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https://www.readbyqxmd.com/read/29455876/laser-ablation-facilitates-closure-of-chronic-enterocutaneous-fistulae
#1
Ravi N Srinivasa, Rajiv N Srinivasa, Joseph J Gemmete, Anthony N Hage, William Sherk, Jeffrey Forris Beecham Chick
This report describes the use of laser ablation for treatment of chronic enterocutaneous fistulae (ECFs) after failure of conservative therapy. Three patients underwent laser ablation for treatment of 8 ECFs. Mean duration of fistula patency was 28 months with mean fistula output of 134 mL/day. The initial technical success was 100% with no major or minor complications. Three ECFs required repeat treatment. At mean follow-up of 53 days, 7 of the fistulae were occluded. One fistula showed a markedly reduced output of 10 mL/day...
March 2018: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/29448989/redefining-bowel-regimen-pharmacologic-strategies-and-nutritional-considerations-in-the-management-of-small-bowel-fistulas
#2
REVIEW
Sara E Parli, Carolyn Pfeifer, Douglas R Oyler, Barbara Magnuson, Levi D Procter
Enterocutaneous fistulae (ECF) and enteroatmospheric fistulae (EAF) are difficult complications that primarily arise after abdominal surgical procedures. Development of an ECF or EAF carries significant mortality and morbidity. Effective management of patients with these disease states requires a multidisciplinary approach, which includes surgical, pharmacotherapeutic, and nutritional interventions. This review focuses on the medical and nutritional management of ECF/EAF, providing background on drug agents and nutritional strategies that may be helpful in reducing effluent volume, optimizing fistula healing, and maintaining nutritional health...
February 12, 2018: American Journal of Surgery
https://www.readbyqxmd.com/read/29438446/a-thioredoxin-dependent-peroxiredoxin-q-from-corynebacterium-glutamicum-plays-an-important-role-in-defense-against-oxidative-stress
#3
Tao Su, Meiru Si, Yunfeng Zhao, Yan Liu, Shumin Yao, Chengchuan Che, Can Chen
Peroxiredoxin Q (PrxQ) that belonged to the cysteine-based peroxidases has long been identified in numerous bacteria, but the information on the physiological and biochemical functions of PrxQ remain largely lacking in Corynebacterium glutamicum. To better systematically understand PrxQ, we reported that PrxQ from model and important industrial organism C. glutamicum, encoded by the gene ncgl2403 annotated as a putative PrxQ, played important roles in adverse stress resistance. The lack of C. glutamicum prxQ gene resulted in enhanced cell sensitivity, increased ROS accumulation, and elevated protein carbonylation levels under adverse stress conditions...
2018: PloS One
https://www.readbyqxmd.com/read/29429808/immune-parameters-to-p67c-antigen-adjuvanted-with-isa206vg-correlate-with-protection-against-east-coast-fever
#4
Anna Lacasta, Stephen Mwalimu, Elisabeth Kibwana, Rosemary Saya, Elias Awino, Thomas Njoroge, Jane Poole, Nicholas Ndiwa, Roger Pelle, Vishvanath Nene, Lucilla Steinaa
East Coast fever (ECF) is a lymphoproliferative disease caused by the tick-transmitted protozoan parasite Theileria parva. ECF is one of the most serious cattle tick-borne diseases in Sub-Saharan Africa. We have previously demonstrated that three doses of the C-terminal part of the sporozoite protein p67 (p67C) adjuvanted with ISA206VG confers partial protection against ECF at a herd level. We have tested the efficacy of two doses of this experimental vaccine, as reducing the vaccination regimen would facilitate its deployment in the field...
February 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29427748/the-non-thyroidal-illness-syndrome-is-associated-with-postoperative-surgical-site-infections-in-enterocutaneous-fistulae
#5
Huajian Ren, Jianan Ren, Gefei Wang, Zhiwu Hong, Guosheng Gu, Jun Chen, Xiuwen Wu, Qiongyuan Hu, Guanwei Li
BACKGROUND: Non-thyroidal illness syndrome (NTIS) is common in critically ill patients and associated with adverse outcomes. Many enterocutaneous fistula (ECF) patients still suffer NTIS prior to definitive surgery. This study was designed to explore the association between preoperative NTIS and postoperative outcomes in ECF patients. METHODS: A total of 264 ECF patients who underwent definitive surgery from April 2014 to November 2016 were studied. Thyroid hormones were tested for each patient before surgery, and the patients were divided into two groups (NTIS group and euthyroid group) according to the presence of NTIS...
