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https://www.readbyqxmd.com/read/28938203/green-synthesis-biological-and-spectroscopic-study-on-the-interaction-of-multi-component-mannich-bases-of-imidazo-2-1-b-benzothiazoles-with-human-serum-albumin
#1
Swetha K Maddili, Leela Prasad Yandrati, Shakuntala Siddam, Vijaya Kumar Kannekanti, Himabindu Gandham
A series of Mannich bases of imidazo[2, l-b]benzothiazoles were prepared through one-pot multi-component reaction in the presence of water as an eco-friendly solvent. All the synthesized compounds were confirmed from IR, (1)HNMR, (13)CNMR, and Mass spectroscopy. Evaluation of in vitro anti-inflammatory and anti-microbial activities of all the synthesized derivatives was further accomplished. These results clearly displayed that compound 6d exhibited outstanding anti-inflammatory activity with a percentage inhibition of 70...
August 19, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28936796/biosurfactants-produced-by-bacillus-subtilis-a1-and-pseudomonas-stutzeri-na3-reduce-longevity-and-fecundity-of-anopheles-stephensi-and-show-high-toxicity-against-young-instars
#2
Punniyakotti Parthipan, Raja Kumaresan Sarankumar, Anitha Jaganathan, Pandian Amuthavalli, Ranganathan Babujanarthanam, Pattanathu K S M Rahman, Kadarkarai Murugan, Akon Higuchi, Giovanni Benelli, Aruliah Rajasekar
Anopheles stephensi acts as vector of Plasmodium parasites, which are responsible for malaria in tropical and subtropical areas worldwide. Currently, malaria management is a big challenge due to the presence of insecticide-resistant strains as well as to the development of Plasmodium species highly resistant to major antimalarial drugs. Therefore, the present study focused on biosurfactant produced by two bacteria Bacillus subtilis A1 and Pseudomonas stutzeri NA3, evaluating them for insecticidal applications against malaria mosquitoes...
September 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28933087/-in-vitro-screening-and-identification-of-plant-growth-promoting-rhizobacteria-against-pathogen-causing-astragalus-root-rot
#3
Fen Gao, Rui Hao, Xue-Mei Qin, Zhen-Hong Lei, Yu-Long Wang, Meng-Liang Wang
The antagonistic effect of Bacillus spp. against Fusarium solani was evaluated by living body dual culture and Oxford cup method. The plant growth promoting properties of those strains that had obvious and stable antifungal activity were then tested. The results showed that the living body and bacteria-free fermentation filtrate of strain G10 both had obvious and stable antifungal effect to F. solani. Besides, the strain possessed such growth promoting properties as phosphate solubilization, nitrogen fixation, and production of IAA, amylase and HCN...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28931947/cytochalasan-and-tyrosine-derived-alkaloids-from-the-marine-sediment-derived-fungus-westerdykella-dispersa-and-their-bioactivities
#4
Dan Xu, Minghe Luo, Fenglou Liu, Dong Wang, Xuejiao Pang, Ting Zhao, Lulin Xu, Xia Wu, Mingyu Xia, Xiaolong Yang
Six new cytochalasans, designated as 18-oxo-19,20-dihydrophomacin C (1), 18-oxo-19-methoxy-19,20- dihydrophomacin C (2), 18-oxo-19-hydroxyl-19,20-dihydrophomacin C (3), 19,20-dihydrophomacin C (4), 19-methoxy-19,20-dihydrophomacin C (5), 19-hydroxyl-19,20-dihydrophomacin C (6), and one new tyrosine-derived alkaloid named as gymnastatin Z (8), together with two known compounds, phomacin B (7) and triticone D (9), were isolated from a solid-substrate fermentation culture of Westerdykella dispersa which was derived from marine sediments...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28930232/antimicrobial-antioxidant-and-cytotoxic-activities-of-ocimum-forskolei-and-teucrium-yemense-lamiaceae-essential-oils
#5
Nasser A Awadh Ali, Bhuwan K Chhetri, Noura S Dosoky, Khola Shari, Ahmed J A Al-Fahad, Ludger Wessjohann, William N Setzer
Background:Ocimum forskolei and Teucrium yemense (Lamiaceae) are used in traditional medicine in Yemen. Methods: The chemical composition, antimicrobial, antioxidant and cytotoxic activities of the essential oils isolated from the leaves of Ocimum forskolei Benth. (EOOF) and two different populations of Teucrium yemense Deflers., one collected from Dhamar province (EOTY-d), and another collected from Taiz (EOTY-t) were investigated. The antimicrobial activities of the oils were evaluated against several microorganisms with the disc diffusion test or the broth microdilution test...
