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B. subtilis

Lina María González-Jaramillo, Francisco José Aranda, José Antonio Teruel, Valeska Villegas-Escobar, Antonio Ortiz
Lipopeptide biosurfactants constitute one of the most promising groups of compounds for the treatment and prevention of fungal diseases in plants. Bacillus subtilis strain EA-CB0015 produces iturin A, fengycin C and surfactin and it has been proven useful for the treatment of black Sigatoka disease in banana plants, an important pathology caused by the fungus Mycosphaerella fijiensis (Morelet). We have found that B. subtilis EA-CB0015 cell free supernatants and purified fractions inhibit M. fijiensis cellular growth...
May 10, 2017: Colloids and Surfaces. B, Biointerfaces
Jie Yue, Gang Fu, Dawei Zhang, Jianping Wen
OBJECTIVES: To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. RESULTS: An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region...
May 19, 2017: Biotechnology Letters
Taylor M Nye, Jeremy W Schroeder, Daniel B Kearns, Lyle A Simmons
Bacillus subtilis is a Gram-positive bacterium that serves as an important experimental system. B. subtilis NCIB 3610 is an undomesticated strain that exhibits phenotypes lost from the more common domesticated laboratory strains. Here, we announce the complete genome sequence of DK1042, a genetically competent derivative of NCIB 3610.
May 18, 2017: Genome Announcements
Smarajit Mondal, Alexander V Yakhnin, Paul Babitzke
The Bacillus subtilis trpEDCFBA operon is regulated by a transcription attenuation mechanism in which tryptophan-activated TRAP binds to the nascent transcript and blocks formation of an antiterminator structure such that formation of an overlapping intrinsic terminator causes termination in the 5' untranslated region (5' UTR). In the absence of bound TRAP the antiterminator forms and transcription continues into the trp genes. RNA polymerase pauses at positions U107 and U144 in the 5' UTR. The general transcription elongation factors NusA and NusG stimulate pausing at both positions...
May 15, 2017: Journal of Bacteriology
Anna F Wang Erickson, Padraig Deighan, Cinthia P Garcia, Robert O J Weinzierl, Ann Hochschild, Richard Losick
Sigma (σ) factors direct gene transcription by binding to and determining the promoter recognition specificity of RNA polymerase (RNAP) in bacteria. Genes transcribed under the control of alternative sigma factors allow cells to respond to stress and undergo developmental processes such as sporulation in Bacillus subtilis, in which gene expression is controlled by a cascade of alternative sigma factors. Binding of sigma factors to RNA polymerase depends on the coiled-coil (or clamp helices) motif of the β' subunit...
May 15, 2017: Journal of Bacteriology
Ujvala Deepthi Gadde, Sungtaek Oh, Youngsub Lee, Ellen Davis, Noah Zimmerman, Tom Rehberger, Hyun Soon Lillehoj
This study investigated the effects of Bacillus subtilis-based probiotics on the performance, modulation of host inflammatory responses and intestinal barrier gene expression of broilers subjected to LPS challenge. Chickens were randomly allocated to one of the 3 dietary treatment groups - control, antibiotic, or probiotic. At 14days, half of the chickens in each treatment were injected with LPS (1mg/kg body weight), and the other half injected with sterile PBS. Chickens fed probiotics weighed significantly more than controls at 15days of age, irrespective of immune challenge...
May 6, 2017: Research in Veterinary Science
Gumpanat Mahipant, Atchara Paemanee, Sittiruk Roytrakul, Junichi Kato, Alisa S Vangnai
BACKGROUND: Butanol is an intensively used industrial solvent and an attractive alternative biofuel, but the bioproduction suffers from its high toxicity. Among the native butanol producers and heterologous butanol-producing hosts, Bacillus subtilis 168 exhibited relatively higher butanol tolerance. Nevertheless, organic solvent tolerance mechanisms in Bacilli and Gram-positive bacteria have relatively less information. Thus, this study aimed to elucidate butanol stress responses that may involve in unique tolerance of B...
2017: Biotechnology for Biofuels
Zhanshuai Wu, Zeli Tang, Mei Shang, Lu Zhao, Lina Zhou, Xiangzhan Kong, Zhipeng Lin, Hengchang Sun, Tingjin Chen, Jin Xu, Xuerong Li, Yan Huang, Xinbing Yu
Clonorchiasis remains a nonnegligible public health problem in endemic areas. Cysteine protease of Clonorchis sinensis (CsCP) plays indispensable roles in the parasitic physiology and pathology, and has been exploited as a promising drug and vaccine candidate. In recent years, development of spore-based vaccines against multiple pathogens has attracted many investigators' interest. In previous studies, the recombinant Escherichia coli (BL21) and Bacillus subtilis spores expressing CsCP have been successfully constructed, respectively...
