Dania Altulea, Joost C van den Born, Arjan Diepstra, Laura Bungener, Dagmar Terpstra, Bouke G Hepkema, Rosa Lammerts, Peter Heeringa, Sebastiaan Heidt, Henny Otten, Leon Reteig, Gonca E Karahan, Stefan P Berger, Jan-Stephan Sanders
BACKGROUND: Repeated exposure to sensitizing events can activate HLA-specific memory B cells, leading to the production of donor-specific memory B cell antibodies (DSAm) that pose a risk for antibody-mediated rejection (ABMR) in kidney transplant recipients (KTRs). This single-center retrospective study aimed to identify DSAm and assess their association with outcomes in a cohort of KTRs with pretransplant serum donor-specific antibodies (DSA). METHODS: We polyclonally activated pretransplant peripheral blood mononuclear cells (PBMCs) from 60 KTRs in vitro, isolated and quantified IgG from the culture supernatant using ELISA, and analyzed the HLA antibodies of eluates with single antigen bead (SAB) assays, comparing them to the donor HLA typing for potential DSAm...
2024: Frontiers in Immunology