keyword
MENU ▼
Read by QxMD icon Read
search

Adipocyte ipsc

keyword
https://www.readbyqxmd.com/read/27904680/enhancement-of-early-cardiac-differentiation-of-dedifferentiated-fat-cells-by-dimethyloxalylglycine-via-notch-signaling-pathway
#1
Fuhai Li, Zongzhuang Li, Zhi Jiang, Ye Tian, Zhi Wang, Wei Yi, Chenyun Zhang
Background: Hypoxia has been reported to possess the ability to induce mature lipid-filled adipocytes to differentiate into fibroblast-like multipotent dedifferentiated fat (DFAT) cells and stem cells such as iPSCs (interstitial pluripotent stem cells) and ESCs (embryonic stem cells) and then to differentiate into cardiomyocytes. However, the effect of hypoxia on cardiac differentiation of DFAT cells and its underlying molecular mechanism remains to be investigated. Objective: To investigate the role of hypoxia in early cardiac differentiation of DFAT cells and the underlying molecular mechanism...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27864696/generation-and-differentiation-of-induced-pluripotent-stem-cells-reveal-ankylosing-spondylitis-risk-gene-expression-in-bone-progenitors
#2
Gerlinde Layh-Schmitt, Shajia Lu, Fatemeh Navid, Stephen R Brooks, Emily Lazowick, Kathryn M Davis, Cristina Montagna, Massimo Gadina, Robert A Colbert
Axial spondyloarthritis (axSpA), which encompasses ankylosing spondylitis, is a complex genetic disease. Aberrant bone formation is a key feature of pathogenesis that can lead to ankylosis of the spine. Our objective is to determine, whether genes whose variants confer susceptibility to AS are expressed in bone progenitors like mesenchymal stem cells (MSCs). Since MSCs from bone marrow is difficult to obtain, we first examined, whether MSCs can be derived from induced pluripotent stem cells (iPSCs). Dermal fibroblasts of two axSpA patients and one healthy control were reprogrammed into iPSCs using a Sendai virus vector encoding pluripotency genes...
November 18, 2016: Clinical Rheumatology
https://www.readbyqxmd.com/read/27240827/multipotent-differentiation-of-human-dental-pulp-stem-cells-a-literature-review
#3
N Nuti, C Corallo, B M F Chan, M Ferrari, B Gerami-Naini
The advent of regenerative medicine has brought us the opportunity to regenerate, modify and restore human organs function. Stem cells, a key resource in regenerative medicine, are defined as clonogenic, self-renewing, progenitor cells that can generate into one or more specialized cell types. Stem cells have been classified into three main groups: embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult/postnatal stem cells (ASCs). The present review focused the attention on ASCs, which have been identified in many perioral tissues such as dental pulp, periodontal ligament, follicle, gingival, alveolar bone and papilla...
May 30, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27206577/generation-of-neural-crest-like-cells-from-human-periodontal-ligament-cell-derived-induced-pluripotent-stem-cells
#4
Atsushi Tomokiyo, Kim Hynes, Jia Ng, Danijela Menicanin, Esther Camp, Agnes Arthur, Stan Gronthos, P Mark Bartold
Neural crest cells (NCC) hold great promise for tissue engineering, however the inability to easily obtain large numbers of NCC is a major factor limiting their use in studies of regenerative medicine. Induced pluripotent stem cells (iPSC) are emerging as a novel candidate that could provide an unlimited source of NCC. In the present study, we examined the potential of neural crest tissue-derived periodontal ligament (PDL) iPSC to differentiate into neural crest-like cells (NCLC) relative to iPSC generated from a non-neural crest derived tissue, foreskin fibroblasts (FF)...
May 21, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27130501/a-bioluminescent-assay-for-measuring-glucose-uptake
#5
Michael P Valley, Natasha Karassina, Natsuyo Aoyama, Coby Carlson, James J Cali, Jolanta Vidugiriene
Identifying activators and inhibitors of glucose uptake is critical for both diabetes management and anticancer therapy. To facilitate such studies, easy-to-use nonradioactive assays are desired. Here we describe a bioluminescent glucose uptake assay for measuring glucose transport in cells. The assay is based on the uptake of 2-deoxyglucose and the enzymatic detection of the 2-deoxyglucose-6-phosphate that accumulates. Uptake can be measured from a variety of cell types, it can be inhibited by known glucose transporter inhibitors, and the bioluminescent assay yields similar results when compared with the radioactive method...
