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B. subtilis

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https://www.readbyqxmd.com/read/27920766/a-degu-p-and-degq-dependent-regulatory-pathway-for-the-k-state-in-bacillus-subtilis
#1
Mathieu Miras, David Dubnau
The K-state in the model bacterium Bacillus subtilis is associated with transformability (competence) as well as with growth arrest and tolerance for antibiotics. Entry into the K-state is determined by the stochastic activation of the transcription factor ComK and occurs in about ∼15% of the population in domesticated strains. Although the upstream mechanisms that regulate the K-state have been intensively studied and are well understood, it has remained unexplained why undomesticated isolates of B. subtilis are poorly transformable compared to their domesticated counterparts...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920297/role-of-ribonucleotide-reductase-on-bacillus-subtilis-stress-associated-mutagenesis
#2
Karla Viridiana Castro-Cerritos, Ronald E Yasbin, Eduardo A Robleto, Mario Pedraza-Reyes
: The Gram-positive microorganism Bacillus subtilis relies on a single class Ib ribonucleotide reductase (RNR) to generate 2' -deoxyribonucleotides (dNDPs) for DNA replication and repair. In this work, we investigated the influence of RNR levels on B. subtilis stationary-phase-associated mutagenesis (SPM). Since RNR is essential in this bacterium, we engineered a conditional mutant in the strain B. subtilis YB955 (hisC952, metB5, leu427) in which expression of the nrdEF operon was modulated by isopropyl-β-D-thiogalactopyranoside (IPTG)...
December 5, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27919757/dietary-administration-of-bacillus-subtilis-hainup40-enhances-growth-digestive-enzyme-activities-innate-immune-responses-and-disease-resistance-of-tilapia-oreochromis-niloticus
#3
Haitian Liu, Shifeng Wang, Yan Cai, Xiaohui Guo, Zhenjie Cao, Yongzheng Zhang, Shubin Liu, Wei Yuan, Weiwei Zhu, Yu Zheng, Zhenyu Xie, Weiliang Guo, Yongcan Zhou
The probiotic properties of Bacillus subtilis HAINUP40 isolated from the aquatic environment, and the effects of dietary administration of B. subtilis HAINUP40 on the growth performance, intestinal probiotic recovery, digestive enzyme activities, innate immunity and disease resistance of tilapia (Oreochromis niloticus) were evaluated. The probiotic properties investigated include tolerance to simulated gastrointestinal stress, auto-aggregation, cell surface hydrophobicity and extracellular enzyme production...
December 2, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27918604/real-time-naked-eye-multiplex-detection-of-toxins-and-bacteria-using-aiegens-with-the-assistance-of-graphene-oxide
#4
Ruoyu Zhang, Xiaolei Cai, Guangxue Feng, Bin Liu
Toxins and bacteria in water or food pose a threat to human life and could potentially be exploited for bioterrorism. Real-time naked-eye detection of these contaminants is highly desirable to provide a direct and simple analytical method and address the challenges of the existing strategies. Using the detection of ricin and B. subtilis as an example, a naked-eye multiplex detection model is established. In this work, a green fluorogen with aggregation-induced emission (AIE) characteristics was encapsulated in silica nanoshells...
December 5, 2016: Faraday Discussions
https://www.readbyqxmd.com/read/27914001/subtilosin-prevents-biofilm-formation-by-inhibiting-bacterial-quorum-sensing
#5
Ammar Algburi, Saskia Zehm, Victoria Netrebov, Anzhelica B Bren, Vladimir Chistyakov, Michael L Chikindas
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27913733/identification-of-2-methylthio-cyclic-n6-threonylcarbamoyladenosine-ms2ct6a-as-a-novel-rna-modification-at-position-37-of-trnas
#6
Byeong-Il Kang, Kenjyo Miyauchi, Michal Matuszewski, Gabriel Silveira D'Almeida, Mary Anne T Rubio, Juan D Alfonzo, Kazuki Inoue, Yuriko Sakaguchi, Takeo Suzuki, Elzbieta Sochacka, Tsutomu Suzuki
Transfer RNA modifications play pivotal roles in protein synthesis. N(6)-threonylcarbamoyladenosine (t(6)A) and its derivatives are modifications found at position 37, 3'-adjacent to the anticodon, in tRNAs responsible for ANN codons. These modifications are universally conserved in all domains of life. t(6)A and its derivatives have pleiotropic functions in protein synthesis including aminoacylation, decoding and translocation. We previously discovered a cyclic form of t(6)A (ct(6)A) as a chemically labile derivative of t(6)A in tRNAs from bacteria, fungi, plants and protists...
