keyword
MENU ▼
Read by QxMD icon Read
search

B. subtilis

keyword
https://www.readbyqxmd.com/read/29792964/a-family-30-glucurono-xylanase-from-bacillus-subtilis-lc9-expression-characterization-and-its-application-in-chinese-bread-making
#1
Yalan Guo, Zhen Gao, Jiaxing Xu, Siyuan Chang, Bin Wu, Bingfang He
A GH30-8 endoxylanase was identified from an environmental Bacillus subtilis isolate following growth selection on aspen wood glucuronoxylan. The putative endoxylanase was cloned for protein expression and characterization in the Gram-positive protease deficient protein expression host B. subtilis WB800. The extracellular activity obtained was 55 U/mL, which was 14.5-fold higher than that obtained with the native species. The apparent molecular mass of BsXyn30 was estimated as 43 kDa by SDS-PAGE. BsXyn30 showed an optimal activity at pH 7...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792927/bacillus-subtilis-as-probiotic-candidate-for-red-sea-bream-growth-performance-oxidative-status-and-immune-response-traits
#2
Amr I Zaineldin, Sayed Hegazi, Shunsuke Koshio, Manabu Ishikawa, Abdulnasser Bakr, Abeer M S El-Keredy, Mahmoud A O Dawood, Serge Dossou, Weilong Wang, Zhang Yukun
The effects of dietary administration of Bacillus subtilis on the growth, digestive enzyme activity, blood chemistry, oxidative status and immune response of red sea bream (Pagrus major) were evaluated in the current study. Fish fed five different levels of B. subtilis at 0 (BS0), 1 × 104 (BS1), 1 × 106 (BS2), 1 × 108 (BS3) and 1 × 1010 (BS4) CFU kg-1 diet for 60 days. The obtained results showed that B. subtilis supplementation significantly improved growth performance (FBW, WG and SGR), feed utilization (FI, FCE, PER and PG) and whole-body protein content when compared to the control group (P < 0...
May 21, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29788229/subtiwiki-in-2018-from-genes-and-proteins-to-functional-network-annotation-of-the-model-organism-bacillus-subtilis
#3
Bingyao Zhu, Jörg Stülke
Living cells are made up of individual parts, i.e. the genome, the proteins, the RNA and lipid molecules as well as the metabolites and ions. However, life depends on the functional interaction among these components which is often organized in networks. Here, we present the recent development of SubtiWiki, the integrated database for the model bacterium Bacillus subtilis (http://subtiwiki.uni-goettingen.de/). SubtiWiki is based on a relational database and provides access to published information about the genes and proteins of B...
January 4, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29785949/-the-functions-of-yhcz-gene-during-bacillus-thuringiensis-growth
#4
Lin da Jia, Tan Tan Gao, Qi Peng, Jing Lv, Jie Zhang, Min Chen, Fu Ping Song
yhcZ and yhcY genes constitute a two-component system in Bacillus subtilis and B. cereus that regulates bacterial growth. However, the exact biological function of yhcZ gene in B. thuringiensis has not been fully elucidated. In this study, we proved that HD73_5824 is an yhcZ gene in B. thuringiensis subsp. kurstaki HD73 strain by combining gene functional annotation, analysis of upstream and downstream genes arrangement, and amino acid sequence alignment. This yhcZ gene may co-regulate bacterial growth with HD73_5825 gene (yhcY gene) by constituting a two-component system...
May 20, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29782978/a-potent-chitinase-from-bacillus-subtilis-for-the-efficient-bioconversion-of-chitin-containing-wastes
#5
Di Wang, Anjie Li, Hongyu Han, Tian Liu, Qing Yang
Bioconversion of chitin-containing waste to N-acetyl-d-glucosamine (GlcNAc) is of economic interest. Highly efficient chitinases are thus desirable. Bacillus subtilis is a well-known microorganism for industrial protein production and biocontrol, however its chitinolytic enzyme has not been studied in detail. In this study, BsChi from B. subtilis was recombinantly expressed in Escherichia coli and characterized as a potent chitinase for degrading crystalline chitin substrates such as α-chitin, β-chitin and crab shells...