April 1, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28926259/dual-photochemical-reaction-pathway-in-flavin-based-photoreceptor-lov-domain-a-combined-quantum-mechanics-molecular-mechanics-investigation
#6
Setsuko Nakagawa, Oliver Weingart, Christel M Marian
The primary photochemical reaction of the light, oxygen, and voltage (LOV) domain of the blue-light photo-sensor YtvA of Bacillus subtilis were investigated using high-level QM(DFT/MRCI)/MM methods. After blue-light excitation, the Sγ atom of the reactive cysteine forms a covalent bond with the C4a of the flavin mononucleotide (FMN) ring. Two conformations for the side chain of reactive cysteine with occupancies of 70% (conf A) and 30% (conf B) are observed in the X-ray crystallographic structures of the YtvA-LOV (Möglich, A...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28920402/-preparation-and-transformation-optimization-for-supercompetent-b-subtilis-sck6-cells
#7
Xinzhi Li, Zhenghui Lu, Yuling Zhou, Shiyu Li, Guimin Zhang
Bacillus subtilis is Gram-positive aerobic bacterium and widely used as a heterologous protein expression host because of its safety and high protein secretion property. However, comparing to Escherichia coli, the low transformation efficiency limits the application of B. subtilis as a host cell for directed evolution of heterologous enzymes. Therefore, we optimized the competent cell preparation conditions for conventional plasmid, including the alteration of the medium, the concentration of inducer, the plasmid type, and other parameters...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28911650/time-course-effects-of-fermentation-on-fatty-acid-and-volatile-compound-profiles-of-cheonggukjang-using-new-soybean-cultivars
#8
Kye Man Cho, Ho-Jeong Lim, Mi-So Kim, Da Som Kim, Chung Eun Hwang, Sang Hae Nam, Ok Soo Joo, Byong Won Lee, Jae Kyeom Kim, Eui-Cheol Shin
In this study, we investigated the effects of the potential probiotic Bacillus subtilis CSY191 on the fatty acid profiles of Cheonggukjang, a fermented soybean paste, prepared using new Korean brown soybean cultivars, protein-rich cultivar (Saedanbaek), and oil-rich cultivar (Neulchan). Twelve fatty acids were identified in the sample set-myristic, palmitic, palmitoleic, stearic, oleic, vaccenic, linoleic, α-linolenic, arachidic, gondoic, behenic, and lignoceric acids-yet, no specific changes driven by fermentation were noted in the fatty acid profiles...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28905232/production-of-d-alanine-from-dl-alanine-using-immobilized-cells-of-bacillus-subtilis-hlz-68
#9
Yangyang Zhang, Xiangping Li, Caifei Zhang, Xiaodong Yu, Fei Huang, Shihai Huang, Lianwei Li, Shiyu Liu
Immobilized cells of Bacillus subtilis HLZ-68 were used to produce D-alanine from DL-alanine by asymmetric degradation. Different compounds such as polyvinyl alcohol and calcium alginate were employed for immobilizing the B. subtilis HLZ-68 cells, and the results showed that cells immobilized using a mixture of these two compounds presented higher L-alanine degradation activity, when compared with free cells. Subsequently, the effects of different concentrations of polyvinyl alcohol and calcium alginate on L-alanine consumption were examined...
September 13, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28900027/sirtuin-lipoamidase-activity-is-conserved-in-bacteria-as-a-regulator-of-metabolic-enzyme-complexes
#10
Elizabeth A Rowland, Todd M Greco, Caroline K Snowden, Anne L McCabe, Thomas J Silhavy, Ileana M Cristea
Lipoic acid is an essential metabolic cofactor added as a posttranslational modification on several multimeric enzyme complexes. These protein complexes, evolutionarily conserved from bacteria to humans, are core regulators of cellular metabolism. While the multistep enzymatic process of adding lipoyl modifications has been well characterized in Escherichia coli, the enzyme required for the removal of these lipoyl moieties (i.e., a lipoamidase or delipoylase) has not yet been identified. Here, we describe our discovery of sirtuins as lipoamidases in bacteria and establish their conserved substrates...