December 2, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27910759/acidified-hot-water-extraction-of-adenosine-cordycepin-and-polysaccharides-from-the-chinese-caterpillar-mushroom-ophiocordyceps-sinensis-cs1197-ascomycetes-application-of-an-artificial-neural-network-and-evaluation-of-antioxidant-and-antibacterial-activities
#7
M G Shashidhar, B Manohar
The effects of process variables (temperature, time, and pH) on the extraction of adenosine, cordycepin, and polysaccharides from Ophiocordyceps sinensis CS1197 were studied using response surface methodology (RSM) and an artificial neural network (ANN). The ANN model resulted in low root mean square errors (0.022, 0.079, 0.018) and high R2 values (0.995, 0.934, 0.997) for adenosine, cordycepin, and polysaccharide yields, respectively, which implied good agreement between the predicted and actual data. An overall desirability of 0...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27907199/identification-heterologous-expression-and-functional-characterization-of-bacillus-subtilis-yutf-a-had-superfamily-5-nucleotidase-with-broad-substrate-specificity
#8
Natalia P Zakataeva, Dmitriy V Romanenkov, Yuliya R Yusupova, Victoria S Skripnikova, Takayuki Asahara, Sergey V Gronskiy
5'-nucleotidases (EC 3.1.3.5) catalyze the hydrolytic dephosphorylation of 5'-ribonucleotides and 5'-deoxyribonucleotides as well as complex nucleotides, such as uridine 5'-diphosphoglucose (UDP-glucose), nicotinamide adenine dinucleotide and flavin adenine dinucleotide, to their corresponding nucleosides plus phosphate. These enzymes have been found in diverse species in intracellular and membrane-bound, surface-localized forms. Soluble forms of 5'-nucleotidases belong to the ubiquitous haloacid dehalogenase superfamily (HADSF) and have been shown to be involved in the regulation of nucleotide, nucleoside and nicotinamide adenine dinucleotide (NAD+) pools...
2016: PloS One
https://www.readbyqxmd.com/read/27907152/dietary-probiotic-bacillus-subtilis-strain-fmbj-increases-antioxidant-capacity-and-oxidative-stability-of-chicken-breast-meat-during-storage
#9
Wen Kai Bai, Fei Jing Zhang, Tian Jin He, Peng Wei Su, Xiong Zhi Ying, Li Li Zhang, Tian Wang
This study was aimed to measure the dietary effects of probiotic Bacillus subtilis strain fmbj (BS fmbj) on antioxidant capacity and oxidative stability of chicken breast meat during storage. Treatment groups were fed the basal diet with BS fmbj at 0 g/kg (CON), 0.2 g/kg (BS-1), 0.3 g/kg (BS-2), or 0.4 g/kg (BS-3) doses without antibiotics. During 8 days of storage at 4°C, BS-2 group showed a significant improvement (P < 0.05) on meat quality (pH, Drip loss, Cooking loss, Shear force, color L*, a*, b*), free radical scavenging activity (DPPH, ABTS+, H2O2), tissues antioxidant enzyme capacity (SOD, CAT, GSH-Px, GSH, T-SH), mitochondria antioxidant enzyme capacity (MnSOD, GPx, GSH), mRNA expression of antioxidant genes (Nrf2, HO-1, SOD, CAT, GSH-Px) and mitochondrial function genes (avUCP, NRF1, NRF2, TFAM, PGC-1α), oxidative damage index (MDA, ROS, PC, 8-OHdG), and MMP level in chicken breast meat as compared to the CON group...
2016: PloS One
https://www.readbyqxmd.com/read/27899502/bacillus-subtilis-early-colonization-of-arabidopsis-thaliana-roots-involves-multiple-chemotaxis-receptors
#10
Rosalie Allard-Massicotte, Laurence Tessier, Frédéric Lécuyer, Venkatachalam Lakshmanan, Jean-François Lucier, Daniel Garneau, Larissa Caudwell, Hera Vlamakis, Harsh P Bais, Pascale B Beauregard
: Colonization of plant roots by Bacillus subtilis is mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associated B. subtilis bacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producing B. subtilis is a well-established requirement for long-term colonization. However, we observed that cells start forming a biofilm only several hours after motile cells first settle on the plant...
