Hazel Wilkie, Mrinmoy Das, Tyler Pelovitz, Wayne Bainter, Brian Woods, Mohammed Alasharee, Ali Sobh, Safa Baris, Sevgi Bilgic Eltan, Waleed Al-Herz, Mohamed-Ridha Barbouche, Imen Ben-Mustapha, Meriem Ben-Ali, Mohamed T H Sallam, Amany Awad, Sohilla Lotfy, Aisha El Marsafy, Moushira Ezzelarab, Michael Farrar, Brigitta A R Schmidt, Monali NandyMazumdar, Emma Guttman-Yassky, Anthony Sheets, Katie Maria Vidic, George Murphy, Patrick M Schlievert, Janet Chou, Juan Manuel Leyva-Castillo, Erin Janssen, Maheshwor Timilshina, Raif S Geha
BACKGROUND: DOCK8-deficient patients have severe eczema, elevated IgE and eosinophilia, features of Atopic Dermatitis (AD). OBJECTIVE: To understand the mechanisms of eczema in DOCK8 deficiency. METHODS: Skin biopsies were characterized for histology, immuno-fluorescence microscopy, and gene expression. Skin barrier function was measured by trans-epidermal water loss. Allergic skin inflammation was elicited in mice by epicutaneous (EC) sensitization with ovalbumin (OVA) or cutaneous application of S...
January 5, 2024: Journal of Allergy and Clinical Immunology