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B. subtilis

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https://www.readbyqxmd.com/read/28231455/synthetic-investigation-physicochemical-characterization-and-antibacterial-evaluation-of-ternary-bi-iii-systems-with-hydroxycarboxylic-acid-and-aromatic-chelator-substrates
#1
C M Nday, E Halevas, A Tsiaprazi-Stamou, D Eleftheriadou, A Hatzidimitriou, G Jackson, D Reid, A Salifoglou
Due to its physical and chemical properties, bismuth (Bi(III)) is widely used in the treatment of several gastrointestinal and skin diseases, and infections caused by bacteria. Herein, its known antimicrobial potential was taken into consideration in the synthesis of two new hybrid ternary materials of Bi(III) with the physiological α-hydroxycarboxylic glycolic acid and 1,10-phenanthroline (phen), [Bi2(C2H2O3)2(C2H3O3)(NO3)]n(.) nH2O (1) and [Bi(C12H8N2)(NO3)4](C10H8N4) (2), aiming at improving its antibacterial properties...
February 11, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28226280/development-of-electrochemical-biosensor-for-detection-of-pathogenic-microorganism-in-asian-dust-events
#2
Min-Sang Yoo, Minguk Shin, Younghun Kim, Min Jang, Yoon-E Choi, Si Jae Park, Jonghoon Choi, Jinyoung Lee, Chulhwan Park
We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor...
February 19, 2017: Chemosphere
https://www.readbyqxmd.com/read/28223976/plant-growth-promotion-by-volatile-organic-compounds-produced-by-bacillus-subtilis-syst2
#3
Hafiz A S Tahir, Qin Gu, Huijun Wu, Waseem Raza, Alwina Hanif, Liming Wu, Massawe V Colman, Xuewen Gao
Bacterial volatiles play a significant role in promoting plant growth by regulating the synthesis or metabolism of phytohormones. In vitro and growth chamber experiments were conducted to investigate the effect of volatile organic compounds (VOCs) produced by the plant growth promoting rhizobacterium Bacillus subtilis strain SYST2 on hormone regulation and growth promotion in tomato plants. We observed a significant increase in plant biomass under both experimental conditions; we observed an increase in photosynthesis and in the endogenous contents of gibberellin, auxin, and cytokinin, while a decrease in ethylene levels was noted...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28223783/preparation-and-biosorption-evaluation-of-bacillus-subtilis-alginate-chitosan-microcapsule
#4
Ke Tong
The aim of this study was to assess the effect of alginate-chitosan microcapsule on viability characteristics of Bacillus subtilis and the ability of B. subtilis/alginate-chitosan microcapsule to remove uranium ion from aqueous solution. The effects of particle size, chitosan molecular weight and inoculum density on viability characteristics were studied using alginate-chitosan microcapsule-immobilized B. subtilis experiments. In addition, the effects of pH, immobilized spherule dosage, temperature, initial uranium ion concentration and contact time on removal of uranium ion were studied using batch adsorption experiments...