September 12, 2017: MBio
https://www.readbyqxmd.com/read/28899489/enhanced-production-of-xylitol-from-xylose-by-expression-of-bacillus-subtilis-arabinose-h-symporter-and-scheffersomyces-stipitis-xylose-reductase-in-recombinant-saccharomyces-cerevisiae
#11
Hyoju Kim, Hyun-Soo Lee, Haeseong Park, Dae-Hee Lee, Eckhard Boles, Donghwa Chung, Yong-Cheol Park
Inefficient transport of xylose into Saccharomyces cerevisiae is a major hurdle for production of xylitol, a natural sweetener with five carbons. To facilitate the xylose transport and hence increase xylose conversion to xylitol, the araE gene encoding an arabinose:H(+) symporter (AraE) from Bacillus subtilis and the XYL1 gene from Scheffersomyces stipitis were expressed in Saccharomyces cerevisiae EBY.VW4000, a hxt null mutant. The resulting strain of EXHA exhibited 4.1 fold increases in xylose consumption rate and xylitol productivity, relative to the control strain without AraE...
December 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28899391/mixomics-analysis-of-bacillus-subtilis-effect-of-oxygen-availability-on-riboflavin-production
#12
Junlang Hu, Pan Lei, Ali Mohsin, Xiaoyun Liu, Mingzhi Huang, Liang Li, Jianhua Hu, Haifeng Hang, Yingping Zhuang, Meijin Guo
BACKGROUND: Riboflavin, an intermediate of primary metabolism, is one kind of important food additive with high economic value. The microbial cell factory Bacillus subtilis has already been proven to possess significant importance for the food industry and have become one of the most widely used riboflavin-producing strains. In the practical fermentation processes, a sharp decrease in riboflavin production is encountered along with a decrease in the dissolved oxygen (DO) tension. Influence of this oxygen availability on riboflavin biosynthesis through carbon central metabolic pathways in B...
September 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28898061/post-synthetic-modification-of-bacterial-peptidoglycan-using-bioorthogonal-n-acetylcysteamine-analogs-and-peptidoglycan-o-acetyltransferase-b
#13
Yiben Wang, Klare M Lazor, Kristen Elizabeth DeMeester, Hai Liang, Tyler K Heiss, Catherine Leimkuhler Grimes
Bacteria have the natural ability to install protective post-synthetic modifications onto its bacterial peptidoglycan (PG), the coat woven into bacterial cell wall. Peptidoglycan O-acetyltransferase B (PatB) catalyzes the O-acetylation of PG in Gram (-) bacteria, which aids in bacterial survival, as it prevents autolysins such as lysozyme from cleaving the PG. We explored the mechanistic details of PatB's acetylation function and determined that PatB has substrate specificity for bioorthgonal short N-acetyl cysteamine (SNAc) donors...
September 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28894939/identification-and-characterization-of-novel-broad-spectrum-amino-acid-racemases-from-escherichia-coli-and-bacillus-subtilis
#14
Tetsuya Miyamoto, Masumi Katane, Yasuaki Saitoh, Masae Sekine, Hiroshi Homma
The peptidoglycan layer of the bacterial cell wall typically contains D-alanine (D-Ala) and D-glutamic acid (D-Glu), and also various non-canonical D-amino acids that have been linked to peptidoglycan remodeling, inhibition of biofilm formation, and triggering of biofilm disassembly. Bacteria produce D-amino acids when adapting to environmental changes as a common survival strategy. In our previous study, we detected non-canonical D-amino acids in Escherichia coli grown in minimal medium. However, the biosynthetic pathways of non-canonical D-amino acids remain poorly understood...
