keyword
MENU ▼
Read by QxMD icon Read
search

C. Difficile

keyword
https://www.readbyqxmd.com/read/29676573/tiacumicin-congeners-with-improved-antibacterial-activity-from-a-halogenase-inactivated-mutant
#1
Haibo Zhang, Xiaoxing Tian, Xiaohui Pu, Qingbo Zhang, Wenjun Zhang, Changsheng Zhang
Tiacumicin B (1, also known as fidaxomicin or difimicin) is a marketed drug for the treatment of Clostridium difficile infections. The biosynthetic pathway of 1 has been studied in Dactylosporangium aurantiacum subsp. hamdenensis NRRL 18085 and has enabled the identification of TiaM as a tailoring dihalogenase. Herein we report the isolation, structure elucidation, and bioactivity evaluation of 14 tiacumicin congeners (including 11 new ones) from the tiaM-inactivated mutant. A new tiacumicin congener, 3, with a propyl group at C-7‴ of the aromatic ring was found to exhibit improved antibacterial activity...
April 20, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29675852/effects-of-natural-products-on-several-stages-of-the-spore-cycle-of-clostridium-difficile-in-vitro
#2
Niloufar Roshan, Thomas V Riley, Katherine A Hammer
AIMS: To investigate the effect of natural products on the spore cycle of C. difficile in vitro. METHODS AND RESULTS: Twenty-two natural products were investigated using four C. difficile strains. Effects on sporulation, determined using microscopy and a conventional spore recovery assay, showed that fresh onion bulb extract (6.3% v v-1 ) and coconut oil (8% v v-1 ) inhibited sporulation in all four isolates by 66-86% and 51-88%, respectively, compared to untreated controls...
April 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29674636/cationic-amphiphilic-bolaamphiphile-based-delivery-of-antisense-oligonucleotides-provides-a-potentially-microbiome-sparing-treatment-for-c-difficile
#3
Arun K Sharma, Jacek Krzeminski, Volkmar Weissig, John P Hegarty, David B Stewart
Conventional antibiotics for C. difficile infection (CDI) have mechanisms of action without organismal specificity, potentially perpetuating the dysbiosis contributing to CDI, making antisense approaches an attractive alternative. Here, three (APDE-8, CODE-9, and CYDE-21) novel cationic amphiphilic bolaamphiphiles (CABs) were synthesized and tested for their ability to form nano-sized vesicles or vesicle-like aggregates (CABVs), which were characterized based on their physiochemical properties, their antibacterial activities, and their toxicity toward colonocyte (Caco-2) cell cultures...
April 19, 2018: Journal of Antibiotics
https://www.readbyqxmd.com/read/29674329/regional-variability-in-fecal-microbiota-transplantation-practices-a-survey-of-the-southern-ontario-fecal-microbiota-transplantation-movement
#4
Susy S Hota, Salman Surangiwala, Aimee S Paterson, Bryan Coburn, Susan M Poutanen
BACKGROUND: There is growing evidence that fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridium difficile infection, but little guidance exists for implementation of FMT programs. The objective of this study is to describe the program characteristics and protocols of 9 planned or operating FMT programs in the Southern Ontario Fecal Microbiota Transplantation (SOFT) Movement, to help guide future FMT program implementation. METHODS: A 59-item survey was administered electronically to clinical leads of the SOFT Movement on June 2, 2016...
April 18, 2018: CMAJ Open
https://www.readbyqxmd.com/read/29672211/increased-prevalence-of-pathogenic-bacteria-in-the-gut-microbiota-of-infants-at-risk-of-developing-celiac-disease-the-proficel-study
#5
Marta Olivares, Alfonso Benítez-Páez, Giada de Palma, Amalia Capilla, Esther Nova, Gemma Castillejo, Vicente Varea, Ascensión Marcos, José Antonio Garrote, Isabel Polanco, Ester Donat, Carmen Ribes-Koninckx, Carmen Calvo, Luis Ortigosa, Francesc Palau, Yolanda Sanz
Celiac disease (CD) is an immune-mediated enteropathy involving genetic and environmental factors, whose interaction influences disease risk. The intestinal microbiota, including viruses and bacteria, could play a role in the pathological process leading to gluten intolerance. In this study, we investigated the prevalence of pathogens in the intestinal microbiota of infants at familial risk of developing CD. We included 127 full-term newborns with at least one first-degree relative with CD. Infants were classified according to milk-feeding practice (breastfeeding or formula feeding) and HLA-DQ genotype (low, intermediate or high genetic risk)...