May 18, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29780759/para-substituted-functionalised-ferrocene-esters-with-novel-antibacterial-properties
#6
Kevin Muñoz Forti, Faviola Bernard, Gustavo Santiago-Collazo, Waldemar Garcia, Jose L Vera, Enrique Meléndez, Edu B Suarez-Martinez
Introduction: Bacterial antibiotic resistance is on rise despite advances in the development of new antibiotics. In an attempt to circumvent resistance, scientists are shifting focus from modifying existent antibiotics to identifying new antibiotic compounds. Aim: To assess the potential antibiotic effects of functionalised ferrocenecarboxylates para-substituted on the phenoxy pendant group to form: 4-fluorophenyl, 4-chlorophenyl, 4-bromophenyl, 4-iodophenyl and 4-(H-pyrrol-1-yl)phenyl...
February 2018: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29778820/synthesis-of-bioactive-compounds-from-vermicast-isolated-actinomycetes-species-and-its-antimicrobial-activity-against-human-pathogenic-bacteria
#7
R Balachandar, N Karmegam, M Saravanan, R Subbaiya, P Gurumoorthy
The present study aimed to evaluate the antimicrobial activities of bioactive compounds synthesized from vermicast isolated actinomycetes species. Specifically, the synthesized bioactive compounds were evaluated for their antimicrobial activity against selected Gram + ve and Gram-ve human pathogenic bacteria including Staphylococcus aureus, Bacillus circulans, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli. Interestingly, a total of five different actinomycetesspecies were recovered from vermicasts...
May 17, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29777449/potential-of-bacillus-subtilis-lipopeptides-in-anti-cancer-i-induction-of-apoptosis-and-paraptosis-and-inhibition-of-autophagy-in-k562-cells
#8
Haobin Zhao, Lu Yan, Xiaoguang Xu, Chunmei Jiang, Junling Shi, Yawen Zhang, Li Liu, Shuzhen Lei, Dongyan Shao, Qingsheng Huang
The lipopeptide iturin from Bacillus subtilis has been found to have a potential inhibitory effect on breast cancer, alveolar adenocarcinoma, renal carcinoma, and colon adenocarcinoma. In this study, the potential of B. subtilis lipopeptides (a mixture of iturin homologues, concentration of 42.75%) to inhibit chronic myelogenous leukemia was evaluated using K562 myelogenous leukemia cells. The results showed that the lipopeptides could completely inhibit the growth of K562 at 100 μM, with an IC50 value of 65...
May 9, 2018: AMB Express
https://www.readbyqxmd.com/read/29776364/identification-function-and-application-of-3-ketosteroid-%C3%AE-1-dehydrogenase-isozymes-in-mycobacterium-neoaurum-dsm-1381-for-the-production-of-steroidic-synthons
#9
Ruijie Zhang, Xiangcen Liu, Yushi Wang, Yuchang Han, Junsong Sun, Jiping Shi, Baoguo Zhang
BACKGROUND: 3-Ketosteroid-Δ1-dehydrogenase (KstD) is a key enzyme in the metabolic pathway for chemical modifications of steroid hormones. Only a few KstDs have thus far been characterized biochemically and applied for the production of steroidal pharmaceutical intermediates. Three KstDs, KstD1, KstD2, and KstD3, were identified in Mycobacterium neoaurum DSM 1381, and they shared up to 99, 85 and 97% amino acid identity with previously reported KstDs, respectively. In this paper, KstDs from M...
May 18, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29775705/effects-of-different-chemical-modifications-on-the-antibacterial-activities-of-polysaccharides-sequentially-extracted-from-peony-seed-dreg
#10
Xiao-Li Li, Kiran Thakur, Yuan-Yuan Zhang, Xiao-Fang Tu, Ying-Shuo Zhang, Dan-Ye Zhu, Jian-Guo Zhang, Zhao-Jun Wei
The peony seed dreg polysaccharide (PSDPs) fractions and their modified derivatives were examined for their antibacterial potential. PSDPs along with their derivatives exhibited appreciable antibacterial activity against both Gram-positive (B. subtilis and S. aureus) as well as Gram-negative (E. coli and S. typhimurium) bacteria. Among the four polysaccharide fractions, HBSS possessed the greatest antibacterial activity with an inhibition zone of 13.6 mm against S. typhimurium, minimal inhibitory concentration of 0...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775652/advances-and-prospects-of-bacillus-subtilis-cellular-factories-from-rational-design-to-industrial-applications
#11
REVIEW
Yang Gu, Xianhao Xu, Yaokang Wu, Tengfei Niu, Yanfeng Liu, Jianghua Li, Guocheng Du, Long Liu
Bacillus subtilis is the most characterized gram-positive bacterium that has significant attributes, such as growing well on cheap carbon sources, possessing clear inherited backgrounds, having mature genetic manipulation methods, and exhibiting robustness in large-scale fermentations. Till date, B. subtilis has been identified as attractive hosts for the production of recombinant proteins and chemicals. By applying various systems and synthetic biology tools, the productivity features of B. subtilis can be thoroughly analyzed and further optimized via metabolic engineering...
