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https://www.readbyqxmd.com/read/29779271/-local-injection-of-exogenous-nerve-growth-factor-improves-early-bone-maturation-of-implants
#1
Yang Yao, Yu Du, Xia Gu, Meng-Kai Guang, Bo Huang, Ping Gong
OBJECTIVE: To investigate the effects of nerve growth factor (NGF) in the osteogenic action of implants and the maturation and reconstruction changes in bone tissues in the early stage of osseointegration. METHODS: The mouse implant model was established by placing titanium in the femoral head of the mouse and locally injecting NGF in the implant zone. On 1, 2 and 4 weeks after operation, stain samples were collected from animals using hematoxylin-eosin (HE) staining and Masson staining...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779270/-protective-effects-of-hydrogen-rich-medium-on-lipopolysaccharides-induced-injury-in-human-periodontal-ligament-cells
#2
Min Zhou, Zuo-Lin Wang
OBJECTIVE: In this study, lipopolysaccharides (LPS) was used to damage human periodontal ligament cells (hPDLCs) and consequently investigate the protective effects of hydrogen on reducing oxidative stress and cell apoptosis rate. METHODS: hPDLCs were isolated, and then cultured with normal medium+1 μg·mL⁻¹ LPS or with hydrogen-rich medium+
1 μg·mL⁻¹ LPS. Cell proliferation activity was assessed using a cell counting kit-8 (CCK-8), and lactic dehydrogenase (LDH) release was also detected...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779121/a-comprehensive-comparable-study-of-the-physiological-properties-of-four-microalgal-species-under-different-light-wavelength-conditions
#3
Yu Zhong, Peng Jin, Jay J Cheng
Microalgae treated with blue light have potential for production of human nutrition supplement and biofuel due to their higher biomass productivity and favorable fatty acid composition. Chlorella vulgaris, Chlorella pyrenoidosa, Scenedesmus quadricauda and Scenedesmus obliquus are representative green microalgae which are widely reported for algal production. In this study, we provide a systematic investigation of the biomass productivity, photosynthetic pigments, chlorophyll fluorescence and fatty acid content of the four green microalgae...
May 19, 2018: Planta
https://www.readbyqxmd.com/read/29779040/association-between-unexplained-thickened-nuchal-translucency-and-adverse-pregnancy-outcomes
#4
Santee Tiyatha, Sirinart Sirilert, Ratanaporn Sekararithi, Theera Tongsong
OBJECTIVE: To determine the association between unexplained increased nuchal translucency (INT) and adverse pregnancy outcomes. PATIENTS AND METHODS: The prospective database of our fetal down screening project was accessed to retrieve the records with NT measurement and complete follow-up. Pregnancies with pre-existing medical diseases, fetal chromosomal or structural abnormalities were excluded. The selected pregnancies were classified into the INT groups (> 95th percentile), the normal (< 95th percentile) group...
May 19, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29779024/a-randomized-clinical-trial-of-metformin-to-treat-autosomal-dominant-polycystic-kidney-disease
#5
Stephen L Seliger, Kaleab Z Abebe, Kenneth R Hallows, Dana C Miskulin, Ronald D Perrone, Terry Watnick, Kyongtae Tae Bae
BACKGROUND: Metformin inhibits cyclic AMP generation and activates AMP-activated protein kinase (AMPK), which inhibits the cystic fibrosis transmembrane conductance regulator and Mammalian Target of Rapamycin pathways. Together these effects may reduce cyst growth in autosomal dominant polycystic kidney disease (ADPKD). METHODS: A phase II, double-blinded randomized placebo-controlled trial of 26 months duration. Participants will include nondiabetic adults (n = 96) aged 18-60 years, with an estimated glomerular filtration rate (eGFR) ≥50 mL/min/1...
May 18, 2018: American Journal of Nephrology
https://www.readbyqxmd.com/read/29779023/parathyroid-hormone-fibroblast-growth-factor-23-and-parameters-of-phosphate-reabsorption
#6
Kenneth R Phelps, Darius L Mason
BACKGROUND: The serum phosphorus concentration ([P]s) is the sum of EP/Ccr and TRP/Ccr, where Ccr is creatinine clearance and EP and TRP are rates of excretion and reabsorption of phosphate. In chronic kidney disease (CKD), parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) mediate reduction of TRP/Ccr, and [PTH] and [FGF23] are linear functions of EP/Ccr. If controls and patients with CKD are considered together, TRP/Ccr is a hyperbolic function of EP/Ccr. Given these observations, we hypothesized that hyperbolas would describe relationships of phosphate reabsorption to [PTH] and [FGF23]...