May 13, 2017: Parasitology Research
Jeff Errington, Ling Juan Wu
Bacillus subtilis is the best described member of the Gram positive bacteria. It is a typical rod shaped bacterium and grows by elongation in its long axis, before dividing at mid cell to generate two similar daughter cells. B. subtilis is a particularly interesting model for cell cycle studies because it also carries out a modified, asymmetrical division during endospore formation, which can be simply induced by starvation. Cell growth occurs strictly by elongation of the rod, which maintains a constant diameter at all growth rates...
2017: Sub-cellular Biochemistry
Mohaned Shilaih, Daniel C Angst, Alex Marzel, Sebastian Bonhoeffer, Huldrych F Günthard, Roger D Kouyos
BACKGROUND: Despite being used by more than 18 million people our understanding of the extent of effects of antiretrovirals on the human body and other organisms remains incomplete. In addition, the direct effect of antiretrovirals on the gut microbiota of HIV infected individuals has been largely overlooked in microbiome studies concerned with HIV infected individuals. METHODS: Here we tested 25 antiretrovirals on Bacillus subtilis and Escherichia coli using a broth microdilution assay to assess whether these drugs have an antibacterial effect...
May 12, 2017: Antiviral Therapy
Mahboobeh Talebi Mehrdar, Rasool Madani, Reza Hajihosseini, Soheila Moradi Bidhendi
The aim of this study is to investigate antibacterial effects of immunodominant proteins isolated from the venom of Naja Naja Oxiana snake against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa. The innate immune system is an important line of defense against bacterial diseases. Antibacterial peptides and proteins produced by snake venoms have recently attracted significant attention due to their relevance to bacterial diseases and the potential of being converted into new therapeutic agents...
2017: Iranian Journal of Pharmaceutical Research: IJPR
Zahoor Ahmad, Jia Wu, Lulu Chen, Wubei Dong
Plant growth-promoting bacteria (PGPB) may trigger tolerance against biotic/abiotic stresses and growth enhancement in plants. In this study, an endophytic bacterial strain from rapeseed was isolated to assess its role in enhancing plant growth and tolerance to abiotic stresses, as well as banded leaf and sheath blight disease in maize. Based on 16S rDNA and BIOLOG test analysis, the 330-2 strain was identified as Bacillus subtilis. The strain produced indole-3-acetic acid, siderophores, lytic enzymes and solubilized different sources of organic/inorganic phosphates and zinc...
May 11, 2017: Scientific Reports
Haofei Liu, Wenwen Xu, Qinghua Yu, Qian Yang
Deficient mucosal innate immunity is a hallmark of infectious diarrhea, such as Salmonella typhimurium (S. typhimurium)-induced gastroenteritis. Here, we report that oral administration of a 4,4'-diaponeurosporene-producing Bacillus subtilis (B.s-Dia) could improve mice mucosal immunity, as showed by an increased resistance against S. typhimurium infection. Intragastric administration of B.s-Dia for 7 days could increase the secretion of CCL20 by intestinal epithelial cells (IECs) and then recruit more dendritic cells...
2017: Frontiers in Immunology
Sang Il Gum, Phuc Anh Nguyen, Jong Rok Lee, Yeong Hwan Han, Min Kyung Cho
Red yeast rice product (RYP) has been used as a food supplement because of its lipid lowering, and in food additives as a natural colorant. Lovastatin of RYP is a hypolipidemic commercial drug. To enhance the beneficial effects of RYP, we performed a bioconversion with Bacillus subtilis. This B. subtilis-fermentation process of RYP increased the ratio of the active open-hydroxyl acid form and the prodrug lactone form of lovastatin, which is a potent cholesterol synthesis inhibitor. 3(2H)-benzofuranone was newly produced in the fermented red yeast rice product (FRYP) as analyzed by GC-MS...