July 15, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27108524/establishment-and-adipocyte-differentiation-of-polycystic-ovary-syndrome-derived-induced-pluripotent-stem-cells
#6
Sheng Yang, Shufang Ding, Xianglong Jiang, Bolan Sun, Qianhua Xu
OBJECTIVE: To establish a new biological cell model and approach to mimic abnormal lipid metabolism of polycystic ovary syndrome (PCOS) in vitro. MATERIALS AND METHODS: Epithelial cells from PCOS patients were reprogrammed to pluripotency by retroviral transduction using defined factors. Morphology, growth characteristics, karyotype, gene expression and differentiation in vitro and in vivo were detected by identification protocol of human embryonic stem cells (ESCs)...
June 2016: Cell Proliferation
https://www.readbyqxmd.com/read/26981171/human-pluripotent-stem-cells-towards-therapeutic-development-for-the-treatment-of-lifestyle-diseases
#7
REVIEW
Miwako Nishio, Masako Nakahara, Akira Yuo, Kumiko Saeki
There are two types of human pluripotent stem cells: Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), both of which launched themselves on clinical trials after having taken measures to overcome problems: Blocking rejections by immunosuppressants regarding ESCs and minimizing the risk of tumorigenicity by depleting exogenous gene components regarding iPSCs. It is generally assumed that clinical applications of human pluripotent stem cells should be limited to those cases where there are no alternative measures for treatments because of the risk in transplanting those cells to living bodies...
February 26, 2016: World Journal of Stem Cells
https://www.readbyqxmd.com/read/26884346/muse-cells-provide-the-pluripotency-of-mesenchymal-stem-cells-direct-contribution-of-muse-cells-to-tissue-regeneration
#8
Mari Dezawa
While mesenchymal stem cells (MSCs) are easily accessible from mesenchymal tissues, such as bone marrow and adipose tissue, they are heterogeneous, and their entire composition is not fully identified. MSCs are not only able to differentiate into osteocytes, chondrocytes, and adipocytes, which belong to the same mesodermal lineage, but they are also able to cross boundaries between mesodermal, ectodermal, and endodermal lineages, and differentiate into neuronal- and hepatocyte-like cells. However, the ratio of such differentiation is not very high, suggesting that only a subpopulation of the MSCs participates in this cross-lineage differentiation phenomenon...
2016: Cell Transplantation
https://www.readbyqxmd.com/read/26365511/reprogrammed-functional-brown-adipocytes-ameliorate-insulin-resistance-and-dyslipidemia-in-diet-induced-obesity-and-type-2-diabetes
#9
Tsunao Kishida, Akika Ejima, Kenta Yamamoto, Seiji Tanaka, Toshiro Yamamoto, Osam Mazda
Brown adipocytes (BAs) play important roles in body temperature regulation, energy balance, and carbohydrate and lipid metabolism. Activities of BAs are remarkably diminished in obese and diabetic patients, providing possibilities of transplanting functional BAs resulting in therapeutic benefit. Here, we show generation of functional BAs by cellular reprogramming procedures. Transduction of the PRDM16 gene into iPSC-derived embryoid bodies induced BA phenotypes (iBAs). Moreover, normal human fibroblasts were directly converted into BAs (dBAs) by C/EBP-β and C-MYC gene transduction...
October 13, 2015: Stem Cell Reports
https://www.readbyqxmd.com/read/26282538/mesenchymal-stem-cells-derived-from-human-induced-pluripotent-stem-cells-retain-adequate-osteogenicity-and-chondrogenicity-but-less-adipogenicity
#10
Ran Kang, Yan Zhou, Shuang Tan, Guangqian Zhou, Lars Aagaard, Lin Xie, Cody Bünger, Lars Bolund, Yonglun Luo
INTRODUCTION: Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective of this study is to systematically characterize the mesenchymal characteristics of the iPSC-MSCs by comparing them to bone marrow-derived MSCs (BM-MSCs). METHODS: Two iPSC-MSC lines (named as mRNA-iPSC-MSC-YL001 and lenti-iPSC-MSC-A001) and one BM-MSC line were used for the study...
