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Daniela Damasceno, Martín Pérez Andrés, Wouter Bl van den Bossche, Juan Flores-Montero, Sandra de Bruin, Cristina Teodosio, Jacques Jm van Dongen, Alberto Orfao, Julia Almeida
Although major steps have been recently made in understanding the role of the distinct subsets of dendritic cells (DC)/antigen-presenting cells (APC), further studies are required to unravel their precise role, including in-depth immunophenotypic characterisation of these cells. Here, we used eight-colour flow cytometry to investigate the reactivity of a panel of 72 monoclonal antibodies (including those clustered in seven new Cluster of Differentiation, CD) on different subsets of APC in peripheral blood (PB) samples from five healthy adults...
September 2016: Clinical & Translational Immunology
Theresia M Westers, Eline M P Cremers, Uta Oelschlaegel, Ulrika Johansson, Peter Bettelheim, Sergio Matarraz, Alberto Orfao, Bijan Moshaver, Lisa Eidenschink Brodersen, Michael R Loken, Denise A Wells, Dolores Subira', Matthew Cullen, Jeroen G Te Marvelde, Vincent H J Van der Velden, Frank W M B Preijers, Sung-Chao Chu, Jean Feuillard, Estelle Guérin, Katherina Psarra, Anna Porwit, Leonie Saft, Robin Ireland, Timothy Milne, Marie C Béné, Birgit I Witte, Matteo G Della Porta, Wolfgang Kern, Arjan A van de Loosdrecht
Current recommendations for diagnosing myelodysplastic syndromes endorse flow cytometry as an informative tool. Most flow cytometry protocols focus on the analysis of progenitor cells and the evaluation of the maturing myelomonocytic lineage. However, one of the most frequently observed features of myelodysplastic syndromes is anemia, which may be associated with dyserythropoiesis. Therefore, analysis of changes in flow cytometry features of nucleated erythroid cells may complement current flow cytometry tools...
October 6, 2016: Haematologica
Sara Alonso-Álvarez, Maria Belén Vidriales, Maria Dolores Caballero, Oscar Blanco, Noemí Puig, Alejandro Martin, Maria Jesús Peñarrubia, Esther Zato, Josefina Galende, Abelardo Bárez, Miguel Alcoceba, Alberto Orfão, Marcos González, Ramón García-Sanz
Prognostic factors in Hodgkin lymphoma (HL) still fail to accurately identify high-risk patients. Tumor microenvironment in HL is a current focus of research for risk definition but few studies have focused on infiltrating lymphocytes. Here, we analyzed the number of tumor infiltrating lymphocytes by flow cytometry in diagnostic biopsies from 96 HL homogeneously treated patients with ABVD with or without radiotherapy. Most lymph node cells were lymphocytes (90 ± 17), with a median T/B/NK distribution of 74%/26%/0...
October 12, 2016: Leukemia & Lymphoma
Alvaro Otero-Rodriguez, Maria Dolores Tabernero, Maria Cristina Munoz-Martin, Pablo Sousa, Alberto Orfao, Daniel Pascual-Argente, Maria Gonzalez-Tablas, Laura Ruiz-Martin
BACKGROUND: Since 1957, Simpson grading system is considered a predictive system for meningiomas recurrences. However, since then, there has been a great development of surgical equipment and neurosurgical technique, so the re-evaluation of this grading system should be considered. This study aims to assess if the recurrence rates and recurrence-free survivals (RFS) are different after Simpson grades I, II, and III resections in World Health Organization (WHO) grade I meningiomas METHOD: We retrospectively reviewed the data of patients who underwent surgical treatment of WHO grade I meningiomas located in convexity (group 1), falx/parasagittal (group 2), skull base, and tentorium (group 3) between June 1991 and December 2011...
September 13, 2016: World Neurosurgery
Marta Morado, Alex Freire Sandes, Enrique Colado, Dolores Subirá, Paloma Isusi, María Soledad Noya, María Belén Vidriales, Amparo Sempere, José Ángel Díaz, Alfredo Minguela, Beatriz Álvarez, Cristina Serrano, Teresa Caballero, Mercedes Rey, Ana Pérez Corral, María Cristina Fernández Jiménez, Elena Magro, Angelina Lemes, Celina Benavente, Helena Bañas, Juana Merino, Celine Castejon, Olivier Gutierrez, Pilar Rabasa, Matheus Vescosi Gonçalves, Martin Perez-Andres, Alberto Orfao
BACKGROUND: Although consensus guidelines have been proposed in 2010 for the diagnostic screening of paroxysmal nocturnal hemoglobinuria (PNH) by flow cytometry (FCM), so far no study has investigated the efficiency of such medical indications in multicentric vs. reference laboratory settings. METHODS: Here we evaluate the efficiency of consensus medical indications for PNH testing in 3,938 peripheral blood samples submitted to FCM testing in 24 laboratories in Spain and one reference center in Brazil...