2017: Nanotechnology, Science and Applications
https://www.readbyqxmd.com/read/28221892/effects-of-ohmic-heating-including-electric-field-intensity-and-frequency-on-thermal-inactivation-of-bacillus-subtilis-spores
#5
Suguru Murashita, Shuso Kawamura, Shigenobu Koseki
Methods for microbial inactivation are important in the food industry; however, conventional external heating (CH) reduces food quality. Accordingly, the nonthermal effects of ohmic heating (OH) on Bacillus subtilis spores in a sodium chloride aqueous solution at 101°C (i.e., the boiling point), as well as the effects of electric field intensity and frequency during OH, were investigated. Survival kinetics were compared between OH and external CH. The inactivation effect on B. subtilis was greater for all electric field conditions (5, 10, and 20 V/cm) than for CH...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221889/inactivation-of-gram-positive-bacteria-by-novel-phenolic-branched-chain-fatty-acids
#6
Xuetong Fan, Karen Wagner, Kimberly J B Sokorai, Helen Ngo
Novel phenolic branched-chain fatty acids (PBC-FAs) were evaluated for their antimicrobial properties against both gram-positive ( Listeria innocua , Bacillus subtilis , Enterococcus faecium ) and gram-negative ( Escherichia coli , Salmonella Typhimurium, and Pseudomonas tolaasii ) bacteria. In addition, PBC-FA derivatives, such as PBC-FA methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-PBC-FA methyl esters, were synthesized to study the structure activity relationship. Results showed that PBC-FAs were a potent antimicrobial against gram-positive bacteria with MICs of 1...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28219382/high-level-extracellular-protein-production-in-bacillus-subtilis-using-an-optimized-dual-promoter-expression-system
#7
Kang Zhang, Lingqia Su, Xuguo Duan, Lina Liu, Jing Wu
BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, PamyQ from Bacillus amyloliquefaciens, should improve protein expression using this strain. This study was undertaken to develop a new, high-level expression system in B...
February 20, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28212310/presence-of-calcium-lowers-the-expansion-of-bacillus-subtilis-colony-biofilms
#8
Eisha Mhatre, Anandaroopan Sundaram, Theresa Hölscher, Mike Mühlstädt, Jörg Bossert, Ákos T Kovács
Robust colony formation by Bacillus subtilis is recognized as one of the sessile, multicellular lifestyles of this bacterium. Numerous pathways and genes are responsible for the architecturally complex colony structure development. Cells in the biofilm colony secrete extracellular polysaccharides (EPS) and protein components (TasA and the hydrophobin BslA) that hold them together and provide a protective hydrophobic shield. Cells also secrete surfactin with antimicrobial as well as surface tension reducing properties that aid cells to colonize the solid surface...
February 16, 2017: Microorganisms
https://www.readbyqxmd.com/read/28209633/gene-expression-control-by-bacillus-anthracis-purine-riboswitches
#9
Marion Kirchner, Sabine Schneider
In all kingdoms of life, cellular replication relies on the presence of nucleosides and nucleotides, the building blocks of nucleic acids and the main source of energy. In bacteria, the availability of metabolites sometimes directly regulates the expression of enzymes and proteins involved in purine salvage, biosynthesis and uptake through riboswitches. Riboswitches are located in bacterial mRNAs and can control gene expression by conformational changes in response to ligand binding. We have established an inverse reporter gene system in Bacillus subtilis that allows us to monitor riboswitch-controlled gene expression...
February 16, 2017: RNA
https://www.readbyqxmd.com/read/28204884/selection-of-bacillus-species-for-targeted-in-situ-release-of-prebiotic-galacto-rhamnogalacturonan-from-potato-pulp-in-piglets
#10
Carsten Jers, Mikael L Strube, Mette D Cantor, Bea K K Nielsen, Ole B Sørensen, Mette Boye, Anne S Meyer
We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase...
February 15, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28202619/exopolysaccharide-from-bacillus-subtilis-induces-anti-inflammatory-m2-macrophages-that-prevent-t-cell-mediated-disease
#11
Mallory L Paynich, Sara E Jones-Burrage, Katherine L Knight
Commensal bacteria contribute to immune homeostasis in the gastrointestinal tract; however, the underlying mechanisms for this are not well understood. A single dose of exopolysaccharide (EPS) from the probiotic spore-forming bacterium Bacillus subtilis protects mice from acute colitis induced by the enteric pathogen Citrobacter rodentium Adoptive transfer of macrophage-rich peritoneal cells from EPS-treated mice confers protection from disease to recipient mice. In vivo, EPS induces development of anti-inflammatory M2 macrophages in a TLR4-dependent manner, and these cells inhibit T cell activation in vitro and in C...