May 15, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29772337/modeling-of-the-bacterial-inactivation-kinetics-of-dialdehyde-cellulose-in-aqueous-suspension
#12
Xihong He, Zhina He, Yan Li, Haifeng Yu, Liming Zhang, Huanhuan Ge, Shuli Man, Yujie Dai
The purpose of this study was to estimate the antibacterial properties of aqueous suspensions of dialdehyde microcrystalline cellulose (DAMC), and a model of the observed antibacterial kinetics was proposed and validated. The DAMC was prepared from microcrystalline cellulose by periodate oxidation. The bacterial inactivation kinetics of aqueous suspensions of DAMC was investigated by measuring the bactericidal effect after different contact times. The results indicated that with a DAMC aldehyde content of 6...
May 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29767043/study-of-bacillus-subtilis-on-growth-performance-nutrition-metabolism-and-intestinal-microflora-of-1-to-42-d-broiler-chickens
#13
Zhenhua Gao, Haohao Wu, Lin Shi, Xiaohui Zhang, Ran Sheng, Fuquan Yin, Ravi Gooneratne
To study the influence of different levels of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d Arbor Acres (AA) broilers, a total of 800 one-day-old healthy broilers were randomly divided into 5 groups with 4 replicates per group and 40 broilers per replicate. Broilers were fed a basic diet (group 1) which acted as the control group, and 4 other groups (2 to 5) were fed the basal diet with B. subtilis added at concentrations of 100, 150, 200 and 250 mg/kg, respectively for 42 days...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766798/high-level-production-of-menaquinone-7-by-milking-with-biocompatible-organic-solvents
#14
Dinali Ranmadugala, Alireza Ebrahiminezhad, Merilyn Manley-Harris, Younes Ghasemi, Aydin Berenjian
The effect of milking menaquinone-7 (MK 7) with biocompatible organic solvents on MK 7 production in B. subtilis fermentation was studied. Periodic milking of MK-7 from the fermentation medium by n-hexane significantly enhanced the total MK 7 production ~ 1.7 fold at the end of 72 hours of fermentation (p < 0.05) as compared to the control medium. Milking of MK 7 with a mixture of n-hexane phase modified with n-butanol was also explored. Although milking of MK-7 by a mixture of n-hexane and n-butanol (1:2, v/v), was found to be appropriate in terms of high extraction capacity, no significant increase in total MK-7 concentration was observed...
May 14, 2018: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/29764195/eremophilane-sesquiterpenes-from-the-endophytic-fungus-xylaria-sp-gdg-102
#15
Yan Liang, Weifeng Xu, Chengmei Liu, Dexiong Zhou, Xiaobo Liu, Yuyue Qin, Fei Cao, Jun Li, Ruiyun Yang, Jiangke Qin
A new eremophilane sesquiterpene, xylareremophil (1), together with five known eremophilanes, 1α,10α-epoxy-3α-hydroxyeremophil-7(11)-en-12,8β-olide (2), 1,10α,13-trihydroxyeremophil-7(11)-en-12,8-olide (3), 1α,10α-epoxy-13-hydroxyeremophil-7(11)-en-12,8β-olide (4), mairetolides B (5) and G (6) were isolated from the endophytic fungus Xylaria sp. GDG-102 cultured from Sophora tonkinensis. Their structures were elucidated on the basis of spectroscopic data analysis. The absolute configurations of 1 was determined by comparing computed electronic circular dichroism (ECD) and optical rotation (OR) with experimental results...