September 6, 2016: Cytometry. Part B, Clinical Cytometry
Shaji Kumar, Bruno Paiva, Kenneth C Anderson, Brian Durie, Ola Landgren, Philippe Moreau, Nikhil Munshi, Sagar Lonial, Joan Bladé, Maria-Victoria Mateos, Meletios Dimopoulos, Efstathios Kastritis, Mario Boccadoro, Robert Orlowski, Hartmut Goldschmidt, Andrew Spencer, Jian Hou, Wee Joo Chng, Saad Z Usmani, Elena Zamagni, Kazuyuki Shimizu, Sundar Jagannath, Hans E Johnsen, Evangelos Terpos, Anthony Reiman, Robert A Kyle, Pieter Sonneveld, Paul G Richardson, Philip McCarthy, Heinz Ludwig, Wenming Chen, Michele Cavo, Jean-Luc Harousseau, Suzanne Lentzsch, Jens Hillengass, Antonio Palumbo, Alberto Orfao, S Vincent Rajkumar, Jesus San Miguel, Herve Avet-Loiseau
Treatment of multiple myeloma has substantially changed over the past decade with the introduction of several classes of new effective drugs that have greatly improved the rates and depth of response. Response criteria in multiple myeloma were developed to use serum and urine assessment of monoclonal proteins and bone marrow assessment (which is relatively insensitive). Given the high rates of complete response seen in patients with multiple myeloma with new treatment approaches, new response categories need to be defined that can identify responses that are deeper than those conventionally defined as complete response...
August 2016: Lancet Oncology
B Paiva, N Puig, M T Cedena, B G de Jong, Y Ruiz, I Rapado, J Martinez-Lopez, L Cordon, D Alignani, J A Delgado, M C van Zelm, J J M Van Dongen, M Pascual, X Agirre, F Prosper, J I Martín-Subero, M-B Vidriales, N C Gutierrez, M T Hernandez, A Oriol, M A Echeveste, Y Gonzalez, S K Johnson, J Epstein, B Barlogie, G J Morgan, A Orfao, J Blade, M V Mateos, J J Lahuerta, J F San-Miguel
The notion that plasma cells (PCs) are terminally differentiated has prevented intensive research in multiple myeloma (MM) about their phenotypic plasticity and differentiation. Here, we demonstrated in healthy individuals (n=20) that the CD19-CD81 expression axis identifies three bone marrow (BM)PC subsets with distinct age-prevalence, proliferation, replication-history, immunoglobulin-production, and phenotype, consistent with progressively increased differentiation from CD19+CD81+ into CD19-CD81+ and CD19-CD81- BMPCs...
September 16, 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Iván Alvarez-Twose, Almudena Matito, José Mário Morgado, Laura Sánchez-Muñoz, María Jara-Acevedo, Andrés García-Montero, Andrea Mayado, Carolina Caldas, Cristina Teodósio, Javier Ignacio Muñoz-González, Manuela Mollejo, Luis Escribano, Alberto Orfao
Resistance to imatinib has been recurrently reported in systemic mastocytosis (SM) carrying exon 17 KIT mutations. We evaluated the efficacy and safety of imatinib therapy in 10 adult SM patients lacking exon 17 KIT mutations, 9 of which fulfilled criteria for well-differentiated SM (WDSM). The World Health Organization 2008 disease categories among WDSM patients were mast cell (MC) leukemia (n = 3), indolent SM (n = 3) and cutaneous mastocytosis (n = 3); the remainder case had SM associated with a clonal haematological non-MC disease...
July 19, 2016: Oncotarget
Sónia A C Carabineiro, Vera P Santos, M Fernando R Pereira, José J M Órfão, José L Figueiredo
Cryptomelane-type manganese oxides were synthesized by redox reaction under acid and reflux conditions. Different metals (cesium, lithium and titanium) were incorporated into the tunnel structure by the ion-exchange technique. Gold was loaded onto these materials (1wt%) by a double impregnation method. The obtained catalysts were characterized by high-resolution transmission electron microscopy, energy-dispersive X-ray spectrometry, scanning electron microscopy, X-ray diffraction and temperature-programmed reduction...
October 15, 2016: Journal of Colloid and Interface Science
M M Sales, Siacp Ferreira, Mrv Ikoma, A F Sandes, M P Beltrame, N S Bacal, McA Silva, M Malvezzi, Igh Lorand-Metze, A Orfao, M Yamamoto
BACKGROUND: Multiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful for the classification of chronic lymphoproliferative disorders (CLPD) according to the WHO criteria. Following the purposes of the Brazilian Group of Flow Cytometry (GBCFLUX), the aim of this report was to standardize the minimum requirements to achieve an accurate diagnosis in CLPDs, considering the different economic possibilities of the laboratories in our country...