February 7, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29383245/spontaneous-closure-of-multiple-enterocutaneous-fistula-due-to-abdominal-tuberculosis-using-negative-pressure-wound-therapy-a-case-report
#6
Yuliardy Limengka, Wifanto S Jeo
Enterocutaneous fistula (ECF) is one of the most challenging abdominal complications, for surgeons and other healthcare members, which involves significant morbidity and potentially mortality. Despite advancements in both operative and non-operative therapy, fistula-related complications are still unavoidable. Negative pressure wound therapy (NPWT) had been used years to treat chronic wound, to decrease tissue edema, improve circulation, promote healthy granulation tissue and inhibit bacterial growth. We report a 29-year-old male with complicated ECF due to abdominal tuberculosis, that was healed after treated using NPWT...
January 2018: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/29376335/perfluoroalkyl-acids-including-isomers-in-tree-barks-from-a-chinese-fluorochemical-manufacturing-park-implication-for-airborne-transportation
#7
Hangbiao Jin, Guo Qiang Shan, Lingyan Zhu, Hongwen Sun, Yi Luo
Measurement of airborne perfluoroalkyl acids (PFAAs) is challenging, but important for understanding their atmospheric transport. Tree bark is a good media for monitoring semi-volatile compounds in the atmosphere. Whether it could work as an indicator of airborne PFAAs was firstly examined in this study. Bark and leaf samples collected around a Chinese fluorochemical manufacturing park (FMP) were analyzed for PFAAs and their branched isomers. Total PFAA concentrations (∑PFAAs) in the bark (mean, 279 ng/g dw) and leaf (250 ng/g dw) samples were comparable...
January 27, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29358498/signal-peptidase-is-necessary-and-sufficient-for-site-1-cleavage-of-rsiv-in-bacillus-subtilis-in-response-to-lysozyme
#8
Ana N Castro, Lincoln T Lewerke, Jessica L Hastie, Craig D Ellermeier
Extra Cytoplasmic Function (ECF) σ factors are a diverse family of alternative σ factors that allow bacteria to sense and respond to changes in the environment. σV is an ECF σ factor found primarily in low GC Gram-positive bacteria and is required for lysozyme resistance in several opportunistic pathogens. In the absence of lysozyme, σV is inhibited by the anti-σ factor RsiV. In response to lysozyme, RsiV is degraded via the process of Regulated Intramembrane Proteolysis (RIP). RIP is initiated by cleavage of RsiV at site-1 which allows the intramembrane protease RasP to cleave RsiV within the transmembrane domain at site-2 and leads to activation of σV Previous work suggested that RsiV is cleaved by signal peptidase at site-1...
January 22, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29351721/myeloperoxidase-and-neurological-disorder-a-cross-talk
#9
Kanta Pravalika, Deepaneeta Sarmah, Harpreet Kaur, Madhuri Wanve, Jackson Saraf, Kiran Kalia, Anupom Borah, Dileep R Yavagal, Kunjan R Dave, Pallab Bhattacharya
Myeloperoxidase (MPO) is a protein present in azurophilic granules, macrophages, and neutrophils that are released into extracellular fluid (ECF) during inflammation. MPO releases hypochlorous acid (HOCL) and other chlorinated species. It is derived from hydrogen peroxide (H2O2) showing response during inflammatory conditions and plays a role in the immune defense against pathogens. MPO may show unwanted effects by indirectly increasing the formation of reactive nitrogen species (RNS), reactive oxygen species (ROS), and tumor necrosis factor alpha (TNF-α) leading to inflammation and oxidative stress...
January 19, 2018: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29343670/anti-sigma-factor-mediated-cell-surface-stress-responses-in-bacillus-subtilis
#10
Kei Asai
Proteins belonging to the sigma factor family in eubacteria initiate transcription by associating with RNA polymerase. A subfamily, the extracytoplasmic function (ECF) sigma factors, which form a widely distributed bacterial signal transduction system comprising a sigma factor and a cognate membrane-embedded anti-sigma factor, regulates genes in response to stressors that threaten cell envelope integrity including the cell wall and membrane. The Gram-positive soil bacterium Bacillus subtilis provides a valuable model for investigation of the ECF sigma factors...