September 11, 2017: Amino Acids
https://www.readbyqxmd.com/read/28890133/expanding-the-kinome-world-a-new-protein-kinase-family-widely-conserved-in-bacteria
#15
Hien-Anh Nguyen, Takla El Khoury, Sébastien Guiral, Maria-Halima Laaberki, Marie-Pierre Candusso, Frédéric Galisson, Anne-Emmanuelle Foucher, Salsabil Kesraoui, Lionel Ballut, Sylvain Vallet, Cédric Orelle, Laure Zucchini, Juliette Martin, Adeline Page, Jihad Attieh, Nushin Aghajari, Christophe Grangeasse, Jean-Michel Jault
Fine-tuning of signaling pathways is essential for cells to cope with sudden environmental variations. This delicate balance is maintained in particular by protein kinases that control the activity of target proteins by reversible phosphorylation. In addition to homologous eukaryotic enzymes, bacteria have evolved some specific Ser/Thr/Tyr protein kinases without any structural resemblance to their eukaryotic counterparts. Here, we show that a previously identified family of ATPases, broadly conserved among bacteria, is in fact a new family of protein kinases with a Ser/Thr/Tyr kinase activity...
September 7, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28887527/pan-genome-analysis-of-bacillus-for-microbiome-profiling
#16
Yihwan Kim, InSong Koh, Mi Young Lim, Won-Hyong Chung, Mina Rho
Recent advances in high-throughput sequencing technology allow for in-depth studies on microbial genomes and their communities. While multiple strains of the same species could display genomic variations with different gene contents in diverse habitats and hosts, the essential functions for a specific species are conserved as core genes that are shared among strains. We have comprehensively analyzed 238 strains of five different Bacillus species to identify the properties of core and strain-specific genes. Core and strain-specific genes in each Bacillus species show significant differences in their functions and genomic signatures...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28883146/draft-genome-sequence-of-the-gluten-hydrolyzing-bacterium-bacillus-subtilis-gs-188-isolated-from-wheat-sourdough
#17
B S Rashmi, Devaraja Gayathri
The draft genome sequence of Bacillus subtilis GS 188, a novel spore-forming probiotic bacterium with gluten-hydrolyzing potential, was isolated from wheat sourdough and provides deep insights into the beneficial features of this strain for its use in the preparation of gluten-reduced wheat foods for humans with celiac disease.
September 7, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28882201/b-subtilis-as-a-model-for-studying-the-assembly-of-fe-s-clusters-in-gram-positive-bacteria
#18
Patricia C Dos Santos
Complexes of iron and sulfur (Fe-S clusters) are widely distributed in nature and participate in essential biochemical reactions. The biological formation of Fe-S clusters involves dedicated pathways responsible for the mobilization of sulfur, the assembly of Fe-S clusters, and the transfer of these clusters to target proteins. Genomic analysis of Bacillus subtilis and other Gram-positive bacteria indicated the presence of only one Fe-S cluster biosynthesis pathway, which is distinct in number of components and organization from previously studied systems...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28881211/the-in-situ-bacterial-production-of-fluorescent-organic-matter-an-investigation-at-a-species-level
#19
B G Fox, R M S Thorn, A M Anesio, D M Reynolds
Aquatic dissolved organic matter (DOM) plays an essential role in biogeochemical cycling and transport of organic matter throughout the hydrological continuum. To characterise microbially-derived organic matter (OM) from common environmental microorganisms (Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa), excitation-emission matrix (EEM) fluorescence spectroscopy was employed. This work shows that bacterial organisms can produce fluorescent organic matter (FOM) in situ and, furthermore, that the production of FOM differs at a bacterial species level...
August 18, 2017: Water Research
https://www.readbyqxmd.com/read/28878042/interactions-of-the-bacillus-subtilis-dnae-polymerase-with-replisomal-proteins-modulate-its-activity-and-fidelity
#20
Vasileios Paschalis, Emmanuelle Le Chatelier, Matthew Green, François Képès, Panos Soultanas, Laurent Janniere
During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase briefly extends RNA primers synthesized by the primase DnaG prior to handing-off DNA synthesis to PolC. Here, we show in vivo that (i) the polymerase activity of DnaE is essential for both the initiation and elongation stages of DNA replication, (ii) its error rate varies inversely with PolC concentration, and (iii) its misincorporations are corrected by the mismatch repair system post-replication...
September 2017: Open Biology
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