May 18, 2018: American Journal of Nephrology
https://www.readbyqxmd.com/read/29779013/treatment-of-sec62-over-expressing-tumors-by-thapsigargin-and-trifluoperazine
#7
Christina Körbel, Maximilian Linxweiler, Florian Bochen, Silke Wemmert, Bernhard Schick, Markus Meyer, Hans Maurer, Michael D Menger, Richard Zimmermann, Markus Greiner
Treatment with analogues of the SERCA-inhibitor Thapsigargin is a promising new approach for a wide variety of cancer entities. However, our previous studies on various tumor cells suggested resistance of SEC62 over-expressing tumors to this treatment. Therefore, we proposed the novel concept that e.g. lung-, prostate-, and thyroid-cancer patients should be tested for SEC62 over-expression, and developed a novel therapeutic strategy for a combinatorial treatment of SEC62 over-expressing tumors. The latter was based on the observations that treatment of SEC62 over-expressing tumor cells with SEC62-targeting siRNAs showed less resistance to Thapsigargin as well as a reduction in migratory potential and that the siRNA effects can be mimicked by the Calmodulin antagonist Trifluoperazine...
May 19, 2018: Biomolecular Concepts
https://www.readbyqxmd.com/read/29778955/controlled-release-urea-reduced-nitrogen-leaching-and-improved-nitrogen-use-efficiency-and-yield-of-direct-seeded-rice
#8
Shugang Zhang, Tianlin Shen, Yuechao Yang, Yuncong C Li, Yongshan Wan, Min Zhang, Yafu Tang, Samuel C Allen
The use of controlled-release urea (CRU) has become one of best management practices for increasing crop yield and improving nitrogen (N) use efficiency (NUE). However, the effects of CRU on direct-seeded rice are not well understood while direct-seeding has gradually replaced transplanting due to increasing labor cost and lack of irrigation water. The objective of this two-year field experiment was to compare the effects of the CRU at four rates (120, 180, 240 and 360 kg N ha-1 , CRU1, CRU2, CRU3 and CRU4, respectively) with a conventional urea fertilizer (360 kg N ha-1 ; U) and a control (no N fertilizer applied; CK) on yield, biomass, NUE of direct-seeded rice and soil nutrients...
May 17, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29778938/effects-of-short-and-long-term-exposure-of-silver-nanoparticles-and-silver-ions-to-nitrosomonas-europaea-biofilms-and-planktonic-cells
#9
L K Barker, J R Giska, T S Radniecki, L Semprini
The increasing use of silver nanoparticles (AgNPs) in consumer products, and their resulting influx into wastewater, may pose a threat to biological nutrient removal in wastewater treatment plants. Planktonic ammonia-oxidizing bacteria (AOB), which convert ammonia to nitrite in the first step of nitrification, are highly sensitive to AgNPs and their released silver ions (Ag+ ), but the sensitivity of AOB biofilms to AgNPs and Ag+ is less clear. This study demonstrated that biofilms of Nitrosomonas europaea, a model AOB, were more resistant to both short-term and long-term exposure to AgNP and Ag+ than planktonic cells...
May 4, 2018: Chemosphere
https://www.readbyqxmd.com/read/29778896/formation-and-transformation-of-calcium-phosphate-phases-under-biologically-relevant-conditions-experiments-and-modelling
#10
Agnese Carino, Christian Ludwig, Antonio Cervellino, Elisabeth Müller, Andrea Testino
The experimental data on calcium phosphates formation were collected in dilute solution at constant pH (7.40) and temperature (37.0 °C) at different levels of ionic strength (IS). The evolution of the solid phase formation is described in detail using a thermodynamic-kinetic model. The thermodynamic model takes into account all relevant chemical species as well as Posner's clusters; the kinetic model, based on the discretized population balance approach, accounts for the solid formation from solution. The experimental data are consistent with an initial formation of dicalcium phosphate dihydrate (DCPD, brushite), which dominates the nucleation rate, and its rapid transformation into octacalcium phosphate (OCP) or hydroxyapatite (HA), which dominates the growth rate...