June 30, 2016: Cytometry. Part B, Clinical Cytometry
Ronaldo Barros Orfão, Jessica Alves, Fernando Heering Bartoloni
A fluorescent imidazolyl-phenolic compound was applied on the detection of metallic species (Cu(2+), Al(3+), Cr(3+) and Fe(3+)) in a CH3CN/H2O (95/5, v/v) media. The presence and concentration of these cations altered significantly the emission profile of the probe, mainly lowering the signal intensity at 466 nm, while a new emission band around 395 nm appeared (for the trivalent ions). These results were rationalized as a combination of collisional quenching (KSV in the 10(3)-10(4) L mol(-1) range) and formation of a coordinated compound...
July 2016: Journal of Fluorescence
Sónia Alexandra Correia Carabineiro, Michalis Konsolakis, George Emmanouil-Nontas Marnellos, Muhammad Faizan Asad, Olívia Salomé Gonçalves Pinto Soares, Pedro Bandeira Tavares, Manuel Fernando Ribeiro Pereira, José Joaquim de Melo Órfão, José Luís Figueiredo
Different lanthanide (Ln)-doped cerium oxides (Ce0.5Ln0.5O1.75, where Ln: Gd, La, Pr, Nd, Sm) were loaded with Cu (20 wt. %) and used as catalysts for the oxidation of ethyl acetate (EtOAc), a common volatile organic compound (VOC). For comparison, both Cu-free (Ce-Ln) and supported Cu (Cu/Ce-Ln) samples were characterized by N₂ adsorption at -196 °C, scanning/transmission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy and temperature programmed reduction in H₂...
2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Bruno Paiva, Joaquin Martinez-Lopez, Luis A Corchete, Beatriz Sanchez-Vega, Inmaculada Rapado, Noemi Puig, Santiago Barrio, Maria-Luz Sanchez, Diego Alignani, Marta Lasa, Alfonso García de Coca, Emilia Pardal, Alberto Oriol, Maria-Esther Gonzalez Garcia, Fernando Escalante, Tomás J González-López, Luis Palomera, José Alonso, Felipe Prosper, Alberto Orfao, Maria-Belen Vidriales, María-Victoria Mateos, Juan-Jose Lahuerta, Norma C Gutierrez, Jesús F San Miguel
Immunoglobulin light-chain amyloidosis (AL) and multiple myeloma (MM) are 2 distinct monoclonal gammopathies that involve the same cellular compartment: clonal plasma cells (PCs). Despite the fact that knowledge about MM PC biology has significantly increased in the last decade, the same does not apply for AL. Here, we used an integrative phenotypic, molecular, and genomic approach to study clonal PCs from 24 newly diagnosed patients with AL. Through principal-component-analysis, we demonstrated highly overlapping phenotypic profiles between AL and both monoclonal gammopathy of undetermined significance and MM PCs...
June 16, 2016: Blood
Elen Oliveira, Thiago S Bacelar, Juana Ciudad, Maria Cecília M Ribeiro, Daniela R N Garcia, Lukasz Sedek, Simone F Maia, Daniel B Aranha, Indyara C Machado, Arissa Ikeda, Bianca F Baglioli, Nathalia Lopez-Duarte, Lisandra A C Teixeira, Tomasz Szczepanski, Maria Luiza M Silva, Marcelo G P Land, Alberto Orfao, Elaine S Costa
An increasing number of evidences suggest a genetic predisposition in acute lymphoblastic leukemia (ALL) that might favor the occurrence of the driver genetic alterations. Such genetic background might also translate into phenotypic alterations of residual hematopoietic cells. Whether such phenotypic alterations are present in bone marrow (BM) cells from childhood B-cell precursor (BCP)-ALL remains to be investigated. Here we analyzed the immunophenotypic profile of BM and peripheral blood (PB) maturing/matured neutrophils from 118 children with BCP-ALL and their relationship with the features of the disease...
April 26, 2016: Oncotarget
Paula Díez, Seila Lorenzo, Rosa M Dégano, Nieves Ibarrola, María González-González, Wendy Nieto, Julia Almeida, Marcos González, Alberto Orfao, Manuel Fuentes
Chronic lymphocytic leukaemia (CLL) is a malignant B cell disorder characterized by its high heterogeneity. Although genomic alterations have been broadly reported, protein studies are still in their early stages. Herein, a 224-antibody microarray has been employed to study the intracellular signalling pathways in a cohort of 14 newly diagnosed B-CLL patients as a preliminary study for further investigations. Several protein profiles were differentially identified across the cytogenetic and molecular alterations presented in the samples (deletion 13q14 and 17p13...
