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https://www.readbyqxmd.com/read/28230980/chitosan-modified-filter-paper-for-nucleic-acid-extraction-and-in-situ-pcr-on-a-thermoplastic-microchip
#1
Wupeng Gan, Yin Gu, Junping Han, Caixia Li, Jing Sun, Peng Liu
Plastic microfluidic devices with embedded chitosan-modified Fusion 5 filter paper (unmodified one purchased from GE Healthcare) have been successfully developed for DNA extraction and concentration, utilizing two different mechanisms for DNA capture: the physical entanglement of long-chain DNA molecules with the fiber matrix of the filter paper and the electrostatic adsorption of DNA to the chitosan-modified filter fibers. This new method not only provided a high DNA extraction efficiency at a pH of 5 by synergistically combining these two capture mechanisms together, but also resisted the elution of DNA from filters at a pH >8 due to the entanglement of DNA with fibers...
February 23, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28215329/biotechnological-applications-of-marine-enzymes-from-algae-bacteria-fungi-and-sponges
#2
S Parte, V L Sirisha, J S D'Souza
Diversity is the hallmark of all life forms that inhabit the soil, air, water, and land. All these habitats pose their unique inherent challenges so as to breed the "fittest" creatures. Similarly, the biodiversity from the marine ecosystem has evolved unique properties due to challenging environment. These challenges include permafrost regions to hydrothermal vents, oceanic trenches to abyssal plains, fluctuating saline conditions, pH, temperature, light, atmospheric pressure, and the availability of nutrients...
2017: Advances in Food and Nutrition Research
https://www.readbyqxmd.com/read/28208574/complexation-of-phytochemicals-with-cyclodextrin-derivatives-an-insight
#3
REVIEW
Vasanti Suvarna, Parul Gujar, Manikanta Murahari
Natural compounds have been attracting huge attention because of their broad therapeutic properties with specificity in their action in human health care as functional foods, pharmaceuticals and nutraceuticals. However poor bioavailability and reduced bioactivity attributed to poor solubility and instability is the major drawback hindering the incorporation of these therapeutically potential molecules in novel drug delivery systems. Based on the findings of reported research investigations; complexation of poorly water soluble phytochemicals with cyclodextrins has emerged to be a promising approach to improve their aqueous solubility, stability, rate of dissolution and bioavailability...
February 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28199121/smart-contact-lenses-with-graphene-coating-for-electromagnetic-interference-shielding-and-dehydration-protection
#4
Sangkyu Lee, Insu Jo, Sangmin Kang, Bongchul Jang, Joonhee Moon, Jong Bo Park, Soochang Lee, Sichul Rho, Youngsoo Kim, Byung Hee Hong
Recently, smart contact lenses with electronic circuits have been proposed for various sensor and display applications where the use of flexible and biologically stable electrode materials is essential. Graphene is an atomically thin carbon material with a two-dimensional hexagonal lattice that shows outstanding electrical and mechanical properties as well as excellent biocompatibility. In addition, graphene is capable of protecting eyes from electromagnectic (EM) waves that may cause eye diseases such as cataracts...
February 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28190945/mems-based-flexible-force-sensor-for-tri-axial-catheter-contact-force-measurement
#5
Hardik J Pandya, Jun Sheng, Jaydev P Desai
Atrial fibrillation (AFib) is a significant healthcare problem caused by the uneven and rapid discharge of electrical signals from pulmonary veins (PVs). The technique of radiofrequency (RF) ablation can block these abnormal electrical signals by ablating myocardial sleeves inside PVs. Catheter contact force measurement during RF ablation can reduce the rate of AFib recurrence, since it helps to determine effective contact of the catheter with the tissue, thereby resulting in effective power delivery for ablation...