May 17, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29778800/phenotypic-and-genotypic-diversity-of-lactobacillus-buchneri-strains-isolated-from-spoiled-fermented-cucumber
#11
Katheryne V Daughtry, Suzanne D Johanningsmeier, Rosemary Sanozky-Dawes, Todd R Klaenhammer, Rodolphe Barrangou
Lactobacillus buchneri is a Gram-positive, obligate heterofermentative, facultative anaerobe commonly affiliated with spoilage of food products. Notably, L. buchneri is able to metabolize lactic acid into acetic acid and 1,2-propanediol. Although beneficial to the silage industry, this metabolic capability is detrimental to preservation of cucumbers by fermentation. The objective of this study was to characterize isolates of L. buchneri purified from both industrial and experimental fermented cucumber after the onset of secondary fermentation...
May 9, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29778787/efficacy-of-palbociclib-combinations-in-hormone-receptor-positive-metastatic-breast-cancer-patients-after-prior-everolimus-treatment
#12
Ajay Dhakal, Christina M Matthews, Ellis Glenn Levine, Kilian Elizabeth Salerno, Fan Zhang, Kazuaki Takabe, Amy P Early, Stephen B Edge, Tracy O'Connor, Thaer Khoury, Jessica S Young, Mateusz Opyrchal
PURPOSE: Outcome data on hormone receptor positive (HR+ ), human epidermal growth factor receptor 2 (HER2) nonamplified (HER2- ) metastatic breast cancer (MBC) treated with palbociclib after treatment with everolimus are lacking. The PALOMA-3 trial, showing benefit of palbociclib plus fulvestrant compared to fulvestrant alone in HR+ HER2- MBC after progression while receiving endocrine therapy excluded women previously treated with everolimus. The objective of this study was to examine outcomes of HR+ HER2- MBC with prior exposure to everolimus while receiving palbociclib-based therapy...
April 28, 2018: Clinical Breast Cancer
https://www.readbyqxmd.com/read/29778677/growth-impacts-of-saharan-dust-mineral-nutrients-and-co-2-on-a-planktonic-herbivore-in-southern-mediterranean-lakes
#13
Manuel Villar-Argaiz, Marco J Cabrerizo, Juan Manuel González-Olalla, Macarena S Valiñas, Sanja Rajic, Presentación Carrillo
Rising levels of CO2 can boost plant biomass but reduce its quality as a food source for herbivores. However, significant uncertainties remain as to the degree to which the effect is modulated by other environmental factors and the underlying processes causing these responses in nature. To address these questions, we carried out CO2 -manipulation experiments using natural seston from three lakes under nutrient-enriched conditions (mimicking eutrophication and atmospheric dust-input processes) as a food source for the planktonic Daphnia pulicaria...
May 17, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29778472/effects-of-ad-libitum-milk-replacer-feeding-and-butyrate-supplementation-on-behavior-immune-status-and-health-of-holstein-calves-in-the-postnatal-period
#14
C Gerbert, D Frieten, C Koch, G Dusel, K Eder, T Stefaniak, J Bajzert, P Jawor, A Tuchscherer, H M Hammon
Animal welfare in dairy calf husbandry depends on calf rearing and is probably improved by intensive milk feeding programs. In addition, butyrate supplementation in milk replacer (MR) stimulates postnatal growth and may affect the immune system in calves. We have investigated the combined effects of ad libitum MR feeding and butyrate supplementation on feeding behavior, health, and the immune responses in calves. Holstein calves (n = 64) were examined from birth until wk 11 of age. Calves received MR either ad libitum (Adl) or restrictively (Res) with (AdlB+, ResB+) or without (AdlB-, ResB-) 0...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29778444/artificial-structures-alter-kelp-functioning-across-an-urbanised-estuary
#15
M Mayer-Pinto, K A Dafforn, A B Bugnot, T M Glasby, E L Johnston
Assessments of human impacts on natural habitats often focus on the abundance of component species, yet physiological and/or sub-lethal effects of stressors on functional attributes may be equally important to consider. Here we evaluated how artificial structures, an integral part of urbanisation in the marine environment, affects key functional properties of the habitat-forming kelp Ecklonia radiata. Given that stressors rarely occur in isolation, we assessed the effects of infrastructure across an urbanised estuary...
