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B. subtilis

Waleed Elghwaji, Abeer Mohamed El-Sayed, Kadriya S El-Deeb, Aly M ElSayed
Background: Ferula tingitana L. (Apiaceae) has been considered to have abortive and menstruation-inducing properties. It used to treat sore throat, fever, indigestion, and pains. Objectives: The objective of this study is to establish the chemical composition of the essential oil of flower, leaves of F. tingitana, and to throw light on antimicrobial, cytotoxic activities of Libyan plant. Materials and Methods: The chemical composition of the essential oil of flower (0...
October 2017: Pharmacognosy Magazine
Esti Singer, Yardena Bh Silas, Sigal Ben-Yehuda, Ophry Pines
Fumarase is distributed between two compartments of the eukaryotic cell. The enzyme catalyses the reversible conversion of fumaric to L-malic acid in mitochondria as part of the tricarboxylic acid (TCA) cycle, and in the cytosol/nucleus as part of the DNA damage response (DDR). Here we show that fumarase of the model prokaryote Bacillus subtilis (Fum-bc) is induced upon DNA damage, co-localized with the bacterial DNA and is required for the DDR. Fum-bc can substitute for both eukaryotic functions in yeast. Furthermore, we found that the fumarase dependent intracellular signaling of the B...
November 15, 2017: ELife
Osman Gul, Furkan Turker Saricaoglu, Aysegul Besir, Ilyas Atalar, Fehmi Yazici
Hazelnut meal protein (4% (w/v)) and clove essential oil (CEO) (3% (v/v)) were homogenized with ultrasound (US) at different times (2, 4 and 6 min) and amplitudes (50, 75 and 100%) to obtain nano-emulsion films. Film forming nano-emulsions (FFNs) were analyzed for average particle size (Dz) and zeta potential, and edible film characterization were evaluated depending on US treatment, as well as antibacterial and antioxidant activities. Dz values and zeta potential of FFNs decreased with increasing acoustic energy delivered to nano-emulsion system...
March 2018: Ultrasonics Sonochemistry
Haidong Gu, Xiao Chen, Feng Chen, Xing Zhou, Zohreh Parsaee
This contribution reports the biosynthesis of CuO NPs via ultrasound method using the Cystoseira trinodis extracts as an eco friendly and time saving process. The characterization of cupric oxide NPs was performed using XRD, FE-SEM, EDX, TEM, AFM, photoluminescence, UV-Vis, Raman and FT-IR spectroscopy investigations. SEM images show the spherical structure with the average crystallite size 6nm to 7.8nm of CuO. XRD analysis approved the formation of pure monoclinic crystallite structures of CuO NPs. These observations were confirmed by TEM analysis...
March 2018: Ultrasonics Sonochemistry
Hualiang Pi, John D Helmann
Bacterial cells modulate transcription in response to changes in iron availability. The ferric uptake regulator (Fur) senses intracellular iron availability and plays a central role in maintaining iron homeostasis in Bacillus subtilis Here we utilized FrvA, a high-affinity Fe(2+) efflux transporter from Listeria monocytogenes, as an inducible genetic tool to deplete intracellular iron. We then characterized the responses of the Fur, FsrA, and PerR regulons as cells transition from iron sufficiency to deficiency...
November 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
Mudasir Bashir, Tuba Usmani, Sundaramoorthy Haripriya, Tanveer Ahmed
Three different exudate gums were studied for their in-vitro antioxidant activity, functional and textural properties. In-vitro antioxidant activity of the gum exudates was evaluated in terms of DPPH radical scavenging, hydroxyl radical scavenging, reducing power and phenolic content assays. Among the gum exudates, almond gum exhibited maximum DPPḢ inhibition of 35.52±1.125% followed by apricot gum and gum arabic at the concentration of 1mg/mL. Gum arabic had significantly higher reducing power than the other exudate gums...
November 10, 2017: International Journal of Biological Macromolecules
Shina Hussain, Diann Andrews, Bruce C Hill
The synthesis of cytochrome c oxidase protein from Bacillus subtilis (i.e., BsSCO) binds copper with picomolar affinity, which increases the protein's melting temperature (i.e., TM) by 20 °C. Here two native tryptophans (i.e., W36 and W101) are identified as major contributors to BsSCO's structural form, and their contributions to the stability, intrinsic fluorescence, and copper binding properties of BsSCO are explored. Single mutations of tryptophan to phenylalanine decrease the TM by 10 °C and the folding free energy by 3-4 kcal/mol...