February 2017: Journal of Microelectromechanical Systems
https://www.readbyqxmd.com/read/28190399/a-community-based-survey-of-toxoplasma-gondii-infection-among-pregnant-women-in-rural-areas-of-taiz-governorate-yemen-the-risk-of-waterborne-transmission
#6
Mohammed A K Mahdy, Lina M Q Alareqi, Rashad Abdul-Ghani, Samira M A Al-Eryani, Abdullah A Al-Mikhlafy, Abdulsalam M Al-Mekhlafi, Fawzya Alkarshy, Rohela Mahmud
BACKGROUND: Toxoplasma gondii is a zoonotic coccidian parasite causing morbidity and mortality. In Yemen, T. gondii infection has been reported among pregnant women seeking healthcare in the main cities. However, no data are available on the prevalence of T. gondii infection and its associated risk factors among pregnant women in the rural communities of the country. Thus, the present study aimed to determine the seroprevalence of T. gondii and identify its risk factors among pregnant women in the rural communities of Taiz governorate, Yemen...
February 13, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28156650/organizational-factors-influencing-specialty-palliative-care-utilization-at-academic-cancer-centers
#7
Michael W Rabow, Megan Crowley-Makota, Mallory Marie Waters, Michelle Nkumsah, Douglas B White, Edward Chu, Cardinale B Smith, Robert Arnold
: 109 Background: Rates of early specialty palliative care utilization remain low, despite increasing familiarity and favorable attitudes amongst cancer center oncologists. In this context, understanding organizational barriers to specialty palliative care use becomes critical. METHODS: We conducted a qualitative interview study with 74 medical oncologists practicing at 3 U.S. academic cancer centers with well-established specialty palliative care clinics. The in-depth interview guide elicited experiences and views related to outpatient palliative care...
October 9, 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28155287/primary-health-workers-knowledge-and-practices-relating-to-neonatal-jaundice-in-ibadan-nigeria
#8
Adebola E Orimadegun, Adeola O Ojebiyi
BACKGROUND: Over half of births and newborn care occur in primary healthcare facilities in Nigeria, but information on activities of personnel working there is scarce. AIM: To assess the knowledge and practices relating to neonatal jaundice (NNJ) among community health workers (CHWs) and community birth attendants (CBAs) in Nigeria. SETTING: We conducted a cross-sectional survey of all 227 CHWs and 193 registered CBAs in Ibadan, Nigeria...
January 30, 2017: African Journal of Primary Health Care & Family Medicine
https://www.readbyqxmd.com/read/28155024/an-analysis-of-under-5-mortality-determinants-across-countries-towards-defining-priorities-for-achieving-target-in-sustainable-development-goals
#9
Michael Acheampong, Chukwudi Ejiofor, Abraham Salinas-Miranda
Objectives The end of the era of millennium development goals (MDGs) ushered in the sustainable development goals (SDGs) with a new target for the reduction of under-five mortality rates (U5MR). Although U5MR decreased globally, the reduction was insufficient to meet MDGs targets because significant socioeconomic inequities remain unaddressed across and within countries. Thus, further progress in achieving the new SDGs target will be hindered if there is no adequate prioritization of important socioeconomic, healthcare, and environmental factors...
February 2, 2017: Maternal and Child Health Journal
https://www.readbyqxmd.com/read/28143462/a-study-on-the-influence-of-internet-addiction-and-online-interpersonal-influences-on-health-related-quality-of-life-in-young-vietnamese
#10
Bach Xuan Tran, Le Thi Huong, Nguyen Duc Hinh, Long Hoang Nguyen, Bao Nguyen Le, Vuong Minh Nong, Vu Thi Minh Thuc, Tran Dinh Tho, Carl Latkin, Melvyn Wb Zhang, Roger Cm Ho
BACKGROUND: Internet addiction (IA) is a common problem found in young Asians. This study aimed to study the influence of IA and online activities on health-related quality of life (HRQOL) in young Vietnamese. This study also compared the frequencies of anxiety, depression and other addiction of young Vietnamese with and without IA. METHODS: This study recruited 566 young Vietnamese (56.7% female, 43.3% male) ranging from 15 to 25 years of age via the respondent-driven sampling technique...