May 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29760236/anti-sigma-factor-ylad-regulates-transcriptional-activity-of-sigma-factor-ylac-and-sporulation-via-manganese-dependent-redox-sensing-molecular-switch-in-bacillus-subtilis
#16
Min-Kyu Kwak, Han-Bong Ryu, Sung-Hyun Song, Jin-Won Lee, Sa-Ouk Kang
YlaD, a membrane-anchored anti-sigma factor of Bacillus subtilis , contains a HX3 CXXC motif that functions as a redox-sensing domain and belongs to one of the zinc-coordinated anti-sigma factor families. Despite previously showing that the YlaC transcription is controlled by YlaD, experimental evidence of how the YlaC-YlaD interaction is affected by active cysteines and/or metal ions is lacking. Here, we showed that the P yla promoter is autoregulated solely by YlaC. Moreover, reduced YlaD contained zinc and iron, while oxidized YlaD did not...
May 14, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29754050/sargassum-wightii-synthesized-zno-nanoparticles-from-antibacterial-and-insecticidal-activity-to-immunostimulatory-effects-on-the-green-tiger-shrimp-penaeus-semisulcatus
#17
Ramachandran Ishwarya, Baskaralingam Vaseeharan, Suganya Subbaiah, Abdul Khudus Nazar, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Mohammed N Al-Anbr
The green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. Here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of Sargassum wightii. The hot water extract prepared from S. wightii (H Sw) and ZnO NPs were studied by UV-visible and FTIR spectroscopy, SEM and XRD. Then, both products were evaluated for antibiofilm activity towards aquatic pathogens. The nanoparticles' immunostimulating potential on green tiger prawns, Penaeus semisulcatus was studied through immersion and dietary administration...
May 3, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29750913/synthesis-biophysical-and-functional-studies-of-two-bp100-analogues-modified-by-a-hydrophobic-chain-and-a-cyclic-peptide
#18
Gustavo P B Carretero, Greice K V Saraiva, Ana C G Cauz, Magali A Rodrigues, Sumika Kiyota, Karin A Riske, Alcindo A Dos Santos, Marcos F Pinatto-Botelho, Marcelo P Bemquerer, Frederico J Gueiros-Filho, Hernan Chaimovich, Shirley Schreier, Iolanda M Cuccovia
Antimicrobial peptides (AMPs) work as a primary defense against pathogenic microorganisms. BP100, (KKLFKKILKYL-NH2 ), a rationally designed short, highly cationic AMP, acts against many bacteria, displaying low toxicity to eukaryotic cells. Previously we found that its mechanism of action depends on membrane surface charge and on peptide-to-lipid ratio. Here we present the synthesis of two BP100 analogs: BP100‑alanyl‑hexadecyl‑1‑amine (BP100-Ala-NH-C16 H33 ) and cyclo(1‑4)‑d‑Cys1 , Ile2 , Leu3 , Cys4 -BP100 (Cyclo(1‑4)‑cILC-BP100)...
May 8, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29748477/genetic-tools-and-techniques-for-recombinant-expression-in-thermophilic-bacillaceae
#19
REVIEW
Eivind B Drejer, Sigrid Hakvåg, Marta Irla, Trygve Brautaset
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins. In particular, for thermostable proteins, there is a need for hosts able to properly synthesize, fold, and excrete these in high yields, and thermophilic Bacillaceae represent one potentially interesting group of microorganisms for such purposes. A number of thermophilic Bacillaceae including B...
May 10, 2018: Microorganisms
https://www.readbyqxmd.com/read/29743855/design-synthesis-characterization-and-computational-docking-studies-of-novel-sulfonamide-derivatives
#20
Hira Saleem, Arooma Maryam, Saleem Ahmed Bokhari, Ayesha Ashiq, Sadaf Abdul Rauf, Rana Rehan Khalid, Fahim Ashraf Qureshi, Abdul Rauf Siddiqi
This study reports three novel sulfonamide derivatives 4-Chloro-N-[(4-methylphenyl) sulphonyl]-N-propyl benzamide ( 1A ), N-(2-hydroxyphenyl)-4-methyl benzene sulfonamide ( 1B ) and 4-methyl-N-(2-nitrophenyl) benzene sulfonamide ( 1C ). The compounds were synthesised from starting material 4-methylbenzenesulfonyl chloride and their structure was studied through 1 H-NMR and 13 C-NMR spectra. Computational docking was performed to estimate their binding energy against bacterial p -amino benzoic acid (PABA) receptor, the dihydropteroate synthase (DHPS)...
2018: EXCLI Journal
keyword
keyword
3767
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"