January 17, 2018: Genes & Genetic Systems
https://www.readbyqxmd.com/read/29343295/cytotoxic-t-lymphocytes-from-cattle-sharing-the-same-mhc-class-i-haplotype-and-immunized-with-live-theileria-parva-sporozoites-differ-in-antigenic-specificity
#11
Lucilla Steinaa, Nicholas Svitek, Elias Awino, Rosemary Saya, Philip Toye
OBJECTIVES: The objective of this study was to assess whether cytotoxic T cells (CTL) generated by the live vaccine, known as "ITM Muguga cocktail", which is used for the cattle disease East Cost fever (ECF) in Sub-Saharan Africa, showed a broad reactivity against many different strains of the causative parasite Theileria parva. We also assessed whether immune responses were similar in cattle expressing the same MHC class I haplotypes. RESULTS: The antigenic specificity of CTL from MHC class I-matched cattle vaccinated with the Muguga cocktail were different...
January 17, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29333045/in-vitro-antiproliferative-effect-of-earthworm-coelomic-fluid-of-eudrilus-eugeniae-eisenia-foetida-and-perionyx-excavatus-on-squamous-cell-carcinoma-9-cell-line-a-pilot-study
#12
Dominic Augustine, Roopa S Rao, Jayaraman Anbu, K N Chidambara Murthy
Introduction: The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present in vitro study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9. Materials and Methods: ECF was collected from the species Eudrilus eugeniae (EE), Eisenia foetida (EF), and Perionyx excavatus (PE) stored at -80°C...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29309626/examining-the-stability-of-the-hospital-anxiety-and-depression-scale-factor-structure-in-adolescents-and-young-adults-with-cystic-fibrosis-a-confirmatory-factor-analysis
#13
Estefany Saez-Flores, Niza A Tonarely, David H Barker, Alexandra L Quittner
Objective: The Hospital Anxiety and Depression Scale (HADS) is a widely used screening measure of anxiety and depression symptoms. However, prior analyses of the measure have found heterogeneous factor structures and called into question its ability to differentiate between symptoms of anxiety and depression. As part of efforts to implement mental health screening in cystic fibrosis (CF) care, the European Cystic Fibrosis Society (ECFS) and Cystic Fibrosis Foundation (CFF) conducted an international survey of 1,454 CF professionals...
January 3, 2018: Journal of Pediatric Psychology
https://www.readbyqxmd.com/read/29308947/predictive-factors-of-thromboembolic-complications-in-patients-with-esophagogatric-adenocarcinoma-undergoing-preoperative-chemotherapy
#14
George Papaxoinis, Konstantinos Kamposioras, Theodora Germetaki, Jamie M J Weaver, Sofia Stamatopoulou, Magdy Nasralla, Zoe Kordatou, Vikki Owen-Holt, Alan Anthoney, Wasat Mansoor
BACKGROUND: Thromboembolic events (TEEs) represent a significant treatment and disease complication for cancer patients. In the present study we assessed the incidence of TEEs in patients receiving preoperative chemotherapy for esophagogastric adenocarcinoma. The risk factors for TEE development and their impact on prognosis were further analyzed. MATERIAL AND METHODS: Data from 590 patients with esophagogastric adenocarcinoma, who received preoperative epirubicin-cisplatin with capecitabine (ECX) or 5-fluorouracil (ECF) between 2009 and 2016 in three UK hospitals were retrospectively collected...
January 8, 2018: Acta Oncologica
https://www.readbyqxmd.com/read/29307693/osteoporotic-vertebral-fracture-prevalence-in-elderly-chinese-men-and-women-a-comparison-of-endplate-cortex-fracture-based-and-morphometrical-deformity-based-methods
#15
Min Deng, Xian Jun Zeng, Lai-Chang He, Jason C S Leung, Anthony W L Kwok, James F Griffith, Timothy Kwok, Ping Chung Leung, Yì Xiáng J Wáng
This study aims to evaluate endplate/cortex fracture (ECF)-based method for detecting osteoporotic vertebral fracture (VF) in elderly Chinese population (age ≥ 65 years). The radiographs of 1954 elderly Chinese men (mean: 72.3 years) and 1953 elderly Chinese women (mean: 72.5 years) were evaluated according to Genant's morphometrical vertebral deformity (VD) severity criteria, as well as identified VF according to ECF without necessary requirement of VD. According to ECF, grade-1, -2, and -3 VF prevalence was 1...