February 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28196701/filamenting-temperature-sensitive-mutant-z-inhibitors-from-glycyrrhiza-glabra-and-their-inhibitory-mode-of-action
#12
Takashi Matsui, Subehan Lallo, Khoirun Nisa, Hiroyuki Morita
FtsZ is an essential protein for bacterial cell division, and an attractive and underexploited novel antibacterial target protein. Screening of Indonesian plants revealed the inhibitory activity of the methanol extract of Glycyrrhiza glabra on the Bacillus subtilis FtsZ (BsFtsZ) GTPase, and further bioassay-guided fractionation of the active methanol extract led to the isolation of seven known polyketides (1-7). Among them, gancaonin I (1), glycyrin (3), and isolicoflavanol (5) exhibited anti-BsFtsZ GTPase activities, at levels comparable to that of the synthetic FtsZ inhibitor, Zantrin Z3...
February 3, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28196402/pimelic-acid-the-first-precursor-of-the-bacillus-subtilis-biotin-synthesis-pathway-exists-as-the-free-acid-and-is-assembled-by-fatty-acid-synthesis
#13
Miglena Manandhar, John E Cronan
Biotin synthetic pathways are readily separated into two stages, synthesis of the seven carbon α, ω-dicarboxylic acid pimelate moiety and assembly of the fused heterocyclic rings. The biotin pathway genes responsible for pimelate moiety synthesis vary widely among bacteria whereas the ring synthesis genes are highly conserved. Bacillus subtilis seems to have redundant genes, bioI and bioW, for generation of the pimelate intermediate. Largely consistent with previous genetic studies we found that deletion of bioW caused a biotin auxotrophic phenotype whereas deletion of bioI did not...
February 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28193635/a-redox-responsive-transcription-factor-is-critical-for-pathogenesis-and-aerobic-growth-of-listeria-monocytogenes
#14
Aaron T Whiteley, Brittany R Ruhland, Mauna B Edrozo, Michelle L Reniere
Bacterial pathogens have evolved sophisticated mechanisms to sense and adapt to redox stress in nature and within the host. However, deciphering the redox environment encountered by intracellular pathogens in the mammalian cytosol is challenging and remains poorly understood. In this study, we assessed the contributions of the two redox-responsive, Spx-family transcriptional regulators to the virulence of Listeria monocytogenes, a Gram-positive facultative intracellular pathogen. Spx-family proteins are highly conserved in Firmicutes and L...
February 13, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28193334/glutamate-dehydrogenase-rocg-in-bacillus-licheniformis-wx-02-enzymatic-properties-and-specific-functions-in-glutamic-acid-synthesis-for-poly-%C3%AE-glutamic-acid-production
#15
Guangming Tian, Qin Wang, Xuetuan Wei, Xin Ma, Shouwen Chen
Poly-γ-glutamic acid (γ-PGA), a natural biopolymer, is widely used in cosmetics, medicine, food, water treatment, and agriculture owing to its features of moisture sequestration, cation chelation, non-toxicity and biodegradability. Intracellular glutamic acid, the substrate of γ-PGA, is a limiting factor for high yield in γ-PGA production. Bacillus subtilis and Bacillus licheniformis are both important γ-PGA producing strains, and B. subtilis synthesizes glutamic acid in vivo using the unique GOGAT/GS pathway...