January 31, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28112087/tackling-the-climate-targets-set-by-the-paris-agreement-cop-21-green-leadership-empowers-public-hospitals-to-overcome-obstacles-and-challenges-in-a-resource-constrained-environment
#11
E Weimann, B Patel
The healthcare sector itself contributes to climate change, the creation of hazardous waste, use of toxic metals such as mercury, and water and air pollution. To mitigate the effect of healthcare provision on the deteriorating environment and avoid creating further challenges for already burdened health systems, Global Green Hospitals was formed as a global network. Groote Schuur Hospital (GSH), as the leading academic hospital in Africa, joined the network in 2014. Since then, several projects have been initiated to reduce the amount of general waste, energy consumption and food waste, and create an environmentally friendlier and more sustainable hospital in a resource-constrained public healthcare setting...
December 21, 2016: South African Medical Journal, Suid-Afrikaanse Tydskrif Vir Geneeskunde
https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#12
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28099965/pilot-test-of-a-new-personal-health-system-integrating-environmental-and-wearable-sensors-for-telemonitoring-and-care-of-elderly-people-at-home-smarta-project
#13
Lucia Pigini, Gabriele Bovi, Claudia Panzarino, Valerio Gower, Maurizio Ferratini, Giuseppe Andreoni, Roberto Sassi, Massimo W Rivolta, Maurizio Ferrarin
BACKGROUND: The increase in life expectancy is accompanied by a growing number of elderly subjects affected by chronic comorbidities, a health issue which also implies important socioeconomic consequences. Shifting from hospital or community dwelling care towards a home personalized healthcare paradigm would promote active aging with a better quality of life, along with a reduction in healthcare-related costs. OBJECTIVE: The aim of the SMARTA project was to develop and test an innovative personal health system integrating standard sensors as well as innovative wearable and environmental sensors to allow home telemonitoring of vital parameters and detection of anomalies in daily activities, thus supporting active aging through remote healthcare...
January 19, 2017: Gerontology
https://www.readbyqxmd.com/read/28095538/exogenous-factors-in-skin-barrier-repair
#14
Julia Schwartz, Adam J Friedman
The stratum corneum (SC) is the skin's outermost layer and serves the primary function of acting as a shield to keep foreign matter out and to essential elements, such as moisture and water, in. Maintenance of this skin barrier is crucial to healthy functioning skin. A damaged or diseased skin barrier is vulnerable to infection, irritants, and allergens. The cornerstone of skin barrier regulation and repair is through the use of moisturizers. While healthcare providers and patients may underestimate the importance of moisturizers due to their lack of active ingredients, the benefit of a well-planned moisturizer regimen for skin barrier regulation should not be discounted...
November 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28080026/ionic-strength-responsive-sulfonated-polystyrene-opals
#15
Luca Nucara, Vincenzo Piazza, Francesco Greco, Valentina Robbiano, Valentina Cappello, Mauro Gemmi, Franco Cacialli, Virgilio Mattoli
Stimuli-responsive photonic crystals (PCs) represent an intriguing class of smart materials very promising for sensing applications. Here, selective ionic strength responsive polymeric PCs are reported. They are easily fabricated by partial sulfonation of polystyrene opals, without using toxic or expensive monomers and etching steps. The color of the resulting hydrogel-like ordered structures can be continuously shifted over the entire visible range (405-760 nm) by changing the content of ions over an extremely wide range of concentration (from about 70 μM to 4 M)...
January 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28077517/two-phase-hospital-associated-outbreak-of-mycobacterium-abscessus-investigation-and-mitigation
#16
Arthur W Baker, Sarah S Lewis, Barbara D Alexander, Luke F Chen, Richard J Wallace, Barbara A Brown-Elliott, Pamela J Isaacs, Lisa C Pickett, Chetan B Patel, Peter K Smith, John M Reynolds, Jill Engel, Cameron R Wolfe, Carmelo A Milano, Jacob N Schroder, Robert D Davis, Matthew G Hartwig, Jason E Stout, Nancy Strittholt, Eileen K Maziarz, Jennifer Horan Saullo, Kevin C Hazen, Richard J Walczak, Ravikiran Vasireddy, Sruthi Vasireddy, Celeste M McKnight, Deverick J Anderson, Daniel J Sexton
BACKGROUND: Nontuberculous mycobacteria (NTM) commonly colonize municipal water and cause healthcare-associated outbreaks. We investigated a biphasic outbreak of Mycobacterium abscessus at a tertiary care hospital. METHODS: Case patients had recent hospital exposure and laboratory-confirmed colonization or infection with M. abscessus from January 2013 through December 2015. We conducted a multidisciplinary epidemiologic, field, and laboratory investigation. RESULTS: The incidence rate of M...