November 29, 2016: MBio
https://www.readbyqxmd.com/read/27898345/quercetin-loading-cdse-zns-nanoparticles-as-efficient-antibacterial-and-anticancer-materials
#11
Xiaofang Yang, Weiwei Zhang, Zhiwei Zhao, Nuan Li, Zhipeng Mou, Dongdong Sun, Yongping Cai, Weiyun Wang, Yi Lin
Quercetin (Qe) plays an important role in inflammation, antibacterial, anticancer, and aging. However, Qe has extremely low water solubility, which is a major challenge in drug absorption. In this study, we described a simple method for synthesis of Qe/CdSe/ZnS nanoparticles (QCZ NPs). The QCZ NPs had an average diameter of 10nm and prominent yellow emission under UV irradiation. We investigated the antibacterial activity of QCZ NPs against drug-resistant Escherichia coli (E. coli) and Bacillus subtilis (B...
November 17, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27898006/immobilization-of-neutral-protease-from-bacillus-subtilis-for-regioselective-hydrolysis-of-acetylated-nucleosides-application-to-capecitabine-synthesis
#12
Teodora Bavaro, Giulia Cattaneo, Immacolata Serra, Ilaria Benucci, Massimo Pregnolato, Marco Terreni
This paper describes the immobilization of the neutral protease from Bacillus subtilis and its application in the regioselective hydrolysis of acetylated nucleosides, including building blocks useful for the preparation of anticancer products. Regarding the immobilization study, different results have been obtained depending on the immobilization procedure. Epoxy hydrophobic carriers gave a poorly stable derivative that released almost 50% of the immobilized protein under the required reaction conditions. On the contrary, covalent immobilization on a differently activated hydrophilic carrier (agarose) resulted in very stable enzyme derivatives...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27897378/subcellular-clustering-of-a-putative-c-di-gmp-dependent-exopolysaccharide-machinery-affecting-macro-colony-architecture-in-bacillus-subtilis
#13
Patricia Bedrunka, Peter L Graumann
The structure of bacterial biofilms is predominantly established through the secretion of extracellular polymeric substances (EPS). We show that Bacillus subtilis contains an operon (ydaJ-N) whose induction leads to increased Congo Red staining of biofilms and strongly altered biofilm architecture, suggesting that it mediates the production of an unknown exopolysaccharide. Supporting this idea, overproduction of YdaJKLMN leads to cell clumping during exponential growth in liquid culture, and also causes colony morphology alterations in wild type cells, as well as in a mutant background lacking the major exopolysaccharide of B...
November 29, 2016: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/27891125/poly-%C3%AE-glutamic-acids-contribute-to-biofilm-formation-and-plant-root-colonization-in-selected-environmental-isolates-of-bacillus-subtilis
#14
Yiyang Yu, Fang Yan, Yun Chen, Christopher Jin, Jian-Hua Guo, Yunrong Chai
Bacillus subtilis is long known to produce poly-γ-glutamic acids (γ-PGA) as one of the major secreted polymeric substances. In B. subtilis, the regulation of γ-PGA production and its physiological role are still unclear. B. subtilis is also capable of forming structurally complex multicellular communities, or biofilms, in which an extracellular matrix consisting of secreted proteins and polysaccharides holds individual cells together. Biofilms were shown to facilitate B. subtilis-plant interactions. In this study, we show that different environmental isolates of B...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27891124/a-novel-cell-type-enables-b-subtilis-to-escape-from-unsuccessful-sporulation-in-minimal-medium
#15
Hervé Joël Defeu Soufo
Sporulation is the most enduring survival strategy developed by several bacterial species. However, spore development of the model organism Bacillus subtilis has mainly been studied by means of media or conditions optimized for the induction of sporogenesis. Here, I show that during prolonged growth during stationary phase in minimal medium, B. subtilis undergoes an asymmetric cell division that produces small and round-shaped, DNA containing cells. In contrast to wild-type cells, mutants harboring spo0A or spoIIIE/sftA double mutations neither sporulate nor produce this special cell type, providing evidence that the small round cells emerge from the abortion of endospore formation...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27888076/proteome-wide-identification-of-lysine-succinylation-in-thermophilic-and-mesophilic-bacteria
#16
Hiroki Okanishi, Kwang Kim, Kenji Fukui, Takato Yano, Seiki Kuramitsu, Ryoji Masui
Lysine succinylation, one of post-translational acylations conserved from eukaryotes to bacteria, plays regulatory roles in various cellular processes. However, much remains unknown about the general and specific characteristics of lysine succinylation among bacteria, and about its functions different from those of other acylations. In this study, we characterized lysine succinylation, a newly discovered widespread type of lysine acylation in five bacterial species with different characteristics such as optimal growth temperature and cell wall structure...