2015: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/25797907/human-mesenchymal-stem-cells-current-trends-and-future-prospective
#11
REVIEW
Imran Ullah, Raghavendra Baregundi Subbarao, Gyu Jin Rho
Stem cells are cells specialized cell, capable of renewing themselves through cell division and can differentiate into multi-lineage cells. These cells are categorized as embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) and adult stem cells. Mesenchymal stem cells (MSCs) are adult stem cells which can be isolated from human and animal sources. Human MSCs (hMSCs) are the non-haematopoietic, multipotent stem cells with the capacity to differentiate into mesodermal lineage such as osteocytes, adipocytes and chondrocytes as well ectodermal (neurocytes) and endodermal lineages (hepatocytes)...
2015: Bioscience Reports
https://www.readbyqxmd.com/read/25404570/deciphering-the-heterogeneity-in-dna-methylation-patterns-during-stem-cell-differentiation-and-reprogramming
#12
Xiaojian Shao, Cuiyun Zhang, Ming-An Sun, Xuemei Lu, Hehuang Xie
BACKGROUND: Human induced pluripotent stem cells (iPSCs) have a wide range of applications throughout the fields of basic research, disease modeling and drug screening. Epigenetic instable iPSCs with aberrant DNA methylation may divide and differentiate into cancer cells. Unfortunately, little effort has been taken to compare the epigenetic variation in iPSCs with that in differentiated cells. Here, we developed an analytical procedure to decipher the DNA methylation heterogeneity of mixed cells and further exploited it to quantitatively assess the DNA methylation variation in the methylomes of adipose-derived stem cells (ADS), mature adipocytes differentiated from ADS cells (ADS-adipose) and iPSCs reprogrammed from ADS cells (ADS-iPSCs)...
2014: BMC Genomics
https://www.readbyqxmd.com/read/25111735/generation-of-rat-induced-pluripotent-stem-cells-from-a-new-model-of-metabolic-syndrome
#13
Nana Takenaka-Ninagawa, Yuka Kawabata, Shogo Watanabe, Kohzo Nagata, Shigeko Torihashi
We recently characterized DahlS.Z-Leprfa/Leprfa (DS/obese) rats, derived from a cross between Dahl salt-sensitive rats and Zucker rats, as a new animal model of metabolic syndrome (MetS). Although the phenotype of DS/obese rats is similar to that of humans with MetS, the pathophysiological and metabolic characteristics in each cell type remain to be clarified. Hence, the establishment of induced pluripotent stem cells (iPSCs) derived from MetS rats is essential for investigations of MetS in vitro. Reports of rat iPSCs (riPSCs), however, are few because of the difficulty of comparing to other rodents such as mouse...
2014: PloS One
https://www.readbyqxmd.com/read/25038011/engineering-patient-specific-valves-using-stem-cells-generated-from-skin-biopsy-specimens
#14
David L Simpson, Brody Wehman, Yekaterina Galat, Sudhish Sharma, Rachana Mishra, Vasiliy Galat, Sunjay Kaushal
BACKGROUND: Pediatric patients requiring valve replacement will likely require reoperations due to a progressive deterioration of valve durability and limited repair and growth potential. To address these concerns, we sought to generate a biologically active pulmonary valve using patient-specific valvular cells and decellularized human pulmonary valves. METHODS: We generated induced pluripotent stem cells (iPSCs) by reprogramming skin fibroblast cells. We then differentiated iPSCs to mesenchymal stem cells (iPCSs-MSCs) using culture conditions that favored an epithelial-to-mesenchymal transition...
September 2014: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/24795699/mesenchymal-stem-cells-new-players-in-retinopathy-therapy
#15
REVIEW
Gangaraju Rajashekhar
Retinopathies in human and animal models have shown to occur through loss of pericytes resulting in edema formation, excessive immature retinal angiogenesis, and neuronal apoptosis eventually leading to blindness. In recent years, the concept of regenerating terminally differentiated organs with a cell-based therapy has evolved. The cells used in these approaches are diverse and include tissue-specific endogenous stem cells, endothelial progenitor (EPC), embryonic stem cells, induced pluripotent stem cells (iPSC) and mesenchymal stem cells (MSC)...