April 19, 2018: Gut Microbes
https://www.readbyqxmd.com/read/29670381/molecular-epidemiology-of-clostridium-difficile-in-two-tertiary-care-hospitals-in-shandong-province-china
#6
Ying Luo, Wen Zhang, Jing-Wei Cheng, Meng Xiao, Gui-Rong Sun, Cheng-Jie Guo, Ming-Jun Liu, Pei-Shan Cong, Timothy Kudinha
Purpose: The incidence and severity of Clostridium difficile infection (CDI) have markedly increased over the past decade. However, there is very limited epidemiological data on CDI in China so far, specifically no data in Shandong Province. The aim of this study was to evaluate diagnostic algorithm for CDI and to gain data on molecular epidemiology of CDI in the Shandong Province of China. Materials and methods: Nonrepetitive unformed fecal specimens (n=504) were investigated by the glutamate dehydrogenase (GDH), C...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29667487/pre-colonization-with-the-commensal-fungus-candida-albicans-reduces-murine-susceptibility-to-clostridium-difficile-infection
#7
Laura Markey, Lamyaa Shaban, Erin R Green, Katherine P Lemon, Joan Mecsas, Carol A Kumamoto
Clostridium difficile is a major nosocomial pathogen responsible for close to half a million infections and 27,000 deaths annually in the U.S. Preceding antibiotic treatment is a major risk factor for C. difficile infection (CDI) leading to recognition that commensal microbes play a key role in resistance to CDI. Current antibiotic treatment of CDI is only partially successful due to a high rate of relapse. As a result, there is interest in understanding the effects of microbes on CDI susceptibility to support treatment of patients with probiotic microbes or entire microbial communities (e...
April 18, 2018: Gut Microbes
https://www.readbyqxmd.com/read/29666289/new-york-city-house-mice-mus-musculus-as-potential-reservoirs-for-pathogenic-bacteria-and-antimicrobial-resistance-determinants
#8
Simon H Williams, Xiaoyu Che, Ashley Paulick, Cheng Guo, Bohyun Lee, Dorothy Muller, Anne-Catrin Uhlemann, Franklin D Lowy, Robert M Corrigan, W Ian Lipkin
House mice ( Mus musculus ) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detection of pathogenic bacteria and antimicrobial resistance genes by molecular methods. Four hundred sixteen mice were collected from predominantly residential buildings in seven sites across New York City over a period of 13 months...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29665558/prevalence-of-clostridium-difficile-infection-in-hospitalized-patients-with-diarrhoea-results-of-a-polish-multicenter-prospective-biannual-point-prevalence-study
#9
Hanna Pituch, Piotr Obuch-Woszczatyński, Dominika Lachowicz, Robert Kuthan, Katarzyna Dzierżanowska-Fangrat, Agnieszka Mikucka, Katarzyna Jermakow, Aleksandra Pituch-Zdanowska, Kerrie Davies
PURPOSE: We aimed to measure the underdiagnosis of Clostridium difficile infection across Poland and the distribution of PCR-ribotypes of C. difficile. MATERIAL AND METHODS: Twenty seven Polish healthcare facilities (HCFs) participated in this prospective study. Each HCF systematically sent all diarrhoeal stools received from inpatients at their laboratories on two days (one in January 2013 and one in July 2013), independently of CDI test request, to the National Coordinating Laboratory (NCL) for standardized testing of CDI...
April 13, 2018: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29659753/patient-and-strain-characteristics-associated-with-clostridium-difficile-transmission-and-adverse-outcomes
#10
Jessica S H Martin, David W Eyre, Warren N Fawley, David Griffiths, Kerrie Davies, Damian P C Mawer, Timothy E A Peto, Derrick W Crook, A Sarah Walker, Mark H Wilcox
Background: No previous study has used whole-genome sequencing (WGS) to investigate risk factors for C. difficile(CD) transmission between symptomatic cases, or assessed the impact of recent acquisition on patient outcome. Methods: This retrospective cohort study included consecutive diarrhoeal samples positive by cytotoxin assay in Leeds, UK(Aug10-Apr12). All samples underwent culture, ribotyping and WGS(Illumina). Sequenced isolates were compared using single nucleotide variants(SNVs)...