December 2, 2017: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/29299362/tumor-response-evaluation-after-neoadjuvant-chemotherapy-in-locally-advanced-gastric-adenocarcinoma-a-prospective-multi-center-cohort-study
#16
Pietro Achilli, Paolo De Martini, Marco Ceresoli, Giulio M Mari, Andrea Costanzi, Dario Maggioni, Raffaele Pugliese, Giovanni Ferrari
Background: To verify the prognostic value of the pathologic and radiological tumor response after neoadjuvant chemotherapy in the treatment of locally advanced gastric adenocarcinoma. Methods: A total of 67 patients with locally advanced gastric cancer (clinical ≥ T2 or nodal disease and without evidence of distant metastases) underwent perioperative chemotherapy (ECF or ECX regimen) from December 2009 through June 2015 in two surgical units. Histopathological and radiological response to chemotherapy were evaluated by using tumor regression grade (TRG) (Becker's criteria) and volume change assessed by CT...
December 2017: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29278144/a-functional-general-stress-response-of-bradyrhizobium-diazoefficiens-is-required-for-early-stages-of-host-plant-infection
#17
Raphael Ledermann, Ilka Bartsch, Barbara Müller, Janine Wülser, Hans-Martin Fischer
Phylogenetically diverse bacteria respond to various stress conditions by mounting a general stress response (GSR) resulting in the induction of protecting or damage repair functions. In α-proteobacteria, the GSR is induced by a regulatory cascade consisting of the extracytoplasmic function (ECF) σ factor σEcfG, its anti-σ factor NepR and the anti-anti-σ factor PhyR. We have reported previously that σEcfG and PhyR of Bradyrhizobium diazoefficiens (formerly named Bradyrhizobium japonicum), the nitrogen-fixing root nodule symbiont of soybean and related legumes, are required for efficient symbiosis, however, the precise role of the GSR remained undefined...
December 26, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29258832/characterization-of-the-theileria-parva-sporozoite-proteome
#18
James Nyagwange, Edwin Tijhaar, Nicola Ternette, Fredrick Mobegi, Kyle Tretina, Joana C Silva, Roger Pelle, Vishvanath Nene
East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T...
December 16, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/29244862/application-of-system-dynamics-and-participatory-spatial-group-model-building-in-animal-health-a-case-study-of-east-coast-fever-interventions-in-lundazi-and-monze-districts-of-zambia
#19
Chisoni Mumba, Eystein Skjerve, Magda Rich, Karl M Rich
East Coast Fever (ECF) is the most economically important production disease among traditional beef cattle farmers in Zambia. Despite the disease control efforts by the government, donors, and farmers, ECF cases are increasing. Why does ECF oscillate over time? Can alternative approaches such as systems thinking contribute solutions to the complex ECF problem, avoid unintended consequences, and achieve sustainable results? To answer these research questions and inform the design and implementation of ECF interventions, we qualitatively investigated the influence of dynamic socio-economic, cultural, and ecological factors...
2017: PloS One
https://www.readbyqxmd.com/read/29229473/cystic-fibrosis-research-topics-featured-at-the-14th-ecfs-basic-science-conference-chairman-s-summary
#20
Marcus A Mall, Tzyh-Chang Hwang, Ineke Brakman
In recent years, tremendous progress has been made in the development of novel drugs targeting the basic defect in patients with cystic fibrosis (CF). This breakthrough is based on a solid foundation of knowledge on CFTR's function in health and how mutations in CFTR cause CF multi-organ disease. This knowledge has been collected and continuously expanded by an active and persistent CF research community and has paved the way for precision medicine for CF. Since 2004, the European Cystic Fibrosis Society (ECFS) has held an annual Basic Science Conference that has evolved as an international forum for interdisciplinary discussion of hot topics and unsolved questions related to CF research...
December 8, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
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