November 17, 2017: Biochemistry
Pattanan Yatip, D Nitin Chandra Teja, Timothy W Flegel, Chumporn Soowannayan
Many bacteria, including Vibrio pathogens of shrimp, need to colonize and/or form biofilms in hosts or the environment to cause disease. Thus, one possible control strategy for shrimp Vibriosis is biofilm inhibition. With this objective, an extract from the Japanese fermented soybean product, Natto was tested with the luminescent shrimp pathogen Vibrio harveyi (VH) for its ability to inhibit or degrade biofilm and to interfere with cell growth in broth. Natto is a traditional fermentation product of Bacillus subtilis var Natto (BSN1)...
November 7, 2017: Fish & Shellfish Immunology
Anna Dragoš, Nivedha Lakshmanan, Marivic Martin, Balázs Horváth, Gergely Maróti, Carolina Falcón García, Oliver Lieleg, Ákos T Kovács
Microbial biofilms are tightly packed, heterogeneous structures that serve as arenas for social interactions. Studies on Gram negative models reveal that during evolution in structured environments like biofilms, isogenic populations commonly diversify into phenotypically and genetically distinct variants. These variants can settle in alternative biofilm niches and develop new types of interactions that greatly influence population productivity. Here, we explore the evolutionary diversification of pellicle biofilms of the Gram positive, spore-forming bacterium Bacillus subtilis...
November 8, 2017: FEMS Microbiology Ecology
Ke Xu Zhou, Nan Li, Graham Christie, D Ian Wilson
The adhesion of spores of 3 Bacillus species with distinctive morphologies to stainless steel and borosilicate glass was studied using the fluid dynamic gauging technique. Marked differences were observed between different species of spores, and also between spores of the same species prepared under different sporulation conditions. Spores of the food-borne pathogen B. cereus were demonstrated to be capable of withstanding shear stresses greater than 1500 Pa when adhered to stainless steel, in contrast to spores of Bacillus subtilis and Bacillus megaterium, which detached in response to lower shear stress...
November 2017: Journal of Food Science
Jingfang Hu, Yueqi Li, Guowei Gao, Shanhong Xia
We have developed a novel mediated biochemical oxygen demand (BOD) biosensor based on immobilized Bacillus subtilis (B. subtilis) on three-dimensional (3D) porous graphene-polypyrrole (rGO-PPy) composite. The 3D porous rGO-PPy composite was prepared using hydrothermal method following with electropolymerization. Then the 3D porous rGO-PPy composite was used as a support for immobilizing negatively charged B. subtilis denoted as rGO-PPy-B through coordination and electrostatic interaction. Further, the prepared rGO-PPy-B was used as a microbial biofilm for establishing a mediated BOD biosensor with ferricyanide as an electronic acceptor...
November 10, 2017: Sensors
Theresa Hölscher, Tina Schiklang, Anna Dragoš, Anne-Kathrin Dietel, Christian Kost, Ákos T Kovács
The competent state is a developmentally distinct phase, in which bacteria are able to take up and integrate exogenous DNA into their genome. Bacillus subtilis is one of the naturally competent bacterial species and the domesticated laboratory strain 168 is easily transformable. In this study, we report a reduced transformation frequency of B. subtilis mutants lacking functional and structural flagellar components. This includes hag, the gene encoding the flagellin protein forming the filament of the flagellum...
November 10, 2017: Environmental Microbiology Reports
Jiajia Zhu, Mingxing Gao, Ruili Zhang, Zhuojian Sun, Chunmei Wang, Fenfang Yang, Tingting Huang, Shaoqi Qu, Li Zhao, Yuwen Li, Zhihui Hao
BACKGROUND: The present study compared the effects of soybean meal fermented by three different probiotics organisms with non-fermented soybean meal on growth performance, serum parameters, immune chemistry and intestinal morphology in weaned piglets. METHODS: One hundred and forty-four 35-day old crossbred (Duroc × Landrace × Yorkshire) piglets were randomly allocated into four different dietary treatments (n = 36 per group) containing 0, 5, 10 and 15% fermented soybean meal...
November 9, 2017: Microbial Cell Factories
Ehsan Mahdinia, Ali Demirci, Aydin Berenjian
Menaquinone-7 (MK-7) is the key form of vitamin K used as a dietary supplement and its production revolves around Bacillus subtilis natto. Current fermentation strategies, which suggest static fermentations without aeration and agitation, can be problematic for large scale MK-7 production due to biofilm formation. The use of biofilm reactors, therefore, is proposed in the present study, which could utilize both agitation and aeration without interrupting MK-7 secretion. In this study, biofilm reactors were constructed using the selected plastic composite support (PCS) and B...