October 1, 2017: Food Chemistry
Chun Gui, Shanwen Zhang, Xiangcheng Zhu, Wenjuan Ding, Hongbo Huang, Yu-Cheng Gu, Yanwen Duan, Jianhua Ju
Two new spirotetronate aglycones, 22-dehydroxymethyl-kijanolide (1) and 8-hydroxy-22-dehydroxymethyl-kijanolide (2), along with seven new spirotetronate glycosides, microsporanates A-F (3-8) and tetrocarcin P (9), together with three known tetrocarcins [tetrocarcins A (10), B (11), and AC6H (12)], were isolated from fermentation broths of the marine-derived Micromonospora harpali SCSIO GJ089. The structures of 1-9 were elucidated on the basis of 1D and 2D NMR and MS spectroscopic data. Compounds 3-8 feature an α,β-unsaturated carbonyl moiety within their spirotetronate skeletons...
May 10, 2017: Journal of Natural Products
Kashif Rasool, Khaled A Mahmoud, Daniel J Johnson, Mohamed Helal, Golibjon R Berdiyorov, Yury Gogotsi
Advanced membranes that enable ultrafast water flux while demonstrating anti-biofouling characteristics can facilitate sustainable water/wastewater treatment processes. MXenes, two-dimensional (2D) metal carbides and nitrides, have attracted attention for applications in water/wastewater treatment. In this work, we reported the antibacterial properties of micrometer-thick titanium carbide (Ti3C2Tx) MXene membranes prepared by filtration on a polyvinylidene fluoride (PVDF) support. The bactericidal properties of Ti3C2Tx modified membranes were tested against Escherichia coli (E...
May 9, 2017: Scientific Reports
Takashi Inaoka, Keitarou Kimura, Kazuya Morimatsu, Kazutaka Yamamoto
High hydrostatic pressure (HHP) affects various cellular processes. Using a sporulation-deficient Bacillus subtilis strain, we characterized the properties of vegetative cells subjected to HHP. When stationary-phase cells were exposed to 250 MPa of HHP for 10 min at 25 °C, approximately 50% of cells were viable, although they exhibited a prolonged growth lag. The HHP-injured cells autolyzed in the presence of NaCl or KCl (at concentrations ≥100 mM). Superoxide dismutase slightly protected the viability of HHP-treated cells, whereas vegetative catalases had no effect...
June 2017: Bioscience, Biotechnology, and Biochemistry
Qianqian Wang, Yixiang Xu, Jiaqi Xu, Xudong Wang, Chen Shen, Yan Zhang, Xiufeng Liu, Boyang Yu, Jian Zhang
OBJECTIVES: To characterize glycosyltransferases from Bacillus subtilis ATCC 6633 and investigate their substrate specificity towards plant polyphenols. RESULTS: Among the cloned and expressed six UDP-glycosyltransferases (BsGT1-6), BsGT-1 showed activity with a wide range of polyphenols: morin, quercetin, alizarin, rehin, curcumin and aloe emodin. The gene of BsGT-1 has an ORF of 1206 bp encoding 402 amino acids. The recombinant enzyme was purified to homogeneity by Ni-NTA affinity chromatograph, and its biochemical characteristics were identified by HPLC-UV/MS, (1)H-NMR and (13)C-NMR...
May 8, 2017: Biotechnology Letters
Weiguang Kong, Can Huang, Ying Tang, Ding Zhang, Zhixin Wu, Xiaoxuan Chen
Our study explored the effect of oral intubation of Bacillus subtilis on Aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp. The mid-intestine mucosal tissue was collected for ATPase activity measurement. Intestinal mucosa was also ultrastructurally examined with transmission electron microscope (TEM), and its permeability was determined using Evans blue (EB) and D-lactic acid. The mid-intestine pro-inflammation cytokine, MyD88 and tight junction (TJ) protein mRNA expression levels were measured using real-time quantitative PCR...
May 8, 2017: Scientific Reports
Cierra Birch, Madison Davis, Lea Mbengi, Peter Zuber
Bacillus subtilis Spx is a global transcriptional regulator that is conserved among gram-positive bacteria, in which Spx is required for preventing oxidatively induced proteotoxicity. Upon stress induction, Spx engages RNA polymerase (RNAP) through interaction with the C-terminal domain of the rpoA-encoded RNAP α subunit (αCTD). Previous mutational analysis of rpoA revealed that substitutions of Y263 in αCTD severely impaired Spx-activated transcription. Attempts to substitute alanine for αCTD R261, R268, R289, E255, E298 and K294 were unsuccessful, suggesting that these residues are essential...
May 8, 2017: Journal of Bacteriology
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