Julio C Y Liu, Leena Ackermann, Saskia Hoffmann, Zita Gál, Ivo A Hendriks, Charu Jain, Louise Morlot, Michael H Tatham, Gian-Luca McLelland, Ronald T Hay, Michael Lund Nielsen, Thijn Brummelkamp, Peter Haahr, Niels Mailand
Protein SUMOylation provides a principal driving force for cellular stress responses, including DNA-protein crosslink (DPC) repair and arsenic-induced PML body degradation. In this study, using genome-scale screens, we identified the human E3 ligase TOPORS as a key effector of SUMO-dependent DPC resolution. We demonstrate that TOPORS promotes DPC repair by functioning as a SUMO-targeted ubiquitin ligase (STUbL), combining ubiquitin ligase activity through its RING domain with poly-SUMO binding via SUMO-interacting motifs, analogous to the STUbL RNF4...
April 22, 2024: Nature Structural & Molecular Biology