April 2016: Proteomics
I Alvarez-Twose, P Martínez-Barranco, J Gotlib, A García-Montero, J M Morgado, M Jara-Acevedo, J D Merker, F J Peñalver, A Matito, Y Hou, L Sánchez-Muñoz, A Mayado, M Mollejo, L Escribano, A Orfao
No abstract text is available yet for this article.
August 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Lourdes Martín-Martín, Julia Almeida, Helena Pomares, Eva González-Barca, Pilar Bravo, Teresa Giménez, Cecilia Heras, José-Antonio Queizán, Elena Pérez-Ceballos, Violeta Martínez, Natalia Alonso, Carlota Calvo, Rodolfo Álvarez, María Dolores Caballero, Alberto Orfao
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare aggressive myeloid neoplasm which shows a high rate of central nervous system (CNS) recurrence and overall survival (OS) of <1 year. Despite this, screening for CNS involvement is not routinely performed at diagnosis and intrathecal (IT) prophylaxis is not regularly administered in BPDCN. Here, we prospectively evaluated 13 consecutive BPDCN patients for the presence of CNS involvement by flow cytometry. Despite none of the patients presented with neurological symptoms, occult CNS involvement was detected in 6/10 cases evaluated at diagnosis and 3/3 studied at relapse/progression...
March 1, 2016: Oncotarget
Andres C Garcia-Montero, Maria Jara-Acevedo, Ivan Alvarez-Twose, Cristina Teodosio, Laura Sanchez-Muñoz, Carmen Muñiz, Javier I Muñoz-Gonzalez, Andrea Mayado, Almudena Matito, Carolina Caldas, Jose M Morgado, Luis Escribano, Alberto Orfao
Multilineage involvement of bone marrow (BM) hematopoiesis by the somatic KIT D816V mutation is present in a subset of adult indolent systemic mastocytosis (ISM) patients in association with a poorer prognosis. Here, we investigated the potential involvement of BM mesenchymal stem cells (MSCs) from ISM patients by the KIT D816V mutation and its potential impact on disease progression and outcome. This mutation was investigated in highly purified BM MSCs and other BM cell populations from 83 ISM patients followed for a median of 116 months...
February 11, 2016: Blood
María-Belén Vidriales, Estefanía Pérez-López, Carlota Pegenaute, Marta Castellanos, José-Juan Pérez, Mauricio Chandía, Joaquín Díaz-Mediavilla, Consuelo Rayón, Natalia de Las Heras, Pascual Fernández-Abellán, Miguel Cabezudo, Alfonso García de Coca, Jose M Alonso, Carmen Olivier, Jesús M Hernández-Rivas, Pau Montesinos, Rosa Fernández, Julio García-Suárez, Magdalena García, María-José Sayas, Bruno Paiva, Marcos González, Alberto Orfao, Jesús F San Miguel
The clinical utility of minimal residual disease (MRD) analysis in acute myeloid leukaemia (AML) is not yet defined. We analysed the prognostic impact of MRD level at complete remision after induction therapy using multiparameter flow cytometry in 306 non-APL AML patients. First, we validated the prognostic value of MRD-thresholds we have previously proposed (≥ 0.1%; ≥ 0.01-0.1%; and <0.01), with a 5-year RFS of 38%, 50% and 71%, respectively (p=0.002). Cytogenetics is the most relevant prognosis factor in AML, however intermediate risk cytogenetics represent a grey zone that require other biomarkers for risk stratification, and we show that MRD evaluation discriminate three prognostic subgroups (p=0...
January 2016: Leukemia Research
Paloma Bárcena, María Jara-Acevedo, María Dolores Tabernero, Antonio López, María Luz Sánchez, Andrés C García-Montero, Noemí Muñoz-García, María Belén Vidriales, Artur Paiva, Quentin Lecrevisse, Margarida Lima, Anton W Langerak, Sebastian Böttcher, Jacques J M van Dongen, Alberto Orfao, Julia Almeida
Currently, the lack of a universal and specific marker of clonality hampers the diagnosis and classification of chronic expansions of natural killer (NK) cells. Here we investigated the utility of flow cytometric detection of aberrant/altered NK-cell phenotypes as a surrogate marker for clonality, in the diagnostic work-up of chronic lymphoproliferative disorders of NK cells (CLPD-NK). For this purpose, a large panel of markers was evaluated by multiparametric flow cytometry on peripheral blood (PB) CD56(low) NK cells from 60 patients, including 23 subjects with predefined clonal (n = 9) and polyclonal (n = 14) CD56(low) NK-cell expansions, and 37 with CLPD-NK of undetermined clonality; also, PB samples from 10 healthy adults were included...
December 15, 2015: Oncotarget
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