January 11, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28068263/chemical-burn-to-the-gingiva-after-misuse-of-an-over-the-counter-oral-whitening-mouthwash
#17
John K Brooks
A well-recognized consequence of the use of tooth whitening gels and strips, particularly prescription strength formulations, is gingival irritation and tooth sensitivity. Limited information is available in the literature on the risk of gingival chemical burns associated with over-the-counter (OTC) oral whitening rinses. This case report details the development of gingival chemical burns consequent to misuse of a whitening rinse. The affected patient had rinsed for 30 seconds daily for 3-4 days but had failed to rinse with water after usage, as specified by the manufacturer...
January 2017: General Dentistry
https://www.readbyqxmd.com/read/28050472/use-of-lung-ultrasound-for-diagnosing-acute-heart-failure-in-emergency-department-of-southern-india
#18
Manav Aggarwal, Mrigakshi Gupta, Vikrant Vijan, Anjith Vupputuri, Sanjeev Chintamani, Bishnukiran Rajendran, Rajesh Thachathodiyal, Rajiv Chandrasekaran
INTRODUCTION: Diagnosing heart failure is often a challenge for the healthcare providers due to it's non-specific and usually subtle physical presentations. The outcomes for treatment are strongly related to the stage of the disease. Considering the importance of early and accurate diagnosis, it is important to have an easy, inexpensive, non-invasive, reliable and reproducible method for diagnosis of heart failure. Recent advancement in radiology and cardiology are supporting the emerging technique of lung ultrasound through B-line evaluation for identifying extravascular lung water...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28049291/unique-triphenylphosphonium-derivatives-for-enhanced-mitochondrial-uptake-and-photodynamic-therapy
#19
Zhang Hu, Ying Sim, Oi Lian Kon, Wai Har Ng, António J M Ribeiro, Maria J Ramos, Pedro A Fernandes, Rakesh Ganguly, Bengang Xing, Felipe García, Edwin K L Yeow
In this study, unique methyl-functionalized derivatives (T*PP(+)) of the drug carrier triphenylphosphonium (TPP(+)) that exhibit significant enhancement of the accumulation of both the cation and its conjugated cargo in cell mitochondria are designed. We show that the presence of methyl group(s) at key positions within the phenyl ring results in an increase in the hydrophobicity and solvent accessible surface area of T*PP(+). In particular, when the para position of the phenyl ring in T*PP(+) is functionalized with a methyl group, the cation is most exposed to the surrounding environment, leading to a large decrease in water entropy and an increase in the level of van der Waals interaction with and partition into a nonpolar solvent...
January 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28048440/su-g-iep2-12-the-effect-of-iterative-reconstruction-and-ct-tube-voltage-on-hounsfield-unit-values-of-iodinated-contrast
#20
K Ogden, K Greene-Donnelly, D Vallabhaneni, E Scalzetti
PURPOSE: To investigate the effects of changing iterative reconstruction strength and tube voltage on Hounsfield Unit (HU) values of varying concentrations of Iodinated contrast medium in a phantom. METHOD: Iodinated contrast (Omnipaque 300, GE Healthcare, Princeton NJ) was diluted with distilled water to concentrations of 0.6, 0.9, 1.8, 3.6, 7.2, and 10.8 mg/mL of Iodine. The solutions were scanned in a patient equivalent water phantom on two MDCT scanners: VCT 64 slice (GE Medical Systems, Waukesha, WI) and an Aquilion One 320 slice scanner (Toshiba America Medical Systems, Tustin CA)...
June 2016: Medical Physics
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