November 8, 2017: Bioprocess and Biosystems Engineering
Philipp F Popp, Mona Dotzler, Jara Radeck, Julia Bartels, Thorsten Mascher
Standardized and well-characterized genetic building blocks allow the convenient assembly of novel genetic modules and devices, ensuring reusability of parts and reproducibility of experiments. In the first Bacillus subtilis-specific toolbox using the BioBrick standard, we presented integrative vectors, promoters, reporter genes and epitope tags for this Gram-positive model bacterium. With the Bacillus BioBrick Box 2.0, we significantly expand the range of our toolbox by providing new integrative vectors, introducing novel tools for fine-tuning protein expression, and carefully evaluating codon-adapted fluorescence proteins in B...
November 8, 2017: Scientific Reports
Fakir Shahidullah Tareq, Hee Jae Shin
LC-MS and NMR spectroscopy guided metabolic profiling and dereplication of a crude extract obtained from the fermentation of a marine-derived bacterium, Bacillus subtilis, followed by chromatographic isolation yielded two new cyclic-lipotetrapeptides, bacilotetrins A (1) and B (2). Based on extensive 1D and 2D NMR and high-resolution ESIMS data analysis, the structures of 1 and 2 were elucidated, revealing the unique structures of these lipopeptides consisting of three leucines and a glutamic acid residue cyclized with a lipophilic 3-hydroxy fatty acid...
November 8, 2017: Journal of Natural Products
Fanjing Meng, Tingtao Chen, Xin Wang, Xiaolei Wang, Hua Wei, Puyuan Tian, Huan Wang, Xiaoxiao Zhao, Liang Shen, Hongbo Xin
Next generation sequencing provides an excellent platform to explore microbiota in any given environment, and little work is required to evaluate the accuracy and sensitivity of high‑throughput sequencing technology. In the present study, a known microbiota containing Escherichia coli, Lactobacillus plantarum, Streptococcus thermophilus, Bifidobacterium bifidum, Bacillus subtilis, Enterococcus faecalis and Salmonella typhimurium was used to evaluate the high‑throughput sequencing technology. The results suggested that there were 122...
October 20, 2017: Molecular Medicine Reports
Kajal Chakraborty, Bini Thilakan, Vinaya Kizhakkepatt Kizhakkekalam
AIMS: Microbiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. METHODS AND RESULTS: A culture-dependent method was used to isolate heterotrophic B. subtilis associated with A. longifolius, and assessed for its antimicrobial properties...
November 6, 2017: Journal of Applied Microbiology
Robert T Gale, Franco K K Li, Tianjun Sun, Natalie C J Strynadka, Eric D Brown
Gram-positive bacteria endow their peptidoglycan with glycopolymers that are crucial for viability and pathogenesis. However, the cellular machinery that executes this function is not well understood. While decades of genetic and phenotypic work have highlighted the LytR-CpsA-Psr (LCP) family of enzymes as cell-wall glycopolymer transferases, their in vitro characterization has been elusive, largely due to a paucity of tools for functional assays. In this report, we synthesized authentic undecaprenyl diphosphate-linked wall teichoic acid (WTA) intermediates and built an assay system capable of monitoring LCP-mediated glycopolymer transfer...
October 12, 2017: Cell Chemical Biology
Seiji Noma, Kazuki Kiyohara, Rina Hirokado, Nami Yamashita, Yuya Migita, Motoharu Tanaka, Soichi Furukawa, Hirokazu Ogihara, Yasushi Morinaga, Noriyuki Igura, Mitsuya Shimoda
The effects of heat treatment (HT), hydrostatic pressure treatment (HPT), and pressurized carbon dioxide treatment (CT) on surface hydrophobicity of B.subtilis 168 spores were investigated. The spore surface hydrophobicity was measured by determining the ratio of hydrophobic spores (RHS) that were partitioned into the n-hexadecane phase from the aqueous spore suspension. The RHS after HT generally increased in a temperature-dependent manner and reached approximately 10% at temperatures above 60°C. The effects of pressurization by HPT and accompanying temperature on increased RHS were complex...
October 26, 2017: Journal of Bioscience and Bioengineering
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