November 23, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27886094/damxungmacin-a-and-b-two-new-amicoumacins-with-rare-heterocyclic-cores-isolated-from-bacillus-subtilis-xz-7
#17
Hui-Ling Tang, Cheng-Hang Sun, Xin-Xin Hu, Xue-Fu You, Min Wang, Shao-Wei Liu
Two new amicoumacins, named Damxungmacin A (1) and B (2), were isolated from the culture broth of a soil-derived bacterium Bacillus subtilis XZ-7. Their chemical structures were elucidated by spectroscopic studies (UV, IR, NMR and HR-ESI-MS). Compound 1 possessed a 1,4-diazabicyclo[2.2.1]heptane-2-one ring system in its structure, which was reported for the first time, while 2 had a 1-acetylmorpholine-3-one moiety, which was naturally rare. Compound 1 exhibited moderate to weak cytotoxic activities against three human tumor cell lines (A549, HCT116 and HepG2) with IC50 values of 13...
November 23, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27881419/oral-administration-of-a-select-mixture-of-bacillus-probiotics-affects-the-gut-microbiota-and-goblet-cell-function-in-newly-weaned-muc4-resistant-pigs-following-escherichia-coli-challenge
#18
Wei Zhang, Yao-Hong Zhu, Dong Zhou, Qiong Wu, Dan Song, Johan Dicksved, Jiu-Feng Wang
: Structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. Low, moderate, or high doses of a Bacillus licheniformis-B. subtilis mixture (BLS-mix) were orally administered to MUC4 resistant piglets for 1 week before the F4-expressing ETEC strain (F4(+) ETEC) challenge. The luminal contents were collected from the mucosa of colon on day 8 after F4(+) ETEC challenge. BLS-mix attenuated E. coli-induced expansion of Bacteroides uniformis, Eubacterium eligens, Acetanaerobacterium and Sporobacter populations...
November 23, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27878261/bacterial-ghosts-as-adjuvants-in-syngeneic-tumour-cell-lysate-based-anticancer-vaccination-in-a-murine-lung-carcinoma-model
#19
Jan Aleksander Kraśko, Karolina Žilionytė, Adas Darinskas, Marius Strioga, Svetlana Rjabceva, Iosif Zalutsky, Marina Derevyanko, Vladimir Kulchitsky, Werner Lubitz, Pavol Kudela, Edita Miseikyte-Kaubriene, Olha Karaman, Hennadii Didenko, Hryhorii Potebnya, Vasyl Chekhun, Vita Pašukonienė
Instead of relying on external anticancer factors for treatment, immunotherapy utilizes the host's own immune system and directs it against given tumour antigens. This study demonstrated that it is possible to overcome the documented immunosuppressive properties of tumour cell lysate by supplementing it with appropriate adjuvant. Lewis lung carcinoma (LLC)‑challenged C57BL/6 mice were treated with LLC cryo‑lysate mixed with either bacterial ghosts (BGs) generated from E. coli Nissle 1917 or B. subtilis 70 kDa protein as adjuvants...
November 16, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27876054/engineering-an-inducible-gene-expression-system-for-bacillus-subtilis-from-a-strong-constitutive-promoter-and-a-theophylline-activated-synthetic-riboswitch
#20
Wenjing Cui, Laichuang Han, Jintao Cheng, Zhongmei Liu, Li Zhou, Junling Guo, Zhemin Zhou
BACKGROUND: Synthetic riboswitches have been increasingly used to control and tune gene expression in diverse organisms. Although a set of theophylline-responsive riboswitches have been developed for bacteria, fully functional expression elements mediated by synthetic riboswitches in Bacillus subtilis are rarely used because of the host-dependent compatibility between the promoters and riboswitches. RESULTS: A novel genetic element composed of the promoter P43 and a theophylline-riboswitch was developed and characterized in B...
November 22, 2016: Microbial Cell Factories
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