April 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28191448/a-food-grade-expression-system-for-d-psicose-3-epimerase-production-in-bacillus-subtilis-using-an-alanine-racemase-encoding-selection-marker
#16
Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun, Dawei Zhang
BACKGROUND: Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expression system for d-psicose 3-epimerase production, we use an alanine racemase-encoding gene as selection marker in Bacillus subtilis. RESULTS: In this study, the d-alanine racemase-encoding gene dal was deleted from the chromosome of B. subtilis 1A751 using Cre/lox system to generate the food-grade host...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28189097/thermo-responsive-nanoarrays-of-silver-nanoparticle-silicate-nanoplatelet-and-pnipaam-for-the-antimicrobial-applications
#17
Hsiao-Chu Lin, Yu-An Su, Ting-Yu Liu, Yu-Jane Sheng, Jiang-Jen Lin
The ternary nanohybrids of silver nanoparticles (AgNPs) in combination with silicate nanoplatelets (NSP) and thermally sensitive poly(N-isopropylacrylamide) (PNiPAAm) were fabricated for antibacterial applications. PNiPAAm were chemically grafted on the NSP by atom-transfer radical polymerization (ATRP) via polymerizing N-isopropylacrylamide monomers with sol-gel linkers (BBTES). The nanoparticles of AgNPs then were adsorbed on NSP-PNiPAAm nanosheets through in situ reduction reaction of AgNO3 in aqueous dispersion...
January 27, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28183607/anti-microbial-surfaces-an-approach-for-deposition-of-zno-nanoparticles-on-pva-gelatin-composite-film-by-screen-printing-technique
#18
J V Meshram, V B Koli, M R Phadatare, S H Pawar
Initially micro-organisms get exposed to the surfaces, this demands development of anti-microbial surfaces to inhibit their proliferation. Therefore, herein, we attempt screen printing technique for development of PVA-GE/ZnO nanocomposite (PG/ZnO) films. The synthesis of PG/ZnO nanocomposite includes two steps as: (i) Coating of Zinc Oxide nanoparticles (ZnO NPs) by poly ethylene glycol in order to be compatible with organic counterparts. (ii) Deposition of coated nanoparticles on the PG film surface. The results suggest the enhancement in anti-microbial activity of PG/ZnO nanocomposite over pure ZnO NPs against both Gram positive Bacillus subtilis and Gram negative Escherichia coli from zone of inhibition...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28174308/bacillus-subtilis-swarmer-cells-lead-the-swarm-multiply-and-generate-a-trail-of-quiescent-descendants
#19
Lina Hamouche, Soumaya Laalami, Adrian Daerr, Solène Song, I Barry Holland, Simone J Séror, Kassem Hamze, Harald Putzer
: Bacteria adopt social behavior to expand into new territory, led by specialized swarmers, before forming a biofilm. Such mass migration of Bacillus subtilis on a synthetic medium produces hyperbranching dendrites that transiently (equivalent to 4 to 5 generations of growth) maintain a cellular monolayer over long distances, greatly facilitating single-cell gene expression analysis. Paradoxically, while cells in the dendrites (nonswarmers) might be expected to grow exponentially, the rate of swarm expansion is constant, suggesting that some cells are not multiplying...
February 7, 2017: MBio
https://www.readbyqxmd.com/read/28168443/crystal-structure-and-biochemical-characterization-of-the-transmembrane-pap2-type-phosphatidylglycerol-phosphate-phosphatase-from-bacillus-subtilis
#20
Meriem El Ghachi, Nicole Howe, Rodolphe Auger, Alexandre Lambion, Annick Guiseppi, François Delbrassine, Guillaume Manat, Sophie Roure, Sabine Peslier, Eric Sauvage, Lutz Vogeley, Juan-Carlos Rengifo-Gonzalez, Paulette Charlier, Dominique Mengin-Lecreulx, Maryline Foglino, Thierry Touzé, Martin Caffrey, Frédéric Kerff
Type 2 phosphatidic acid phosphatases (PAP2s) can be either soluble or integral membrane enzymes. In bacteria, integral membrane PAP2s play major roles in the metabolisms of glycerophospholipids, undecaprenyl-phosphate (C55-P) lipid carrier and lipopolysaccharides. By in vivo functional experiments and biochemical characterization we show that the membrane PAP2 coded by the Bacillus subtilis yodM gene is the principal phosphatidylglycerol phosphate (PGP) phosphatase of B. subtilis. We also confirm that this enzyme, renamed bsPgpB, has a weaker activity on C55-PP...
February 6, 2017: Cellular and Molecular Life Sciences: CMLS
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