1/cyclin B...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29151287/-desumoylation-of-protein-kinase-b1-inhibits-cell-proliferation-and-metastasis-of-hepatocellular-carcinoma
#8
F Sheng, Y M Shen, Q H Wan, Y X Li, X F Ma, Z M Jiang, D Y Zhang, X Z Liu, W H Wu
Objective: To investigate the effect of AKT1 deSUMOylation induced by Ubc9 silencing on the proliferation and metastasis of hepatocellular carcinoma (HCC) cells. Methods: The Ubc9 gene was silenced using RNA interference, and the expression levels of Ubc9, SUMO1 and AKT1 protein were detected by Western blot. Cell proliferation and cell cycle was analyzed by MTT and flow cytometry. Wound healing and transwell assays were used to detect the cell migration ability. Furthermore, the xenograft model was established, and tumor growth curves were drawn...
November 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/29138839/sumo1-ubc9%C3%A2-decreased-nox1-activity-inhibits-reactive-oxygen-species-generation-and-apoptosis-in-diabetic-retinopathy
#9
Jiaoli Hu, Pengcheng Xue, Xinbang Mao, Lin Xie, Guodong Li, Zhipeng You
Diabetic retinopathy (DR) is an increasing global health concern that causes vision loss and blindness. Reactive oxygen species (ROS) are considered to be a principal cause of DR. An important source of ROS is the oxidization of NADPH. In the present study, NADPH oxidase 1 (Nox1)‑expressing human retinal epithelial cell (HREC) lines were generated and infected with small ubiquitin‑like modifier 1 (SUMO1) and/or ubiquitin conjugating enzyme E2 I (UBC9) lentiviral pGMLV constructs. The viabilities, apoptotic capacities and ROS production levels of the HREC lines were quantified using Hoechst 33258, annexin V/propidium iodide and dichlorodihydrofluorescein diacetate assays, respectively...
November 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138491/sumoylation-regulates-the-localization-and-activity-of-polo-like-kinase-1-during-cell-cycle-in-the-silkworm-bombyx-mori
#10
Zhiqing Li, Qixin Cui, Jian Xu, Daojun Cheng, Xiaoyan Wang, Bingqian Li, Jae Man Lee, Qingyou Xia, Takahiro Kusakabe, Ping Zhao
Polo-like kinase 1 (Plk1) is a crucial cell cycle regulator by its specific localization and activity during cell cycle. It has been shown that the phosphorylation and ubiquitylation of Plk1 are required for its own activation and localization. Here, we report that SUMOylation regulates the activity of Plk1 in the lepidopteran insect of Bombyx mori. In the absence of SUMOylation, it causes the lost localization of Plk1 on centrosomes and kinetochores, as well as an uneven distribution in midzone. We further identify that the putative SUMOylation site of Bombyx Plk1 at lysine 466 is required for its localization on centrosomes, and K466 mutation in Plk1 could influence its interaction with Smt3/Ubc9 complex...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29127148/ubc9-binds-to-adap-and-is-required-for-rap1-membrane-recruitment-rac1-activation-and-integrin-mediated-t-cell-adhesion
#11
Yiwei Xiong, Chengjin Ye, Naiqi Yang, Madanqi Li, Hebin Liu
Although the immune adaptor adhesion and degranulation-promoting adaptor protein (ADAP) acts as a key mediator of integrin inside-out signaling leading to T cell adhesion, the regulation of this adaptor during integrin activation and clustering remains unclear. We now identify Ubc9, the sole small ubiquitin-related modifier E2 conjugase, as an essential regulator of ADAP where it is required for TCR-induced membrane recruitment of the small GTPase Rap1 and its effector protein RapL and for activation of the small GTPase Rac1 in T cell adhesion...