April 12, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29659747/two-distinct-patterns-of-clostridium-difficile-diversity-across-europe-indicates-contrasting-routes-of-spread
#11
David W Eyre, Kerrie A Davies, Georgina Davis, Warren N Fawley, Kate E Dingle, Nicola De Maio, Andreas Karas, Derrick W Crook, Tim E A Peto, A Sarah Walker, Mark H Wilcox
Background: Rates of Clostridium difficile infection vary widely across Europe, as do prevalent ribotypes. The extent of Europe-wide diversity within each ribotype is however unknown. Methods: Inpatient diarrhoeal faecal samples submitted on one day in summer and winter (2012-2013) to laboratories in 482 European hospitals were cultured for C. difficile, and isolates ribotyped; those from the 10 most prevalent ribotypes were Illumina whole-genome sequenced. Pairwise single nucleotide differences (SNPs) were obtained from recombination-corrected maximum-likelihood phylogenies...
April 6, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29655966/secular-trends-in-the-epidemiology-of-clostridium-difficile-infection-cdi-at-a-tertiary-care-hospital-in-barcelona-2006-2015-a-prospective-observational-study
#12
Thais Larrainzar-Coghen, Dolors Rodríguez-Pardo, Nuria Fernández-Hidalgo, Mireia Puig-Asensio, Carles Pigrau, Carmen Ferrer, Virginia Rodríguez, Rosa Bartolomé, David Campany, Benito Almirante
OBJECTIVE: Describe secular trends in the epidemiology and outcome of Clostridium difficile infection (CDI) at a tertiary hospital. METHODS: All consecutive primary CDI episodes in adults (January 2006-December 2015) were included. CDI was diagnosed on the presence of diarrhoea and a positive stool test for C. difficile toxin A and/or B. To define trends, a time-series analysis was performed using yearly data on demographics, clinical characteristics, management, antimicrobial treatment, and outcome of CDI...
April 12, 2018: Anaerobe
https://www.readbyqxmd.com/read/29655671/overreporting-healthcare-associated-c-difficile-a-comparison-of-nhsn-labid-with-clinical-surveillance-definitions-in-the-era-of-molecular-testing
#13
Kathryn Albert, Barbara Ross, David P Calfee, Matthew S Simon
BACKGROUND: Clostridium difficile infection (CDI) is the most common healthcare-associated gastrointestinal infection. Hospitals are required to report cases of healthcare facility-onset CDI (HO-CDI) using the National Healthcare Safety Network's CDI laboratory-identified (LabID) event definition. The aim of this study was to determine the extent of potential over-reporting due to the exclusion of important clinical data within LabID reporting definitions. METHODS: In 2015, retrospective chart review was performed on 212 HO-CDI cases reported from a large urban medical center...
April 11, 2018: American Journal of Infection Control
https://www.readbyqxmd.com/read/29650357/clinical-outcomes-of-clostridium-difficile-infection-according-to-strain-type-a-prospective-study-in-medical-wards
#14
S Serafino, D Consonni, M Migone De Amicis, F Sisto, G Domeniconi, S Formica, M Zarantonello, A Maraschini, M D Cappellini, P Spigaglia, F Barbanti, S Castaldi, G Fabio
OBJECTIVES: To describe clinical characteristics and outcome of Clostridium difficile infection (CDI) patients in Internal Medicine, to identify ribotypes (RTs); to evaluate the association between RT and patient clinical characteristics and report outcome. METHODS: One year prospective cohort study. Clinical data, Barthel Index (BI) and outcomes were collected for all inpatients suffering from CDI (n = 148) in hospital wards in Northern Italy. 84 fecal samples were analysed for molecular typing...
April 9, 2018: European Journal of Internal Medicine
https://www.readbyqxmd.com/read/29649308/subtyping-of-clostridium-difficile-pcr-ribotypes-591-106-and-002-the-dominant-strain-types-circulating-in-medellin-colombia
#15
Clara Lina Salazar, Catalina Reyes, Astrid Vanessa Cienfuegos-Gallet, Emma Best, Santiago Atehortua, Patricia Sierra, Margarita M Correa, Warren N Fawley, Daniel Paredes-Sabja, Mark Wilcox, Angel Gonzalez
We aimed to achieve a higher typing resolution within the three dominant Clostridium difficile ribotypes (591,106 and 002) circulating in Colombia. A total of 50 C. difficile isolates we had previously typed by PCR-ribotyping, representing the major three ribotypes circulating in Colombia, were analyzed. Twenty-seven isolates of ribotype 591, 12 of ribotype 106 and 11 of ribotype 002 were subtyped by multiple locus variable-number tandem-repeat analysis (MLVA). The presence of the PaLoc genes (tcdA/tcdB), toxin production in culture and antimicrobial susceptibility were also determined...