May 5, 2018: Marine Environmental Research
https://www.readbyqxmd.com/read/29778422/sp1-induced-upregulation-of-lncrna-pandar-predicts-adverse-phenotypes-in-retinoblastoma-and-regulates-cell-growth-and-apoptosis-in-vitro-and-in-vivo
#16
Lijie Sheng, Jing Wu, Xuewu Gong, Duo Dong, Xiaoxue Sun
Retinoblastoma (RB) is an intraocular malignancy for children and has a high mortality rate. Long non-coding RNAs (lncRNAs) are emerging as gene regulators and biomarkers in various malignancies. PANDAR is a novel cancer-related lncRNA that dysregulated in several types of cancers. However, its clinical value and potential effects on RB remains unclear. RT-qPCR was used to assess the relative expression of PANDAR in RB tissues and cells. Additionally, chromatin immunoprecipitation (ChIP) and luciferase reporter assays were performed to investigate whether SP1 could bind to the promoter region of PANDAR and activate its transcription...
May 17, 2018: Gene
https://www.readbyqxmd.com/read/29778143/efficiency-in-hydrocarbon-degradation-and-biosurfactant-production-by-joostella-sp-a8-when-grown-in-pure-culture-and-consortia
#17
Carmen Rizzo, Alessandro Ciro Rappazzo, Luigi Michaud, Emilio De Domenico, Carlos Rochera, Antonio Camacho, Angelina Lo Giudice
Joostella strains are emerging candidates for biosurfactant production. Here such ability was analyzed for Joostella strain A8 in comparison with Alcanivorax strain A53 and Pseudomonas strain A6, all previously isolated from hydrocarbon enrichment cultures made of polychaete homogenates. In pure cultures Joostella sp. A8 showed the highest stable emulsion percentage (78.33%), hydrophobicity rate (62.67%), and an optimal surface tension reduction during growth in mineral medium supplemented with diesel oil (reduction of about 12mN/m), thus proving to be highly competitive with Alcanivorax and Pseudomonas strains...
May 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29778102/effects-of-extending-the-length-of-pro-oestrus-in-an-oestradiol-and-progesterone-based-oestrus-synchronisation-program-on-ovarian-function-uterine-environment-and-pregnancy-establishment-in-beef-heifers
#18
J J de la Mata, R Núñez-Olivera, F Cuadro, D Bosolasco, V de Brun, A Meikle, G A Bó, A Menchaca
The aim of the present study was to investigate the effects of a strategy for extending pro-oestrus (the interval between luteolysis and ovulation) in an oestrus synchronisation protocol (named J-Synch) in beef heifers on follicular growth, sexual steroid concentrations, the oestrogen receptor ERα and progesterone receptors (PR) in the uterus, insulin-like growth factor (IGF) 1 and pregnancy rates. In Experiment 1, heifers treated with the new J-Synch protocol had a longer pro-oestrus period than those treated with the conventional protocol (mean (±s...
May 21, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29778059/tracking-pollutants-in-dietary-fish-oil-from-ocean-to-table
#19
Sheng-Xiang Sun, Xue-Ming Hua, Yun-Yun Deng, Yun-Ni Zhang, Jia-Min Li, Zhao Wu, Samwel Mchele Limbu, Da-Sheng Lu, Hao-Wen Yin, Guo-Quan Wang, Rune Waagbø, Frøyland Livar, Mei-Ling Zhang, Zhen-Yu Du
Dietary fish oil used in aquafeed transfers marine pollutants to farmed fish. However, the entire transfer route of marine pollutants in dietary fish oil from ocean to table fish has not been tracked quantitatively. To track the entire transfer route of marine pollutants from wild fish to farmed fish through dietary fish oil and evaluate the related human health risks, we obtained crude and refined fish oils originating from the same batch of wild ocean anchovy and prepared fish oil-containing purified aquafeeds to feed omnivorous lean Nile tilapia and carnivorous fatty yellow catfish for eight weeks...
May 16, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29778049/toxicity-screening-of-biochar-mineral-composites-using-germination-tests
#20
Jan Mumme, Josephine Getz, Munoo Prasad, Ulf Lüder, Jürgen Kern, Ondřej Mašek, Wolfram Buss
This study assessed the properties and toxicity (water cress germination trials) of 38 waste-derived, novel biochar-mineral composites (BMCs) produced via slow pyrolysis and hydrothermal carbonization (hydrochars). The biochars were produced from sewage sludge and compost-like output (CLO) by varying the type of mineral additive (zeolite, wood ash and lignite fly ash), the mineral-to-feedstock ratio and the carbonization process. While pure hydrochars completely inhibited germination of water cress, this effect was ameliorated by mineral additives...
May 8, 2018: Chemosphere
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