2014: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/24567789/current-applications-of-adipose-derived-stem-cells-and-their-future-perspectives
#16
REVIEW
Eun-Hee Kim, Chan Yeong Heo
Adult stem cells have a great potential to treat various diseases. For these cell-based therapies, adipose-derived stem cells (ADSCs) are one of the most promising stem cell types, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). ESCs and iPSCs have taken center stage due to their pluripotency. However, ESCs and iPSCs have limitations in ethical issues and in identification of characteristics, respectively. Unlike ESCs and iPSCs, ADSCs do not have such limitations and are not only easily obtained but also uniquely expandable...
January 26, 2014: World Journal of Stem Cells
https://www.readbyqxmd.com/read/24367908/generation-of-functional-mesenchymal-stem-cells-from-different-induced-pluripotent-stem-cell-lines
#17
Kim Hynes, Danijela Menicanin, Krzysztof Mrozik, Stan Gronthos, P Mark Bartold
The therapeutic potential of mesenchymal stem cells (MSC) has highlighted the need for identifying easily accessible and reliable sources of these cells. An alternative source for obtaining large populations of MSC is through the controlled differentiation of induced pluripotent stem cells (iPSC). In the present study, colonies of iPSC were cultured in MSC culture media for 2 weeks. Serial passaging then selected for fast growing MSC-like cells with a typical fibroblastic morphology and the capacity to proliferate on standard culture flasks without feeder cells...
May 15, 2014: Stem Cells and Development
https://www.readbyqxmd.com/read/24279868/human-induced-pluripotent-stem-cell-derived-mesenchymal-stem-cell-seeding-on-calcium-phosphate-scaffold-for-bone-regeneration
#18
Minghui Tang, Wenchuan Chen, Jun Liu, Michael D Weir, Linzhao Cheng, Hockin H K Xu
Tissue engineering provides an important approach for bone regeneration. Calcium phosphate cement (CPC) can be injected to fill complex-shaped bone defects with excellent osteoconductivity. Induced pluripotent stem cells (iPSCs) are exciting for regenerative medicine due to their potential to proliferate and differentiate into cells of all three germ layers. To date, there has been no report on iPSC seeding with CPC scaffolds. The objectives of this study were to (1) obtain iPSC-derived mesenchymal stem cells (iPSC-MSCs); (2) seed iPSC-MSCs on CPC scaffold for the first time to investigate cell attachment and proliferation; and (3) investigate osteogenic differentiation of iPSC-MSCs on CPC and mineral synthesis by the cells...
April 2014: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/24182316/direct-conversion-of-adipocyte-progenitors-into-functional-neurons
#19
Yuanyuan Yang, Jiao Jiao, Rui Gao, Hui Yao, Xiao-Fang Sun, Shaorong Gao
Ectopic expression of a set of transcription factors in somatic cells could reprogram the differentiated cell fate into the pluripotent state, and the resultant so-called induced pluripotent stem cells (iPSCs) have been proposed as seed cells for cell therapy-based regenerative medicine. However, their tumorigenicity limited the further application of iPSCs clinically. More recently, collected evidence has shown that differentiated somatic cells could be directly converted into other types of somatic cells through overexpression of transcription factors enriched in the targeted cell types...
December 2013: Cellular Reprogramming
https://www.readbyqxmd.com/read/23763475/oxidative-stress-inhibits-adhesion-and-transendothelial-migration-and-induces-apoptosis-and-senescence-of-induced-pluripotent-stem-cells
#20
Yi Wu, Xueqing Zhang, Xueling Kang, Ning Li, Rong Wang, Tiantian Hu, Meng Xiang, Xinhong Wang, Wenjun Yuan, Alex Chen, Dan Meng, Sifeng Chen
Oxidative stress caused by cellular accumulation of reactive oxygen species (ROS) is a major contributor to disease and cell death. However, how induced pluripotent stem cells (iPSC) respond to different levels of oxidative stress is largely unknown. Here, we investigated the effect of H2 O2 -induced oxidative stress on iPSC function in vitro. Mouse iPSC were treated with H2 O2 (25-100 μmol/L). IPSC adhesion, migration, viability, apoptosis and senescence were analysed. Expression of adhesion-related genes, stress defence genes, and osteoblast- and adipocyte-associated genes were determined by reverse transcription polymerase chain reaction...
September 2013: Clinical and Experimental Pharmacology & Physiology
keyword
keyword
37828
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"