November 10, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29098326/sumoylation-controls-stem-cell-proliferation-and-regional-cell-death-through-hedgehog-signaling-in-planarians
#12
Manish Thiruvalluvan, Paul G Barghouth, Assaf Tsur, Limor Broday, Néstor J Oviedo
Mechanisms underlying anteroposterior body axis differences during adult tissue maintenance and regeneration are poorly understood. Here, we identify that post-translational modifications through the SUMO (Small Ubiquitin-like Modifier) machinery are evolutionarily conserved in the Lophotrocozoan Schmidtea mediterranea. Disruption of SUMOylation in adult animals by RNA-interference of the only SUMO E2 conjugating enzyme Ubc9 leads to a systemic increase in DNA damage and a remarkable regional defect characterized by increased cell death and loss of the posterior half of the body...
November 2, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/29069722/repression-of-sumoylation-pathway-by-grass-carp-reovirus-contributes-to-the-upregulation-of-pkr-in-an-ifn-independent-manner
#13
Fei Yu, Longlong Wang, Hao Wang, Jialu Sheng, Liqun Lu
SUMOylation, a post-translational modification, is involved in interaction between hosts and viruses, and participates in diverse cellular processes including inflammatory responses and innate immunity. Here, we investigated the interaction between reovirus infection and the cellular SUMOylation machinery using grass carp reovirus (GCRV) as a model. Full-length cDNAs of grass carp SUMO-1 and SUMO-2 were obtained and phylogenetic analysis indicated that they shared high homology with those of higher vertebrates...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29039464/hypothermia-inhibits-the-proliferation-of-bone-marrow-derived-mesenchymal-stem-cells-and-increases-tolerance-to-hypoxia-by-enhancing-sumoylation
#14
Xiaozhi Liu, Wenbo Ren, Zhongmin Jiang, Zhiguo Su, Xiaofang Ma, Yanxia Li, Rongcai Jiang, Jianning Zhang, Xinyu Yang
Hypothermia therapy has a positive effect on patients with severe brain injury. Recent studies have shown that mild hypothermia increases the survival of bone marrow-derived mesenchymal stem cells (BMSCs) in a hypoxic environment; however, the underlying mechanisms are not yet fully understood. Small ubiquitin-like modifiers (SUMOs) are sensitive to temperature stress reactions and are considered to exert a protective effect. In this study, we examined the protective effects of hypothermia on BMSCs in terms of SUMO protein modification...
September 29, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28966627/identification-of-optimal-reference-genes-for-expression-analysis-in-radish-raphanus-sativus-l-and-its-relatives-based-on-expression-stability
#15
Mengmeng Duan, Jinglei Wang, Xiaohui Zhang, Haohui Yang, Haiping Wang, Yang Qiu, Jiangping Song, Yangdong Guo, Xixiang Li
Radish (Raphanus sativus) is an important cruciferous root crop with a close relationship to Chinese cabbage (Brassica rapa). RT-qPCR is used extensively to evaluate the expression levels of target genes, and accurate measurement of target gene expression with this method is determined by the valid reference genes used for data nomalization in different experimental conditions. Screening for appropriate reference genes with stable expression based on RT-qPCR data is important for gene expression and functional analysis research in radish and its relatives...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28911869/degradation-of-nuclear-ubc9-induced-by-listeriolysin-o-is-dependent-on-k-efflux
#16
Jiexin Li, Wendy Wai-Ling Lam, Tsz-Wah Lai, Shannon Wing-Ngor Au
Listeriolysin O (LLO) is a pore-forming toxin produced by L. monocytogenes, and is belonged to a protein family of cholesterol-dependent cytolysins (CDCs). Previous studies have demonstrated that LLO triggers Ubc9 degradation and disrupts host SUMOylation to facilitate bacterial infection. However, the underlying mechanism of Ubc9 degradation is unclear. Here we show that LLO-induced down-regulation of Ubc9 is independent of Ubc9-SUMO interaction, however, it may involve phosphorylation signaling. Additionally, LLO exerts its effects primarily on nuclear Ubc9 and this process is mediated by K(+) efflux...