2018: PloS One
https://www.readbyqxmd.com/read/29644943/reduced-clostridium-difficile-tests-and-laboratory-identified-events-with-a-computerized-clinical-decision-support-tool-and-financial-incentive
#16
Gregory R Madden, Ian German Mesner, Heather L Cox, Amy J Mathers, Jason A Lyman, Costi D Sifri, Kyle B Enfield
We hypothesized that a computerized clinical decision support tool for Clostridium difficile testing would reduce unnecessary inpatient tests, resulting in fewer laboratory-identified events. Census-adjusted interrupted time-series analyses demonstrated significant reductions of 41% fewer tests and 31% fewer hospital-onset C. difficile infection laboratory-identified events following this intervention.Infect Control Hosp Epidemiol 2018;1-4.
April 12, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29644517/systemic-inflammatory-responses-in-ulcerative-colitis-patients-and-clostridium-difficile-infection
#17
Julajak Limsrivilai, Krishna Rao, Ryan W Stidham, Shail M Govani, Akbar K Waljee, Andrew Reinink, Laura Johnson, Emily Briggs, Peter D R Higgins
BACKGROUND/AIMS: Finding differences in systemic inflammatory response in ulcerative colitis (UC), UC with Clostridium difficile infection (CDI), and CDI could lead to a better ability to differentiate between UC with symptomatic CDI and UC with C. difficile colonization, and could identify specific inflammatory pathways for UC or CDI, which could be therapeutic targets. METHODS: We prospectively collected sera from symptomatic UC patients whose stools were tested for toxigenic C...
April 12, 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29643194/quantitative-thresholds-enable-accurate-identification-of-clostridium-difficile-infection-by-the-luminex-xtag-gi-pathogen-panel
#18
Sixto M Leal, Elena B Popowitch, Kara J Levinson, Teny M John, Bethany Lehman, Maria Bueno Rios, Peter H Gilligan, Melissa B Miller
Clostridium difficile colonizes the gastrointestinal tract resulting in either asymptomatic carriage or a spectrum of diarrheal illness. If clinical suspicion for C. difficile is low, stool samples are often submitted for analysis by multiplex molecular assays capable of detecting multiple GI pathogens and some institutions do not report this organism due to concerns for high false positive rates. Since clinical disease correlates with organism burden and molecular assays yield quantitative data, we hypothesized that numerical cutoffs could be utilized to improve the specificity of the Luminex xTAG GI pathogen panel (GPP) for C...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29642570/enhanced-efficacy-of-high-dose-oral-vancomycin-therapy-in-clostridium-difficile-diarrhea-for-hospitalized-adults-not-responsive-to-conventional-oral-vancomycin-therapy-antibiotic-stewardship-implications
#19
Burke A Cunha, Julia Sessa, Sharon Blum
Current therapy of Clostridium difficile diarrhea (CDD) is problematic. Optimal treatment for CDD remains oral vancomycin, but there is little data on oral vancomycin dosing regimens. The objective of this C. difficile diarrhea study was to compare the efficacy of "high dose" vancomycin, 500 mg (PO) q6h, as sole treatment and in those who after 72 h failed to respond to conventional doses of oral vancomycin, 125-250 mg (PO) q6h. Hospitalized adults with CDD were evaluated by various oral vancomycin regimens, i...
April 10, 2018: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/29627950/molecular-based-diagnosis-of-clostridium-difficile-infection-is-associated-with-reduced-mortality
#20
Tomer Avni, Tanya Babich, Haim Ben-Zvi, Alaa Atamna, Dafna Yahav, Daniel Shepshelovich, Yaara Leibovici-Weissman, Jihad Bishara
Polymerase chain reaction (PCR) for the diagnosis of Clostridium difficile infection (CDI) might result in overdiagnosis. The clinical outcomes of symptomatic CDI patients diagnosed by PCR remain uncertain. We aimed to determine whether patients whose diagnosis of CDI was based on PCR had different characteristics and clinical outcomes than those diagnosed by toxin immunoassay. Consecutive CDI patients, hospitalized at Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel, between January 2013 and January 2016, were identified retrospectively and included in the study...
April 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
keyword
keyword
3768
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"