November 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28900032/nucleolar-and-spindle-associated-protein-1-nusap1-interacts-with-a-sumo-e3-ligase-complex-during-chromosome-segregation
#17
Christine A Mills, Aussie Suzuki, Anthony Arceci, Jin Yao Mo, Alex Duncan, Edward D Salmon, Michael J Emanuele
The mitotic spindle is composed of dynamic microtubules and associated proteins that together direct chromosome movement during mitosis. The spindle plays a vital role in accurate chromosome segregation fidelity and is a therapeutic target in cancer. Nevertheless, the molecular mechanisms by which many spindle-associated proteins function remains unknown. The nucleolar and spindle-associated protein NUSAP1 is a microtubule-binding protein implicated in spindle stability and chromosome segregation. We show here that NUSAP1 localizes to dynamic spindle microtubules in a unique chromosome-centric pattern, in the vicinity of overlapping microtubules, during metaphase and anaphase of mitosis...
October 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28888063/sumo-co-expression-modifies-kv-11-1-channel-activity
#18
Annette Buur Steffensen, Martin Nybo Andersen, Nancy Mutsaers, Amer Mujezinovic, Nicole Schmitt
AIM: The voltage-gated potassium channel KV 11.1 is the molecular basis for the IKr current which plays an important role in cardiac physiology. Its malfunction is associated with both inherited and acquired cardiac arrhythmias. Native currents differ from those in experimental models, suggesting additional regulatory mechanisms. We hypothesised that the post-translational modification sumoylation finetunes channel activity. METHODS: The functional effects of sumoylation on KV 11...
September 9, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28877029/impairments-in-age-dependent-ubiquitin-proteostasis-and-structural-integrity-of-selective-neurons-by-uncoupling-ran-gtpase-from-the-ran-binding-domain-3-of-ranbp2-and-identification-of-novel-mitochondrial-isoforms-of-ubiquitin-conjugating-enzyme-e2i-ubc9-and
#19
Hemangi Patil, Dosuk Yoon, Reshma Bhowmick, Yunfei Cai, Kyoung-In Cho, Paulo A Ferreira
The Ran-binding protein 2 (Ranbp2/Nup358) is a cytoplasmic and peripheral nucleoporin comprised of 4 Ran-GTP-binding domains (RBDs) that are interspersed among diverse structural domains with multifunctional activities. Our prior studies found that the RBD2 and RBD3 of Ranbp2 control mitochondrial motility independently of Ran-GTP-binding in cultured cells, whereas loss of Ran-GTP-binding to RBD2 and RBD3 are essential to support cone photoreceptor development and the survival of mature retinal pigment epithelium (RPE) in mice...
September 6, 2017: Small GTPases
https://www.readbyqxmd.com/read/28695742/human-regulatory-protein-ki-1-57-is-a-target-of-sumoylation-and-affects-pml-nuclear-body-formation
#20
Ângela Saito, Edmarcia E Souza, Fernanda C Costa, Gabriela V Meirelles, Kaliandra A Gonçalves, Marcos T Santos, Gustavo C Bressan, Mark E McComb, Catherine E Costello, Stephen A Whelan, Jörg Kobarg
Ki-1/57 is a nuclear and cytoplasmic regulatory protein first identified in malignant cells from Hodgkin's lymphoma. It is involved in gene expression regulation on both transcriptional and mRNA metabolism levels. Ki-1/57 belongs to the family of intrinsically unstructured proteins and undergoes phosphorylation by PKC and methylation by PRMT1. Previous characterization of its protein interaction profile by yeast two-hybrid screening showed that Ki-1/57 interacts with proteins of the SUMOylation machinery, the SUMO E2 conjugating enzyme UBC9 and the SUMO E3 ligase PIAS3, which suggested that Ki-1/57 could be involved with this process...
July 31, 2017: Journal of Proteome Research
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